CN108059528A - A kind of preparation method of drought resistance agent - Google Patents
A kind of preparation method of drought resistance agent Download PDFInfo
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- CN108059528A CN108059528A CN201711362992.2A CN201711362992A CN108059528A CN 108059528 A CN108059528 A CN 108059528A CN 201711362992 A CN201711362992 A CN 201711362992A CN 108059528 A CN108059528 A CN 108059528A
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention belongs to production technology of crops fields, and in particular to a kind of preparation method of drought resistance agent.The present invention prepares super strength water absorbent based on the sweet glycan of konjaku, and utilize acrylic acid, acrylamide monomer will be grafted on the main chain of the sweet glycan of konjaku, contain numerous polar groups on strand, hydrophily is very strong, in the environment of active water, ionization will occur, generate substantial amounts of charged ion, it is mutually exclusive due to similar charge, molecular skeleton just expands, hydrone is just held by storage in the gap of these expansions, so that Plant Drought Resistance improves, since super strength water absorbent can form hydrogel, so as to wrap up bentonite, after nutritional ingredient in bentonite first penetrates bentonite, Free water in hydrogel make nutritional ingredient start dissolving be discharged into again through gel layer in soil, promote crop root part growth.
Description
Technical field
The invention belongs to production technology of crops fields, and in particular to a kind of preparation method of drought resistance agent.
Background technology
China is a large agricultural country, while is also a country for water resource relative shortage.Since industrial water and city are used
Water constantly increases, and agricultural water is gradually less.In China's history, agricultural arid often results in very big social disaster.According to
Statistics for many years, in recent years the occurrence frequency of China's Droughts be continuously improved, Droughts area constantly expands, agriculture
Crops Drought disaster is also in increase trend into number.Especially under the overall background of Global climate change, arid is for agricultural production
Influence can further increase.
Arid is exactly when the water consumption of plant is more than the water deficit made during water suction in tissue, and excessive water deficit shows
As.Crop drought resistance refers to that crop endures arid and a kind of aggrieved property minimum, the underproduction is minimum, is crop in drought environment
Growth, breeding, existence and arid recover the ability of eubolism later.The drought resisting growth of crop has two approach at present：One
Kind is the suitable drought resisting high-yield crop kind of screening, another is using crop drought resistance cultivation technique.Direct-sowing dry rice be
On the basis of full drought site preparation, rice paddy seed is directly seeded in soil, seedling stage keeps ground moistening, and general rain supports plantation.Drought
Direct-seeding Rice using water wisely has the advantages such as drought-enduring, resistance to lean, the strong, wide adaptability of resistance, and significantly water-saving, energy saving, saving of labor.
But often there is percentage of seedgermination the in direct-sowing dry rice, seedling is not small and weak strong, and when plantation is susceptible to moisture unevenness, and sowing is deep
Degree is not suitable for, and applying excessive seed manure leads to problems such as local ammonia density excessively high.
Traditional water conservancy engineering efficiency is being improved, while improving farming measures, is increasing crop using drought resistance agent product
Drought-resistant ability under the conditions of equal aridic moisture, can substantially reduce production loss using the crop of drought resistance agent and be ground as new
Study carefully direction.
In existing drought resistance agent, Handilong is a kind of drought resistance agent using fulvic acid as main material, and drought resisting effect is good.
Have and inorganic calcium salt is used alone come keeping crop seedling from drought of soaking seed, but effect is unsatisfactory.Drought resistance agent technology at present more typically uses
High molecular polymer with strong absorptive soaks seed or is dressed seed for crop, but can only be acted as in crops seedling stage as water-retaining agent
With；Also have using antitranspirant, by foliage-spray, reagent is made to act on stomata, to reduce moisture loss, but drought resistance is not
Stablize, crop leaf transpiration is reduced during spraying, make the stomata closing of crop blade face, permeability reduces, influences to make
Object photosynthesis causes yield reduction that can also be impacted to leaf photosynthesis.In recent years, although occurring that seedling can be used for
Phase is handled, and can be used for the drought resistance agent type that Adult plant sprays, but this kind of drought resistance agent is mostly that a variety of chemical compositions compound,
Complicated component needs to be inquired into the security of crop, and application cost is higher；Meanwhile also have using plant growth regulator
The drought resistance agent that such as gibberellin is combined, but the residual of growth hormone, and result in the problem of new.
In summary, existing drought resistance agent, drought resistance is unstable, and yield is caused to reduce and causes shadow to leaf photosynthesis
It rings, and drought resistance agent is mostly that a variety of chemical compositions compound, complicated component, easily causes the residual of growth hormone, environmental safety
It is not high.
Therefore, it is high urgently to find a kind of stability, is not easy to influence the photosynthesis of crop, does not interfere with crop yield more
Drought resistance agent.
The content of the invention
The technical problems to be solved by the invention：It is combined for current using plant growth regulator such as gibberellin etc.
Drought resistance agent, but the residual of growth hormone causes the not high problem of environmental safety, provides a kind of preparation method of drought resistance agent.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method of drought resistance agent, the preparation method include the following steps：
（1）Food and drink waste water is placed in fermentation tank, adjusts pH to 5 ~ 5.5, the high-temperature sterilization at 100 ~ 120 DEG C of temperature is to be cooled
To 30 ~ 32 DEG C, coolant, in mass ratio 1 are obtained：5 ~ 6, bacillus laterosporus seed liquor is added in into coolant, ferment 1 ~ 2d, receives
Collect zymotic fluid, food and drink waste water is placed in fermentation tank, adjust pH to 6 ~ 7, the high-temperature sterilization at 100 ~ 120 DEG C of temperature is to be cooled
To 30 ~ 37 DEG C, coolant a, in mass ratio 1 are obtained：5 ~ 6, bacillus licheniformis seed liquor is added in into coolant a, adjusts pH to 7
~ 8, it is warming up to 40 ~ 45 DEG C, ferment 2 ~ 3d, collects zymotic fluid a, and in mass ratio 2 ~ 6：1, zymotic fluid, zymotic fluid a are mixed,
Obtain mixed liquor；
（2）In mass ratio 1：7 ~ 8, bentonite, modified solution are mixed, stirred at 25 ~ 27 DEG C of temperature, is stood, and from
The heart obtains sediment, and sediment at 100 ~ 110 DEG C of temperature is dried, grinds, obtains abrasive material, in mass ratio 1：10 ~ 15, it will grind
Mill object, mixed liquor are mixed, and stand 5 ~ 6h, and filtering obtains filter residue, and filter residue is dry at 50 ~ 70 DEG C of temperature, obtain dried object；
（3）Count in parts by weight, take 30 ~ 40 parts of water, 10 ~ 15 parts of dried objects, 7 ~ 8 parts of iron chloride, 6 ~ 7 parts of frerrous chlorides, 6 ~ 7 parts
Chitosan solution, 3 ~ 4 parts of ammonium hydroxide, 1 ~ 2 part of glutaraldehyde, dried object, iron chloride, frerrous chloride, water are mixed, ultrasound point
It dissipates, microwave heating after constant temperature stirring, adds chitosan solution, glutaraldehyde, stir, mistake to ammonium hydroxide after 50 ~ 60 DEG C, is added in
Filter, obtains filter residue, filter residue wash 2 ~ 3 times with absolute ethyl alcohol, deionized water, is dried in vacuo at 50 ~ 60 DEG C of temperature, grinding, mistake
Sieve, obtains sieving；
（4）In mass ratio 10 ~ 15：20~25：5~6：1 ~ 2, acrylic acid, sodium hydroxide solution are mixed, after stirring evenly,
Add in acrylamide, humic acid, dissolving be mixed, obtain mixed liquor, count in parts by weight, take 30 ~ 40 parts of deionized waters,
The sweet glycan of 10 ~ 15 portions of konjakus, 7 ~ 8 portions of mixed liquors, 1 ~ 2 part of thiocarbamide, 0.8 ~ 1 part of N, N- methylene-bisacrylamides are sweet by konjaku
Glycan, deionized water mixing under nitrogen protection, in 38 ~ 40 DEG C of stirrings of temperature, add in thiocarbamide, mixed liquor, N, N- di-2-ethylhexylphosphine oxides
Acrylamide is warming up to 60 ~ 65 DEG C, and is stirred to react 3 ~ 4h, obtains reactant, and reactant ethanol solution, water washing must wash
Wash object, in mass ratio 1：3 ~ 4, washings, water are mixed, stirred evenly, stirring mixture is obtained, sieving is preheated
Processing, temperature are 35 ~ 40 DEG C, then are wrapped up the surface for stirring mixture spraying sieving with high-pressure spray-painted rifle, straying quatity
10~20mL/cm3, dried at 60 ~ 70 DEG C to get drought resistance agent.
The step（1）Middle bacillus laterosporus seed liquor is to count in parts by weight, takes 30 ~ 40 parts of water, 10 ~ 15 parts of albumen
Peptone, 10 ~ 15 parts of glucose, 5 ~ 8 parts of yeast extracts are mixed, and high-temperature sterilization obtains culture solution, in mass ratio 1：10 ~ 15, by side spore
Bacillus is seeded in culture solution, and 20 ~ 24 h are cultivated to get side spore gemma bar in 28 ~ 30 DEG C of temperature, 120 ~ 180 r/min
Bacterium seed liquor.
The step（1）Middle bacillus licheniformis seed liquor is to count in parts by weight, takes 30 ~ 40 parts of water, 10 ~ 15 portions of pancreas eggs
White peptone, 10 ~ 15 parts of sodium chloride, 5 ~ 8 parts of yeast extracts are mixed, and high-temperature sterilization obtains culture solution, in mass ratio 1：10 ~ 15,
Bacillus licheniformis is seeded to culture solution, 20 ~ 24 h are cultivated to get lichens bud in 32 ~ 37 DEG C of temperature, 120 ~ 180 r/min
Spore bacillus seed liquor.
The step（2）Middle modified solution is in mass ratio 1：0.8：5 ~ 6, by cetyl trimethylammonium bromide, 12
Sodium alkyl benzene sulfonate, water are mixed, and are stirred evenly to get modified solution.
The step（3）Middle chitosan solution is in mass ratio 1：5 ~ 6, chitosan, acetic acid solution are mixed, gone forward side by side
Row stirring is to get chitosan solution.
Compared with other methods, advantageous effects are the present invention：
（1）On the one hand the present invention realizes the utilization of resources of waste, on the other hand utilizes side spore gemma bar using food and drink waste water as raw material
Bacterium, bacillus licheniformis by fermentation and are mixed with microbial-bacterial fertilizer, and bacillus laterosporus has promoting root growth, sterilization and degradation
The effect of heavy metal, bacillus licheniformis plays an important role of disease-resistant, killing harmful bacteria, so as to which plant is given to provide nutrients
Matter improves the physicochemical property of soil, inhibits the breeding of harmful bacteria and effectively improves crop yield and quality, while and swelling
Soil mixing since bentonite has very big pore volume, specific surface area, so as to possess absorption property, recycles cetyl trimethyl
Ammonium bromide, neopelex are modified bentonite, increase its surface area, improve its absorption property, one side energy
Microbial-bacterial fertilizer is more adsorbed in vivo, on the other hand-the COOH and-OH on its surface contribute to itself and iron ion particle
With reference to,
And iron is the important composition substance of the enzyme of plant aerobic respiration, participates in respiration, is the important object of plant energy metabolism
Matter so that drought resistance agent further promotes the growth of plant, is additionally added chitosan, and chitosan is used as a kind of natural polymer
Its function of being aoxidized is prevented in modification iron ion particle, while chitosan also has anti-microbial property, is avoided that plant is had
The infringement of evil bacterium, therefore the present invention will not cause environmentally friendly sex chromosome mosaicism without any obnoxious growth hormone.
（2）The present invention prepares super strength water absorbent based on the sweet glycan of konjaku, and will be connect using acrylic acid, acrylamide monomer
Branch is on the main chain of the sweet glycan of konjaku, and containing numerous polar groups on strand, hydrophily is very strong, in the environment of active water
In, ionization will occur, generate substantial amounts of charged ion, mutually exclusive due to similar charge, molecular skeleton just expands
It comes, hydrone is just held by storage in the gap of these expansions, so that Plant Drought Resistance improves, due to super strength water absorbent meeting
Hydrogel is formed, so as to wrap up bentonite, after the nutritional ingredient in bentonite first penetrates bentonite, in hydrogel
Free water make nutritional ingredient start dissolving be discharged into again through gel layer in soil, promote crop root part grow.
Specific embodiment
Bacillus laterosporus seed liquor is to count in parts by weight, takes 30 ~ 40 parts of water, 10 ~ 15 parts of peptones, 10 ~ 15 parts of Portugals
Grape sugar, 5 ~ 8 parts of yeast extracts are mixed, and high-temperature sterilization obtains culture solution, in mass ratio 1：10 ~ 15, bacillus laterosporus is inoculated with
Into culture solution, 20 ~ 24 h are cultivated to get bacillus laterosporus seed liquor in 28 ~ 30 DEG C of temperature, 120 ~ 180 r/min.
Bacillus licheniformis seed liquor is to count in parts by weight, take 30 ~ 40 parts of water, 10 ~ 15 parts of tryptones, 10 ~ 15 parts
Sodium chloride, 5 ~ 8 parts of yeast extracts are mixed, and high-temperature sterilization obtains culture solution, in mass ratio 1：10 ~ 15, by lichens gemma bar
Bacterium is seeded to culture solution, and 20 ~ 24 h are cultivated to get bacillus licheniformis seed in 32 ~ 37 DEG C of temperature, 120 ~ 180 r/min
Liquid.
Modified solution is in mass ratio 1：0.8：5 ~ 6, by cetyl trimethylammonium bromide, neopelex,
Water is mixed, and is stirred evenly to get modified solution.
Chitosan solution is in mass ratio 1：5 ~ 6, chitosan, 0.8mol/L acetic acid solutions are mixed, and with 300 ~
400r/min is stirred to get chitosan solution.
A kind of preparation method of drought resistance agent, the preparation method include the following steps：
（1）Food and drink waste water is placed in fermentation tank, adjusts pH to 5 ~ 5.5, at 100 ~ 120 DEG C of temperature high-temperature sterilization 15 ~
20min is cooled to 30 ~ 32 DEG C, obtains coolant, in mass ratio 1：5 ~ 6, bacillus laterosporus seed is added in into coolant
Liquid, ferment 1 ~ 2d, collects zymotic fluid, food and drink waste water is placed in fermentation tank, adjusts pH to 6 ~ 7, high at 100 ~ 120 DEG C of temperature
Temperature 15 ~ 20min of sterilizing, is cooled to 30 ~ 37 DEG C, obtains coolant a, in mass ratio 1：5 ~ 6, lichens bud is added in into coolant a
Spore bacillus seed liquor adjusts pH to 7 ~ 8, is warming up to 40 ~ 45 DEG C, and ferment 2 ~ 3d, collects zymotic fluid a, and in mass ratio 2 ~ 6：1, it will
Zymotic fluid, zymotic fluid a are mixed, and obtain mixed liquor；
（2）In mass ratio 1：7 ~ 8, bentonite, modified solution are mixed, in 25 ~ 27 DEG C of temperature, 200 ~ 300r/min conditions
1 ~ 2h of lower stirring, stand 1 ~ 2h, and with 3000 ~ 4000r/min centrifuge 8 ~ 10min, obtain sediment, by sediment temperature 100 ~
It is dried at 110 DEG C, is ground to 80 ~ 100 mesh, obtain abrasive material, in mass ratio 1：10 ~ 15, abrasive material, mixed liquor are mixed,
5 ~ 6h is stood, filtering obtains filter residue, and filter residue is dry at 50 ~ 70 DEG C of temperature, obtains dried object；
（3）Count in parts by weight, take 30 ~ 40 parts of water, 10 ~ 15 parts of dried objects, 7 ~ 8 parts of iron chloride, 6 ~ 7 parts of frerrous chlorides, 6 ~ 7 parts
Chitosan solution, 3 ~ 4 parts of 0.8mol/L ammonium hydroxide, 1 ~ 2 part of glutaraldehyde, dried object, iron chloride, frerrous chloride, water are mixed,
8 ~ 10min of ultrasonic disperse, microwave heating after constant temperature stirs 25 ~ 30 min, add shell and gather to ammonium hydroxide after 50 ~ 60 DEG C, is added in
Sugar juice, glutaraldehyde stir 25 ~ 30 min with 200 ~ 300r/min, and filtering obtains filter residue, by filter residue absolute ethyl alcohol, deionization
Water washing 2 ~ 3 times, is dried in vacuo 10 ~ 12h at 50 ~ 60 DEG C of temperature, and grinding crosses 150 ~ 200 mesh sieves, obtains sieving；
（4）In mass ratio 10 ~ 15：20~25：5~6：1 ~ 2, acrylic acid, sodium hydroxide solution are mixed, after stirring evenly,
Add in acrylamide, humic acid, dissolving be mixed, obtain mixed liquor, count in parts by weight, take 30 ~ 40 parts of deionized waters,
The sweet glycan of 10 ~ 15 portions of konjakus, 7 ~ 8 portions of mixed liquors, 1 ~ 2 part of thiocarbamide, 0.8 ~ 1 part of N, N- methylene-bisacrylamides are sweet by konjaku
Glycan, deionized water mixing under nitrogen protection, in 38 ~ 40 DEG C of 0.8 ~ 1 h of stirring of temperature, add in thiocarbamide, mixed liquor, N, N-
Methylene-bisacrylamide is warming up to 60 ~ 65 DEG C, and is stirred to react 3 ~ 4h with 300 ~ 400r/min, obtains reactant, by reactant
With 0.8mol/L ethanol solutions, water washing, washings, in mass ratio 1 are obtained：3 ~ 4, washings, water are mixed, stirring is equal
It is even, stirring mixture is obtained, sieving is subjected to the pre-heat treatment, temperature is 35 ~ 40 DEG C, then will stir mixture with high-pressure spray-painted rifle
The surface of spraying sieving is wrapped up, 10 ~ 20mL/cm of straying quatity3, dry 2 ~ 3h is to get drought resistance agent at 60 ~ 70 DEG C.
Embodiment 1
Bacillus laterosporus seed liquor is to count in parts by weight, takes 30 parts of water, 10 parts of peptones, 10 parts of glucose, 5 parts of yeast extracts
It is mixed, high-temperature sterilization, obtains culture solution, in mass ratio 1：10, bacillus laterosporus is seeded in culture solution, in temperature 28
DEG C, 120 r/min cultivate 20 h to get bacillus laterosporus seed liquor.
Bacillus licheniformis seed liquor is to count in parts by weight, take 30 parts of water, 10 parts of tryptones, 10 parts of sodium chloride, 5 parts
Yeast extract is mixed, high-temperature sterilization, obtains culture solution, in mass ratio 1：10, bacillus licheniformis is seeded to culture solution,
20 h are cultivated to get bacillus licheniformis seed liquor in 32 DEG C of temperature, 120 r/min.
Modified solution is in mass ratio 1：0.8：5, by cetyl trimethylammonium bromide, neopelex, water
It is mixed, is stirred evenly to get modified solution.
Chitosan solution is in mass ratio 1：5, chitosan, 0.8mol/L acetic acid solutions are mixed, and with 300r/
Min is stirred to get chitosan solution.
A kind of preparation method of drought resistance agent, the preparation method include the following steps：
（2）Food and drink waste water is placed in fermentation tank, adjusts pH to 5, the high-temperature sterilization 15min at 100 DEG C of temperature is cooled to 30
DEG C, obtain coolant, in mass ratio 1：5, bacillus laterosporus seed liquor is added in into coolant, ferment 1d, collects zymotic fluid, will
Food and drink waste water is placed in fermentation tank, adjusts pH to 6, and the high-temperature sterilization 15min at 100 DEG C of temperature is cooled to 30 DEG C, must cool down
Liquid a, in mass ratio 1：5, bacillus licheniformis seed liquor is added in into coolant a, adjusts pH to 7, is warming up to 40 DEG C, fermentation
2d, collect zymotic fluid a, in mass ratio 2：1, zymotic fluid, zymotic fluid a are mixed, obtain mixed liquor；
（2）In mass ratio 1：7, bentonite, modified solution are mixed, in 25 DEG C of temperature, 1h is stirred under the conditions of 200r/min,
1h is stood, and 8min is centrifuged with 3000r/min, obtains sediment, sediment at 100 DEG C of temperature is dried, 80 mesh is ground to, obtains
Abrasive material, in mass ratio 1：10, abrasive material, mixed liquor are mixed, stand 5h, filtering obtains filter residue, by filter residue in temperature 50
It is dry at DEG C, obtain dried object；
（3）It counts in parts by weight, takes 30 parts of water, 10 parts of dried objects, 7 parts of iron chloride, 6 parts of frerrous chlorides, 6 parts of chitosan solutions, 3
Part 0.8mol/L ammonium hydroxide, 1 part of glutaraldehyde, dried object, iron chloride, frerrous chloride, water are mixed, ultrasonic disperse 8min, micro-
Wave heating after constant temperature stirring 25min, is added chitosan solution, glutaraldehyde, is stirred with 200r/min to ammonium hydroxide after 50 DEG C, is added in
25min is mixed, is filtered, is obtained filter residue, filter residue with absolute ethyl alcohol, deionized water is washed 2 times, is dried in vacuo under temperature 50 C
10h, grinding cross 150 mesh sieves, obtain sieving；
（4）In mass ratio 10：20：5：1, acrylic acid, sodium hydroxide solution are mixed, after stirring evenly, add in acryloyl
Amine, humic acid, dissolving are mixed, obtain mixed liquor, count in parts by weight, take 30 parts of deionized waters, 10 portions of konjakus sweet poly-
Sugar, 7 portions of mixed liquors, 1 part of thiocarbamide, 0.8 part of N, N- methylene-bisacrylamides mix the sweet glycan of konjaku, deionized water, in nitrogen
Under gas shielded, in 38 DEG C of stirring 0.8h of temperature, thiocarbamide, mixed liquor, N are added in, N- methylene-bisacrylamides are warming up to 60 DEG C,
And 3h is stirred to react with 300r/min, reactant is obtained, by reactant 0.8mol/L ethanol solutions, water washing, washings is obtained, presses
Mass ratio 1：3, washings, water are mixed, stirred evenly, obtains stirring mixture, sieving is subjected to the pre-heat treatment, temperature
For 35 DEG C, then with high-pressure spray-painted rifle by stir mixture spraying sieving surface wrap up, straying quatity 10mL/cm3, 60
Dry 2h is to get drought resistance agent at DEG C.
Embodiment 2
Bacillus laterosporus seed liquor is to count in parts by weight, takes 35 parts of water, 13 parts of peptones, 13 parts of glucose, 6 parts of yeast extracts
It is mixed, high-temperature sterilization, obtains culture solution, in mass ratio 1：13, bacillus laterosporus is seeded in culture solution, in temperature 29
DEG C, 160 r/min cultivate 22 h to get bacillus laterosporus seed liquor.
Bacillus licheniformis seed liquor is to count in parts by weight, take 35 parts of water, 13 parts of tryptones, 13 parts of sodium chloride, 6 parts
Yeast extract is mixed, high-temperature sterilization, obtains culture solution, in mass ratio 1：13, bacillus licheniformis is seeded to culture solution,
22 h are cultivated to get bacillus licheniformis seed liquor in 35 DEG C of temperature, 160 r/min.
Modified solution is in mass ratio 1：0.8：5, by cetyl trimethylammonium bromide, neopelex, water
It is mixed, is stirred evenly to get modified solution.
Chitosan solution is in mass ratio 1：5, chitosan, 0.8mol/L acetic acid solutions are mixed, and with 350r/
Min is stirred to get chitosan solution.
A kind of preparation method of drought resistance agent, the preparation method include the following steps：
（3）Food and drink waste water is placed in fermentation tank, adjusts pH to 5.2, the high-temperature sterilization 17min at 110 DEG C of temperature is cooled to
31 DEG C, obtain coolant, in mass ratio 1：5, bacillus laterosporus seed liquor is added in into coolant, ferment 1d, collects zymotic fluid,
Food and drink waste water is placed in fermentation tank, adjusts pH to 6, the high-temperature sterilization 17min at 110 DEG C of temperature is cooled to 35 DEG C, obtains cold
But liquid a, in mass ratio 1：5, bacillus licheniformis seed liquor is added in into coolant a, adjusts pH to 7, is warming up to 43 DEG C, fermentation
2d, collect zymotic fluid a, in mass ratio 4：1, zymotic fluid, zymotic fluid a are mixed, obtain mixed liquor；
（2）In mass ratio 1：7, bentonite, modified solution are mixed, in 26 DEG C of temperature, 1h is stirred under the conditions of 250r/min,
1h is stood, and 9min is centrifuged with 3500r/min, obtains sediment, sediment at 105 DEG C of temperature is dried, 90 mesh is ground to, obtains
Abrasive material, in mass ratio 1：13, abrasive material, mixed liquor are mixed, stand 5h, filtering obtains filter residue, by filter residue in temperature 55
It is dry at DEG C, obtain dried object；
（3）It counts in parts by weight, takes 35 parts of water, 13 parts of dried objects, 7 parts of iron chloride, 6 parts of frerrous chlorides, 6 parts of chitosan solutions, 3
Part 0.8mol/L ammonium hydroxide, 1 part of glutaraldehyde, dried object, iron chloride, frerrous chloride, water are mixed, ultrasonic disperse 9min, micro-
Wave heating after constant temperature stirring 27min, is added chitosan solution, glutaraldehyde, is stirred with 250r/min to ammonium hydroxide after 55 DEG C, is added in
27 min are mixed, is filtered, is obtained filter residue, filter residue is washed 2 times with absolute ethyl alcohol, deionized water, are dried in vacuo at 55 DEG C of temperature
11h, grinding cross 170 mesh sieves, obtain sieving；
（4）In mass ratio 12：22：5：1, acrylic acid, sodium hydroxide solution are mixed, after stirring evenly, add in acryloyl
Amine, humic acid, dissolving are mixed, obtain mixed liquor, count in parts by weight, take 35 parts of deionized waters, 12 portions of konjakus sweet poly-
Sugar, 7 portions of mixed liquors, 1 part of thiocarbamide, 0.9 part of N, N- methylene-bisacrylamides mix the sweet glycan of konjaku, deionized water, in nitrogen
Under gas shielded, in 39 DEG C of stirring 0.9h of temperature, thiocarbamide, mixed liquor, N are added in, N- methylene-bisacrylamides are warming up to 63 DEG C,
And 3h is stirred to react with 350r/min, reactant is obtained, by reactant 0.8mol/L ethanol solutions, water washing, washings is obtained, presses
Mass ratio 1：3, washings, water are mixed, stirred evenly, obtains stirring mixture, sieving is subjected to the pre-heat treatment, temperature
For 37 DEG C, then with high-pressure spray-painted rifle by stir mixture spraying sieving surface wrap up, straying quatity 15mL/cm3, 65
Dry 2h is to get drought resistance agent at DEG C.
Embodiment 3
Bacillus laterosporus seed liquor is to count in parts by weight, takes 40 parts of water, 15 parts of peptones, 15 parts of glucose, 8 parts of yeast extracts
It is mixed, high-temperature sterilization, obtains culture solution, in mass ratio 1：15, bacillus laterosporus is seeded in culture solution, in temperature 30
DEG C, 180 r/min cultivate 24 h to get bacillus laterosporus seed liquor.
Bacillus licheniformis seed liquor is to count in parts by weight, take 40 parts of water, 15 parts of tryptones, 15 parts of sodium chloride, 8 parts
Yeast extract is mixed, high-temperature sterilization, obtains culture solution, in mass ratio 1：15, bacillus licheniformis is seeded to culture solution,
24 h are cultivated to get bacillus licheniformis seed liquor in 37 DEG C of temperature, 180 r/min.
Modified solution is in mass ratio 1：0.8：6, by cetyl trimethylammonium bromide, neopelex, water
It is mixed, is stirred evenly to get modified solution.
Chitosan solution is in mass ratio 1：6, chitosan, 0.8mol/L acetic acid solutions are mixed, and with 400r/
Min is stirred to get chitosan solution.
A kind of preparation method of drought resistance agent, the preparation method include the following steps：
（4）Food and drink waste water is placed in fermentation tank, adjusts pH to 5.5, the high-temperature sterilization 20min at 120 DEG C of temperature is cooled to
32 DEG C, obtain coolant, in mass ratio 1：6, bacillus laterosporus seed liquor is added in into coolant, ferment 2d, collects zymotic fluid,
Food and drink waste water is placed in fermentation tank, adjusts pH to 7, the high-temperature sterilization 20min at 120 DEG C of temperature is cooled to 37 DEG C, obtains cold
But liquid a, in mass ratio 1：6, bacillus licheniformis seed liquor is added in into coolant a, adjusts pH to 8, is warming up to 45 DEG C, fermentation
3d, collect zymotic fluid a, in mass ratio 6：1, zymotic fluid, zymotic fluid a are mixed, obtain mixed liquor；
（2）In mass ratio 1：8, bentonite, modified solution are mixed, in 27 DEG C of temperature, 2h is stirred under the conditions of 300r/min,
2h is stood, and 10min is centrifuged with 4000r/min, obtains sediment, sediment at 110 DEG C of temperature is dried, is ground to 100 mesh,
Abrasive material, in mass ratio 1：15, abrasive material, mixed liquor are mixed, stand 6h, filtering obtains filter residue, by filter residue in temperature
It is dry at 70 DEG C, obtain dried object；
（3）It counts in parts by weight, takes 40 parts of water, 15 parts of dried objects, 8 parts of iron chloride, 7 parts of frerrous chlorides, 7 parts of chitosan solutions, 4
Part 0.8mol/L ammonium hydroxide, 2 parts of glutaraldehydes, dried object, iron chloride, frerrous chloride, water are mixed, ultrasonic disperse 10min, micro-
Wave heating after constant temperature stirs 30 min, is added chitosan solution, glutaraldehyde, is stirred with 300r/min to ammonium hydroxide after 60 DEG C, is added in
30 min are mixed, is filtered, is obtained filter residue, filter residue with absolute ethyl alcohol, deionized water is washed 3 times, is dried in vacuo under temperature 60 C
12h, grinding cross 200 mesh sieves, obtain sieving；
（4）In mass ratio 15：25：6：2, acrylic acid, sodium hydroxide solution are mixed, after stirring evenly, add in acryloyl
Amine, humic acid, dissolving are mixed, obtain mixed liquor, count in parts by weight, take 40 parts of deionized waters, 15 portions of konjakus sweet poly-
Sugar, 8 portions of mixed liquors, 2 parts of thiocarbamides, 1 part of N, N- methylene-bisacrylamides mix the sweet glycan of konjaku, deionized water, in nitrogen
Under protection, in 40 DEG C of temperature, 1 h of stirring, thiocarbamide, mixed liquor, N are added in, N- methylene-bisacrylamides are warming up to 65 DEG C, and with
400r/min is stirred to react 4h, obtains reactant, by reactant 0.8mol/L ethanol solutions, water washing, washings is obtained, by quality
Than 1：4, washings, water are mixed, stirred evenly, obtains stirring mixture, sieving is subjected to the pre-heat treatment, temperature 40
DEG C, then wrapped up the surface for stirring mixture spraying sieving with high-pressure spray-painted rifle, straying quatity 20mL/cm3, at 70 DEG C
Dry 3h is to get drought resistance agent.
Comparative example：The drought resistance agent of Liuzhou bio tech ltd production
Method：The watermelon farmland of one piece of 4 mu of size is chosen, its decile is divided into 4 groups, per acre as laboratory sample
There are 100 plants of watermelons.
In mass ratio 1:100, the drought resistance agent prepared by embodiment and comparative example with water is stirred after mixing, is obtained
To dilution drought resistance agent, dilution drought resistance agent is uniformly sprayed on soil surface, the amount for controlling sprinkling is 100kg/ mus；Every 10 days
It sprays once, amounts to sprinkling twice.And embodiment and the drought resisting prepared by comparative example are detected according to standard GB 20693-2006
Agent characterizes the height of its performance with this to the growth regulating effect of watermelon.
After the test, the embodiment group of equivalent and the soil of comparative example group are taken, is detected using gas chromatography in soil
Growth hormone residual quantity.
The specific detection case of drought resistance agent such as table 1
Table 1
Note：Before experiment, the average length of farmland watermelon main root is 10cm, and the climing average length of melon is 25cm.
From the foregoing, it will be observed that the drought resistance agent prepared by the present invention can effectively solve the growth that present drought resistance agent has
The defects of hormone residues amount is excessive, and can preferably promote the production of plant, in addition, the drought resistance agent prepared by the present invention is adopted
Raw material is the substance of safety non-pollution, is a kind of safety and the preparation method of efficient drought resistance agent, is worthy to be popularized and makes
With.
Claims (5)
1. a kind of preparation method of drought resistance agent, which is characterized in that the preparation method includes the following steps：
（1）Food and drink waste water is placed in fermentation tank, adjusts pH to 5 ~ 5.5, the high-temperature sterilization at 100 ~ 120 DEG C of temperature is to be cooled
To 30 ~ 32 DEG C, coolant, in mass ratio 1 are obtained：5 ~ 6, bacillus laterosporus seed liquor is added in into coolant, ferment 1 ~ 2d, receives
Collect zymotic fluid, food and drink waste water is placed in fermentation tank, adjust pH to 6 ~ 7, the high-temperature sterilization at 100 ~ 120 DEG C of temperature is to be cooled
To 30 ~ 37 DEG C, coolant a, in mass ratio 1 are obtained：5 ~ 6, bacillus licheniformis seed liquor is added in into coolant a, adjusts pH to 7
~ 8, it is warming up to 40 ~ 45 DEG C, ferment 2 ~ 3d, collects zymotic fluid a, and in mass ratio 2 ~ 6：1, zymotic fluid, zymotic fluid a are mixed,
Obtain mixed liquor；
（2）In mass ratio 1：7 ~ 8, bentonite, modified solution are mixed, stirred at 25 ~ 27 DEG C of temperature, is stood, and from
The heart obtains sediment, and sediment at 100 ~ 110 DEG C of temperature is dried, grinds, obtains abrasive material, in mass ratio 1：10 ~ 15, it will grind
Mill object, mixed liquor are mixed, and stand 5 ~ 6h, and filtering obtains filter residue, and filter residue is dry at 50 ~ 70 DEG C of temperature, obtain dried object；
（3）Count in parts by weight, take 30 ~ 40 parts of water, 10 ~ 15 parts of dried objects, 7 ~ 8 parts of iron chloride, 6 ~ 7 parts of frerrous chlorides, 6 ~ 7 parts
Chitosan solution, 3 ~ 4 parts of ammonium hydroxide, 1 ~ 2 part of glutaraldehyde, dried object, iron chloride, frerrous chloride, water are mixed, ultrasound point
It dissipates, microwave heating after constant temperature stirring, adds chitosan solution, glutaraldehyde, stir, mistake to ammonium hydroxide after 50 ~ 60 DEG C, is added in
Filter, obtains filter residue, filter residue wash 2 ~ 3 times with absolute ethyl alcohol, deionized water, is dried in vacuo at 50 ~ 60 DEG C of temperature, grinding, mistake
Sieve, obtains sieving；
（4）In mass ratio 10 ~ 15：20~25：5~6：1 ~ 2, acrylic acid, sodium hydroxide solution are mixed, after stirring evenly,
Add in acrylamide, humic acid, dissolving be mixed, obtain mixed liquor, count in parts by weight, take 30 ~ 40 parts of deionized waters,
The sweet glycan of 10 ~ 15 portions of konjakus, 7 ~ 8 portions of mixed liquors, 1 ~ 2 part of thiocarbamide, 0.8 ~ 1 part of N, N- methylene-bisacrylamides are sweet by konjaku
Glycan, deionized water mixing under nitrogen protection, in 38 ~ 40 DEG C of stirrings of temperature, add in thiocarbamide, mixed liquor, N, N- di-2-ethylhexylphosphine oxides
Acrylamide is warming up to 60 ~ 65 DEG C, and is stirred to react 3 ~ 4h, obtains reactant, and reactant ethanol solution, water washing must wash
Wash object, in mass ratio 1：3 ~ 4, washings, water are mixed, stirred evenly, stirring mixture is obtained, sieving is preheated
Processing, temperature are 35 ~ 40 DEG C, then are wrapped up the surface for stirring mixture spraying sieving with high-pressure spray-painted rifle, straying quatity
10~20mL/cm3, dried at 60 ~ 70 DEG C to get drought resistance agent.
2. the preparation method of drought resistance agent according to claim 1, which is characterized in that the step（1）Middle bacillus laterosporus
Seed liquor is to count in parts by weight, and 30 ~ 40 parts of water, 10 ~ 15 parts of peptones, 10 ~ 15 parts of glucose, 5 ~ 8 parts of yeast extracts is taken to carry out
Mixing, high-temperature sterilization obtain culture solution, in mass ratio 1：10 ~ 15, bacillus laterosporus is seeded in culture solution, temperature 28 ~
30 DEG C, 120 ~ 180 r/min cultivate 20 ~ 24 h to get bacillus laterosporus seed liquor.
3. the preparation method of drought resistance agent according to claim 1, which is characterized in that the step（1）Middle bacillus licheniformis
Seed liquor is to count in parts by weight, takes 30 ~ 40 parts of water, 10 ~ 15 parts of tryptones, 10 ~ 15 parts of sodium chloride, 5 ~ 8 parts of yeast extractions
Object is mixed, high-temperature sterilization, obtains culture solution, in mass ratio 1：10 ~ 15, bacillus licheniformis is seeded to culture solution, in temperature
32 ~ 37 DEG C of degree, 120 ~ 180 r/min cultivate 20 ~ 24 h to get bacillus licheniformis seed liquor.
4. the preparation method of drought resistance agent according to claim 1, which is characterized in that the step（2）Middle modified solution be by
Mass ratio 1：0.8：5 ~ 6, cetyl trimethylammonium bromide, neopelex, water are mixed, stirred evenly,
Up to modified solution.
5. the preparation method of drought resistance agent according to claim 1, which is characterized in that the step（3）Middle chitosan solution is
In mass ratio 1：5 ~ 6, chitosan, acetic acid solution are mixed, and are stirred to get chitosan solution.
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Application publication date: 20180522 |