CN106994157A - A kind of herb fermenting preparation, its preparation method and application for improving performance in layers - Google Patents

A kind of herb fermenting preparation, its preparation method and application for improving performance in layers Download PDF

Info

Publication number
CN106994157A
CN106994157A CN201710184326.8A CN201710184326A CN106994157A CN 106994157 A CN106994157 A CN 106994157A CN 201710184326 A CN201710184326 A CN 201710184326A CN 106994157 A CN106994157 A CN 106994157A
Authority
CN
China
Prior art keywords
parts
preparation
herb fermenting
chinese medicine
weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710184326.8A
Other languages
Chinese (zh)
Inventor
隋利涛
曹亚彬
梁晓彤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harbin Zhongke Biological Engineering Co Ltd
Original Assignee
Harbin Zhongke Biological Engineering Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harbin Zhongke Biological Engineering Co Ltd filed Critical Harbin Zhongke Biological Engineering Co Ltd
Priority to CN201710184326.8A priority Critical patent/CN106994157A/en
Publication of CN106994157A publication Critical patent/CN106994157A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/344—Codonopsis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/487—Psoralea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/533—Leonurus (motherwort)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
    • A61K36/8888—Pinellia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Medical Informatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of herb fermenting preparation, its preparation method and application for improving performance in layers, belong to herb fermenting preparation technique field.The parts by weight of its Chinese medicine each component are constituted：15~25 parts of Radix Codonopsis, 10~20 parts of psoralea corylifolia, 10~20 parts of motherwort, 5~15 parts of the bighead atractylodes rhizome, 5~10 parts of Poria cocos, 5~15 parts of sweet basil, 5~10 parts of honey-fried licorice root, 5~10 parts of the tuber of pinellia, 5~10 parts of dried orange peel.The inventive method choose traditional Chinese medicinal components are rational in infrastructure, ratio is appropriate, the advantages such as the active height of Lactobacillus plantarum, strong stress resistance, the adjustable gut flora of selection, in the presence of the Lactobacillus plantarum selected by the present invention, considerably improve the drug effect of original medicine, beneficial bacterium ratio in enteron aisle is improved, the production performance of laying hen can be improved using the herb fermenting preparation of the present invention.

Description

A kind of herb fermenting preparation, its preparation method and application for improving performance in layers
Technical field
The invention belongs to herb fermenting preparation technique field, and in particular to a kind of herb fermenting of raising performance in layers Preparation, its preparation method and application.
Background technology
The traditional Chinese medical science says, kidney controlling essence storage, is congenital essence first, the formation of embryo, develops using kidney essense as material base, in Jenny is then to promote egg development, with fecundity, when disease and evil invades kidney, can cause deficiency of kidney essence, essence is lost then Infertile, laying hen shows as the phenomenons such as egg production, egg size decline and the thin, soft-shelled egg of eggshell, and breeder then can also go out in addition to above-mentioned phenomenon Existing fertility rate of hatching egg declines；On the other hand, kidney also hides the essence of acquired essence, i.e. water paddy (nutrition), to maintain body vital movement, Enhancing development.Kidney essence deficiency, then filoplume is matt, fluffy and disorderly, and premunition is poor, Body weight loss etc..
Chinese medicine not only has adjustment effect to the secretion of hormone, and some Chinese medicines are also containing similar to the structure of hormone Bioactive substance, with some hormone-like effects, so as to adjust the growth performance of animal, reproductive capability and immunization machine System.
Though traditional Chinese medicine compound preparation on sale can recover laying rate of laying hen in the market, to recover its production in a short time Egg performance has certain difficulty.During the fermentation, can produce some has very powerful decomposition and inversion physical capacity to Chinese medicine Microorganism, and a variety of secondary generations such as cellulase, lignoenzyme, amylase, protease can be produced during growth metabolism Thank to product, the biochemical reaction of complexity is quickly completed at normal temperatures and pressures, than processing means typically physically or chemically more Significantly strengthen and adjust the property of medicine, improve curative effect, reduce toxic side effect.
The content of the invention
For problems of the prior art, it is an object of the invention to provide a kind of natural safety, fast and effectively in Medicine fermentation preparation and the method for preparing the fermentation preparation, while being improved there is provided a kind of having containing the herb fermenting preparation The feed of performance in layers.
In order to solve the above-mentioned technical problem, the present invention is achieved using following technical scheme：
A kind of Chinese medicine composition for improving performance in layers, is prepared from by following components according to parts by weight：Radix Codonopsis 15~25 parts, 10~20 parts of psoralea corylifolia, 10~20 parts of motherwort, 5~15 parts of the bighead atractylodes rhizome, 5~10 parts of Poria cocos, 5~15 parts of sweet basil, process 5~10 parts of radix glycyrrhizae, 5~10 parts of the tuber of pinellia, 5~10 parts of dried orange peel.
On the basis of such scheme, described Chinese medicine composition is prepared from by following components according to parts by weight：Party 20 parts of ginseng, 15 parts of psoralea corylifolia, 15 parts of motherwort, 10 parts of the bighead atractylodes rhizome, 6 parts of Poria cocos, 10 parts of sweet basil, 6 parts of honey-fried licorice root, 6 parts of the tuber of pinellia, dried orange peel 6 parts.
A kind of herb fermenting preparation, it by traditional Chinese medicine ingredients mixed with Lactobacillus plantarum bacterium solution after through fermentation be made, it is described in Medicine composition is by weight：15~25 parts of Radix Codonopsis, 10~20 parts of psoralea corylifolia, 10~20 parts of motherwort, the bighead atractylodes rhizome 5~15 Part, 5~10 parts of Poria cocos, 5~15 parts of sweet basil, 5~10 parts of honey-fried licorice root, 5~10 parts of the tuber of pinellia, 5~10 parts of dried orange peel.
On the basis of such scheme, the traditional Chinese medicine ingredients are by weight：20 parts of Radix Codonopsis, psoralea corylifolia 15 Part, 15 parts of motherwort, 10 parts of the bighead atractylodes rhizome, 6 parts of Poria cocos, 10 parts of sweet basil, 6 parts of honey-fried licorice root, 6 parts of the tuber of pinellia, 6 parts of dried orange peel.
On the basis of such scheme, Lactobacillus plantarum content >=10 in the bacterium solution8CFU/mL。
On the basis of such scheme, the preparation method of the herb fermenting preparation, step is as follows：
(1) by traditional Chinese medicinal components are measured, crush, cross 60 eye mesh screens, it is standby；
(2) each component after crushing is well mixed, adds the water of traditional Chinese medicine ingredients gross weight 80%, stir after 100 DEG C of steam sterilizing 30min, are cooled to 20-30 DEG C；
(3) bacterium solution is added into the medicine after cooling, bacterium solution consumption accounts for 10~15% of total amount after addition；It is placed in unidirectional row In gas polybag, fermented 30 days under the conditions of 20 DEG C~30 DEG C.
Above-mentioned one-way exhaust polybag is Patent No.：200920100585.9 anaerobic fermentation feed, Chinese medicine and add Plus the one-way exhaust bag of agent.
On the basis of such scheme, the bacterium solution is made by following methods：
Lactobacillus plantarum strain is inoculated in activation medium and carries out activation culture, cultivation temperature is 37 DEG C, time 24h； The strain activated is inoculated in fermentation tank, 37 DEG C~43 DEG C of temperature, time 24h~36h is controlled.
On the basis of such scheme, the activation medium and the fermentation used medium are：MRS culture mediums, into It is divided into (g/L)：Peptone 10.0, meat extract 10.0, yeast extract 5.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, hydrogen citrate two Ammonium 2.0, sodium acetate 5.0, magnesium sulfate 0.58, manganese sulfate 0.28, cysteine 0.5, Tween 80 1.0, adjust pH6.2-6.4.
On the basis of such scheme, the herb fermenting formulation application is in the production performance for improving laying hen.
It is a kind of that there is the feed for improving performance in layers, it is herb fermenting system prepared by the above method of being added in feed Agent, addition is 2kg/ tons.
The raising performance in layers Chinese medicine composition of the present invention, the property of medicine drug effect of each Chinese medicine is as follows：
Radix Codonopsis：Main component is alkaloid, saponin, protein, starch, vitamin B1, vitamin B2 etc., with bowl spares benefit Gas, enrich blood, be depressured, eliminating phlegm and relieving cough the effects such as.
Psoralea corylifolia：For the ripe dry seed of pulse family annual herb plant psoralea corylifolia.Cultivation, also has wild.Containing Psoralen Fat B prime, is natural chalcone；Contain a variety of Coumarins materials, predominantly psoralen and isopsoralene again.Have It is warming spleen and stopping diarrha, tonifying kidney and strengthening yang, the effect of astringent sperm contracting urine.
Motherwort：For herb on the dry ground of labiate motherwort or great Hua motherworts.It is wild.Containing motherwort Alkali first, leonurine second, separately containing compositions such as stachydrine, potassium chloride, laurate, oleic acid.The work(such as the stasis of blood are dissipated with promoting blood circulation for regulating menstruation, promoting circulation of blood Effect.
The bighead atractylodes rhizome：For the dry rhizome of the composite family herbaceos perennial bighead atractylodes rhizome.Mostly cultivate, also there is wild.Main component is Volatile oil, wherein predominantly atractylol, the material such as another ketone containing the bighead atractylodes rhizome, retinoid.With invigorating the spleen and benefiting qi, the work(such as eliminate dampness and have diuretic effect Effect.
Poria cocos：To post the dry sclerotia of bacterium Polyporaceae Poria cocos.Wild survivor's multiparasitization is in the root of Japanese red pine or raw true masson pine Portion, shiploads of merchandise is artificial cultivation.Mainly contain pachymose, pachymic acid, protein, fat, lecithin, histidine, choline, wheat The composition such as angle sterol and sylvite, primary efficacy is clearing damp and promoting diuresis, beneficial spleen calming heart.
Sweet basil：For annual upright draft.For cultivated plant, also have wild, be born in hillside roadside, bushes more.Contain Volatile oil 0.02~0.04%, main component be ocimenum, australene, 1, 8-Cineole, linalool, Geraniol, limonene, Carene-3, estragole, Eugenol, methyleugenol, anisole, methyl cinnamate, hexene -8- alcohol -1, octanone -3 And furfural etc..The effects such as with analgesic, detumescence, the scattered stasis of blood, removing toxic substances.
Radix glycyrrhizae：Alias RADIX GLYCYRRHIZAE, is the dry root and root-like stock of leguminous perennial herb radix glycyrrhizae and glycyrrhiza glabra etc. (reed head), it is mostly wild.Containing glycyrrhizin, liquiritigenin, glucose, mannitol, malic acid, asparagine etc. it is main into Point, with invigorating the spleen and benefiting qi, it is clearing heat and detoxicating, the effects such as moisten the lung and relieve the cough.Honey-fried licorice root is the radix glycyrrhizae baked with honey, is commonly called as processing, is exactly Honey is put middle honey is smelt in pot, used slow fire instead plus licorice piece is stirred uniformly, 3~5min takes the dish out of the pot, put 60 DEG C of barn or baking oven Take out, cool when being dried to tack-free.
The tuber of pinellia：For the perennial herblet of Araeceae, the tuber of pinellia rubs the dry tuber for removing crust with the hands.Cultivation is wild.Contain The composition such as B- sitosterol and its glycoside sitosterol, trace volatile oil, phytosterol, saponin, acidity material, biological alkali. The effect of with anti-vomiting, removing dampness and eliminating phlegm, mass dissipating and swelling eliminating.
Dried orange peel：Mainly containing hesperidine, mysoinositol, volatile oil (main component is d-limonen) and vitamin B1 etc. into Point.With stomach invigorating, wind dispelling, preventing or arresting vomiting, only oh, eliminating the phlegm the effects such as.
Wherein described Lactobacillus plantarum strain is Lactobacillus plantarum plant subspecies (Lactobacillus plantarum Subsp.Plantarum), numbering AS1.557, from Institute of Microorganism, Academia Sinica.
The technical principle of the present invention：
In side Radix Codonopsis tonifying middle-Jiao and Qi and can blood-nourishing be monarch, psoralea corylifolia, motherwort, the yang-tonifying of bighead atractylodes rhizome principal drug assistance warm kidney, to train kidney Sun this, promoting blood circulation, it is drying damp and strengthening spleen be minister, Poria cocos inducing diuresis with bland drug, sweet basil restore menstrual flow and invigorate blood circulation, dampness elimination helps digestion, make spleen be damp evil It is stranded for adjutant, dried orange peel is added again to strengthen the effect of invigorating the spleen qi-regulating, plus tuber of pinellia regulating qi-flowing for harmonizing stomach, eliminating phlegm and lowering adverse QI is adjutant, honey-fried licorice root Sweet temperature QI invigorating and can coordinating the drug actions of a prescription to make medicine.Full side has QI invigorating and stomach, and the work(of promoting the circulation of qi middle benefit gas, nature and flavor are gentle, all medicines share, mark Originally it is simultaneous to control.
Herb fermenting preparation of the present invention has the characteristics that：(1) Chinese prescription fill blood, strengthening the spleen and stomach, liver and kidney benefiting, pass through Quality of laying eggs etc. is improved to approach such as neuroendocrine regulations, performance in layers can be effectively improved.(2) present invention is selected Lactobacillus plantarum can tens kinds of extracellular proteinase, cellulase, hemicellulase, pectase, amylase etc. in metabolic process Ectoenzyme enters culture medium, and what these enzymes had can form new compound, what is had can decompose by drug ingedient decomposition and inversion The compact texture such as cellulose, hemicellulose, pectic substance, lignin in plant cell wall, promotes active constituents of medicine to greatest extent Release；(2) Lactobacillus plantarum and its metabolite that the present invention is selected effectively supplement or strengthened the function of original medicine； (3) new active material is generated after Chinese medicine is converted through Lactobacillus plantarum, and brings new health care, prevent or controls treatment functions.
It is birds in vivo under hormone and trophic factor collective effect to lay eggs, and is regulated and controled by hypothalamic-pituitary-ovarian axis Complex physiologic process.Effects of Bu Gu fat has an effect for promoting ovarian function, estrogen contained by Chinese medicine sweet basil can by acceptor and Feedback mechanism promotes follicular development and the secretion of reproductive hormone in ovary, and to promote to ovulate and perfect luteal function, Chinese medicine benefit is female Grass is enriched blood, excited uterus, ovulation rate.The secretion of testosterone can be significantly reduced using the herb fermenting preparation of the present invention, is improved The secretion of progesterone and estradiol, so as to improve the laying rate of laying hen, reduce feedstuff-egg ratio and improve the quality of egg；This Outside, the addition of herb fermenting preparation of the present invention can also reduce the content of yolk cholesterol.
The inventive method choose traditional Chinese medicinal components are rational in infrastructure, ratio appropriate, the Lactobacillus plantarum of selection it is active it is high, The advantages such as strong stress resistance, adjustable gut flora, in the presence of the Lactobacillus plantarum selected by the present invention, are considerably improved The drug effect of original medicine, improves beneficial bacterium ratio in enteron aisle, and laying hen can be improved using the herb fermenting preparation of the present invention Production performance.
Embodiment
Below in conjunction with preferred embodiment, to according to the embodiment of the invention provided, details are as follows：
Embodiment 1
The preparation of bacterium solution：
The Lactobacillus plantarum strain of the present invention is inoculated in into activation medium to be cultivated, cultivation temperature is 37 DEG C, time 24h；The strain activated is inoculated in fermentation tank, 37 DEG C~43 DEG C of temperature is controlled, time 24h~36h prepares zymotic fluid.
Wherein described Lactobacillus plantarum strain is Lactobacillus plantarum plant subspecies (Lactobacillus plantarum Subsp.Plantarum), numbering AS1.557, from Institute of Microorganism, Academia Sinica.
The activation medium is：MRS culture mediums, composition is (g/L)：Peptone 10.0, meat extract 10.0, yeast extract 5.0th, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0, magnesium sulfate 0.58, manganese sulfate 0.28, Cysteine 0.5, Tween 80 1.0, adjust pH6.2-6.4.
Fermentation used medium be：MRS culture mediums, composition is ibid.
After the completion of fermentation, content >=10 of bacterium in bacterium solution8CFU/mL。
Embodiment 2
A kind of herb fermenting preparation for improving performance in layers, the parts by weight of its each component are：20 parts of Radix Codonopsis, Psoralen 15 parts of fat, 15 parts of motherwort, 10 parts of the bighead atractylodes rhizome, 6 parts of Poria cocos, 10 parts of sweet basil, 6 parts of honey-fried licorice root, 6 parts of the tuber of pinellia, 6 parts of dried orange peel.
Specific preparation method is as follows：
(1) by traditional Chinese medicinal components are measured, crush, cross 60 eye mesh screens, it is standby；
(2) each component after crushing is well mixed, adds the water of traditional Chinese medicine ingredients gross weight 80%, stir after 100 DEG C of steam sterilizing 30min, are cooled to 20-30 DEG C；
(3) bacterium solution is added into the medicine after cooling, bacterium solution consumption accounts for 10~15% of total amount after addition；It is placed in unidirectional row In gas polybag, fermented 30 days under the conditions of 20 DEG C~30 DEG C.
Above-mentioned one-way exhaust polybag is Patent No.：200920100585.9 anaerobic fermentation feed, Chinese medicine and add Plus the one-way exhaust bag of agent.
Embodiment 3
A kind of herb fermenting preparation for improving performance in layers, the parts by weight of its each component are：15 parts of Radix Codonopsis, Psoralen 10 parts of fat, 10 parts of motherwort, 5 parts of the bighead atractylodes rhizome, 5 parts of Poria cocos, 5 parts of sweet basil, 5 parts of honey-fried licorice root, 5 parts of the tuber of pinellia, 5 parts of dried orange peel.
Preparation method is same as Example 2.
Embodiment 4
A kind of herb fermenting preparation for improving performance in layers, the parts by weight of its each component are：25 parts of Radix Codonopsis, Psoralen 20 parts of fat, 20 parts of motherwort, 15 parts of the bighead atractylodes rhizome, 10 parts of Poria cocos, 15 parts of sweet basil, 10 parts of honey-fried licorice root, 10 parts of the tuber of pinellia, 10 parts of dried orange peel.
Preparation method is same as Example 2.
First, the test of pesticide effectiveness：
1.1 experiment prescriptions
1.1.1 the non-fermented Chinese medicine preparation group that prepared by traditional handicraft (is surpassed the medicine selected by the embodiment of the present invention 2 Crushing of Ultrafine, is crushed to 700 mesh, is well mixed)
1.1.2 2 groups of embodiment
1.1.3 3 groups of embodiment
1.1.4 4 groups of embodiment
1.1.5 blank control group
1.2 zooperies are designed
Experiment uses single-factor randomized complete-block design, and 48 weeks body weight health sea orchid close with laying rate brown is laid eggs Chicken 600, is randomly divided into 5 processing, adds Ultramicro-powder, the herb fermenting preparation of embodiment 2, embodiment 3 respectively in feed Herb fermenting preparation and embodiment 4 herb fermenting preparation, addition be 2kg/ tons, separately set one group of blank control group, other Experimental condition is completely the same.Each 6 repetitions of processing, each repeat 20 chickens.It is each to handle search for food respectively a kind of feed, prerun 1 week phase, positive 30 days phases of examination.
1.3 experiment diets
Need to prepare Basic drawing with reference to NRC (1994) laying hen nutrition, diet composition and trophic level are shown in Table 1.
The Basic drawing of table 1 is constituted and trophic level (air bells dry basis)
Note：1) every kilogram of diet contains：VA 12000IU, VD33000IU, VE 20IU, VK 2m g, VB12 0.015mg, VB11.5mg, riboflavin riboflavin 4.5mg, VB63.0mg, biotin biotin 0.1mg, folic acid Folic acid 0.5mg, nicotinic acid niacin 20mg, pantothenic acid pantothenic acid 10.0mg.
2) every kilogram of diet contains：Mn 60.0mg, Zn 50.0mg, Fe 25.0mg, Cu 5.0mg, I 0.5mg.
3) metabolic energy is calculated value, and remaining is measured value.
1.4 feeding management
Using the 3 layers of three-dimensional cage of semi open model hen house, connected 5 chicken coops (45cm × 30cm × 30cm) are often arranged as 1 Individual repetition, it is each to repeat to be evenly distributed on upper, middle and lower cage frame per 4 chickens of cage.
Using nature plus artificially feed, daily constant illumination 16h (automatic illuminating control system), intensity is 14LX.Feeding Dry mash, free choice feeding, daily 08:00 and 14:30 each feedings 1 time, refining 4 times；Nipple-shaped drinker, free water.By just Chang Chengxu prevents epidemic, and personal management picks up egg 2 times daily；Carry out disinfection and clear up weekly chicken manure 1 time, routine immunization.
1.5 testing indexs and method
1.5.1 egg-laying test
To be repeated as the daily egg number of unit record (including soft-shelled egg, check), day egg production, dead chicken number, weekly Record the feed consumption rate of laying hen.Finally calculate laying rate, daily ingestion amount, average egg weight, feedstuff-egg ratio.
1.5.2 Egg Quality is determined
Eggshell strength, shell thickness, albumen height, yolk color and hangh unit are produced using ORKA companies of Israel Serial egg quality analyzer is measured, and the egg shape index analyzer that egg shape index is produced using Japanese fuji level ground company is surveyed It is fixed, every 2 weeks 1 time, totally 4 times.
1.5.3 enteric microorganism is analyzed
In before off-test early morning on same day feeding, often repeating to butcher 2 (totally 60) chickens, 5% bromogeramine solution leaching Steep 5min, whole body sterilization；Under aseptic condition, caecum stage casing 10cm is cut, test in laboratory wherein chyme is taken back at ligation two ends Bifidobacterium, Escherichia coli and Bacillus acidi lactici content.The quantity of flora is represented with lgCFU/g cecal contents.
1.5.4 reproductive hormone assay
It is each to repeat to randomly select after venous blood collection 3mL under 5 chickens, wing, standing respectively in the 15d and 30d of experiment 3000r/min centrifuges 10min, and to be placed on -20 DEG C of refrigerators standby for serum after separation.With automatic clinical chemistry analyzer (Hitachi 7600-020) determine testosterone (T), progesterone (P) and estradiol (E2) content in serum.
1.5.5 the measure of shell egg and cholesterol level of vitellus
Respectively in the 15d and 30d of experiment, every group is randomly selected 10 pieces of eggs, mark group, date, is placed in 4 DEG C of refrigerators Preserve, for determining shell egg and cholesterol level of vitellus.Every piece of egg is weighed before measure, the reference of the yellow and white separation method (research that Different Alfalfa grass meal level influences on laying hen cholesterol level of vitellus, Wang Chengzhang, Yang Yuxin, Hu Xi such as Wang Chengzhang Peak etc., Acta Prataculture, 2005,14 (2)：Method 76-83).To yolk correct amount, and calculate yolk relative weight.
Yolk relative weight (%)=(yolk weight/egg size) × 100%
Egg cholesterol amount is represented with 2 indexs of cholesterol level of vitellus and shell egg cholesterol level.Wherein, yolk Cholesterol level refers to the content of cholesterol in every gram of yolk, and shell egg cholesterol level refers to containing for cholesterol in every piece of egg Amount.Using GBT 22220-2008《The measure high performance liquid chromatography of Food Cholesterol》Prescriptive procedure is measured.
1.5.6 data analysis
All gathered datas carry out early stage processing using Excel softwares, then carry out statistical analysis, LSD with SPSS19.0 Multiple range test is carried out, is as a result represented using mean+SD.
2nd, result and analysis
Respectively influence of the experiment component to Layer Production Performance of 2.1 experimental periods, is shown in Table 2.
Respectively influence of the experiment component to performance in layers of the experimental period of table 2
Note：Same column shoulder marking-up parent phase is with the not notable (P of expression difference>0.05), the different lowercase letter indication differences of shoulder mark show Write (P<0.05), the different capitalizations of shoulder mark represent difference extremely significantly (P<0.01).
Test chicken is managed during experiment everywhere in good condition, defecation is normal, no death.
As shown in Table 2, experimental period average egg weight and daily ingestion amount, there was no significant difference between each test group and control group (P >0.05).Laying rate Ultramicro-powder group is higher than control group but there was no significant difference (P>0.05), 2.46% is improved than control group；It is real Apply example 2,3 and 4 groups significantly improve 6.32%, 6.26% and 6.23% (P than control group respectively<0.05) Ultramicro-powder, is compared respectively Group significantly improves 3.77%, 3.71% and 3.68% (P<0.05).Day egg production Ultramicro-powder group is improved than control group 1.86% (P>0.05)；Embodiment 2,3 and 4 groups significantly improve 6.38%, 5.91% and 5.83% (P than control group respectively< 0.05)；Respectively 4.44%, 3.98% and 3.90% (P is significantly improved than Ultramicro-powder group<0.05).Each test group of daily ingestion amount There was no significant difference between control group (P>0.05).Feedstuff-egg ratio embodiment 2,4 groups significantly reduced respectively than control group pole 4.87% and 4.87% (P<0.01).
Respectively influence of the experiment component to Egg Production of Laying Hens quality of 2.2 experimental periods, is shown in Table 3.
Respectively influence of the experiment component to Egg Production of Laying Hens quality of the experimental period of table 3
Note：Same column shoulder marking-up parent phase is with the not notable (P of expression difference>0.05), the different lowercase letter indication differences of shoulder mark show Write (P<0.05), the different capitalizations of shoulder mark represent difference extremely significantly (P<0.01).
As shown in Table 3, there was no significant difference between experimental period shell thickness and each test group of eggshell strength and control group (P> 0.05).2 groups of egg shape index embodiment is significantly higher than control group (P<0.05), there was no significant difference with control group for other test groups (P>0.05).Albumen height, embodiment 2,3 and 4 groups of poles are significantly higher than control group (P<0.01) it is, poor without conspicuousness with Ultramicro-powder group Different (P>0.05), there was no significant difference between Ultramicro-powder group and control group (P>0.05).Each test group of Hough unit and control group Between there was no significant difference (P>0.05).
Influence of the 2.3 each experiment components to laying hen cecum microorganisms, is shown in Table 4.
Respectively influence of the experiment component to laying hen cecum microorganisms of table 4
Note：Same column shoulder marking-up parent phase is with the not notable (P of expression difference>0.05), the different lowercase letter indication differences of shoulder mark show Write (P<0.05), the different capitalizations of shoulder mark represent difference extremely significantly (P<0.01).
As shown in Table 4, Escherichia coli quantity embodiment 2,3 and 4 groups of poles are substantially less than control group (P<0.01)；Ultramicro-powder group With control group there was no significant difference (P>0.05).Bacillus acidi lactici quantity embodiment 2,3 and 4 groups be significantly higher than control group (P< 0.05), Ultramicro-powder group and control group there was no significant difference (P>0.05).Bifidobacteria embodiment 2,4 groups of poles are significantly higher than Control group (P<0.01), 3 groups of Ultramicro-powder group and embodiment and control group there was no significant difference (P>0.05).
Influence of the 2.4 herb fermenting preparations to peak later stage Laying-hen Serum reproductive hormone content of laying eggs, is shown in Table 5.
Influence of the herb fermenting preparation of table 5 to peak later stage Laying-hen Serum reproductive hormone content of laying eggs
Note：Same column shoulder marking-up parent phase is with the not notable (P of expression difference>0.05), the different lowercase letter indication differences of shoulder mark show Write (P<0.05), the different capitalizations of shoulder mark represent difference extremely significantly (P<0.01).
As shown in Table 5, test the 15th day, each test group of testosterone concentration is below control group, wherein embodiment 2,4 groups extremely show Write and be less than control group (P<0.01), respectively than control group reduction by 9.36% and 8.36%；Compared with the 15th day, testosterone contains within the 30th day Amount decreases, and embodiment 2,3 and 4 groups of other poles of ratio control component significantly reduce 29.46%, 12.45% and 29.04%. Test the 15th day, pole significantly improves (P to each test group progesterone content compared with control group<0.01), Ultramicro-powder group, embodiment 2, 3 and 4 groups significantly improve 21.07%, 39.46%, 21.34% and 33.82% respectively；Test the 30th day, each test group progesterone Content there was no significant difference (P>0.05).Test the 15th, 30 days, each test group estradiol content pole is significantly higher than control group (P <0.01), the 15th day, Ultramicro-powder group, embodiment 2,3 and 4 groups respectively pole significantly improve 13.79%, 52.05%, 34.42% With 35.83%；30th day, pole significantly improved 4.49%, 6.28%, 4.90% and 5.46% respectively.
Influence of the 2.5 herb fermenting preparations to shell egg and cholesterol level of vitellus, is shown in Table 6.
Influence of the herb fermenting preparation of table 6 to shell egg and cholesterol level of vitellus
Note：Shoulder marking-up parent phase go together with the not notable (P of expression difference>0.05), the different lowercase letter indication differences of shoulder mark show Write (P<0.05), the different capitalizations of shoulder mark represent difference extremely significantly (P<0.01).
As shown in Table 6, experiment the 15th, 30 days, there was no significant difference between each test group of yolk relative weight and control group. Test the 15th day, each test group cholesterol level of vitellus pole is substantially less than control group (P<0.01), Ultramicro-powder group, embodiment 2, 3 and 4 groups respectively than control group reduction by 8.44%, 14.66%, 13.18% and 13.35%；Test the 30th day, yolk cholesterol contains Amount embodiment 2,3 and 4 groups significantly reduce 15.40%, 11.51% and 14.98% (P than control group pole respectively<0.01)；Ultra micro Powder group reduces 8.69% (P than control group>0.05).Test the 15th, 30 days, embodiment 2,3 and 4 groups of shell egg cholesterol levels point Do not significantly reduce 15.24%, 10.41%, 11.31% and 14.74% than control group pole, 14.56%, 11.80% (P< 0.01)；Ultramicro-powder group does not reduce 2.30% (P than control component>0.05) with 8.18% (P<0.01).
3rd, pharmacological toxicity is tested
3.1 acute toxicity test data
Detection reagent：Serum AST, ALT detection kits build up Bioengineering Research Institute purchased from Nanjing.
Detection device
Micro-200 semiautomatic biochemistry detectors, assay balance, disscting instrument, syringe, test tube etc..
3.2 test method
The ICR mouse 100 that body weight is 18~22g are chosen, after being raised through 3 days adaptability, 5 groups are divided at random, and (male and female are each Half), Ultramicro-powder group, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment and blank control group, every group each 20；Blank control group Gavage physiological saline, the Chinese medicine composition that Ultramicro-powder used in Ultramicro-powder group is selected in the embodiment of the present invention 2 carries out ultramicro grinding and obtained The Ultramicro-powder arrived；Ultramicro-powder group is gavaged Ultramicro-powder is soluble in water, and 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment respectively will be each The fermentation preparation of embodiment preparation is soluble in water to be gavaged, Ultramicro-powder group, 2 groups of embodiment, 3 groups of embodiment, the medicine of 4 groups of embodiment Addition is 0.1g/mL, 0.4mL/ times, three times a day, continuous 7 days.
3.3 testing indexs and method
(1) clinicing symptom observation：The state of mind, the diet situation of daily viewing test mouse, dead feelings of being fallen ill per day entry Condition.
(2) pathological observation：Cut open inspection observation internal organ change is carried out for dead mouse.
(3) growth indexes are observed：Routine weighing is carried out to experiment mouse；Off-test, slaughter mouse weigh in, internal organs weight, Organ index is determined：Cut open inspection rat, core dirty, spleen, liver, kidney, testis, ovary, is weighed, and internal organs are calculated according to the following formula and are referred to Number：Organ index=internal organs weight/body weight × 100.
(4) change in liver function is observed：After off-test, mouse is slaughtered, takes blood to separate serum, AST, ALT is determined.
3.4 data process＆analysis
All gathered datas carry out early stage processing using Excel softwares, then carry out statistical analysis, LSD with SPSS19.0 Multiple range test is carried out, is as a result represented using mean+SD.
3.5 clinicing symptom observation
Control group and test group mouse, the state of mind and diet desire, to external world respond have no obvious exception.Each group mouse Morbidity and death condition such as table 7 below.
The mouse of table 7 falls ill and death condition
Packet Sum Dead (only) The death rate (%)
Ultramicro-powder 20 0 0
2 groups of embodiment 20 0 0
3 groups of embodiment 20 0 0
4 groups of embodiment 20 0 0
Control group 20 0 0
Pathological observation：Control group and test group, progress cut open inspection, which is observed, after mouse is slaughtered has no the obvious pathology of internal organs Change.
Growth indexes are observed：Off-test, slaughters that mouse weighs in, internal organs weight analysis are shown in Table 8.
The mouse internal organs weight analysis of table 8
Packet Liver index Cardiac index Renal index Lungs index Index and spleen index
Ultramicro-powder 54.05±1.30a 4.36±0.12a 13.44±0.22a 6.69±0.43a 4.61±0.55a
2 groups of embodiment 54.21±1.14a 4.71±0.20a 13.49±0.18a 6.81±0.35a 4.96±0.45a
3 groups of embodiment 54.18±1.32a 4.66±0.25a 13.50±0.23a 6.74±0.38a 5.01±0.40a
4 groups of embodiment 54.09±1.24a 4.72±0.19a 13.47±0.22a 6.75±0.33a 4.92±0.42a
Control group 53.92±1.43a 4.30±0.18a 13.36±0.19a 6.61±0.36a 4.52±0.51a
Note：Same column shoulder marking-up parent phase represents difference not significantly (P with or without letter>0.05), the female different small letters of shoulder marking-up Matrix shows significant difference (P<0.05).
As shown in Table 8, each organ index analysis, there was no significant difference between each test group and control group (P>0.05).
Change in liver function is observed
After off-test, mouse is slaughtered, takes blood to separate serum, AST (glutamic-oxalacetic transaminease), ALT (third turn of ammonia of paddy is determined Enzyme) it is shown in Table 9.As a result show that each group of data difference compared with blank control group is not notable, all in normal range (NR).
Table 9 AST, ALT measurement result
Packet Sum ALT AST
Ultramicro-powder 20 39.04±2.55a 105.46±4.62a
2 groups of embodiment 20 39.22±2.07a 105.74±4.19a
3 groups of embodiment 20 39.18±1.99a 105.57±3.36a
4 groups of embodiment 20 39.23±2.11a 105.69±4.03a
Control group 20 39.08±2.25a 105.41±5.05a
Note：Same column shoulder marking-up parent phase represents difference not significantly (P with or without letter>0.05), the female different small letters of shoulder marking-up Matrix shows significant difference (P<0.05).
3.6 long term toxicity test data
The fermentation preparation of the present invention prepared according to embodiment 2,20g/kg, 100g/kg give to 1 monthly age ICR mouse continuous gavage Medicine 1 month, the fur of animal, the colour of skin, ingest, activity, urine, feces etc. it is without exception；The blood such as blood routine, the Liver and kidney function of animal Liquid biochemical indicator and the main organs index of animal are in normal range (NR), and normal kinetic indifference；Histopathologic examination's table Bright, the important organ such as the heart, liver, spleen, lung, kidney, adrenal gland, tracheae, testis, ovary is without pathological change.Checked after being discontinued two weeks with Upper indices react without overt toxicity, show no cumulative toxicity.
The above described is only a preferred embodiment of the present invention, being not the limitation for making other forms to the present invention, appoint What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc. Imitate embodiment.But it is every without departing from technical solution of the present invention content, the technical spirit according to the present invention is to above example institute Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.

Claims (10)

1. a kind of Chinese medicine composition for improving performance in layers, it is characterised in that：Prepared by following components according to parts by weight Form：15~25 parts of Radix Codonopsis, 10~20 parts of psoralea corylifolia, 10~20 parts of motherwort, 5~15 parts of the bighead atractylodes rhizome, 5~10 parts of Poria cocos, sweet basil 5 ~15 parts, 5~10 parts of honey-fried licorice root, 5~10 parts of the tuber of pinellia, 5~10 parts of dried orange peel.
2. Chinese medicine composition according to claim 1, it is characterised in that：By following components according to parts by weight preparation Into：20 parts of Radix Codonopsis, 15 parts of psoralea corylifolia, 15 parts of motherwort, 10 parts of the bighead atractylodes rhizome, 6 parts of Poria cocos, 10 parts of sweet basil, 6 parts of honey-fried licorice root, the tuber of pinellia 6 Part, 6 parts of dried orange peel.
3. a kind of herb fermenting preparation, it is characterised in that：It by traditional Chinese medicine ingredients mixed with Lactobacillus plantarum bacterium solution after through fermentation make Into the traditional Chinese medicine ingredients are by weight：15~25 parts of Radix Codonopsis, 10~20 parts of psoralea corylifolia, 10~20 parts of motherwort, 5~15 parts of the bighead atractylodes rhizome, 5~10 parts of Poria cocos, 5~15 parts of sweet basil, 5~10 parts of honey-fried licorice root, 5~10 parts of the tuber of pinellia, 5~10 parts of dried orange peel.
4. herb fermenting preparation according to claim 3, it is characterised in that：The traditional Chinese medicine ingredients are by weight For：20 parts of Radix Codonopsis, 15 parts of psoralea corylifolia, 15 parts of motherwort, 10 parts of the bighead atractylodes rhizome, 6 parts of Poria cocos, 10 parts of sweet basil, 6 parts of honey-fried licorice root, the tuber of pinellia 6 Part, 6 parts of dried orange peel.
5. the herb fermenting preparation according to claim 3 or 4, it is characterised in that：Lactobacillus plantarum content in the bacterium solution ≥108CFU/mL。
6. according to the preparation method of any one of the claim 3-5 herb fermenting preparations, it is characterised in that：Step is as follows：
(1) by traditional Chinese medicinal components are measured, crush, cross 60 eye mesh screens, it is standby；
(2) each component after crushing is well mixed, adds the water of traditional Chinese medicine ingredients gross weight 80%, stir after 100 DEG C Steam sterilizing 30min, is cooled to 20-30 DEG C；
(3) bacterium solution is added into the medicine after cooling, bacterium solution consumption accounts for 10~15% of total amount after addition；It is placed in one-way exhaust modeling In pocket, fermented 30 days under the conditions of 20 DEG C~30 DEG C.
7. the preparation method of Chinese medicine preparation according to claim 6, it is characterised in that：The bacterium solution is made by following methods：
Lactobacillus plantarum strain is inoculated in activation medium and carries out activation culture, cultivation temperature is 37 DEG C, time 24h；Will be living The strain changed is inoculated in fermentation tank, controls 37 DEG C~43 DEG C of temperature, time 24h~36h.
8. the preparation method of herb fermenting preparation according to claim 7, it is characterised in that：The activation medium and described Fermentation used medium be：MRS culture mediums, composition is (g/L)：Peptone 10.0, meat extract 10.0, yeast extract 5.0, glucose 20.0th, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0, magnesium sulfate 0.58, manganese sulfate 0.28, cysteine 0.5th, Tween 80 1.0, adjust pH 6.2-6.4.
9. according to the application of any one of the claim 3-8 herb fermenting preparations, it is characterised in that：Life for improving laying hen Produce performance.
10. a kind of have the feed for improving performance in layers, it is characterised in that：Addition such as claim 6 method in feed The herb fermenting preparation of preparation, addition is 2kg/ tons.
CN201710184326.8A 2017-03-24 2017-03-24 A kind of herb fermenting preparation, its preparation method and application for improving performance in layers Pending CN106994157A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710184326.8A CN106994157A (en) 2017-03-24 2017-03-24 A kind of herb fermenting preparation, its preparation method and application for improving performance in layers

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710184326.8A CN106994157A (en) 2017-03-24 2017-03-24 A kind of herb fermenting preparation, its preparation method and application for improving performance in layers

Publications (1)

Publication Number Publication Date
CN106994157A true CN106994157A (en) 2017-08-01

Family

ID=59431531

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710184326.8A Pending CN106994157A (en) 2017-03-24 2017-03-24 A kind of herb fermenting preparation, its preparation method and application for improving performance in layers

Country Status (1)

Country Link
CN (1) CN106994157A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107467404A (en) * 2017-10-23 2017-12-15 潍坊华光数码设备有限公司 A kind of feed addictive for laying hen health care and preparation method thereof
CN110897053A (en) * 2019-12-20 2020-03-24 姜丹 Fly-repelling traditional Chinese medicine fermented feed for improving egg quality of laying hens and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101401921A (en) * 2008-10-30 2009-04-08 河南省龙腾高科实业有限公司 Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof
CN102232474A (en) * 2011-04-15 2011-11-09 北京康华远景科技有限公司 Disease-resistant growth promoter for aquatic animals and preparation method thereof
CN105767587A (en) * 2016-04-29 2016-07-20 河南牧业经济学院 Traditional Chinese medicine microecological preparation for improving laying hen performance and preparation method of traditional Chinese medicine microecological preparation for improving laying hen performance

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101401921A (en) * 2008-10-30 2009-04-08 河南省龙腾高科实业有限公司 Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof
CN102232474A (en) * 2011-04-15 2011-11-09 北京康华远景科技有限公司 Disease-resistant growth promoter for aquatic animals and preparation method thereof
CN105767587A (en) * 2016-04-29 2016-07-20 河南牧业经济学院 Traditional Chinese medicine microecological preparation for improving laying hen performance and preparation method of traditional Chinese medicine microecological preparation for improving laying hen performance

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
博胜才等: "《新编兽药手册》", 30 April 2011, 湖南科学技术出版社 *
荆所义等: "《中药防治畜禽疾病实用技术》", 31 August 2011, 中原农民出版社 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107467404A (en) * 2017-10-23 2017-12-15 潍坊华光数码设备有限公司 A kind of feed addictive for laying hen health care and preparation method thereof
CN110897053A (en) * 2019-12-20 2020-03-24 姜丹 Fly-repelling traditional Chinese medicine fermented feed for improving egg quality of laying hens and preparation method and application thereof
CN110897053B (en) * 2019-12-20 2023-05-05 沈阳丰美生物技术有限公司 Fly-expelling traditional Chinese medicine fermented feed for improving egg quality of laying hens and preparation method and application thereof

Similar Documents

Publication Publication Date Title
CN102178108B (en) Chinese medicinal herb feed additive for promoting growth and weight gain of mutton sheep
CN102078512B (en) Pharmaceutical composition for improving fatigue and preparation method thereof
CN106619941A (en) Traditional Chinese medicine fermented preparation for improving production performance of sows, preparation method and application thereof
CN107006708A (en) Piglet Compound Chinese Herbs for Fatigue-Resistance of Animal, its preparation method and application prepared by a kind of asynchronous fermentation method
CN102028118A (en) Chinese herbal additive for producing non-polluted eggs and preparation method thereof
CN101554238A (en) Mind-tranquilizing brain-strengthening egg and preparing method thereof
CN102232548A (en) Cordyceps militaris-American ginseng capsule health-care product and preparation process thereof
CN106729108A (en) A kind of herb fermenting preparation of antiviral and enhance immunity and preparation method thereof
CN101869287B (en) Astragalus and honey soft capsule and preparation method thereof
CN105028930B (en) A kind of Chinese herbal feed reducing egg cholesterol
CN107279984A (en) Technology of preparing with the gentle physical fatigue functional health product of strengthen immunity
CN106924477A (en) A kind of compound Chinese medicine fermentation preparation of mixed fermentation production and preparation method thereof
CN107823544A (en) A kind of herb fermenting preparation for improving ruminant digestion performance and preparation method thereof
CN104784505A (en) Dendrobium officinale healthcare product with auxiliary blood glucose reducing function and preparation method thereof
CN104839426B (en) A kind of biological fermentation feed and preparation method thereof
CN106994157A (en) A kind of herb fermenting preparation, its preparation method and application for improving performance in layers
CN105533255A (en) Application of Chinese herbal medicine feed additive
CN103920140B (en) A kind of people is with blood sugar lowering Weight-reducing and lipid-lowering compound preparation
CN106562091A (en) Compound plant feed additive for laying fowls and preparation method thereof
CN106822522A (en) A kind of preparation method and application for improving poultry growth performance and immunity herb fermenting preparation
CN100360179C (en) Medicine for treating infantile anorexia
CN101336705B (en) Health food and its preparation method
CN108379548A (en) It is a kind of have relieve fatigue drug or health-care food composition of effect and preparation method thereof
CN105558350A (en) Feed containing Chinese herbal medicine compound crude polysaccharide and preparation method of feed
CN105535282A (en) Biological agent for irritable infertility function repair and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20170801