CN108124451B - 用于从组织样品提取dna和rna的组合物和方法 - Google Patents

用于从组织样品提取dna和rna的组合物和方法 Download PDF

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CN108124451B
CN108124451B CN201680055819.5A CN201680055819A CN108124451B CN 108124451 B CN108124451 B CN 108124451B CN 201680055819 A CN201680055819 A CN 201680055819A CN 108124451 B CN108124451 B CN 108124451B
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CN108124451A (zh
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K·E·侯
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Cepheid
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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CN201680055819.5A 2015-07-24 2016-07-12 用于从组织样品提取dna和rna的组合物和方法 Active CN108124451B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562196774P 2015-07-24 2015-07-24
US62/196,774 2015-07-24
PCT/US2016/041917 WO2017019293A1 (en) 2015-07-24 2016-07-12 Compositions and methods for dna and rna extraction from tissue samples

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CN108124451A CN108124451A (zh) 2018-06-05
CN108124451B true CN108124451B (zh) 2022-09-09

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US (1) US10465182B2 (enExample)
EP (2) EP3325647B1 (enExample)
JP (1) JP7099951B2 (enExample)
CN (1) CN108124451B (enExample)
AU (1) AU2016298048B2 (enExample)
CA (1) CA2993447C (enExample)
DK (1) DK3325647T3 (enExample)
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WO (1) WO2017019293A1 (enExample)

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EP3325647B1 (en) 2015-07-24 2020-02-19 Cepheid Compositions and methods for dna and rna extraction from tissue samples
CN112534237B (zh) * 2018-06-21 2024-12-10 基美健有限公司 分析前基底处理的系统和方法
CN109234269B (zh) * 2018-09-21 2021-10-12 深圳金普迈生物科技有限公司 从甲醛固定的微量样品中提取并扩增dna片段的方法
KR102041333B1 (ko) * 2018-12-24 2019-11-06 주식회사 덴오믹스 세포 용해 조성물 및 이를 이용한 핵산의 추출 방법
CN110835628B (zh) * 2019-11-25 2021-07-27 宁波艾捷康宁生物科技有限公司 一种石蜡切片基因组dna提取的除蜡裂解液及提取试剂盒和提取方法
CN111057704B (zh) * 2019-12-31 2022-07-05 广州市金圻睿生物科技有限责任公司 一种石蜡切片样本dna提取的脱蜡裂解结合液、试剂盒及方法
US20220298572A1 (en) 2021-03-16 2022-09-22 Cepheid Compositions and methods for discriminating bacterial and viral infections in a human subject
US20230140360A1 (en) 2021-10-22 2023-05-04 Cepheid Compositions and methods of diagnosing and treating tuberculosis
TWI771253B (zh) * 2021-12-27 2022-07-11 財團法人工業技術研究院 戶外用的簡易式核酸萃取套組以及於戶外萃取樣本的核酸的方法
CN117925598B (zh) * 2023-12-12 2025-12-09 阔然生物医药科技(上海)有限公司 一种新鲜脑组织的核酸快速抽提方法

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EP3705582B1 (en) 2025-09-03
EP3325647A1 (en) 2018-05-30
WO2017019293A1 (en) 2017-02-02
CA2993447C (en) 2024-01-02
CN108124451A (zh) 2018-06-05
US10465182B2 (en) 2019-11-05
DK3325647T3 (da) 2020-05-18
AU2016298048A1 (en) 2018-02-15
CA2993447A1 (en) 2017-02-02
EP3705582A1 (en) 2020-09-09
AU2016298048B2 (en) 2022-08-04
ES2795048T3 (es) 2020-11-20
US20170022493A1 (en) 2017-01-26
JP2018524016A (ja) 2018-08-30
EP3325647B1 (en) 2020-02-19
JP7099951B2 (ja) 2022-07-12

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