CN108191971B - 重组人金属硫蛋白Ⅲα片段纯品的制备方法 - Google Patents
重组人金属硫蛋白Ⅲα片段纯品的制备方法 Download PDFInfo
- Publication number
- CN108191971B CN108191971B CN201810000449.6A CN201810000449A CN108191971B CN 108191971 B CN108191971 B CN 108191971B CN 201810000449 A CN201810000449 A CN 201810000449A CN 108191971 B CN108191971 B CN 108191971B
- Authority
- CN
- China
- Prior art keywords
- buffer solution
- recombinant human
- solution
- preparing
- supernatant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/825—Metallothioneins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810000449.6A CN108191971B (zh) | 2018-01-02 | 2018-01-02 | 重组人金属硫蛋白Ⅲα片段纯品的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810000449.6A CN108191971B (zh) | 2018-01-02 | 2018-01-02 | 重组人金属硫蛋白Ⅲα片段纯品的制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108191971A CN108191971A (zh) | 2018-06-22 |
CN108191971B true CN108191971B (zh) | 2022-02-22 |
Family
ID=62587967
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810000449.6A Active CN108191971B (zh) | 2018-01-02 | 2018-01-02 | 重组人金属硫蛋白Ⅲα片段纯品的制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108191971B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110734490A (zh) * | 2019-11-28 | 2020-01-31 | 苏州华研医用科技有限公司 | 重组人金属硫蛋白ⅲ纯品的制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101033254A (zh) * | 2007-02-09 | 2007-09-12 | 广州市恺泰生物科技有限公司 | 一种纯化重组人白细胞介素12的方法 |
CN102660574A (zh) * | 2012-02-10 | 2012-09-12 | 汪志友 | 一种建立双启动子甲醇酵母高效表达重组人金属硫蛋白系统的方法及该蛋白的制备纯化 |
CN105017412A (zh) * | 2015-07-24 | 2015-11-04 | 中华人民共和国广州机场出入境检验检疫局 | 一种从牛血清中分离高纯度牛血清白蛋白的方法 |
CN106279437A (zh) * | 2016-08-19 | 2017-01-04 | 安源医药科技(上海)有限公司 | 高糖基化人凝血因子viii融合蛋白及其制备方法与用途 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI592419B (zh) * | 2014-11-11 | 2017-07-21 | 承帝 司馬 | 製造具有氧化還原活性之低金屬化金屬硫蛋白的方法及含彼之醫藥組合物 |
-
2018
- 2018-01-02 CN CN201810000449.6A patent/CN108191971B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101033254A (zh) * | 2007-02-09 | 2007-09-12 | 广州市恺泰生物科技有限公司 | 一种纯化重组人白细胞介素12的方法 |
CN102660574A (zh) * | 2012-02-10 | 2012-09-12 | 汪志友 | 一种建立双启动子甲醇酵母高效表达重组人金属硫蛋白系统的方法及该蛋白的制备纯化 |
CN105017412A (zh) * | 2015-07-24 | 2015-11-04 | 中华人民共和国广州机场出入境检验检疫局 | 一种从牛血清中分离高纯度牛血清白蛋白的方法 |
CN106279437A (zh) * | 2016-08-19 | 2017-01-04 | 安源医药科技(上海)有限公司 | 高糖基化人凝血因子viii融合蛋白及其制备方法与用途 |
Non-Patent Citations (3)
Title |
---|
"人金属硫蛋白-3的α结构域的表达及其性质研究";王寒松等;《北京大学学报(自然科学版)》;20010720;第37卷(第4期);第471页最后一段至472页第一段和第474页讨论 * |
"金属硫蛋白(MT)的分离纯化与检测技术";王达等;《江西科学》;20040215;第22卷(第1期);第62页右栏第一段 * |
"集胞藻类金属硫蛋白的纯化、性质和溶液构象的研究";陈正佳等;《植物学报》;19990225;第41卷(第2期);第151页1.2集泡藻MT-like的分离纯化 * |
Also Published As
Publication number | Publication date |
---|---|
CN108191971A (zh) | 2018-06-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR100392021B1 (ko) | 재조합 사람혈청알부민의 정제방법 | |
CN107033238B (zh) | 一种重组人源iii型胶原蛋白的纯化方法和制备方法 | |
Caprioli et al. | A cell division-active protein from E. coli | |
US10981975B2 (en) | Method for efficient purification of human serum albumin | |
CN113150120B (zh) | 发酵液中猪肌红蛋白的分离纯化方法 | |
CN108070032A (zh) | 一种重组人源胶原蛋白的纯化方法 | |
MXPA02006303A (es) | Proceso para la purificacion de proteinas farmacologicamente activas a traves de cromografia del intercambio cationico. | |
HUE027724T2 (hu) | Eljárás G-CSF tisztítására | |
CN112210002A (zh) | 重组人血清白蛋白的纯化方法 | |
JP2015508664A (ja) | カラムでの酵素的切断 | |
CN104726478A (zh) | 表达精氨酸脱亚胺酶基因的重组大肠杆菌及其应用 | |
CN108191971B (zh) | 重组人金属硫蛋白Ⅲα片段纯品的制备方法 | |
Khan et al. | Large-scale production of recombinant proteins: human leukocyte interferon | |
Kučera | Fungal mycelium—the source of chitosan for chromatography | |
EP0446582B1 (en) | Method for producing of recombinant human cysteineless gamma-interferon free of methionine at n-terminal | |
CN109321539B (zh) | 一种l-谷氨酸氧化酶纯化方法 | |
CN106222221A (zh) | 制备重组人粒细胞刺激因子原液的纯化方法 | |
US11639515B2 (en) | Genetically engineered strain for producing porcine myoglobin and food-grade fermentation and purification thereof | |
WO1992002616A1 (en) | Purification of erwinia-l-asparaginase | |
RU2230325C2 (ru) | Способ приготовления очищенного препарата ботулинического токсина типа а | |
JP7454507B2 (ja) | ボツリヌス毒素の製造方法 | |
CN110904113B (zh) | 无标签人半乳糖凝集素10晶体及其制备方法和应用 | |
CN1141395C (zh) | 金属硫蛋白的制备方法 | |
KR100356738B1 (ko) | 염기성 단백질로부터 엔도톡신을 제거하는 방법 | |
CN110734490A (zh) | 重组人金属硫蛋白ⅲ纯品的制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20191204 Address after: 215500 Daming Road, High-tech Industrial Park, Changshu Economic Development Zone, Suzhou City, Jiangsu Province Applicant after: Suzhou Huayan Medical Technology Co., Ltd Address before: 215500 69 buildings in Meiyuan villa, Yushan forest farm, Suzhou, Jiangsu, Changshou City Applicant before: Feng Yongliang |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20210804 Address after: 201107 room a0216, floor 1, building 104, No. 1-30, Lane 88, Minbei Road, Minhang District, Shanghai Applicant after: Brinker Technology (Shanghai) Co.,Ltd. Address before: 215500 No. 8 Daming Road, high tech Industrial Park, Changshu Economic Development Zone, Suzhou, Jiangsu Applicant before: Suzhou Huayan Medical Technology Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20220121 Address after: 215500 Daming Road, High-tech Industrial Park, Changshu Economic and Technological Development Zone, Suzhou City, Jiangsu Province Applicant after: Huihai (Suzhou) Biotechnology Co.,Ltd. Address before: 201107 room a0216, floor 1, building 104, No. 1-30, Lane 88, Minbei Road, Minhang District, Shanghai Applicant before: Brinker Technology (Shanghai) Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant |