CN108013037B - A method of increasing female flower of cucumber rate using epiphysin - Google Patents
A method of increasing female flower of cucumber rate using epiphysin Download PDFInfo
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- CN108013037B CN108013037B CN201711349520.3A CN201711349520A CN108013037B CN 108013037 B CN108013037 B CN 108013037B CN 201711349520 A CN201711349520 A CN 201711349520A CN 108013037 B CN108013037 B CN 108013037B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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Abstract
The invention discloses a kind of methods for increasing female flower of cucumber rate using epiphysin, it is that the epiphysin solution of 10~50 μm of ol/L sprays application to the blade of cucumber seedling, stem apex and perhaps on complete stool or liquid-transfering gun used to be injected on the stem apex of cucumber seedling and blade also in growth the method includes using concentration.Cucumber the first female flower section can be effectively reduced by being handled using epiphysin cucumber seedling using the solution of the present invention, promote cucumber precocious；Increase the female-flower rate of cucumber, the female flower ratio up-regulation 10~20% of preceding 20 section peomotes cucumber increment, it is contemplated that amount of increase in production can reach 20% or so.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to a method of increase female flower of cucumber rate using epiphysin.
Background technique
Gourd vegetable crop is female, the male different flower of homophyletic, and melon spending can be divided into female flower, male flower and hermaphrodite flower, three kinds of flowers
Bud is all developed in both sexes flower primordium.When environmental condition, which is suitable for gynoecium former base, develops, stamen retrogressive is degenerated, the development of gynoecium former base,
Form female flower；When environmental condition is suitable for stamen retrogressive development, gynoecium former base is degenerated, and stamen retrogressive development just forms male flower.Due to
Sex Differentiation directly affects melon yield and quality, and the increase of especially female flower ratio is the important channel for promoting volume increase.
Inherent cause is to influence the principal element of role in sex expression of cucumber.The Sex Determination of cucumber is by 3 major gene resistances A, F, M
It is codetermined with 4 minor genes In-F, m-2, Tr, gy.External environment and hormone are also influence melon Sex Differentiation important
Factor.Section reduction that the short-day of usual low temperature especially low tone night mild 8h or so is conducive to Female flower differentiation and female flower is blossomed.
Plant hormone is the important regulating and controlling factor of Plant Sex Differentiation.Ethylene increases female flower more, and gibberellin increases male flower more.In cucumber 2
~5 leaf phases sprayed 1~3 time using 150ppm ethephon (CEPHA),2-(chloroethyl) phosphonic acid on blade can promote female flower ratio to rise, but ethephon (CEPHA),2-(chloroethyl) phosphonic acid has inhibition to give birth to
The defect of long effect.And gibberellin processing cucumber seedling promotes male flower to be formed, and have inhibiting effect to the formation of female flower.To Huang
Melon processing brassinosteroid (BR) can be such that First female flower reduces, and increase the Female Flower Number on stem, mainly BR regulation
Melon Sex Differentiation is to pass through ethylene signaling pathway.In addition auxin (IAA) and abscisic acid (ABA) can promote to a certain extent
Break up into cucumber female.But whether epiphysin participates in melon Sex Differentiation and it is not immediately clear.
Summary of the invention
The purpose of the present invention is to provide a kind of method for increasing female flower of cucumber rate using epiphysin, the method handles energy
Cucumber the first female flower section can enough be effectively reduced, increase the female-flower rate of cucumber, promote cucumber increment.
The present invention provides a kind of method for increasing female flower of cucumber rate using epiphysin, is 10~50 μm of ol/L to use concentration
Epiphysin solution cucumber seedling is handled.
Preferably, the blade of cucumber seedling, stem apex are sprayed application to using epiphysin solution and perhaps on complete stool or uses liquid relief
The stem apex and the blade also in growth that rifle is injected into cucumber seedling.
Preferably, the preparation method of the epiphysin solution are as follows: obtain epiphysin mother liquor with alcohol dissolution epiphysin, then
Epiphysin mother liquor is diluted with water again and obtains epiphysin solution.
Preferably, the cucumber seedling in the cucumber seedling refer to a leaf wholeheartedly period to four leaves wholeheartedly period.
Preferably, the processing time is at dusk or night.Due to the light-exposed easy decomposition of epiphysin, thus selection is being close to
Evening or night are handled.
Preferably, the number of processing is 2~5 times, is handled 1 time every l~3 day.
Preferably, in one leaf of cucumber seedling, wholeheartedly period, wholeheartedly period used taking off for 10~50 μm of ol/l concentration black to four leaves
Plain solution sprays on the blade, stem apex or complete stool of cucumber seedling, until flowing water, spraying time are at dusk that spraying number is 2
It~3 times, is sprayed once every l~3 day.
Processing energy is carried out to cucumber seedling using epiphysin compared with prior art, the present invention having the beneficial effect that
Cucumber the first female flower section can enough be effectively reduced, improve the prematureness of cucumber, cucumber is promoted to list ahead of time；Increase the female flower of cucumber
Rate, the female flower ratio of preceding 20 section raise 10-20%, may advantageously facilitate cucumber increment, it is contemplated that amount of increase in production can reach 20% with
On.
Specific embodiment
The invention discloses a kind of method for increasing female flower of cucumber rate using epiphysin, those skilled in the art can be used for reference
Present disclosure is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to this field skill
It is it will be apparent that they are considered as being included in the present invention for art personnel.Method of the invention and application by compared with
Good embodiment is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to as described herein
Methods and applications are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
Experimental method in following embodiments is unless otherwise instructed conventional method.The reagent that following embodiments are related to
Or raw material is unless otherwise noted, is common commercially available product, can all be bought and be obtained by market.
Epiphysin (Melatonin, MT) is a kind of hormone, is to find for the first time in the pineal body of animal, is prevalent in
In animal and plant and microorganism.Epiphysin participates in the regulation photoperiod；Its function similar to auxin, can promote isolated cells expand and
The regeneration of lateral root and adventitious root；Free radical can also be removed；As antioxidant, it can effectively inhibit arid, UV-B, heavy metal-polluted
Injury of the adverse environmental factors such as dye, suppression harmful bacteria, high temperature and low temperature to plant cell.
Below by embodiment, the present invention is described in detail.
Embodiment 1:
(1) cucumber seedling is cultivated
The cucumber variety for selecting percentage of seedgermination high, removal granule, empty grain, broken kernel and impurity, indoor sunning 2 before soaking seed
It.Then it is sterilized ten minutes using ready 5%NaClO in advance, is then used distilled water repeated flushing 5 times；Or using temperature
Soup seed soaking, soak seed 15min~20min in 50 DEG C~55 DEG C hot water, is continuously agitated, then with 30 DEG C of water seed soaking 6h~8h,
It rubs the impurity gone on the surface of the seed with the hands repeatedly, cleans up vernalization.Keep seed wet after seed soaking, in 25 DEG C~30 DEG C vernalization 20h
Left and right is cleaned 1 time or 2 times, therebetween until sowing when seed 70% shows money or valuables one carries unintentionally.Then seed is sowed in nutritive cube or hole plate seedling growth
In.Slightly below nursery hole or nutritive cube, every cave (alms bowl) sow 1, seed are laid flat when Nutrition Soil or medium in tray (alms bowl), bud point
Downwards, it is equably watered with bottom water after finishing, then covers the Nutrition Soil of 0.5cm~1cm.
The growing environment for guaranteeing cucumber is to control at 20 DEG C~28 DEG C daytime, and night temperature control is at 15 DEG C~18 DEG C；It is especially late
The short-day that upper low temperature is maintained at 13 DEG C~15 DEG C of low night temperature, and keeps 8h or so is more conducive to the opening of female flower.
(2) epiphysin preparation prepares
The epiphysin preparation of 4 concentration gradients (1 μm of ol/L, 10 μm of ol/L, 50 μm of ol/L, 100 μm of ol/L) is set.
Preparation steps: being dissolved with a small amount of alcohol first, and epiphysin concentration required for being then diluted with water to again owns
Operation is carried out in the dark as far as possible, and configured preparation is kept in dark place.
In the present embodiment, the epiphysin can be dissolved as long as preparing the dosage of alcohol in epiphysin mother liquor,
The fewer the dosage of alcohol the better, and those skilled in the art can obtain dissolution according to Calculation of Solubility of the epiphysin in alcohol and take off
The usage amount of alcohol needed for melanocyte.
(3) processing experiment
The epiphysin preparation of 4 concentration gradients of experimental setup (1 μm of ol/L, 10 μm of ol/L, 50 μm of ol/L, 100 μm of ol/L)
As experimental group, and the clear water without epiphysin is set as blank control.
One leaf of cucumber seedling wholeheartedly when epiphysin solution sprayed in the seedling leaves of cucumber, until flowing water, takes off black
Plain solution sprayed once every l days, and spraying time is that at dusk, spraying number is 3 times.Then statistics first time female flower section is with before
20 section female flower ratios.The results are shown in Table 1:
Table 1: influence of the epiphysin various concentration to cucumber first time female flower section and preceding 20 section female flower ratio
As a result illustrate: the Statistical Studies On The Variations of the data application ANOVA of table 1 are analyzed, group difference is significant (P < 0.05), uses
Tukey analysis means carry out variance analysis, with difference of the different alphabet registrations between.What a, b, c were represented in table 1 is different
It whether there is difference between processing.
By experimental group 3 known to table 1 and experimental group 4, difference is maximum compared with blank control group, is characterized in particular in: 1. from first
Secondary female flower section sees decline by a relatively large margin, is conducive to improve the cucumber precocious time；2. preceding 20 section female flower ratio has significantly
Promotion, ascensional range reaches 10-20%, is conducive to the yield for improving cucumber, it is contemplated that can increase cucumber yield 20%.
It is surprisingly found by the inventors that experimental group 2 is compared with blank control group, first time female flower section and preceding 20 section female flower ratio
Example difference compared with blank control group is not obvious enough；Experimental group 5 is compared with blank control group, and first time female flower section has by a small margin
It reduces, but difference is not significant, preceding 20 section female flower ratio is without significant difference.
Embodiment 2
Step (1)~(2) are four leaves wholeheartedly period in step (3) with embodiment 1, statistics first time female flower section and before
20 section female flower ratios, can reach the treatment effect of embodiment 1 as the result is shown.
Embodiment 3
With embodiment 1, the number that sprays in step (3) is 2 times for step (1)~(2), statistics first time female flower section and before
20 section female flower ratios, can reach the treatment effect of embodiment 1 as the result is shown.
Embodiment 4
Step (1)~(2) sprayed an epiphysin solution with embodiment 1 every 3 days in step (3), statistics is female for the first time
Flower section and preceding 20 section female flower ratio, can reach the treatment effect of embodiment 1 as the result is shown.
Embodiment 5
Step (1)~(2) are with embodiment 1, and processing method is the stem that cucumber seedling is injected into using liquid-transfering gun in step (3)
Point and blade also in growth, the injection volume of every cucumber seedling is different with the size of plant, and injection volume is 250 microlitres~
1000 microlitres, profit stem apex and blade are all invaded, first time female flower section and preceding 20 section female flower ratio is counted, as the result is shown can
Enough reach the treatment effect of embodiment 1.
Claims (6)
1. a kind of method for increasing female flower of cucumber rate using epiphysin, which is characterized in that the use of concentration to be 10~50 μm of ol/L
Epiphysin solution cucumber seedling is handled.
2. a kind of method for increasing female flower of cucumber rate using epiphysin according to claim 1, which is characterized in that use
Epiphysin solution sprays application to the blade of cucumber seedling, stem apex and is injected into cucumber seedling perhaps on complete stool or using liquid-transfering gun
Stem apex and the blade also in growth.
3. a kind of method for increasing female flower of cucumber rate using epiphysin according to claim 1, which is characterized in that described to take off
The preparation method of melanocyte solution are as follows: obtain epiphysin mother liquor with alcohol dissolution epiphysin, epiphysin mother liquor is then diluted with water again
Obtain epiphysin solution.
4. a kind of method for increasing female flower of cucumber rate using epiphysin according to claim 1, which is characterized in that the Huang
The cucumber seedling in melon seedling refers to a leaf wholeheartedly period to four leaves wholeheartedly period.
5. a kind of method for increasing female flower of cucumber rate using epiphysin according to claim 1, which is characterized in that the place
The number of reason is 2~5 times, is handled 1 time every l~3 day.
6. a kind of method for increasing female flower of cucumber rate using epiphysin according to claim 1, which is characterized in that in cucumber
Wholeheartedly period, wholeheartedly period used the epiphysin solution of 10~50 μm of ol/l concentration to spray in cucumber seedling to one leaf of seedling to four leaves
Blade, on stem apex or complete stool, until flowing water, spraying time are at dusk that spraying number is 2~3 times, is sprayed every l~3 day
Once.
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| CN108925566A (en) * | 2018-06-20 | 2018-12-04 | 聊城职业技术学院 | Epiphysin is alleviating the application in greenhouse cucumber continuous cropping obstacle |
| CN111670669A (en) * | 2020-07-22 | 2020-09-18 | 西北农林科技大学 | Fertilizing and irrigating method for promoting apple fruit expansion |
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| US5300481A (en) * | 1993-05-05 | 1994-04-05 | The Regents Of The University Of California | Methods for controlling flowering in plants |
| CN101347115A (en) * | 2008-07-03 | 2009-01-21 | 浙江大学 | Formulation for improving quantity production of bitter melon |
| CN104412979B (en) * | 2013-08-19 | 2016-04-13 | 中国科学院遗传与发育生物学研究所 | The novelty teabag of epiphysin |
| CN104823738B (en) * | 2015-04-13 | 2017-06-16 | 浙江大学 | Epiphysin is reducing the application in plant in Cd accumulation |
| CN105104046B (en) * | 2015-09-08 | 2018-03-16 | 海南大学 | A kind of method that epiphysin induction processing improves rice bacterial blight resistance |
| CN105900989B (en) * | 2016-04-18 | 2018-03-16 | 浙江大学 | Application of the epiphysin in improving vegetables thionin and absorbing |
| CN106070306A (en) * | 2016-06-14 | 2016-11-09 | 韦锦鑫 | A kind of method improving autumn cucumber seat melon rate |
| CN106748171A (en) * | 2016-12-27 | 2017-05-31 | 中国热带农业科学院热带作物品种资源研究所 | A kind of balsam pear promotees flower and promotees fruit foliar fertilizer and its preparation method and application |
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