CN104450599A - Probiotic corn leavening agent capable of increasing content of L-lactic acid and application of probiotic corn leavening agent - Google Patents
Probiotic corn leavening agent capable of increasing content of L-lactic acid and application of probiotic corn leavening agent Download PDFInfo
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Abstract
The invention provides a probiotic corn leavening agent capable of increasing the content of L-lactic acid and the application of the probiotic corn leavening agent. The probiotic corn leavening agent is prepared from such components as lactobacillus delbrueckii, lactobacillus crispatus, pediococcus acidilactici, endomycopsis, saccharomyces uvarum, bacillus amyloliquefaciens, alpha-amylase, NH4CL accounting for 0.2%-0.25% of the mass of the leavening agent, Na2HPO4.12H2O accounting for 0.15%-0.2% of the mass of the leavening agent, and KH2PO4 accounting for 0.4%-0.5% of the mass of the leavening agent. The probiotic corn leavening agent is inoculated to corn in the mass ratio of 0.1%, and then the moisture content of materials is regulated to 40%, the fermentation temperature is controlled at 37 DEG C and the fermentation time is kept at 60 hours, and therefore, the content of the L-lactic acid in corn can be remarkably increased, the pH can be reduced and the palatability can be improved; besides, rapid breeding of a plurality of probiotics such as lactic acid bacteria, saccharomycetes and bacillus also can be promoted.
Description
Technical field
The invention belongs to biological enzymolysis and fermentation arts, relate to corn fermentation agent prescription, be specifically related to a kind of agent of probiotic bacterium corn fermentation and the application thereof that improve L-lactic acid.
Background technology
Corn nourishment enriches, and wherein containing protein 9.88%, fat 5.21%, robust fibre 3.20% etc., can be used as a kind of energy feed of high-quality, aquaculture is widely used.But because young animal gi tract are not also grown completely, amylase activity is low, utilizes limited to complex carbohydrates, thus low to the capacity usage ratio of corn, even causes the problems such as diarrhoea, cessation of growth cessation.
In current corn processing methods, microbe fermentation method is the most general.The various enzymes that microorganism produces in process of growth, can reduce the relative molecular mass of feed Middle nutrition material, make it advantageously digest and assimilate in young animal.The present invention is in conjunction with moral formula Bacterium lacticum, lactobacillus crispatus, pediococcus acidilactici, endomycopsi.sp, saccharomyces uvarum bacterium, the multiple probiotic bacterium of bacillus amyloliquefaciens and α-amylase, be aided with other nutritive substances, can complex carbohydrates effectively in degrading maize, remarkable reduction corn pH, improve corn L-lactic acid, and promote the rapid breeding of the multiple probiotic bacteriums such as milk-acid bacteria, yeast, genus bacillus.Lactic acid is a kind of efficient, noresidue, free of contamination fodder additives, and have the effect promoting growth of animal, extend the feed quality guaranteed period, prevent feed mold, the application in feed is more and more extensive.The production of lactic acid mainly contains biological fermentation process and chemical synthesis, and current chemical method is gradually by microbe fermentation method is replaced.
Summary of the invention
The invention provides a kind of agent of probiotic bacterium corn fermentation and the application thereof that improve L-lactic acid, utilize starter of the present invention can significantly improve corn L-lactic acid, reduce pH, improve palatability, and promote the rapid breeding of the multiple probiotic bacteriums such as milk-acid bacteria, yeast, genus bacillus.
For achieving the above object, the present invention is achieved by the following technical solutions:
Improve a probiotic bacterium corn fermentation agent prescription for L-lactic acid, it comprises following composition: bacteria containing amount is 5 × 10
6~ 6 × 10
6the moral formula Bacterium lacticum of CFU/g; Bacteria containing amount is 1 × 10
6~ 2 × 10
6the lactobacillus crispatus of CFU/g; Bacteria containing amount is 1 × 10
6~ 2 × 10
6the pediococcus acidilactici of CFU/g; Bacteria containing amount is 5 × 10
4~ 1 × 10
5the endomycopsi.sp of CFU/g; Bacteria containing amount is 5 × 10
4~ 1 × 10
5the saccharomyces uvarum bacterium of CFU/g; Bacteria containing amount is 5 × 10
4~ 8 × 10
4the bacillus amyloliquefaciens of CFU/g; Enzyme content is the α-amylase of 400 ~ 500U/g; Account for the NH that starter mass ratio is 0.2% ~ 0.25%
4cL; Account for the Na that starter mass ratio is 0.15% ~ 0.2%
2hPO
4˙ 12H
2o; Account for the KH that starter mass ratio is 0.4% ~ 0.5%
2pO
4.
Further improvement to technique scheme: the optimum bacteria containing amount of described moral formula Bacterium lacticum is 5 × 10
6cFU/g.
Further improvement to technique scheme: described corn fermentation agent with account for corn mass ratio be 0.1% ratio be inoculated in corn, be placed in double-layer seal bag, carry out constant-temperatureanaerobic anaerobic fermentation.
Further improvement to technique scheme: described corn fermentation agent is inoculated in corn, needs the ratio of adjusting corn and water, makes moisture content of material reach 40%, to promote that nutritive substance discharges and microorganism ramp.
Further improvement to technique scheme: described corn fermentation agent is inoculated in corn, and leavening temperature controls at 37 DEG C.
Further improvement to technique scheme: described corn fermentation agent is inoculated in corn, and fermentation time remains on 60h.
Compared with prior art, the advantage of the inventive method and technique effect are:
1, traditional corn souring agent, only can reduce corn pH, and probiotic bacterium corn fermentation of the present invention agent, while reduction pH, also can significantly improve L-lactic acid in corn, and feed palatability can be improved.
2, this described corn fermentation agent of fermenting, in the process of fermented maize, also can promote the rapid breeding of the multiple probiotic bacteriums such as milk-acid bacteria, yeast, genus bacillus, a considerable number of, also can be used as probiotics and applies in feed.
3, bacterium involved in the present invention, enzyme etc., all belong to fodder additives category, wide material sources, and safety is easy to get, with low cost.
After reading the specific embodiment of the present invention by reference to the accompanying drawings, other advantages of the present invention will become clearly.
Accompanying drawing explanation
Fig. 1 to show under different vaccination amount L-lactic acid after corn fermentation.
Fig. 2 to show under different in moisture L-lactic acid after corn fermentation.
Fig. 3 shows the change of L-lactic acid with corn fermentation temperature.
Fig. 4 shows the change of L-lactic acid with the corn fermentation time.
Embodiment
For understanding the function of this compound benefit bacterium peptide product better, below in conjunction with the drawings and specific embodiments, feature being quoted to product of the present invention and being further described in detail.
In the system component of corn fermentation agent of the present invention, moral formula Bacterium lacticum, lactobacillus crispatus, pediococcus acidilactici, endomycopsi.sp, saccharomyces uvarum bacterium, bacillus amyloliquefaciens are the bacterium powder of commercial goods, the zymin that the α-amylase used is commercial goods, and described NH
4cL is analytical pure, purchased from economic and technological development zone, Laiyang Fine Chemical Works, and Na
2hPO
4 ˙12H
2o and KH
2pO
4be analytical pure, purchased from Chemical Reagent Co., Ltd., Sinopharm Group.
Embodiment 1
The blending ratio of adjusting corn and water, controlling moisture content of material is 25%; Control group only adds water; Test group 1, on the basis of control group, with the mass ratio of 1%, inoculates citric acid; Test group 2, on the basis of control group, with the mass ratio of 0.1%, inoculates probiotic bacterium corn fermentation of the present invention agent.Often organize 3 repetitions.
After mixing of materials is even, be placed in double-layer seal bag, 37 DEG C of anaerobically fermenting 72h.Utilize PHS-3C PH to count, measure PH.Take 10g fermented sample, add 90ml water, under normal temperature, magnetic stirs the centrifugal 10min of 1h, 10000rpm, gets supernatant, utilizes SBA-40D bio-sensing analyser, measures L-lactic acid.Utilize MRS substratum, adopt tilt-pour process, cultivate 48h for 37 DEG C, measure milk-acid bacteria bacterium amount; Utilize PDA substratum, adopt method of dilution butteron on plate, cultivate 48h for 30 DEG C, measure yeast bacterium amount; Utilize nutrient agar, adopt method of dilution butteron on plate, cultivate 48h for 37 DEG C, measure sporeformer bacterium amount.Utilize SPSS software, difference analysis is carried out to testing data.
Table 1 shows the change of Different treatments to pH after corn fermentation and Pfansteihl.Compared with control group, test group-1 and test group-2 all can reduce pH, and the citric acid that test group-1 is added is conventional acid agent, and addition is large, but acidizing effect is faint, compared with control group, does not reach conspicuous level; Test group-2 adds probiotic bacterium corn fermentation of the present invention agent, and only 0.1% ratio is added, and significantly can reduce pH, play corn acidizing effect; In addition, test group-2, compared with test group-1, control group, also can significantly improve the content of Pfansteihl.
Lactic acid is a kind of important antimicrobial compounds, play an important role to the antagonism of pathogenic bacterium, it makes the pH in enteron aisle and redox-potential decline, and forms enteron aisle inner acidic environment, cause acid nonfast pathogenic bacterium dead rapidly, thus reach the object of regulating intestinal canal colony balance.
Table 2 shows the change of Different treatments to probiotics bacterial amount after corn fermentation.Compared with control group, although test group-1 can improve the bacterium amount of various probiotics bacterial, do not reach magnitude differences; After the corn fermentation that test group-2 processes, milk-acid bacteria bacterium amount adds 4 orders of magnitude, yeast adds 3 orders of magnitude, sporeformer adds 3 orders of magnitude, prolific probiotic bacterium, can regulate animal intestinal micro-ecology environment, also to a certain extent, probiotics can be substituted and apply in feed.
Table 1. Different treatments is to the change of pH after corn fermentation and Pfansteihl
Table 2. Different treatments is to the change of probiotics bacterial amount after corn fermentation
Embodiment 2
The blending ratio of adjusting corn and water, controlling moisture content of material is 25%; Control group and test group all adopt corn fermentation agent prescription, but moral formula Bacterium lacticum addition difference to some extent.Control group is not containing moral formula Bacterium lacticum, and test group-1 adds 1*10
6cFU/g moral formula Bacterium lacticum, test group-2 adds 5*10
6cFU/g moral formula Bacterium lacticum, test group-3 adds 1*10
7cFU/g moral formula Bacterium lacticum.With the mass ratio of 0.1%, by control group and campaign group of formula, inoculate probiotic bacterium corn fermentation of the present invention agent, investigate moral formula Bacterium lacticum core component to the impact of L-lactic acid after corn fermentation.Often organize 3 repetitions.After mixing of materials is even, be placed in double-layer seal bag, 37 DEG C of anaerobically fermenting 72h.Take 10g fermented sample, add 90ml water, under normal temperature, magnetic stirs the centrifugal 10min of 1h, 10000rpm, gets supernatant, utilizes SBA-40D bio-sensing analyser, measures L-lactic acid.Utilize SPSS software, difference analysis is carried out to testing data.
Table 3 shows to fill a prescription Sino-German formula Bacterium lacticum consumption to the impact of L-lactic acid after corn fermentation.Compared with not inoculating the control group of moral formula Bacterium lacticum, three test group have been significantly increased the L-lactic acid (p<0.05) of fermented maize.Compared with control group, the corn fermentation L-lactic acid amplification of test group-1 is 36.07%, amplification is lower, test group-2 is respectively 116.39% and 119.67% with the corn fermentation L-lactic acid amplification of test group-3, amplification is higher, significantly increase L-lactic acid in corn, but without significant difference (p>0.05) between test group 2 and test group 3.This test-results shows, in corn fermentation agent prescription of the present invention, adds 5*10
6cFU/g moral formula Bacterium lacticum, can significantly improve L-lactic acid, again can conservative control cost.
Table 3. fills a prescription Sino-German formula Bacterium lacticum consumption to the impact of L-lactic acid after corn fermentation
Embodiment 3
The blending ratio of adjusting corn and water, controlling moisture content of material is 25%; Adopt gradient inoculum size (mass ratio): 0.025%, 0.05%, 0.075%, 0.1%, 0.125%, 0.15%, inoculate probiotic bacterium corn fermentation of the present invention agent, investigate inoculum size to the impact of L-lactic acid after corn fermentation.Often organize 3 repetitions.After mixing of materials is even, be placed in double-layer seal bag, 37 DEG C of anaerobically fermenting 72h.Take 10g fermented sample, add 90ml water, under normal temperature, magnetic stirs the centrifugal 10min of 1h, 10000rpm, gets supernatant, utilizes SBA-40D bio-sensing analyser, measures L-lactic acid.
Fig. 1 to show under different vaccination amount L-lactic acid after corn fermentation.When inoculative proportion lower than 0.1% time, after corn fermentation, L-lactic acid improves along with improving constantly of inoculative proportion, and 0.1% compared with 0.025% inoculum size, and the L-lactic acid of fermented maize adds 236%; But after 0.1%, the increase of inoculum size, can not drive the remarkable increase of fermented maize L-lactic acid, when 0.1% inoculum size is described, substantially reaches balance stagnation point.Based on this embodiment result, when filling a prescription use, the inoculum size (mass ratio) of suggestion employing 0.1% carries out corn fermentation.
Embodiment 4
The ratio of adjusting corn and water, makes moisture content of material be respectively 25%, 30%, 35%, 40% and 45%.By the mass ratio of 0.1%, inoculate probiotic bacterium corn fermentation of the present invention agent.Often organize 3 repetitions.After mixing of materials is even, be placed in double-layer seal bag, 37 DEG C of anaerobically fermenting 72h.Take 10g fermented sample, add 90ml water, under normal temperature, magnetic stirs the centrifugal 10min of 1h, 10000rpm, gets supernatant, utilizes SBA-40D bio-sensing analyser, measures L-lactic acid.
Fig. 2 to show under different in moisture L-lactic acid after corn fermentation.Moisture is comparatively large to corn fermentation Pfansteihl Index Influence, and moisture content is higher, and L-lactic acid is higher.When moisture is higher, be conducive to nutritive substance release and microorganism growth, milk-acid bacteria bacterium amount rate of propagation is very fast, and activity is comparatively strong, and correspondingly, the output of Pfansteihl isoreactivity material is also higher.Under 40% moisture, after corn fermentation, L-lactic acid can reach 4.82%; Under 45% moisture, after corn fermentation, L-lactic acid declines to some extent, is only 4.61%, infers that reason is: too high moisture, affects heat loss, while raising fermentation materials temperature, also can affect the growth of bacterium and the increase of L-lactic acid.This test-results shows, 40% is proper moisture content of material.
Embodiment 5
The blending ratio of adjusting corn and water, controlling moisture content of material is 40%, with the mass ratio of 0.1%, inoculates probiotic bacterium corn fermentation of the present invention agent.Often organize 3 repetitions.After mixing of materials is even, is placed in double-layer seal bag, adopts different leavening temperatures respectively: 25 DEG C, 30 DEG C, 37 DEG C, 42 DEG C, 50 DEG C are carried out constant-temperatureanaerobic anaerobic fermentation, and fermentation time is 72h.Take 10g fermented sample, add 90ml water, under normal temperature, magnetic stirs the centrifugal 10min of 1h, 10000rpm, gets supernatant, utilizes SBA-40D bio-sensing analyser, measures L-lactic acid.Measure the L-lactic acid at different fermentations temperature respectively.
Fig. 3 shows the change of L-lactic acid with corn fermentation temperature.Result shows, and leavening temperature has certain influence to L-lactic acid after corn fermentation, and 37 DEG C is optimum temperuture.At 25 DEG C, temperature is lower, is unfavorable for the microbial growths such as milk-acid bacteria, and thus the metabolite output such as Pfansteihl is also lower, is only 3.80%.When leavening temperature is 30 DEG C, 37 DEG C, L-lactic acid increases trend gradually, especially the highest with L-lactic acid when 37 DEG C, infers that this temperature is the optimum growth temperature of milk-acid bacteria, facilitate the breeding of the serial microorganisms such as milk-acid bacteria, and produce a large amount of Pfansteihls.Work as increase in temperature, when reaching 42 DEG C, after corn fermentation, L-lactic acid is 4.76%, a little less than 4.83% when 37 DEG C.When temperature reaches 50 DEG C, in corn fermentation process, the breeding of microorganism is subject to high temperature suppression, and is unfavorable for the generation of probiotic meta-bolites.
Embodiment 6
The blending ratio of adjusting corn and water, controlling moisture content of material is 40%, with the mass ratio of 0.1%, inoculates probiotic bacterium corn fermentation of the present invention agent.Often organize 3 repetitions.After mixing of materials is even, be placed in double-layer seal bag, 37 DEG C of anaerobically fermenting 72h.Take 10g fermented sample, add 90ml water, under normal temperature, magnetic stirs the centrifugal 10min of 1h, 10000rpm, gets supernatant, utilizes SBA-40D bio-sensing analyser, measures L-lactic acid.Measure the L-lactic acid of different time points respectively, minute point is respectively: 0h, 6h, 12h, 18h, 24h, 30h, 36h, 42h, 48h, 54h, 60h, 66h, 72h.
Fig. 4 shows the change of L-lactic acid with the corn fermentation time.At front 24h, L-lactic acid slowly increases, and amplification is less, is only 0.67%; From 24h to 36h, corn L-lactic acid increases rapidly, and amplification is comparatively large, reaches 3.17%; From 36h to 72h, the growth of corn L-lactic acid becomes again slow, to reaching the highest L-lactic acid 4.82% during 60h, remains unchanged afterwards; Improve the angle of L-lactic acid from corn fermentation, the time of corn fermentation remains on 60h.
Corn fermentation agent of the present invention with account for corn mass ratio be 0.1% ratio be inoculated in commercially available common Semen Maydis powder, be placed in double-layer seal bag, carry out constant-temperatureanaerobic anaerobic fermentation, and regulating moisture content of material to 40%, it is 37 DEG C that leavening temperature controls, and fermentation time remains on 60h, L-lactic acid in corn can be significantly improved, and can pH be reduced, improve palatability, also can promote the rapid breeding of the multiple probiotic bacteriums such as milk-acid bacteria, yeast, genus bacillus.
Adopt probiotics leaven fermented maize of the present invention, achieve the symplastic growth of several bacterium such as yeast, bacillus amyloliquefaciens such as the milk-acid bacterias such as moral formula Bacterium lacticum, lactobacillus crispatus, pediococcus acidilactici, endomycopsi.sp, saccharomyces uvarum bacterium, and promote the increase gradually of corn L-lactic acid.Separate the ramp of starch sporeformer early stage, and act on α-amylase simultaneously, the nutritive substances such as release starch, dextrin, soluble sugar, when oxygen content lowers gradually, the amphimicrobes such as yeast growth and breeding gradually, middle and later periods oxygen depletion completely after proceed to fermentation pattern, and the milk-acid bacterias such as moral formula Bacterium lacticum also facultative with fast growth and breeding under anaerobic condition.In whole fermenting process, corn can be used as carbon source and nitrogenous source, and plurality of inorganic salt provides a large amount of and micro-, achieves the relay growth and breeding of multiple-microorganism, and promotes increasing sharply of corn L-lactic acid.
Above embodiment only in order to technical scheme of the present invention to be described, but not is limited; Although with reference to previous embodiment to invention has been detailed description, for the person of ordinary skill of the art, still can modify to the technical scheme described in previous embodiment, or equivalent replacement is carried out to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (6)
1. improve a probiotic bacterium corn fermentation agent for L-lactic acid, it is characterized in that it comprises following composition: bacteria containing amount is 5 × 10
6~ 6 × 10
6the moral formula Bacterium lacticum of CFU/g; Bacteria containing amount is 1 × 10
6~ 2 × 10
6the lactobacillus crispatus of CFU/g; Bacteria containing amount is 1 × 10
6~ 2 × 10
6the pediococcus acidilactici of CFU/g; Bacteria containing amount is 5 × 10
4~ 1 × 10
5the endomycopsi.sp of CFU/g; Bacteria containing amount is 5 × 10
4~ 1 × 10
5the saccharomyces uvarum bacterium of CFU/g; Bacteria containing amount is 5 × 10
4~ 8 × 10
4the bacillus amyloliquefaciens of CFU/g; Enzyme content is the α-amylase of 400 ~ 500 U/g; Account for the NH that starter mass ratio is 0.2% ~ 0.25%
4cL; Account for the Na that starter mass ratio is 0.15% ~ 0.2%
2hPO
412H
2o; Account for the KH that starter mass ratio is 0.4% ~ 0.5%
2pO
4.
2. probiotic bacterium corn fermentation according to claim 1 agent, is characterized in that: the optimum bacteria containing amount of described moral formula Bacterium lacticum is 5 × 10
6cFU/g.
3. the application of probiotic bacterium corn fermentation agent in fermented maize described in claim 1 or 2, is characterized in that: described corn fermentation agent with account for corn mass ratio be 0.1% ratio be inoculated in corn, be placed in double-layer seal bag, carry out constant-temperatureanaerobic anaerobic fermentation.
4. the application of probiotic bacterium corn fermentation according to claim 3 agent in fermented maize, is characterized in that: described corn fermentation agent is inoculated in corn, and the ratio of adjusting corn and water makes moisture content of material reach 40%.
5. the application of probiotic bacterium corn fermentation according to claim 3 agent in fermented maize, is characterized in that: described corn fermentation agent is inoculated in corn, and leavening temperature controls at 37 DEG C.
6. the application of probiotic bacterium corn fermentation according to claim 3 agent in fermented maize, is characterized in that: described corn fermentation agent is inoculated in corn, and fermentation time remains on 60h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872379A (en) * | 2015-05-18 | 2015-09-02 | 青岛尚德生物技术有限公司 | Fermentation substance prepared by adding fish soluble in corn-soybean meal diet and fermentation method of fermentation substance |
| CN104909846A (en) * | 2015-05-18 | 2015-09-16 | 青岛根源生物技术集团有限公司 | Bacillus amyloliquefaciens HFJ-7-containing composite chicken manure decomposed starter and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103798540A (en) * | 2013-12-21 | 2014-05-21 | 河南旭瑞食品有限公司 | Multi-strain microorganism pig feed additive and production method thereof |
| CN103947827A (en) * | 2014-05-16 | 2014-07-30 | 丁友昉 | Fermentation agent and preparation method of probiotics and straw fermented feed |
-
2014
- 2014-11-03 CN CN201410608294.6A patent/CN104450599A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103798540A (en) * | 2013-12-21 | 2014-05-21 | 河南旭瑞食品有限公司 | Multi-strain microorganism pig feed additive and production method thereof |
| CN103947827A (en) * | 2014-05-16 | 2014-07-30 | 丁友昉 | Fermentation agent and preparation method of probiotics and straw fermented feed |
Non-Patent Citations (2)
| Title |
|---|
| 农业部: "《饲料添加剂品种目录（2013）》", 30 December 2013 * |
| 杨洁彬 等: "《乳酸菌－生物学基础及应用》", 30 April 1996, 中国轻工业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872379A (en) * | 2015-05-18 | 2015-09-02 | 青岛尚德生物技术有限公司 | Fermentation substance prepared by adding fish soluble in corn-soybean meal diet and fermentation method of fermentation substance |
| CN104909846A (en) * | 2015-05-18 | 2015-09-16 | 青岛根源生物技术集团有限公司 | Bacillus amyloliquefaciens HFJ-7-containing composite chicken manure decomposed starter and application thereof |
| CN104872379B (en) * | 2015-05-18 | 2018-07-24 | 青岛尚德生物技术有限公司 | A kind of fermentate and its fermentation process adding the molten slurry of fish in corn-soybean meal diet |
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