CN108611284A - A kind of microzyme culture medium for the steamed bun that ferments - Google Patents
A kind of microzyme culture medium for the steamed bun that ferments Download PDFInfo
- Publication number
- CN108611284A CN108611284A CN201611146305.9A CN201611146305A CN108611284A CN 108611284 A CN108611284 A CN 108611284A CN 201611146305 A CN201611146305 A CN 201611146305A CN 108611284 A CN108611284 A CN 108611284A
- Authority
- CN
- China
- Prior art keywords
- triangular flask
- culture
- steamed bun
- culture medium
- ferments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000001963 growth medium Substances 0.000 title claims abstract description 11
- 235000013312 flour Nutrition 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 229920001817 Agar Polymers 0.000 claims abstract description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 7
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 7
- 240000003183 Manihot esculenta Species 0.000 claims abstract description 7
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims abstract description 7
- 240000008042 Zea mays Species 0.000 claims abstract description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 7
- 239000008272 agar Substances 0.000 claims abstract description 7
- 235000005822 corn Nutrition 0.000 claims abstract description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims abstract description 7
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims abstract description 7
- 235000019797 dipotassium phosphate Nutrition 0.000 claims abstract description 7
- 239000008103 glucose Substances 0.000 claims abstract description 7
- 239000008101 lactose Substances 0.000 claims abstract description 7
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims abstract description 4
- 235000015278 beef Nutrition 0.000 claims abstract description 4
- 229940099352 cholate Drugs 0.000 claims abstract description 4
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 claims abstract description 4
- 238000004090 dissolution Methods 0.000 claims abstract description 4
- 230000000694 effects Effects 0.000 claims abstract description 4
- 239000011159 matrix material Substances 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 235000020997 lean meat Nutrition 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 238000007711 solidification Methods 0.000 claims description 3
- 230000008023 solidification Effects 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 3
- 241000235342 Saccharomycetes Species 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention is a kind of microzyme culture medium for the steamed bun that ferments, and raw material proportioning is following (g/L)：Corn flour 3.3 4.4, tapioca flour 1.2 2.0, powdered beef 2.8 3.5, yeast powder 1.2 1.8, potassium hydrogen phosphate 1.5 2.0,1 1.5 glucose 2.2 3.0 of Pig cholate, lactose 2.3 2.5, agar 25 30, pH value 7 ± 0.2, temperature are 24 ± 1 DEG C；It uses triangular flask as culture tool, chooses the triangular flask of 350ml, will pour into triangular flask after raw material proportioning, with the water dissolution of 100ml, then shake up, sterilized triangular flask to be placed in sterilization box；After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high saccharomycete of production activity.The culture medium of the present invention prepares fairly simple, and culture medium nutrition is more abundant, the growth characteristics of entirely appropriate saccharomycete.Saccharomycete after culture is for the steamed bun that ferments, and steamed bun is soft, and rich in toughness, and mouthfeel is very good.
Description
Technical field
The present invention relates to saccharomycete culture technique field, specifically a kind of microzyme culture medium for the steamed bun that ferments.
Background technology
Saccharomycete is the main bacteria seed of fermentation, and Spawn incubation obtains good ring, directly influences the quality of fermentation.Therefore, it carries out
Cultivation work is very important, however present culture medium is chemical solution mostly, pollutes environment, has chemistry residual after culture
It stays, influences strain development.
Invention content
The present invention provides a kind of microzyme culture medium for the steamed bun that ferments.
The technical solution adopted by the present invention is：
Raw material proportioning is following (g/L)：Corn flour 3.3-4.4, tapioca flour 1.2-2.0, powdered beef 2.8-3.5, yeast powder
1.2-1.8, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-2.5, agar 25-30, pH value 7
± 0.2, temperature is 24 ± 1 DEG C；
It uses triangular flask as culture tool, chooses the triangular flask of 350ml, will be poured into triangular flask after raw material proportioning, used
The water dissolution of 100ml, then shakes up, and is sterilized triangular flask to be placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and yeast powder provide nitrogen source, vitamin
And growth factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high ferment of production activity
Female bacterium.
It is an advantage of the invention that：Above-mentioned culture medium prepares fairly simple, and culture medium nutrition is more abundant, entirely appropriate yeast
The growth characteristics of bacterium.Saccharomycete after culture is for the steamed bun that ferments, and steamed bun is soft, and rich in toughness, and mouthfeel is very good.
Specific implementation mode
Raw material proportioning is following (g/L)：Corn flour 3.3-4.4, tapioca flour 1.2-2.0, powdered beef 2.8-3.5, yeast powder
1.2-1.8, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-2.5, agar 25-30, pH value 7
± 0.2, temperature is 24 ± 1 DEG C；
It uses triangular flask as culture tool, chooses the triangular flask of 350ml, will be poured into triangular flask after raw material proportioning, used
The water dissolution of 100ml, then shakes up, and is sterilized triangular flask to be placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and yeast powder provide nitrogen source, vitamin
And growth factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high ferment of production activity
Female bacterium.
Claims (1)
1. a kind of microzyme culture medium for the steamed bun that ferments, it is characterised in that：
Raw material proportioning is following (g/L)：Corn flour 3.3-4.4, tapioca flour 1.2-2.0, powdered beef 2.8-3.5, yeast powder 1.2-
1.8, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-2.5, agar 25-30, pH value 7 ±
0.2, temperature is 24 ± 1 DEG C；
It uses triangular flask as culture tool, chooses the triangular flask of 350ml, will be poured into triangular flask after raw material proportioning, with 100ml's
Then water dissolution shakes up, sterilized triangular flask to be placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and yeast powder provide nitrogen source, vitamin and life
The long factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high yeast of production activity
Bacterium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611146305.9A CN108611284A (en) | 2016-12-13 | 2016-12-13 | A kind of microzyme culture medium for the steamed bun that ferments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611146305.9A CN108611284A (en) | 2016-12-13 | 2016-12-13 | A kind of microzyme culture medium for the steamed bun that ferments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108611284A true CN108611284A (en) | 2018-10-02 |
Family
ID=63657837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611146305.9A Pending CN108611284A (en) | 2016-12-13 | 2016-12-13 | A kind of microzyme culture medium for the steamed bun that ferments |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108611284A (en) |
-
2016
- 2016-12-13 CN CN201611146305.9A patent/CN108611284A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181002 |