CN107988102A - A kind of soil improvement and plant health activity complex micro organism fungicide - Google Patents
A kind of soil improvement and plant health activity complex micro organism fungicide Download PDFInfo
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- CN107988102A CN107988102A CN201711302722.2A CN201711302722A CN107988102A CN 107988102 A CN107988102 A CN 107988102A CN 201711302722 A CN201711302722 A CN 201711302722A CN 107988102 A CN107988102 A CN 107988102A
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- micro organism
- soil improvement
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- organism fungicide
- plant health
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/002—Fertilisers made from animal corpses, or parts thereof from fish or from fish-wastes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention discloses a kind of soil improvement and plant health active complex micro organism fungicide and preparation method thereof, the raw material of following mass parts are included：12 ~ 15g of cane molasses, 0.8 ~ 2.0g of milt cake, 10 ~ 15g of starch；Gram positive actinomycetes, decomposer, open root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, root circle protection bacterium seed liquor each 0.6 ~ 1.5%；Surplus is pure water；Separately it is related to a kind of soil improvement and the preparation method of the active complex micro organism fungicide of plant health, the soil improvement of the present invention and plant health activity complex micro organism fungicide, with with bacterium gram worm, with the antibacterial mechanism of action of bacterium, eliminate root-knot nematode adult, larva and worm's ovum is decomposed, effectively prevent root rot, virosis, bacterial wilt, damping-off, stem rot, dead seedling, rotten, the soil-borne disease such as droop.
Description
Technical field
The present invention relates to technical field of biological fertilizer, more particularly to a kind of soil improvement and plant health activity it is compound micro-
Bacteria agent and preparation method thereof.
Background technology
Ferment bacterium technology, is a bioengineering, its product can produce the effective microorganisms of a variety of catalytic decomposition acid
Body, the biotechnology as evolution type crystallize, and new hope is brought for agricultural sustainable development.
Early in eighties of last century the forties, Japanese microbiologist start a novel agricultural new and high technology of research-
Ferment bacterium technology, enters the application stage in the late nineteen eighties, is applied to plantation by more than 20 a countries and regions of the world
The fields such as industry, aquaculture, environmental protection, body-care, and achieve huge economic benefit and social benefit.From 1997 from Japan
This new and high technology has been introduced, and has carried out experiment, demonstration and has promoted, has achieved highly significant effect.
In the prior art, the fertilising for plant mainly uses traditional farm manure and chemical fertilizer, and mainly with chemical fertilizer
Based on, if application publication number is CN1443731A, publication date discloses in September, 2003 Chinese invention patent application of 24 days
A kind of Plant leaf fertilizer, is substantially made of the compound of following ratio of weight and number：Ethylenediaminetetraacetic acid zinc, 10-14；Have
The sylvite of machine acid, 32-38；Potassium sulfate, 40-50；Ammonium molybdate, 0.8-1.3.Wherein, the sylvite of the organic acid can be tricarboxylic
The sylvite of acid compounds, such as potassium citrate or desoxalic acid potassium.Foliar fertilizer of the present invention is solid dosage forms, volume
Small, light-weight, convenience in transport is with strong points, and significant effect, cost is low, is easy to be received by vast farmers.
But although chemical fertilizer is convenient, long-time service can cause the soil structure of soil to be destroyed, the change contained in chemical fertilizer
The material such as harmful ion such as dispersant, surfactant is learned, qualitative change can be produced to crops, influence the nutritional ingredient of crops,
Meanwhile chemical composition can suppress or kill endophytic profitable strain, destroy the microbial balance in body, influence plant
Normal growth, thus cause plant disease-resistant ability to reduce, the medicament residue of even chemical fertilizer cause the health of people by
Serious infringement.
The content of the invention
For above-mentioned problem, the present invention provide a kind of soil improvement and plant health complex micro organism fungicide and
Its preparation method, complex micro organism fungicide provided by the invention, strain containing multiple beneficial, can be effective with bacterium gram bacterium, with bacterium gram worm
Eliminate root-knot nematode and worm's ovum in soil；The growth and breeding of soil-borne disease source bacterium can effectively be suppressed；What is wherein contained opens root fungus and can promote
New root is sent out, promotes growth；Chesson can also be reached, improved soil nodule structure, improves the gas permeability of soil, water penetration, improves
Fertilizer conservation, water holding capacity；Promote plant growing way healthy and strong, strengthen the inverse anti-ability such as disease-resistant, freeze proof, anti-continuous cropping, drought resisting, waterlogging-resistant.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of active complex micro organism fungicide of soil improvement, includes the raw material of following mass parts：
12 ~ 15g of cane molasses, 0.8 ~ 2.0g of milt cake, 10 ~ 15g of starch；
Gram positive actinomycetes, decomposer, open root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, root circle protection bacterium seed liquor
Each 0.8 ~ 1.5%；
Surplus is pure water.
Preferably, the Gram positive actinomycetes, decomposer, open root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, root
Circle protection bacterium seed liquor is respectively 1%.
A kind of soil improvement preparation method of active complex micro organism fungicide, comprises the following steps：
A, weigh cane molasses, milt cake, starch in proportion to be placed in the fermenter of sterilization treatment, then in fermenter
Pure water is injected, is stirred for uniformly and heats to 120 DEG C, be cooled to 30 ~ 32 DEG C；
B, Gram positive actinomycetes, decomposer are added, opens root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, the protection of root circle
Bacterium seed liquor simultaneously stirs evenly, and stirs 1 ~ 3 time daily, and fermenter inflated oxygenation, daily 1 ~ 2 time, ferments 25 ~ 30 days, sees
Bacterium material fermentation process is examined, adjustment pH value is maintained at 5 ~ 5.5；
C, examine：Examined by quality standard；
D, metering packing：Finished product is classified by measure and is packed；
Preferably, in the step a, the pure water must be first through activated carbon zeolite filtration.
Preferably, in the step c, aerated conduit is provided with the fermenter.
Preferably, the preparation process of described circle protection bacterium seed liquor includes：Prepare culture medium：Include using by every 100ML
In quantitative distilled water and following component：5 ~ 10g of egg, 6 ~ 8g of milk, 5 ~ 7g of dregs of beans, 4 ~ 6g of rice bran, 5 ~ 7g of seawood meal
Stock；Each component is mixed, adjusts PH to 7.1 ~ 7.5 after stirring and dissolving, in 120 DEG C of high-temperature sterilizations 15 minutes, waits culture medium
Temperature is sub-packed in after being cooled to less than 60 DEG C in sterilizing flask；Root circle protection bacterium is inoculated in the culture medium.
Preferably, the egg 8g, milk 6g, dregs of beans 7g, rice bran 6g, seawood meal 5g.
Compared with prior art, soil improvement of the invention is had the beneficial effect that with active complex micro organism fungicide：1）Containing more
Kind of probiotics, with bacterium it is antibacterial, with bacterium gram worm, can effectively eliminate root-knot nematode and worm's ovum in soil；Soil-borne disease can effectively be suppressed
The growth and breeding of source bacterium；2）What is contained opens root fungus and can inspire new root, promotes growth；Chesson, improvement soil group can also be reached
Kernel structure, improves the gas permeability of soil, water penetration, improves fertilizer conservation, water holding capacity, promotes plant growing way healthy and strong, enhancing is disease-resistant, anti-
The inverse anti-ability such as jelly, anti-continuous cropping, drought resisting, waterlogging-resistant；3）Also containing nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium solubilizing bacteria, chemical fertilizer application can be greatly reduced
Amount, improves farm manure or organic fertilizer efficiency, farm manure is become efficient biologic-organic fertilizer.
Brief description of the drawings
Fig. 1 is soil improvement and the active complex micro organism fungicide production technological process of plant health of the present invention.
Embodiment
With reference to embodiment, the invention will be further described.
Strain used：
Gram positive actinomycetes, active quantities：7.9 × 10^7/ml；
Decomposer, active quantities：7.9 × 10^7/ml；
Open root fungus, active quantities：4.6 × 10^7/ml；
VA VA Mycorrhizal Fungis, active quantities：4.6 × 10^7/ml；
Photosynthetic bacteria, active quantities：3.5 × 10^7/ml；
Bacillus, active quantities：3.5 × 10^7/ml；
Root circle protects bacterium, active quantities：2.6 × 10^7/ml；
Embodiment 1
1st, the activation medium of Gram positive actinomycetes is prepared：
Raw material：Egg 8g, milk 7g, fish meal 4g, yeast extract 3g, glucose 15g, sodium acetate 4g, distilled water
100ml；
Preparation method：Each component is mixed in proportion, adjusts PH to 7.1 ~ 7.5, in 120 DEG C of high-temperature sterilizations after stirring and dissolving
15 minutes, etc. culture medium temperature be cooled to after less than 60 DEG C be sub-packed in sterilizing flask in, Gram positive actinomycetes are inoculated with
In the culture medium, every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, up to Gram positive actinomycetes seed liquor.
2nd, prepare decomposer and be inoculated in activation medium：
Raw material：Egg 6g, milk 10g, dregs of beans 5g, seawood meal 5g, manganese sulfate 0.03g, potassium phosphate 1g,
Distilled water 100ml；
Preparation method：Proportionally above-mentioned each component is mixed, after stirring and dissolving, adjustment pH value is to 6.0 ~ 6.5, at 120 DEG C
Sterilize 15 minutes under high temperature；Then, culture medium temperature is cooled to less than 60 DEG C, adds the candy by membrane filtration, sterilization
Class, it is 1.2% or less to make carbohydrate ultimate density in culture medium, is distributed into sterilizing test tubes, decomposer is inoculated in the training
Support in base, every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, cultivated 25 days below 30 DEG C, be decomposer seed liquor.
3rd, preparation opens root fungus and is inoculated in activation medium：
Raw material：Egg 10g, milk 8g, fish meal 5g, molasses 6g, rice bran 6g, distilled water 100ml；
Preparation method：Proportionally above-mentioned each component is mixed, after stirring and dissolving, PH to 6.0 ~ 6.5 is adjusted, in 120 DEG C of high temperature
Lower sterilizing 15 minutes；Then culture medium temperature is cooled to less than 60 DEG C, adds the carbohydrate by membrane filtration, sterilization, make training
It is 1.2% or less to support carbohydrate ultimate density in base, is distributed into sterilizing test tubes, will open root fungus and be inoculated in the culture medium,
Every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, cultivated 25 days below 30 DEG C, as open root fungus seed liquor.
4th, VA VA Mycorrhizal Fungis are prepared and is inoculated in activation medium：
Raw material：Egg 10g, milk 8g, fish meal 6g, molasses 8g, rice bran 5g, yeast extract 5g, distilled water 100ml,
Preparation method：Proportionally mentioned component is mixed, after stirring and dissolving, PH to 6.5 ~ 7.0 is adjusted, under 120 DEG C of high temperature
Sterilizing 15 minutes；Then, culture medium temperature is cooled to less than 60 DEG C, adds the carbohydrate by membrane filtration, sterilization, make culture
Carbohydrate ultimate density is 1.2% or less in base, is distributed into sterilizing test tubes, VA VA Mycorrhizal Fungis are inoculated in the culture medium
In, every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, cultivated 25 days below 30 DEG C, be VA VA Mycorrhizal Fungi seed liquors.
5th, photosynthetic bacteria, bacillus, root circle protection bacterium are inoculated in activation medium：
Raw material：Egg 8g, milk 6g, dregs of beans 7g, rice bran 6g, seawood meal 5g, distilled water 100ml,
Preparation method：After above-mentioned material stirring and dissolving, PH to 6.5 ~ 7.0 is adjusted, is sterilized 15 minutes under 120 DEG C of high temperature；
Then, culture medium temperature is cooled to less than 60 DEG C, adds the carbohydrate by membrane filtration, sterilization, make in culture medium carbohydrate most
Final concentration of 1.2% or less, it is distributed into sterilizing test tubes, photosynthetic bacteria, bacillus, root circle protection bacterium is inoculated in described
In culture medium, every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, cultivated 25 days below 30 DEG C, be photosynthetic bacteria, gemma
Bacillus, root circle protection bacterium seed liquor.
6th, complex micro organism fungicide is prepared：
A, it is placed in by cane molasses 10%, milt cake 0.5%, starch 10% in the fermenter of sterilization treatment, then in fermenter
Pure water of the middle injection after activated carbon zeolite filtration, is stirred for uniformly and heats to 120 DEG C；It is cooled to 30 ~ 32 DEG C；
B, Gram positive actinomycetes, decomposer are added, opens root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, the protection of root circle
Bacterium seed liquor each 1% simultaneously stirs evenly, and stirring 3 times, are provided with aerated conduit daily in fermenter, fermenter is inflated oxygenation, often
It 2 times, ferment 25 days, observe bacterium material fermentation process, adjustment pH value is maintained at 5 ~ 5.5；
C, examine：Examined by quality standard；
D, metering packing：Finished product is classified by measure and is packed；
Embodiment 2
1st, the activation medium of Gram positive actinomycetes：
Raw material：Egg 10g, milk 8g, fish meal 3g, yeast extract 4g, glucose 12g, sodium acetate 3g, distilled water
100ml；
Preparation method：Each component is mixed in proportion, adjusts PH to 7.1 ~ 7.5, in 130 DEG C of high-temperature sterilizations after stirring and dissolving
15 minutes, etc. culture medium temperature be cooled to after less than 60 DEG C be sub-packed in sterilizing flask in, Gram positive actinomycetes are inoculated with
In the culture medium, every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, up to Gram positive actinomycetes seed liquor.
2nd, decomposer is inoculated in activation medium：
Raw material：Egg 8g, milk 8g, dregs of beans 4g, seawood meal 5g, manganese sulfate 0.05g, potassium phosphate 1.2g, distilled water
100ml；
Preparation method：After above-mentioned material stirring and dissolving, adjustment pH value sterilizes 15 points to 6.0 ~ 6.5 under 130 DEG C of high temperature
Clock；Then, culture medium temperature is cooled to less than 60 DEG C, adds the carbohydrate by membrane filtration, sterilization, make candy in culture medium
Class ultimate density is 1.2% or less, is distributed into sterilizing test tubes, decomposer is inoculated in the culture medium, every gram of training
It is 1.4 × 10 to support base inoculating strain quantity6, cultivated 28 days below 30 DEG C, be decomposer seed liquor.
3rd, open root fungus and be inoculated in activation medium：
Raw material：Egg 10g, milk 10g, fish meal 6g, molasses 4g, rice bran 6g, distilled water 100ml,
Preparation method：After above-mentioned material stirring and dissolving, PH to 6.0 ~ 6.5 is adjusted, is sterilized 15 minutes under 130 DEG C of high temperature；
Then, culture medium temperature is cooled to less than 60 DEG C, adds the carbohydrate by membrane filtration, sterilization, make in culture medium carbohydrate most
Final concentration of 1.2% or less, it is distributed into sterilizing test tubes, root fungus will be opened and be inoculated in the culture medium, every gram of culture medium
Inoculating strain quantity is 1.4 × 106, cultivated 28 days below 30 DEG C, as open root fungus seed liquor.
4th, VA VA Mycorrhizal Fungis are inoculated in activation medium：
Raw material：Egg 8g, milk 8g, fish meal 4g, molasses 8g, rice bran 5g, yeast extract 5g,
Distilled water 100ml,
Preparation method：After above-mentioned material stirring and dissolving, PH to 6.5 ~ 7.0 is adjusted, is sterilized 15 minutes under 130 DEG C of high temperature；
Then, culture medium temperature is cooled to less than 60 DEG C, adds the carbohydrate by membrane filtration, sterilization, make in culture medium carbohydrate most
Final concentration of 1.2% or less, it is distributed into sterilizing test tubes, VA VA Mycorrhizal Fungis is inoculated in the culture medium, every gram of culture
Base inoculating strain quantity is 1.4 × 106, cultivated 28 days below 30 DEG C, be VA VA Mycorrhizal Fungi seed liquors.
5th, photosynthetic bacteria, bacillus, root circle protection bacterium are inoculated in activation medium：
Raw material：Egg 10g, milk 8g, dregs of beans 7g, rice bran 6g, seawood meal 5g, distilled water 100ml；
Preparation method：After above-mentioned material stirring and dissolving, PH to 6.5 ~ 7.0 is adjusted, is sterilized 15 minutes under 130 DEG C of high temperature；
Then, culture medium temperature is cooled to less than 60 DEG C, adds the carbohydrate by membrane filtration, sterilization, make in culture medium carbohydrate most
Final concentration of 1.2% or less, it is distributed into sterilizing test tubes, photosynthetic bacteria, bacillus, root circle protection bacterium is inoculated in institute
State in culture medium, every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, cultivated 28 days below 30 DEG C, be photosynthetic bacteria, bud
Spore bacillus, root circle protection bacterium seed liquor.
5th, active complex micro organism fungicide is prepared：
A. it is placed in the fermenter of sterilization treatment by cane molasses 10%, milt cake 0.5%, starch 10%, is then sending out
Pure water is injected in ferment bucket, is stirred for uniformly and heats to 120 DEG C,；It is cooled to 30 ~ 32 DEG C；
B. Gram positive actinomycetes, decomposer are added, opens root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, the protection of root circle
Bacterium seed liquor each 1% simultaneously stirs evenly, daily stirring 2 times, and fermenter inflated oxygenation, daily 1 time, ferments 28 days, sees
Bacterium material fermentation process is examined, adjustment pH value is maintained at 5 ~ 5.5；
C. examine：Examined by quality standard；
D. metering packing：Finished product is classified by measure and is packed；
In the step a, the pure water must be first through activated carbon zeolite filtration；
In the step c, the fermenter must be provided with aerated conduit.
Product characteristics：This product is liquid.
Product feature：Eliminate root-knot nematode and worm's ovum in soil：Chitin and protein are root-knot nematode body wall and chorion
Main component, VA Mycorrhizal Fungi that semi-finished product class contains, root circle protection bacterium and actinomyces can penetrate chitin and decomposing protein, make root
The rapid atrophy dehydration of tie lines worm adult, larva, so it is dead, and worm's ovum resolution, no longer recurs.Prevent root decomposing disease, stem rot disease,
The soil-borne diseases such as bacterial wilt, droop；Inspire new root：New root can be inspired by opening root fungus, grow root mass, strong sprout, strong, promote life
It is long；Activating soil:Chesson, improved soil nodule structure, improves the gas permeability of soil, water penetration, improves fertilizer conservation, water holding capacity
Promote plant growing way healthy and strong, strengthen the anti-adversity ability such as disease-resistant, freeze proof, anti-continuous cropping, drought resisting, waterlogging-resistant.
Usage and dosage：
1st, the crop compared with dense planting such as booth vegetable, outdoor vegetables, industrial crops：It is de- once with water punching with 2 liters of this product per acre,
The plant being in a bad way is watered 30 times of dilution, every plant of 200 milliliters of root of pouring with this product.
2nd, the application method of fruit tree：Prevention is watered 100 times of dilution with this product, and little tree every pours 200 milliliters of root,
Uncle every pours 500-1000 milliliters；Treatment is watered 30 times of dilution with this product, and little tree every pours 200 milliliters of root, greatly
Set every and pour root 500-1000 milliliters.
Claims (7)
1. a kind of soil improvement and plant health activity complex micro organism fungicide, it is characterised in that include following mass parts
Raw material：
12~15g of cane molasses, 0.8~2.0g of milt cake, 10~15g of starch；
Gram positive actinomycetes, decomposer, open root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, root circle protection bacterium seed liquor
Each 0.8~1.5%；
Surplus is pure water.
2. a kind of soil improvement according to claim 1 and the compound complex micro organism fungicide of plant health activity, it is special
Sign is：The Gram positive actinomycetes, decomposer, open root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, root circle protection bacterium
Seed liquor is respectively 1%.
A kind of 3. preparation side of the soil improvement according to claim 1 with plant health with active complex micro organism fungicide
Method, it is characterised in that comprise the following steps：
A, weigh cane molasses, milt cake, starch in proportion to be placed in the fermenter of sterilization treatment, then in fermenter
Pure water is injected, is stirred for uniformly and heats to 120 DEG C, be cooled to 30~32 DEG C；
B, Gram positive actinomycetes, decomposer are added, opens root fungus, VA VA Mycorrhizal Fungis, photosynthetic bacteria, bacillus, the protection of root circle
Bacterium seed liquor simultaneously stirs evenly, and stirs 1~3 time daily, and fermenter inflated oxygenation, daily 1~2 time, ferments 25~30 days, sees
Bacterium material fermentation process is examined, adjustment pH value is maintained at 5~5.5；
C, examine：Examined by quality standard；
D, metering packing：Finished product is classified by measure and is packed.
A kind of 4. preparation side of the soil improvement according to claim 3 with plant health with active complex micro organism fungicide
Method, it is characterised in that in the step a, the pure water must be first through activated carbon zeolite filtration.
A kind of 5. preparation side of the soil improvement according to claim 3 with plant health with active complex micro organism fungicide
Method, it is characterised in that in the step c, aerated conduit is provided with the fermenter.
A kind of 6. preparation side of the soil improvement according to claim 3 with plant health with active complex micro organism fungicide
Method, it is characterised in that the preparation process of described circle protection bacterium seed liquor includes：Prepare culture medium：Include being used for by every 100ML
Quantitative distilled water and following component：5~10g of egg, 6~8g of milk, 5~7g of dregs of beans, 4~6g of rice bran, 5~7g of seawood meal are standby
Material；Each component is mixed, adjusts PH to 7.1~7.5 after stirring and dissolving, in 120 DEG C of high-temperature sterilizations 15 minutes, waits culture medium temperature
Degree is sub-packed in after being cooled to less than 60 DEG C in sterilizing flask；Root circle protection bacterium is inoculated in the culture medium.
A kind of 7. preparation side of the soil improvement according to claim 6 with plant health with active complex micro organism fungicide
Method, it is characterised in that the egg 8g, milk 6g, dregs of beans 7g, rice bran 6g, seawood meal 5g.
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| CN201711302722.2A CN107988102A (en) | 2017-12-11 | 2017-12-11 | A kind of soil improvement and plant health activity complex micro organism fungicide |
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| CN201711302722.2A CN107988102A (en) | 2017-12-11 | 2017-12-11 | A kind of soil improvement and plant health activity complex micro organism fungicide |
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| CN101088966A (en) * | 2006-06-13 | 2007-12-19 | 北京新纪元三色生态科技有限公司 | Microbial fertilizer and its prepn process and application |
| US20080236227A1 (en) * | 2007-04-02 | 2008-10-02 | Flynn Timothy M | Dry land erosion control using photosynthetic nitrogen-fixing microorganisms |
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| CN106478210A (en) * | 2015-08-28 | 2017-03-08 | 山东横店草业畜牧有限公司 | A kind of multifunctional composite microbe fertilizer material and preparation method thereof |
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2017
- 2017-12-11 CN CN201711302722.2A patent/CN107988102A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101088966A (en) * | 2006-06-13 | 2007-12-19 | 北京新纪元三色生态科技有限公司 | Microbial fertilizer and its prepn process and application |
| US20080236227A1 (en) * | 2007-04-02 | 2008-10-02 | Flynn Timothy M | Dry land erosion control using photosynthetic nitrogen-fixing microorganisms |
| CN106478210A (en) * | 2015-08-28 | 2017-03-08 | 山东横店草业畜牧有限公司 | A kind of multifunctional composite microbe fertilizer material and preparation method thereof |
| CN105595010A (en) * | 2015-09-01 | 2016-05-25 | 嘉兴佳康丽生物科技有限公司 | Water quality balance ferment for aquaculture and organic sewage treatment and preparation method thereof |
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| 何国庆等: "《食品微生物学 第3版》", 30 September 2016, 中国农业大学出版社 * |
| 何若天等: "《农用益生菌生产与应用手册》", 29 February 2016, 金盾出版社 * |
| 蔡信之: "《合理施肥实用技术》", 31 July 1996, 上海科学技术文献出版社 * |
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Application publication date: 20180504 |