CN106729108A - A kind of herb fermenting preparation of antiviral and enhance immunity and preparation method thereof - Google Patents
A kind of herb fermenting preparation of antiviral and enhance immunity and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/346—Platycodon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/804—Rehmannia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/808—Scrophularia (figwort)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8964—Anemarrhena
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
Abstract
Veterinary Chinese medicine fermentation preparation the present invention relates to a kind of antiviral and enhance immunity and preparation method thereof, the parts by weight of its each component are：5 parts of the Radix Astragali, 5 parts of lophatherum gracile, 3 parts of Radix Glycyrrhizae, 5 parts of balloonflower root, 6 parts of the capsule of weeping forsythia, 6 parts of the wind-weed, 5 parts of radix scrophulariae, 5 parts of the radix paeoniae rubrathe, 4 parts of the root bark of tree peony, 6 parts of cape jasmine, 13 parts of cornu bubali, 6 parts of radix rehmanniae recen, 26 parts of gypsum.The inventive method choose traditional Chinese medicinal components are rational in infrastructure, ratio is appropriate, the advantages such as the active height of Lactobacillus plantarum, the strong stress resistance of selection, in the presence of the Lactobacillus plantarum selected by the present invention, the drug effect of original medicine is significantly improve, domestic animal immunity of organism level can be significantly improved using fermentation medicine of the invention.
Description
Technical field
The present invention relates to veterinary formulations technical field, more particularly to a kind of herb fermenting preparation, Preparation Method And The Use.
Background technology
The disease of puzzlement pig industry is a lot of at present, and common multiple not lower 70 kinds both at home and abroad, domestic Prevention of Common Occurrence Porcine Disease toxicity is passed
Catch an illness about 16 kinds：The economic loss that wherein swine fever, pseudoabies, blue otopathy, smoothing filter operator, five kinds of diseases of aftosa are brought is huge
Greatly, strong, the popular wide, death rate of its infectiousness is high, as new " five big infectious diseases ".The fast development of modern pharmaceutical technology,
The development of pig industry is greatly promoted, wherein antibiotic, chemical synthetic drug is keeping swinery health and promoting the aspects such as growth
Important function has been played, but the drug resistance that excessively can cause pathogenic bacteria using antibiotic in animal husbandry strengthens and medicament residue
The problems such as, significant damage is brought to animal husbandry and human health.
The content of the invention
For problems of the prior art, it is an object of the invention to provide a kind of natural safe herb fermenting preparation
And the method for preparing the fermentation preparation, while, there is provided it is a kind of to exempt from antiviral and raising containing the herb fermenting preparation
The feed of epidemic disease power.
In order to solve the above-mentioned technical problem, the present invention is achieved using following technical scheme：
A kind of Chinese medicine composition for improving domestic animal immunity, is prepared from by following components according to parts by weight：The Radix Astragali 5~
10 parts, 5~10 parts of lophatherum gracile, 3~5 parts of Radix Glycyrrhizae, 5~10 parts of balloonflower root, 5~10 parts of the capsule of weeping forsythia, 5~10 parts of the wind-weed, radix scrophulariae 5~10
Part, 5~10 parts of the radix paeoniae rubrathe, 1~5 part of the root bark of tree peony, 5~10 parts of cape jasmine, 10~15 parts of cornu bubali, 5~10 parts of radix rehmanniae recen, gypsum 20~
30 parts.The method that preparation method uses ultramicro grinding, by ultramicro grinding after each component mixing in composition to 700 mesh.
A kind of herb fermenting preparation, it mixed with Lactobacillus plantarum bacterium solution by traditional Chinese medicine ingredients after through fermentation be made, it is described in
Medicine composition is by weight：5~10 parts of the Radix Astragali, 5~10 parts of lophatherum gracile, 3~5 parts of Radix Glycyrrhizae, 5~10 parts of balloonflower root, company
Stick up 5~10 parts, 5~10 parts of the wind-weed, 5~10 parts of radix scrophulariae, 5~10 parts of the radix paeoniae rubrathe, 1~5 part of the root bark of tree peony, 5~10 parts of cape jasmine, cornu bubali 10
~15 parts, 5~10 parts of radix rehmanniae recen, 20~30 parts of gypsum.
On the basis of such scheme, the traditional Chinese medicine ingredients are by weight：5 parts of the Radix Astragali, 5 parts of lophatherum gracile,
3 parts of Radix Glycyrrhizae, 5 parts of balloonflower root, 6 parts of the capsule of weeping forsythia, 6 parts of the wind-weed, 5 parts of radix scrophulariae, 5 parts of the radix paeoniae rubrathe, 4 parts of the root bark of tree peony, 6 parts of cape jasmine, 13 parts of cornu bubali, life
6 parts of glutinous rehmannia, 26 parts of gypsum.
On the basis of such scheme, Lactobacillus plantarum content >=10 in the bacterium solution8CFU/ml。
The preparation method of above-mentioned Chinese medicine preparation, comprises the following steps：
(1) by traditional Chinese medicinal components are measured, crush, cross 60 eye mesh screens, it is standby；
(2) each component after crushing is well mixed, adds the water of traditional Chinese medicine ingredients gross weight 80%, stir after
100 DEG C of steam sterilizing 30min, are cooled to 20-30 DEG C；
(3) to bacterium solution is added in the medicine after cooling, bacterium solution consumption accounts for 10~15% of total amount after adding；It is placed in unidirectional row
In gas polybag, fermented 30 days under the conditions of 20 DEG C~30 DEG C.Above-mentioned one-way exhaust polybag is Patent No.：
The one-way exhaust bag of 200920100585.9 anaerobic fermentation feed, Chinese medicine and additive.
On the basis of such scheme, the bacterium solution is obtained by following methods：Lactobacillus plantarum strain is inoculated in activation
Culture medium carries out activation culture, and cultivation temperature is 37 DEG C, time 24h；The strain that will have been activated is inoculated in fermentation tank, control temperature
37 DEG C~43 DEG C of degree, time 24h~36h.
On the basis of such scheme, the activation medium and the fermentation used medium are：MRS culture mediums, into
It is divided into (g/L)：Peptone 10.0, meat extract 10.0, yeast extract 5.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, hydrogen citrate two
Ammonium 2.0, sodium acetate 5.0, magnesium sulfate 0.58, manganese sulfate 0.28, cysteine 0.5, Tween 80 1.0, adjust pH6.2-6.4.
It is a kind of with antiviral and enhance immunity livestock feed, the above method is particularly added in livestock feed
The Chinese medicine preparation of preparation, addition be feed per ton in add 2kg.
Antiviral, enhance immunity veterinary Chinese medicinal composition of the invention, the property of medicine drug effect of each Chinese medicine is as follows：
The Radix Astragali：For pulse family herbaceos perennial is mainly Astragalus membranacus, also there are the dry root of Inner Mongolia Astragalis, wild or cultivation
Training, containing main components such as alkaloid, folic acid, crystallinity neutral substance, choline, amino acid, with tonifying Qi and lifting yang, strengthening exterior and reducing sweat,
The effects such as inducing diuresis for removing edema, pus draining and toxin expelling.
Lophatherum gracile：The main components such as the alias leaf of bamboo, Arundoin, cogongrass terpene, property is sweet light cold, with work(such as antipyretic diuresis
Effect.
Radix Glycyrrhizae：Alias RADIX GLYCYRRHIZAE, is the dry root and root-like stock of pulse family herbaceos perennial Radix Glycyrrhizae and glycyrrhiza glabra etc.
(reed head), it is mostly wild, containing glycyrrhizin, liquiritigenin, glucose, mannitol, malic acid, asparagine etc. it is main into
Point, with invigorating the spleen and benefiting qi, it is clearing heat and detoxicating, the effects such as moisten the lung and relieve the cough.
Balloonflower root：It is the root of Campanulaceae balloonflower root ,-β-D-Glucose glucoside, Δ is transplanted containing saponin, spinasterol, α-spinach steroid
7- stigmastenol, betulin, and containing synanthrin, platycodonin, triterpene alkenes material：The main components such as platycogenic acid A, B and C, tool
There is out the effects such as facilitaing lung gas, eliminating the phlegm apocenosis.
The capsule of weeping forsythia：For the perennial machaka capsule of weeping forsythia of Oleaceae dry almost ripe fruit (blue or green shell) and maturation after shell
(sticking up always), wild or cultivation, containing forsythol, oleanolic acid, the main component such as a kind of sterol compound and citrin, with anti-
Bacterium is antiviral and the effects such as cardiac stimulant diuresis.
The wind-weed：Be the rhizome of the liliaceous plant wind-weed, mainly containing saponin component, with antibacterial it is antipyretic the effects such as.
Radix scrophulariae：Alias radix scrophulariae is Scrophulariaceae herbaceos perennial radix scrophulariae (Zhejiang radix scrophulariae) and its belongs to the drying of scrophularia buergeriana together
Root.Mostly cultivate, the main component such as glucoside containing the Black Warrior, phytosterol, linolenic acid, alkaloid, with nourishing Yin and falling fire, in vitro in
And the effects such as diphtheria toxin.
The radix paeoniae rubrathe：For Ranunculaceae herbaceos perennial Chinese herbaceous peony and with drafting a document Chinese herbaceous peony, the river radix paeoniae rubrathe, hair leaf Chinese herbaceous peony, Mao Yecao Chinese herbaceous peonies
The dry root of medicine, beautiful Chinese herbaceous peony etc., be it is wild, containing main components such as benzoic acid, tannin, Paeoniflorins, with sedation and analgesia, anti-
Bacterium is antiviral, the expansion heart coronary artery the effects such as.
The root bark of tree peony：Be the dry root skin of Ranunculaceae machaka tree peony, cultivation or it is wild, containing Paeonol (i.e. peonol),
Moutan bark original glucoside, benzoic acid, phytosterol, the main component such as tannin, the effects such as with antibacterial, step-down.
Cape jasmine：It is the dry mature fruit of Rubiaceae evergreen shrubs cape jasmine tree, how wild, a large amount of cultivations, containing cape jasmine
The main components such as glucoside, crocin, Gardenia Yellow, with antipyretic, cholagogic, hemostasis, antibacterial, calmness, step-down the effects such as.
Cornu bubali：The angle of bovid buffalo, it is main containing sterols, amino acids, peptides, guanidine derivative, protein etc.
Want composition, with heat-clearing, cool blood, removing toxic substances the effects such as.
Radix rehmanniae recen：The root tuber of scrophulariaceae rehmannia glutinosa plant, containing Catalpol, amino acids, sugar, mannitol, cupreol and rape oil
The main components such as sterol, with clearing heat and promoting fluid, hemostasis the effects such as.
Gypsum：It is mineral monoclinic system hydrous calcium sulfate ore, main component is hydrous calcium sulfate, is rushed down with heat-clearing
Fire, quench one's thirst relieving restlessness the effects such as.
Wherein described Bacillus acidi lactici is Lactobacillus plantarum plant subspecies
(Lactobacillusplantarumsubsp.Plantarum), numbering AS1.557, from the micro- life in Chinese Academy of Sciences Beijing
Thing research institute.
Know-why of the invention：
We are comprehensive《The Treatise on Fevrile Diseases》Baihu Tang,《Medical secrets of official》Draw《Essay side》Xijiao Dihuang Tang,《Wen Bing Tiao Bian》
The tripartites such as qingying decoction plus-minus is formed.Cornu bubali is reused in side and is equipped with the root bark of tree peony, the radix paeoniae rubrathe with removing pattogenic heat from the blood and toxic material from the body stagnation resolvation；Gypsum is reused, with cold
Stomach fire is rushed down in victory heat, clearing lung-heat；The wind-weed, the dried rhizome of rehmannia, radix scrophulariae kidney nourishing water, tonifying yin liquid；The leaf of bamboo, the capsule of weeping forsythia, cape jasmine all can in it is thorough in the heart, it is outer it is thorough in
Table, clear away heart-fire diuresis, and heat conduction is descending；Radix Glycyrrhizae coordinating the drug actions of a prescription, invigoration spleen qi, all medicines of the above are used in combination, among heat-clearing and with yin-nourishing, cool blood
Among and with dissipate the stasis of blood.In addition, balloonflower root eliminating the phlegm apocenosis in side, relieving the five internal organs, fill blood, with the Radix Astragali with strengthening spleen and middle warmer, benefit defends solid table, increases
Strong body's immunity.All medicines share, plays altogether：Strengthen the body resistance to consolidate the constitution, clearing heat and detoxicating, protecting liver and kidney, spleen benefiting and stimulating the appetite the effect of.
Microorganism has the ability of very powerful decomposition and inversion material, and can produce abundant secondary metabolite, leads to
The growth metabolism and vital movement of microorganism is crossed to process Chinese medicine, can be than general processing means physically or chemically more significantly
Degree ground changes the property of medicine, improves curative effect, reduces toxic and side effect, expands indication.
Herb fermenting preparation of the present invention has the characteristics that：(1) present invention is using during Lactobacillus plantarum growth metabolism
The enzyme of generation carries out the chemical reaction of structural modification to specific substrates, and compared with chemical reaction, it has region and three-dimensional selection
Property the advantages of strong, reaction condition is gentle, simple to operate, cost is relatively low, public hazards are few, and some chemical methodes can be completed be difficult to
Reaction；(2) Lactobacillus plantarum of the present invention can extracellular proteinase, cellulase, hemicellulase, pectin in metabolic process
Tens kinds of ectoenzymes such as enzyme, amylase enter culture medium, and what these enzymes had can form new change by drug ingedient decomposition and inversion
Compound, what is had can decompose the compact texture such as cellulose, hemicellulose, pectic substance, lignin in plant cell wall, promote medicine
Active component discharges to greatest extent；(3) present invention is selected Lactobacillus plantarum and its metabolite effectively supplement or enhancing
The function of original medicine；(4) Chinese medicine generates new active material after being converted through Lactobacillus plantarum, and brings new health care, pre-
Prevent or control treatment functions.
The inventive method choose traditional Chinese medicinal components are rational in infrastructure, ratio appropriate, the Lactobacillus plantarum of selection it is active it is high,
The advantages such as strong stress resistance, in the presence of the Lactobacillus plantarum selected by the present invention, significantly improve the drug effect of original medicine,
Domestic animal immunity of organism level can be significantly improved using fermentation medicine of the invention.
Specific embodiment
Below in conjunction with preferred embodiment, to the specific embodiment according to present invention offer, details are as follows：
Embodiment 1
The preparation of bacterium solution：
Lactobacillus plantarum strain of the invention is inoculated in into activation medium to be cultivated, cultivation temperature is 37 DEG C, time
24h；The strain that will have been activated is inoculated in fermentation tank, controls 37 DEG C~43 DEG C of temperature, and time 24h~36h prepares zymotic fluid.
The activation medium is：MRS culture mediums, composition is (g/L)：Peptone 10.0, meat extract 10.0, yeast extract
5.0th, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0, magnesium sulfate 0.58, manganese sulfate 0.28,
Cysteine 0.5, Tween 80 1.0, adjust pH6.2-6.4.
Fermentation used medium be：MRS culture mediums, composition is ibid.
After the completion of fermentation, bacterial content >=10 in bacterium solution8CFU/ml。
Embodiment 2
A kind of antiviral, enhance immunity veterinary Chinese medicine fermentation preparation, the parts by weight of its each component are：5 parts of the Radix Astragali,
5 parts of lophatherum gracile, 3 parts of Radix Glycyrrhizae, 5 parts of balloonflower root, 6 parts of the capsule of weeping forsythia, 6 parts of the wind-weed, 5 parts of radix scrophulariae, 5 parts of the radix paeoniae rubrathe, 4 parts of the root bark of tree peony, 6 parts of cape jasmine, water
13 parts of ox horn, 6 parts of radix rehmanniae recen, 26 parts of gypsum；
Specific preparation method is as follows：
(1) by traditional Chinese medicinal components are measured, crush, cross 60 eye mesh screens, it is standby；
(2) each component after crushing is well mixed, adds the water of traditional Chinese medicine ingredients gross weight 80%, stir after
100 DEG C of steam sterilizing 30min, are cooled to 20-30 DEG C；
(3) to bacterium solution is added in the medicine after cooling, bacterium solution consumption accounts for 10~15% of total amount after adding；It is placed in unidirectional row
In gas polybag, fermented 30 days under the conditions of 20 DEG C~30 DEG C.
Embodiment 3
A kind of antiviral, enhance immunity veterinary Chinese medicine fermentation preparation, the Chinese medicine be 10 parts of the Radix Astragali, 5 parts of lophatherum gracile,
3 parts of Radix Glycyrrhizae, 5 parts of balloonflower root, 5 parts of the capsule of weeping forsythia, 5 parts of the wind-weed, 5 parts of radix scrophulariae, 5 parts of the radix paeoniae rubrathe, 1 part of the root bark of tree peony, 5 parts of cape jasmine, 10 parts of cornu bubali, life
5 parts of glutinous rehmannia, 20 parts of gypsum.Preparation method is same as Example 2.
Embodiment 4
A kind of antiviral, enhance immunity veterinary Chinese medicine fermentation preparation, the Chinese medicine is 10 parts of the Radix Astragali, lophatherum gracile 10
Part, 5 parts of Radix Glycyrrhizae, 10 parts of balloonflower root, 10 parts of the capsule of weeping forsythia, 10 parts of the wind-weed, 10 parts of radix scrophulariae, 10 parts of the radix paeoniae rubrathe, 5 parts of the root bark of tree peony, 10 parts of cape jasmine, buffalo
15 parts of angle, 10 parts of radix rehmanniae recen, 30 parts of gypsum.Preparation method is same as Example 2.
The test of pesticide effectiveness：
1 materials and methods
1.1 experimental animals are close with daily ration selection parity, litter size stabilization (12-15) sow, from farrowed pig
Take the healthy three way cross piglet 150 of body weight close (there was no significant difference).Basal diet is according to pig nutrition requirements
Prepare, each test group adds each experiment prescription in basal diet, specific as follows：Ultramicro-powder is from this wherein used by Ultramicro-powder group
Chinese medicine composition in inventive embodiments 2 carries out the Ultramicro-powder that ultramicro grinding is obtained, and feeding method is：Ultramicro-powder group is in nursing
2kg Ultramicro-powders are added in feed, it is each that 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment add 2kg in the feed fed respectively
Fermentation preparation prepared by embodiment, blank control group is without medicine.
1.2 experimental designs and feeding and management
Experiment is divided into five groups, i.e. blank control group and four test groups, point three phases feeding, 30 days feeding piglet phases,
30 days middle swine rearing phases are fattened, it is fattening period to fatten middle pig to delivering for sale.The temperature and humidity raised in pig house is suitable, free choice feeding
And free water, remaining is carried out according to the normal feeding and management of plant and immune programme for children.
1.3 testing indexs
Sample collection：After each stage of feeding experiment terminates, blood sampling separates serum, and -20 DEG C of preservations are to be measured.
Index determining：Proleulzin, interleukin-6, CD-4, CD-8, γ-INF.Surveyed to specifications with ELIASA
Fixed, diagnostic kit is bought from the enzyme-linked bio tech ltd in Shanghai.
1.4 statistical analyses
All gathered datas carry out early stage treatment using Excel softwares, then carry out statistical analysis, LSD with SPSS 19.0
Multiple range test is carried out, is as a result represented using mean+SD.
2 results and analysis
Influence result of the 2.1 each experiment prescriptions to piglet immune factors in serum is as shown in table 1
Respectively influence of the experiment prescription to piglet immune factors in serum of table 1
| Index | Control group | Ultramicro-powder | 2 groups of embodiment | 3 groups of embodiment | 4 groups of embodiment |
| CD4 (%) | 22.05±0.31b | 23.91±0.81ab | 25.41±0.74a | 24.95±0.85a | 24.77±0.72a |
| CD8 (%) | 24.71±0.62a | 19.96±0.53ab | 16.80±0.35b | 17.31±0.49b | 17.56±0.46b |
| IL-2(pg/ml) | 391.6±6.01C | 550.1±8.14B | 791.3±7.55A | 778.7±8.02A | 786.3±7.90A |
| IL-6(pg/ml) | 852.3±11.81Bc | 951.5±8.44ab | 1120.7±13.95Aa | 1116.8±15.07Aa | 1110.6±17.12Aa |
| γ-INF(ng/L) | 858.1±9.01b | 935.7±4.20ab | 980.6±9.06a | 977.3±7.11a | 974.8±8.46a |
Note：Colleague's shoulder marking-up parent phase represents difference not significantly (P with or without letter>0.05), shoulder marking-up female difference small letter
Matrix shows significant difference (P<0.05), the female difference capitalization of shoulder marking-up represents difference extremely significantly (P<0.01).
For change of serum C D4 contents, test group is improved compared with control group, and wherein 2,3,4 groups of contents of embodiment are notable respectively
Improve 15.24%, 13.15% and 12.34% (P<0.05), Ultramicro-powder group improves 8.44% (P>0.05), wherein implementing
2 groups of highests of example, 1.84% and 2.58% (P has been respectively increased than 4 groups of 3 groups of embodiment and embodiment>0.05).Change of serum C D8 is contained
For amount, test group is below control group, and wherein 2,3,4 groups of embodiment significantly reduces 32.01%, 29.95% and respectively
28.94% (P<0.05), 19.22% (P of Ultramicro-powder group reduction>0.05).
Added in diet as can be seen from Table 1 it is each experiment prescription after piglet immune factors in serum IL-2, IL-6 and γ-
INF contents are improved compared with control group.For IL-2 contents, Ultramicro-powder group, 2,3 and 4 groups of embodiment extremely show respectively compared with control group
Work improves 158.5pg/ml, 399.7pg/ml, 387.1pg/ml and 394.7pg/ml (P<0.01)；2,3 and 4 groups of ratios of embodiment
The other pole of ultra micro powder component significantly improves 241.2pg/ml, 228.6pg/ml and 236.2pg/ml (P<0.01), embodiment 2,3 and 4
Not notable (the P of group difference>0.05).For IL-6 contents, Ultramicro-powder group, 2,3 and 4 groups of embodiment compared with control group respectively significantly,
Pole significantly improves 99.2pg/ml (P<0.05)、268.4pg/ml、264.5pg/ml、258.3pg/ml(P<0.01)；Embodiment
2nd, 3 and 4 groups do not significantly improve 169.2pg/ml, 165.3pg/ml and 159.1pg/ml (P than ultra micro powder component<0.05), and
Not notable (the P of 2,3 and 4 groups of differences of embodiment>0.05).For γ-INF contents, Ultramicro-powder group, 2,3 and 4 groups of ratios of embodiment
Control group is respectively increased 77.6ng/ml (P>0.05), 122.5ng/ml, 119.2ng/ml and 116.7ng/ml (P<0.05)；It is real
Apply 2,3 and 4 groups of example and improve 44.9ng/ml, 41.6ng/ml and 39.1ng/ml (P than Ultramicro-powder group respectively>0.05).
Influence of the 2.2 each experiment prescriptions to fattening middle Swine serum immune factor is as shown in table 2
Table 2 respectively tests prescription to fattening the influence of middle Swine serum immune factor
| Index | Control group | Ultramicro-powder | 2 groups of embodiment | 3 groups of embodiment | 4 groups of embodiment |
| CD4 (%) | 22.90±2.49bc | 27.33±1.96b | 34.00±2.41a | 33.33±2.35a | 30.90±2.28a |
| CD8 (%) | 23.45±1.08Aa | 22.06±0.74Aa | 13.52±0.19Bc | 18.95±0.15b | 19.08±0.21b |
| IL-2(pg/ml) | 580.9±15.44C | 760.4±11.83B | 995.0±10.78A | 920.7±14.31A | 914.7±12.64A |
| IL-6(pg/ml) | 670.1±19.88c | 813.5±35.75b | 1091.0±23.55a | 1067.5±26.88a | 1048.2±27.04a |
| γ-INF(ng/L) | 629.9±6.49b | 715.3±16.02ab | 819.2±12.11a | 810.8±10.38a | 805.1±15.22a |
Note：Colleague's shoulder marking-up parent phase represents difference not significantly (P with or without letter>0.05), shoulder marking-up female difference small letter
Matrix shows significant difference (P<0.05), the female difference capitalization of shoulder marking-up represents difference extremely significantly (P<0.01).
For change of serum C D4 contents, test group is improved compared with control group, and wherein 2,3,4 groups of contents of embodiment are notable respectively
Improve 48.47%, 45.54% and 34.93% (P<0.05), Ultramicro-powder group improves 19.34% (P>0.05), wherein implementing
2 groups of highests of example, 2.01% and 10.03% (P has been respectively increased than 4 groups of 3 groups of embodiment and embodiment>0.05).Change of serum C D8 is contained
For amount, test group is below control group, and wherein 2,3,4 groups of embodiment significantly reduces 42.34% (P respectively<0.01)、
19.19% and 18.64% (P<0.05), Ultramicro-powder group reduces 5.93% (P>0.05).
Added in diet as can be seen from Table 2 it is each experiment prescription after fatten middle Swine serum immune factor IL-2, IL-6 and
γ-INF contents are improved compared with control group.For IL-2 contents, Ultramicro-powder group, 2,3 and 4 groups of embodiment are extremely notable compared with control group
Improve 179.5pg/ml, 414.1pg/ml, 339.8pg/ml and 333.8pg/ml (P<0.01)；2,3 and 4 groups of embodiment is than super
Pole significantly improves 234.6pg/ml, 160.3pg/ml and 154.3 (P to micro mist group respectively<0.01), 2,3 and 4 groups of differences of embodiment
Not notable (P>0.05).For IL-6 contents, Ultramicro-powder group, 2,3 and 4 groups of embodiment are significantly improved compared with control group
143.4pg/ml、420.9pg/ml、397.4pg/ml、378.1pg/ml(P<0.05)；2,3 and 4 groups of embodiment is than Ultramicro-powder group
277.5pg/ml, 254.0pg/ml and 234.7pg/ml (P are significantly improved respectively<0.05), 2,3 and 4 groups of differences of embodiment
Not notable (P>0.05).For γ-INF contents, Ultramicro-powder group, 2,3 and 4 groups of embodiment are respectively increased than control group
85.4ng/ml(P>0.05), 189.3ng/ml, 180.9ng/ml and 175.2ng/ml (P<0.05)；Embodiment 2,3 and 4 components
103.9ng/ml, 95.5ng/ml and 89.8ng/ml (P are not improved than Ultramicro-powder group>0.05).
Influence of the 2.3 each experiment prescriptions to growing and fattening pigs immune factors in serum is shown in Table 3
Respectively influence of the experiment prescription to growing and fattening pigs immune factors in serum of table 3
| Index | Control group | Ultramicro-powder | 2 groups of embodiment | 3 groups of embodiment | 4 groups of embodiment |
| CD4 (%) | 15.85±0.42Cb | 20.72±0.33Ba | 43.21±0.84A | 42.84±2.35A | 42.53±2.28A |
| CD8 (%) | 18.30±0.36a | 15.52±0.24b | 12.48±0.19c | 12.65±0.15c | 12.71±0.21c |
| IL-2(pg/ml) | 583.4±13.05b | 631.2±12.61b | 730.1±11.57a | 721.4±13.62a | 715.2±15.22a |
| IL-6(pg/ml) | 1168.0±13.26a | 1193.7±16.49a | 1261.1±16.18a | 1224.3±18.25a | 1203.9±19.04a |
| γ-INF(ng/L) | 1095.9±25.91a | 1129.1±23.30a | 1190.3±19.04a | 1183.0±17.31a | 1184.7±20.18a |
Note：Colleague's shoulder marking-up parent phase represents difference not significantly (P with or without letter>0.05), shoulder marking-up female difference small letter
Matrix shows significant difference (P<0.05), the female difference capitalization of shoulder marking-up represents difference extremely significantly (P<0.01).
For change of serum C D4 contents, test group is significantly improved compared with control group pole, and Ultramicro-powder group, 2,3,4 groups of embodiment contain
Pole significantly improves 30.72%, 172.62%, 170.28% and 168.33% (P to amount respectively<0.01), wherein 2 groups of embodiment is most
Height, 108.54% (P has been respectively increased than 4 groups of Ultramicro-powder group, 3 groups of embodiment and embodiment<0.01), 0.86% and 1.60% (P
>0.05).For change of serum C D8 contents, test group is below control group, and Ultramicro-powder group, 2,3,4 groups of embodiment significantly drop respectively
Low 15.19%, 31.80%, 30.87% and 30.55% (P<0.05), 2,3 and 4 groups of embodiment is more notable than Ultramicro-powder group respectively
Reduce 19.59%, 18.49% and 18.10 (P<0.05).
Added in diet as can be seen from Table 3 it is each experiment prescription after growing and fattening pigs immune factors in serum IL-2, IL-6 and γ-
INF contents are improved compared with control group.For IL-2 contents, Ultramicro-powder group improves 47.8pg/ml (P compared with control group>0.05),
2,3 and 4 groups of embodiment significantly improves 146.7pg/ml, 138.0pg/ml and 131.8pg/ml (P compared with control group respectively<
0.05)；2,3 and 4 groups of embodiment does not significantly improve 98.9pg/ml, 90.2pg/ml and 84.0pg/ml (P than ultra micro powder component<
0.05), the not notable (P of 2,3 and 4 groups of differences of embodiment>0.05).For IL-6 contents, 2,3 and 4 groups of Ultramicro-powder group, embodiment
All increased compared with control group, but difference is not notable, be respectively increased 25.7pg/ml, 93.1pg/ml, 56.3pg/ml,
35.9pg/ml(P>0.05).For γ-INF contents, Ultramicro-powder group, 2,3 and 4 groups of embodiment are not raised than control component
33.2ng/ml, 94.4ng/ml, 87.1ng/ml and 88.8ng/ml (P>0.05)；2,3 and 4 groups of embodiment is respectively than Ultramicro-powder group
It has been respectively increased 61.2ng/ml, 53.9ng/ml and 55.6ng/ml (P>0.05).
This experimental study shows that to the herb fermenting agent for adding present invention preparation in growing-finishing pig diet blood can be improved
Clear middle CD4 levels simultaneously reduce change of serum C D8 levels, and the notable or pole level of signifiance has especially been reached at later stages, improve growth
The immunity of organism level of growing and fattening pigs, and as the increase of use time becomes apparent to improving immunity of organism level, increased machine
The anti-virus ability of body.
Pharmacological toxicity is tested
Acute toxicity test data
Detection reagent：Serum AST, ALT detection kits build up Bioengineering Research Institute purchased from Nanjing.
Testing equipment
Micro-200 semiautomatic biochemistry detectors, assay balance, disscting instrument, syringe, test tube etc..
Test method
It is the ICR mouse 100 of 18-22g to choose body weight, and after being raised through the adaptability of 3 days, 5 groups are divided at random, and (male and female are each
Half), Ultramicro-powder group, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment and blank control group, every group each 20；Blank control group
Gavage physiological saline, Ultramicro-powder used by Ultramicro-powder group carries out ultramicro grinding and obtains from the Chinese medicine composition in the embodiment of the present invention 2
The Ultramicro-powder for arriving；Ultramicro-powder group is gavaged Ultramicro-powder is soluble in water, and 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment respectively will be each
The fermentation preparation of embodiment preparation is soluble in water to be gavaged, Ultramicro-powder group, 2 groups of embodiment, 3 groups of embodiment, the medicine of 4 groups of embodiment
Addition is 0.1g/ml, 0.4ml/ times, three times a day, continuous 7 days.
Testing index and method
(1) clinicing symptom observation：The state of mind, the diet situation of daily viewing test mouse, dead feelings of being fallen ill per day entry
Condition.
(2) pathological observation：Cut open inspection observation internal organ are carried out for dead mouse to change.
(3) growth indexes observation：Routine weighing is carried out to experiment mouse；Off-test, slaughter mouse weigh in, internal organs weight it is dirty
Device assessment of indices：Cut open inspection rat, core dirty, spleen, liver, kidney, testis, ovary, weighs, and internal organs is calculated according to the following formula and is referred to
Number：Organ index=internal organs weight/body weight × 100.
(4) change in liver function observation：After off-test, mouse is slaughtered, take blood to separate serum, determine AST, ALT.
Data process＆analysis
All gathered datas carry out early stage treatment using Excel softwares, then carry out statistical analysis, LSD with SPSS 19.0
Multiple range test is carried out, is as a result represented using mean+SD.
Clinicing symptom observation
Control group and test group mouse, the state of mind and diet desire, to external world respond have no obvious exception.Each group mouse
Morbidity and death condition such as table 4 below.
Table 4
| Packet | Sum | Dead (only) | The death rate (%) |
| Ultramicro-powder | 20 | 0 | 0 |
| 2 groups of embodiment | 20 | 0 | 0 |
| 3 groups of embodiment | 20 | 0 | 0 |
| 4 groups of embodiment | 20 | 0 | 0 |
| Control group | 20 | 0 | 0 |
Pathological observation：Control group and test group, mouse carried out after slaughtering cut open inspection observe have no observation internal organs it is obvious
Pathological change.
Growth indexes are observed：Off-test, slaughters that mouse weighs in, internal organs weight analysis are shown in Table 5.
Table 5
| Packet | Liver index | Cardiac index | Renal index | Lungs index | Index and spleen index |
| Ultramicro-powder | 54.19±1.75a | 4.80±0.29a | 13.61±0.19a | 6.73±0.60a | 5.12±0.55b |
| 2 groups of embodiment | 54.31±1.69a | 4.66±0.22a | 13.58±0.24a | 6.84±0.47a | 5.50±0.38b |
| 3 groups of embodiment | 53.83±1.81a | 4.71±0.28a | 13.32±0.25a | 6.49±0.55a | 5.39±0.49b |
| 4 groups of embodiment | 54.02±1.74a | 4.39±0.20a | 13.21±0.22a | 6.73±0.35a | 5.45±0.36b |
| Control group | 53.91±1.85a | 4.57±0.28a | 13.39±0.21a | 6.72±0.42a | 4.01±0.43a |
As shown in Table 5, test group significantly increases the index and spleen index of ICR mouse, 2,3 and 4 groups of differences of Ultramicro-powder group and embodiment
It is different not notable；There was no significant difference with test group for other index control groups.
Change in liver function is observed
After off-test, mouse is slaughtered, take blood to separate serum, determine AST (glutamic-oxalacetic transaminease), ALT (third turn of ammonia of paddy
Enzyme) it is shown in Table 6.Result display each group of data difference compared with blank control group is not notable, all in normal range (NR).
Table 6
| Packet | Sum | ALT | AST |
| Ultramicro-powder | 20 | 38.92±2.29a | 105.24±4.43a |
| 2 groups | 20 | 39.29±2.45a | 105.78±3.75a |
| 3 groups | 20 | 39.18±2.81a | 105.19±4.03a |
| 4 groups | 20 | 39.34±2.65a | 104.92±8.55a |
| Control group | 20 | 39.25±2.48a | 105.27±9.23a |
Long term toxicity test data
According to fermentation preparation of the present invention prepared by embodiment 2,20g/kg, 100g/kg give to 1 monthly age ICR mouse continuous gavage
Medicine 1 month, the fur of animal, the colour of skin, ingest, activity, the blood such as blood routine, the Liver and kidney function of animal without exception such as urine, feces
The main organs index of biochemical indicator and animal shows in normal range (NR), and normal kinetic indifference histopathologic examination,
The important organs such as the heart, liver, spleen, lung, kidney, adrenal gland, tracheae, testis, ovary are without pathological change.It is each more than being checked after being discontinued two weeks
Item index is reacted without overt toxicity, is shown without cumulative toxicity.
The above, is only presently preferred embodiments of the present invention, is not the limitation for making other forms to the present invention, is appointed
What those skilled in the art changed possibly also with the technology contents of the disclosure above or be modified as equivalent variations etc.
Effect embodiment.But it is every without departing from technical solution of the present invention content, according to technical spirit of the invention to above example institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.
Claims (10)
1. it is a kind of improve domestic animal immunity Chinese medicine composition, it is characterised in that by following components according to parts by weight prepare and
Into：5~10 parts of the Radix Astragali, 5~10 parts of lophatherum gracile, 3~5 parts of Radix Glycyrrhizae, 5~10 parts of balloonflower root, 5~10 parts of the capsule of weeping forsythia, 5~10 parts of the wind-weed,
5~10 parts of radix scrophulariae, 5~10 parts of the radix paeoniae rubrathe, 1~5 part of the root bark of tree peony, 5~10 parts of cape jasmine, 10~15 parts of cornu bubali, 5~10 parts of radix rehmanniae recen,
20~30 parts of gypsum.
2. it is according to claim 1 improve domestic animal immunity Chinese medicine composition, it is characterised in that by following components according to
Parts by weight are prepared from：It is 5 parts of the Radix Astragali, 5 parts of lophatherum gracile, 3 parts of Radix Glycyrrhizae, 5 parts of balloonflower root, 6 parts of the capsule of weeping forsythia, 6 parts of the wind-weed, 5 parts of radix scrophulariae, red
5 parts of Chinese herbaceous peony, 4 parts of the root bark of tree peony, 6 parts of cape jasmine, 13 parts of cornu bubali, 6 parts of radix rehmanniae recen, 26 parts of gypsum.
3. a kind of herb fermenting preparation, it is characterised in that it makes after being mixed with Lactobacillus plantarum bacterium solution by traditional Chinese medicine ingredients through fermentation
Into the traditional Chinese medicine ingredients are by weight：5~10 parts of the Radix Astragali, 5~10 parts of lophatherum gracile, 3~5 parts of Radix Glycyrrhizae, balloonflower root 5
~10 parts, 5~10 parts of the capsule of weeping forsythia, 5~10 parts of the wind-weed, 5~10 parts of radix scrophulariae, 5~10 parts of the radix paeoniae rubrathe, 1~5 part of the root bark of tree peony, cape jasmine 5~10
Part, 10~15 parts of cornu bubali, 5~10 parts of radix rehmanniae recen, 20~30 parts of gypsum.
4. herb fermenting preparation according to claim 3, it is characterised in that the traditional Chinese medicine ingredients are by weight
For：5 parts of the Radix Astragali, 5 parts of lophatherum gracile, 3 parts of Radix Glycyrrhizae, 5 parts of balloonflower root, 6 parts of the capsule of weeping forsythia, 6 parts of the wind-weed, 5 parts of radix scrophulariae, 5 parts of the radix paeoniae rubrathe, 4 parts of the root bark of tree peony,
6 parts of cape jasmine, 13 parts of cornu bubali, 6 parts of radix rehmanniae recen, 26 parts of gypsum.
5. the herb fermenting preparation according to claim 3 or 4, it is characterised in that Lactobacillus plantarum content in the bacterium solution
≥108CFU/ml。
6. the preparation method of the Chinese medicine preparation according to claim any one of 3-5, it is characterised in that comprise the following steps：
(1) by traditional Chinese medicinal components are measured, crush, cross 60 eye mesh screens, it is standby；
(2) each component after crushing is well mixed, adds the water of traditional Chinese medicine ingredients gross weight 80%, stirred after 100 DEG C
Steam sterilizing 30min, is cooled to 20-30 DEG C；
(3) to bacterium solution is added in the medicine after cooling, bacterium solution consumption accounts for 10~15% of total amount after adding；It is placed in one-way exhaust modeling
In pocket, fermented 30 days under the conditions of 20 DEG C~30 DEG C.
7. the preparation method of Chinese medicine preparation according to claim 6, it is characterised in that the bacterium solution is by following methods system
：Lactobacillus plantarum strain is inoculated in into activation medium carries out activation culture, and cultivation temperature is 37 DEG C, time 24h；Will activation
Good strain is inoculated in fermentation tank, controls 37 DEG C~43 DEG C of temperature, time 24h~36h.
8. the preparation method of Chinese medicine preparation according to claim 7, it is characterised in that the activation medium and the hair
Ferment used medium is：MRS culture mediums, composition is (g/L)：Peptone 10.0, meat extract 10.0, yeast extract 5.0, glucose
20.0th, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0, magnesium sulfate 0.58, manganese sulfate 0.28, cysteine
0.5th, Tween 80 1.0, adjust pH 6.2-6.4.
9. the purposes of the herb fermenting preparation described in a kind of any one of claim 3-5, it is characterised in that antiviral for domestic animal
With raising domestic animal immunity.
10. a kind of with antiviral and enhance immunity livestock feed, it is characterised in that：Right is added in livestock feed will
Seek Chinese medicine preparation prepared by 6 method, addition is to add 2kg in feed per ton.
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| CN108143958A (en) * | 2018-01-19 | 2018-06-12 | 西北农林科技大学 | It is a kind of to be used to treat Chinese medicinal formulae of canine distemper virus and preparation method thereof |
| CN112022980A (en) * | 2020-10-30 | 2020-12-04 | 河南豫商生物科技集团有限公司 | Traditional Chinese medicine formula for preventing African swine fever virus and preparation process of traditional Chinese medicine formula |
| CN114569678A (en) * | 2020-11-18 | 2022-06-03 | 山东绿都安特动物药业有限公司 | Veterinary scourge-clearing toxin-vanquishing granules and preparation method thereof |
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Application publication date: 20170531 |