CN107699504A - A kind of Bradley yeast culture medium and preparation method thereof - Google Patents
A kind of Bradley yeast culture medium and preparation method thereof Download PDFInfo
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- CN107699504A CN107699504A CN201711217443.6A CN201711217443A CN107699504A CN 107699504 A CN107699504 A CN 107699504A CN 201711217443 A CN201711217443 A CN 201711217443A CN 107699504 A CN107699504 A CN 107699504A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The invention discloses a kind of Bradley yeast culture medium and preparation method thereof, it is made up of the raw material of following weight percentage：Glucose 3% ~ 5%, potassium nitrate 1% ~ 2%, peptone 3.5% ~ 4.5%, corn steep liquor 1.5% ~ 2.5%, yeast nutritire salt 0.2% ~ 0.3%, potassium dihydrogen phosphate 0.05% ~ 0.1%, magnesium sulfate 0.05% ~ 0.1%.Bradley yeast culture medium cost provided by the invention is cheap, rapidly and efficiently, safety and stability; the fast-growth of saccharomyces boulardii can be effectively facilitated; the yield and viable bacteria number of saccharomyces boulardii are improved, and its preparation method technique is simple, raw material easily takes, easy to operation and large-scale production；And yeast nutritire salt and corn steep liquor in raw material, abundant inorganic salts and growth factor are provided for the growth fermentation of Bradley yeast, substantially reduce saccharomyces boulardii cultivation cycle, in addition, culture medium is by the way of inorganic nitrogen-sourced and organic nitrogen source is combined, more lasting rapidly it can be played a role in the growth fermentation process of saccharomyces boulardii, effect is more preferable, has further speeded up the growth rate and viable count of saccharomyces boulardii.
Description
【Technical field】
The invention belongs to microbial technology field, and in particular to a kind of Bradley yeast culture medium and preparation method thereof.
【Background technology】
Saccharomyces boulardii containing abundant protein, amino acid, vitamin and various disappears as a kind of bacterium beneficial to human body
Change the nutriments such as enzyme, be often used cooperatively with most antibiotic, applied to fields such as resistance humans and animals diarrhoea.It can not only have
Effect reduces the usage amount of antibiotic, reduces its side effect, and helps to adjust functions of intestines and stomach, strengthens alimentary canal defence capability.
With the development and the improvement of people's living standards of society, people are also more and more to the demand of saccharomyces boulardii, therefore, how
Improve viable bacteria number in the culture efficiency and incubation of saccharomyces boulardii has turned into the emphasis of people's research.
It is well known that culture medium be it is a kind of manually prepare with maintain bacterial micro-organism and animal vegetable tissue grow needed for
Nutriment, it typically contains the nutriments such as carbohydrate, nitrogen substance, inorganic salts and vitamin, growth factor and water.People
The growth rates of bacterial micro-organism and animals and plants can be controlled by the regulation to medium component, therefore its composition is for thin
The fermentation and production of bacteria microorganism have vital effect.But make a general survey of the saccharomyces boulardii generally used both at home and abroad at present
Culture medium, the defects of incubation time is longer, low yield and positive rate be not high often be present, this had both been unfavorable for industrial operation and big rule
Mould produces, and also strongly limit its application in the field such as biological medicine and food processing, while also virtually add its life
Produce cost.Therefore, research and develop a kind of cost it is cheap, rapidly and efficiently, the Bradley yeast culture medium of safety and stability be imperative.
【The content of the invention】
The problem to be solved in the present invention is the deficiency for more than, there is provided a kind of cost is cheap, rapidly and efficiently, safety and stability, be easy to
Bradley yeast culture medium of operation and large-scale production and preparation method thereof.
The technical solution adopted by the present invention is as follows：A kind of Bradley yeast culture medium, weight percentage, its each raw material
Form and be：Glucose 3% ~ 5%, potassium nitrate 1% ~ 2%, peptone 3.5% ~ 4.5%, corn steep liquor 1.5% ~ 2.5%, yeast nutritire salt 0.2%
~ 0.3%, potassium dihydrogen phosphate 0.05% ~ 0.1%, magnesium sulfate 0.05% ~ 0.1%.
A kind of Bradley yeast culture medium preparation method, comprises the following steps：
（1）Glucose, potassium nitrate, peptone, corn steep liquor, yeast nutritire salt, potassium dihydrogen phosphate, magnesium sulfate each component raw material is molten
Obtained fluid nutrient medium is mixed evenly in solution in deionized water；
（2）The pH value of watery hydrochloric acid regulation fluid nutrient medium is added into above-mentioned gained fluid nutrient medium；
（3）The fluid nutrient medium for regulating pH value is put into sterilization treatment in full-automatic high-pressure autoclave Bradley yeast culture is made
Base finished product.
Specifically, the step（2）The scope of middle pH value is 5.0 ~ 5.5.
Specifically, the step（3）Middle sterilization treatment temperature is 115 °C, and the sterilization treatment time is 15 ~ 20min.
It is an advantage of the invention that：
1. Bradley yeast culture medium cost provided by the invention is cheap, rapidly and efficiently, safety and stability, Bradley ferment can be effectively facilitated
The fast-growth of female bacterium, the yield and viable bacteria number of saccharomyces boulardii are improved, and its preparation method technique is simple, raw material easily takes,
Easy to operation and large-scale production.
2. present invention selection potassium nitrate, corn steep liquor, yeast nutritire salt etc. are raw material, wide material sources, it is easily obtained, and wherein
Yeast nutritire salt and corn steep liquor, for Bradley yeast growth fermentation provide abundant inorganic salts and growth factor, contract significantly
Short saccharomyces boulardii cultivation cycle.
3. Bradley yeast culture medium provided by the invention, inorganic nitrogen-sourced relative to traditional single nitrogen source medium, utilization
The training method being combined with organic nitrogen source, being capable of more lasting performance rapidly in the growth fermentation process of saccharomyces boulardii
Effect, effect is more preferable, so as to further speed up the growth rate of saccharomyces boulardii and viable count, shortens saccharomyces boulardii training
The cycle is supported, is more beneficial for the popularization and application of saccharomyces boulardii.
【Embodiment】
In order to more fully understand the technology contents of the present invention, enter traveling one to technical solution of the present invention below by specific embodiment
Step is introduced and explanation.Following examples are descriptive, are not limited, it is impossible to the protection model of the present invention is limited with this
Enclose.
Embodiment 1
A kind of Bradley yeast culture medium, weight percentage, its each raw material composition are：Glucose 3%, potassium nitrate 1%, albumen
Peptone 3.5%, corn steep liquor 1.5%, yeast nutritire salt 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%.
A kind of Bradley yeast culture medium preparation method, comprises the following steps：
（1）Glucose, potassium nitrate, peptone, corn steep liquor, yeast nutritire salt, potassium dihydrogen phosphate, magnesium sulfate each component raw material is molten
Obtained fluid nutrient medium is mixed evenly in solution in deionized water；
（2）The pH value of watery hydrochloric acid regulation fluid nutrient medium is added into above-mentioned gained fluid nutrient medium to 5.0；
（3）The fluid nutrient medium for regulating pH value is put into sterilization treatment 15min in 115 °C of full-automatic high-pressure autoclave to be made
Bradley yeast culture medium finished product.
Embodiment two
A kind of Bradley yeast culture medium, weight percentage, its each raw material composition are：Glucose 4%, potassium nitrate 1.5%, egg
The white % of peptone 4, corn steep liquor 2%, yeast nutritire salt 0.25%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.08%.
A kind of Bradley yeast culture medium preparation method, comprises the following steps：
（1）Glucose, potassium nitrate, peptone, corn steep liquor, yeast nutritire salt, potassium dihydrogen phosphate, magnesium sulfate each component raw material is molten
Obtained fluid nutrient medium is mixed evenly in solution in deionized water；
（2）The pH value of watery hydrochloric acid regulation fluid nutrient medium is added into above-mentioned gained fluid nutrient medium to 5.2；
（3）The fluid nutrient medium for regulating pH value is put into sterilization treatment 18min in 115 °C of full-automatic high-pressure autoclave to be made
Bradley yeast culture medium finished product.
Embodiment three
A kind of Bradley yeast culture medium, weight percentage, its each raw material composition are：Glucose 5%, potassium nitrate 2%, albumen
Peptone 4.5%, corn steep liquor 2.5%, yeast nutritire salt 0.3%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%.
A kind of Bradley yeast culture medium preparation method, comprises the following steps：
（1）Glucose, potassium nitrate, peptone, corn steep liquor, yeast nutritire salt, potassium dihydrogen phosphate, magnesium sulfate each component raw material is molten
Obtained fluid nutrient medium is mixed evenly in solution in deionized water；
（2）The pH value of watery hydrochloric acid regulation fluid nutrient medium is added into above-mentioned gained fluid nutrient medium to 5.5；
（3）The fluid nutrient medium for regulating pH value is put into sterilization treatment 20min in 115 °C of full-automatic high-pressure autoclave to be made
Bradley yeast culture medium finished product.
Embodiment described above only expresses embodiments of the present invention, and its description is more specific and detailed, but can not
Therefore it is interpreted as the limitation to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art,
On the premise of this design is not departed from, some improvement can also be made, these belong to protection scope of the present invention.Therefore, originally
The protection domain of patent of invention should be determined by the appended claims.
Claims (4)
- A kind of 1. Bradley yeast culture medium, it is characterised in that：Weight percentage, its each raw material composition are：Glucose 3% ~ 5%th, potassium nitrate 1% ~ 2%, peptone 3.5% ~ 4.5%, corn steep liquor 1.5% ~ 2.5%, yeast nutritire salt 0.2% ~ 0.3%, potassium dihydrogen phosphate 0.05% ~ 0.1%, magnesium sulfate 0.05% ~ 0.1%.
- 2. the preparation method of Bradley yeast culture medium as claimed in claim 1, it is characterised in that comprise the following steps：（1）Glucose, potassium nitrate, peptone, corn steep liquor, yeast nutritire salt, potassium dihydrogen phosphate, magnesium sulfate each component raw material is molten Obtained fluid nutrient medium is mixed evenly in Xie Yushui；（2）The pH value of watery hydrochloric acid regulation fluid nutrient medium is added into above-mentioned gained fluid nutrient medium；（3）By the fluid nutrient medium for regulating pH value be put into high-pressure sterilizing pot sterilization treatment be made Bradley yeast culture medium into Product.
- 3. the preparation method of Bradley yeast culture medium as claimed in claim 2, it is characterised in that：The step（2）Middle pH value Scope be 5.0 ~ 5.5.
- 4. the preparation method of Bradley yeast culture medium as claimed in claim 2, it is characterised in that：The step（3）Middle sterilizing Treatment temperature is 115 °C, and the sterilization treatment time is 15 ~ 20min.
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| CN201711217443.6A CN107699504A (en) | 2017-11-28 | 2017-11-28 | A kind of Bradley yeast culture medium and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110577903A (en) * | 2019-10-08 | 2019-12-17 | 东北农业大学 | Cryptococcus albidus fermentation medium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550826A (en) * | 2012-02-07 | 2012-07-11 | 山东盛泰生物科技有限公司 | Active dry saccharomyces boulardii feed additive and its preparation method and use |
| CN103652320A (en) * | 2012-09-17 | 2014-03-26 | 中国农业科学院饲料研究所 | Preparation method of active microbial agent of forage anti-diarrhea yeast |
-
2017
- 2017-11-28 CN CN201711217443.6A patent/CN107699504A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550826A (en) * | 2012-02-07 | 2012-07-11 | 山东盛泰生物科技有限公司 | Active dry saccharomyces boulardii feed additive and its preparation method and use |
| CN103652320A (en) * | 2012-09-17 | 2014-03-26 | 中国农业科学院饲料研究所 | Preparation method of active microbial agent of forage anti-diarrhea yeast |
Non-Patent Citations (4)
| Title |
|---|
| 刘敬为: "布拉酵母的发酵工艺及其在玉米生产上的应用", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 房贤坤等: "布拉酵母高密度培养条件的研究", 《安徽农业科学》 * |
| 李秀婷: "《现代啤酒生产工艺》", 30 June 2013, 中国农业大学出版社 * |
| 杜晓蒙等: "培养基组成对布拉氏酵母生长的影响及其优化", 《食品研究与开发》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110577903A (en) * | 2019-10-08 | 2019-12-17 | 东北农业大学 | Cryptococcus albidus fermentation medium |
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Application publication date: 20180216 |