CN108210975B

CN108210975B - Collagen dressing for treating acne

Info

Publication number: CN108210975B
Application number: CN201810119795.6A
Authority: CN
Inventors: 于德君; 李八方; 王园园; 代元坤; 宋文山
Original assignee: Qingdao Marine Biomedical Research Institute Co Ltd
Current assignee: Haisheng Health Technology Qingdao Co ltd; Qingdao Institute of marine biomedicine
Priority date: 2018-02-06
Filing date: 2018-02-06
Publication date: 2020-12-22
Anticipated expiration: 2038-02-06
Also published as: CN108210975A

Abstract

The present invention relates to a collagen dressing for the treatment of acne. The collagen dressing comprises an auxiliary material liquid and a dressing substrate, wherein the dressing liquid is mainly prepared from 1-10 parts of marine hydrolyzed collagen, 0.5-4 parts of dipotassium glycyrrhizinate, 0.2-1 part of aloe gel powder and 1000 parts of water, and the substrate is a non-woven fabric, preferably a chitosan non-woven fabric. The collagen dressing provided by the invention is mainly made of pure natural materials, is simple and safe, has good histocompatibility, is non-irritant, has good antibacterial and pigmentation reducing effects, and has good treatment and repair effects on acne.

Description

Collagen dressing for treating acne

Technical Field

The invention relates to the field of dressings, in particular to a collagen dressing for treating acne.

Background

Acne (Acne vulgaris), also called comedo (Acne), is a common skin disease, and 80% of people aged between 11 and 30 years suffer from the disease, and usually occur on parts rich in sebaceous glands, such as face, back and chest. Acne can persist for many years, resulting in disfigurement or permanent scarring and causing serious negative effects on the psychological development of the patient.

Etiological analysis suggests that the severity of acne is related to the presence of epidermal staphylococcus aureus and subcutaneous propionibacterium acnes, and how effectively to inhibit and kill these two bacteria is critical to the treatment of acne.

Modern acne treatment mainly adopts oral antibacterial drugs, hormones, tretinoin drugs and external anti-inflammatory drugs, has the problems of long treatment course, easy relapse of the state of an illness, easy generation of adverse reactions and the like, and is easy to form scars or pigmentation. Therefore, it is hopeful to develop a safer, milder, durable and overall-efficacy product.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The dressing liquid is mainly made of pure natural materials, is simple and safe, has good histocompatibility and no irritation, has good bacteriostatic action, reduces pigmentation caused by acne, and has good treatment and repair effects on the acne.

The second purpose of the invention is to provide a dressing for treating acne, which comprises the dressing substrate and the dressing liquid, is convenient to use, and has good treatment and repair effects on acne.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

the dressing liquid for treating acne is mainly prepared from 1-10 parts by weight of marine hydrolyzed collagen, 0.5-4 parts by weight of dipotassium glycyrrhizinate, 0.2-1 part by weight of aloe gel powder and 1000 parts by weight of water.

The preparation raw materials of the dressing liquid comprise marine hydrolyzed collagen, dipotassium glycyrrhizinate and aloe gel powder. The dipotassium glycyrrhizinate is taken as a skin conditioner, has the effects of inhibiting bacteria, diminishing inflammation, calming, reducing redness and swelling and promoting healing, can relieve microbial infection and inflammation of facial skin of acne patients on the whole by conditioning the dipotassium glycyrrhizinate, improves the overall physiological condition of the facial skin, enables the skin to be in a physiological state with strong absorption and repair capacity, is beneficial to the permeation and the effect of subsequent marine hydrolyzed collagen and aloe gel, can penetrate into the subcutaneous part in addition, and has a good inhibiting effect on propionibacterium acnes. The aloe gel contains a large amount of phenols, polysaccharides and amino acids, has good inhibitory effect on staphylococcus aureus, escherichia coli, subcutaneous propionibacterium acnes and the like in vitro, and has good moisturizing and whitening effects on skin by the aloe polysaccharides and vitamins. Therefore, the aloe gel has a targeted inhibiting effect on bacteria causing acne, can achieve the effect of treating both symptoms and root causes, and has great benefits on skin repair due to the whitening and moistening effects. The marine hydrolyzed collagen can permeate into the stratum corneum and the dermis, and the tyrosine residue of the marine hydrolyzed collagen can compete with tyrosine in the skin, inhibit the tyrosine from catalyzing and converting tyrosine into dopaquinone, prevent the formation of melanin to a certain extent and reduce the pigment deposition. In addition, the marine hydrolyzed collagen, the dipotassium glycyrrhizinate and the aloe gel powder are all pure natural extracts, have good histocompatibility, have no stimulation to skin, and are safe and reliable.

In conclusion, the dipotassium glycyrrhizinate, the aloe gel and the marine hydrolyzed collagen in the dressing liquid have the effects of mutual matching and synergy on the whole, wherein the conditioning effect of the dipotassium glycyrrhizinate can improve the skin state of a patient on the whole, the absorption and utilization of skin on the aloe gel and the marine hydrolyzed collagen are enhanced, the inhibition effect of the aloe gel on germs can further enhance the conditioning effect of the dipotassium glycyrrhizinate on the skin, and meanwhile, the good water retention property of the dipotassium glycyrrhizinate, the aloe gel and the marine hydrolyzed collagen are beneficial to the absorption of the potassium glycyrrhizinate and the marine hydrolyzed collagen by the skin. The marine hydrolyzed collagen can inhibit the deposition of pigment, provide amino acids for skin metabolism, improve the living environment of skin cells, enhance the activity and resistance of skin, and assist dipotassium glycyrrhizinate and aloe gel to play the role of conditioning and treating.

In some specific embodiments, the marine hydrolyzed collagen is 1 part, 5 parts, or 10 parts by weight.

In some specific embodiments, the dipotassium glycyrrhizinate is 0.5 parts, 2 parts, or 4 parts.

In some specific embodiments, the aloe gel powder is 0.2 parts, 0.5 parts, or 1 part.

In some specific embodiments, the marine hydrolyzed collagen has a molecular weight of 400 to 1500 daltons, e.g., 400 daltons, 500 daltons, 600 daltons, 700 daltons, 800 daltons, 900 daltons, 1000 daltons, 1100 daltons, 1200 daltons, 1300 daltons, or 1400 daltons.

In some embodiments, the marine hydrolyzed collagen has a Polymer Dispersion Index (PDI) of less than or equal to 3.0, preferably a PDI of less than or equal to 2.4, and more preferably a PDI of less than or equal to 2.0.

In some specific embodiments, the marine hydrolyzed collagen is selected from Nippi hydrolyzed collagen, rosilol hydrolyzed collagen, eastern marine hydrolyzed collagen, or Shandongtian hydrolyzed collagen.

The present invention also unexpectedly finds that the molecular weight size and PDI value affect the inhibition of tyrosinase by marine hydrolyzed collagen. Therefore, the method of the invention also limits the molecular weight and PDI value of the marine hydrolyzed protein, and the marine hydrolyzed protein in the range has good tyrosinase inhibition effect.

In some embodiments, the pH of the dressing solution of the present invention is 4.0 to 6.0, preferably 5.0 to 6.0, for use on human skin.

In some embodiments, the aloe gel powder is aloe vera gel powder.

The invention also relates to a dressing for treating acne, said dressing comprising a dressing substrate and the aforementioned dressing liquid.

In some specific embodiments, the dressing substrate is a nonwoven fabric, preferably, the nonwoven fabric is a chitosan nonwoven fabric; more preferably, the mass fraction of chitosan in the nonwoven fabric is not less than 20%, or not less than 30%, or not less than 50%, or not more than 100%.

The base of the dressing is the non-woven fabric containing chitosan, wherein the chitosan has excellent antibacterial activity, has good antibacterial effect on staphylococcus aureus, escherichia coli, lactobacillus and other common strains, and can further enhance the treatment effect of the dressing on acne.

In some embodiments, the dressing substrate is impregnated with the dressing solution, or the dressing substrate and the dressing solution are packaged separately.

Compared with the prior art, the invention has the beneficial effects that:

(1) the dressing liquid and the dressing have the effects of killing main pathogenic bacteria of acne, conditioning skin and inhibiting pigmentation by matching dipotassium glycyrrhizinate, aloe gel active substances, marine hydrolyzed collagen polypeptide and/or chitosan, and have good treatment and repair effects on the acne on the whole.

(2) The dressing liquid and the dressing mainly adopt pure natural materials, and have the advantages of simplicity, safety, good histocompatibility and no stimulation to skin.

(3) The invention further defines the molecular weight and PDI value of the marine hydrolyzed collagen, and can further enhance the inhibition effect of the dressing liquid and the dressing on the tyrosinase activity.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a diagram of the skin condition of the right face of a subject after treatment is complete;

fig. 2 is the skin condition of the left face of the subject after treatment is complete.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by manufacturers, and are all conventional products available on the market.

Example 1

A dressing for treating acne comprises a dressing liquid and a dressing substrate, wherein the dressing liquid is mainly prepared from 1 part of marine hydrolyzed collagen, 0.5 part of dipotassium glycyrrhizinate, 0.2 part of aloe gel powder and 1000 parts of water in parts by weight; the dressing substrate is a chitosan non-woven fabric, wherein the mass fraction of chitosan is 20%.

The marine hydrolyzed collagen is Nippi hydrolyzed collagen, and the aloe gel powder is aloe vera gel powder.

The specific preparation method of the auxiliary materials comprises the following steps: 1. adding marine hydrolyzed collagen, dipotassium glycyrrhizinate and aloe gel powder into purified water under sterile environment, stirring and dissolving to obtain auxiliary material liquid. 2. Mixing the auxiliary material liquid with chitosan non-woven fabric in an aseptic environment, packaging, and then performing irradiation sterilization.

Example 2

Dressings were prepared as described with reference to example 1, except that: the dressing liquid is mainly prepared from 5 parts of marine hydrolyzed collagen, 2 parts of dipotassium glycyrrhizinate, 1 part of aloe gel powder and 1000 parts of water; the mass fraction of chitosan in the non-woven fabric is 30%.

Example 3

Dressings were prepared as described with reference to example 1, except that: the dressing liquid is mainly prepared from 10 parts of marine hydrolyzed collagen, 4 parts of dipotassium glycyrrhizinate, 1 part of aloe gel powder and 1000 parts of water; the mass fraction of chitosan in the non-woven fabric is 100%.

Example 4

Dressings were prepared as described with reference to example 2, except that: the mass fraction of chitosan in the non-woven fabric is 50%.

Example 5

Dressings were prepared as described with reference to example 2, except that: the mass fraction of chitosan in the non-woven fabric is 100%.

Example 6

A dressing was prepared as described with reference to example 1, except that the marine hydrolyzed collagen was rosylor marine hydrolyzed collagen.

Example 7

The dressing was prepared according to the method described in example 1, except that the marine hydrolyzed collagen was the oriental marine hydrolyzed collagen.

Example 8

The dressing was prepared as described with reference to example 1, except that the marine hydrolyzed collagen was Shandong permanent marine hydrolyzed collagen.

Example 9

A dressing is prepared as described with reference to example 1, except that the dressing liquid is packaged and sterilized separately from the dressing substrate during the preparation process.

Experimental example 1

The physical and chemical properties of the marine hydrolyzed collagen and the tyrosinase inhibition rate thereof used in the previous examples were measured, wherein specific measurement modes and measurement results are shown below.

1. The molecular weight and the Polymer Dispersibility Index (PDI) of different brands of marine hydrolyzed collagen (Nippi, Rocelo, eastern sea and Shandongtian time) were measured by high performance size exclusion chromatography, and the specific measurement results are shown in Table 1.

TABLE 1 physicochemical Properties of different marine hydrolyzed collagens

2. The mushroom tyrosinase dopa rate oxidation method is adopted to detect the tyrosinase inhibition rates of different brands of marine hydrolyzed collagen, and the specific detection method and detection results are shown as follows.

1) Sample treatment: preparing 10g/L solution (tyrosinase is copper protein, and the solution should not contain copper ions) of marine hydrolyzed collagen of different brands with ultrapure water, and using the solution as sample liquid for later use.

2) And (3) experimental operation: performing biochemical reaction in a glass tube, adding required phosphate buffer solution with pH6.8 (6.8 g of dipotassium phosphate and 0.944g of sodium hydroxide with constant volume of 1000ml), sample liquid, kojic acid solution (10g/L) and enzyme solution (about 3.5mg of tyrosinase with constant volume of 100ml) into each tube, reacting for 10min in water bath at 37 ℃, sequentially adding L-dopa solution (2.5g/L), reacting for 10min in water bath at 37 ℃, and measuring the light absorption value at the wavelength of 475 nm. The experimental system design is shown in table 2.

Table 2 design of experimental system

3) The inhibition rate calculation method comprises the following steps: the intensity of inhibition of tyrosinase activity by the sample is expressed as the inhibition rate. The inhibition rate of the sample is [ positive control absorbance value- (sample tube absorbance value-negative control absorbance value) ]/positive control absorbance value multiplied by 100%, the inhibition rate of the standard control is [ positive control absorbance value-standard tube absorbance value)/positive control absorbance value multiplied by 100%, and the high inhibition rate indicates that the inhibition rate of the standard control absorbance value on the enzyme activity is the highest. Reference of standard substance: in order to ensure the accuracy of the experimental result, a standard substance control is set in the laboratory for detection. Kojic acid is selected as a standard control, the inhibition rate of the kojic acid standard control is different in different batches of experiments, the inhibition rate of the standard control can be converted into 100%, and the inhibition rates of the tested substances are compared after being changed in equal proportion. The inhibition ratio of the test substance is calculated to be 18 percent, the inhibition ratio of the standard control substance is calculated to be 90 percent, and the inhibition ratio of the test substance after conversion is 18 percent/90 percent to 20 percent according to the formula.

TABLE 3 tyrosinase inhibition rates for marine hydrolyzed collagen of different brands

2. And (3) analyzing a detection result: as can be seen from the molecular weight and polymer dispersibility index of marine hydrolyzed collagen shown in table 1 and the tyrosinase inhibition rate shown in table 3, the tyrosinase inhibition rate of marine hydrolyzed collagen is related to its molecular weight, PDI, etc., and marine hydrolyzed collagen having a molecular weight of 1000 daltons or less and a PDI index of 2.4 or less has a good inhibitory effect on tyrosinase.

Experimental example 2

The cytotoxicity of the raw materials and the finished dressing used in example 5 is detected by referring to the national standard GB/T16886.5-2003/ISO 10993-5:1999, and the specific detection results are shown in Table 4.

And (3) carrying out toxicity classification and qualification judgment on the sample according to the relative cell proliferation rate (RGR) obtained by detection, wherein the specific classification standard is as follows:

level 0: RCG is more than or equal to 100 percent; level 1: RCG is more than or equal to 75% at 99%; and 2, stage: RCG is more than or equal to 74 and more than or equal to 50; and 3, level: RCG is more than or equal to 49 and more than or equal to 25; 4, level: RGR is more than or equal to 24 and more than or equal to 1; grade 5 is 0.

And (3) sample qualification judgment standard: the grade 0-1 is qualified; and judging whether the grade 2 is qualified or not and whether the grade 3-5 is unqualified or not according to the comprehensive analysis of the cell morphology.

According to the experimental results shown in Table 4, the raw materials and the finished products related to the invention have no cytotoxicity, and are safe and reliable.

TABLE 4 results of cytotoxicity test

Experimental example 3

The dressings in examples 1-5 are taken to detect the inhibition effect of the dressings on staphylococcus aureus (ATCC6538) and propionibacterium acnes (ATCC11827), the specific detection method is shown in national standard GB/T20944, and the specific detection result is shown in Table 5. According to the test results shown in table 5, the dressing provided by the invention has good bacteriostatic action on staphylococcus aureus and propionibacterium acnes.

TABLE 5 results of the bacteriostatic test

Experimental example 4

Two subjects were recruited and tested on the dressing described in example 5, as follows:

1. test subject

Quantity: 2, name of the Chinese medicinal composition; sex: male; age: 25-33 years old; physiological conditions: moderate acne on the face, red and swollen all the year round, and hard acne.

2. Test method

Half face experiment, 2 persons used the dressing of the invention described in example 4 for the right half face, and the left half face as a control. The experimental period is 4 weeks, the dressing is used for 1 time every day in the first 10 days, the use time is 20-30 min/time, and the dressing is used for 2-3 times every week later, and the use time is 20-30 min/time.

3. Test results

After the test is finished, 7-8 people are called to perform blind evaluation on the left and right face skin conditions of two subjects, the facial acne of the right side skin of the two subjects is obviously reduced, scars are reduced, the glossiness is increased, and the overall skin condition is obviously superior to that of the left side skin. The control results of the left and right facial skins of one subject after the test are shown in fig. 1-2, and it can be seen from the results shown in the figures that the acne condition of the right face of the subject is obviously improved after the treatment by the dressing.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A dressing for treating acne, characterized in that the dressing consists of a dressing substrate and a dressing liquid;

the dressing liquid is prepared from 1-10 parts by weight of marine hydrolyzed collagen, 0.5-4 parts by weight of dipotassium glycyrrhizinate, 0.2-1 part by weight of aloe gel powder and 1000 parts by weight of water;

the pH value of the dressing liquid is 4.0-6.0;

the molecular weight of the marine hydrolyzed collagen is 400-1500 daltons;

the Polymer Dispersion Index (PDI) of the marine hydrolyzed collagen is less than or equal to 3.0;

the marine hydrolyzed collagen is selected from Nippi hydrolyzed collagen, Luosiro hydrolyzed collagen, eastern marine hydrolyzed collagen or Shandongtian hydrolyzed collagen;

the aloe gel powder is aloe vera gel powder;

the dressing substrate is non-woven fabric;

the non-woven fabric is chitosan non-woven fabric;

the mass fraction of chitosan in the non-woven fabric is more than or equal to 20 percent;

the dressing substrate and the dressing liquid are packaged separately.

2. The dressing of claim 1, wherein the marine hydrolyzed collagen is 1, 5, or 10 parts by weight; the dipotassium glycyrrhizinate accounts for 0.5 part, 2 parts or 4 parts; the aloe gel powder is 0.2 part, 0.5 part or 1 part.

3. The dressing of claim 1, wherein the marine hydrolyzed collagen has a molecular weight of 400 daltons, 500 daltons, 600 daltons, 700 daltons, 800 daltons, 900 daltons, 1000 daltons, 1100 daltons, 1200 daltons, 1300 daltons, or 1400 daltons.

4. The dressing of claim 1, wherein the marine hydrolyzed collagen has a PDI of 2.4 or less.

5. The dressing of claim 1, wherein the marine hydrolyzed collagen has a PDI of 2.0 or less.

6. The dressing of claim 1, wherein the pH of the dressing solution is 5.0-6.0.