CN107969339B - Anoectochilus roxburghii cultivation method - Google Patents
Anoectochilus roxburghii cultivation method Download PDFInfo
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Abstract
The invention provides a method for cultivating anoectochilus formosanus, which comprises the steps of transplanting the whole field anoectochilus formosanus plant with soil into an artificial greenhouse, carrying out artificial pollination on the flag of the anoectochilus formosanus plant, and picking when the fruit is mature; obtaining seed embryos of picked fruits, sowing the seed embryos in a germination culture medium for culturing to obtain protocorms; transferring the protocorm into a differentiation culture medium, and culturing for 23-24 days at the temperature of 26-27 ℃ and the illumination intensity of 1500-; then transferring the bud seedlings into a strengthening culture medium, and culturing for 30 days at the temperature of 26-27 ℃ and the illumination intensity of 2000-3000Lux in the illumination time of 12 hours per day to obtain strong seedlings; and then, the gravity is gradually enhanced to stimulate in the rooting culture process, so that the developed degree of the root system of the anoectochilus formosanus seedlings can be obviously enhanced, and the survival rate and the rapid growth probability of transplanting are further improved.
Description
Technical Field
The invention relates to the field of plant cultivation methods, in particular to a method for cultivating anoectochilus formosanus.
Background
The cultivation method of anoectochilus formosanus is known, and as the Chinese patent of invention, CN201410383427.4 discloses a seedling cultivation method of anoectochilus formosanus, comprising the following steps: firstly, selecting robust anoectochilus formosanus with clear leaf veins and a flowering period, taking a section of stem of 2-4 cm between an overground part and an underground part of the anoectochilus formosanus, mashing the rest part to form an anoectochilus formosanus solution, adding 1-2% of tween-20 into the anoectochilus formosanus solution, then placing the stem into the anoectochilus formosanus solution, and treating for 24-72 hours at 4 ℃; step two, placing the main stem treated in the step one on 1/3-1/2 MS solid culture medium to grow for 10-15 days to obtain rooted seedlings, wherein full-day illumination is adopted during the culture period, the illumination intensity is 1000-1500Lux, and the culture temperature is 24-28 ℃; step three, transferring the seedlings obtained in the step two to 1/4-1/3 MS liquid culture medium for culturing for 50-60 days, adding the anoectochilus formosanus solution in the step one to the 1/4-1/3 MS liquid culture medium, and carrying out long-day photoperiod irradiation during culturing, wherein the light period is 16 hours, the illumination intensity is 2000-2500 Lux, the temperature is 28 ℃, the dark period is 8 hours, and the temperature is 22 ℃; step four, filling a solid matrix into a cup-shaped container made of degradable materials, transplanting the seedlings obtained in the step three into the solid matrix for culturing for 20-40 days, culturing under the same conditions as those in the step three in the 1 st-3 th day, and culturing for 4 th-40 th days under the illumination of 16h in the illumination period, wherein the illumination intensity is 3000-3500 Lux, the temperature is 30 ℃, the dark period is 8h, and the temperature is 22 ℃, wherein the solid matrix comprises the following components in parts by weight: 10-20 parts of soil, 15-30 parts of vermiculite, 3-6 parts of humus, 10-20 parts of bananas, 1-2 parts of mangos, 2-4 parts of mangosteen peels and 1-2 parts of dragon fruit peels; the air humidity is 80% -95% in the culture process, and the cup-shaped container is made of any one of polylactic acid, polyvinyl alcohol and polyester materials; MS culture medium formula:
macroelements:
1650mg/L NH4NO3，1900mg/L KNO3，440mg/L CaCl2·2H2O，370mg/L MgSO4·7H2o and 700mg/L KH2PO4；
Trace elements:
0.83mg/L KI，6.25mg/L H3BO3，22.3mg/LMnSO4·4H2O，8.65mg/L ZnSO4·7H2O，0.25mg/LNa2MnO4·2H2O，0.025mg/L CuSO4·5H2o and 0.025mg/LCoCl2·6H2O
Iron salt:
27.8mg/L FeSO4·7H2o and 7.3mg/L Na2-EDTA·2H2O3
30mg/L of sucrose, 8mg/L of agar powder and Tris and MES are added to adjust the pH to 5.80, thus obtaining an MS solid culture medium; the MS liquid culture medium is obtained without adding 8mg/L agar powder; 1/3MS solid culture medium containing macroelements, microelements, iron salt and sucrose 1/3 of MS culture medium, adding agar powder 8mg/L, and adjusting pH to 5.80 with Tris and MES to obtain 1/3MS solid culture medium; 1/2MS solid culture medium containing macroelements, microelements, iron salt and sucrose 1/2 of MS culture medium, adding agar powder 8mg/L, and adjusting pH to 5.80 with Tris and MES to obtain 1/2MS solid culture medium; 1/4MS liquid culture medium containing macroelements, microelements, iron salt and sucrose 1/4 of MS culture medium, and adjusting pH to 5.80 with Tris and MES to obtain 1/4MS liquid culture medium; 1/3MS liquid culture medium containing macroelements, microelements, iron salt and sucrose 1/3 of MS culture medium, and adjusting pH to 5.80 with Tris and MES to obtain 1/3MS liquid culture medium. And (4) crushing the main stem which does not root in the step two and the seedling with the height of less than 2cm in the step three, and adding the crushed main stem and the crushed seedling into the solid matrix. The solid matrix comprises the following components in parts by weight: 15 parts of soil, 18 parts of vermiculite, 4 parts of humus, 15 parts of bananas, 1 part of mangoes, 3 parts of mangosteen peels and 1 part of dragon fruit peels. And (3) crushing the bananas, mangoes, mangosteen peels and dragon fruit peels into particles with the particle size of less than 40 meshes. In the third step, 1/4-1/3 MS liquid culture medium is prepared by using plasma water.
The anoectochilus roxburghii seedlings cultured by the method can be directly transplanted into soil without transplantation, but similar to other culturing methods in the market, the seedlings cultured by the method have the defects of underdeveloped root systems, low transplanting survival rate and low growth speed.
Accordingly, the present inventors have made extensive studies to solve the above problems and have made the present invention.
Disclosure of Invention
The invention aims to provide a method for cultivating anoectochilus formosanus, which can obviously enhance the developed degree of the root system of the anoectochilus formosanus seedlings, further improve the survival rate and the rapid growth probability of transplanting and has strong practicability.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for cultivating anoectochilus formosanus comprises the following steps:
(1) transplanting the whole field anoectochilus roxburghii plant with soil into an artificial greenhouse, carrying out artificial pollination on the anoectochilus roxburghii plant flags, and picking after the fruits are ripe;
(2) obtaining embryo of picked fruit, sowing the embryo in germination culture medium, and culturing at 26-27 deg.C and illumination intensity of 1000-;
(3) transferring the protocorm into a differentiation culture medium, and culturing for 23-24 days at the temperature of 26-27 ℃ and the illumination intensity of 1500-;
(4) transferring the bud seedlings into a strengthening culture medium, and culturing for 30 days at the temperature of 26-27 ℃ and the illumination intensity of 2000-;
(5) transferring the strong seedlings into a rooting device for rooting culture;
the rooting device comprises a rotating frame, a lighting device arranged in the rotating frame and a fixed protective cover covering the rotating frame; the rotating frame comprises a transparent rotating cylinder which is vertically arranged, a plurality of layers of culture chambers which are arranged on the peripheral surface of the transparent rotating cylinder in an up-and-down manner, a support which is borne below the transparent rotating cylinder, and a rotation driving device which is arranged on the support and is used for driving the transparent rotating cylinder to rotate; the lighting device is arranged in the transparent rotating cylinder, and the lighting device is vertical and is coaxial with the transparent rotating cylinder;
the culture chamber comprises a plurality of culture cavities which are uniformly and annularly arranged around the axis of the transparent rotating cylinder and a plurality of culture units which are positioned in the culture cavities one by one and are connected with the peripheral surface of the transparent rotating cylinder; the culture chambers are separated by horizontal partition plates, the culture cavities are separated by vertical partition plates, and the horizontal partition plates and the vertical partition plates are connected with the peripheral surface of the transparent rotating cylinder; the culture unit comprises a radial telescopic rod and a culture hanging basket, wherein the radial telescopic rod extends along the radial direction of the transparent rotating cylinder and is connected with the outer peripheral surface of the transparent rotating cylinder, and the culture hanging basket is pivoted at the outer end of the radial telescopic rod; the upper end of the culture hanging basket and the outer end of the radial telescopic rod are pivoted together through a horizontal rotating shaft vertical to the radial telescopic rod; the culture hanging basket comprises an upper containing part for containing a solid rooting culture medium, a lower containing part for containing a culture solution and positioned below the upper containing part, and a deformation chamber positioned below the lower containing part and used for allowing the lower containing part to deform; the bottom of the upper containing part is an elastic water-permeable net layer, the bottom of the lower containing part is a water-impermeable elastic layer, and the bottom of the deformation chamber is a breathable supporting net for the deformation chamber to breathe;
in the rooting culture process, a solid rooting culture medium is filled in the upper containing part of each culture hanging basket, then a culture solution is poured into the solid rooting culture medium, and after being absorbed by the solid rooting culture medium, the redundant culture solution is stored in the lower containing part through the elastic water-permeable net layer; planting a plurality of strong seedlings into the solid culture medium of each upper containing part one by one and fixing;
then starting the rotation driving device to drive the transparent rotating cylinder to rotate gradually, wherein each cultivation hanging basket is turned outwards by virtue of a horizontal rotating shaft, and when each cultivation hanging basket rotates around the axis of the transparent rotating cylinder at a constant speed, each cultivation hanging basket and the axis of the transparent rotating cylinder form a stable inclination angle; because of the existence of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is greater than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is greater than the gravity of each culture solution, each elastic breathable net and each elastic layer generate elastic deformation arching downwards, part of the culture solution absorbed in each solid rooting culture medium can permeate into the lower containing part, but most of the culture solution still remains in each solid rooting culture medium to perform sufficient water culture on the strong seedlings; simultaneously starting the lighting device to light the strong seedlings in each culture hanging basket, keeping the strong seedlings at the temperature of 26-27 ℃ and carrying out first-stage rotary culture for 10 days in the lighting time of 12 hours each day, wherein the lighting intensity is 2000-3000 Lux;
then adjusting the rotation driving device to increase the rotation speed of each culture hanging basket by 50 percent, keeping the illumination intensity at 2000-3000Lux, keeping the strong seedlings at the temperature of 26-27 ℃, and carrying out second-stage rotation culture for 10 days in 12-hour illumination time every day; due to the increase of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is more than the gravity of each culture solution, each elastic breathable net and each elastic layer generate downward arched elastic deformation, most of the culture solution absorbed in each solid rooting culture medium permeates into the lower containing part, and only a small part of the culture solution is reserved in each solid rooting culture medium to perform water-deficient culture on strong seedlings;
then adjusting the rotation driving device to improve the rotation speed of each culture hanging basket by 50 percent, keeping the illumination intensity at 2000-3000Lux, keeping the strong seedlings at the temperature of 26-27 ℃, and carrying out the third-stage rotation culture of 15 days of culture in 12 hours per day of illumination time; due to the further increase of the centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is more than the gravity of each culture solution, each elastic breathable net and each elastic layer generate elastic deformation arching downwards, but each elastic layer is supported by each corresponding breathable supporting net, so that each solid rooting culture medium is soaked in the culture solution of each lower containing part to perform sufficient water culture on strong seedlings;
obtaining a rooted seedling after three-stage rotary culture;
(6) taking the rooted seedlings out one by one for transplanting.
In the step (5), adjusting each radial telescopic rod to enable the rotation radius of each culture basket to be 0.5-2 m; in the first section of rotary culture, the rotary driving device is adjusted to ensure that the linear speed of rotation of each culture basket is 1-5 m/s; in the second stage of rotary culture, the rotary driving device is adjusted to ensure that the linear speed of rotation of each culture hanging basket is 1.5-7.5 m/s; in the third stage of rotary culture, the rotary driving device is adjusted to ensure that the linear speed of rotation of each culture basket is 2.25-11.25 m/s.
In the step (5), an air inlet chamber is arranged at the upper part of the fixed protective cover, and a first air inlet is formed at the upper end of the air inlet chamber; a transition air chamber is formed in the transparent rotating cylinder, a plurality of second air inlets communicated with the air inlet chambers are formed at the upper end of the transition air chamber, a plurality of third air inlets corresponding to the upper parts of the culture chambers are formed in the transparent rotating cylinder, a plurality of chamber doors corresponding to the culture chambers one by one are arranged on the fixed protective cover, and air outlets communicated with the lower parts of the corresponding culture chambers are arranged on the chamber doors; the second air inlets are uniformly and annularly arranged around the axis of the transparent rotating cylinder, and the air inlet ends of the second air inlets are provided with first air inlet nozzles facing the rotating direction of the transparent rotating cylinder; the air inlet end of the third air inlet is provided with a second air inlet nozzle facing the rotation direction of the transparent rotating cylinder; the air outlet is provided with an adjusting switch capable of adjusting the air flow of the air outlet;
in the rooting culture process, along with the rotation of the transparent rotating cylinder, air in the air inlet chamber enters the second air inlets through the first air inlet nozzles and enters the transition air chamber, air in the transition air chamber enters the third air inlets through the second air inlet nozzles and provides air for strong seedlings, and the air in the culture chambers flows out through the air outlets to form continuous flowing air in the culture chambers; the air outlet quantity of each air outlet can be adjusted by operating each adjusting switch, and further, the air flow in each culture cavity is adjusted.
After the technical scheme is adopted, the method for cultivating the anoectochilus formosanus comprises the steps of transplanting the whole field anoectochilus formosanus plants into an artificial greenhouse with soil, carrying out artificial pollination on the flags of the anoectochilus formosanus plants, and picking fruits after the fruits are ripe to obtain the fruits; then, seed embryos of picked fruits are obtained, the seed embryos are sown in a germination culture medium, and are cultured for 23-24 days under the conditions of temperature of 26-27 ℃ and illumination intensity of 1000-; transferring the protocorm into a differentiation culture medium, and culturing for 23-24 days at the temperature of 26-27 ℃ and the illumination intensity of 1500-; then transferring the bud seedlings into a strengthening culture medium, and culturing for 30 days at the temperature of 26-27 ℃ and the illumination intensity of 2000-3000Lux in the illumination time of 12 hours per day to obtain strong seedlings; then in the process of rooting culture, a solid rooting culture medium is filled in the upper containing part of each culture hanging basket, then culture solution is poured into the solid rooting culture medium, and after the culture solution is absorbed by the solid rooting culture medium, the redundant culture solution is stored in the lower containing part through the elastic water-permeable net layer; planting a plurality of strong seedlings into the solid culture medium of each upper containing part one by one and fixing; then starting a rotation driving device to drive the transparent rotating cylinder to rotate gradually, enabling each cultivation hanging basket to turn outwards by virtue of a respective horizontal rotating shaft, and enabling each cultivation hanging basket and the axis of the transparent rotating cylinder to form a stable inclination angle when each cultivation hanging basket rotates around the axis of the transparent rotating cylinder at a constant speed; because of the existence of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is greater than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is greater than the gravity of each culture solution, each elastic breathable net and each elastic layer generate elastic deformation arching downwards, part of the culture solution absorbed in each solid rooting culture medium can permeate into the lower containing part, but most of the culture solution still remains in each solid rooting culture medium to perform sufficient water culture on the strong seedlings; meanwhile, the lighting device is started to light the strong seedlings in each culture hanging basket, the illumination intensity is 2000-; because strong seedlings are subjected to the combined action of centripetal acceleration and gravity acceleration, the gravity action on the strong seedlings is increased equivalently, the stimulation effect of the strong seedlings of the anoectochilus formosanus on the gravity can be enhanced by utilizing the dependence of the growth of the roots of the anoectochilus formosanus plants on the gravity, the fast rooting of the strong seedlings is facilitated, in addition, sufficient water sources in the stage can provide favorable conditions for the initial rooting, and the first stage of rotating culture can also be used as an adaptation stage of the strong seedlings to the gravity enhancement; after the first stage of rotary culture is finished, the root system of the strong seedling is 10-30% stronger than that of the strong seedling cultured by the common method in the same period. Then adjusting a rotation driving device to improve the rotation speed of each culture hanging basket by 50 percent, keeping the illumination intensity at 2000-3000Lux, keeping the strong seedlings at the temperature of 26-27 ℃, and carrying out second-stage rotation culture for 10 days in 12-hour illumination time every day; because of the increase of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is more than the gravity of each culture solution, each elastic breathable net and each elastic layer generate elastic deformation arching downwards, most of the culture solution absorbed in each solid rooting culture medium permeates into the lower containing part, and only a small part of the culture solution is reserved in each solid rooting culture medium to perform water-deficient culture on strong seedlings; after the adaptation stage of strong seedlings to the gravity enhancement is finished, the second stage of rotating culture further enhances the stimulation effect of the strong anoectochilus formosanus seedlings by the gravity, so that the strong seedlings can further take root, and the insufficient water source in the stage can further stimulate the roots of the strong seedlings to extend downwards and outwards to obtain more water sources by means of physiologically and naturally searching for the main roots and the diffusion roots, so that the strong seedlings can be rapidly increased; after the second stage of rotary culture is finished, the root system of the strong seedling can be increased by 30-50% compared with the strong seedling cultured by the common method in the same period. Then adjusting a rotation driving device to improve the rotation speed of each culture hanging basket by 50 percent, keeping the illumination intensity at 2000-3000Lux, keeping the strong seedlings at the temperature of 26-27 ℃, and carrying out a third-stage rotation culture of 15 days in 12-hour illumination time every day; because of the further increase of the centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is more than the gravity of each culture solution, each elastic breathable net and each elastic layer generate elastic deformation arching downwards, but each elastic layer is supported by each corresponding breathable supporting net at the moment, so that each solid rooting culture medium is soaked in the culture solution of each lower containing part to perform sufficient water culture on the strong seedlings; after the water-deficient culturing stage of the second stage of rotary culture is finished, the second stage of rotary culture is sufficient for supplementing water on the basis of further enhancing the stimulation effect of the strong anoectochilus formosanus seedlings by gravity, so that the thirsty of the strong seedlings due to long-term water deficiency is relieved, sufficient water sources are provided for the growth of the root systems of the strong seedlings, and further the further thickening and root system strengthening of the strong seedlings are facilitated; after the third stage of rotary culture is finished, the root system of the strong seedling can be thickened by 60-90% compared with the strong seedling cultured by the common method in the same period. After three-stage rotary culture, the rooting seedlings with thick and strong root systems are obtained, and a favorable foundation is provided for survival and rapid growth of subsequent transplantation; then taking the rooted seedlings out one by one for transplanting. Compared with the prior art, the method for cultivating the anoectochilus formosanus can obviously enhance the developed degree of the root system of the anoectochilus formosanus seedlings, further improve the survival rate and the rapid growth probability of transplanting, and has strong practicability.
Drawings
FIG. 1 is a schematic view of a partial cross-sectional structure of a rooting device in a resting state;
FIG. 2 is a schematic view of a root device in partial cross-section in a rotational state;
FIG. 3 is a top view, partially in section, of the rooting device;
FIG. 4 is a schematic sectional view of the culture basket.
In the figure:
11-transparent rotary cylinder 111-transition air chamber 1111-second air inlet b 1-first air inlet nozzle 112-third air inlet nozzle 1121-second air inlet nozzle
12-culture chamber 120-horizontal partition 121-culture cavity 1210-vertical partition
122-culture unit 1221-radial telescopic rod 1222-culture basket a 1-upper containing part a 11-elastic water permeable net layer a 2-lower containing part a 21-elastic layer a 3-deformation chamber a 31-air permeable support net
13-support 14-rotation driving device
2-lighting device 3-fixed protective cover 31-air inlet chamber 311-first air inlet 32-chamber door 321-air outlet 3211-regulating switch.
Detailed Description
In order to further explain the technical solution of the present invention, the following detailed description is given by way of specific examples.
The invention discloses a method for cultivating anoectochilus formosanus, which comprises the following steps as shown in figures 1-4:
(1) transplanting the whole field anoectochilus roxburghii plant with soil into an artificial greenhouse, carrying out artificial pollination on the anoectochilus roxburghii plant flags, and picking after the fruits are ripe;
(2) obtaining embryo of picked fruit, sowing the embryo in germination culture medium, and culturing at 26-27 deg.C and illumination intensity of 1000-; the germination culture medium can be MS, AC1-3g/L, sucrose 32-35g/L and agar 7-8g/L, and pH is 5.8;
(3) transferring the protocorm into a differentiation culture medium, and culturing for 23-24 days at the illumination time of 12 hours per day under the conditions of the temperature of 26-27 ℃, the illumination intensity of 1500-; the differentiation medium is MS, potato 220-250g/L, AC1-3g/L, sucrose 32-35g/L and agar 7-8g/L, pH5.8;
(4) transferring the bud seedling into a strengthening culture medium, and culturing for 30 days at the illumination time of 12 hours per day under the conditions of the temperature of 26-27 ℃ and the illumination intensity of 2000-3000(2000, 2200, 2400, 2600, 2800 or 3000) Lux to obtain a strengthened seedling; the strengthening culture medium comprises MS, banana juice 120-L, AC1-3g/L, sucrose 32-35g/L and agar 7-8g/L, and the pH value is 5.8;
(5) transferring the strong seedlings into a rooting device for rooting culture;
the rooting device comprises a rotating frame, a lighting device 2 arranged in the rotating frame and a fixed protective cover 3 covered outside the rotating frame; the rotating frame comprises a transparent rotating cylinder 11 which is vertically arranged, a plurality of layers of culture chambers 12 which are arranged on the peripheral surface of the transparent rotating cylinder 11 in an up-and-down manner, a support 13 which is born below the transparent rotating cylinder 11, and a rotation driving device 14 which is arranged on the support 13 and drives the transparent rotating cylinder 11 to rotate; the lighting device 2 is arranged in the transparent rotating cylinder 11, and the lighting device 2 is vertical and is coaxial with the transparent rotating cylinder 11; the rotation driving device 14 can be a motor and is connected with the bottom of the transparent rotating cylinder 11 through a transmission shaft, a circular sliding rail coaxial with the transparent rotating cylinder 11 is formed on the upper surface of the support 13, a circular sliding groove matched with the circular sliding rail is formed at the bottom of the transparent rotating cylinder 11, and the circular sliding rail is matched with the circular sliding groove to stably guide and support the transparent rotating cylinder 11;
the culture chamber 12 includes a plurality of culture chambers 121 arranged in a uniform ring around the axis of the transparent rotary cylinder 11, and a plurality of culture units 122 located one by one in each culture chamber 121 and connected to the outer circumferential surface of the transparent rotary cylinder 11; the culture chambers 12 are separated by horizontal partition plates 120, the culture cavities 121 are separated by vertical partition plates 1210, and the horizontal partition plates 120 and the vertical partition plates 1210 are connected with the outer peripheral surface of the transparent rotary cylinder 11; the cultivation unit 122 includes a radial expansion link 1221 extending in a radial direction of the transparent rotary cylinder 11 and connected to an outer circumferential surface of the transparent rotary cylinder 11, and a cultivation basket 1222 pivoted to an outer end of the radial expansion link 1221; the upper end of the cultivation hanging basket 1222 and the outer end of the radial telescopic rod 1221 are pivoted together through a horizontal rotating shaft perpendicular to the radial telescopic rod 1221; the cultivation hanging basket 1222 comprises an upper containing part a1 for containing solid rooting culture medium, a lower containing part a2 for containing culture solution and located below the upper containing part a1, and a deformation chamber a3 located below the lower containing part a2 and used for allowing the lower containing part a2 to deform; the bottom of the upper containing part a1 is an elastic water-permeable net layer a11, the bottom of the lower containing part a2 is a water-impermeable elastic layer a21, and the bottom of the deformation chamber a3 is an air-permeable supporting net a31 for the deformation chamber a3 to breathe;
in the rooting culture process, a solid rooting culture medium is filled in the upper containing part a1 of each culture hanging basket 1222, then the culture solution is poured into the solid rooting culture medium, and after the culture solution is absorbed by the solid rooting culture medium, the redundant culture solution is stored in the lower containing part a2 through the elastic water-permeable net layer a 11; planting a plurality of strong seedlings into the solid culture medium of each upper containing part a1 one by one and fixing; culturing each strong seedling in an independent culture chamber 121;
then, the rotation driving device 14 is started to drive the transparent rotating cylinder 11 to rotate gradually, each culture basket 1222 is turned outwards by virtue of the respective horizontal rotating shaft, and each culture basket 1222 forms a stable inclination angle with the axis of the transparent rotating cylinder 11 when each culture basket 1222 rotates around the axis of the transparent rotating cylinder 11 at a constant speed; because of the existence of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is greater than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer a21 is greater than the gravity of each culture solution, each elastic breathable net and each elastic layer a21 generate elastic deformation arching downwards, part of the culture solution absorbed in each solid rooting culture medium can permeate into the lower containing part a2, but most of the culture solution still remains in each solid rooting culture medium to carry out sufficient water culture on the strong seedlings; simultaneously, the lighting device 2 is started to light the strong seedlings in each culture hanging basket 1222, the illumination intensity is 2000-3000(2000, 2200, 2400, 2600, 2800 or 3000) Lux, the strong seedlings are kept at the temperature of 26-27 ℃, and the first period of rotating culture is carried out for 10 days with the illumination time of 12 hours each day;
then adjusting the rotation driving device 14 to increase the rotation speed of each culture basket by 50%, the illumination intensity is 2000-3000(2000, 2200, 2400, 2600, 2800 or 3000) Lux, and the strong seedlings are maintained at the temperature of 26-27 ℃, and the second stage of rotation culture of culturing is carried out for 10 days with the illumination time of 12 hours each day; due to the increase of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer a21 is more than the gravity of each culture solution, each elastic breathable net and each elastic layer a21 generate elastic deformation which arches downwards, most of the culture solution absorbed in each solid rooting culture medium permeates into the lower containing part a2, and only a small part of the culture solution is reserved in each solid rooting culture medium to perform water-deficient culture on strong seedlings;
then adjusting the rotation driving device 14 to increase the rotation speed of each culture basket by 50%, the illumination intensity is 2000-3000(2000, 2200, 2400, 2600, 2800 or 3000) Lux, and the strong seedlings are maintained at the temperature of 26-27 ℃, and the third section of rotation culture of 15 days is carried out with the illumination time of 12 hours per day; because the centripetal acceleration is further increased, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer a21 is more than the gravity of each culture solution, each elastic breathable net and each elastic layer a21 generate elastic deformation which is arched downwards, but each elastic layer a21 is supported by each corresponding breathable supporting net a31, so that each solid rooting culture medium is soaked in the culture solution of each lower containing part a2 to perform sufficient water culture on the strong seedlings; the rooting culture medium is 1/2MS, NAA0.8-1.2mg/L, sucrose 25-30g/L, and semisolid or solid substances such as agar and active carbon in balance, and has pH of 5.8;
obtaining a rooted seedling after three-stage rotary culture;
(6) taking the rooted seedlings out one by one for transplanting. Specifically, the culture medium attached to the root of the rooting seedling is cleaned, humus or pond sludge is used as a transplanting matrix, the temperature of the transplanted rooting seedling is kept at 24-26 ℃, the humidity is 78-80%, and the survival rate reaches more than 96%.
In the actual cultivation process, the whole field anoectochilus roxburghii plant is transplanted into an artificial greenhouse with soil, artificial pollination is carried out on the anoectochilus roxburghii plant flags, and picking is carried out when fruits are ripe to obtain the fruits; then, seed embryos of picked fruits are obtained, the seed embryos are sown in a germination culture medium, and are cultured for 23-24 days under the conditions of temperature of 26-27 ℃ and illumination intensity of 1000-; transferring the protocorm into a differentiation culture medium, and culturing for 23-24 days at the temperature of 26-27 ℃ and the illumination intensity of 1500-; then transferring the bud seedlings into a strengthening culture medium, and culturing for 30 days at the temperature of 26-27 ℃ and the illumination intensity of 2000-3000Lux in the illumination time of 12 hours per day to obtain strong seedlings; then in the process of rooting culture, solid rooting culture medium is filled in the upper containing part a1 of each culture hanging basket 1222, then culture solution is poured into the solid rooting culture medium, and after the culture solution is absorbed by the solid rooting culture medium, the redundant culture solution is stored in the lower containing part a2 through the elastic water-permeable net layer a 11; planting a plurality of strong seedlings into the solid culture medium of each upper containing part a1 one by one and fixing; then, the rotation driving device 14 is started to drive the transparent rotating cylinder 11 to rotate gradually, each culture basket 1222 is turned outwards by virtue of the respective horizontal rotating shaft, and each culture basket 1222 forms a stable inclination angle with the axis of the transparent rotating cylinder 11 when each culture basket 1222 rotates around the axis of the transparent rotating cylinder 11 at a constant speed; because of the existence of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is greater than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer a21 is greater than the gravity of each culture solution, each elastic breathable net and each elastic layer a21 generate elastic deformation arching downwards, part of the culture solution absorbed in each solid rooting culture medium can permeate into the lower containing part a2, but most of the culture solution still remains in each solid rooting culture medium to carry out sufficient water culture on the strong seedlings; meanwhile, the lighting device 2 is started to light the strong seedlings in each culture hanging basket 1222 with the illumination intensity of 2000-; because strong seedlings are subjected to the combined action of centripetal acceleration and gravity acceleration, the gravity action on the strong seedlings is increased equivalently, the stimulation effect of the strong seedlings of the anoectochilus formosanus on the gravity can be enhanced by utilizing the dependence of the growth of the roots of the anoectochilus formosanus plants on the gravity, the fast rooting of the strong seedlings is facilitated, in addition, sufficient water sources in the stage can provide favorable conditions for the initial rooting, and the first stage of rotating culture can also be used as an adaptation stage of the strong seedlings to the gravity enhancement; after the first stage of rotary culture is finished, the root system of the strong seedling is 10-30% stronger than that of the strong seedling cultured by the common method in the same period. Then adjusting the rotation driving device 14 to increase the rotation speed of each culture basket by 50%, the illumination intensity is 2000-; due to the increase of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer a21 is more than the gravity of each culture solution, each elastic breathable net and each elastic layer a21 generate elastic deformation which arches downwards, most of the culture solution absorbed in each solid rooting culture medium permeates into the lower containing part a2, and only a small part of the culture solution is reserved in each solid rooting culture medium to perform water-deficient culture on strong seedlings; after the adaptation stage of strong seedlings to the gravity enhancement is finished, the second stage of rotating culture further enhances the stimulation effect of the strong anoectochilus formosanus seedlings by the gravity, so that the strong seedlings can further take root, and the insufficient water source in the stage can further stimulate the roots of the strong seedlings to extend downwards and outwards to obtain more water sources by means of physiologically and naturally searching for the main roots and the diffusion roots, so that the strong seedlings can be rapidly increased; after the second stage of rotary culture is finished, the root system of the strong seedling can be increased by 30-50% compared with the strong seedling cultured by the common method in the same period. Then adjusting the rotation driving device 14 to increase the rotation speed of each culture basket by 50%, the illumination intensity is 2000-3000Lux, and strong seedlings are maintained at the temperature of 26-27 ℃, and the third section of rotation culture of 15 days is carried out in the illumination time of 12 hours every day; because the centripetal acceleration is further increased, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer a21 is more than the gravity of each culture solution, each elastic breathable net and each elastic layer a21 generate elastic deformation which is arched downwards, but each elastic layer a21 is supported by each corresponding breathable supporting net a31, so that each solid rooting culture medium is soaked in the culture solution of each lower containing part a2 to perform sufficient water culture on the strong seedlings; after the water-deficient culturing stage of the second stage of rotary culture is finished, the second stage of rotary culture is sufficient for supplementing water on the basis of further enhancing the stimulation effect of the strong anoectochilus formosanus seedlings by gravity, so that the thirsty of the strong seedlings due to long-term water deficiency is relieved, sufficient water sources are provided for the growth of the root systems of the strong seedlings, and further the further thickening and root system strengthening of the strong seedlings are facilitated; after the third stage of rotary culture is finished, the root system of the strong seedling can be thickened by 60-90% compared with the strong seedling cultured by the common method in the same period. After three-stage rotary culture, the rooting seedlings with thick and strong root systems are obtained, and a favorable foundation is provided for survival and rapid growth of subsequent transplantation; then taking the rooted seedlings out one by one for transplanting. The specific structure can be that still be equipped with temperature detection sensor and heater block in the transparent rotary drum 11, can adjust the temperature in the fixed protection casing 3 for the gold thread lotus seedling.
Preferably, in step (5), each radial expansion link 1221 is adjusted so that the radius of rotation of each cultivation basket 1222 is 0.5-2 m; each radial expansion link 1221 specifically comprises an outer tube connected with the transparent rotating cylinder 11, and an inner rod slidably sleeved in the outer tube and connected with the upper end of the cultivation basket 1222; the side wall of the outer pipe is provided with a propping bolt, and the outer pipe is provided with a screw hole for the propping bolt to pass through and prop against to be positioned on the inner rod; an operator can adjust the extending length of the inner rod by loosening the abutting bolt, further adjust the rotating radius of the culture hanging basket 1222 and then tighten the abutting bolt to position the inner rod; when the radius of rotation of the cultivation basket 1222 is less than 0.5m, the maximum efficiency of the rotation driving device 14 in the form of a motor is not easy to release, and the number of the cultivation cavities 121 of each layer of cultivation room 12 is too small, the number of the strong seedlings can be accommodated is too small, which is not beneficial to cultivation and has high cost; when the rotating radius of the cultivation hanging basket 1222 is larger than 2m, the motor load is too large, the rotating speed of the cultivation hanging basket 1222 is not easy to be increased, and the rotation of the cultivation hanging basket 1222 is unstable, so that the cultivation hanging basket 1222 is easy to shake up and down, and is not beneficial to rooting of strong seedlings; when the rotating radius of the culture hanging basket 1222 is 0.5-2m, all parameters of the whole device are adjusted, which is beneficial to strong seedling and rooting, especially when the rotating radius of the culture hanging basket 1222 is 1m, the test cost is the lowest and the efficiency is high;
in the first stage of rotary culture, the rotary driving device 14 is adjusted to make the linear speed of rotation of each culture basket 1222 to be 1-5 m/s; in the first stage of rotary cultivation, when the rotation linear speed of the cultivation hanging basket 1222 is lower than 1m/s, the enhanced sensitivity of strong seedlings to gravity is not high, and the root systems of the strong seedlings are not obviously robust compared with the root systems of strong seedlings cultivated by a common method at the same time; when the rotation linear speed of the culture hanging basket 1222 is higher than 5m/s, the strong seedling has too high sensitivity to gravity, the strong seedling can not adapt to the sudden increase of gravity, and the rooting rate of the strong seedling is lower than 20% and even dies; when the rotating linear speed of the cultivation hanging basket 1222 is 1-5m/s, the strong seedlings can adapt to the enhancement of gravity, the root systems of the strong seedlings can be 10-30% stronger than those cultivated by a common method at the same time, and especially when the rotating linear speed of the cultivation hanging basket 1222 is 3m/s, the root systems of the strong seedlings can be 30% stronger than those cultivated by a common method at the same time, and the highest value is achieved; after the first stage of rotary culture, the root system of the strong seedling can reach 1-3cm long.
In the second stage of rotary culture, the rotary driving device 14 is adjusted to make the linear speed of rotation of each culture basket 1222 to be 1.5-7.5 m/s; in the second stage of rotary culture, because the strong seedlings adapt to the gravity of the first stage of rotary culture, when the rotating linear speed of the culture hanging basket 1222 is lower than 1.5m/s, the enhanced sensitivity of the strong seedlings to the gravity is not high, and the root systems of the strong seedlings are not obviously robust compared with the root systems of the strong seedlings cultured by a common method at the same time; when the rotation linear speed of the culture hanging basket 1222 is higher than 7.5m/s, the sensitivity of strong seedlings to gravity is too high, strong seedlings cannot adapt to the sudden increase of gravity, and the growth rate of the root systems of the strong seedlings is lower than 20% and even die; when the rotating linear speed of the cultivation hanging basket 1222 is 1.5-7.5m/s, the strong seedlings can adapt to the enhancement of gravity, the root systems of the strong seedlings can be 30-50% stronger than those cultivated by a common method at the same period, and especially when the rotating linear speed of the cultivation hanging basket 1222 is 4.5m/s, the root systems of the strong seedlings can be 50% stronger than those cultivated by a common method at the same period, and the highest value is achieved; after the second stage of rotating culture, the root system of the strong seedling can reach 4-7cm long.
In the third stage of rotary culture, the rotary driving device 14 is adjusted to make the linear speed of rotation of each culture basket 1222 to be 2.25-11.25 m/s; in the third stage of rotary culture, because the strong seedlings adapt to the gravity of the second stage of rotary culture, when the rotation linear speed of the culture hanging basket 1222 is lower than 2.25m/s, the enhanced sensitivity of the strong seedlings to the gravity is not high, and the root systems of the strong seedlings are not obviously robust compared with the root systems of the strong seedlings cultured by a common method at the same time; when the rotation linear speed of the culture hanging basket 1222 is higher than 11.25m/s, the sensitivity of strong seedlings to gravity is too high, strong seedlings cannot adapt to the sudden increase of gravity, and the growth rate of the root systems of the strong seedlings is lower than 20% and even die; when the rotating linear speed of the cultivation hanging basket 1222 is 2.25-11.25m/s, the strong seedlings can adapt to the enhancement of gravity, the root systems of the strong seedlings can be 60-90% stronger than those cultivated by a common method in the same period, and especially when the rotating linear speed of the cultivation hanging basket 1222 is 6.75m/s, the root systems of the strong seedlings can be 90% stronger than those cultivated by a common method in the same period, and the highest value can be achieved. After the third section of rotary culture, the root system of the strong seedling can reach 7-10cm long.
When the strong seedling rooting is stimulated by gravity enhancement, the strong seedling is gradually enhanced to adapt to the normal growth or even die, the strong seedling cannot adapt to the normal growth or even die due to sudden enhancement, but the gravity enhancement cannot be too frequent, so that the normal growth of the root system of the strong seedling is influenced by vibration and the like. Wherein the water-deficient culture in the second stage of rotary culture is assisted gravity-enhanced stimulation, and the water-deficient culture can enhance the development degree of the root system by 10-20% in the second stage of rotary culture.
Preferably, in the step (5), the upper part of the fixed shield 3 is provided with an air inlet chamber 31, and the upper end of the air inlet chamber 31 is formed with a first air inlet 311; a transition air chamber 111 is formed inside the transparent rotating cylinder 11, a plurality of second air inlets 1111 communicated with the air inlet chamber 31 are formed at the upper end of the transition air chamber 111, a plurality of third air inlets 112 corresponding to the upper parts of the culture chambers 121 are formed on the transparent rotating cylinder 11, a plurality of chamber doors 32 corresponding to the culture chambers 121 one by one are arranged on the fixed protective cover 3, and air outlets 321 communicated with the lower parts of the corresponding culture chambers 121 are arranged on the chamber doors 32; the second air inlets 1111 are uniformly and annularly arranged around the axis of the transparent rotating cylinder 11, and the air inlet ends of the second air inlets 1111 are provided with first air inlet nozzles b1 facing the rotating direction of the transparent rotating cylinder 11; the air inlet end of the third air inlet 112 is provided with a second air inlet nozzle 1121 facing the rotation direction of the transparent rotating cylinder 11; the air outlet 321 is provided with an adjusting switch 3211 capable of adjusting the air flow of the air outlet 321;
in the process of rooting culture, along with the rotation of the transparent rotating cylinder 11, air in the air inlet chamber 31 enters the second air inlets 1111 through the first air inlet nozzles b1 and enters the transition air chamber 111, air in the transition air chamber 111 enters the third air inlets 112 through the second air inlet nozzles 1121 and provides air for the strong seedlings in the culture cavities 121, and air in the culture cavities 121 flows out through the air outlets 321 to form continuous flowing air in the culture cavities 121; the air output from each air outlet 321 can be adjusted by operating each adjustment switch 3211, thereby adjusting the air flow in each culture chamber 121. The operator can operate each anoectochilus roxburghii seedling through each chamber door 32. And operating personnel can let in the air that contains more carbon dioxide by first air inlet 311 daytime, let in the air that contains more oxygen by first air inlet 311 night, do benefit to gold thread lotus seedling growth.
The product form of the present invention is not limited to the embodiments and examples shown in the present application, and any suitable changes or modifications of the similar ideas should be made without departing from the patent scope of the present invention.
Claims (2)
1. A method for cultivating anoectochilus formosanus is characterized by comprising the following steps:
(1) transplanting the whole field anoectochilus roxburghii plant with soil into an artificial greenhouse, carrying out artificial pollination on the anoectochilus roxburghii plant flags, and picking after the fruits are ripe;
(2) obtaining embryo of picked fruit, sowing the embryo in germination culture medium, and culturing at 26-27 deg.C and illumination intensity of 1000-;
(3) transferring the protocorm into a differentiation culture medium, and culturing for 23-24 days at the temperature of 26-27 ℃ and the illumination intensity of 1500-;
(4) transferring the bud seedlings into a strengthening culture medium, and culturing for 30 days at the temperature of 26-27 ℃ and the illumination intensity of 2000-;
(5) transferring the strong seedlings into a rooting device for rooting culture;
the rooting device comprises a rotating frame, a lighting device arranged in the rotating frame and a fixed protective cover covering the rotating frame; the rotating frame comprises a transparent rotating cylinder which is vertically arranged, a plurality of layers of culture chambers which are arranged on the peripheral surface of the transparent rotating cylinder in an up-and-down manner, a support which is borne below the transparent rotating cylinder, and a rotation driving device which is arranged on the support and is used for driving the transparent rotating cylinder to rotate; the lighting device is arranged in the transparent rotating cylinder, and the lighting device is vertical and is coaxial with the transparent rotating cylinder;
the culture chamber comprises a plurality of culture cavities which are uniformly and annularly arranged around the axis of the transparent rotating cylinder and a plurality of culture units which are positioned in the culture cavities one by one and are connected with the peripheral surface of the transparent rotating cylinder; the culture chambers are separated by horizontal partition plates, the culture cavities are separated by vertical partition plates, and the horizontal partition plates and the vertical partition plates are connected with the peripheral surface of the transparent rotating cylinder; the culture unit comprises a radial telescopic rod and a culture hanging basket, wherein the radial telescopic rod extends along the radial direction of the transparent rotating cylinder and is connected with the outer peripheral surface of the transparent rotating cylinder, and the culture hanging basket is pivoted at the outer end of the radial telescopic rod; the upper end of the culture hanging basket and the outer end of the radial telescopic rod are pivoted together through a horizontal rotating shaft vertical to the radial telescopic rod; the culture hanging basket comprises an upper containing part for containing a solid rooting culture medium, a lower containing part for containing a culture solution and positioned below the upper containing part, and a deformation chamber positioned below the lower containing part and used for allowing the lower containing part to deform; the bottom of the upper containing part is an elastic water-permeable net layer, the bottom of the lower containing part is a water-impermeable elastic layer, and the bottom of the deformation chamber is a breathable supporting net for the deformation chamber to breathe;
in the rooting culture process, a solid rooting culture medium is filled in the upper containing part of each culture hanging basket, then a culture solution is poured into the solid rooting culture medium, and after being absorbed by the solid rooting culture medium, the redundant culture solution is stored in the lower containing part through the elastic water-permeable net layer; planting a plurality of strong seedlings into the solid culture medium of each upper containing part one by one and fixing;
then starting the rotation driving device to drive the transparent rotating cylinder to rotate gradually, wherein each cultivation hanging basket is turned outwards by virtue of a horizontal rotating shaft, and when each cultivation hanging basket rotates around the axis of the transparent rotating cylinder at a constant speed, each cultivation hanging basket and the axis of the transparent rotating cylinder form a stable inclination angle; because of the existence of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is greater than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is greater than the gravity of each culture solution, each elastic breathable net and each elastic layer generate elastic deformation arching downwards, part of the culture solution absorbed in each solid rooting culture medium can permeate into the lower containing part, but most of the culture solution still remains in each solid rooting culture medium to perform sufficient water culture on the strong seedlings; simultaneously starting the lighting device to light the strong seedlings in each culture hanging basket, keeping the strong seedlings at the temperature of 26-27 ℃ and carrying out first-stage rotary culture for 10 days in the lighting time of 12 hours each day, wherein the lighting intensity is 2000-3000 Lux;
then adjusting the rotation driving device to increase the rotation speed of each culture hanging basket by 50 percent, keeping the illumination intensity at 2000-3000Lux, keeping the strong seedlings at the temperature of 26-27 ℃, and carrying out second-stage rotation culture for 10 days in 12-hour illumination time every day; due to the increase of centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is more than the gravity of each culture solution, each elastic breathable net and each elastic layer generate downward arched elastic deformation, most of the culture solution absorbed in each solid rooting culture medium permeates into the lower containing part, and only a small part of the culture solution is reserved in each solid rooting culture medium to perform water-deficient culture on strong seedlings;
then adjusting the rotation driving device to improve the rotation speed of each culture hanging basket by 50 percent, keeping the illumination intensity at 2000-3000Lux, keeping the strong seedlings at the temperature of 26-27 ℃, and carrying out the third-stage rotation culture of 15 days of culture in 12 hours per day of illumination time; due to the further increase of the centripetal acceleration, the pressure of each solid rooting culture medium on each elastic breathable net is more than the gravity of each solid rooting culture medium, the pressure of each culture solution on each elastic layer is more than the gravity of each culture solution, each elastic breathable net and each elastic layer generate elastic deformation arching downwards, but each elastic layer is supported by each corresponding breathable supporting net, so that each solid rooting culture medium is soaked in the culture solution of each lower containing part to perform sufficient water culture on strong seedlings;
obtaining a rooted seedling after three-stage rotary culture;
(6) taking the rooted seedlings out one by one for transplanting;
in the step (5), adjusting each radial telescopic rod to enable the rotation radius of each culture basket to be 0.5-2 m; in the first section of rotary culture, the rotary driving device is adjusted to ensure that the linear speed of rotation of each culture basket is 1-5 m/s; in the second stage of rotary culture, the rotary driving device is adjusted to ensure that the linear speed of rotation of each culture hanging basket is 1.5-7.5 m/s; in the third stage of rotary culture, the rotary driving device is adjusted to ensure that the linear speed of rotation of each culture basket is 2.25-11.25 m/s.
2. The method for cultivating anoectochilus formosanus according to claim 1, wherein the method comprises the following steps: in the step (5), an air inlet chamber is arranged at the upper part of the fixed protective cover, and a first air inlet is formed at the upper end of the air inlet chamber; a transition air chamber is formed in the transparent rotating cylinder, a plurality of second air inlets communicated with the air inlet chambers are formed at the upper end of the transition air chamber, a plurality of third air inlets corresponding to the upper parts of the culture chambers are formed in the transparent rotating cylinder, a plurality of chamber doors corresponding to the culture chambers one by one are arranged on the fixed protective cover, and air outlets communicated with the lower parts of the corresponding culture chambers are arranged on the chamber doors; the second air inlets are uniformly and annularly arranged around the axis of the transparent rotating cylinder, and the air inlet ends of the second air inlets are provided with first air inlet nozzles facing the rotating direction of the transparent rotating cylinder; the air inlet end of the third air inlet is provided with a second air inlet nozzle facing the rotation direction of the transparent rotating cylinder; the air outlet is provided with an adjusting switch capable of adjusting the air flow of the air outlet;
in the rooting culture process, along with the rotation of the transparent rotating cylinder, air in the air inlet chamber enters the second air inlets through the first air inlet nozzles and enters the transition air chamber, air in the transition air chamber enters the third air inlets through the second air inlet nozzles and provides air for strong seedlings, and the air in the culture chambers flows out through the air outlets to form continuous flowing air in the culture chambers; the air outlet quantity of each air outlet can be adjusted by operating each adjusting switch, and further, the air flow in each culture cavity is adjusted.
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| CN109042323A (en) * | 2018-08-13 | 2018-12-21 | 儒兰（福建）生物科技有限公司 | A kind of roxburgh anoectochilus terminal bud culture medium and roxburgh anoectochilus terminal bud breeding method |
| CN109566324B (en) * | 2018-11-23 | 2021-02-09 | 泉州市金草生物技术有限公司 | Seed selection and cultivation process of goldthread root |
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| CN104186314A (en) * | 2014-08-06 | 2014-12-10 | 宁明县红枫中药材种植专业合作社 | Anoectochilus formosanus seedling culturing method |
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| CN104186314A (en) * | 2014-08-06 | 2014-12-10 | 宁明县红枫中药材种植专业合作社 | Anoectochilus formosanus seedling culturing method |
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