CN108184528A - A kind of method of ginkgo with heel cuttage - Google Patents
A kind of method of ginkgo with heel cuttage Download PDFInfo
- Publication number
- CN108184528A CN108184528A CN201810051673.8A CN201810051673A CN108184528A CN 108184528 A CN108184528 A CN 108184528A CN 201810051673 A CN201810051673 A CN 201810051673A CN 108184528 A CN108184528 A CN 108184528A
- Authority
- CN
- China
- Prior art keywords
- cuttage
- cuttings
- ginkgo
- cut
- heel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Abstract
The invention discloses a kind of method of ginkgo with heel cuttage, include the following steps：(1) it in early or mid May, when ginkgo current-year branch is up to more than 10cm, is cut at shoot and old branch junction lower part 2cm, retains shoot length in 10 12cm, the length of old branch upper and lower part is 1cm, as band heel cuttings；10 12cm long will be cut into heel cuttings, and be cut into flat nick, and retain blade at 1.0cm above the bud, lower cut is cut into single horse ear shape；(2) cuttings lower part is put into ABT root-inducing powders before cuttage and impregnates 2h, first with bamboo stick in cuttage Chi Neichadong during cuttage, cuttings is inserted into hole again, face of blade is upward, cuttage depth is 4~6cm, and seeding row spacing is 10 × 30cm, is then gently compacted matrix, and sprinkle profoundly water immediately, matrix is made to combine closely with cuttings.The present invention can effectively solve the age effect of ginkgo cutting propagation, improve the rooting rate of ginkgo cuttage, reduce seedling cost, be conducive to accelerate the breeding speed of Seedlings of Ginkgo biloba and keep the good characteristic of kind.
Description
Technical field
The present invention relates to arboreal growth development and reproduction technique fields, and in particular to a kind of method of ginkgo with heel cuttage.
Background technology
Ginkgo (Ginkgo biloba L.) is Ginkgoaceae Mono-species genus, is that quaternary glacier uniquely preserves later
Relict Plant, " living fossil " being referred to as in plant kingdom is one of seeds very precious in the world.Ginkgo collection flower, fruit,
The values such as leaf, material, ornamental plantation, have higher economic benefit, ecological benefits and social benefit, paleobotany,
Plant Taxonomy, plant comparative morphology, phytotomy, embryology and pomology etc. have very high scientific research valency
Value.Ginkgo is high flavones and terpene lactones plant, ginkgo biloba p.e to the cerebrovascular and angiocardiopathy, improve immunity of organisms,
Reducing blood glucose and cholesterol etc. has obvious curative effects；Food containing gingko almond has the effects that important health care, slows down aging.
There are many modes of ginkgo breeding, and wherein cuttage and seedling culture can promote early solid, how solid, and it is excellent to accelerate ginkgo
The quick breeding of non-defective unit kind, while filial generation can also keep the excellent hereditary capacity on maternal plant.At present, the mode of ginkgo cuttage and seedling culture
Mainly there are an epicormic branch cuttage and cuttage, but since taking root for ginkgo cuttings needs the differentiation by callus and complete, it is raw
Root needs the time long, slow-growing, and cuttage survival rate is relatively low；Moreover, the radical reduction with the increase at age of ginkgo cuttage root-taking,
" age effect " has become where the difficult point for restricting ginkgo cutting propagation.
Invention content
For the above-mentioned prior art, it the object of the present invention is to provide a kind of method of ginkgo with heel cuttage, can significantly carry
The slotting cuttage survival rate of height, solves the problems, such as that ginkgo cutting propagation survival rate is low in production practices.
To achieve the above object, the present invention adopts the following technical scheme that：
The first aspect of the present invention provides a kind of method of ginkgo with heel cuttage, includes the following steps：
(1) preparation of cuttings：In early or mid May, when ginkgo current-year branch is up to more than 10cm, from shoot and old branch
It is cut at the 2cm of junction lower part, retains shoot length in 10-12cm, the length of old branch upper and lower part is 1cm, is inserted as band heel
Fringe；10-12cm long will be cut into heel cuttings, and be cut into flat nick, and retain blade at 1.0cm above the bud, lower cut is cut into
Single horse ear shape；
(2) cuttage：Cuttings lower part is put into ABT root-inducing powders before cuttage and impregnates 2h, first with bamboo stick in cuttage pond during cuttage
Interpolation hole, then cuttings is inserted into hole, upward, cuttage depth is 4~6cm to face of blade, and seeding row spacing is 10 × 30cm, then will
Matrix is gently compacted, and is sprinkled profoundly water immediately, and matrix is made to combine closely with cuttings.
Preferably, in step (1), ancient ginkgo of the ginkgo for the age of tree 100 years or more.
Preferably, in step (2), a concentration of 50mg/L of the ABT root-inducing powders.
Preferably, in step (2), the cuttage pond is located in vinyl house of the southern exposure with automatic intermittent spraying system,
Covering sunshade net at the top of greenhouse after cuttage bottom of pond portion is smooth, spreads the stone of 10cm thickness, the wide 1.2m in seedbed, seedbed spacing 80cm,
Depth 50cm sets among seedbed a height for 80cm, is divided into the spraying nozzle of 1.2m.
Preferably, in step (2), the matrix is 1 in mass ratio by clinker and sand:3 mix.
Further, the above method further includes：Management after cuttage.
Management after the cuttage includes：Temperature is controlled at 22~27 DEG C, control humidity is every after 85%-95%, cuttage
It carries out disinfection every the carbendazim solution of spray one time 0.5% in 7 days to matrix, after cuttage rooting, weekly with 1 ‰ urea or 2 ‰
Potassium dihydrogen phosphate carry out foliage application.
Beneficial effects of the present invention：
(1) cuttage that the present invention will be applied to ginkgo with heel cuttage technique for the first time, can effectively solve ginkgo cutting propagation
Age effect improves the rooting rate of ginkgo cuttage, reduces seedling cost, be conducive to accelerate Seedlings of Ginkgo biloba breeding speed and
Keep the good characteristic of kind.
(2) method of ginkgo of the invention with heel cuttage is applicable not only to the cutting propagation of the smaller Seedlings of Ginkgo biloba of seedling age,
But also especially suitable for the cutting propagation of ancient ginkgo, method using the present invention carries out ancient ginkgo cutting propagation, cuttings life
Root situation is obviously improved, and cuttage survival rate significantly improves, easy to operate, of low cost, solves ginkgo cuttage since " age imitates
Should " caused by nursery it is difficult to take root the problem of, improve ginkgo breeding breeding and promote efficiency.
Description of the drawings：
Fig. 1 is cuttings lower cut side schematic view；
Fig. 2 is cuttings lower cut front schematic view；
Fig. 3 forms callus schematic diagram for cuttings lower cut；
Fig. 4 is cuttage rooting schematic diagram.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.It is unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
As described in background technology, ginkgo belongs to callus rooting type, belongs to seeds more difficult to take root, and
And also there are age effects for ginkgo cutting propagation, the rootability of cuttings is as the age of elite stand increases and reduces, therefore, now
The fringe bar that some ginkgo cutting propagations were generally all acquired from 7 years on raw following elite stand, for the silver of the elite stand age of tree 10 years or more
Apricot cuttage and seedling culture is less to be had been reported that.Based on this, the present invention provides a kind of new ginkgo cuttage and seedling culture methods, will be with heel cuttage skill
Art is applied to the cuttage and seedling culture of ginkgo, and using a part of old branch carried with heel cuttings lower part, tissue tight easily takes root,
Improve the cuttage survival rate of ginkgo.
In one embodiment of the present invention, the ginkgo provided with heel cuttage method be：
(1) cuttage seedbed preparation：
Cuttage pond is located in vinyl house of the southern exposure with automatic intermittent spraying system, covering sunshade net, skewer at the top of greenhouse
After slotting bottom of pond portion is smooth, the stone of 10cm thickness, the wide 1.2m in seedbed, seedbed spacing 80cm, depth 50cm are spread, seedbed centre is set
One height is 80cm, is divided into the spraying nozzle of 1.2m.Husky (ratio 1 is mixed with clinker:3, i.e. clinker and sand is 1 in mass ratio:
3 mixing) it is cutting medium.
(2) preparation of cuttings
In early or mid May, current-year branch is cut when 10cm or so at shoot and old branch junction lower part 2cm,
Retain shoot length in 10~12cm, each long 1cm in old branch top and the bottom, as band heel cuttings, 10~12cm will be cut into heel cuttings
It is long, flat nick is cut into, and retain blade at 1.0cm above the bud, lower cut is cut into single horse ear shape, it is first horizontal with sharp blade
To incision, then rip cutting vertically downward, keep notch smooth, it is every 50 a bundle, tie spare.
(3) cuttage
Cuttings carries out cuttage after being ready to, and the liquor potassic permanganate that mass concentration is 0.1% will be sprayed in cuttage pond and is disappeared
Poison, the ABT root-inducing powders that cuttings lower part is put into 50mg/L before cuttage impregnate 2h, first with bamboo stick in cuttage Chi Neichadong during cuttage,
Cuttings is inserted into hole again, upward, cuttage depth is 4~6cm to face of blade, and seeding row spacing is 10 × 30cm, then that matrix is light
Light compacting, and sprinkle profoundly water immediately, matrix is made to combine closely with cuttings.
(4) it is managed after cuttage
In one layer of sunshade net of greenhouse upper cover after cuttage, control temperature is at 22~27 DEG C, and humid control is 90% or so, cuttage
Carry out disinfection afterwards every the carbendazim solution of one time 0.5% of spray in 7 days to matrix, after cuttage rooting, weekly with 1 ‰ urea or
2 ‰ potassium dihydrogen phosphate carries out foliage application.
In the above method, the selection of the preparation for ginkgo cuttage seedbed, particularly cutting medium is for rooting of cuttings
Influence it is very big, the present invention selection is optimized to the composition of cutting medium, as a result, it has been found that, select clinker and sand in mass ratio for
1:3 mixing can effectively keep moisture, prevent cuttage position by pathogenic bacterial infection, while promote cuttings as cutting medium
It takes root.
Selection for cuttings, since ginkgo is callus rooting type, and cutting propagation is there are age effect, specifically
Efficiency decline of taking root is shown as, rooting effect is degenerated, very big especially for the difficulty of the cutting propagation of ancient ginkgo.The present invention
It will be applied to the cutting propagation of ginkgo with heel cuttings for the first time, compared with the methods of epicormic branch cuttage and cuttage, using band heel skewer
The rooting rate and survival rate of ancient ginkgo cuttage can be significantly improved by inserting.
For the management after ginkgo cuttage, control of temperature and humidity is related generally to, temperature is excessively high, humidity is excessive, can cause to insert
Item take root disease rot；Temperature, humidity are too low, then the death that can lead to that rooting of cuttings is slow, cutting is dried up.The present invention passes through to temperature
The optimum choice of degree, damp condition improves the survival rate of ginkgo cutting propagation.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.
Test material used in the embodiment of the present invention and comparative example is the test material of this field routine, can be passed through
Commercial channel is commercially available.
The embodiment of the present invention and comparative example are carried out in Shandong Province Tai'an Shandong Agricultural University's Ginkgo Germplasm Resource garden
's.
Embodiment 1：
Growth selection is vigorous, no disease and pests harm, and normotrophic 8 years raw ginkgo seedlings are the subjects of cutting propagation.
Cuttage step is as follows：
(1) cuttage seedbed preparation：
Cuttage pond is located in vinyl house of the southern exposure with automatic intermittent spraying system, covering sunshade net, skewer at the top of greenhouse
After slotting bottom of pond portion is smooth, the stone of 10cm thickness, the wide 1.2m in cuttage pond, spacing 80cm, depth 40cm, seedbed centre setting one are spread
Item height is 80cm, is divided into the spraying nozzle of 1.2m.Husky (ratio 1 is mixed with clinker:3) it is cutting medium.
(2) preparation of cuttings
In early or mid May, current-year branch is cut when 10cm or so at shoot and old branch junction lower part 2cm,
Retain shoot 10cm, each long 1cm in old branch top and the bottom, as band heel cuttings, 10cm long will be cut into heel cuttings, above bud
Flat nick is cut at 1.0cm, and retains leaf, lower cut is cut into single horse ear shape, is first cut transversely into sharp blade, then hang down
Straight downward rip cutting keeps notch smooth, it is every 50 a bundle, tie spare.
(3) cuttage
Cuttings carries out cuttage after being ready to, and the liquor potassic permanganate that mass concentration is 0.1% will be sprayed in cuttage pond and is disappeared
Cuttage is carried out after poison, the ABT root-inducing powders that cuttings lower part is put into 50mg/L before cuttage impregnate 2h, first with bamboo stick in cuttage during cuttage
Chi Neichadong, then cuttings is inserted into hole, upward, cuttage depth is 6cm to face of blade, and seeding row spacing is 10 × 30cm, then will
Matrix is gently compacted, and is sprinkled profoundly water immediately, and matrix is made to combine closely with cuttings.
(4) cuttage post processing and management
In one layer of sunshade net of greenhouse upper cover after cuttage, control temperature is at 22~27 DEG C, and humid control is 90% or so, cuttage
Carry out disinfection afterwards every the carbendazim solution of one time 0.5% of spray in 7 days to matrix, after cuttage rooting, weekly with 2 ‰ di(2-ethylhexyl)phosphate
Hydrogen potassium carries out foliage application.
Embodiment 2：
Subjects of the ancient ginkgo for selecting life in 100 years above for cutting propagation.Cuttage step is the same as embodiment 1.
In order to which the effect of the present invention is better described, following contrast experiment is carried out.
Comparative example 1：
Subjects of the ancient ginkgo for selecting life in 100 years above for cutting propagation.The preparation in cuttage seedbed, cuttage, skewer
Processing and management after inserting with embodiment 1, difference lies in：The preparation method of cuttings is：
Clip ginkgo current year raw spray, the long 15-20cm of cuttings, 3-4 bud in every cuttings, cuttings upper cut is above bud
At 1cm, notch is rounded for flat nick, and lower cut, in horse ear shape, retains the blade in cuttings away from nearest bud 0.5-1cm.
Comparative example 2：
Subjects of the ancient ginkgo for selecting life in 100 years above for cutting propagation.The preparation in cuttage seedbed, cuttage, skewer
Processing and management after inserting with embodiment 1, difference lies in：The preparation method of cuttings is：
The band leaf portion point of the one of clip ginkgo biennial ripe branch, retains a leaf and leaf bud, with leaf after mid-May
And retain 1-3cm to both sides clip, branch centered on leaf bud, the negative direction one side for having leaf bud on the branch after clip with cutter
Branch skin is longitudinally scratched, branch skin is connected leaf bud with hand peels together, takes out xylem.
Comparative example 3：
Subjects of the ancient ginkgo for selecting life in 100 years above for cutting propagation.The preparation in cuttage seedbed, cuttage, skewer
Processing and management after inserting with embodiment 1, difference lies in：The preparation method of cuttings is：
Cuttage begins preparing for cuttings after current year bud dormancy, blocks branch at the 1cm of bud top, notch is truncation
Mouthful, lower section should be blocked at 1cm below bud, and smooth single horse ear shape is whittled into sharp blade, length using 15~20cm as
Preferably, every 50 cuttings, a bundle of sand storage preserves after interception.
Comparative example 4：
Subjects of the ancient ginkgo for selecting life in 100 years above for cutting propagation.It will be replaced with for the matrix of cuttage
Perlite, yellow mud detritus and sand in mass ratio 2:1:2 mixing, remaining is the same as embodiment 1.
The Seedlings of Ginkgo biloba strain number of embodiment 1 and comparative example 1-4 is identical, counts the rooting rate and survival rate of each group nursery stock.Knot
Fruit is shown in Table 1.
Table 1：Each group rooting rate and survival rate statistical result
The foregoing is merely the preferred embodiments of the application, are not limited to the application, for the skill of this field
For art personnel, the application can have various modifications and variations.It is all within spirit herein and principle, made any repair
Change, equivalent replacement, improvement etc., should be included within the protection domain of the application.
Claims (7)
1. method of a kind of ginkgo with heel cuttage, which is characterized in that include the following steps：
(1) preparation of cuttings：In early or mid May, when ginkgo current-year branch is up to more than 10cm, combined from shoot with old branch
It is cut at place lower part 2cm, retains shoot length in 10-12cm, the length of old branch upper and lower part is 1cm, is called in person cuttings as band；
10-12cm long will be cut into heel cuttings, and be cut into flat nick, and retain blade at 1.0cm above the bud, lower cut is cut into single horse
Ear shape；
(2) cuttage：Cuttings lower part is put into ABT root-inducing powders before cuttage and impregnates 2h, first with bamboo stick in cuttage pond interpolation during cuttage
Hole, then cuttings is inserted into hole, upward, cuttage depth is 4~6cm to face of blade, and seeding row spacing is 10 × 30cm, then by matrix
Compacting, and sprinkle profoundly water immediately, matrix is made to combine closely with cuttings.
2. according to the method described in claim 1, it is characterized in that, in step (1), the ginkgo is the age of tree 100 years or more
Ancient ginkgo.
3. according to the method described in claim 1, it is characterized in that, in step (2), a concentration of 50mg/ of the ABT root-inducing powders
L。
4. according to the method described in claim 1, it is characterized in that, in step (2), the cuttage pond is located at southern exposure with automatic
In the vinyl house of intermittent spraying system, covering sunshade net, after cuttage bottom of pond portion is smooth, spreads the stone of 10cm thickness at the top of greenhouse
Son, the wide 1.2m in seedbed, seedbed spacing 80cm, depth 50cm set among seedbed a height for 80cm, are divided into the spray of 1.2m
Fog spray head.
5. according to the method described in claim 1, it is characterized in that, in step (2), the matrix presses quality by clinker and sand
Than being 1:3 mix.
6. it according to the method described in claim 1, it is characterized in that, further includes：The step of being managed after cuttage.
7. according to the method described in claim 6, include it is characterized in that, being managed after the cuttage：Temperature is controlled 22~27
DEG C, control humidity carries out disinfection to matrix every the carbendazim solution of spray one time 0.5% in 7 days after 85%-95%, cuttage, treats
After cuttage rooting, foliage application is carried out with 1 ‰ urea or 2 ‰ potassium dihydrogen phosphate weekly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810051673.8A CN108184528B (en) | 2018-01-19 | 2018-01-19 | Method for heel-carrying cuttage of ginkgo biloba |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810051673.8A CN108184528B (en) | 2018-01-19 | 2018-01-19 | Method for heel-carrying cuttage of ginkgo biloba |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108184528A true CN108184528A (en) | 2018-06-22 |
| CN108184528B CN108184528B (en) | 2020-05-19 |
Family
ID=62589909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810051673.8A Expired - Fee Related CN108184528B (en) | 2018-01-19 | 2018-01-19 | Method for heel-carrying cuttage of ginkgo biloba |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108184528B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114027086A (en) * | 2021-12-20 | 2022-02-11 | 曹文玉 | Ginkgo cutting propagation seedling raising method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181286A (en) * | 2013-03-13 | 2013-07-03 | 山东农业大学 | Method for overcoming topophysis in vegetative propagation of gingkoes |
| CN105409955A (en) * | 2015-11-24 | 2016-03-23 | 上海园林工程设计有限公司 | Reagent promoting growth of plant root systems and preparation method of reagent |
-
2018
- 2018-01-19 CN CN201810051673.8A patent/CN108184528B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181286A (en) * | 2013-03-13 | 2013-07-03 | 山东农业大学 | Method for overcoming topophysis in vegetative propagation of gingkoes |
| CN105409955A (en) * | 2015-11-24 | 2016-03-23 | 上海园林工程设计有限公司 | Reagent promoting growth of plant root systems and preparation method of reagent |
Non-Patent Citations (2)
| Title |
|---|
| 王惠珍等: "银杏嫩枝扦插育苗技术试验", 《中国园艺文摘》 * |
| 苏淑钗: "银杏的嫩枝扦插", 《农技服务》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114027086A (en) * | 2021-12-20 | 2022-02-11 | 曹文玉 | Ginkgo cutting propagation seedling raising method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108184528B (en) | 2020-05-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101142889B (en) | High-yield cultivating method for winter jujube in south | |
| CN108094175B (en) | Hazelnut twig cutting seedling method | |
| CN103238400B (en) | Method for removing seed dormancy of tapiscia sinensis oliv and improving seedling survival rate | |
| CN102177795B (en) | Cuttage and seedling raising method for clematis hybrids | |
| CN104429543A (en) | Cutting-seedling raising method of cyclocarya paliurus branches | |
| CN107306741B (en) | Cultivation method of aquilaria sinensis nursery stock | |
| CN100571503C (en) | A kind of elaeagnus mollis asexual reproduction method | |
| CN104145707A (en) | Method for cultivating seedlings by means of green branches under full exposure and light mist condition | |
| CN101849505A (en) | Method for inducing embryonal-suspensor mass (ESM) regeneration plant from immature seed of pinus massoniana | |
| CN108605537A (en) | A kind of method of purplefruit holly leaf epicormic branch cutting | |
| CN102630544A (en) | Method for planting Picea koraiensis | |
| CN106034722A (en) | Cutting propagation method of golden leaf plant namely weigela florida variegata | |
| CN106212172A (en) | Poem beautiful jade leads to cuttage and seedling culture method with a smile | |
| CN108718740A (en) | Using mulch to the method for golden Chinese scholartree cuttage and seedling culture | |
| CN108307911A (en) | A kind of root cutting growth method of barna | |
| CN104813938A (en) | Panax notoginseng tissue culture seedling raising method | |
| CN113785706A (en) | Cutting seedling method of Siberian apricot trees | |
| CN109220261A (en) | A kind of dove tree seedling breeding method | |
| CN105993790A (en) | White tea hardwood branch cutting seedling method | |
| CN105815184A (en) | Color-leafed plant cutting propagation method | |
| CN103703997B (en) | Reproduction method for improving eucalyptus cutting rooting rate and scion strip utilization rate | |
| CN108142126A (en) | A kind of cuttage and seedling culture method of heavy water camphor tree | |
| CN108184528A (en) | A kind of method of ginkgo with heel cuttage | |
| CN112021168A (en) | Method for planting dendrobium officinale by using straw ropes to simulate wild trees | |
| CN109302902A (en) | A kind of apocarya grafting implantation methods |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200519 Termination date: 20220119 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |