CA2999054A1 - Methods of preparing a primary cell sample - Google Patents

Methods of preparing a primary cell sample Download PDF

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Publication number
CA2999054A1
CA2999054A1 CA2999054A CA2999054A CA2999054A1 CA 2999054 A1 CA2999054 A1 CA 2999054A1 CA 2999054 A CA2999054 A CA 2999054A CA 2999054 A CA2999054 A CA 2999054A CA 2999054 A1 CA2999054 A1 CA 2999054A1
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CA
Canada
Prior art keywords
sample
anoikis
cell
cells
inhibitor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA2999054A
Other languages
English (en)
French (fr)
Inventor
Lance Gavin Laing
Ben RICH
Abhijit Dandapat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Celcuity Inc
Original Assignee
Celcuity Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celcuity Inc filed Critical Celcuity Inc
Publication of CA2999054A1 publication Critical patent/CA2999054A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C12N2501/734—Proteases (EC 3.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
CA2999054A 2015-10-20 2016-10-20 Methods of preparing a primary cell sample Pending CA2999054A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562243765P 2015-10-20 2015-10-20
US62/243,765 2015-10-20
PCT/US2016/057923 WO2017070353A1 (en) 2015-10-20 2016-10-20 Methods of preparing a primary cell sample

Publications (1)

Publication Number Publication Date
CA2999054A1 true CA2999054A1 (en) 2017-04-27

Family

ID=57233884

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2999054A Pending CA2999054A1 (en) 2015-10-20 2016-10-20 Methods of preparing a primary cell sample

Country Status (9)

Country Link
US (2) US11591573B2 (https=)
EP (1) EP3365436B1 (https=)
JP (2) JP7202613B2 (https=)
KR (1) KR102757122B1 (https=)
CN (1) CN108138146A (https=)
AU (1) AU2016341938B2 (https=)
CA (1) CA2999054A1 (https=)
DK (1) DK3365436T3 (https=)
WO (1) WO2017070353A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102757122B1 (ko) 2015-10-20 2025-01-21 셀퀴티 인크. 1차 세포 샘플을 제조하는 방법
CN108300688B (zh) * 2018-02-07 2021-02-02 上海赛立维生物科技有限公司 原代肝细胞分离和培养方法
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736B (zh) * 2019-01-29 2022-03-25 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN115927162A (zh) * 2019-03-08 2023-04-07 中国人民解放军军事科学院军事医学研究院 汗腺细胞的培养获得汗腺类器官的方法及其应用
EP4073516A1 (en) 2019-12-09 2022-10-19 Celcuity Inc. Methods of treating cancer patients with ras node or rtk targeted therapeutic agents
CN111733136B (zh) * 2020-06-29 2021-11-30 中山大学孙逸仙纪念医院 一种提高CD90posi细胞分离效率的方法
WO2023074571A1 (ja) 2021-10-27 2023-05-04 住友ベークライト株式会社 ステータ及び構造体

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995018216A1 (en) 1993-12-30 1995-07-06 Nitta Gelatin Inc. Process for embedding culture of animal cells
US5858678A (en) 1994-08-02 1999-01-12 St. Louis University Apoptosis-regulating proteins
US8252591B2 (en) 2004-05-07 2012-08-28 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
ATE527341T1 (de) 2004-10-27 2011-10-15 Univ Bruxelles Hepatische differenzierung von stammzellen
FR2894594B1 (fr) * 2005-12-12 2008-02-08 Univ Limoges Test predictif de reponse therapeutique du cancer du sein
EA201070521A1 (ru) * 2007-11-20 2011-02-28 Опито Юниверситер Де Женева Способы идентификации, очистки и обогащения незрелых или стволовых клеток-инициаторов злокачественного роста из опухолей и их применение
WO2010026574A2 (en) 2008-09-02 2010-03-11 Pluristem Ltd. Adherent cells from placenta tissue and use thereof in therapy
US20130012404A1 (en) 2010-01-19 2013-01-10 Osaka Prefectural Hospital Organization Culture method, evaluation method and storage method for cancer-tissue-derived cell mass or aggregated cancer cell mass
EP2566975A4 (en) 2010-05-03 2014-04-02 Microstem Inc SCREENING METHODS, COMPOSITIONS IDENTIFIED BY THIS METHOD, TOOLS USEFUL FOR THEIR IDENTIFICATION AND CELLULAR POPULATIONS THUS PRODUCED
TWI477599B (zh) 2011-01-28 2015-03-21 鴻海精密工業股份有限公司 培育基體
SG11201401183UA (en) * 2011-10-04 2014-09-26 Mitra Biotech Private Ltd Ecm composition, tumor microenvironment platform and methods thereof
US20130330761A1 (en) 2012-06-12 2013-12-12 Celcuity, LLC Whole cell assays and methods
US20140142000A1 (en) * 2012-10-25 2014-05-22 Academia Sinica Microfluidic array platform for simultaneous cell culture under oxygen tensions
CA2895791A1 (en) * 2013-01-25 2014-07-31 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
GB201303491D0 (en) * 2013-02-27 2013-04-10 Cancer Rec Tech Ltd Culture of leukemia initiating cells
WO2015089380A2 (en) * 2013-12-12 2015-06-18 Celcuity Llc Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent
WO2015156929A1 (en) * 2014-04-07 2015-10-15 The Trustees Of Columbia University In The City Of New York Method for culture of human bladder cell lines and organoids and uses thereof
KR102757122B1 (ko) 2015-10-20 2025-01-21 셀퀴티 인크. 1차 세포 샘플을 제조하는 방법

Also Published As

Publication number Publication date
WO2017070353A1 (en) 2017-04-27
AU2016341938A1 (en) 2018-04-26
EP3365436B1 (en) 2021-09-08
JP2018531021A (ja) 2018-10-25
US20180230434A1 (en) 2018-08-16
JP7202613B2 (ja) 2023-01-12
US12215356B2 (en) 2025-02-04
US11591573B2 (en) 2023-02-28
EP3365436A1 (en) 2018-08-29
US20230235293A1 (en) 2023-07-27
DK3365436T3 (da) 2021-10-11
AU2016341938B2 (en) 2022-12-01
JP2021175415A (ja) 2021-11-04
KR20180057653A (ko) 2018-05-30
CN108138146A (zh) 2018-06-08
HK1252635A1 (zh) 2019-05-31
KR102757122B1 (ko) 2025-01-21

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