CN107998257B - Traditional Chinese medicine composition for treating turkey histomoniasis and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for treating turkey histomoniasis and preparation method and application thereof Download PDFInfo
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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Abstract
A traditional Chinese medicine composition for treating histomoniasis of turkeys is characterized by comprising the following raw materials in parts by weight: 1-3 parts of gamboge, 5-10 parts of cyrtomium rhizome, 8-12 parts of agrimony bud, 5-10 parts of pepper and 10-15 parts of aloe. The traditional Chinese medicine composition has reasonable compatibility, gamboge and cyrtomium rhizome are used as monarch drugs for clearing heat and removing toxicity and resisting bacteria and killing insects, the gemma agrimoninae and pepper are used as ministerial drugs for expelling insects and killing insects, the aloe, the angelica dahurica, the red paeony root and the subprostrate sophora are used as assistants for clearing liver, cooling blood and relieving swelling and pain, the platycladi seed and the cortex albiziae are used as conductant drugs for tonifying spleen and soothing nerves, and the traditional Chinese medicine composition has the effects of clearing heat and removing toxicity, killing insects, cooling blood and relieving pain and stabilizing mind by combining the. Meanwhile, the preparation method of the traditional Chinese medicine composition is simple and convenient to operate, water decoction of the gamboge water extract, the cyrtomium rhizome ethanol precipitate, the agrimony bud zymolyte and other raw medicinal materials is obtained step by step, effective components for treating histomoniasis are obtained, and a good effect of treating the histomoniasis is achieved.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating histomoniasis of turkeys, and a preparation method and application thereof.
Background
Histomoniasis (histomonasis), also known as cecal hepatitis or blackhead disease, is a protozoal disease caused by the parasite of turkey histomonads of the histomonads genus on the cecum and liver of birds, using chicken heterodera spinosa or earthworms as the transmission medium. Mainly invade the cecum and the liver, mostly occur to chicks and turkeys, and adult chickens can be infected, but the condition is mild; pheasants, peacocks, guinea fowl, quail, etc. can also sometimes be infected. The birds are infected by ingestion of feeds and drinking water contaminated with heterodera sinensis eggs, and may also be infected by ingestion of earthworms with heterodera sinensis eggs and larvae.
At present, chemical medicines such as metronidazole, metronidazole and robenidine are mostly adopted for treating histomoniasis, so that the treatment period is long, medicine residues are easy to generate, and great threat is caused to the chicken industry.
Disclosure of Invention
The invention aims to solve the technical problem of providing a traditional Chinese medicine composition which is reasonable in formula and obvious in effect and is used for treating the histomoniasis of turkeys in view of the defects of the prior art.
The invention also provides a preparation method of the traditional Chinese medicine composition for treating the histomoniasis of the turkey.
The invention also provides application of the traditional Chinese medicine composition in preparation of medicines for treating the turkey histomoniasis.
The technical problem to be solved by the invention is realized by the following technical scheme, and the traditional Chinese medicine composition for treating the turkey histomoniasis is characterized by comprising the following raw materials in parts by weight: 1-3 parts of gamboge, 5-10 parts of cyrtomium rhizome, 8-12 parts of agrimony bud, 5-10 parts of pepper and 10-15 parts of aloe.
In the technical scheme of the traditional Chinese medicine composition for treating the histomoniasis of the turkey, the traditional Chinese medicine composition also comprises the following raw materials in parts by weight: 4-11 parts of angelica dahurica, 5-15 parts of red peony root, 5-10 parts of subprostrate sophora, 8-12 parts of platycladi seed and 5-10 parts of cortex albiziae.
In the technical scheme of the traditional Chinese medicine composition for treating the histomoniasis of the turkey, the weight ratio of the raw materials is as follows: 1-2 parts of gamboge, 6-8 parts of cyrtomium rhizome, 8-10 parts of agrimony bud, 6-8 parts of pepper, 11-14 parts of aloe, 5-10 parts of angelica dahurica, 6-12 parts of red paeony root, 6-8 parts of subprostrate sophora, 8-10 parts of platycladi seed and 6-9 parts of cortex albiziae. Further preferably, the weight ratio of the raw materials is as follows: 1 part of gamboge, 8 parts of cyrtomium rhizome, 10 parts of agrimony bud, 6 parts of pepper, 14 parts of aloe, 6 parts of angelica dahurica, 6 parts of red peony root, 8 parts of subprostrate sophora, 10 parts of platycladi seed and 8 parts of cortex albiziae.
The invention also provides a preparation method of the traditional Chinese medicine composition in the technical scheme, which is characterized by comprising the following steps:
(1) freeze drying resina Garciniae, pulverizing, adding water, decocting under sealed condition, centrifuging, and concentrating the supernatant to obtain resina Garciniae water extract;
(2) cutting rhizoma Osmundae, adding water, decocting, filtering, collecting filtrate, adding ethanol into the filtrate for precipitating with ethanol, and collecting precipitate to obtain rhizoma Osmundae water extract and ethanol precipitate;
(3) cutting herba Agrimoniae bud, adding water, homogenizing to obtain homogenate, adding cellulase, performing enzymolysis, collecting upper layer enzymolysis liquid, and concentrating to obtain herba Agrimoniae bud zymolyte;
(4) cutting or pulverizing the rest materials respectively, mixing, adding water, soaking, decocting, filtering to obtain water decoction and residue, decocting the residue with water for 2-3 times, filtering, removing residue, and mixing water decoctions to obtain mixed water decoction;
(5) mixing the resina Garciniae water extract, rhizoma Osmundae water extract and ethanol precipitate, herba et Gemma Agrimoniae bud zymolyte and mixed decoction, and concentrating under reduced pressure to obtain concentrated solution with relative density of 1.1-1.2 at 65-75 deg.C.
In the preparation method of the traditional Chinese medicine composition, a further preferable technical scheme or technical characteristics is as follows:
1. in step (1): adding water into the gamboge powder according to the weight ratio of the raw materials to the water of 1: 5-10, decocting for 2-3h in a closed manner, centrifuging, and concentrating the supernatant to obtain the gamboge water extract.
2. In step (2): cutting rhizoma Osmundae into pieces, adding water at a weight ratio of 1: 8-15, decocting for 1-2h, filtering, collecting filtrate, adding 80-90% ethanol into the filtrate until the alcohol concentration reaches 60-75%, stirring well, standing, collecting precipitate to obtain rhizoma Osmundae water extract and alcohol precipitate.
3. In step (3): cutting the bud of the agrimony, adding water according to the weight ratio of 1: 2-6 for homogenate to obtain homogenate, adding cellulase accounting for 0.5-1.5% of the weight of the homogenate into the homogenate for enzymolysis for 3-5.5h, collecting the upper layer enzymolysis liquid, and concentrating to obtain the bud zymolyte of the agrimony.
4. In step (4): respectively cutting or crushing the rest raw material medicines, mixing, adding water with the weight of 8-10 times of the total weight of the mixed raw material medicines into the mixed raw material medicines, soaking for 2-5h at the water temperature of 35-45 ℃, boiling with strong fire until the mixture is boiled, maintaining for 20-30min after the mixture is boiled, decocting with slow fire for 0.5-1h, filtering to obtain water decoction and filter residues, decocting the filter residues with 3-6 times of water by weight for 2-3 times, 2-3h each time, filtering, discarding the filter residues, and combining the water decoctions to obtain mixed water decoction.
The traditional Chinese medicine composition disclosed by the invention is applied to preparation of a medicine for treating histomoniasis of turkeys.
The Chinese medicinal raw materials involved in the invention have the following medicinal properties:
gamboge: cool, sour, astringent and toxic; the functional indications are as follows: detumescence, counteracting toxic substance, stopping bleeding, killing parasites, removing putrefaction and treating ulcer, such as superficial infection, toxic swelling, ulcer, eczema, tinea, intractable tinea, swelling and pain due to traumatic injury, traumatic hemorrhage and scald.
Rhizoma Osmundae: cold, bitter and slightly toxic; the functional indications are as follows: clear heat and remove toxicity, stop bleeding, kill parasites. Can be used for treating common cold, fever, dysentery, damp heat, pyocutaneous disease, hematochezia, hematuria, menorrhagia, knife wound hemorrhage, ascariasis, parasitic worms, taeniasis, induced abortion, and puerperal hemorrhage.
And (3) gemma agrimoninae: slightly cold and bitter; it enters liver, small intestine and large intestine meridians; the functional indications are as follows: killing parasite, and can be used for treating tapeworm.
Chinese prickly ash: warm, pungent; spleen, stomach and kidney meridians entered; the functional indications are as follows: warming middle energizer to alleviate pain, killing parasites to relieve itching, and can be used for treating abdominal psychroalgia, emesis, diarrhea, abdominal pain due to parasitic infestation, ascariasis, and eczema and pruritus by external application.
Aloe vera: cold, bitter; it enters liver and large intestine meridians; the functional indications are as follows: purgation, liver heat clearing, parasites killing, constipation due to heat accumulation, headache due to liver fire, conjunctival congestion, convulsion, abdominal pain due to parasitic infestation, scabies, tinea, hemorrhoids and fistula, and croton toxin relieving.
Radix angelicae: warm, pungent; meridian tropism entering stomach, large intestine and lung meridian; the functional indications are as follows: it has effects in expelling pathogenic wind, removing dampness, inducing resuscitation, relieving pain, relieving swelling, and expelling pus, and can be used for treating common cold, headache, supraorbital bone pain, nasal obstruction, nasosinusitis, toothache, leucorrhea, and pyocutaneous disease with swelling and pain.
Red peony root: slightly cold and bitter; entering liver meridian; the functional indications are as follows: clearing heat and cooling blood, removing blood stasis and relieving pain, and can be used for treating warm-toxicity macula, hematemesis and epistaxis, conjunctival congestion and swelling pain, liver depression and hypochondriac pain, amenorrhea and dysmenorrhea, abdominal pain, traumatic injury, carbuncle, swelling and sore and ulcer.
Subprostrate sophora root: cold, bitter and toxic; entering lung and stomach meridians; the functional indications are as follows: clearing away heat and toxic materials, relieving swelling and pain, relieving constipation, treating acute pharyngolaryngitis, tonsillitis, dental swelling and pain, cough due to lung heat, jaundice due to damp-heat, carbuncle, furuncle, pyogenic infections and constipation.
Arborvitae seed: mild in nature and sweet; the heart, kidney and large intestine meridians entered; the functional indications are as follows: nourish heart and induce tranquilization, arrest sweating, moisten intestines and relieve constipation, and mainly treat palpitation due to fright, insomnia, amnesia, night sweat, constipation due to intestinal dryness.
Silktree albizzia bark: mild in nature and sweet; it enters liver, heart and spleen meridians; the functional indications are as follows: tranquilization, resolving stagnation, promoting blood circulation, relieving pain, reuniting bones and muscles, treating uneasiness, depression and insomnia, pulmonary abscess, bone and muscle injury, and pain due to anal fistula.
Compared with the prior art, the composition has reasonable formula compatibility, gamboge and cyrtomium rhizome are used as monarch drugs for clearing heat and removing toxicity and resisting bacteria and killing insects, the gemma agrimoninae and the pepper are used as ministerial drugs for expelling insects and killing insects, the aloe, the angelica dahurica, the red paeony root and the subprostrate sophora are used as assistants for clearing liver, cooling blood, diminishing swelling and relieving pain, the platycladi seed and the cortex albiziae are used as conductant drugs for tonifying spleen and soothing nerves, the effects of clearing heat and removing toxicity, killing insects, cooling blood and relieving pain and stabilizing mind are realized by combining the medicines, and. Meanwhile, the preparation method of the traditional Chinese medicine composition is simple and convenient to operate, water decoction of the gamboge water extract, the cyrtomium rhizome ethanol precipitate, the agrimony bud zymolyte and other raw medicinal materials is obtained step by step, effective components for treating histomoniasis are obtained, and a good effect of treating the histomoniasis is achieved.
The traditional Chinese medicine composition can be used for treating the histomoniasis of the turkeys, and has good treatment effect on other poultry suffering from the histomoniasis, such as pheasants, guinea fowls, quails and the like.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the present invention is not limited to these examples.
Embodiment 1, a traditional Chinese medicine composition for treating turkey histomoniasis comprises the following raw materials by weight: 1 part of gamboge, 5 parts of cyrtomium rhizome, 8 parts of agrimony bud, 5 parts of pepper and 10 parts of aloe.
Embodiment 2, a traditional Chinese medicine composition for treating turkey histomoniasis comprises the following raw materials by weight: 3 parts of gamboge, 10 parts of cyrtomium rhizome, 12 parts of agrimony bud, 10 parts of pepper and 15 parts of aloe.
The traditional Chinese medicine composition for treating histomoniasis of turkeys, which is described in embodiment 3, embodiment 1 or 2, further comprises the following raw materials in parts by weight: 4 parts of angelica dahurica, 5 parts of red peony root, 5 parts of subprostrate sophora, 8 parts of platycladi seed and 5 parts of cortex albiziae.
Example 4, the traditional Chinese medicine composition for treating histomoniasis of turkey described in example 1 or 2 further comprises the following raw materials by weight: 11 parts of angelica dahurica, 15 parts of red peony root, 10 parts of subprostrate sophora, 12 parts of platycladi seed and 10 parts of cortex albiziae.
In the traditional Chinese medicine composition for treating histomoniasis of turkeys, which is described in example 5, example 3, or example 4, the weight ratio of each raw material is as follows: 1 part of gamboge, 6 parts of cyrtomium rhizome, 8 parts of agrimony bud, 6 parts of pepper, 11 parts of aloe, 5 parts of angelica dahurica, 6 parts of red paeony root, 6 parts of subprostrate sophora, 8 parts of platycladi seed and 6 parts of cortex albiziae.
In the traditional Chinese medicine composition for treating histomoniasis of turkeys, which is described in example 6, example 3, or example 4, the weight ratio of each raw material is as follows: 2 parts of gamboge, 8 parts of cyrtomium rhizome, 10 parts of agrimony bud, 8 parts of pepper, 14 parts of aloe, 10 parts of angelica dahurica, 12 parts of red paeony root, 8 parts of subprostrate sophora, 10 parts of platycladi seed and 9 parts of cortex albiziae.
Example 7, the method of preparing the herbal composition of any of examples 1-6, comprising the steps of:
(1) freeze drying resina Garciniae, pulverizing, adding water, decocting under sealed condition, centrifuging, and concentrating the supernatant to obtain resina Garciniae water extract;
(2) cutting rhizoma Osmundae, adding water, decocting, filtering, collecting filtrate, adding ethanol into the filtrate for precipitating with ethanol, and collecting precipitate to obtain rhizoma Osmundae water extract and ethanol precipitate;
(3) cutting herba Agrimoniae bud, adding water, homogenizing to obtain homogenate, adding cellulase, performing enzymolysis, collecting upper layer enzymolysis liquid, and concentrating to obtain herba Agrimoniae bud zymolyte;
(4) cutting or pulverizing the rest materials respectively, mixing, adding water, soaking, decocting, filtering to obtain water decoction and residue, decocting the residue with water for 2-3 times, filtering, removing residue, and mixing water decoctions to obtain mixed water decoction;
(5) mixing the resina Garciniae water extract, rhizoma Osmundae water extract and ethanol precipitate, herba et Gemma Agrimoniae bud zymolyte and mixed water decoction, and concentrating under reduced pressure to obtain concentrated solution with relative density of 1.1 at 65 deg.C.
Example 8, the method of preparing the herbal composition of any of examples 1-6, comprising the steps of:
(1) freeze drying resina Garciniae, pulverizing, adding water, decocting under sealed condition, centrifuging, and concentrating the supernatant to obtain resina Garciniae water extract;
(2) cutting rhizoma Osmundae, adding water, decocting, filtering, collecting filtrate, adding ethanol into the filtrate for precipitating with ethanol, and collecting precipitate to obtain rhizoma Osmundae water extract and ethanol precipitate;
(3) cutting herba Agrimoniae bud, adding water, homogenizing to obtain homogenate, adding cellulase, performing enzymolysis, collecting upper layer enzymolysis liquid, and concentrating to obtain herba Agrimoniae bud zymolyte;
(4) cutting or pulverizing the rest materials respectively, mixing, adding water, soaking, decocting, filtering to obtain water decoction and residue, decocting the residue with water for 2-3 times, filtering, removing residue, and mixing water decoctions to obtain mixed water decoction;
(5) mixing the resina Garciniae water extract, rhizoma Osmundae water extract and ethanol precipitate, herba et Gemma Agrimoniae bud zymolyte and mixed water decoction, and concentrating under reduced pressure to obtain concentrated solution with relative density of 1.2 at 75 deg.C.
Example 9, example 7 or 8, adding water to the gamboge powder according to the weight ratio of 1: 5, decocting for 2h in a closed environment, centrifuging, and concentrating the supernatant to obtain the aqueous extract of gamboge.
Example 10, example 7 or 8, adding water to the gamboge powder according to a weight ratio of 1:12, decocting for 3h in a closed environment, centrifuging, and concentrating the supernatant to obtain the aqueous extract of gamboge.
Example 11, example 7 or 8, wherein in the step (2) of the preparation method, the cyrtomium fortunei is cut into pieces, water is added into the pieces according to the weight ratio of the materials to the water of 1:8, the pieces are decocted for 1 hour, the filtering is carried out, the filtrate is collected, 80% ethanol is added into the filtrate until the alcohol concentration reaches 60%, the mixture is fully and uniformly stirred, the standing is carried out, and the precipitate is collected, so that the cyrtomium fortunei water alcohol precipitate is obtained.
Example 12, example 7 or 8, wherein in the step (2) of the preparation method, the cyrtomium fortunei is cut into pieces, water is added into the pieces according to the weight ratio of the materials to the water of 1: 15, the pieces are decocted for 2 hours, the filtering is carried out, the filtrate is collected, 90% ethanol is added into the filtrate until the alcohol concentration reaches 75%, the mixture is fully and uniformly stirred, the standing is carried out, and the precipitate is collected, so that the cyrtomium fortunei water alcohol precipitate is obtained.
Example 13, example 7 or 8, wherein in step (3) of the preparation method, the agrimony bud is cut into pieces, water is added according to the weight ratio of 1: 2 to homogenize the pieces, so as to obtain homogenate, cellulase accounting for 0.5 percent of the weight of the homogenate is added into the homogenate to carry out enzymolysis for 3 hours, and upper layer enzymolysis liquid is collected and concentrated, so as to obtain the agrimony bud zymolyte.
Example 14, example 7 or 8, wherein in step (3) of the preparation method, the agrimony bud is cut into pieces, water is added according to the weight ratio of 1: 6 to homogenize the pieces, so as to obtain homogenate, cellulase accounting for 1.5 percent of the weight of the homogenate is added into the homogenate to carry out enzymolysis for 5.5 hours, and upper layer enzymolysis liquid is collected and concentrated, so as to obtain the agrimony bud zymolyte.
In the step (4) of the preparation method described in embodiment 15, embodiment 7, or 8, the remaining raw material drugs are respectively cut or crushed, mixed, water with an amount of 8 times of the total weight of the mixed raw material drugs is added to the mixed raw material drugs, the water temperature is 35 ℃, the mixed raw material drugs are soaked for 2 hours, the mixed raw material drugs are boiled with strong fire until boiling, the boiling is maintained for 20 minutes, the mixed raw material drugs are boiled with slow fire for 0.5 hour, filtered to obtain a water decoction and filter residues, the filter residues are boiled with 3 times of the water for 2 times, 2 hours each time, filtered, the filter residues are discarded, and the water decoctions are combined to obtain a mixed water decoction.
In the step (4) of the preparation method described in embodiment 16, embodiment 7, or 8, the remaining raw material drugs are respectively cut or crushed, mixed, water 10 times the total weight of the mixed raw material drugs is added to the mixed raw material drugs, the water temperature is 45 ℃, the mixed raw material drugs are soaked for 5 hours, the mixed raw material drugs are boiled with strong fire until boiling, the boiling is maintained for 30min, the mixed raw material drugs are boiled with slow fire for 1 hour, and filtered to obtain water decoction and filter residues, the filter residues are boiled with 6 times of water for 3 times, 3 hours each time, and filtered to remove the filter residues, and the water decoction of each time is combined to obtain the mixed water decoction.
Embodiment 17, a traditional Chinese medicine composition for treating turkey histomoniasis comprises the following raw materials by weight: 1.5 of gamboge, 10 of cyrtomium rhizome, 8 of agrimony bud, 10 of pepper and 10 of aloe.
The preparation method of the traditional Chinese medicine composition comprises the following steps:
(1) freeze drying resina Garciniae, pulverizing to 150 mesh, adding water into resina Garciniae powder at a weight ratio of 1:8, decocting for 3 hr under sealed condition, centrifuging, and concentrating the supernatant to obtain resina Garciniae water extract;
(2) cutting rhizoma Osmundae into pieces, adding water at a weight ratio of 1:12, decocting for 2h, filtering, collecting filtrate, adding 85% ethanol into the filtrate until the alcohol concentration reaches 60%, stirring thoroughly, standing, collecting precipitate to obtain rhizoma Osmundae water extract and alcohol precipitate;
(3) cutting the bud of the agrimony, adding water according to the weight ratio of 1:4 for homogenate to obtain homogenate, adding cellulase accounting for 1% of the weight of the homogenate into the homogenate for enzymolysis for 4 hours, collecting the upper layer enzymolysis liquid, and concentrating to obtain an enzymolysis product of the bud of the agrimony;
(4) respectively cutting or crushing the rest raw material medicines, mixing, adding water with the weight of 8 times of the total weight of the mixed raw material medicines into the mixed raw material medicines, soaking for 4h at the water temperature of 40 ℃, boiling with strong fire until the mixture is boiled, maintaining for 25min after the mixture is boiled, then decocting with slow fire for 0.8h, filtering to obtain water decoction and filter residue, decocting the filter residue with water with the weight of 4 times for 2-3 times, 2h each time, filtering, discarding the filter residue, and combining the water decoction to obtain mixed water decoction;
(5) uniformly mixing the gamboge water extract, the cyrtomium rhizome water extract and alcohol precipitate, the agrimony bud zymolyte and the mixed water decoction, and concentrating under reduced pressure to obtain a concentrated solution with the relative density of 1.15 at 70 ℃, namely the traditional Chinese medicine composition.
Embodiment 18, a traditional Chinese medicine composition for treating turkey histomoniasis comprises the following raw materials by weight: 1 part of gamboge, 8 parts of cyrtomium rhizome, 10 parts of agrimony bud, 6 parts of pepper, 14 parts of aloe, 6 parts of angelica dahurica, 6 parts of red peony root, 8 parts of subprostrate sophora, 10 parts of platycladi seed and 8 parts of cortex albiziae;
the preparation method of the Chinese medicinal composition is the same as that of example 17.
When the traditional Chinese medicine composition is used, the traditional Chinese medicine composition is mixed into chicken feed according to 0.02-0.05% of the weight of turkeys for eating by sick chickens, or 1.5-2.0g of the traditional Chinese medicine composition is added into 1 ton of drinking water of the chickens, and the traditional Chinese medicine composition is taken orally by drinking water 2 times a day, once in the morning and evening, and is continuously used for 2-3 days.
Example 18, test example.
1. Test animal
A certain turkey farm in east sea county of Hongyun harbor city can be comprehensively diagnosed according to clinical symptoms, pathological anatomy and microscopic examination, and turkeys diagnosed with histomoniasis are used as test chickens.
The sick chicken has dull appearance, depressed spirit, reduced or stopped food intake, loose feathers, drooping wings, water-affected walking, bluish purple head skin, fear of cold crowding, bloody feces or yellow green. Serious cecal hemorrhage can be seen in pathological dissection, intestinal contents are blood clots, the wall thickness of the cecum is thick and large, and the cross-section of the cecum is mostly concentric and layered; the liver is swollen, the surface has round or irregular sunken dead areas, the color is light yellow or light green, the edges are raised, and the ulcer range is different. Laboratory examination, sterile operation to take fresh intestinal contents of sick chicken, diluting with normal saline, and making into hanging drop specimen to show the original insect body in reciprocating pendulum motion.
2. Test method
The traditional Chinese medicine composition of the invention comprises 1: the traditional Chinese medicine composition prepared in the embodiment 17 of the invention is mixed with feed according to 0.02% of the weight of the turkey and continuously used for 3 days;
the traditional Chinese medicine composition of the invention comprises the following components: the traditional Chinese medicine composition prepared in the embodiment 17 of the invention is mixed with feed according to 0.05 percent of the weight of the turkey and continuously used for 3 days;
the traditional Chinese medicine composition of the invention comprises 3: by adopting the traditional Chinese medicine composition prepared in the embodiment 18, 1.5g of the traditional Chinese medicine composition is added into 1 ton of drinking water of chickens, and the traditional Chinese medicine composition is taken orally by drinking water for 3 days continuously;
the traditional Chinese medicine composition of the invention has the following components: 2.0g of the traditional Chinese medicine composition prepared in the embodiment 18 of the invention is added into every 1 ton of drinking water of chicken, and the traditional Chinese medicine composition is taken orally by drinking water for 3 days continuously;
control drug group: metronidazole (manufactured by Guangdong south China pharmaceutical industry group Co., Ltd., lot number 20161201), used according to the instructions, continuously administered for 3 days;
blank control group: it is not used.
The test turkey has the feeding amount of slightly excess feed in the feeding trough after being full, and water is freely drunk.
4. Observation of effects
4.1 cure rate
After continuous administration for 3 days, clinical symptoms disappear, no obvious pathological changes are caused by pathological anatomy, and spirit, food intake and drinking water are recovered to be normal.
4.2 invalidation
After continuous administration for 3 days, the clinical symptoms continue to worsen, the pathological anatomy has obvious pathological changes, and the spirit, food intake and drinking water are treated before.
5. Test results
Based on the above observations, cure rate, inefficiency and mortality were calculated, and the test results are shown in table 1.
TABLE 1 animal test results
| Group of | Percent cure rate/%) | Non efficiency/%) | Mortality rate/%) |
| The invention relates to a traditional Chinese medicine combination group 1 | 88.4 | 7.2 | 4.4 |
| The invention relates to a traditional Chinese medicine combination group 2 | 90.5 | 5.9 | 3.6 |
| The invention relates to a traditional Chinese medicine combination group 3 | 92.2 | 4.8 | 3.0 |
| The invention relates to a traditional Chinese medicine combination group 4 | 93.7 | 3.5 | 2.8 |
| Control drug group | 81.4 | 7.9 | 10.7 |
| Blank control group | - | - | 18.3 |
The experimental results show that compared with the control drug group, the traditional Chinese medicine composition has more remarkable treatment effect. Meanwhile, 5 medicines of radix angelicae, radix paeoniae rubra, subprostrate sophora, platycladi seed and cortex albiziae are added in the formula to play a role in synergy, and the prepared traditional Chinese medicine composition has a better effect of treating the histomoniasis of turkeys.
Claims (1)
1. A traditional Chinese medicine composition for treating histomoniasis of turkeys is characterized by comprising the following raw materials in parts by weight:
1 part of gamboge, 8 parts of cyrtomium rhizome, 10 parts of agrimony bud, 6 parts of pepper, 14 parts of aloe, 6 parts of angelica dahurica, 6 parts of red peony root, 8 parts of subprostrate sophora, 10 parts of platycladi seed and 8 parts of cortex albiziae;
the preparation method of the traditional Chinese medicine composition comprises the following steps:
(1) freeze drying resina Garciniae, pulverizing to 150 mesh, adding water into resina Garciniae powder at a weight ratio of 1:8, decocting for 3 hr under sealed condition, centrifuging, and concentrating the supernatant to obtain resina Garciniae water extract;
(2) cutting rhizoma Osmundae into pieces, adding water according to the weight ratio of 1:12, decocting for 2h, filtering, collecting filtrate, adding 85% ethanol into the filtrate until the alcohol concentration reaches 60%, stirring thoroughly, standing, collecting precipitate to obtain rhizoma Osmundae water extract and alcohol precipitate;
(3) cutting the bud of the agrimony, adding water according to the weight ratio of 1:4 of the water to homogenate to obtain homogenate, adding cellulase accounting for 1% of the weight of the homogenate to perform enzymolysis for 4 hours, collecting upper layer enzymolysis liquid, and concentrating to obtain an enzymolysis product of the bud of the agrimony;
(4) respectively cutting or crushing the rest raw material medicines, mixing, adding water with the weight of 8 times of the total weight of the mixed raw material medicines into the mixed raw material medicines, soaking for 4h at the water temperature of 40 ℃, boiling with strong fire until the mixture is boiled, maintaining for 25min after the mixture is boiled, then decocting with slow fire for 0.8h, filtering to obtain water decoction and filter residue, decocting the filter residue with water with the weight of 4 times for 2-3 times, 2h each time, filtering, discarding the filter residue, and combining the water decoction to obtain mixed water decoction;
(5) uniformly mixing the gamboge water extract, the cyrtomium rhizome water extract and alcohol precipitate, the agrimony bud zymolyte and the mixed water decoction, and concentrating under reduced pressure to obtain a concentrated solution with the relative density of 1.15 at 70 ℃, namely the traditional Chinese medicine composition.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5061491A (en) * | 1988-12-30 | 1991-10-29 | Deryabin Alexandr M | Medicinal agent and method for treatment of mastitis in animals and humans |
| CN104161853A (en) * | 2013-08-30 | 2014-11-26 | 郑州后羿制药有限公司 | Traditional Chinese medicine composition for treating livestock and poultry hepatitis and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5061491A (en) * | 1988-12-30 | 1991-10-29 | Deryabin Alexandr M | Medicinal agent and method for treatment of mastitis in animals and humans |
| CN104161853A (en) * | 2013-08-30 | 2014-11-26 | 郑州后羿制药有限公司 | Traditional Chinese medicine composition for treating livestock and poultry hepatitis and preparation method thereof |
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