CN108179013A - A kind of alkaline land soil biological modification agent and preparation method thereof - Google Patents
A kind of alkaline land soil biological modification agent and preparation method thereof Download PDFInfo
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- 239000002689 soil Substances 0.000 title claims abstract description 118
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 77
- 238000012986 modification Methods 0.000 title claims abstract description 35
- 230000004048 modification Effects 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 235000010958 polyglycerol polyricinoleate Nutrition 0.000 claims abstract description 50
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 230000001580 bacterial effect Effects 0.000 claims abstract description 36
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000004021 humic acid Substances 0.000 claims abstract description 10
- 239000011159 matrix material Substances 0.000 claims abstract description 10
- 241000589516 Pseudomonas Species 0.000 claims description 20
- 238000004321 preservation Methods 0.000 claims description 20
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 13
- 244000005700 microbiome Species 0.000 claims description 13
- YXOLAZRVSSWPPT-UHFFFAOYSA-N Morin Chemical compound OC1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 YXOLAZRVSSWPPT-UHFFFAOYSA-N 0.000 claims description 10
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
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- 108010080698 Peptones Proteins 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
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- 229910019142 PO4 Inorganic materials 0.000 description 3
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- 238000011161 development Methods 0.000 description 3
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- 235000021317 phosphate Nutrition 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
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- 239000011780 sodium chloride Substances 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
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- 238000002156 mixing Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
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- 244000000000 soil microbiome Species 0.000 description 2
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- 108091000130 1-aminocyclopropane-1-carboxylate deaminase Proteins 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102100035882 Catalase Human genes 0.000 description 1
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- 108010046334 Urease Proteins 0.000 description 1
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- 150000001413 amino acids Chemical class 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000004178 biological nitrogen fixation Methods 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
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- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
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- 210000003608 fece Anatomy 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 230000015784 hyperosmotic salinity response Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
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- 239000010871 livestock manure Substances 0.000 description 1
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- 150000007524 organic acids Chemical class 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002686 phosphate fertilizer Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 241000873303 rhizosphere bacterium Species 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
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- 230000003248 secreting effect Effects 0.000 description 1
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- 230000007928 solubilization Effects 0.000 description 1
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- 239000008223 sterile water Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 239000001038 titanium pigment Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The present invention relates to a kind of alkaline land soil biological modification agents and preparation method thereof.It is uniformly mixed to prepare by the component of following parts by weight content：Matrix 100, PGPR liquid bacterial agents 1 80 120, PGPR liquid bacterial agents 280 120, humic acid 0.05 0.2, the bacteria containing amount of PGPR liquid bacterial agents 1,2 is (0.5 1.5) × 108CFU/ml.Salinized soil is improved using modifying agent of the present invention, soil physico-chemical property can be effectively improved, enhances biological activity of soil, discharges soil fertility, and environmental-friendly, preparation method processing step is simple, is suitble to industrialized production.
Description
Technical field
The present invention relates to alkaline land improving fields, and in particular to a kind of alkaline land soil biological modification agent.
Background technology
Salinization soil is widely present all over the world, and China salt marsh soil is even more that area is big and distribution is wide, from the Pacific Ocean
, from Hainan Island to Hulun Buir Sandy Land plateau, there is salination in the littoral shore of Dong Hai everywhere to the Tarim Basin in Xinjiang
SOIL DISTRIBUTION.According to Ministry of Agriculture's Second National census statistics, China's salinized soil gross area is about 5.2 hundred million mu, wherein opened up wasteland and
It it is 8652.58 ten thousand mu as arable land.Warming for global climate has been further exacerbated by arid, semiarid zone soil salinization journey
Degree, causes salinization soil area constantly to expand, and severe salinization soil shows a rising trend, and the alkaline spot area of no utility value expands
Greatly.Northwest, North China and Northeast China region and it is coastal be China's salinized soil main integrated distribution area.
The conventional method of improving saline soil mainly has water resources industry, physical measure, chemical measure and plant measures.Closely
Nian Lai, the research of microbial method improving salinization soil are constantly strengthened, and beneficial microbe can particularly promote plant to give birth to
Long bacterium has very great help to growth of the plant in salinization soil.Many microorganisms can be direct or indirect promotion plant
Object is grown, and the pressure of enhancing plants against organisms stress and abiotic stress, some researches show that plant growth-promoting rhizobacteria (plant
Growth promoting rhizobacteria, PGPR) influence of the salt stress to different plants can be alleviated and can be increased
The salt tolerance of strong plant.
Existing alkaline land improving technology is big there is quantities, and of high cost, effect duration is short, needs constantly to tie up
Shield has soil the problems such as destructive, and improvement is limited by various environmental factors, therefore studies a kind of novel salt-soda soil
Improving technology is just particularly important.
Plant growth-promoting rhizobacteria by two ways promote plant growth, respectively directly act on and indirectly-acting.Directly
The growth-promoting functions connect, which refer to, can synthesize certain compounds for plant utilization or plant is contributed to absorb certain nutrients from environment,
Including in biological nitrogen fixation, dissolving soil Phos, synthesize some plant hormones (such as growth hormone) and the enzyme needed for plant,
Such as acc deaminase；And indirectly growth-promoting functions refer to the biological control to pathogenic microorganism, can mitigate or avoid a certain
The illeffects of kind or several pathogenic microorganisms, including generating antibiotic, secretion siderophore, competition ecological niche and induction plant
Generate resistance etc..
How much microorganism is soil fertility or an index in salt-soda soil.Salt-soda soil micro organism quantity is few, is due to microorganism
Profitable strain is not easy existence procreation under alkaline condition.Main cause is to be unfavorable for microbe survival under saline-alkali environment, not micro-
Biology does not just convert organic matter, so as to the raw bioprotein and amino acid not needed into plant.It only increases significantly saline and alkaline
Microorganism system in soil could improve salt-soda soil, activate organic matter, improve plant survival rate.Organic matter in soil is cured
More, the more, soil productivity is better for profitable strain microorganism.
Invention content
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide one kind can be effectively improved
Soil physico-chemical property, enhancing biological activity of soil, discharge soil fertility, and the environmental-friendly soil improvement suitable for salt-soda soil
Agent and preparation method, the preparation method processing step is simple, is suitble to industrialized production.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of alkaline land soil biological modification agent is uniformly mixed to prepare by the component of following parts by weight content：
The bacteria containing amount of the PGPR liquid bacterial agents 1 is (0.5-1.5) × 108CFU/ml；
The bacteria containing amount of the PGPR liquid bacterial agents 2 is (0.5-1.5) × 108CFU/ml。
Preferably, a kind of alkaline land soil biological modification agent uniformly mixes system by the component of following parts by weight content
：
The bacteria containing amount of the PGPR liquid bacterial agents 1 is 1 × 108CFU/ml；
The bacteria containing amount of the PGPR liquid bacterial agents 2 is 1 × 108CFU/ml。
Contained PGPR is bacillus amyloliquefaciens S3-1 (Bacillus in the PGPR liquid bacterial agents 1
AmyloliquefaciensS3-1), deposit number is CCTCC AB 2014337；The preservation time：On December 12nd, 2014；
Depositary institution：China typical culture collection center (China Center for Type CultureCollection), referred to as
CCTCC；Preservation address：(the first attached primary school pair of Wuhan University in the school of No. 299 Wuhan Universitys of Wuhan City, Hubei Province Wuchang District Bayi Road
Face), Wuhan University's collection.
Contained PGPR is Pseudomonas aurantica JD37 (Pseudomonas in the PGPR liquid bacterial agents 2
AurantiacaJD37), deposit number is CGMCC No.1.10967；The preservation time：In May, 2011；Depositary institution：China
Microbiological Culture Collection administration committee common micro-organisms center (China General Microbiological Culture
Collection Center), abbreviation CGMCC；Preservation address：The Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences
Institute of microbiology.
The matrix is commercially available turf, chooses the particle that grain size is 0.35-1.4mm, adjusts its pH value as 6.5-7.5.
The mass content of chloride in the humic acid is less than or equal to 0.05%, and the mass content of sulfate is less than etc.
In 0.05%, loss on drying is less than or equal to 2.0%, and ignition residue is less than or equal to 0.5%.
The preparation method of the alkaline land soil biological modification agent, includes the following steps：
(1) matrix pre-processes：It is in mass ratio by calcium carbonate (0.5-2)：9 add in turfy soil, adjust its pH value as 6.5-
7.5, by turfy soil pulverize it is last vibrated a diameter of 0.35mm and 1.4mm standard screen choose grain size be 0.35-1.4mm
Particle, be dried for standby after sterilizing；
(2) PGPR liquid bacterial agents are prepared：By bacillus amyloliquefaciens S3-1 (Bacillus
AmyloliquefaciensS3-1 it) is inoculated with respectively with Pseudomonas aurantica JD37 (Pseudomonas aurantiacaJD37)
Fluid nutrient medium after sterilizing, culture obtain PGPR liquid bacterial agents 1 and PGPR liquid bacterial agents 2；
(3) it mixes：PGPR liquid bacterial agents 1 and PGPR liquid bacterial agents 2 are mixed and stirred in matrix uniformly, after natural air drying
The humic acid of powdery is added in, is stirred for uniformly, obtaining alkaline land soil biological modification agent.
The sterilizing sterilizes 20-30min at a temperature of being 121 DEG C, and number is once or twice.
The fluid nutrient medium be LB culture mediums, the LB liquid medium by every liter of deionized water add in 10g egg
It is stirred evenly after white peptone, 5g yeast extracts and 10gNaCl obtained.
Bacillus amyloliquefaciens S3-1 (Bacillus amyloliquefaciensS3-1) and orange vacation in step (2)
The culture of monad JD37 (Pseudomonas aurantiacaJD37) is the shaken cultivation under conditions of 28 DEG C, 200r/min
20 hours.
Plant growth-promoting rhizobacteria (plant growth promoting rhizobacteria, PGPR) is to refer to promote
Plant can promote the absorption and utilization or generation of mineral matter nutritional the metabolin of plant growth or even inhibit harmful micro-
Biology, it can directly or indirectly promote the beneficial rhizosphere bacterium of plant growth.In plant growth-promoting bacteria, there is phosphate solubilization
Bacterium and the bacterium of auxin can be secreted occupy important proportion.Phosphorus is only second to a large amount of of nitrogen necessary to be plant
Element, but after phosphate fertilizer is manured into soil, 75% is converted to insoluble Forms of Phosphate, it is impossible to be absorbed by plants and utilize.Salt marsh
In soil, insoluble phosphate is mainly the phos-phate forms of calcium, and Soluble phosphorus microorganism can dissolve soil by secreting organic acid
The phosphorus of middle slightly solubility including Phos and organophosphor, improves the content of titanium pigment in soil, so as to improve plant to P elements
Absorption.
Humic acid is apparent to the improving effect of soil, can improve the crumb structure of soil, can breathe freely, heat and
Water storage promotes growth and development of plants, improves stress resistance of plant.Humic acid material can provide sufficient C, N element supply soil
Microorganism so as to promote microbial metabolism and growth and development, increases soil microbial biomass, promotes plant and microorganism growth.It is rotten
It grows acid to flocculate in soil, the soil particle cementation of dispersion can be got up, the good crumb structure of water stability is formed, so as to promote
The formation of soil agreegate provides good condition for plant root growth development.Turf contains large quantity of moisture, humic acid and not
Plant residue, humus and a part of minerals thoroughly decomposed, (foreign countries think to surpass the content of organic matter more than 30%
It crosses 50%), it is environmentally friendly.Turf is also simultaneously good microbial adsorbent, can effectively adsorb microorganism, keeps microorganism
Activity.The matrix and humic acid of different content play the role of growth and the activity of microorganism closely related.Turf adsorbs
The ability of thalline reduces with the increase of diameter, but due to containing a large amount of nutritional ingredient in zymotic fluid, and preservation after a week will
A large amount of miscellaneous bacterias are generated, therefore the turf of a diameter of 0.35-1.4mm is used to prepare modifying agent.
This alkaline land soil biological modification agent has used two kinds of bacterial strains to be combined, and wherein bacillus amyloliquefaciens S3-1 is to tool
There are a soil remediation and Plant growth promotion, Pseudomonas aurantica JD37 also has a Plant growth promotion, and two kinds strain-combined to have
The vigor and the improved effect to soil of effect enhancing both sides, and promote plant growth.If a certain ingredient is excessive or crosses major general not
It can achieve the effect that the present invention.
Field can be applied to when using using the present invention as base manure.After alkaline land soil biological modification agent of the present invention,
Alkaline land soil pH value and EC values can be effectively reduced, reduces murder by poisoning of the high salinity to vegetation.Alkaline land soil biology refers to
Mark be improved significantly.Edaphon is as the degradation person of organic matter and the active library of plant nutrient substance, in soil nutrient
In play an important role, it is closely related with soil quality.After saline-alkali land soil conditioner, soil bacteria, fungi, unwrapping wire
Bacterium number amount increases, and alkaline land soil fertility is kept, raising substance cycle and raising bio-diversity have great importance.
The PGPR bacterial strains of the present invention can effectively improve salinized soil, promote plant growth.This product production technology
Simply, it is nontoxic to environment, it is environmentally friendly.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
A kind of alkaline land soil biological modification agent is uniformly mixed to prepare by the component of following parts by weight content：
Specific implementation step is as follows：
(1) matrix pre-processes：By calcium carbonate according to mass ratio 1:9, which add in ground turfy soil powder, adjusts its pH value extremely
Turfy soil powder was vibrated a diameter of 0.35mm standard screens, 121 DEG C of high pressures (1.035 × 10 by 6.5-7.55Pa it) sterilizes, 20-
30min twice, is dried for standby.
(2) LB culture mediums are prepared：Peptone 10g, yeast extract 5g, NaCl 10g, deionized water 1L, go out for 121 DEG C after mixing
Bacterium 20min；
(3) PGPR liquid bacterial agents are prepared：PGPR is respectively bacillus amyloliquefaciens S3-1 (Bacillus
Amyloliquefaciens S3-1), (preserving number：CCTTC AB2014337), Pseudomonas aurantica JD37 (Pseudomonas
AurantiacaJD37) (preserving number：CGMCC No.1.10967).Picking bacillus amyloliquefaciens S3-1 and orange false list respectively
Born of the same parents' bacterium JD37 single bacteriums are dropped down onto in 50ml LB liquid mediums, 28 DEG C, 200rpm culture 20h after, by 1% inoculation measure 10ml fermentation
Liquid is in 1000ml fresh liquid LB culture mediums, and shaken cultivation to logarithmic phase (28 DEG C, 200rpm, 20h), microbial inoculum bacteria containing amount is about
108CFU/ml。
(4) alkaline land soil biological modification agent is prepared：By PGPR liquid bacterial agents and matrix in mass ratio 1:1 stirring, it is natural
After air-drying, humic acid, then uniform stirring are added in, is fitted into plastic packaging bag and preserves.
The improved effect of the present invention is described in detail with reference to the embodiment：
It sets up inoculation alkaline land soil biological modification agent processing group and is not inoculated with control (CK), be inoculated with the salt-soda soil of this implementation
Soil conditioner processing group is alkaline land soil biological modification agent and alkaline land soil by volume 1:1 uniformly mixing.2 months
Interior, two processing groups spray equivalent sterile water, after 2 months, measure soil indices.
1st, influence of the alkaline land soil biological modification agent to soil physico-chemical index：
Influence of the alkaline land soil biological modification agent to soil physico-chemical index is as shown in table 1, after adding modifying agent, salt-soda soil
Soil physico-chemical index be improved significantly.PH decreased, EC values decline, and reduce murder by poisoning of the high salinity to vegetation.
Table 1
2nd, influence of the alkaline land soil biological modification agent to geobiont index
Add modifying agent after, alkaline land soil Biological indicators be improved significantly.Drop of the edaphon as organic matter
Solution person and the active library of plant nutrient substance, play an important role in soil nutrient, closely related with soil quality.Table 2
For influence of the alkaline land soil biological modification agent to soil microbe quantity, as shown in Table 2, using saline-alkali land soil conditioner
Afterwards, soil bacteria, fungi, Population of Actinomycetes increase, and alkaline land soil fertility are kept, raising substance recycles and it is more to improve biology
Sample has great importance.
Table 2
Soil enzyme activities is to weigh the important indicator of soil fertility, and table 3 is alkaline land soil biological modification agent to soil enzyme
The influence of activity, as shown in Table 3, soil urease, invertase, catalase activity are significantly improved after application modifying agent.
The ability of rhizosphere soil supply nitrogen can be improved by illustrating the application of modifying agent, to provide nitrogen source as growth；Enhance in soil
The conversion of organic carbon increases ease of solubility nutriment in soil；And soil hydrogen peroxide degradation can be promoted, it reduces soil and poisons.
Table 3
Embodiment 2
The present embodiment alkaline land soil biological modification agent is uniformly mixed to prepare by the component of following parts by weight content：
Wherein, the bacteria containing amount of PGPR liquid bacterial agents is 1 × 108CFU/ml。
In the present embodiment, contained PGPR is bacillus amyloliquefaciens S3-1 (Bacillus in PGPR liquid bacterial agents 1
AmyloliquefaciensS3-1), deposit number is CCTCC AB 2014337；The preservation time：On December 12nd, 2014；
Depositary institution：China typical culture collection center (China Center for Type CultureCollection), referred to as
CCTCC；Preservation address：(the first attached primary school pair of Wuhan University in the school of No. 299 Wuhan Universitys of Wuhan City, Hubei Province Wuchang District Bayi Road
Face), Wuhan University's collection.
Contained PGPR is Pseudomonas aurantica JD37 (Pseudomonas in PGPR liquid bacterial agents 2
AurantiacaJD37), deposit number is CGMCC No.1.10967；The preservation time：In May, 2011；Depositary institution：China
Microbiological Culture Collection administration committee common micro-organisms center (China General Microbiological Culture
Collection Center), abbreviation CGMCC；Preservation address：The Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences
Institute of microbiology.
The method that the present embodiment prepares the soil conditioner for being suitable for salt-soda soil is substantially the same manner as Example 1, is improved
The effect that has clear improvement to soil physico-chemical index, geobiont index and soil enzyme activities index of agent.
Embodiment 3
A kind of alkaline land soil biological modification agent of the present embodiment, is uniformly mixed to prepare by the component of following parts by weight content：
Wherein, the bacteria containing amount of PGPR liquid bacterial agents is 1 × 108CFU/ml。
In the present embodiment, contained PGPR is bacillus amyloliquefaciens S3-1 (Bacillus in PGPR liquid bacterial agents 1
AmyloliquefaciensS3-1), deposit number is CCTCC AB 2014337；The preservation time：On December 12nd, 2014；
Depositary institution：China typical culture collection center (China Center for Type CultureCollection), referred to as
CCTCC；Preservation address：(the first attached primary school pair of Wuhan University in the school of No. 299 Wuhan Universitys of Wuhan City, Hubei Province Wuchang District Bayi Road
Face), Wuhan University's collection.
Contained PGPR is Pseudomonas aurantica JD37 (Pseudomonas in PGPR liquid bacterial agents 2
AurantiacaJD37), deposit number is CGMCC No.1.10967；The preservation time：In May, 2011；Depositary institution：China
Microbiological Culture Collection administration committee common micro-organisms center (China General Microbiological Culture
Collection Center), abbreviation CGMCC；Preservation address：The Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences
Institute of microbiology.
The method that the present embodiment prepares the soil conditioner for being suitable for salt-soda soil is substantially the same manner as Example 1, is improved
The effect that has clear improvement to soil physico-chemical index, geobiont index and soil enzyme activities index of agent.
Embodiment 4
A kind of alkaline land soil biological modification agent of the present embodiment, is uniformly mixed to prepare by the component of following parts by weight content：
Wherein, the bacteria containing amount of PGPR liquid bacterial agents is 1 × 108CFU/ml。
In the present embodiment, contained PGPR is bacillus amyloliquefaciens S3-1 (Bacillus in PGPR liquid bacterial agents 1
AmyloliquefaciensS3-1), deposit number is CCTCC AB 2014337；The preservation time：On December 12nd, 2014；
Depositary institution：China typical culture collection center (China Center for Type CultureCollection), referred to as
CCTCC；Preservation address：(the first attached primary school pair of Wuhan University in the school of No. 299 Wuhan Universitys of Wuhan City, Hubei Province Wuchang District Bayi Road
Face), Wuhan University's collection.
Contained PGPR is Pseudomonas aurantica JD37 (Pseudomonas in PGPR liquid bacterial agents 2
AurantiacaJD37), deposit number is CGMCC No.1.10967；The preservation time：In May, 2011；Depositary institution：China
Microbiological Culture Collection administration committee common micro-organisms center (China General Microbiological Culture
Collection Center), abbreviation CGMCC；Preservation address：The Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences
Institute of microbiology.
The method that the present embodiment prepares the soil conditioner for being suitable for salt-soda soil is substantially the same manner as Example 1, is improved
The effect that has clear improvement to soil physico-chemical index, geobiont index and soil enzyme activities index of agent.It is above-mentioned to implementing
The description of example is for ease of ordinary skill in the art to understand and use the invention.Person skilled in the art
Obviously can easily these embodiments be made with various modifications, and General Principle described herein is applied to other embodiment
In without having to go through creative labor.Therefore, the present invention is not limited to above-described embodiments, and those skilled in the art are according to the present invention
Announcement, improvement and modification made without departing from the scope of the present invention all should be within protection scope of the present invention.
Claims (10)
1. a kind of alkaline land soil biological modification agent, which is characterized in that be uniformly mixed to prepare by the component of following parts by weight content：
The bacteria containing amount of the PGPR liquid bacterial agents 1 is (0.5-1.5) × 108CFU/ml；
The bacteria containing amount of the PGPR liquid bacterial agents 2 is (0.5-1.5) × 108CFU/ml。
2. a kind of alkaline land soil biological modification agent according to claim 1, which is characterized in that by following parts by weight content
Component be uniformly mixed to prepare：
The bacteria containing amount of the PGPR liquid bacterial agents 1 is 1 × 108CFU/ml；
The bacteria containing amount of the PGPR liquid bacterial agents 2 is 1 × 108CFU/ml。
A kind of 3. alkaline land soil biological modification agent according to claim 1 or 2, which is characterized in that the PGPR liquid
In body microbial inoculum 1 contained PGPR be bacillus amyloliquefaciens S3-1 (Bacillus amyloliquefaciensS3-1), preservation
Number is CCTCC AB 2014337；The preservation time：On December 12nd, 2014；Depositary institution：In China typical culture collection
The heart.
A kind of 4. alkaline land soil biological modification agent according to claim 1 or 2, which is characterized in that the PGPR liquid
Contained PGPR is Pseudomonas aurantica JD37 (Pseudomonas aurantiacaJD37) in body microbial inoculum 2, and deposit number is
CGMCC No.1.10967；The preservation time：In May, 2011；Depositary institution：China Committee for Culture Collection of Microorganisms is common
Microorganism center.
5. a kind of alkaline land soil biological modification agent according to claim 1 or 2, which is characterized in that the matrix is
Commercially available turf chooses the particle that grain size is 0.35-1.4mm, adjusts its pH value as 6.5-7.5.
A kind of 6. alkaline land soil biological modification agent according to claim 1 or 2, which is characterized in that the humic acid
In the mass content of chloride be less than or equal to 0.05%, the mass content of sulfate is less than or equal to 0.05%, and loss on drying is small
In equal to 2.0%, ignition residue is less than or equal to 0.5%.
7. a kind of preparation method of alkaline land soil biological modification agent as claimed in claim 1 or 2, which is characterized in that including
Following steps：
(1) matrix pre-processes：It is in mass ratio by calcium carbonate (0.5-2)：9 add in turfy soil, adjust its pH value as 6.5-7.5,
By turfy soil pulverize it is last vibrated a diameter of 0.35mm and 1.4mm standard screen choose grain size be 0.35-1.4mm
Grain, is dried for standby after sterilizing；
(2) PGPR liquid bacterial agents are prepared：By bacillus amyloliquefaciens S3-1 (Bacillus amyloliquefaciensS3-1)
It is inoculated in the fluid nutrient medium after sterilizing, training respectively with Pseudomonas aurantica JD37 (Pseudomonas aurantiacaJD37)
It supports and obtains PGPR liquid bacterial agents 1 and PGPR liquid bacterial agents 2；
(3) it mixes：PGPR liquid bacterial agents 1 and PGPR liquid bacterial agents 2 are mixed and stirred for uniformly, adding in after natural air drying in matrix
The humic acid of powdery is stirred for uniformly, obtaining alkaline land soil biological modification agent.
8. the preparation method of a kind of alkaline land soil biological modification agent according to claim 7, which is characterized in that described
Sterilize be 121 DEG C at a temperature of sterilize 20-30min, number is once or twice.
9. the preparation method of a kind of alkaline land soil biological modification agent according to claim 7, which is characterized in that described
Fluid nutrient medium is LB culture mediums, the LB liquid medium by added in every liter of deionized water the peptone of 10g, 5g yeast extracts and
It is stirred evenly after 10gNaCl obtained.
A kind of 10. preparation method of alkaline land soil biological modification agent according to claim 7, which is characterized in that step
(2) bacillus amyloliquefaciens S3-1 (Bacillus amyloliquefaciensS3-1) and the Pseudomonas aurantica JD37 in
The culture of (Pseudomonas aurantiacaJD37) is shaken cultivation 20 hours under conditions of 28 DEG C, 200r/min.
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