CN107986919A - A kind of microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation - Google Patents
A kind of microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation Download PDFInfo
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- CN107986919A CN107986919A CN201810071293.0A CN201810071293A CN107986919A CN 107986919 A CN107986919 A CN 107986919A CN 201810071293 A CN201810071293 A CN 201810071293A CN 107986919 A CN107986919 A CN 107986919A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
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Abstract
The invention discloses a kind of microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation, it is made of the component of following parts by weight:30~70 parts of stalk fermentation lactic acid waste material, 5~10 parts of stalk fermentation waste material charcoal, 1~10 part of complex micro organism fungicide, 5~20 parts of plant ash, 3~10 parts of potassium carbonate, 3~10 parts of ammonium phosphate, 3~15 parts of diatomite, 3~10 parts of soluble starch, 3~10 parts of urea.The microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation of the present invention, it can balancedly, constantly supply the nutritional ingredients such as nitrogen, phosphorus, potassium and the various trace elements needed for plant growth, nutrient is comprehensive, realize fertilizer efficiency combination of long drives and drop shots, nutrition supply speed combines, soil fertility can be effectively lifted, improves soil texture, eliminates soil hardening.The present invention also causes the waste material after stalk fermentation lactic acid to prepare microbial-bacterial fertilizer commercialization, reaches better effects in large-scale production processing.
Description
Technical field
The present invention relates to a kind of microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation and preparation method thereof, belong to microorganism fertilizer
Expect technical field.
Background technology
Biological organic fertilizer refers to a kind of microorganism containing work and can obtain specific fertilizer effect in use, and then is lifted
Crop yield or the biological agent for improving quality.Vital movement mistake of the microorganism in its fermentation and soil in biological organic fertilizer
The invalid nutrition in soil that can degrade in journey is effective nutrition, is directly absorbed for crop, produces fertilizer effect, while can produce
Raw substantial amounts of physiological activator, so as to produce effect of increasing production.Microorganism in bio-fertilizer is in plant root raised growth, numerous
Grow, form beneficial bacterium ecology, suppress and reduce the breeding chance of pathogen, what is played the role of also has antagonism pathogen, rises
The effect of having arrived mitigation crop disease, so as to reduce the use of pesticide, reduces Productive statistics.At present, China's biological organic fertilizer is sent out
It is fine to open up the impetus, forms bio-organic fertilizer industry, and the important member being increasingly becoming in fertilizer family.
China is large agricultural country, and stalk resource enriches, but its effective rate of utilization is very low at present, and scientific and technological content is few.Main
Land use systems have:Stalk is as inferior fuel, its stalk directly enters soil corruption for fertilizer, straw-returning after crop harvesting;Also
Considerable part agricultural crop straw discards field.Agricultural crop straw is scientifically developed, it is pair that Pfansteihl fermentation is carried out to stalk
One kind of stalk efficiently uses, but its waste material produced is not yet fully used, and needs further to be researched and developed.
After carrying out Pfansteihl fermentation to stalk, in waste material containing be not decomposed largely, the cellulose of utilization and conversion, partly
The materials such as cellulose, trace element, enzyme, lactic acid;The waste material of stalk fermentation lactic acid is reused, biological organic is made
Fertilizer, the protection of this development to national economy, clean manufacturing and ecological environment, reduces Productive statistics, is all feasible and necessary
's.
The content of the invention
For the above-mentioned prior art, the present invention provides a kind of microbial-bacterial fertilizer and its system of the waste material of lactic acid containing stalk fermentation
Preparation Method, this method step is simple, and production efficiency is high, is easy to be implemented in production practices.
The present invention is achieved by the following technical solutions:
A kind of microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation, is made of the component of following parts by weight:Stalk fermentation lactic acid gives up
30~70 parts of material, 5~10 parts of stalk fermentation waste material charcoal, 1~10 part of complex micro organism fungicide, 5~20 parts of plant ash, potassium carbonate 3
~10 parts, 3~10 parts of ammonium phosphate, 3~15 parts of diatomite, 3~10 parts of soluble starch, 3~10 parts of urea;
The stalk fermentation lactic acid waste material is prepared by the following method to obtain:Waste material after stalk fermentation lactic acid, adds carbon
Sour calcium adjusts pH to 6.0~7.0, and moisture≤5% is dried at 40~50 DEG C(Percetage by weight), crush, it is spare;
The stalk fermentation waste material charcoal is prepared by the following method to obtain:Waste material after stalk fermentation lactic acid is dried to water
It is divided into 15%~20%(Percetage by weight), put into charing kettle, under nitrogen atmosphere(Nitrogen is passed through to replace air), progressively rise
Temperature is kept for ± 3 minutes 90 minutes to 450 DEG C ± 10 DEG C, then heats to 900 DEG C ± 10 DEG C, is passed through vapor and is reacted 30 minutes
± 3 minutes;Naturally room temperature is down to, up to stalk fermentation waste material charcoal, is taken out, it is spare;
The complex micro organism fungicide, is pulvis or granule, containing saccharomycete, phosphate solubilizing bacteria, potassium solubilizing bacteria, bacillus subtilis,
Nitrogen-fixing bacteria and photosynthetic bacteria, its living bacteria count are:Saccharomycete 1 × 1010Cfu/g, phosphate solubilizing bacteria 1 × 1010Cfu/g, potassium solubilizing bacteria 1
×1010Cfu/g, bacillus subtilis 1 × 1010Cfu/g, nitrogen-fixing bacteria 1 × 1010Cfu/g, photosynthetic bacteria 1 × 1010cfu/g。
The preparation method of the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation is:Each component is added into stirred tank, is stirred
Mix uniformly, obtain microbial-bacterial fertilizer semi-finished product;Pelletize in dry granulating machine, sieve, dry, up to microbial-bacterial fertilizer finished product;
Testing index:Viable count >=108cfu/g。
Waste material after the stalk fermentation lactic acid, is stalk(Such as maize straw)Waste material, stalk are obtained after fermentation lactic acid
The method of fermentation lactic acid can be as follows:
(1)The pretreatment of stalk:Stalk is crushed, cross 60 mesh sieves, mixed with mixed liquor, the two mass ratio be 0.5~1:2.5
~3.5, stir evenly, 110 DEG C~130 DEG C processing 90 min~130 min;After treatment, washing, separation of solid and liquid, obtains
Filtrate and filter residue, it is spare;
The mixed liquor is by ionic liquid and water by weight 1~2:1 mixes, and the ionic liquid is selected from quaternary amines chemical combination
Thing, season phosphorus species, glyoxaline compound, azoles, halogen-based compounds, tetrafluoroborate class compound, six
Any one in fluorine phosphorus species or two or more combinations, preferably -3 methyl-imidazoles diethyl phosphate salt of 1- ethyls, N-
Butyl-pyridinium nitrate ([BPy] NO3);
(2)The enzymolysis of filter residue:Enzyme preparation is mixed with above-mentioned filter residue, the vigor of enzyme preparation is 60,000~100,000 U/g, 30 DEG C~
4h~10h is digested at 40 DEG C;Washed 2 times after enzymolysis, filter, obtain enzymolysis liquid and filter residue, carbonic acid is added into enzymolysis liquid
Calcium, the addition of calcium carbonate is 20%~40%(Calculated by the weight of enzymolysis liquid);The filter residue is useless after stalk fermentation lactic acid
Material;
The enzyme preparation is prepared by the following method to obtain:Cellulase is dissolved in hac buffer, to obtain the final product;
(3)Inoculation, fermentation:Rhizopus oryzae after activation is accessed into step(2)In obtained enzymolysis liquid, inoculum concentration 10%~15%
(Percentage by volume);Ferment the h of 12 h~24, after fermentation, obtains Pfansteihl.
The present invention the waste material of lactic acid containing stalk fermentation microbial-bacterial fertilizer, can balancedly, constantly supply plant growth
The nutritional ingredients such as required nitrogen, phosphorus, potassium and various trace elements, nutrient is comprehensive, realizes fertilizer efficiency combination of long drives and drop shots, and nutrition supply is fast
It is slow to combine, soil fertility can be effectively lifted, improves soil texture, eliminates soil hardening.The present invention is also so that stalk fermentation lactic acid
Waste material afterwards prepares microbial-bacterial fertilizer commercialization, reaches better effects in large-scale production processing.
Embodiment
With reference to embodiment, the present invention is further illustrated.
Involved instrument, reagent, material etc. in following embodiments, are existing in the prior art unless otherwise noted
Conventional instrument, reagent, material etc., can be obtained by regular commercial sources.Involved experimental method in following embodiments, inspection
Survey method etc., is existing normal experiment method, detection method etc. in the prior art unless otherwise noted.
Embodiment 1 prepares microbial-bacterial fertilizer using stalk fermentation lactic acid waste material
Microbial-bacterial fertilizer is made of the component of following parts by weight:63 parts of stalk fermentation lactic acid waste material, 5 parts of stalk fermentation waste material charcoal,
5 parts of complex micro organism fungicide, 5 parts of plant ash, 3 parts of potassium carbonate, 3 parts of ammonium phosphate, 10 parts of diatomite, 3 parts of soluble starch, urea
3 parts;
The stalk fermentation lactic acid waste material is prepared by the following method to obtain:Waste material after stalk fermentation lactic acid, adds carbon
Sour calcium adjusts pH to 6.5, and moisture≤5% is dried at 40~50 DEG C(Percetage by weight), crush, it is spare;
The stalk fermentation waste material charcoal is prepared by the following method to obtain:Waste material after stalk fermentation lactic acid is dried to water
It is divided into 18%(Percetage by weight), put into charing kettle, under nitrogen atmosphere(Nitrogen is passed through to replace air), progressively it is warming up to
450 DEG C, kept for 90 minutes, then heat to 900 DEG C, be passed through vapor and react 30 minutes;Naturally room temperature is down to, is sent out up to stalk
Ferment waste material charcoal, takes out, spare;
The complex micro organism fungicide, is pulvis or granule, containing saccharomycete, phosphate solubilizing bacteria, potassium solubilizing bacteria, bacillus subtilis,
Nitrogen-fixing bacteria and photosynthetic bacteria, its living bacteria count are:Saccharomycete 1 × 1010Cfu/g, phosphate solubilizing bacteria 1 × 1010Cfu/g, potassium solubilizing bacteria 1
×1010Cfu/g, bacillus subtilis 1 × 1010Cfu/g, nitrogen-fixing bacteria 1 × 1010Cfu/g, photosynthetic bacteria 1 × 1010cfu/g。
Preparation method is:Each component is added into stirred tank, is stirred evenly, obtains microbial-bacterial fertilizer semi-finished product;In dry method system
Pelletize in grain machine, sieve, dry, up to microbial-bacterial fertilizer finished product;Testing index:Viable count >=108cfu/g。
Waste material after the stalk fermentation lactic acid, obtains by the following method:
(1)The pretreatment of stalk:By corn straw smashing, cross 60 mesh sieves, mixed with mixed liquor, the two mass ratio be 1:2.5
Stir evenly, 120 DEG C of 120 min of processing;After treatment, washing, separation of solid and liquid, obtains filtrate and filter residue, spare;
The mixed liquor is by ionic liquid and water by weight 1:1 mixes, -3 methyl-imidazoles of ionic liquid 1- ethyls
Diethyl phosphate salt;
(2)The enzymolysis of filter residue:Enzyme preparation is mixed with above-mentioned filter residue, the vigor of enzyme preparation is 80,000 U/g, and 6h is digested at 35 DEG C;
Washed 2 times after enzymolysis, filter, obtain enzymolysis liquid and filter residue, calcium carbonate is added into enzymolysis liquid, the addition of calcium carbonate is
30%(Calculated by the weight of enzymolysis liquid);The filter residue is the waste material after stalk fermentation lactic acid;
The enzyme preparation is prepared by the following method to obtain:Cellulase is dissolved in hac buffer, to obtain the final product.
Embodiment 2 prepares microbial-bacterial fertilizer using stalk fermentation lactic acid waste material
Microbial-bacterial fertilizer is made of the component of following parts by weight:30 parts of stalk fermentation lactic acid waste material, 10 parts of stalk fermentation waste material charcoal,
10 parts of complex micro organism fungicide, 10 parts of plant ash, 5 parts of potassium carbonate, 5 parts of ammonium phosphate, 10 parts of diatomite, 5 parts of soluble starch, urine
5 parts of element.
The other the same as in Example 1.
Embodiment 3 prepares microbial-bacterial fertilizer using stalk fermentation lactic acid waste material
Microbial-bacterial fertilizer is made of the component of following parts by weight:38 parts of stalk fermentation lactic acid waste material, 10 parts of stalk fermentation waste material charcoal,
10 parts of complex micro organism fungicide, 20 parts of plant ash, 3 parts of potassium carbonate, 3 parts of ammonium phosphate, 10 parts of diatomite, 3 parts of soluble starch, urine
3 parts of element.
The other the same as in Example 1.
Experimental microbial Effect of Bacteria Fertilizer is tested
The microbial-bacterial fertilizer produced using above-described embodiment 1,2,3 is experimental group 1,2,3, the common biology of commercially available Hebei treasured field fertilizer industry
Bacterial manure is control group, and to the yield of early rice, processing maturation period and disease resistance etc. are made comparisons.Place is in Harbin Fangzheng County, plantation
Rice varieties are paddy rich 16, and plantation month is May, and harvest month is October, rice experimental field A1, A2, A3And A4(It is corresponding in turn to
Experimental group 1,2,3 and control group)Adjacent and soil physico-chemical property is similar, the content of organic matter 2.34%, salt content 1.9 ‰, pH6.5,
The common 10000m of cultivated area2.Experimental group 1,2,3 applies microbial-bacterial fertilizer prepared by embodiment 1,2,3 respectively, is base using opportunity
Fertilizer, amount of application are 3~4 pounds/acre, and use is uniformly mixed with fine earth.Control group applies commercially available common micro-organisms bacterial manure, applies
Opportunity, amount of application, method of application are same as above.Irrigation, field management etc. are identical with general planting.
After harvest, statistics per mu yield, growth cycle, leaf spot incidence, soil salt content, content of organic matter etc., contrast
It the results are shown in Table 1(Data are average value in table).
The influence of 1 microbial-bacterial fertilizer of the present invention of table and common bacterial manure to rice
By table 1 as it can be seen that the microbial-bacterial fertilizer of the present invention, obvious effect of increasing production, and can effectively suppress leaf spot, can also shorten rice
Production cycle, hence it is evident that better than commercially available common micro-organisms bacterial manure, especially experimental group 1, compared to the control group, increase production up to 30%, growth is all
Phase shortens nearly two weeks, and the incidence of leaf spot is also significantly lower than control group.
Claims (10)
- A kind of 1. microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation, it is characterised in that:It is made of the component of following parts by weight:Straw 30~70 parts of stalk fermentation lactic acid waste material, 5~10 parts of stalk fermentation waste material charcoal, 1~10 part of complex micro organism fungicide, plant ash 5~ 20 parts, 3~10 parts of potassium carbonate, 3~10 parts of ammonium phosphate, 3~15 parts of diatomite, 3~10 parts of soluble starch, 3~10 parts of urea.
- 2. the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to claim 1, it is characterised in that:The stalk hair Kefir milk acid waste products is prepared by the following method to obtain:Waste material after stalk fermentation lactic acid, adds calcium carbonate adjustment pH to 6.0 ~7.0, moisture≤5% is dried at 40~50 DEG C, is crushed, it is spare.
- 3. the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to claim 1, it is characterised in that:The stalk hair Ferment waste material charcoal is prepared by the following method to obtain:Waste material after stalk fermentation lactic acid is dried to that moisture is 15%~20%, In input charing kettle, under nitrogen atmosphere, 450 DEG C ± 10 DEG C are progressively warming up to, are kept for ± 3 minutes 90 minutes, then heated to 900 DEG C ± 10 DEG C, it is passed through vapor and reacts ± 3 minutes 30 minutes;Naturally room temperature is down to, it is spare up to stalk fermentation waste material charcoal.
- 4. the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to claim 1, it is characterised in that:It is described compound micro- Bacteria agent, is pulvis or granule, contains saccharomycete, phosphate solubilizing bacteria, potassium solubilizing bacteria, bacillus subtilis, nitrogen-fixing bacteria and photosynthetic thin Bacterium, its living bacteria count are:Saccharomycete 1 × 1010Cfu/g, phosphate solubilizing bacteria 1 × 1010Cfu/g, potassium solubilizing bacteria 1 × 1010Cfu/g, withered grass Bacillus 1 × 1010Cfu/g, nitrogen-fixing bacteria 1 × 1010Cfu/g, photosynthetic bacteria 1 × 1010cfu/g。
- 5. the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to Claims 2 or 3, it is characterised in that:The straw Waste material after stalk fermentation lactic acid obtains by the following method:(1)The pretreatment of stalk:Stalk is crushed, cross 60 mesh sieves, mixed with mixed liquor, the two mass ratio be 0.5~1:2.5 ~3.5, stir evenly, 110 DEG C~130 DEG C processing 90 min~130 min;After treatment, washing, separation of solid and liquid, obtains Filtrate and filter residue, it is spare;The mixed liquor is by ionic liquid and water by weight 1~2:1 mixes, and the ionic liquid is selected from quaternary amines chemical combination Thing, season phosphorus species, glyoxaline compound, azoles, halogen-based compounds, tetrafluoroborate class compound, six Any one in fluorine phosphorus species or two or more combinations;(2)The enzymolysis of filter residue:Enzyme preparation is mixed with above-mentioned filter residue, the vigor of enzyme preparation is 60,000~100,000 U/g, 30 DEG C~ 4h~10h is digested at 40 DEG C;Washed 2 times after enzymolysis, filter, obtain enzymolysis liquid and filter residue, carbonic acid is added into enzymolysis liquid Calcium, the addition of calcium carbonate is 20%~40%;The filter residue is the waste material after stalk fermentation lactic acid;The enzyme preparation is prepared by the following method to obtain:Cellulase is dissolved in hac buffer, to obtain the final product.
- 6. the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to claim 5, it is characterised in that:The ionic liquid Body is selected from -3 methyl-imidazoles diethyl phosphate salt of 1- ethyls or N- butyl-pyridinium nitrate.
- 7. according to the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to any one of claims 1 to 6, its feature exists In:It is made of the component of following parts by weight:63 parts of stalk fermentation lactic acid waste material, 5 parts of stalk fermentation waste material charcoal, composite microbial bacteria 5 parts of agent, 5 parts of plant ash, 3 parts of potassium carbonate, 3 parts of ammonium phosphate, 10 parts of diatomite, 3 parts of soluble starch, 3 parts of urea.
- 8. according to the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to any one of claims 1 to 6, its feature exists In:It is made of the component of following parts by weight:30 parts of stalk fermentation lactic acid waste material, 10 parts of stalk fermentation waste material charcoal, complex microorganism 10 parts of microbial inoculum, 10 parts of plant ash, 5 parts of potassium carbonate, 5 parts of ammonium phosphate, 10 parts of diatomite, 5 parts of soluble starch, 5 parts of urea.
- 9. according to the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to any one of claims 1 to 6, its feature exists In:It is made of the component of following parts by weight:38 parts of stalk fermentation lactic acid waste material, 10 parts of stalk fermentation waste material charcoal, complex microorganism 10 parts of microbial inoculum, 20 parts of plant ash, 3 parts of potassium carbonate, 3 parts of ammonium phosphate, 10 parts of diatomite, 3 parts of soluble starch, 3 parts of urea.
- 10. the preparation method of the microbial-bacterial fertilizer of the waste material of lactic acid containing stalk fermentation according to any one of claims 1 to 9, its It is characterized in that:Each component is added into stirred tank, is stirred evenly, obtains microbial-bacterial fertilizer semi-finished product;Pelletize in dry granulating machine, Screening, drying, up to microbial-bacterial fertilizer.
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Cited By (1)
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CN108863616A (en) * | 2018-09-14 | 2018-11-23 | 安徽养地生态科技有限公司 | A kind of method that the fermentation of stalk two-phase prepares fertilizer |
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KR101505563B1 (en) * | 2014-07-15 | 2015-03-26 | 양지개발 주식회사 | Seed spray soil composition for eco-friendly wet-type greening works having excellent coherence of soil and greening works using the same |
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