CN108210975B - Collagen Dressing for Acne Treatment - Google Patents

Abstract

The present invention relates to collagen dressings for the treatment of acne. The collagen dressing of the present invention includes an auxiliary liquid and a dressing base, wherein the dressing liquid is mainly composed of 1-10 parts of marine hydrolyzed collagen, 0.5-4 parts of dipotassium glycyrrhizinate, 0.2-1 part of aloe vera gel powder and 1000 parts of It is prepared from water, and the substrate is a non-woven fabric, preferably a chitosan non-woven fabric. The collagen dressing of the present invention is mainly made of pure natural materials, is simple and safe, has good tissue compatibility, and is non-irritating, has good antibacterial and pigmentation reduction effects caused by acne, and has good acne treatment and repair effects.

Description

Collagen Dressing for Acne Treatment

technical field

The present invention relates to the field of dressings, in particular to collagen dressings for treating acne.

Background technique

Acne (Acne vulgaris), also known as acne (Acne), is a common skin disease, 80% of people between the ages of 11 and 30 suffer from this disease, mostly on the face, back, chest, etc. Areas containing sebaceous glands. Acne can persist for years, causing disfigurement or permanent scarring and having a severe negative impact on the patient's psychological growth.

Etiological analysis suggests that the severity of acne is related to epidermal Staphylococcus aureus and subcutaneous Propionibacterium acnes, and how to effectively inhibit and kill these two bacteria is the key to acne treatment.

Modern acne treatment is mainly based on oral antibiotics, hormones, retinoic acid drugs and topical anti-inflammatory drugs. The course of treatment is relatively long, the disease is easy to recur, and it is easy to produce adverse reactions, and it is easy to form scars or pigmentation. Therefore, the development of a safer, gentler, longer-lasting and more comprehensive product has become a popular choice.

In view of this, the present invention is proposed.

SUMMARY OF THE INVENTION

The first object of the present invention is to provide a dressing liquid for treating acne, which is mainly made of pure natural materials, is simple and safe, has good tissue compatibility, is non-irritating, has good bacteriostasis and reduces acne caused by acne. Pigmentation effect, has a good treatment and repair effect on acne.

The second object of the present invention is to provide a dressing for treating acne, the dressing comprising the aforementioned dressing base and the aforementioned dressing liquid, which is convenient to use and has good therapeutic and repairing effects on acne.

In order to realize the above-mentioned purpose of the present invention, the following technical solutions are specially adopted:

A dressing liquid for treating acne, in parts by weight, the dressing liquid is mainly prepared from 1-10 parts of marine hydrolyzed collagen, 0.5-4 parts of dipotassium glycyrrhizinate, 0.2-1 part of aloe vera gel powder and 1000 parts of water made.

The preparation raw materials of the dressing liquid of the present invention include marine hydrolyzed collagen, dipotassium glycyrrhizate and aloe vera gel powder. Among them, as a skin conditioning agent, dipotassium glycyrrhizate has the functions of antibacterial, anti-inflammatory and calming, reducing redness and promoting healing. After conditioning with dipotassium glycyrrhizate, it can relieve the microbial infection and inflammation of the facial skin of acne patients as a whole, and improve the facial skin. The overall physiological condition of the skin makes the skin in a physiological state with strong absorption and repair ability, which is conducive to the subsequent penetration and effect of marine hydrolyzed collagen and aloe vera gel. Has a good inhibitory effect. Aloe vera gel contains a large amount of phenols, polysaccharides and amino acids, which has a good inhibitory effect on Staphylococcus aureus, Escherichia coli and subcutaneous Propionibacterium acnes in vitro, and aloe vera polysaccharides and vitamins have good moisturizing and moisturizing effect on the skin. whitening effect. Therefore, aloe vera gel has a targeted inhibitory effect on the bacteria that cause acne, which can cure both the symptoms and symptoms. At the same time, its whitening and moisturizing effects are also of great benefit to the repair of the skin. The marine hydrolyzed collagen can penetrate into the stratum corneum and dermis, and its tyrosine residues can compete with tyrosine in the skin, inhibiting the conversion of tyrosine into dopaquinone by tyrosinase catalyzed by tyrosinase, preventing to a certain extent The formation of melanin, reducing pigmentation. Furthermore, marine hydrolyzed collagen, dipotassium glycyrrhizate, and aloe vera gel powder are all natural extracts with good histocompatibility, no irritation to the skin, and are safe and reliable.

To sum up, the dipotassium glycyrrhizate, aloe vera gel and marine hydrolyzed collagen in the dressing liquid of the present invention have the functions of mutual coordination and synergy on the whole, wherein the conditioning effect of dipotassium glycyrrhizate can be from Overall, it improves the skin condition of patients and enhances the skin's absorption and utilization of aloe vera gel and marine hydrolyzed protein. The inhibitory effect of aloe vera gel on bacteria can further enhance the conditioning effect of dipotassium glycyrrhizinate on the skin. At the same time, its good water retention, Facilitates skin absorption of potassium glycyrrhizinate and marine hydrolyzed collagen. The marine hydrolyzed collagen can not only inhibit the deposition of pigment, but also provide the amino acids for skin metabolism, improve the living environment of skin cells, enhance the vitality and resistance of the skin, and assist dipotassium glycyrrhizate and aloe vera gel in conditioning ,Therapeutic effect.

In some specific embodiments, the marine hydrolyzed collagen is 1 part, 5 parts or 10 parts by weight.

In some specific embodiments, the dipotassium glycyrrhizinate is 0.5 parts, 2 parts, or 4 parts.

In some specific embodiments, the aloe vera gel powder is 0.2 part, 0.5 part or 1 part.

In some specific embodiments, the molecular weight of the marine hydrolyzed collagen is 400-1500 Daltons, for example, 400 Daltons, 500 Daltons, 600 Daltons, 700 Daltons, 800 Daltons , 900 Daltons, 1000 Daltons, 1100 Daltons, 1200 Daltons, 1300 Daltons or 1400 Daltons.

In some specific embodiments, the polymer dispersity index (PDI) of the marine hydrolyzed collagen is ≤3.0, preferably, PDI≤2.4, more preferably, PDI≤2.0.

In some specific embodiments, the marine hydrolyzed collagen is selected from Nippi hydrolyzed collagen, Rousselot hydrolyzed collagen, Oriental marine hydrolyzed collagen or Shandong Tianjiu hydrolyzed collagen.

The present invention also unexpectedly found that the molecular weight and PDI value affect the inhibitory effect of marine hydrolyzed collagen on tyrosinase. Therefore, the method of the present invention also limits the molecular weight and PDI value of the marine hydrolyzed protein, and the marine hydrolyzed protein within the aforementioned scope of the present invention has a good tyrosinase inhibitory effect.

In some specific embodiments, in order to be suitable for use on human skin, the pH value of the dressing solution of the present invention is 4.0-6.0, preferably 5.0-6.0.

In some specific embodiments, the aloe vera gel powder is aloe vera gel powder.

The present invention also relates to a dressing for the treatment of acne comprising a dressing base and the aforementioned dressing solution.

In some specific embodiments, the dressing substrate is a non-woven fabric, preferably, the non-woven fabric is a chitosan non-woven fabric; more preferably, the mass fraction of chitosan in the non-woven fabric is ≥ 20%, or ≥ 30%, or ≥ 50%, or = 100%.

The substrate of the aforementioned dressing of the present invention is a non-woven fabric containing chitosan, wherein the chitosan has excellent antibacterial activity, and has a good bacteriostatic effect on common strains such as Staphylococcus aureus, Escherichia coli, Lactobacillus, etc. The therapeutic effect of the dressing on acne can be further enhanced.

In some specific embodiments, the dressing base is soaked in the dressing solution, or the dressing base and the dressing solution are packaged separately.

Compared with the prior art, the beneficial effects of the present invention are:

(1) Dressing liquid of the present invention, dressing are through the compatibility of dipotassium glycyrrhizate, aloe vera gel active substance, marine hydrolyzed collagen polypeptide and/or chitosan, and have the functions of killing the main pathogenic bacteria of acne, conditioning the skin and inhibiting pigmentation. The effect of deposition has a good therapeutic and repairing effect on acne as a whole.

(2) The dressing liquid and dressing of the present invention are mainly made of pure natural materials, and have the advantages of simplicity and safety, good tissue compatibility, and no irritation to the skin.

(3) The present invention further defines the molecular weight and PDI value of marine hydrolyzed collagen, which can further enhance the inhibitory effect of the dressing solution and dressing on tyrosinase activity.

Description of drawings

In order to illustrate the specific embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the specific embodiments or the prior art. Obviously, the accompanying drawings in the following description The drawings are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative efforts.

Fig. 1 is the skin condition of the subject's right face after the treatment;

Figure 2 shows the skin condition of the subject's left face after the end of the treatment.

Detailed ways

The embodiments of the present invention will be described in detail below with reference to the examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. If the specific conditions are not indicated in the examples, it is carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used without the manufacturer's indication are conventional products that can be purchased in the market.

Example 1

A dressing for treating acne, comprising a dressing liquid and a dressing base, wherein, in parts by weight, the dressing liquid is mainly composed of 1 part of marine hydrolyzed collagen, 0.5 part of dipotassium glycyrrhizinate, 0.2 part of aloe vera gel powder and 1000 parts of water; the dressing base is chitosan non-woven fabric, wherein the mass fraction of chitosan is 20%.

The marine hydrolyzed collagen is Nippi hydrolyzed collagen, and the aloe vera gel powder is aloe vera gel powder.

The specific preparation method of the auxiliary material includes: 1. In a sterile environment, the marine hydrolyzed collagen, dipotassium glycyrrhizate and aloe vera gel powder are added into purified water, and the auxiliary material solution is obtained by stirring and dissolving. 2. In a sterile environment, mix the aforementioned adjuvant liquid with chitosan non-woven fabric, package, and then sterilize by irradiation.

Example 2

The dressing was prepared with reference to the method described in Example 1, except that the dressing solution was mainly prepared from 5 parts of marine hydrolyzed collagen, 2 parts of dipotassium glycyrrhizinate, 1 part of aloe vera gel powder and 1000 parts of water; The mass fraction of chitosan in the non-woven fabric is 30%.

Example 3

The dressing was prepared with reference to the method described in Example 1, except that the dressing solution was mainly prepared from 10 parts of marine hydrolyzed collagen, 4 parts of dipotassium glycyrrhizinate, 1 part of aloe vera gel powder and 1000 parts of water; The mass fraction of chitosan in the non-woven fabric is 100%.

Example 4

The dressing was prepared with reference to the method described in Example 2, except that the mass fraction of chitosan in the non-woven fabric was 50%.

Example 5

The dressing was prepared according to the method described in Example 2, except that the mass fraction of chitosan in the non-woven fabric was 100%.

Example 6

The dressing was prepared with reference to the method described in Example 1, except that the marine hydrolyzed collagen was Rousselot marine hydrolyzed collagen.

Example 7

The dressing was prepared with reference to the method described in Example 1, except that the marine hydrolyzed collagen was oriental marine hydrolyzed collagen.

Example 8

The dressing was prepared with reference to the method described in Example 1, the only difference being that the marine hydrolyzed collagen was Shandong Changchang marine hydrolyzed collagen.

Example 9

The dressing was prepared with reference to the method described in Example 1, the only difference being that, during the preparation process, the dressing liquid and the dressing base were packaged and sterilized independently.

Experimental example 1

The physicochemical properties and tyrosinase inhibition rate of the marine hydrolyzed collagen used in the foregoing examples were detected, wherein the specific detection methods and detection results are shown below.

1. High-efficiency size exclusion chromatography was used to detect the molecular weight and polymer dispersibility index (PDI) of different brands of marine hydrolyzed collagen (Nippi, Rousselot, Oriental Ocean and Shandong Tianjiu). The specific test results are shown in Table 1. shown.

Table 1 Physicochemical properties of different marine hydrolyzed collagens

2. The tyrosinase inhibition rate of different brands of marine hydrolyzed collagen was detected by the mushroom tyrosinase dopa rate oxidation method. The specific detection methods and results are shown below.

1) Sample processing: Use ultrapure water to prepare a 10g/L solution of different brands of marine hydrolyzed collagen (tyrosinase is copper protein, and the solution should not contain copper ions) as a sample solution for later use.

2) Experimental operation: carry out the biochemical reaction in a glass tube, add the required pH6.8 phosphate buffer (6.8g dipotassium hydrogen phosphate and 0.944g sodium hydroxide to 1000ml), sample solution, and kojic acid solution to each tube. (10g/L) and enzyme solution (about 3.5mg tyrosinase to 100ml) were reacted in a water bath at 37°C for 10min, and then L-dopa solution (2.5g/L) was added in turn, and the reaction was performed in a water bath at 37°C 10min, measure the absorbance at 475nm wavelength. The experimental system design is shown in Table 2.

Table 2 Experimental system design table

3) Calculation method of inhibition rate: The inhibition strength of samples to tyrosinase activity is expressed as inhibition rate. Sample inhibition rate=[positive control absorbance value-(sample tube absorbance value-negative control absorbance value)]/positive control absorbance value×100%, standard control inhibition rate=(positive control absorbance value-standard tube absorbance value)/positive control Absorbance value × 100%, high inhibition rate indicates that it has the highest inhibitory intensity on enzyme activity. Standard reference: In order to ensure the accuracy of the experimental results, there should be a standard reference for testing in the laboratory. Kojic acid is selected as the standard control. In different batches of experiments, the inhibition rate of the kojic acid standard control will be different. The inhibition rate of the standard control can be converted into 100%, and the inhibition rate of the test substance is changed in equal proportions before making a comparison. . For example, according to the above formula, the inhibition rate of the test substance is 18%, the inhibition rate of the standard control substance is 90%, and the converted inhibition rate of the test substance is 18%/90%=20%.

Table 3 Tyrosinase inhibition rate of different brands of marine hydrolyzed collagen

2. Analysis of test results: According to the molecular weight and polymer dispersibility index of marine hydrolyzed collagen shown in Table 1 and the tyrosinase inhibition rate shown in Table 3, the tyrosinase inhibition rate of marine hydrolyzed collagen is related to its molecular weight, Among them, marine hydrolyzed collagen with molecular weight below 1000 Daltons and PDI index below 2.4 has a good inhibitory effect on tyrosinase.

Experimental example 2

Take the raw materials used in Example 5 and the finished dressing, and detect its cytotoxicity. The specific detection method refers to the national standard GB/T 16886.5-2003/ISO 10993-5:1999, and the specific detection results are shown in Table 4.

According to the relative growth rate (RGR) of the detected cells, the samples are classified for toxicity and eligibility. The specific grading standards are as follows:

Grade 0: RCG≥100%; Grade 1: 99%≥RCG≥75; Grade 2: 74≥RCG≥50; Grade 3: 49≥RCG≥25; Grade 4: 24≥RGR≥1; Grade 5=0.

Qualification criteria for samples: 0 to 1 grades are qualified; according to comprehensive analysis of cell morphology, 2 grades are qualified, and 3 to 5 grades are unqualified.

According to the experimental results described in Table 4, the raw materials and finished products involved in the present invention have no cytotoxicity and are safe and reliable.

Table 4 Cytotoxicity test results

Experimental example 3

The dressings described in Examples 1 to 5 were taken, and their inhibitory effects on Staphylococcus aureus (ATCC6538) and Propionibacterium acnes (ATCC11827) were tested. For specific detection methods, refer to the national standard GB/T 20944, and specific test results refer to Table 5. According to the test results shown in Table 5, the dressing of the present invention has a good bacteriostatic effect on Staphylococcus aureus and Propionibacterium acnes.

Table 5 Bacteriostatic test results

Experimental example 4

Two subjects were called to try the dressing described in Example 5, and the details were as follows:

1. Subject

Number: 2; Gender: Male; Age: 25-33 years old; Physiological condition: moderate acne on the face, perennial redness and swelling, and hard acne.

2. Test method

In the half-face experiment, 2 people used the dressing described in Example 4 of the present invention on the right half of the face, and the left half of the face was used as a control. The experimental period was 4 weeks. The dressing was used once a day for the first 10 days for 20-30 min/time, and then 2-3 times a week for 20-30 min/time.

3. Test results

After the test, 7-8 people were called to blindly evaluate the skin condition of the left and right faces of the two subjects. They all believed that the facial acne on the right side of the two subjects was significantly reduced, the scars were reduced, and the gloss was increased, and the overall skin condition was significantly better. on the left skin. The control results of the left and right facial skin of one of the subjects after the test are shown in Figures 1 to 2. According to the results shown in the figure, after the dressing treatment, the subject's acne condition on the right face was significantly improved .

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand: It is still possible to modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the embodiments of the present invention. range.

Claims (6)

1. a dressing for the treatment of acne, characterized in that the dressing is made up of a dressing base and a dressing liquid; The dressing liquid is prepared from 1-10 parts by weight of marine hydrolyzed collagen, 0.5-4 parts of dipotassium glycyrrhizate, 0.2-1 part of aloe vera gel powder and 1000 parts of water; The pH value of the dressing solution is 4.0-6.0; The molecular weight of the marine hydrolyzed collagen is 400-1500 Daltons; The polymer dispersity index (PDI) of the marine hydrolyzed collagen is less than or equal to 3.0; The marine hydrolyzed collagen is selected from Nippi hydrolyzed collagen, Rousselot hydrolyzed collagen, Oriental marine hydrolyzed collagen or Shandong Tianjiu hydrolyzed collagen; The aloe vera gel powder is Curacao aloe vera gel powder; The dressing base is a non-woven fabric; The non-woven fabric is chitosan non-woven fabric; The mass fraction of chitosan in the non-woven fabric is ≥20%; The dressing base and the dressing solution are packaged separately. 2. The dressing according to claim 1 is characterized in that, in parts by weight, the marine hydrolyzed collagen is 1 part, 5 parts or 10 parts; the dipotassium glycyrrhizinate is 0.5 part, 2 parts or 4 parts part; the aloe vera gel powder is 0.2 part, 0.5 part or 1 part. 3. dressing according to claim 1 is characterized in that, the molecular weight of described marine hydrolyzed collagen is 400 daltons, 500 daltons, 600 daltons, 700 daltons, 800 daltons, 900 daltons Daltons, 1000 Daltons, 1100 Daltons, 1200 Daltons, 1300 Daltons or 1400 Daltons. 4. The dressing according to claim 1, wherein the PDI of the marine hydrolyzed collagen is less than or equal to 2.4. 5 . The dressing according to claim 1 , wherein the PDI of the marine hydrolyzed collagen is less than or equal to 2.0. 6 . 6 . The dressing according to claim 1 , wherein the pH value of the dressing solution is 5.0-6.0. 7 .

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