CN108094771A - 含有金枪鱼活性多肽复配物的制备方法 - Google Patents
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Abstract
本发明公开一种含有金枪鱼活性多肽复配物的制备方法，属于饮品制备技术领域，复配物的制备方法，包括金枪鱼活性多肽制备，复配物的制备，冷却及检验，复配物的制备步骤为：按重量份计取粉碎后的盐蒿粉末和纤维素酶，加入蒸馏水并调pH值，于60~64℃下酶解，过滤取酶解液，加入无水乙醇和抗氧化剂搅拌，加热干燥得浸膏，备用，将白糖、苹果酸、蜂蜜、金枪鱼活性多肽和葡萄汁溶解得基液，将浸膏加入水加热溶解并加入基液，得复配物，本发明制备的复配物具有独特的盐蒿风味，有益于咽喉、口腔和牙齿健康，具有保健和抗氧化功效。
Description
技术领域
本发明属于饮品制备技术领域，具体涉及含有金枪鱼活性多肽复配物的制备方法。
背景技术
饮料是人们重要的生活消费品，目前市场上，饮料数量和饮料种类丰富多彩，各具特色。然而，除包装饮用水之外，其他饮料，如碳酸饮料、果蔬汁饮料、茶饮料等，通常含糖量较高。研究表明，长期食用高含糖、尤其是高含蔗糖的食品、已成为诱发糖尿病、肥胖症等病症的病因。同时，由于蔗糖等糖类，粘度较高，并且容易被口腔内细菌利用、发酵，产生有害的酸性物质，从而导致龋齿、口腔异味、咽喉炎等病症，长期饮用这类饮料，不利于身体健康。
发明内容
本发明的目的在于提供一种制备的复配物具有独特的盐蒿风味，有益于咽喉、口腔和牙齿健康，具有保健和抗氧化功效的含有金枪鱼活性多肽复配物的制备方法。
本发明为实现上述目的所采取的技术方案为：含有金枪鱼活性多肽复配物的制备方法，包括金枪鱼活性多肽制备，复配物的制备，冷却及检验，复配物的制备步骤为：按重量份计取粉碎后的盐蒿粉末和纤维素酶，质量比为150~200:1，加入蒸馏水并调pH值至5.5~6.0，固液比为1g：10-20ml，于60~64℃下酶解30-35min，过滤取酶解液，加入无水乙醇和抗氧化剂搅拌20-25min，加热干燥得浸膏，备用，将白糖、苹果酸、蜂蜜、金枪鱼活性多肽和葡萄汁溶解得基液，将浸膏加入2倍体积的水加热溶解并加入浸膏质量20~35%的基液，得复配物，选用盐蒿为主原料使制备的复配物中的主要口感为盐蒿风味，且复配物具有很好的清热、消积、扩张血管、防止心脏病和增强人体免疫力的功效，即复配物具有很好的保健效果，在复配物制备过程中选用金枪鱼活性多肽、葡萄汁、蜂蜜、白糖和苹果酸协同发挥作用提高复配物的抗氧化、润喉、洁齿和口腔保健的效果，还可改善盐蒿味的复配物的口感和香味，掩盖复配物中的苦味使复配物的香味浓郁，口感酸甜可口，且复配物的制备方法简单，所用原料易得，可实现大规模生产，利用提高盐蒿和金枪鱼鱼骨的经济利用价值。
优选的，得到复配物后装罐于103-105℃条件下持续15-20s高温瞬时灭菌，利用高温瞬时灭菌最大限度的减少制备的复配物中的营养成分被破坏并快速杀死细菌，有效延长制备的复配物的保质期及保质效果。
优选的，复配物由以下成分及重量份组成：盐蒿粉末300-420份、纤维素酶1-3份、蒸馏水700-1000份、无水乙醇40-60份、抗氧化剂0.3-0.7份、白糖20-45份、苹果酸27-50份、蜂蜜30-60份、金枪鱼活性多肽2-5份、葡萄汁20-60份，本发明的复配物具有优异的清热、消积、扩张血管、防止心脏病、增强人体免疫力、抗氧化性、防衰老、抗肿瘤、润喉及改善口腔健康的效果，选用盐蒿为主原料使制备的复配物中的主要口感为盐蒿风味，并且复配物味道香醇浓郁，口感酸甜可口，抗氧化性能优越，含糖量低，不会诱发糖尿病、肥胖症等症状，适合儿童、中老年人饮用，具有很大的市场及经济价值。
优选的，抗氧化剂由3-5重量份的维生素C和0.2-0.4重量份的混合物组成，混合物为L-亮氨酰甘氨酸和D-亮氨酰甘氨酸，两者质量比为57~63：4~7，抗氧化剂可与O2 －·、HOO·及·OH快速反应，生成半脱氢抗坏血酸，清除单线态氧，还原硫自由基，还可使氧化的VE恢复成还原型，起到间接抗氧化的作用，并且通过加入的混合物对维生素C起到增效作用，混合物可与金属离子络合，钝化催化氧化作用，同时产生的氢离子可使抗氧化剂再生，实现永久性抗氧化的效果。
优选的，金枪鱼活性多肽的制备方法如下：取金枪鱼鱼骨，打碎，按固液比1:1~1.3加入蒸馏水于25~30℃条件下保温2-3h后分别用0.08~0.12mol/L的NaOH和0.4-0.5mol/L的EDTA-Na2溶液浸泡，过滤，得滤液A，加入滤液A质量0.3-0.4%的C6H11NO2，然后在滤液A中按固液比1:10-15加入0.4-0.45mol/L的乙酸溶液搅拌提取15-20h，于4000-5000r/min条件下离心10-12min，取上清液，上清液中加入NaCl至溶液最终浓度为2.5mol/L，同时析出絮状沉淀，于3-5℃条件下8000-10000r/min离心16-20min，得金枪鱼活性多肽，上述方法制得的金枪鱼活性多肽安全无毒，具有很好的抗氧化能力，可有效清除人体内的自由基或活性氧来预防因氧化而造成氧化过程中的机体损伤，通过在金枪鱼活性多肽制备过程中加入C6H11NO2可使金枪鱼活性多肽阻断脂质过氧化过程中自由基链式反应的作用进一步提高金枪鱼活性多肽预防因氧化而造成氧化过程中的机体损伤作用。
优选的，滤液A中加入乙酸溶液后用电棒搅拌，电棒两端电压为10-15V，搅拌速度为30-40r/min，采用带电压的搅拌棒进行搅拌使金枪鱼活性多肽结构中的芳香族氨基酸的酚腔基基团能够作为有效电子供体，提高制备的金枪鱼活性多肽中的疏水氨基酸含量，增强金枪鱼活性多肽的抗氧化效果。
优选的，复配物冷却保存温度为1-4℃，通过低温保存有效保证金枪鱼活性多肽的活性，利于活性多肽在人体肠胃中的吸收效果。
与现有技术相比，本发明的有益效果为：1）选用盐蒿为主原料使制备的复配物中的主要口感为盐蒿风味，复配物具有很好的清热、消积、扩张血管、防止心脏病和增强人体免疫力的功效；2）在复配物制备过程中选用金枪鱼活性多肽、葡萄汁、蜂蜜、白糖和苹果酸协同发挥作用提高复配物的抗氧化、润喉、洁齿和口腔保健的效果，还可改善盐蒿味的复配物的口感和香味，掩盖复配物中的苦味使复配物的香味浓郁，口感酸甜可口；3）复配物的制备方法简单，所用原料易得，可实现大规模生产，利用提高盐蒿和金枪鱼鱼骨的经济利用价值；4）复配物的抗氧化性能优越，含糖量低，不会诱发糖尿病、肥胖症等症状，适合儿童、中老年人饮用，具有很大的市场及经济价值。
附图说明
图1为实施例4中金枪鱼活性多肽溶液与相同浓度的Vc溶液比较结果图。
具体实施方式
以下结合实施例对本发明作进一步详细描述：
实施例1：
含有金枪鱼活性多肽复配物的制备方法，包括金枪鱼活性多肽，复配物的制备，冷却及检验，具体步骤如下：
金枪鱼活性多肽制备：取金枪鱼鱼骨，打碎，按固液比1:1加入蒸馏水于27℃条件下保温2h后分别用0.1mol/L的NaOH和0.42mol/L的EDTA-Na2溶液浸泡，过滤，得滤液A，加入滤液A质量0.34%的C6H11NO2，然后在滤液A中按固液比1:15加入0.44mol/L的乙酸溶液用电棒搅拌，电棒两端电压为10V，搅拌速度为30r/min，搅拌提取17h，于4500r/min条件下离心10min，取上清液，上清液中加入NaCl至溶液最终浓度为2.5mol/L，同时析出絮状沉淀，于3℃条件下9000r/min离心18min，得金枪鱼活性多肽；
复配物的制备：按重量份计取粉碎后的盐蒿粉末300份和纤维素酶2份，质量比为150:1，加入蒸馏水760份并调pH值至5.5，于62℃下酶解34min，过滤取酶解液，加入无水乙醇48份和抗氧化剂0.4份搅拌24min，加热干燥得浸膏，备用，将白糖28份、苹果酸34份、蜂蜜45份、金枪鱼活性多肽4份和葡萄汁30份溶解得基液，将浸膏加入2倍体积的水加热溶解并加入浸膏质量27%的基液，得复配物；抗氧化剂由3重量份的维生素C和0.25重量份的混合物组成，所述混合物为L-亮氨酰甘氨酸和D-亮氨酰甘氨酸，两者质量比为57：4；
高温瞬时灭菌：将复配物后装罐于105℃条件下持续18s高温瞬时灭菌；
冷却及检验：检查罐体有无破裂等异常现象，贴上标签后将复配物于3℃低温保存。
实施例2：
含有金枪鱼活性多肽复配物的制备方法，包括金枪鱼活性多肽，复配物的制备，冷却及检验，具体优选步骤如下：
金枪鱼活性多肽制备：取金枪鱼鱼骨，打碎，按固液比1:1.2加入蒸馏水于28℃条件下保温3h后分别用0.12mol/L的NaOH和0.45mol/L的EDTA-Na2溶液浸泡，过滤，得滤液A，加入滤液A质量0.34%的C6H11NO2，然后在滤液A中按固液比1:12加入0.42mol/L的乙酸溶液用电棒搅拌，电棒两端电压为10V，搅拌速度为30r/min，搅拌提取17h，搅拌提取18h，于4600r/min条件下离心10min，取上清液，上清液中加入NaCl至溶液最终浓度为2.5mol/L，同时析出絮状沉淀，于3℃条件下8000r/min离心18min，得金枪鱼活性多肽；
复配物的制备：按重量份计取粉碎后的盐蒿粉末400份和纤维素酶2份，质量比为200:1，加入蒸馏水800份并调pH值至6.0，于63℃下酶解33min，过滤取酶解液，加入无水乙醇50份和抗氧化剂0.5份搅拌24min，加热干燥得浸膏，备用，将白糖30份、苹果酸34份、蜂蜜36份、金枪鱼活性多肽4份和葡萄汁39份溶解得基液，将浸膏加入2倍体积的水加热溶解并加入浸膏质量22%的基液，得复配物；抗氧化剂由4重量份的维生素C和0.3重量份的混合物组成，混合物为L-亮氨酰甘氨酸和D-亮氨酰甘氨酸，两者质量比为63：5；
高温瞬时灭菌：将复配物后装罐于103℃条件下持续15s高温瞬时灭菌；
冷却及检验：检查罐体有无破裂等异常现象，贴上标签后将复配物于2℃低温保存。
实施例3：
含有金枪鱼活性多肽复配物的感官评定：
请10位产品质量检验所有一定感官评审经验的化验员对实施例1、实施例2和市售保健饮料对照组进行感官评定，评分标准见表1，每一指标均采用10分制，满分为50分，计算各指标得分后，乘以加权值后相加，即得感官评定分值。感官评定结果为：实施1为47.4、实施例2为49.1、对照组为43.2，由此可知，实施例1和实施例2的含有金枪鱼活性多肽复配物的感官评定得分高于对照组，更易于被消费者接受。
表1含有金枪鱼活性多肽复配物的感官评分标准
实施例4：
为考察制备的金枪鱼活性多肽的抗氧化能力，采取比较法进行测试，取浓度为9.30mg/mL的金枪鱼活性多肽溶液与相同浓度的Vc的抗氧化性进行比较，比较结果如图1所示。
本发明的操作步骤中的常规操作为本领域技术人员所熟知，在此不进行赘述。
以上所述的实施例对本发明的技术方案进行了详细说明，应理解的是以上所述仅为本发明的具体实施例，并不用于限制本发明，凡在本发明的原则范围内所做的任何修改、补充或类似方式替代等，均应包含在本发明的保护范围之内。
Claims (7)
1.含有金枪鱼活性多肽复配物的制备方法，包括金枪鱼活性多肽制备，复配物的制备，冷却及检验，其特征在于：所述复配物的制备步骤为：按重量份计取粉碎后的盐蒿粉末和纤维素酶，质量比为150~200:1，加入蒸馏水并调pH值至5.5~6.0，固液比为1g：10-20ml，于60~64℃下酶解30-35min，过滤取酶解液，加入无水乙醇和抗氧化剂搅拌20-25min，加热干燥得浸膏，备用，将白糖、苹果酸、蜂蜜、金枪鱼活性多肽和葡萄汁溶解得基液，将浸膏加入2倍体积的水加热溶解并加入浸膏质量20~35%的基液，得复配物。
2.根据权利要求1所述的含有金枪鱼活性多肽复配物的制备方法，其特征在于：所述得到复配物后装罐于103-105℃条件下持续15-20s高温瞬时灭菌。
3.根据权利要求1所述的含有金枪鱼活性多肽复配物的制备方法，其特征在于：所述复配物由以下成分及重量份组成：盐蒿粉末300-420份、纤维素酶1-3份、蒸馏水700-1000份、无水乙醇40-60份、抗氧化剂0.3-0.7份、白糖20-45份、苹果酸27-50份、蜂蜜30-60份、金枪鱼活性多肽2-5份、葡萄汁20-60份。
4.根据权利要求3所述的含有金枪鱼活性多肽复配物的制备方法，其特征在于：所述抗氧化剂由3-5重量份的维生素C和0.2-0.4重量份的混合物组成，所述混合物为L-亮氨酰甘氨酸和D-亮氨酰甘氨酸，两者质量比为57~63：4~7。
5.根据权利要求1所述的含有金枪鱼活性多肽复配物的制备方法，其特征在于：所述金枪鱼活性多肽的制备方法如下：取金枪鱼鱼骨，打碎，按固液比1:1~1.3加入蒸馏水于25~30℃条件下保温2-3h后分别用0.08~0.12mol/L的NaOH和0.4-0.5mol/L的EDTA-Na2溶液浸泡，过滤，得滤液A，加入滤液A质量0.3-0.4%的C6H11NO2，然后在滤液A中按固液比1:10-15加入0.4-0.45mol/L的乙酸溶液搅拌提取15-20h，于4000-5000r/min条件下离心10-12min，取上清液，上清液中加入NaCl至溶液最终浓度为2.5mol/L，同时析出絮状沉淀，于3-5℃条件下8000-10000r/min离心16-20min，得金枪鱼活性多肽。
6.根据权利要求5所述的含有金枪鱼活性多肽复配物的制备方法，其特征在于：所述滤液A中加入乙酸溶液后用电棒搅拌，所述电棒两端电压为10-15V，搅拌速度为30-40r/min。
7.根据权利要求1所述的含有金枪鱼活性多肽复配物的制备方法，其特征在于：复配物冷却保存温度为1-4℃。
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