CN108220178A - A kind of culture medium for cultivating Pseudomonas aeruginosa - Google Patents
A kind of culture medium for cultivating Pseudomonas aeruginosa Download PDFInfo
- Publication number
- CN108220178A CN108220178A CN201611137803.7A CN201611137803A CN108220178A CN 108220178 A CN108220178 A CN 108220178A CN 201611137803 A CN201611137803 A CN 201611137803A CN 108220178 A CN108220178 A CN 108220178A
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- Prior art keywords
- triangular flask
- culture medium
- culture
- glucose
- pseudomonas aeruginosa
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
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- General Engineering & Computer Science (AREA)
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Abstract
The present invention is a kind of culture medium for cultivating Pseudomonas aeruginosa, and raw material proportioning is following (g/L)：Sugar-cane juice 4.5 6.5, corn flour 3.3 4.4, tapioca flour 1.2 2.0, beef extract 5 10, ammonium citrate 25, potassium hydrogen phosphate 1.5 2.0,1 1.5 glucose 2.2 3.0 of Pig cholate, lactose 2.3 2.5, agar 25 30, pH value 7 ± 0.2, temperature are 24 ± 1 DEG C.The culture medium of the present invention prepares fairly simple, and culture medium nutrition is more abundant.
Description
Technical field
The present invention relates to saccharomycete culture technique field, specifically a kind of culture medium for cultivating Pseudomonas aeruginosa.
Background technology
The bacillus that a kind of pathogenicity is relatively low but drug resistance is strong.Nature is widely present in, is one of wound infection more typically
Kind bacterium.
Invention content
The present invention provides a kind of culture medium for cultivating Pseudomonas aeruginosa.
The technical solution adopted by the present invention is：
Raw material proportioning is following (g/L)：Sugar-cane juice 4.5-6.5, corn flour 3.3-4.4, tapioca flour 1.2-2.0, beef extract 5-
10, ammonium citrate 2-5, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-2.5, agar 25-
30, pH value 7 ± 0.2, temperature is 24 ± 1 DEG C；
By the use of triangular flask as culture tool, the triangular flask of 350ml is chosen, will be poured into triangular flask after raw material proportioning, is used
The water dissolution of 100ml, then shakes up, and is sterilized triangular flask is placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and dusty yeast provide nitrogen source, vitamin
And growth factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high ferment of production activity
Female bacterium.
It is an advantage of the invention that：The culture medium of the present invention prepares fairly simple, and culture medium nutrition is more abundant.
Specific embodiment
Raw material proportioning is following (g/L)：Sugar-cane juice 4.5-6.5, corn flour 3.3-4.4, tapioca flour 1.2-2.0, beef extract 5-
10, ammonium citrate 2-5, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-2.5, agar 25-
30, pH value 7 ± 0.2, temperature is 24 ± 1 DEG C；
By the use of triangular flask as culture tool, the triangular flask of 350ml is chosen, will be poured into triangular flask after raw material proportioning, is used
The water dissolution of 100ml, then shakes up, and is sterilized triangular flask is placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and dusty yeast provide nitrogen source, vitamin
And growth factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high ferment of production activity
Female bacterium.
Claims (1)
1. a kind of culture medium for cultivating Pseudomonas aeruginosa, it is characterised in that：
Raw material proportioning is following (g/L)：Sugar-cane juice 4.5-6.5, corn flour 3.3-4.4, tapioca flour 1.2-2.0, beef extract 5-10, lemon
Lemon acid ammonium 2-5, potassium hydrogen phosphate 1.5-2.0, Pig cholate 1-1.5 glucose 2.2-3.0, lactose 2.3-2.5, agar 25-30, pH value
7 ± 0.2, temperature is 24 ± 1 DEG C；
By the use of triangular flask as culture tool, the triangular flask of 350ml is chosen, will be poured into triangular flask after raw material proportioning, with 100ml's
Then water dissolution shakes up, sterilized triangular flask is placed in sterilization box；
Corn flour, tapioca flour provide the nutriments such as carbon source, protein；Lean meat powder and dusty yeast provide nitrogen source, vitamin and life
The long factor；Glucose and lactose provide carbon source for fermentable saccharide；Potassium hydrogen phosphate is buffer；Use agar solidification；
After the culture matrix manufacturing, strain is accessed, is put into culture bottle and further cultivates, the high yeast of production activity
Bacterium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611137803.7A CN108220178A (en) | 2016-12-12 | 2016-12-12 | A kind of culture medium for cultivating Pseudomonas aeruginosa |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611137803.7A CN108220178A (en) | 2016-12-12 | 2016-12-12 | A kind of culture medium for cultivating Pseudomonas aeruginosa |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108220178A true CN108220178A (en) | 2018-06-29 |
Family
ID=62638618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611137803.7A Pending CN108220178A (en) | 2016-12-12 | 2016-12-12 | A kind of culture medium for cultivating Pseudomonas aeruginosa |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108220178A (en) |
-
2016
- 2016-12-12 CN CN201611137803.7A patent/CN108220178A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180629 |
|
| WD01 | Invention patent application deemed withdrawn after publication |