CN108186438B - After-sun repair gel containing cell growth factors and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of cosmetics, and relates to a postsolarization repairing gel containing cell growth factors and a preparation method thereof. The invention relates to a post-basking repair gel containing cell growth factors, which comprises the following components: cell growth factor complex liposome, rose hip oil, phytic acid, mint extract, eucheuma extract, carbomer 940, polyglutamic acid, allantoin, propylene glycol, PEG-40 hydrogenated castor oil, tocopherol acetate, bisabolol, preservative, essence and deionized water. The suncured repair gel containing the cell growth factors has a reasonable formula and stable properties, can effectively keep the activity of the cell growth factors and improve the bioavailability of the cell growth factors through the synergistic effect of all components, has obvious water replenishing and moisturizing effects, has obvious effects of relieving and accelerating the recovery of the suncured skin caused by sunburn and other symptoms such as diffuse erythema, redness, desquamation, blisters and the like, and can relieve pigmentation and blacken the skin.

Description

After-sun repair gel containing cell growth factors and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and relates to a postsolarization repairing gel containing cell growth factors and a preparation method thereof.

Background

The long-wave ultraviolet UVA can penetrate the epidermis and most of the dermis of the skin, oxidize reduced melanin in the epidermis to directly tan the skin, and damage elastic fibers in the skin after long-term action, so that the skin is photo-aged and seriously damaged. The medium-wave ultraviolet UVB has weaker action capability on skin than UVA, most of the medium-wave ultraviolet UVB is absorbed by the epidermis, and a small amount of the medium-wave ultraviolet UVB penetrates through the epidermis to denature nucleic acid or protein in epidermal cells of the skin and generate acute dermatitis erythema or burning.

Sunburn is caused by overexposure of the skin to ultraviolet rays, symptoms and signs of which generally appear within 1-24 hours after sunburn, and skin changes from mild erythema with transient scale formation to pain, edema, skin tenderness and bulla. The first-degree sunburn is characterized in that diffuse erythema appears on local skin after sunburn, the boundary is clear, and the peak is reached in 24-36 hours. Second degree sunburn is characterized by redness and swelling of local skin, followed by blister or bullous blister, tense blister wall, yellowish blister fluid with burning pain or itching, erosion after blister rupture, dry scab, and residual pigmentation or hypopigmentation.

A series of biological injuries of the skin caused by ultraviolet rays seriously affect the skin health of people, and the skin is protected in time after being dried by the sun, thereby being beneficial to relieving sunburn symptoms and accelerating the skin recovery. The existing after-sun repair products mainly supplement water and preserve moisture, and the effect of promoting after-sun skin repair is not obvious. Chinese patent application CN 102846514A discloses an aqueous gel containing tea polyphenol for after-sun repair, which comprises beta-glucan, tea polyphenol, alkanna tinctoria root extract, witch hazel extract, trehalose, a thickening agent, a humectant, a preservative, a chelating agent, a nutritional agent, a neutralizing agent, essence, deionized water and the like, has good water replenishing and moisturizing effects, can relieve red swelling and pain symptoms of skin after sun burn, can be used for after-sun repair of first-level sun burn, has a certain effect of promoting skin repair, but has an unobvious after-sun repair effect on second-level sun burn, is slow in skin recovery, has no protective effect on skin after sun burn after use, and is more vulnerable to ultraviolet rays after scales and blisters are crusted and removed.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a post-basking repair gel containing cell growth factors. The suncured repair gel containing the cell growth factor has obvious effects of relieving and accelerating the recovery of the suncured skin caused by ultraviolet radiation, such as diffuse erythema, redness and swelling, blisters and the like.

The technical scheme of the invention is as follows:

the postsolarization repair gel containing the cell growth factors comprises the following components in percentage by weight: 1-2% of cell growth factor composite liposome, 2-4% of rose hip oil, 1-4% of phytic acid, 2-4% of mint extract, 3-6% of eucheuma extract, 9401-2.5% of carbomer, 0.05-0.2% of polyglutamic acid, 0.1-0.3% of allantoin, 1-5% of propylene glycol, 0.05-0.2% of PEG-40 hydrogenated castor oil, 0.3-0.6% of tocopherol acetate, 0.1-0.4% of bisabolol, 0.05-1% of preservative, 0.01-0.05% of essence and 72-88% of deionized water;

in the cell growth factor compound liposome, the cell growth factor is selected from one or more of recombinant human epidermal growth factor, recombinant human acidic fibroblast growth factor, recombinant human basic fibroblast growth factor, recombinant human keratinocyte growth factor, recombinant human vascular endothelial growth factor, recombinant human insulin-like growth factor and recombinant human platelet-derived growth factor.

In some embodiments, the post-basking repair gel containing the cell growth factor comprises the following components in percentage by weight: 1.2% of cell growth factor complex liposome, 2% of rose hip oil, 1.5% of phytic acid, 3% of mint extract, 5% of eucheuma extract, 9401.6% of carbomer, 0.1% of polyglutamic acid, 0.15% of allantoin, 3% of propylene glycol, 0.1% of PEG-40 hydrogenated castor oil, 0.4% of tocopherol acetate, 0.2% of bisabolol, 0.1% of preservative, 0.01% of essence and the balance of deionized water; in the cell growth factor compound liposome, the cell growth factors are recombinant human basic fibroblast growth factors and recombinant human vascular endothelial cell growth factors.

The after-sun repair gel contains cell growth factors, and the cell growth factors can act with skin cells, promote the nutrition metabolism of epithelial cells, protect the skin, simultaneously promote the functions of subcutaneous collagen cells, quickly repair damaged cells, accelerate the after-sun skin recovery and prevent the next damage of ultraviolet rays to the skin.

The mint extract is an extract of aerial parts of mint Menthahal palyoxBriq, a mint plant of the genus Mentha of the family Labiatae; the Eucheuma Gelatinosum extract is extract of Eucheuma Gelatinosum Eucheuma muricatum (Gmel.) of Solieriaceae and Eucheuma Gelatinosum Eucheuma okamurai Yamada. The rose hip oil (CAS:84603-93-0), the mint extract and the Eucheuma Gelatinosum extract have the functions of deeply moistening skin, supplementing water, moisturizing and enhancing skin tissue activity, can quickly relieve the symptoms of erythema, redness and swelling after sunburn of the skin, and can prevent the skin from being blackened due to skin pigmentation.

The phytic acid, carbomer 940, polyglutamic acid, allantoin, propylene glycol, PEG-40 hydrogenated castor oil, tocopherol acetate, bisabolol and the like are taken as matrix auxiliary materials, and the prepared after-sun repair gel containing the cell growth factors is stable in system, beneficial to improving the effects of moisturizing the skin, and simultaneously beneficial to preventing the oxidation of the cell growth factor composite liposome and maintaining the activity of the cell growth factors.

In some embodiments, the preservative of the suncured gel containing cell growth factors of the present invention may be one or more of conventional preservatives available in the market, such as paraben preservatives (e.g., methylparaben and ethylparaben), 1, 2-diol preservatives (e.g., pentanediol, hexanediol, octanediol and ethylhexylglycerin), p-hydroxyacetophenone, and the like, and may also be other preservatives having antibacterial and antiseptic effects that can be used in cosmetics. The preservative of the invention is preferably housefly antibacterial peptide. The housefly antibacterial peptide is an antibacterial active substance obtained by inducing housefly larvae to secrete by a method of injury infection, and has broad-spectrum antibacterial activity. The housefly antibacterial peptide has obvious antibacterial activity, small irritation to skin and strong stability, and is beneficial to prolonging the shelf life of products; the housefly antibacterial peptide is a peptide substance and is beneficial to keeping the activity of cell growth factors; in addition, the housefly antibacterial peptide is also beneficial to promoting the repair of skin injury after being sunned and promoting the scab and desquamation of the blisters.

In some embodiments, the cell growth factor complex liposome of the after-sun repair gel containing the cell growth factor comprises the following components in parts by weight: 0.002-0.006 part of recombinant human basic fibroblast growth factor, 0.001-0.002 part of recombinant human vascular endothelial cell growth factor, 2-10 parts of deep sea fish skin collagen zymolyte, 4-8 parts of peanut sprout extract, 80-120 parts of hydrogenated lecithin, 1-3 parts of sodium cholate and 20-30 parts of cholesterol.

The recombinant human basic fibroblast growth factor and the recombinant human vascular endothelial growth factor are used together, so that the cells can be promoted to generate nutrients such as hyaluronic acid, collagen and the like, and meanwhile, the skin can be promoted to absorb external nutrients, damaged cells can be quickly repaired, and the damage of ultraviolet rays to the skin can be reduced. On the other hand, the combined use of the recombinant human basic fibroblast growth factor and the recombinant human vascular endothelial cell growth factor can also promote cell division and proliferation, effectively eliminate aging and metabolism disorder cells, reduce the content of melanin and colored cells in skin cells and lighten skin pigmentation.

The deep sea fish skin collagen zymolyte and the peanut sprout extract contain rich protein, micromolecular polypeptide, resveratrol and other components, have obvious moisture retention and antioxidation effects, have good protection effect on the recombinant human basic fibroblast growth factor and the recombinant human vascular endothelial growth factor, can well maintain the biological activity of the recombinant human basic fibroblast growth factor and the recombinant human vascular endothelial growth factor, and in addition, the permeability of each active component of the compound liposome can be improved by the synergy of the four components, thereby being beneficial to the absorption of the skin on each active component and improving the bioavailability of the compound liposome.

In some embodiments, the method for preparing the deep sea fish skin collagen hydrolysate comprises the following steps: adding water into deep sea fish skin collagen powder according to a solid-to-liquid ratio of 20-40 g/L, adding 0.01-0.05% of hydrolyzed cod skin protein by weight of the deep sea fish skin collagen powder, stirring for 15-30 min, adding 5-10% of compound protease by weight of the solution, carrying out enzymolysis for 5-8 h at the temperature of 45-55 ℃ and under the condition that the pH value is 6.0-7.0, carrying out enzyme deactivation at 100 ℃ for 5-10 min after the enzymolysis is finished, filtering, concentrating the filtrate under reduced pressure, and drying to obtain a deep sea fish skin collagen hydrolysate; the composite protease is composed of collagenase and bromelain according to a weight ratio of (4-10) to 1.

The hydrolyzed cod skin protein has dispersing, moistening and penetrating abilities, and is added in the deep sea fish skin collagen enzymolysis process to promote the enzymolysis of the deep sea fish skin collagen. The combined use of the collagenase and the bromelain is beneficial to promoting the further breaking of the collagen peptide chain of the deep-sea fish skin, improving the enzymolysis rate of the deep-sea fish skin collagen and obtaining the small molecular polypeptide.

In some embodiments, the method of preparing a peanut sprout extract comprises the steps of: taking peanuts, sterilizing, soaking seeds and accelerating germination, selecting peanut sprouts without fibrous roots and with root lengths of 0.2-1.5 cm, removing seed coats, homogenizing, adding water with the weight of 10-20 times of that of the peanut sprouts, carrying out ultrasonic extraction for 1-2 times at the temperature of 40-60 ℃, carrying out 30-60 min each time, filtering, combining filtrates, concentrating the filtrate under reduced pressure to 30-40% of the original volume, adding ethanol until the alcohol content is 70-80%, standing for 8-12 h at the temperature of 4-10 ℃, filtering, concentrating the filtrate under reduced pressure, and drying to obtain the peanut sprout extract. When peanut sprouts are prepared, peanuts with full seeds produced in the same year are preferably selected, the peanuts can be disinfected by 70-75% of ethanol, 5-10% of sodium hypochlorite or 5-10% of hydrogen peroxide, then seeds are soaked in warm water at 18-23 ℃ for 12-20 hours, and then germination is performed in a dark environment, wherein the preferable temperature condition is 20-25 ℃.

In some embodiments, the method for preparing the cell growth factor complex liposome comprises the following steps:

1) adding hydrogenated lecithin, sodium cholate and cholesterol into absolute ethyl alcohol according to the solid-to-liquid ratio of 2-5 mg/mL, and stirring for dissolving to obtain a solution I;

2) adding PBS into the recombinant human basic fibroblast growth factor, the recombinant human vascular endothelial cell growth factor, the deep-sea fish skin collagen hydrolysate and the peanut sprout extract according to the solid-to-liquid ratio of 4-8 mg/mL, and stirring and dissolving to obtain a solution II;

3) adding the solution I obtained in the step 1) and the solution II obtained in the step 2) into a supercritical autoclave, wherein the temperature is 30-40 ℃, the pressure is 20-30 MPa, and CO is added₂Emulsifying for 30-45 min under the condition of the flow rate of 12-16L/h, and separating an emulsified product under reduced pressure to obtain a cytokine complex liposome; wherein the separation temperature is 30-40 ℃, and the separation pressure is 5-15 MPa.

PBS (Phosphate Buffered Saline) is composed mainly of Na₂HPO₄、KH₂PO₄The buffer solution prepared from NaCl and KCl has wide application range of buffered pH value, in some embodiments, the pH value of PBS is 7.2-7.6, and the activity of the recombinant human basic fibroblast growth factor and the recombinant human vascular endothelial growth factor is higher in the pH range.

Correspondingly, the invention also provides a preparation method of the postsolarization restoration gel containing the cell growth factors, which comprises the following steps:

s1: adding deionized water into carbomer 940 and polyglutamic acid, dispersing and swelling, adding phytic acid, allantoin, rose hip oil, PEG-40 hydrogenated castor oil, propylene glycol, tocopheryl acetate and bisabolol into an emulsifying pot, heating to 80-85 ℃, stirring at constant temperature until the materials are completely dissolved to obtain a material A, preserving the temperature of the material A for 10-15 min, defoaming and cooling;

s2, dissolving the mint extract and the eucheuma extract in water to obtain a mixed solution I;

s3, when the temperature of the material A in the step S1 is reduced to 40-45 ℃, adding the cell growth factor composite liposome, the mixed liquid I in the step S2, the preservative and the essence, stirring and uniformly mixing, sampling and detecting, and discharging qualified products to obtain the compound liposome.

The suncured repair gel containing the cell growth factor has the advantages of reasonable formula, stable property and mild effect, can effectively maintain the activity of the cell growth factor and improve the bioavailability of the cell growth factor through the synergistic effect of all the components, has obvious water supplementing and moisturizing effects, has obvious effects of relieving and accelerating the recovery of the suncured skin caused by ultraviolet radiation, such as diffuse erythema, redness, blisters and the like of the skin, and can simultaneously avoid the skin from being blackened due to pigmentation and prevent the next damage of the ultraviolet to the skin.

Detailed Description

The present invention will be further described below by way of specific embodiments, but the present invention is not limited to only the following examples. In the examples of the present invention, unless otherwise specified, all the raw materials used are conventional commercially available products, such as deep sea fish skin collagen powder purchased from Guangzhou Strong Producer science and technology Limited; hydrolyzed cod skin protein was purchased from shanghai habao chemical limited; mint extract and Eucheuma extract were purchased from American-exemplary Biotech, Inc., Guangzhou.

The invention relates to a preparation method of housefly antibacterial peptide, which comprises the following steps: zhouyu, Yi Bing, light-coated, etc. preliminary study on the antibacterial activity and antibacterial mechanism of housefly antibacterial peptide [ J ] Chinese antibiotic impurities, 2004, 5 (29).

Example 1 preparation of collagen hydrolysate of deep sea fish skin

The method comprises the following steps:

adding water into deep sea fish skin collagen powder according to a solid-to-liquid ratio of 30g/L, adding hydrolyzed cod fish skin protein which accounts for 0.02% of the weight of the deep sea fish skin collagen powder, stirring for 30min, adding compound protease which accounts for 8% of the total weight of the solution, carrying out enzymolysis for 8h at the temperature of 50 ℃ and the pH value of 6.2, inactivating enzyme at the temperature of 100 ℃ for 6min after the enzymolysis is finished, filtering, concentrating the filtrate under reduced pressure, and drying to obtain a deep sea fish skin collagen hydrolysate; wherein the compound protease is composed of collagenase and bromelain according to the weight ratio of 8: 1.

Example 2 preparation of peanut sprout extract

The method comprises the following steps:

selecting peanuts full in the current year, disinfecting the peanuts by using 10% hydrogen peroxide, soaking the peanuts in warm water at 20 ℃ for 12 hours, then accelerating germination in a dark environment, carrying out ultrasonic extraction for 2 times at 50 ℃ for 40min each time, filtering, combining filtrates, concentrating the filtrate under reduced pressure to 30% of the original volume, adding ethanol to 80%, standing at 4 ℃ for 10 hours, filtering, concentrating the filtrate under reduced pressure, and drying to obtain the peanut sprout extract, wherein the temperature condition is 20-25 ℃, the peanut sprouts without fibrous roots and with root length of 0.2-1.5 cm are selected, the seed coats are removed and homogenized, water with the weight being 20 times of that of the peanut sprouts is added, the ultrasonic extraction is carried out for 2 times at 50 ℃, the filtrate is filtered, the filtrate is combined, and the filtrate is dried to obtain the peanut sprout extract.

EXAMPLE 3 preparation of cell growth factor Complex liposomes

The cell growth factor composite liposome comprises the following components in parts by weight: 0.005 part of recombinant human basic fibroblast growth factor, 0.001 part of recombinant human vascular endothelial growth factor, 7 parts of deep-sea fish skin collagen zymolyte prepared in example 1, 5 parts of peanut sprout extract prepared in example 2, 100 parts of hydrogenated lecithin, 2 parts of sodium cholate and 25 parts of cholesterol.

The preparation method comprises the following steps:

1) adding hydrogenated lecithin, sodium cholate and cholesterol into absolute ethyl alcohol according to the solid-to-liquid ratio of 3mg/mL, and stirring and dissolving to obtain a solution I;

2) adding PBS (phosphate buffer solution) with the pH of 7.4 into the recombinant human basic fibroblast growth factor, the recombinant human vascular endothelial cell growth factor, the deep-sea fish skin collagen hydrolysate and the peanut sprout extract according to the solid-to-liquid ratio of 5mg/mL, and stirring and dissolving to obtain a solution II;

3) adding the solution I obtained in the step 1) and the solution II obtained in the step 2) into a supercritical autoclave, wherein the temperature is 35 ℃, the pressure is 25MPa, and CO is added₂Emulsifying for 45min under the condition of the flow rate of 15L/h, and separating an emulsified product under reduced pressure to obtain a cytokine compound liposome; wherein the separation temperature is 30 ℃ and the separation pressure is 10 MPa.

Examples 4-6 preparation of post-basking repair gels containing cell growth factor

Examples 4-6. the after-sun repair gel containing the cell growth factor comprises the following components in percentage by weight:

the preparation method comprises the following steps:

s1, adding deionized water into carbomer 940 and polyglutamic acid, dispersing and swelling with phytic acid, allantoin, rose hip oil, PEG-40 hydrogenated castor oil, propylene glycol, tocopheryl acetate and bisabolol, adding into an emulsifying pot, heating to 80-85 ℃, stirring at constant temperature until the mixture is completely dissolved to obtain a material A, preserving the temperature of the material A for 10min, defoaming and cooling;

s2, dissolving the mint extract and the eucheuma extract in water to obtain a mixed solution I;

s3, when the temperature of the material A in the step S1 is reduced to 40-45 ℃, adding the cell growth factor composite liposome, the mixed liquid I in the step S2, the preservative and the essence, stirring and uniformly mixing, sampling and detecting, and discharging qualified products to obtain the compound liposome.

Comparative example 1

1) Preparing a deep-sea fish skin collagen zymolyte: the procedure of example 1 was otherwise the same as that of example 1, except that hydrolyzed cod skin protein was not added.

2) Preparation of cell growth factor complex liposome: the composition was supplemented with 0.02% by weight of hydrolyzed cod skin collagen of deep sea fish skin collagen hydrolysate, and the deep sea fish skin collagen obtained in this comparative example was subjected to enzymolysis, otherwise the same procedure as in example 3 was applied.

2) Preparation of post-basking repair gel containing cell growth factor: the cell growth factor complex liposome prepared in this comparative example was used, and the other examples were the same as example 6.

Comparative example 2

1) Preparation of cell growth factor complex liposome: the same procedure as in example 3 was repeated except that no extract of peanut sprouts was added.

2) Preparation of post-basking repair gel containing cell growth factor: the cell growth factor complex liposome prepared in this comparative example was used, and the other examples were the same as example 6.

Comparative example 3

Comparative example 3 differs from example 6 in that: eucheuma Gelatinosum extract is not added.

Examples of Effect test

1. And (3) testing a sample: the after-sun repair gels prepared in examples 4-6 and comparative examples 1-3.

2. The test method comprises the following steps: 180 volunteer subjects with healthy skin, no skin allergy history and sunburn symptoms are selected to participate in the test, wherein 118 women and 62 men are randomly divided into 6 groups according to sex, age and the like, 30 persons in each group respectively try the after-sun repair gel prepared in the embodiments 4-6 and the comparative examples 1-3, and during the trial period, the sunburn part is cleaned by water respectively in the morning, noon and evening, then a proper amount of after-sun repair gel is taken and applied to the sunburn part, and the trial period is 2 weeks. After the trial is finished, each volunteer evaluates the using effects of moisturizing and moisturizing the after-sun repair gel, rapidly relieving pain and itching (namely, swelling and pain and itching symptoms are obviously relieved within 5-20 min), rapidly fading the erythema (diffuse erythema is obviously faded or disappears within 3 h), preventing pigmentation (namely, no obvious suntan or only slight suntan), refreshing without greasy feeling, rapidly repairing the second-degree sunburn (namely, peeling symptoms are obviously improved or disappear within 4 days, blisters begin to scab and fall off), and the like, and scores 0-10 according to the using effects, 9-10 scores are good, 7-8 scores are good, 5-6 scores are general, 3-4 scores are poor, 0-2 scores are poor, and the average value is taken.

3. And (3) test results: see table 1.

TABLE 1 evaluation of the Effect of the inventive sunless repair gels containing cell growth factor

	Example 4	Example 5	Example 6	Comparative example 1	Comparative example 2	Comparative example 3
							Fresh and cool without greasy feeling	8.6	8.5	8.8	8.7	8.5	8.4
Moisturizing effect	7.8	8.2	8.5	7.6	7.3	7.1
							Quick analgesic and antipruritic effects	8.2	8.5	8.8	6.9	6.8	7.2
Quick regression effect of erythema	8.5	8.4	8.7	6.2	5.6	6.7
							Anti-pigmentation effect	8.4	8.6	8.9	6.4	5.8	6.8
Quick repair effect of second-degree sunburn	8.6	8.3	8.8	5.6	5.4	6.8

The test results in table 1 show that the use effect evaluation of the after-sun repair gel containing the cell growth factors prepared in embodiments 4 to 6 of the present invention is high, and the after-sun repair effect is significant. After the suncured repair gel containing the cell growth factors is used, the skin is fresh and clean without greasy feeling, the skin feeling is good, the moisturizing effect is obvious, the effects of quickly relieving pain and itching and eliminating erythema are obvious, meanwhile, the pigmentation can be relieved after the suncured repair gel is used, and the suncured repair gel also has obvious relieving and repairing effects on desquamation, blisters and other symptoms of second-degree sunburn.

From the results of comparative example 1, it is known that the deep sea fish skin collagen hydrolysate prepared by adding hydrolyzed cod skin protein in the preparation process contains more small molecular peptides, which is more beneficial to maintaining the activity of cell growth factors and improving the bioavailability of the cell growth factors, thereby significantly improving the relieving and repairing effects of the sunburn repairing gel on sunburn symptoms.

From the results of comparative example 2, it can be seen that the prepared suncured repair gel has more significant effects of alleviating and repairing sunburn symptoms by adding the cell growth factor complex liposome containing the peanut sprout extract in the components. The peanut sprout extract contains abundant vitamins, amino acids, trace elements and resveratrol, has obvious moisturizing and antioxidant effects, has a good protection effect on cell growth factors, and is beneficial to keeping the activity of the cell growth factors.

The results of comparative example 3 show that the after-sun repair gel containing no eucheuma extract has obviously reduced after-sun repair effect compared with example 6, which indicates that the after-sun repair gel containing cell growth factors has reasonable formula and synergistic effect among the components.

The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.

Claims (5)

1. The after-sun repair gel containing the cell growth factors is characterized by comprising the following components in percentage by weight: 1.2% of cell growth factor complex liposome, 2% of rose hip oil, 1.5% of phytic acid, 3% of mint extract, 5% of eucheuma extract, 9401.6% of carbomer, 0.1% of polyglutamic acid, 0.15% of allantoin, 3% of propylene glycol, 0.1% of PEG-40 hydrogenated castor oil, 0.4% of tocopherol acetate, 0.2% of bisabolol, 0.1% of preservative, 0.01% of essence and the balance of deionized water;

in the cell growth factor compound liposome, the cell growth factors are recombinant human basic fibroblast growth factor and recombinant human vascular endothelial growth factor;

the cell growth factor composite liposome comprises the following components in parts by weight: 0.002-0.006 part of recombinant human basic fibroblast growth factor, 0.001-0.002 part of recombinant human vascular endothelial cell growth factor, 2-10 parts of deep sea fish skin collagen zymolyte, 4-8 parts of peanut sprout extract, 80-120 parts of hydrogenated lecithin, 1-3 parts of sodium cholate and 20-30 parts of cholesterol;

the preparation method of the deep sea fish skin collagen zymolyte in the cell growth factor compound liposome comprises the following steps: adding water into deep sea fish skin collagen powder according to a solid-to-liquid ratio of 20-40 g/L, adding 0.01-0.05% of hydrolyzed cod skin protein by weight of the deep sea fish skin collagen powder, stirring for 15-30 min, adding 5-10% of compound protease by weight of the solution, carrying out enzymolysis for 5-8 h at the temperature of 45-55 ℃ and under the condition that the pH value is 6.0-7.0, carrying out enzyme deactivation at 100 ℃ for 5-10 min after the enzymolysis is finished, filtering, concentrating the filtrate under reduced pressure, and drying to obtain a deep sea fish skin collagen hydrolysate;

wherein the compound protease is composed of collagenase and bromelain according to the weight ratio of (4-10) to 1;

the preparation method of the peanut sprout extract comprises the following steps: taking peanuts, sterilizing, soaking seeds and accelerating germination, selecting peanut sprouts without fibrous roots and with root lengths of 0.2-1.5 cm, removing seed coats, homogenizing, adding water with the weight of 10-20 times of that of the peanut sprouts, carrying out ultrasonic extraction for 1-2 times at the temperature of 40-60 ℃, carrying out 30-60 min each time, filtering, combining filtrates, concentrating the filtrate under reduced pressure to 30-40% of the original volume, adding ethanol until the alcohol content is 70-80%, standing for 8-12 h at the temperature of 4-10 ℃, filtering, concentrating the filtrate under reduced pressure, and drying to obtain the peanut sprout extract.

2. The cell growth factor-containing post-basking repair gel of claim 1, wherein the preservative is housefly antimicrobial peptide.

3. The postsun repair gel containing a cell growth factor according to claim 1, wherein the preparation method of the cell growth factor complex liposome comprises the following steps:

1) adding hydrogenated lecithin, sodium cholate and cholesterol into absolute ethyl alcohol according to the solid-to-liquid ratio of 2-5 mg/mL, and stirring for dissolving to obtain a solution I;

2) adding PBS into the recombinant human basic fibroblast growth factor, the recombinant human vascular endothelial cell growth factor, the deep-sea fish skin collagen hydrolysate and the peanut sprout extract according to the solid-to-liquid ratio of 4-8 mg/mL, and stirring and dissolving to obtain a solution II;

3) adding the solution I obtained in the step 1) and the solution II obtained in the step 2) into a supercritical autoclave, wherein the temperature is 30-40 ℃, the pressure is 20-30 MPa, and CO is added₂Emulsifying for 30-45 min under the condition of the flow rate of 12-16L/h, and separating an emulsified product under reduced pressure to obtain a cytokine complex liposome; wherein the separation temperature is 30-40 ℃, and the separation pressure is 5-15 MPa.

4. The method for preparing the cell growth factor-containing postsun repair gel according to claim 3, wherein in the step 2), the pH of the PBS is 7.2-7.6.

5. The method for preparing the cell growth factor-containing gel for post-basking repair according to any one of claims 1 to 4, comprising the steps of:

s1, adding deionized water into carbomer 940 and polyglutamic acid, dispersing and swelling with phytic acid, allantoin, rose hip oil, PEG-40 hydrogenated castor oil, propylene glycol, tocopheryl acetate and bisabolol, adding into an emulsifying pot, heating to 80-85 ℃, stirring at constant temperature until the mixture is completely dissolved to obtain a material A, preserving the temperature of the material A for 10-15 min, defoaming and cooling;

s2, dissolving the mint extract and the eucheuma extract in water to obtain a mixed solution I;

s3, when the temperature of the material A in the step S1 is reduced to 40-45 ℃, adding the cell growth factor composite liposome, the mixed liquid I in the step S2, the preservative and the essence, stirring and uniformly mixing, sampling and detecting, and discharging qualified products to obtain the compound liposome.