CN108125231A - A kind of health food containing ginseng and preparation method thereof - Google Patents
A kind of health food containing ginseng and preparation method thereof Download PDFInfo
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- CN108125231A CN108125231A CN201611089527.1A CN201611089527A CN108125231A CN 108125231 A CN108125231 A CN 108125231A CN 201611089527 A CN201611089527 A CN 201611089527A CN 108125231 A CN108125231 A CN 108125231A
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- ginseng
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of health foods containing ginseng and preparation method thereof.The health food is made of main components such as wilsonii, folium cortex eucommiae, ginsengs, by the preparation of rational compatibility and science, proves that this product has the healthcare function for alleviating physical fatigue through animal function test.
Description
Technical field
It is in particular to a kind of anti-containing ginseng the present invention relates to a kind of anti-fatigue health-product containing and preparation method thereof
Tired health products prescription and preparation method thereof.
Background technology
Fatigue is used as a kind of physiological phenomenon, refers to " the physiology course of body, it is impossible to which its function is maintained at a specified level
Or each organ cannot maintain its scheduled exercise intensity ".Fatigue is the comprehensive physiology for being related to many physiology factors
Process is a kind of physiological phenomenon necessarily occurred when human body is mental or physical exertion is to certain phase, it indicates that body is original
The temporary decline of ability to work, and may be the tendency that body develops to sick and wounded state.Human body feels fatigue, although unlike cancer
Disease, heart disease, the oncoming force is swift and violent, directly promptly causes death, but it is as a kind of stealthy killer for endangering modern's health,
The work and life of people have been seriously affected, has considerably increased sub-health population quantity.
Continuous with social progress, rhythm of life and working strength accelerates and increases, " fatigue " and " overwork " increasingly
It is taken seriously.If cannot timely alleviate after fatigue generation, it will gradually accumulation eventually leads to overwork, occurs chronic tired
Labor syndrome.Burnout syndrome may occur in which：Be not easy to eliminate it is tired out, be weary of, irritated, absent minded, unknown cause the heart
Flurried meaning disorderly, dizzy, headache, constipation or diarrhea, skin occur color spot, apocleisis, abdominal distension, sexuality decline, hypertension, hyperlipidemia,
Fatty liver etc..Having 14% adult male and 29% women in the U.S. has chronic fatigue syndrome；In Japanese working population
There is more than 30% to be attacked by this disease, wherein having 40% people's cisco unity malfunction and study.Also there are more and more crowds in China
The puzzlement of this disease is subjected to, is such as engaged in scientific research, IT industries personage and reporter, performers, marketer, country's public affair on the middle and senior level
Member, enterprise manager on the middle and senior level etc..
Following a few classes can be substantially divided by alleviating the product of physical fatigue currently on the market：First is supplement energy；Second
It is supplement vitamin and trace element needed by human；Third is the function by improving biological organs, the particularly circulatory system
Function, the removing of acceleration bodies intracellular metabolite substance excludes, to achieve the purpose that alleviate physical fatigue.It is inquired according to SFDA official websites, mesh
Physical fatigue health food 951, wherein national OTC 927 are alleviated in preceding state food pharmaceuticals administration general bureau approval
It is a, import health food 24.Such health products often have with raw material：Ginseng, American Ginseng, wilsonii, Radix Angelicae Sinensis, Radix Astragali, rhodiola root,
The Chinese medicines such as matrimony vine, pueraria lobata, ganoderma lucidum and octacosanol, taurine, fish gill glue, wheat germ oil, caffeine, oyster extract etc..
Invention content
Health products of the present invention are using ginseng, wilsonii, folium cortex eucommiae as raw material, and ginseng reinforces vital energy, veins takes off admittedly, tonifying spleen benefit
Lung；Wilsonii replenishes qi to invigorate the spleen, and tonifies the kidney to relieve mental strain；Folium cortex eucommiae filling liver kidney, strengthening the bones and muscles.Three taste raw materials coordinate jointly so that human body liver and spleen
The dirty function that must be mended, improve biological organs of kidney three, so as to reach the effect of alleviating physical fatigue.
Raw material ginseng, wilsonii, folium cortex eucommiae selected by this product record " available in " defending method prison hair ［ 2002 ］ 51 " attachment 2
In the article list of health food " in, materials safety.And this product is based on TCM theory, to alleviate physical fatigue
Aspect plays due health-care efficacy.Healthcare function is clear and definite, no incompatibility.
In screening this product formula, we are examined in terms of raw material legitimacy, healthcare function, feasibility etc. are several
It examines, finally filters out this formula.
Ginseng is exactly health treasure since ancient times, has thousands of years of medicinal and health care history.Wilsonii is from ancient times to the present
It is considered as, with the good medicine for adding smart mends marrow and anti-aging effects, to replenish qi to invigorate the spleen, tonifying the kidney to relieve mental strain.Folium cortex eucommiae has filling liver kidney, strong
The effect of muscles and bones.
Ginseng reinforces vital energy in formula, veins takes off admittedly, reinforces the spleen to benefit the lung as monarch；Wilsonii replenishes qi to invigorate the spleen, and tonifies the kidney to relieve mental strain；Cortex Eucommiae
Leaf filling liver kidney, strengthening the bones and muscles are altogether minister.To sum up, above-mentioned raw materials are used cooperatively, and full side's invigorating the spleen tonifying Qi is tonified the kidney to relieve mental strain, nourishing liver and replenishing blood,
It must be mended so that human body liver and spleen kidney three is dirty, physical fatigue is alleviated in common cooperation.
Health food of the present invention containing ginseng, it is characterised in that the parts by weight of each major ingredient of beverage are as follows：
800-1200 parts of wilsonii, 500-700 parts of folium cortex eucommiae, 100-300 parts of ginseng.
Preferably, the parts by weight of each major ingredient of health food of the present invention are as follows：800 parts of wilsonii, 700 parts of folium cortex eucommiae, ginseng
100 parts.
Preferably, the parts by weight of health food major ingredient of the present invention are alternatively：1200 parts of wilsonii, 500 parts of folium cortex eucommiae, ginseng
300 parts.
Preferably, the parts by weight of health food major ingredient of the present invention are alternatively：1000 parts of wilsonii, 667 parts of folium cortex eucommiae, ginseng
217 parts.
Preferably, the parts by weight of health food major ingredient of the present invention are alternatively：1080 parts of wilsonii, 658 parts of folium cortex eucommiae, ginseng
228 parts.
Health food of the present invention further includes the required auxiliary material of preparation, and auxiliary material is starch, and starch used is jade
Rice starch or starch from sweet potato.50-70 parts of number shared by auxiliary material, it is preferred that starch can be 50 parts, 59 parts, 66 parts or 70 parts.
The preparation formulation of health food of the present invention can be capsule, tablet, pill, powder, granule or cream
Agent.
Health food of the present invention, the preparation method of capsule include the following steps：
A, pretreatment of raw material：Ginseng crushes, sieving, and ginseng comminuted powder is spare；
B, it extracts：Wilsonii and folium cortex eucommiae are weighed by formula ratio, the 8-12 times of water measured is added in and extracts 1-2 times, extracting solution filtering, often
It is 1-2 hours secondary, merge Aqueous extracts, it is spare；
C, it concentrates：Aqueous extracts obtained by step b are concentrated, concentrate is spare；
D, drying：Concentrate obtained by step c is dried, dry cream is spare；
E, it crushes：Dry cream crushing obtained by step d is sieved for subsequent use；
F, it mixes：Ginseng comminuted powder obtained by step a is sterilized, adds in comminuted powder and starch obtained by step e, is uniformly mixed,
It is spare to obtain mixed powder；
G, it pelletizes：Mixed powder obtained by step e is pelletized, particle is spare；
H, by obtained by step g grain packing capsule, packaging, examine to obtain the final product.
The preparation method of preferred capsule includes the following steps：
A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, irradiation, it is spare that ginseng crushes sterilized powder；
B, it extracts：Wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 12 times of amounts, second of 10 times of amount, often
Secondary extraction 1 hour, filtering, Aqueous extracts are spare；
C, it concentrates：By the Aqueous extracts obtained by step b in temperature 60-80 degree, vacuum degree is concentrated under the conditions of being 0.04-0.10Mpa
It is spare that 60 degree of heat survey the concentrate that relative density is 1.10-1.20；
D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 60-80 degree, and vacuum degree 0.04-0.10Mpa is obtained
It is spare to dry cream；
E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；
F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and starch obtained by step e, is uniformly mixed,
It is spare to obtain mixed powder；
G, it pelletizes：By the mixed powder dry granulation obtained by step e, it is spare to obtain particle；
H, by obtained by step g grain packing capsule, packaging, examine to obtain the final product.
Preferably, the preparation method of granule is as follows：
A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, irradiation, it is spare that ginseng crushes sterilized powder；
B, it extracts：Wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 12 times of amounts, second of 10 times of amount, often
Secondary extraction 1 hour, filtering, Aqueous extracts are spare；
C, it concentrates：By the Aqueous extracts obtained by step b in temperature 60-80 degree, vacuum degree is concentrated under the conditions of being 0.04-0.10Mpa
It is spare that 60 degree of heat survey the concentrate that relative density is 1.10-1.20；
D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 60-80 degree, and vacuum degree 0.04-0.10Mpa is obtained
It is spare to dry cream；
E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；
F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and starch obtained by step e, is uniformly mixed,
It is spare to obtain mixed powder；
G, it pelletizes：By the mixed powder dry granulation obtained by step e, whole grain is packed, is examined to obtain the final product.
This product technique is using conventional capsule agent producing process：1. ginseng → crushing → sterilizing；2. wilsonii, folium cortex eucommiae
→ water carries, filtering → Aqueous extracts concentration, drying, crushes；3. ginseng pulverate, dried cream powder, cornstarch → mixing → granulation → whole grain
→ total mixed → filling → polishes → selecting → and packs → examine storage.
Technological parameter determines
1st, ginseng crushes
Take clean, three parts of dried ginseng, every part weight 1000g, crush, cross 80 mesh sieve（Because ginseng is valuable medicinal, dress is crushed
Capsule was largely 80 mesh sieve）, fine powder is collected, is weighed, calculates flour extraction.As seen from the experiment, ginseng is parallel crushes 3
Secondary, for flour extraction more than 97%, average flour extraction is 97.3%.
2nd, ginseng pulverate's sterilization process is studied
Because ginseng directly to beat powder, need to sterilize to ginseng pulverate in this product.This product is sterilized using 60Co irradiation.It investigates not
With influence of the irradiation dose to ginseng pulverate's microbial limit and total saponin content, the results show that 60Co irradiation doses are in 0 ~ 5kGy
In the range of, the contents of total saposins is without significant change, but the quantity of bacterium, mould and saccharomycete, with the increase of irradiation dose
Gradual to reduce, when irradiation dose reaches 4kGy, microorganism indices meet the requirements, accordingly, it is determined that mixed powder 60Co spokes
It is 4kGy according to dosage.
3rd, it extracts
（1）The investigation of water absorption rate
Wilsonii, folium cortex eucommiae medicine materical crude slice are weighed by formula rate, adds 10 times of amount water, is dipped to and is impregnated with completely, soak filtering measures
Volume calculates water absorption rate.Experimental result shows, wilsonii, folium cortex eucommiae medicine materical crude slice water absorption rate are 1.91ml/g, about 2 times of medicinal material weights
The water absorption of amount, is prompted in subsequent extracted, should add 2 times of solvents for the first time.
（2）Orthogonal Experiment and Design
Wilsonii, folium cortex eucommiae medicine materical crude slice, add water to cook in formula, its extraction process is taken just in extracting solution filtering, concentration, drying
Design method is handed over to be investigated using total saponin content in extracting solution as index.
Using the content of total saposins as inspection target, intuitively analysis shows, the influence sequence of three factors is A（Extraction time）
＞ B（Extraction time）＞ C（Solvent dosage）, the optimum process condition intuitively analyzed is that 10 times are measured water refluxing extraction 3 times, every time
2 hours.Variance analysis shows that B, C factor do not have a significant impact, and A factors have a significant impact.In B factors, with extraction
The increase of time, the content of total saposins change without conspicuousness；The extraction solvents of different times of amounts in C factors, the contents of total saposins without
Conspicuousness changes；In A factors, total saponin content differs greatly in extracting solution when extraction time is for 1 time and 2 times, and extracts secondary
The difference of total saponin content has been obviously reduced in extracting solution when number is 2 times and 3 times.It is thus determined that preferred processing condition.
Consider later stage actual production and production cost factor, extraction time selects 2 times, and extraction time is chosen 1 hour
It is more suitable.It is thus determined that wilsonii, folium cortex eucommiae extraction process are heating and refluxing extraction 2 times, 1 hour every time, add 12 for the first time
Amount water again, second plus 10 times of amount water.
4 concentration technology parameters determine
It is extracted by determining extraction process, extracting solution is concentrated under reduced pressure at 60 DEG C, 70 DEG C, 80 DEG C respectively, is concentrated into
Relative density is 1.10 ~ 1.20（60℃）When take out, measure total saponin content and simultaneously calculate the rate of transform.By result it is found that concentration temperature
For degree in the range of 60 ~ 80 DEG C, the total saposins rate of transform is very high in concentrate, therefore thickening temperature is chosen to be 60 ~ 80 DEG C.
Concentration technology parameter is as follows：Condensing mode：It is concentrated under reduced pressure；Thickening temperature：60~80℃；Vacuum degree：0.04~0.08
MPa；Concentrate relative density：1.10~1.20（60 DEG C of surveys）.
5 Drying Technology Parameters determine
Concentrate is taken to be dried in vacuo at 60 DEG C, 70 DEG C, 80 DEG C, measure total saponin content in dried cream powder and calculates transfer
Rate.By result it is found that thickening temperature is in the range of 60 ~ 80 DEG C, the total saposins rate of transform is very high in dried cream powder, it is thus determined that dry
Temperature is 60 ~ 80 DEG C.
Drying parameter determines as follows：Drying mode：Vacuum drying；Drying temperature：60~80；Vacuum degree：0.04~0.10
MPa。
6 dry creams crush
The dry cream prepared is put in pulverizer and is crushed.Sieve mesh number：80 mesh.
7 granulating process are studied
Common granulation mode has wet granulation and dry granulation, and wet granulation process is more complicated, and material needs after adding in binder
It dries again, the production cycle is long.Dry granulation pelletization need not then add binder, mixing softwood be omitted, drying waited
Journey shortens process route and production cycle, has saved cost.Therefore this product uses dry granulation technology.
To improve granulating efficiency, consider to add in cornstarch as starch.5 parts of mixed powder is taken, is separately added into cornstarch
It is 10%, 13%, 15%, 17%, 20% to measure, and after mixing, is pelletized respectively under 0.0 ~ 0.1mm pinch roller gap parameters, according to
Granulating efficiency determines final cornstarch addition, by experimental result it is found that when cornstarch addition is to 17%, mixes
It is best to close powder granulating efficiency.
8 incorporation times are investigated
Ginseng pulverate, dried cream powder, cornstarch are taken, is put in mixing machine, it is separately sampled when incorporation time 15,25,35 minutes, each
Time point is in parallel 5 parts of the sampling of mixing machine different location, total saponin content in determination sample, and calculate RSD values respectively, according to survey
Result is determined to determine best incorporation time, by experimental result it is found that three different time points samplings, each period are parallel
Total saponin content difference is little in 5 parts of samples, considers production cost factor, and it is 15 minutes to determine incorporation time.
The investigation of 9 pinch roller spacing technological parameters
The mixed powder prepared is taken, parallel three parts, investigates dry granulation pinch roller spacing technological parameter, as a result
The result shows that pinch roller spacing control granulating efficiency in 0.0 ~ 0.1mm is good.By pinch roller spacing in the range of 0.0 ~ 0.1mm
Obtained particle crosses the taper sieve whole grain of 1.5mm, and heap density and the angle of repose of particle, dry method system are measured with fixed funnel method
Grain gained particle, heap density are 0.6751, and angle of repose is 37.11 °, meets the demand of production mobility, can direct capsule charge.
Dry granulation process parameter determines as follows：Cornstarch addition：17%；Material incorporation time：15min；Between pinch roller
Away from：0.0 ~ 0.1mm, whole grain sieve mesh number：1.5mm.
The science of 10 product form and dosage form selection, reasonability, feasibility and the selection according to product form and dosage form
Mainly depending on the property of raw material, efficacy effect, the length for taking the phase and the situation of user.The dosage form selection of this product is
Capsule, reason are as follows：
（1）This product is raw materials used by crushing, and extracts, concentration is dry, mixing, after the series of process such as granulation, is flowed
Property, particle that heap density is suitable for is suitble to using industrialization automatic equipment filling capsule.
（2）Capsule is one of widest dosage form of current clinical practice, and preparation process is simple, appearance is clean and tidy, beautiful,
Have the advantages that dosage it is accurate, using convenience, stable quality, production mechanization, high degree of automation.
（3）Capsule is clean and tidy, beautiful, can cover the bad smell of drug.The raw material that this product has directly beats powder, some originals
Material is extracted with water, concentrated, dried, and therefore, content has the distinctive smell of this product, and powder color is less beautiful, dresses up glue
After capsule, raw material some smells in itself can be not only covered, integrally seems clean and tidy, beautiful, improves and take compliance.
Health food of the present invention is safe and non-toxic, and with antifatigue health-care effect, in order to verify health food of the present invention
Said effect, inventor will send Fujian Disease Control and Prevention Center to test by finished product made from embodiment 4, inspection result and sentence
Determine opinion:
Inspection result and judgement opinion:
1st, acute toxicity test:The sample the acute toxicity tests are LD50>20g/kg BW (are equivalent to Coming-of-Age Day recommendation
444 times of amount), nontoxic grade is belonged to according to acute toxicity classification.
2nd, Salmonella reversion test:The sample Salmonella reversion test result is feminine gender.
3rd, Micronucleus test:The sample mouse marrow cell micro nuclear test result is feminine gender.
4th, mouse inbred strain:The sample mouse Sperm Abnomality result of the test is feminine gender.
Survey report：Acute toxicity test
First, material:
1st, sample:Health food content of the present invention, Coming-of-Age Day recommended amounts are 2.7g, are equivalent to 0.045g/kg BW (adults
Weight is in terms of 60kg).
2nd, animal:The cleaning grade health ICR mouse that Shanghai Slac Experimental Animal Co., Ltd. is selected to provide, perhaps
It can the number of card:SCXK (Shanghai) 2012-0002.
3rd, feed:Bio tech ltd, credit number are risen for native Hai Pulu:Raise card (2014) 04001 in Shanghai.
4th, feeding environment:Fujian Center for Disease Control ＆ Prevention SPF grades of Animal Lab., credit number SYXK (Fujian)
2012-0008。
5th, detecting instrument:Electronic balance.
2nd, experimental method:
Choose 18-22g health ICR mouse 20, half male and half female.Mouse fasting overnight 16 hours, can't help water before experiment.Weigh this
Invention health food content 20g adds distilled water to be stirred evenly to 40mL, and interval 4h gives 2 times in a manner of oral gavage, gavage
Capacity is 0.02mL/g BW, and test dose is 20g/kg BW.After gavage, it is observed continuously 14 days, observes and records animal poisoning
Performance and death condition.
3rd, result:
Animal no abnormality seen during experiment;Surviving animals carry out gross anatomy, animal no abnormality seen after observation period.As a result see
Table 1.
4th, conclusion
The sample the acute toxicity tests are LD50>20g/kg BW (444 times that are equivalent to Coming-of-Age Day recommended amounts), according to
Acute toxicity classification belongs to nontoxic grade.
Inspection project：Salmonella reversion test
First, material：
1st, sample：Health food content of the present invention.
2nd, positive substance 2,4,7-TNFone, NaN3, MMC, 2-AF, 1,8- dihydroxy anthraquinones.
3rd, solvent:Distilled water, 0.103MPa20min sterilizings.
4th, detecting instrument:BSC-1360IIA2 Biohazard Safety Equipments, SHP-250 biochemical cultivation cases, DK-S28 electric heating constant temperatures
Water temperature case, electronic balance, SS-325 type automatic high pressures sterilizer, Automatic Colony Counter.
2nd, experimental method:
Using identified satisfactory tetra- plants of bacterium of Salmonella typhimurium histidine deficient TA97, TA98, TA100 and TA102
Strain is tested.Using the rat liver homogenate of Polychlorinated biphenyls induction as Metabolic Activation of Cyclophosphamide.Weigh health care food of the present invention
Product content 5.0g solubilizers, as high dose tested material, it is equal to 100 mL stirrings to measure high dose 20mL solubilizers to 100mL
It is even as secondary high dose tested material, three dosage and so on preparation, is made into 5000,1000,200,40 from high to low below
, 5 concentration of 8ug/ wares, sterilize through 0.055MPa20min, while set from beaming back change group, solvent control group and positive controls,
Each dosage sets 3 plates.Added in top agar 0.1 mL test strains enrichment liquid, 0.1mL tested materials solution and
0.5mLS-9 mixed liquors (when needing metabolism activation) are poured on bottom culture medium flat plate after mixing, small in 37 DEG C of cultures 48
When, it counts and change clump count is returned per ware, experiment is repeated once.If the change clump count that returns of sample is that solvent control group returns change clump count
2 times or more, and then it is set to the result of the test positive with dose-response relationship.
As a result:
By table 2,3 as it can be seen that returning for each dosage group of the sample becomes 2 times that clump count is less than solvent control group time change clump count.
4th, conclusion:
The Ames result of the tests of the sample are feminine gender.
Inspection project:Micronucleus test
First, material:
1st, sample:Health food content of the present invention, Coming-of-Age Day recommended amounts are 2.7g, are equivalent to 0.045g/kg BW (adults
Weight is in terms of 60kg).
2nd, animal:Purchased from the cleaning grade health ICR mouse that Shanghai Slac Experimental Animal Co., Ltd. produces, license
Card number:SCXK (Shanghai) 2012-0002.
3rd, feed:Shanghai Pu Lu rises bio tech ltd, credit number:Raise card (2014) 04001 in Shanghai.
4th, feeding environment:Fujian Center for Disease Control ＆ Prevention SPF grades of Animal Lab., credit number:SYXK (Fujian)
2012-0008。
5th, positive control:Cyclophosphamide (40mg/kgBW).
6th, solvent control object:Distilled water.
7th, detecting instrument:OlympusCH microscopes, electronic balance.
2nd, experimental method:
Using interval 24 hours, oral gavage was tested twice, chose weight 25-30g healthy mices 50, half male and half female, respectively
Gender mouse is randomly divided into 5 groups by weight, every group 5, and experiment sets 3 dosage groups as 2.5, and 5.0,10.Og/kg BW are equivalent to
55,111,222 times of recommended amounts of being grown up;Separately set solvent control group, positive controls.Health food content of the present invention is weighed respectively
Object 2.5,5.0,10.Og respectively add distilled water to be made into 3 concentration to 20mL, and each group animal is filled for 24 hours by 0.02mL/gBW intervals respectively
Stomach gives the tested material of various concentration and reference material 2 times, and cervical dislocation puts to death animal after last gavage 6 hours, and bone marrow of sternum is taken to use
Calf serum dilutes smear, is fixed through methanol, Giemsa dyeing.It is shown in optics and does Microscopic observation, every animal counts 1000
Polychromatic erythrocyte, microkernel incidence is to contain in terms of micronucleus PCE permillages, by x2It examines for statistical analysis.200 are counted simultaneously
PCE/NCE values are obtained in PCE.
3rd, result:
By table 4 as it can be seen that each dosage group PCE/NCE of the sample is not less than the 20% of solvent control group, erythrocyte system is prompted
Proliferation have no apparent inhibition, the observation of polychromatic erythrocyte micronucleus is had no significant effect.Positive controls microkernel incidence is bright
It is aobvious to be higher than solvent control group, the statistically significant (P of difference<), O.O1 each dosage group of sample and solvent control group comparing difference
Not statistically significant (p>0.05), illustrate that the sample does not show the mutagenic effect for mouse bone marrow cells PCE micronuclear rates rising of sening as an envoy to.
4th, conclusion：
The sample mouse marrow cell micro nuclear test result is feminine gender.
Inspection project:Mouse inbred strain
First, material:
1st, sample:Health products content of the present invention, Coming-of-Age Day recommended amounts are 2.7g, are equivalent to 0.045g/kg BW (adult body
Weight is in terms of 60kg).
2nd, animal:Cleaning grade health ICR mouse purchased from Shanghai Slac Experimental Animal Co., Ltd.'s production, perhaps
It can the number of card:SCXK (Shanghai) 2012-0002.
3rd, feed:Shanghai Pu Lu rises bio tech ltd, credit number:Raise card (2014) 04001 in Shanghai.
4th, feeding environment, S PF grades of Animal Lab. of Fujian Center for Disease Control ＆ Prevention, credit number: SYXK
(Fujian) 2012-0008.
5th, positive control：Cyclophosphamide (40 mg/kg BW).
6th, solvent control object:Distilled water.
7th, detecting instrument:Olympus CH microscopes, electronic balance.
2nd, experimental method:
Weight 25-30g sexal maturity male mice 25 is selected, is randomly divided into 5 groups by weight, every group 5, experiment sets 3 dosage
Group is 2.5,5.0,10.0g/kg BW, be equivalent to 55,111,222 times of adult's recommended amounts, separately set solvent control group, the positive
Control group.Weigh respectively health food content 2.5 of the present invention, 5.0,10.0g respectively add distilled water to 20mL be made into 3 it is dense
Degree, each group animal are given the tested material and reference material of various concentration by 0.02 mL/g BW gavages respectively, continuous 5 days.In for the first time
35 days after gavage, animal is put to death, takes bilateral epididymal tail by standardization program film-making, dye, every animal observation 1000 under microscope
A complete sperm records all kinds of lopsided chair numbers, sperm distortion incidence is calculated, by x2It examines for statistical analysis.
3rd, result:
By table 5 as it can be seen that each dosage group of the sample is compared with solvent control group, no significant difference (P>0. 05), positive
Control group is compared with solvent control group, the statistically significant (P of difference<0.01).Sample does not produce Sperm Abnormalities of Mice
Life is substantially change, and the sample is prompted not generate distortion effect to mouse sperm.
4th, conclusion：The sample mouse inbred strain result is feminine gender.
Inspection project:30 days feeding trials
Each dosage group of health products of the present invention is given rat and is fed 30 days, and during experiment, the activity of each group growth of animal is normal, each dosage
Weight, weight gain, total food-intake, total food utilize at the end of each all weight of group rat, food-intake, food utilization and experiment
Rate, dirty body ratio compared with the control group, the not statistically significant (P of difference>0.05).Male high dose group red blood cell count(RBC);Female
Low dose group glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease, blood glucose, T-CHOL compared with the control group, the statistically significant (P of difference<
0.05), but in this laboratory range of normal value, abiology meaning is thought in this laboratory.Remaining each dosage group rat
Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease, urea, flesh are intoxicated, blood glucose, T-CHOL, triglyceride, total protein, albumin measuring
Value, hemoglobin, red blood cell count(RBC), white blood cell count(WBC) and leukocyte differential count compared with the control group difference it is not statistically significant (
P> 0.05 ).To being made histopathological examination by inspection internal organs, specific lesions are had no.
1 material and method
1.1 sample:Health food content of the present invention, consigner provide capsule 's content for examination.Coming-of-Age Day recommended amounts are
2.7g is equivalent to 0.045g/kg BW (adult's weight is in terms of 60kg).Weighing sample 90g before use adds distilled water to stir to 200mL
It mixes uniformly, as high dose group tested material;Measure high dose group tested material by 1/2 multiple proportions successively decrease dilution be made into, low dose group
Tested material stirs evenly, for examination.
1.2 experimental animal:The cleaning grade SD rats 80 that Shanghai Slac Experimental Animal Co., Ltd. is selected to provide
Only, half male and half female, weight 72-83g.Credit number:SCXK (Shanghai) 2012-0002.
1.3 feeding environment:Fujian Center for Disease Control ＆ Prevention SPF grades of Animal Lab., credit number:SYXK (Fujian)
2012-0008 。
1.4 feed:Feed rises bio tech ltd, credit number purchased from Shanghai Pu Lu:Raise card (2014) in Shanghai
04001。
1.5 instrument:Hitachi's 7060C automatic clinical chemistry analyzers, SHANDON EXCELSIOR biological cells take off automatically
Water dispenser, BM-Vl biological tissues frozen embedding machine, SHANDON Finesse325 paraffin sections machine, CS-IV booth piece bake piece machine,
Electric drying oven with forced convection, OLYMPUS BX-51 microscopes, CELLDUN3700 Abbott Laboratories blood cell analysis machine, full-automatic dyeing machine, electricity
Sub- balance.
1.6 test method:Each gender animal is randomly divided into 4 groups, every group each 10 by weight.Experiment is by the tested material day for human beings
25,50 and 100 times of recommended amounts, if 3 dosage groups are 1. 12,2.2 5,4.50g/kg BW, separately set distilled water to be empty
White control group.It because of sample bitter, is given in a manner of oral gavage, gives various concentration by the oral gavage of 10mL/kg weight daily
Tested material and reference material are primary.Animal single cage is fed during experiment, ad lib drinking-water, observes zoomorphism daily, and record is raised
Material spills leakage quantity, records a weight and 2 food intake doses weekly.Animal is weighed after feeding 30 days overnight fastings, and blood is made in blood sampling
Routine and Biochemical Indexes take out liver, kidney, board, testis and weigh, and make pathology to liver,kidney,spleen, stomach and intestine, guilt nine, ovary
It checks.
1.7 observation index：
1.7.1 general performance, weight, food-intake, the food utilization of animal.
1.7.2 blood routine and biochemical indicator:Red blood cell count(RBC), white blood cell count(WBC), leukocyte differential count, hemoglobin, paddy third
Transaminase, glutamic-oxalacetic transaminease, urea, flesh are intoxicated, T-CHOL, triglyceride, blood glucose, total protein, albumin.
1.7.3 pathological anatomy:Dirty body ratio, gross examination of skeletal muscle and histopathologic examination (liver,spleen,kidney, stomach and intestine, guilt ball and ovum
Nest).
1.8 experimental data statistical methods:Experimental data carries out one-way analysis of variance with SPSS softwares.Through homogeneity of variance
Examine, the neat experimental data of variance is for statistical analysis using LSD methods, the experimental data of heterogeneity of variance using Tambane methods into
Row statistical analysis.
2 results
2.1 gross morphologies are observed:
The rat hair quality of 3 dosage groups of health food content of the present invention is white, glossy, and mode of appearance is compared with the control group without area
Not, without the phenomena of mortality yet.
2.2 animals not increase again and food utilization:
By table 6,7,8 as it can be seen that during the experiment, at the end of each all weight of each dosage group rat, food-intake, food utilization and experiment
Weight, weight gain, total food-intake, total food utilization rate compared with the control group, the not statistically significant (P of difference>0.05) .
2.3 blood routine examination results
By table 9 as it can be seen that male high dose group red blood cell count(RBC) cell compared with the control group, the statistically significant (P of difference<
0.05), but in this laboratory range of normal value, abiology meaning is thought in this laboratory.The blood red egg of remaining each dosage group
In vain, red blood cell count(RBC), white blood cell count(WBC) and leukocyte differential count compared with the control group, the not statistically significant (P of difference> 0.05 ).
2.4 serum biochemistry inspection results
By table 10 as it can be seen that female low dose group glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease, blood glucose, T-CHOL compared with the control group, it is poor
Different statistically significant (P<0.05), but in this laboratory range of normal value, this laboratory thinks that abiology is anticipated
Justice.Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease, urea, creatinine, T-CHOL, triglycerides, the blood of remaining each dosage group rat
Sugar, total protein, albumin compared with the control group, the not statistically significant (P of difference> 0 .05 ) .
2. 5 pairs of dirty bodies of rat than influence:
By table 11 as it can be seen that after each dosage group Rat Fast weight, dirty body ratio compared with the control group, difference it is not statistically significant (
P> O. 05 )。
2.6 histopathologic examination
2.6.1 each dosage group animal gross examination does not find apparent lesion, and biochemical indicator no abnormality seen, therefore only choosing control
Group and high dose group make histopathological examination.
2.6.2 result describes:
Liver:Normal lobuli hepatis structure exists, and without obvious tumefaction and granular degeneration, indivedual liver cell endochylemas are interior a little liver cell to occur
Tiny fat vacuole, becomes in mild fatty, and this fat becomes liver cell in special mess distribution is dispersed in, and range is small.Liver cell is without necrosis
Change.The cell infiltrations such as lymphocyte, no proliferation of fibrous tissue, bile duct no abnormality seen are seen in indivedual liver portal areas.
Stomach and duodenum:Mucous membrane is complete, and no apparent bleeding, necrosis waste that rotten, y is expensive to die young and cell infiltration, without apparent gland
The atrophy of body hyperplasia changes.Kidney:Kidney skin medullary substance structure understands, indivedual renal tubule mild swellings and vacuolar degeneration, in indivedual renal interstitials
There is a stove cell infiltration, visible cast in indivedual renal tubules.
Spleen:Splenic white pulp, red pulp structure understand that white pulp, red pulp are without apparent expansion or atrophy phenomenon.
Testis:Convoluted seminiferous tubule is normal, it is seen that spermatogenic cells at different stages and mature sperm, indivedual interstitial tissue[of testis] Mild edemas.Ovum
Nest:Development is normal, it is seen that Follicles and mature corpus luteum.
In short, Yi Shang pathological change only occurs in individual animal, each group specimens pathological variation no significant difference, therefore
Think that tested internal organs have no the pathological change related with tested material (variation of each group specimens pathological refers to table 12).
3 brief summaries
Each dosage group of health food of the present invention is given rat and is fed 30 days, and during experiment, the activity of each group growth of animal is normal, each dose
Weight, weight gain, total food-intake, total food profit at the end of each all weight of amount group rat, food-intake, food utilization and experiment
With rate, dirty body ratio compared with the control group, the not statistically significant (P of difference>0.05).Male high dose group red blood cell count(RBC);It is female
Property low dose group glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease, blood glucose, T-CHOL compared with the control group, the statistically significant (P of difference
<0.05), but in this laboratory range of normal value, abiology meaning is thought in this laboratory.Remaining each dosage group rat
Serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease, urea, creatinine, blood glucose, T-CHOL, triglyceride, total protein, albumin measuring
Value, hemoglobin, red blood cell count(RBC), white blood cell count(WBC) and leukocyte differential count compared with the control group difference it is not statistically significant (
P> 0. 05 ) .To being made histopathological examination by inspection internal organs, specific lesions are had no.
Inspection project：Function of physical fatigue alleviation is tested：
As a result：As a result the health food of the present invention that oral gavage gives mouse various dose can extend mouse swimming with a load attached to the body after 30 days
It swims the time;Reduce the serum urea content generated after mouse movement;Mouse blood lactase acid area under the curve is reduced, and to mouse weight
Increase without influence.Illustrate that the sample has and alleviate physical fatigue effect.
Sample ID:Health food of the present invention examines project:Function of physical fatigue alleviation is tested
1. material and method:
1.1 sample:Health food content of the present invention weighs sample 5.4g and distilled water is added to be stirred evenly to 80mL, matches every time
Into high dose group tested material；Measure 20mL high dose group tested materials distilled water is added to be stirred evenly to 60 mL, be made into middle dose group by
Try object；20mL middle dose group tested materials are measured distilled water to be added to stir evenly to 40mL be made into low dose group tested material.
1.2 experimental animal:The cleaning grade ICR health males that Shanghai Slac Experimental Animal Co., Ltd. provides are small
Mouse 160, credit number:SCXK (Shanghai) 2012-0002,18-22g of weight.Animal is divided into four groups (1 group by this experiment
Carry out swimming with a load attached to the body experiment, 2 groups of carry out serum urea experiments, 3 groups of carry out hepatic glycogen experiments, 4 groups of carry out blood lactase acid experiments),
Every group of 40 animals.
1.3 dosage select:The sample Coming-of-Age Day recommended amounts are 2.7g, i.e. (adult's weight is with 60 by 0.045 g/kg BW
Kg is counted), set three dosage groups by recommending 5 times, 10 times, 30 times of dosage: 0.225 g/kg B W 、0 .450 g/kg BW
With 1.35 g/kg BW, distilled water is separately set as solvent control group.
1.4 feeding environment:SPF grades of Animal Lab.s of Disease Control and Prevention Center of Fujian Province, credit number:SYXK (Fujian) 2012-
0008 。
1.5 feed:It is purchased from Shanghai Pu Lu and rises bio tech ltd, credit number:Raise card (2014) 04001 in Shanghai.
1.6 administration route:Animal is weighed daily, and by the 2.0 oral gavages of mL/100g weight give distilled water, it is low,
Middle and high dosage group tested material, continuous 30 days.
1.7 instruments and reagent:Swimming trunk (50 x, 50 x, 40 cm), electronic balance, sheet lead, UI800 UV, visible lights point
Light photometer, 7060 type automatic clinical chemistry analyzer of Hitachi, BECKMAN model TJ-6 centrifuges.Urea reagent, dense sulphur
Acid, the trichloroacetic acid (TCA) of 5 %, 0.1 g/dL of glucose standard, anthrone reagent.
1.8 experimental data statistical methods:Experimental data carries out one-way analysis of variance with SPSS softwares.Through homogeneity of variance
The neat experimental data of inspection, variance is using LSD methods are for statistical analysis, heterogeneity of variance or nonnormal experimental data are first carried out
Variable conversion is counted again.
1.9 experimental method:
1.9.1 Loaned swimming test:
1.9.2 serum urea measures:
After last is to tested material 30min, eyeball is pulled out after 90 min of not swimming with a load attached to the body, rest 60min in the water tank of 30 DEG C of temperature
Adopt about 0.5 mL of whole blood.2000 rpm centrifuge 15 min after putting 4 DEG C of about 3 h of refrigerator, blood clotting, and upper serum is taken to use entirely certainly
Automatic Biochemical Analyzer measures.
1.9.3 hepatic glycogen measures:
After last is to tested material 30min, puts to death animal, liver is taken to be blotted after physiological saline rinses with filter paper, accurately weigh liver
Dirty 100 mg adds in 8mL TCA liquid, and homogenate is poured into centrifuge tube, 15 are centrifuged with 3000 rpm by often pipe homogenate 1min
Supernatant is transferred in another test tube by min, and 1mL supernatants is taken to be put into 10mL centrifuge tubes, and often pipe adds in the ethyl alcohol of 95 %
4mL, abundant mixing to two liquid ask that there are interfaces.Plug is erect stand overnight beyond the Great Wall at room temperature, after precipitation is complete, 3000
Rpm centrifuges 15 min, and 10 min are inverted after removing supernatant, glycogen is dissolved with 2mL distilled water, with anthrone method in 620 nm waves
Long colorimetric estimation hepatic glycogen.
1.9.4 blood lactase acid measures:
After 30 min of last dose, vena ophthalmica 20 uL of blood sampling are immediately placed in 30 DEG C of water went swimmings of water temperature, are put into every 1 min
It one, takes out immediately, dry moisture after 10 min that swim, each 20 μ L that take a blood sample, the 20 μ L that take a blood sample again after 20 min of peace and quiet will be every time
The blood adopted, which is put into the hemolytic agent of 120 μ L, to be vibrated, and after shaken well, measures each blood lactase acid value.
2 results
Influence of 2.1 samples to the mice burden swimming time:
By table 13 as it can be seen that the sample can extend the middle and high dosage group mice burden swimming time, compared with the control group, difference has
Statistical significance (P<0.05 ).
Influence of 2.2 samples to serum urea after mouse movement
By table 14 as it can be seen that the sample can reduce the content of serum urea generated after middle and high dosage group mouse movement, with control group
Compare, the statistically significant (P of difference<0 .05 ) .
Influence of the 2 .3 samples to mouse hepatic glycogen:
By table 15 as it can be seen that the hepatic glycogen content of each dosage group mouse of the sample compared with the control group, difference is not statistically significant
(p> 0.05 ) 。
Influence of 2.4 samples to three time point blood lactase acid area under the curve of mouse:
By table 16 as it can be seen that the sample can reduce middle dose group mouse blood lactase acid area under the curve, compared with the control group, difference has system
Meter learns meaning (P<0.05 ).
Influence of 2.5 samples to mouse weight:
Not statistically significant (the p of original body mass Analysis of variance difference of the sample each group mouse>0.05), i.e. mouse is initial
Weight is more balanced between each group, and at the end of experiment, the end of each group mouse is again through statistics, the not statistically significant (p of difference>
0.05), i.e., the sample on the body weight increase of mouse without influence.It the results are shown in Table 17,18,19,20.
3 brief summaries
Oral gavage gave the health food of the present invention of mouse various dose after 30 days, when as a result can extend mice burden swimming
Between;Reduce the serum urea content generated after mouse movement;Mouse blood lactase acid area under the curve is reduced, and mouse weight is increased
Without influence.Illustrate that the sample has and alleviate physical fatigue effect.
With the rapid development of modern society, social competitiveness is increasing, simultaneously because computer is universal and tight
Study and work opened etc. makes people be pressed by various intentional or unintentional sign stimulus by various tangible or invisible spirit
Power subjects the movement or labour of various large amount of exercise, leads to people often in fatigue even over fatigue state.According to data
Statistics shows the state that China has people more than half to be in chronic fatigue syndrome at present, and 70% is accounted for about in urban population.
Some health care based foods are suitably taken with the improvement of people's health awareness, have been increasingly becoming with disease preventing and treating and keeping fit
At the same time the important means of the countries in the world common people, there is the health food for alleviating physical fatigue effect necessarily to have very big city
Field space.
Health products of the present invention have the healthcare function for alleviating physical fatigue, and functional product social demand person is more, demand
Greatly, launch products provide a kind of selective, trusted, the guaranteed health food of quality, the development of product for demander
Promise well.
The Social benefit and economic benefit of health food is very notable.There is data to show, the application of health food is dropped significantly
The low expenditure of medical expense, and the Meteorological of these medical expenses, significantly larger than health food reduced.Third time country
Health Services investigation and analysis report, directly or indirectly economic loss is as high as estimation Chinese for only 2003 caused by health
800000000000, account for 7% or so of current year GDP.The consumption proportion higher of developed country, the health care expenditures in the U.S. account for the 12% of GDP,
The health care expenditures of Germany account for the 10% of GDP.Over-burden has become a global problem for medical expense.In view of this,
Expert advice should be turned to the health care of preventive medicine, that is, life and the maintenance of health by clinical medicine, and appropriate edible health care product is conducive to
The maintenance of health.According to State Council's regional economic research the survey report of 2002, science edible health food can reduce
500,000,000 yuan of medical expense.There is expert to think, if preventive medicine measure is well-done, disease can reduce half, life longer 10
Year, the medical consumption expenditure can also greatly reduce.Fatigue is a FAQs in daily life.With the development of economy, it lives
The quickening of rhythm, the incidence of chronic fatigue syndrome are gradually increasing.The present invention alleviates physical fatigue health products, not only may be used
So that more people exempt the harmful effect that fatigue is brought, it is more conducive to the reduction of the national medical consumption expenditure.
Specific embodiment
The present invention is described in further detail below by specific embodiment, to help present disclosure is understood.
Embodiment 1
Supplementary material matches：Wilsonii 800g, folium cortex eucommiae 700g, ginseng 100g, cornstarch 70g
Preparation method is：
A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, and irradiation sterilization is spare；
B, wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 12 times of amounts, and second of 10 times of amount is extracted every time
1 hour, filtering was spare；
C, by the Aqueous extracts obtained by step b in 60 degree of temperature, vacuum degree is 0.04 Mpa, under the conditions of be concentrated into 60 degree of heat survey it is opposite
Density is that the concentrate of 1.10-1.20 is spare；
D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 60 degree, and it is standby to obtain dry cream by vacuum degree 0.04Mpa
With；
E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；
F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and cornstarch obtained by step e, mixing is equal
It is even, it is spare to obtain mixed powder；
G, it pelletizes：By the mixed powder dry granulation obtained by step e, particle is spare；
H, capsule charge, packaging, examine to obtain the final product.
Embodiment 2
Supplementary material matches：
Wilsonii 1200g, folium cortex eucommiae 500g, ginseng 300g, cornstarch 50g.
Preparation method is：
A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, and irradiation is spare；
B, it extracts：Wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 8 times of amounts, second of 10 times of amount, every time
Extraction 2 hours, filtering are spare；
C, it concentrates：By the Aqueous extracts obtained by step b in 60 degree of temperature, vacuum degree is concentrated into 60 degree of heat under the conditions of being 0.10Mpa and surveys
Relative density is that the concentrate of 1.10-1.20 is spare；
D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 80 degree, and it is standby to obtain dry cream by vacuum degree 0.10Mpa
With；
E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；
F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and cornstarch obtained by step e, mixing is equal
It is even, it is spare to obtain mixed powder；
G, it pelletizes：By the mixed powder dry granulation obtained by step e, particle is spare；
H, capsule charge, packaging, examine to obtain the final product.
Embodiment 3
Supplementary material matches：
Wilsonii 1000g, folium cortex eucommiae 667g, ginseng 217g, cornstarch 66g.
Preparation method is：
A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, and irradiation sterilization is spare；
B, wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 12 times of amounts, and second of 10 times of amount is extracted every time
1 hour, filtering was spare；
C, by the Aqueous extracts obtained by step b in 60 degree of temperature, vacuum degree is 0.04 Mpa, under the conditions of be concentrated into 60 degree of heat survey it is opposite
Density is that the concentrate of 1.10-1.20 is spare；
D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 60 degree, and it is standby to obtain dry cream by vacuum degree 0.04Mpa
With；
E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；
F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and cornstarch obtained by step e, mixing is equal
It is even, it is spare to obtain mixed powder；
G, it pelletizes：By the mixed powder dry granulation obtained by step e, particle is spare；
H, capsule charge, packaging, examine to obtain the final product.
Embodiment 4
Supplementary material matches：
Wilsonii 1080g, folium cortex eucommiae 658g, ginseng 228g, cornstarch 59g.
Preparation method is：
A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, and irradiation sterilization is spare；
B, wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 10 times of amounts, and second of 10 times of amount carries for the first time
It takes 2 hours, second of extraction 1 hour, filtering is spare；
C, by the Aqueous extracts obtained by step b in 60 degree of temperature, vacuum degree is 0.04 Mpa, under the conditions of be concentrated into 60 degree of heat survey it is opposite
Density is that the concentrate of 1.10-1.20 is spare；
D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 60 degree, and it is standby to obtain dry cream by vacuum degree 0.04Mpa
With；
E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；
F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and cornstarch obtained by step e, mixing is equal
It is even, it is spare to obtain mixed powder；
G, it pelletizes：By the mixed powder dry granulation obtained by step e, particle is spare；
H, capsule charge, packaging, examine to obtain the final product.
Embodiment 5
Supplementary material matches：
Wilsonii 1000g, folium cortex eucommiae 600g, ginseng 200g, starch from sweet potato 50g.
Preparation method is：
A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, and irradiation sterilization is spare；
B, wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 10 times of amounts, and second of 10 times of amount carries for the first time
It takes 2 hours, second of extraction 1 hour, filtering is spare；
C, by the Aqueous extracts obtained by step b in 60 degree of temperature, vacuum degree is 0.04 Mpa, under the conditions of be concentrated into 60 degree of heat survey it is opposite
Density is that the concentrate of 1.10-1.20 is spare；
D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 60 degree, and it is standby to obtain dry cream by vacuum degree 0.04Mpa
With；
E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；
F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and cornstarch obtained by step e, mixing is equal
It is even, it is spare to obtain mixed powder；
G, it pelletizes：By the mixed powder dry granulation obtained by step e, whole grain is packed to get granule.
Embodiment 6
Supplementary material matches：
Wilsonii 1010g, folium cortex eucommiae 620g, ginseng 215g, starch from sweet potato 35g.
Granule is made according to a conventional method to obtain the final product.
Claims (10)
- A kind of 1. health food for relieving physical fatigue containing ginseng, it is characterised in that the weight of each major ingredient of health food Part is as follows：800-1200 parts of wilsonii, 500-700 parts of folium cortex eucommiae, 100-300 parts of ginseng.
- 2. health food according to claim 1, it is characterised in that the parts by weight of each major ingredient of health food are as follows：Thorn 800 parts of slender acanthopanax, 700 parts of folium cortex eucommiae, 100 parts of ginseng.
- 3. health food according to claim 1, it is characterised in that the parts by weight of each major ingredient of health food are as follows：Thorn 1200 parts of slender acanthopanax, 500 parts of folium cortex eucommiae, 300 parts of ginseng.
- 4. health food according to claim 1, it is characterised in that the parts by weight of each major ingredient of health food are as follows：Thorn 1000 parts of slender acanthopanax, 667 parts of folium cortex eucommiae, 217 parts of ginseng.
- 5. health food according to claim 1, it is characterised in that the parts by weight of each major ingredient of health food are as follows：Thorn 1080 parts of slender acanthopanax, 658 parts of folium cortex eucommiae, 228 parts of ginseng.
- 6. according to any health foods of claim 1-5, it is characterised in that also comprising auxiliary material, auxiliary material is the health food Cornstarch or starch from sweet potato.
- 7. health food according to claim 6, it is characterised in that the parts by weight of auxiliary material are 50-70 parts.
- 8. health food as described in any of claims 6, it is characterised in that the preparation formulation of the health food is glue Wafer, tablet, pill, powder, granule or paste.
- 9. health food according to claim 8, it is characterised in that the preparation method of the capsule includes the following steps：A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, irradiation, it is spare that ginseng crushes sterilized powder；B, it extracts：Wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 12 times of amounts, second of 10 times of amount, often Secondary extraction 1 hour, filtering, Aqueous extracts are spare；C, it concentrates：By the Aqueous extracts obtained by step b in temperature 60-80 degree, vacuum degree is concentrated under the conditions of being 0.04-0.10Mpa It is spare that 60 degree of heat survey the concentrate that relative density is 1.10-1.20；D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 60-80 degree, and vacuum degree 0.04-0.10Mpa is obtained It is spare to dry cream；E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and starch obtained by step e, is uniformly mixed, It is spare to obtain mixed powder；G, it pelletizes：By the mixed powder dry granulation obtained by step e, it is spare to obtain particle；H, by obtained by step g grain packing capsule, packaging, examine to obtain the final product.
- 10. health food according to claim 8, it is characterised in that the preparation method of the granule includes following step Suddenly：A, pretreatment of raw material：Ginseng crushes, and crosses 80 mesh sieve, irradiation, it is spare that ginseng crushes sterilized powder；B, it extracts：Wilsonii and folium cortex eucommiae are weighed by formula ratio, water extracts 2 times, for the first time 12 times of amounts, second of 10 times of amount, often Secondary extraction 1 hour, filtering, Aqueous extracts are spare；C, it concentrates：By the Aqueous extracts obtained by step b in temperature 60-80 degree, vacuum degree is concentrated under the conditions of being 0.04-0.10Mpa It is spare that 60 degree of heat survey the concentrate that relative density is 1.10-1.20；D, drying：Concentrate obtained by step c is dried in vacuo, temperature is 60-80 degree, and vacuum degree 0.04-0.10Mpa is obtained It is spare to dry cream；E, it crushes：Dry cream obtained by step d be crushed into 80 mesh sieve, it is spare；F, it mixes：Ginseng obtained by step a is crushed into sterilized powder, adds in comminuted powder and starch obtained by step e, is uniformly mixed, It is spare to obtain mixed powder；G, it pelletizes：By the mixed powder dry granulation obtained by step e, whole grain is packed, is examined to obtain the final product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109674058A (en) * | 2019-02-28 | 2019-04-26 | 江西普正制药有限公司 | A kind of Cortex Eucommiae Gallus domesticlus brisson preparation and preparation method thereof |
| CN111714585A (en) * | 2019-03-18 | 2020-09-29 | 周章镇 | Composition with anti-fatigue effect and preparation method thereof |
| CN111869874A (en) * | 2020-07-30 | 2020-11-03 | 山东贝隆杜仲生物工程有限公司 | Functional food with eucommia ulmoides and ginseng as main materials and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019645A (en) * | 2007-03-08 | 2007-08-22 | 肖樾 | Health food for remitting fatigue and raising hypoxia tolerance and its prepn |
| CN101138419A (en) * | 2007-10-31 | 2008-03-12 | 北京惠中铭捷生物科技有限公司 | Health food having function of improving physical energy and fatigue and its preparation method thereof |
| CN103948039A (en) * | 2014-05-14 | 2014-07-30 | 郭友用 | Health-care food with function of relieving physical fatigue and preparation method of health-care food |
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2016
- 2016-12-01 CN CN201611089527.1A patent/CN108125231A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019645A (en) * | 2007-03-08 | 2007-08-22 | 肖樾 | Health food for remitting fatigue and raising hypoxia tolerance and its prepn |
| CN101138419A (en) * | 2007-10-31 | 2008-03-12 | 北京惠中铭捷生物科技有限公司 | Health food having function of improving physical energy and fatigue and its preparation method thereof |
| CN103948039A (en) * | 2014-05-14 | 2014-07-30 | 郭友用 | Health-care food with function of relieving physical fatigue and preparation method of health-care food |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109674058A (en) * | 2019-02-28 | 2019-04-26 | 江西普正制药有限公司 | A kind of Cortex Eucommiae Gallus domesticlus brisson preparation and preparation method thereof |
| CN111714585A (en) * | 2019-03-18 | 2020-09-29 | 周章镇 | Composition with anti-fatigue effect and preparation method thereof |
| CN111869874A (en) * | 2020-07-30 | 2020-11-03 | 山东贝隆杜仲生物工程有限公司 | Functional food with eucommia ulmoides and ginseng as main materials and application thereof |
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