CN103103062A - Method for preparing nutritional mulberry beverage from composite probiotics - Google Patents
Method for preparing nutritional mulberry beverage from composite probiotics Download PDFInfo
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- CN103103062A CN103103062A CN 201310025706 CN201310025706A CN103103062A CN 103103062 A CN103103062 A CN 103103062A CN 201310025706 CN201310025706 CN 201310025706 CN 201310025706 A CN201310025706 A CN 201310025706A CN 103103062 A CN103103062 A CN 103103062A
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Abstract
The invention discloses a method for preparing a nutritional mulberry beverage from composite probiotics. The method for preparing the mulberry nutritional beverage from composite probiotics is characterized by comprising the following steps of: carrying out pure culturing on multiple kinds of microorganisms with different functions in different fermentation devices, thereby obtaining a great amount of thalluses; and carrying out composite fermentation mulberry juice which is taken as a raw material, thereby obtaining the nutritional mulberry beverage which is rich in probiotics and metabolites thereof, and contains activity of probiotics, metabolites and full nutritional substances of mulberry fruits at the same time, Multiple microorganisms which have different growth speeds and cycles, mutually complementary nutrition and can be contained in the same environment are respectively adopted according to the physiological characteristics of cultures; a great amount of thalluses are obtained through the pure culturing; the culture purity of composite fermentation is ensured; and the method for preparing the mulberry nutritional beverage from composite probiotics has the advantages that the growth of cancer cells is inhibited by certain genes in human bodies, embolism in blood cells is prevented, and multiple health care efficacies are generated, and is suitable for the production of the nutritional mulberry beverage prepared from composite probiotics.
Description
Technical field
The present invention is specifically related to a kind of compound probiotic and prepares mulberries nutritional drink method, belong to technical field of biological fermentation, to adopt the several functions probiotic bacterium, purebred cultivation, obtain a large amount of thalline, take morisuccus as raw material, complex ferment finally obtains a kind of probiotic bacterium and meta-bolites mulberries nutritional drink thereof of being rich in.
Background technology
Composite probiotics preparations, the product that is considered to have the microorganism complex function.The common fermentation of compound probiotic, its meaning also are probiotic bacterium vital movement each other, the material of generation or the environment that causes, and interdependence benefits mutually.Thereby obtain maximum thalline quantity and best meta-bolites.This series products is widely used in the fields such as medicine, health care, agricultural, livestock industry take microorganism products as representative.The combination of probiotic bacterium and the condition of fermenting altogether are the key problem in technology of quality product.Mulberries are nutritious, are rich in 16 seed amino acids such as Methionin, glutamine, Histidine, contain again multivitamin and the organic acids such as VB, Vc, VE, also contain the mineral elements such as Fe, Ca, Zn and Hu Luosu, Mierocrystalline cellulose, pectin etc.Modern medicine study proposes mulberries 6 large disease-prevention health functions, the antimutagenic that namely gives protection against cancer, and strengthening immunity is protected kidney and is protected liver, preserves youthful looks anti-ageing, promotes the hemocyte growth, reduces blood pressure and blood lipoid.It is worth noting especially the material that contains a kind of crying " resveratrol " in the poly-fruit of mulberries, it can stimulate some gene inhibition growth of cancer cells in human body, and can stop the formation of embolism in blood cell.Mulberries are worth because its various health care functions has exploitation, simultaneously, if mulberries can be fully utilized as the byproduct of sericulture, can also improve mulberry planter's income, have significant social benefit.
Summary of the invention
The purpose of this invention is to provide some gene inhibition growth of cancer cells in a kind of stimulation human body, stop the formation of embolism in blood cell.Have various health care functions and have the compound probiotic that exploitation is worth and prepare mulberries nutritional drink method
Main technical schemes of the present invention is achieved in that described compound probiotic prepares mulberries nutritional drink method: adopt multiple different functionalities probiotic bacterium, through purebred cultivation, obtain a large amount of thalline.With the morisuccus raw material, carry out the complex ferment of probiotic bacterium, a kind of mulberries nutritional drink that is rich in probiotic bacterium and meta-bolites thereof of final acquisition, compound probiotic prepares mulberries nutritional drink method, comprise function yeast selection of species, cultivation and combination, culture medium prescription and preparation, the control design of culture condition.
It is characterized in that taking respectively the speed of growth and the cycle is different, nutrition complement, the probiotic bacterium that can reside in altogether same environment each other according to the physilogical characteristics of bacterial classification, through pure culture, obtain a large amount of probiotic bacterium thalline.
Described bacterial classification comprises the multiple beneficial microorganisms such as bacillus, genus lactubacillus, Saccharomycodes, acetic acid Pseudomonas.Have the functions such as the various active of producing enzyme, multiple organic acid, VITAMIN.Be widely used in the health care of food industry.
Described pure culture is to adopt the general deep liquid culture tank of fermentation industry, selects the substratum of suitable growth according to the needs of each bacterial classification, and aeration condition is cultivated, and thalline reaches maximum quantity, and maturation is put tank.
Described complex ferment adopts deep liquid standing for fermentation mode, take morisuccus as appropriate media, is mainly under anaerobic to make its fermentation, obtains the viable bacteria of some amount and is rich in the tunning of morisuccus nutrition.
The slant medium that described process for preparing strain thereof is used has following component and better weight proportion: peptone 8～12g extractum carnis 4～6g glucose 1～3g agar 12～20g distilled water 1L.
The substratum that described bacterial classification liquid culture is used has following component and better weight proportion:
Soybean hydrolyzate 10～30g peptone 4～10g sucrose 16～24g KH
2PO
43～8gMgSO
41～6g water 1L.
The standing fermention medium altogether of described bacterial classification deep liquid has following component and better weight proportion:
Sucrose 10～30g peptone 4～10g yeast extract paste 5-10g KH
2PO
45～10g MgSO
41～6g morisuccus 1L.
Positively effect of the present invention is: compound probiotic pure growth in different incubators, in same incubator, ferment altogether by morisuccus, and obtain a kind of mulberries nutritional drink that is rich in multiple probiotic bacterium and meta-bolites thereof.
Compared with prior art: the present invention is at first with the microorganism of multiple specific function, and purebred cultivation obtains the active somatic cells of a large amount of microorganisms.Obtain compound probiotic mulberries nutritional drink through complex ferment.The pure culture of probiotic bacterium has guaranteed the physiologically active of common fermented bacterium.Bacterial classification purity has improved the security of product.
Be described further below in conjunction with example, but not as a limitation of the invention.
Embodiment
Example 1.
Slant strains: the Bacillus licheniformis inclined-plane, in 25-32 ℃ of constant temperature culture, 24-72h.
Bacteria suspension: with the bacterial classification test tube slant, pour respectively sterilized water into, scraping lawn, preparation bacteria suspension.
Liquid nutrient medium: the substratum that the bacterial classification liquid culture is used has following component and weight proportion: soybean hydrolyzate 10～30g peptone 4～10g sucrose 16～24gKH
2PO
43～8g MgSO
41～6g water 1L.
Sterilization: 0.12-0.15Mpa, 121-125 ℃, steam sterilizing 30 minutes.
Inoculation: in the substratum with bacteria suspension access sterilization.
Cultivate: 25-32 ℃, tank pressure 0.04-0.05Mpa cultivates 36-72h.Get bacterium liquid 3..
Example 2.
Slant strains: milk-acid bacteria 1., yeast 2. the test tube slant in 25-38 ℃ of constant temperature culture, 24-72h.
Bacteria suspension: with 1. 2. test tube slant of bacterial classification, pour respectively sterilized water into, scraping lawn, preparation bacteria suspension.
Bacterial classification mixes: with 1. 2. bacteria suspension of bacterial classification, be mixed in proportion.
Liquid nutrient medium: sucrose 10～30g peptone 4～10g yeast extract paste 5-10g KH
2PO
45～10g MgSO
41～6g water 1L
Sterilization: 0.12-0.15Mpa, 121-125 ℃, steam sterilizing 30 minutes.
Inoculation: in the substratum with bacteria suspension access sterilization.
Cultivate: 25-32 ℃, cultivate 2-7d.
Example 3.
The compound probiotic bacterium that example 1,2 is obtained of bacterium liquid is compound in the ratio of 0.1: 10, preparation composite bacteria suspension.
Fermention medium: sucrose 10～30g peptone 4～10g yeast extract paste 5-10g KH
2PO
45～10g MgSO
41～6g morisuccus 1L.
Sterilization: 0.11-0.12Mpa, 115-120 ℃, steam sterilizing 30 minutes.
Inoculation: in the substratum with composite bacteria suspension access sterilization.
Cultivate: 25-32 ℃, cultivate 2-7d.
Claims (5)
1. compound probiotic prepares mulberries nutritional drink method, comprise function yeast selection of species, combination and cultivation, the control of culture medium prescription and preparation, culture condition and design, it is characterized in that taking respectively the speed of growth and the cycle is different, nutrition complement, two kinds of microorganisms can residing in altogether same environment each other according to the physilogical characteristics of bacterial classification, through purebred cultivation, obtain to contain the microbial cells of several functions.
2. compound probiotic according to claim 1 prepares mulberries nutritional drink method, it is characterized in that adopting and comprise bacillus, genus lactubacillus, Saccharomycodes, acetic acid Pseudomonas, light and the multiple beneficial microorganisms such as bacterium and thread fungus, have the functions such as the various active of producing enzyme, multiple organic acid, VITAMIN, select the inoculative proportion of purebred cultivation: 0.1-2: 1-10.
3. compound probiotic according to claim 2 prepares mulberries nutritional drink method, it is characterized in that having in described substratum the ratio of soybean hydrolyzate and each component: soybean hydrolyzate 10～50g peptone 4～10g sucrose 16～24g KH
2PO
43～8g MgSO
41～6g water 1L.
4. compound probiotic according to claim 2 prepares mulberries nutritional drink method, it is characterized in that adopting bacterial classification in vital movement, can produce various active enzyme and secondary metabolite, as the microorganism of multiple organic acid, small-molecular peptides, VITAMIN, oligosaccharide substance.Select the standing anaerobism training method of deep layer, complex ferment.Inoculum size proportioning: 1: 5-40.
5. compound probiotic according to claim 4 prepares mulberries nutritional drink method, it is characterized in that having following component and weight proportion in described substratum: sucrose 10～30g peptone 4～10g yeast extract paste 5-10g KH
2PO
45～10g MgSO
41～6g morisuccus 1L.
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| CN 201310025706 CN103103062A (en) | 2013-01-10 | 2013-01-10 | Method for preparing nutritional mulberry beverage from composite probiotics |
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| CN 201310025706 CN103103062A (en) | 2013-01-10 | 2013-01-10 | Method for preparing nutritional mulberry beverage from composite probiotics |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981078A (en) * | 2014-05-12 | 2014-08-13 | 广东庭颢药业股份有限公司 | Mulberry vinegar and preparation method thereof |
| CN104031792A (en) * | 2014-05-08 | 2014-09-10 | 贾秉堂 | Mulberry health-care wine and preparation method thereof |
| CN109007498A (en) * | 2018-07-28 | 2018-12-18 | 普定县真源农业开发有限公司 | A kind of preparation method of mulberry juice |
-
2013
- 2013-01-10 CN CN 201310025706 patent/CN103103062A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031792A (en) * | 2014-05-08 | 2014-09-10 | 贾秉堂 | Mulberry health-care wine and preparation method thereof |
| CN103981078A (en) * | 2014-05-12 | 2014-08-13 | 广东庭颢药业股份有限公司 | Mulberry vinegar and preparation method thereof |
| CN109007498A (en) * | 2018-07-28 | 2018-12-18 | 普定县真源农业开发有限公司 | A kind of preparation method of mulberry juice |
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Application publication date: 20130515 |