CN108138146A - 制备原代细胞样品的方法 - Google Patents

制备原代细胞样品的方法 Download PDF

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Publication number
CN108138146A
CN108138146A CN201680061008.6A CN201680061008A CN108138146A CN 108138146 A CN108138146 A CN 108138146A CN 201680061008 A CN201680061008 A CN 201680061008A CN 108138146 A CN108138146 A CN 108138146A
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cell
anoikis
inhibitor
sample
cells
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CN201680061008.6A
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Chinese (zh)
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L·G·莱恩
B·里驰
A·丹大帕特
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Celcuity Inc
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Celcuity Inc
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/0693—Tumour cells; Cancer cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C12N2501/734—Proteases (EC 3.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
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    • C12N2533/52—Fibronectin; Laminin
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
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  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN201680061008.6A 2015-10-20 2016-10-20 制备原代细胞样品的方法 Pending CN108138146A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562243765P 2015-10-20 2015-10-20
US62/243,765 2015-10-20
PCT/US2016/057923 WO2017070353A1 (en) 2015-10-20 2016-10-20 Methods of preparing a primary cell sample

Publications (1)

Publication Number Publication Date
CN108138146A true CN108138146A (zh) 2018-06-08

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Family Applications (1)

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CN201680061008.6A Pending CN108138146A (zh) 2015-10-20 2016-10-20 制备原代细胞样品的方法

Country Status (9)

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US (2) US11591573B2 (https=)
EP (1) EP3365436B1 (https=)
JP (2) JP7202613B2 (https=)
KR (1) KR102757122B1 (https=)
CN (1) CN108138146A (https=)
AU (1) AU2016341938B2 (https=)
CA (1) CA2999054A1 (https=)
DK (1) DK3365436T3 (https=)
WO (1) WO2017070353A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736A (zh) * 2019-01-29 2019-05-21 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN109943520A (zh) * 2019-03-08 2019-06-28 北京达博威迎医药技术有限公司 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
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AU2016341938B2 (en) * 2015-10-20 2022-12-01 Celcuity Inc. Methods of preparing a primary cell sample
CN108300688B (zh) * 2018-02-07 2021-02-02 上海赛立维生物科技有限公司 原代肝细胞分离和培养方法
EP4073516A1 (en) 2019-12-09 2022-10-19 Celcuity Inc. Methods of treating cancer patients with ras node or rtk targeted therapeutic agents
CN111733136B (zh) * 2020-06-29 2021-11-30 中山大学孙逸仙纪念医院 一种提高CD90posi细胞分离效率的方法
EP4425769A4 (en) 2021-10-27 2025-11-05 Sumitomo Bakelite Co STATOR AND STRUCTURE

Citations (3)

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EP1795897A1 (fr) * 2005-12-12 2007-06-13 Université de Limoges Test prédictif de réponse thérapeutique du cancer du sein
TW201439537A (zh) * 2012-10-25 2014-10-16 Academia Sinica 用於氧氣濃度控制下同步細胞培養的微流體陣列平台
WO2015089380A2 (en) * 2013-12-12 2015-06-18 Celcuity Llc Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent

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EP1795897A1 (fr) * 2005-12-12 2007-06-13 Université de Limoges Test prédictif de réponse thérapeutique du cancer du sein
TW201439537A (zh) * 2012-10-25 2014-10-16 Academia Sinica 用於氧氣濃度控制下同步細胞培養的微流體陣列平台
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736A (zh) * 2019-01-29 2019-05-21 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN109943520A (zh) * 2019-03-08 2019-06-28 北京达博威迎医药技术有限公司 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用
CN109943520B (zh) * 2019-03-08 2023-06-23 中国人民解放军军事科学院军事医学研究院 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用

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HK1252635A1 (zh) 2019-05-31
US11591573B2 (en) 2023-02-28
DK3365436T3 (da) 2021-10-11
US20180230434A1 (en) 2018-08-16
KR102757122B1 (ko) 2025-01-21
KR20180057653A (ko) 2018-05-30
JP2021175415A (ja) 2021-11-04
EP3365436B1 (en) 2021-09-08
WO2017070353A1 (en) 2017-04-27
EP3365436A1 (en) 2018-08-29
AU2016341938B2 (en) 2022-12-01
AU2016341938A1 (en) 2018-04-26
JP2018531021A (ja) 2018-10-25
CA2999054A1 (en) 2017-04-27
US12215356B2 (en) 2025-02-04
US20230235293A1 (en) 2023-07-27
JP7202613B2 (ja) 2023-01-12

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