CN202014494U - Plant tissue culture seedling exercising device - Google Patents

Plant tissue culture seedling exercising device Download PDF

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Publication number
CN202014494U
CN202014494U CN2010206809939U CN201020680993U CN202014494U CN 202014494 U CN202014494 U CN 202014494U CN 2010206809939 U CN2010206809939 U CN 2010206809939U CN 201020680993 U CN201020680993 U CN 201020680993U CN 202014494 U CN202014494 U CN 202014494U
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China
Prior art keywords
hardening
framework
window
supporting
seedling exercising
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CN2010206809939U
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Chinese (zh)
Inventor
李云
王树芝
孙宇涵
胡瑞阳
王新杰
田野
韩凤宝
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Beijing Forestry University
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Beijing Forestry University
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Abstract

The utility model discloses a moveable plant tissue culture seedling exercising device. The seedling exercising device comprises a seedling exercising framework, a plurality of seedling exercising rooms used for holding tissue culture seedlings and formed by dividing the seedling exercising framework by a plurality of partition boards, and a seedling exercising window mounted on the outer side of the seedling exercising framework. The moveable plant tissue culture seedling exercising device runs stably, has the functions of rain prevention, adjustment of light intensity, uniformity of light receiving of tube seedlings, capability of being moved at will, and the like, and is not influenced by seedling exercising fields. The seedling exercising device has the advantage that the light receiving faces of tube seedlings can be adjusted at any time, and gaps are not left between bottles when tube seedlings are placed, so that the utilization ratio of a seedling exercising space is increased, the seedling exercising time is shortened, and the seedling exercising efficiency is improved.

Description

A kind of hardening device of plant tissue culture seedlings-raising
Technical field
The present invention relates to a kind of plant tissue culture vessel arranging apparatus, particularly a kind of Plant Tissue Breeding seedling domestication culture apparatus belongs to the biological culture technigue field.
Background technology
Plant Tissue Breeding, be cultured in vitro again, finger is isolated tissue (as cambium, epidermis, cortex, marrow cell, endosperm etc.), organ (as root, stem, leaf, stem apex, flower, fruit etc.) or the cell (as megaspore, microspore, somatic cell etc.) that suits the requirements from plant corpus, protoplast etc., by sterile working, under the manual control condition, cultivate with whole plant that obtains regeneration or the technology of producing other products of economically valuable.The Plant Tissue Breeding of narrow sense is meant and utilizes plant each several part tissue, cultivate the acquisition regeneration plant as cambium, parenchyma, mesophyll tissue, endosperm etc., also refer to produce from each organ in incubation the cultivation of callus, callus is again through being differentiated to form aftergrowth again.
Plant tissue culture seedlings-raising may further comprise the steps: 1) preparation medium; 2) sterilization of medium; 3) aseptic inoculation; 4) cultivate, comprise initial culture, successive transfer culture, culture of rootage; 5) domestication is transplanted.Wherein, tissue cultivating seedling is owing to be to grow under near 100% environmental condition in aseptic, nutritious supply, suitable illumination, temperature and relative moisture, therefore, all with the seedling of natural conditions growth very big difference arranged at aspects such as physiology, forms.So must pass through hardening, for example by control water, lose weight, add lustre to, measure such as cooling, make tissue cultivating seedling little by little adapt to external environment, thereby corresponding the variation taken place at physiology, form, tissue, make it more appropriate to natural environment, only in this way could guarantee that test-tube plantlet transplants success smoothly.
From blade, the cuticula of tissue cultivating seedling is undeveloped, and blade does not have epidermal hair usually, or less epidermal hair is only arranged, even has occurred a large amount of water holes on the blade, and the quantity of pore, size also often surpass common seedling.Hence one can see that, and tissue cultivating seedling is more suitable for the ambient growth in high humidity, and when they were transplanted to the test tube external environment, the tissue cultivating seedling percentage of water loss can be very high, is very easy to dead.Therefore, for above-mentioned bad physiology, the form characteristics of improving tissue cultivating seedling, then must process handle with the domestication that the external world adapts, the measure of taking usually has: will increase humidity to external world, weaken illumination; To in vitro wanting penetrating gas, enrich carbon dioxide fertilizer, progressively reducing air humidity etc.
Current domesticating method to tissue cultivating seedling mainly be before transplanting can with plant tissue cultures cultivate vessel not the state of opening move down under the natural lighting and took exercise 2-15 days, allow tissue cultivating seedling accept the irradiation of sunlight, make its stalwartness of looking, and then will cultivate the vessel mouth and open, open state lower refining seedling 1-2 days, stand processing, to adapt to the condition of natural humidity in the future than low humidity.
At present also there is not proprietary device to be used for the hardening device of the tissue cultivating seedling of Plant Tissue Breeding, existing hardening all is that the culturing rack in the tissue culture procedures is transformed with equipment, remove the domestication of carrying out tissue cultivating seedling behind the fluorescent tube on the culturing rack, this hardening frame can not use separately, but must put into this hardening frame the hardening canopy or the greenhouse of shading facility simultaneously, just to be placed on the culturing rack that is used for hardening in the hardening canopy of light control bar spare or the greenhouse and just can carry out hardening, otherwise cause the test-tube plantlet sun burn, damage the seedling of taking root, be difficult to reach the hardening effect, and adopt the culturing rack of transforming to carry out hardening and handle, test-tube plantlet has only a sensitive surface, this sensitive surface receives the natural daylight abundance, and other faces reception natural daylights are few, cause the test-tube plantlet hardening inhomogeneous, a side of promptly facing the test-tube plantlet of sunlight is subjected to light many, hardening is effective, and opposite side is because of being subjected to light few, the hardening weak effect.In order to make test-tube plantlet be subjected to light even, then need in the hardening process, adopt artificial one bottle of one bottle of ground to put test-tube plantlet again at set intervals, adjust the sensitive surface of test-tube plantlet, it is time-consuming therefore to take merit, and efficiency is low, has increased the seedling cost of tissue cultivating seedling.
Culturing rack with test-tube plantlet carries out the hardening processing as the hardening device to test-tube plantlet at present, this culturing rack mostly is ironwork or wooden goods, be cuboid, height is 2 meters-2.5 meters, long is 1.25-1.3 rice, wide is 0.4-0.6 rice, establish the 5-7 layer more, whenever high layer by layer is 30-35 centimetre, use glass plate between layer and the layer, plank, wire netting is as dividing plate, put test-tube plantlet on the shelf and carry out hardening, except dividing plate, do not have other affiliated facilities, penetrating all around, can not carry out temperature adjustment effectively to test-tube plantlet, damping, windproof, sunshade and screening rain need in the hardening processing procedure hardening frame is placed on the hardening canopy, carry out in hardening greenhouse or the hardening plastic tunnel, can not be according to variety classes, the tissue cultivating seedling of different hardening degree carries out personalization adjustment, can only rely on next whole adjustment of facility such as hardening canopy, the hardening poor processing effect lacks specific aim, is difficult to satisfy the specific requirement of different plants.
Existing hardening frame can not carry out moving arbitrarily according to features such as the power of sunlight, directions, can not adjust the sensitive surface of test-tube plantlet, and uneven illumination is even in the hardening process, the hardening weak effect; And existing hardening device is unenclosed construction, the function of not shading, the exposure intensity that can not regulate sunlight according to the kind for the treatment of the tissue cultivating seedling that hardening is handled, characteristic etc.; In addition, existing hardening shelf for conveying difficulty is after the test-tube plantlet that needs to have taken root before test-tube plantlet carries out hardening is transported to the hardening place, putting on the hardening frame, needing after hardening finishes test-tube plantlet undercarriage and vanning carrying, the operating procedure that needs is many, inefficiency, inconvenient operation; Hardening place floor space is big, and existing hardening frame can not use separately, must have the hardening canopy or the greenhouse of shading to ensure, has high input the cost height.
Summary of the invention
The purpose of this utility model is the problem that exists at prior art, a kind of training tissue culture seedling device is provided, hardening device of the present invention can arbitrarily move, have simultaneously windproof, rainproof, can adjust intensity of illumination, tissue cultivating seedling according to the hardening needs and be subjected to functions such as light is even, and be not subjected to the influence of hardening place size, when test-tube plantlet is put in the hardening device bottle with bottle between do not interspace, improved the hardening utilization of space, shorten the hardening time, improved hardening efficient.
For realizing purpose of the present invention, one aspect of the present invention provides a kind of hardening device of plant tissue culture seedlings-raising, and this device comprises:
The hardening framework, by cuboid or square that four root posts are formed, its top, bottom and side are made up of the glass plate of light-permeable;
Dividing plate is arranged on the hardening framework from top to bottom, and the hardening framework is divided into the seeding room that several place tissue cultivating seedling;
The hardening window is installed in parallel in the front and back of described hardening framework, by opening or closing described hardening window, regulates the temperature and humidity in the described seeding room.
Wherein, also comprise shading screen, be fixed on hardening window top,, regulate the intensity of illumination in the seeding room by rolling or launch described shading screen.
Wherein, described shading screen (claiming sunshade net, shading net again) is a network structure, adopts ageing-resistant plastics silk braiding to form, and changes the shading screen with different shading rates according to different time or place.
Particularly, the hardening framework is iron or/and wood frame.
Wherein, with the position that the hardening framework is connected with dividing plate rotating shaft being set at the top of hardening framework, making the hardening window be fixed on the outside of hardening framework by rotating shaft, is that axle rotates with the rotating shaft, and being beneficial to the hardening window can open laterally.
Wherein, the hardening window is rectangle or square-shaped frame, and its inner glass plate that light-permeable is installed makes sunlight can see through glass plate and shines test-tube plantlet in the seeding room.
Particularly, the width of hardening window and the height of seeding room are to consistent, and the length of hardening window is consistent with the length of hardening framework.
Wherein, also comprise hardening window open system, be used to strut or close the hardening window.
Particularly, described hardening window open system comprises: the support footstock that is fixed on hardening reveal face; Support slide bar; Be fixed on the column of hardening frame facet, have 2 vertical mutually and intersect the base for supporting of through hole A, B, wherein, support footstock and be connected, and the support litter passes the through hole A on the base for supporting, in base for supporting, can move freely with the top of supporting slide bar.
Particularly, through hole B is cylindrical on the base for supporting, and its two ends diameter is inequality.
Especially, set gradually the slide bar fixed knob in the through hole B of base for supporting from top to bottom, support slide bar and bolt, wherein, fixed knob be pressed in support slide bar above, by tightening or unscrew fixed knob the support slide bar is free to slide in supporting seat, so that open or close the hardening window; Bolt is fixed on base for supporting on the column of hardening framework.
Particularly, also comprise anti-slide plate, be arranged on the both sides up and down of supporting slide bar, be positioned at the anti-slide plate that supports a side above the slide bar and contact with fixed knob, the anti-slide plate of opposite side contacts with bolt, when fixed knob is tightened, prevent to support slide bar and slide the fixed support slide bar.
Wherein, described hardening window open system comprises support ring, the support bar of the one-tenth semi-annular shape that is fixed on hardening reveal face and is fixed on support bar set bolt on the column of hardening frame facet that wherein, support bar is fixedlyed connected with set bolt.
Particularly, an end of described support bar is a hook-type, and the other end is an annular.
Wherein, an end of the support bar that is connected with support ring is a hook-type, and an end that is connected with set bolt is an annular.
Particularly, the annulus of support bar annular one end is fixedlyed connected with set bolt, and support bar is that the center is free to rotate with the set bolt.
Particularly, some semi-annular shape support rings are set, when opening the hardening window, support bar are connected with support ring, form different louver angles, regulate ventilation, temperature adjustment, damping situation in the seeding room at hardening reveal face.
Wherein, also comprise the shading screen that is installed in the hardening frame facet,, regulate the intensity of illumination in the seeding room by rolling or launch described shading screen.
Especially, the top layer at the hardening framework also is equipped with shading screen.
Wherein, also comprise the deflecting roller that is arranged on the hardening base of frame, the hardening device can be moved arbitrarily.
Particularly, described deflecting roller is installed in the bottom of four root posts of forming the hardening framework.
Hardening device of the present invention has following advantage:
1, this hardening apparatus function is complete, have windproof, rainproof, sunshade, arbitrarily function such as move, can adjust the intensity that tissue cultivating seedling receives illumination, make tissue cultivating seedling be subjected to light even, therefore can regulate according to factors such as hardening place, intensity of illumination, light irradiating angles, both saved hardening place and space, broken away from again simultaneously that the hardening device is taken shelter from rain to having, the hardening canopy of shading facility and the dependence in greenhouse.
2, this hardening device can use separately, does not need to be equipped with hardening canopy, seeding room, does not also need other conditions such as greenhouse, is not subjected to the restriction of objective condition in the conventional hardening process, has simplified the cultivation program of tissue cultivating seedling, has saved investment.
3, this hardening device can move arbitrarily or rotate, and can adjust the sensitive surface of tissue cultivating seedling at any time, makes the test-tube plantlet in the hardening process obtain uniform natural light irradiation by the sensitive surface of changing tissue cultivating seedling, has improved hardening effect and quality, has shortened the hardening time.
4, this hardening device is easy to use, and the hardening bottom of device has deflecting roller, can move arbitrarily, and is easy to operate, simplified the complicated loaded down with trivial details operation that needs to carry repeatedly test-tube plantlet in traditional hardening process, is beneficial to the large-scale production that tissue cultivating seedling is cultivated.
5, this hardening device utilization ratio height because of it adjusts test-tube plantlet sensitive surface function, can not interspace between bottle and bottle when putting test-tube plantlet, thereby improved the hardening utilization of space, increasing operation rate to reach 50%, has also shortened the hardening time simultaneously, and the hardening time shortens 30%.
6, the hardening window be opened or be closed to the hardening window of this hardening device can according to the height of the temperature and humidity in the seeding room in the training tissue culture seedling process, controls the temperature and humidity of training tissue culture seedling, improved the efficient of training tissue culture seedling.
7, the shading screen of this hardening device can be according to the exposure intensity of sunlight in the hardening process, the lighting effect of control sunlight, changed the defective that intensity of illumination can not artificially be controlled in the hardening process, according to the actual state of different hardening season, training tissue culture seedling, the number of plies of being replaced by the shading screen of different light transmittances or increase and decrease shading screen is controlled the lighting effect of natural daylight.
Description of drawings
Schematic perspective view when Fig. 1 is the unlatching of the utility model hardening device hardening window;
Fig. 2 is the side schematic view of the utility model hardening device hardening window;
Side schematic view when Fig. 3 closes for the utility model hardening device hardening window;
Side schematic view when Fig. 4 is the unlatching of the utility model hardening device hardening window;
Schematic top plan view when Fig. 5 closes for the utility model hardening device hardening window;
Schematic top plan view when Fig. 6 is the unlatching of the utility model hardening device hardening window;
The schematic diagram of open system when Fig. 7 is the unlatching of embodiment 1 hardening window;
The schematic diagram of open system when Fig. 8 closes for embodiment 1 hardening window;
Fig. 9 a is the enlarged diagram of analysing and observe of the base for supporting of embodiment 1 hardening window open system;
Fig. 9 b is base for supporting, support slide bar, the fixed knob front schematic view of embodiment 1 hardening window open system;
Fig. 9 c be embodiment 1 hardening window open system base for supporting, support slide bar, fixed knob perpendicular to the cross-sectional schematic in the column plane;
Figure 10 is the structural representation of hardening device shading screen box of the present invention;
The schematic diagram of the open system when Figure 11 opens for embodiment 2 hardening device hardening windows;
The schematic diagram of the open system when Figure 12 closes for embodiment 2 hardening device hardening windows;
Figure 13 a is support bar, the set bolt front schematic view of embodiment 2 hardening window open systems;
Figure 13 b is that the support bar, set bolt of embodiment 2 hardening window open systems is perpendicular to the cross-sectional schematic in the plane of column.
Description of reference numerals:
1, seeding room; 2, hardening window; 3, dividing plate; 4, hardening framework; 41, column; 5, deflecting roller; 6, rotating shaft; 7, hardening window opening device; 71, support footstock; 72, support slide bar; 73, base for supporting; 74A, 74B, through hole; 75, fixed knob; 76, anti-slide plate; 77, support ring; 78, support bar; 79, set bolt; 8, shading screen; 9, shading screen box; 91, shell; 92, axostylus axostyle; 93, rope-winding wheel; 94, bearing; 95, fixed head; 96, rope; 97, stage clip; 10, handle; 11, bolt.
Embodiment
Describe specific embodiment of the utility model with reference to the accompanying drawings in detail.
Embodiment 1
Referring to Fig. 1, hardening device of the present utility model is by hardening framework 4, dividing plate 3, seeding room 1, hardening window 2, hardening window open system 7, shading screen 15 and the mobile arbitrarily deflecting roller 5 of hardening device is formed.
The hardening framework of being made up of four root posts 41 4 is rectangular build or square build, its front, side, top and bottom are rectangle or/and square, it highly is 180-250cm, several layers dividing plate 3 is set on it from top to bottom, and described dividing plate 3 is divided into the seeding room 1 that several place tissue cultivating seedling with hardening framework 4.
Hardening framework 4 in the specific embodiment of the invention is iron framework, and integral body is rectangular build.The inner glass plate that light-permeable is installed of the framework of the side of hardening framework 4, top and bottom makes sunlight can see through glass plate and shines test-tube plantlet in the seeding room 1.
Wherein, dividing plate 3 is rectangular glass partition, and its size is identical with the level cross-sectionn size of hardening framework.Glass partition not only bearing is good, indeformable, and has light transmission, can make the tissue cultivating seedling that is placed in the seeding room 1 receive solar radiation.Dividing plate 3 is separated at least 2 layers with hardening framework 4, the space availability ratio height.
This embodiment median septum 3 is divided into 3 layers with hardening framework 4, hardening frame size length * wide * high (cm) is 120 * 60 * 180 (cm), every layer of appearance and size length * wide * high (cm) is: 120 * 60 * 60 (cm), promptly the size of seeding room 1 length * wide * high (cm) is: 120 * 60 * 60 (cm).
Hardening window 2 is a rectangular frame, and its length is identical with the width of seeding room 1, and its width is identical with the height of seeding room 1; Hardening window 2 is fixedly connected on the outside on hardening framework 4 relative two sides by top that is set in parallel in hardening framework 4 and the position rotating shaft 6 that hardening framework 4 is connected with dividing plate 3, promptly be installed in parallel in the front and back of hardening framework 4, therefore, a seeding room has two hardening windows, and promptly the quantity of hardening window is the twice of seeding room's 1 number.Hardening window 2 is positioned at the outside of the front and back of seeding room 1, is beneficial to the hardening window and opens laterally.
Clear glass is installed in the framework of hardening window 2, can transmission sunlight, make sunlight see through glass and shine on the test-tube plantlet that is placed in the seeding room 1.
When hardening window 2 was closed, seeding room 1 formed the space of sealing, and tool is by windproof, rainproof effect.Hardening window 2 rotates by rotating shaft 6, realizes its unlatching or closes the temperature and humidity in the adjusting sealing seeding room 1, the ventilation condition in the temperature in the hardening process of control tissue cultivating seedling, humidity and the seeding room 1.
Hardening window open system 7 is formed by supporting footstock 71, support slide bar 72, base for supporting 73, through hole 74, fixed knob 75, anti-slide plate 76 and handle 10, shown in Fig. 3,4,6,7,8.
Support the hemispherical that footstock 71 is hollow, be fixed on the side of hardening window 2 and and flexibly connect, an end that supports slide bar 72 is wrapped in its inside with an end that supports slide bar 72;
Shown in Figure 13 a, the inside of base for supporting 73 is two orthogonal through hole 74A, 74B, wherein through hole 74B is a cylindrical hole, the diameter of one end is greater than the diameter of the other end, the less end of through hole 74B diameter is installed in base for supporting 73 on the column 41 of hardening framework 4 sides by set bolt 11, and can serve as that axle rotates with set bolt 11, and the bigger end of cylindrical hole 74B diameter is away from the column 41 of hardening framework 4 sides.
There is one to rotate buffering spacer (not drawing among the figure) between on base for supporting 73 and the column 41, rotates easily to guarantee base for supporting.Base for supporting has into the through hole 74A of cuboid, supports slide bar 72 and passes through hole 74A, can be free to slide in through hole 74A.The top of the inherent support of the through hole 74A of base for supporting slide bar 72 is provided with anti-slide plate 76 along the glide direction that supports slide bar 72, along the glide direction that supports slide bar 72 rotation buffering spacer (not drawing among the figure) is set below supporting slide bar 72; And on anti-slide plate 76, fixed knob 75 is set, the diameter of fixed knob 75 is consistent with the diameter of the bigger end of diameter of through hole 74B, by screwing or unscrew fixed knob support slide bar 72 can be moved around in the through hole 74A on the base for supporting 73, the size that control hardening window 2 is opened, closed or open, the schematic diagram of hardening window opening device base for supporting 73, support slide bar, fixed knob is shown in Fig. 9 a, 9b, 9c.
The lower end of hardening window 2 is provided with handle 10, makes the unlatching of hardening window 2 or closes convenient.
The upper end of shading screen 8 is fixed on the top of hardening window 2, and it can be reeled or launch, and when solar radiation intensity was strong, shading screen launched, and weakens solar radiation intensity, and the size after shading screen 8 launches is identical with the size and the shape of hardening window 2 with shape; When the solar radiation weak strength, shading screen is wound on together, reel to the upper end from the lower end of shading screen 8, be fixed then.
Shading screen 8 can launch fully or be wound on fully together, also can be according to the solar radiation intensity shading screen that partly launches or reel, to satisfy the hardening needs of tissue cultivating seedling.
Be collected in the shading screen box 9 after shading screen 8 in the present embodiment is reeled, shading screen box 9 is installed in the top of hardening window 2 near rotating shaft 6, and is identical with the curtain and the pelmet of reeling, as shown in figure 10.
The shading screen of the utility model hardening device is fixed on the top of hardening window 2 as shading screen box 9 except using, can also be direct with the upper end fixed bonding of shading screen on the top of hardening window 2, its lower end can freely be rolled or launch; Fixedly connected with hardening window 2 after shading screen can also being made the form of shutter.
Shading screen box 9 is made up of the shell 91 that is fixed on hardening window 2 tops and the axostylus axostyle 92, the rope-winding wheel 93 that are positioned at shell 91, and axostylus axostyle 92 is installed on the fixed head 95 by bearing 94, and axostylus axostyle can freely rotate, and shading screen 8 is reeled or expansion is put down to drive.Axostylus axostyle 92 1 ends are equipped with rope-winding wheel 93, and rope-winding wheel has two races, and one of them is the forward wiring, and another is reverse wiring, by affecting around the rope 96 of taking turns, can drive axostylus axostyle 92 and rotate forward or backwards.Stage clip 97 is housed on rope-winding wheel 93 in addition, so both can be smooth around the rope of taking turns, and shading screen 8 is parked on the optional position.
Except installing the shading screen 8 on the hardening window 2, the shading screen 8 that can change all is installed at the side and the top thereof of hardening framework 4.
Shading screen can be changed, and by changing the shading screen of different shading rates, also can increase the number of plies of shading screen, to reach the purpose of regulation and control intensity of illumination.
Deflecting roller 5 is arranged on hardening framework 4 bottoms, have 4, be installed in hardening framework 4 bottoms four columns 41 below, stablizing when moving with assurance hardening device, deflecting roller 5 can be one-way wheel or universal wheel, the hardening device can be moved arbitrarily, thereby adjust the sensitive surface of tissue cultivating seedling, so that make the tissue cultivating seedling in the hardening device evenly accept to receive solar radiation.
A set bolt 51 is arranged on the deflecting roller 5, and when this set bolt was pressed, deflecting roller can not rotate, thereby the hardening device is maintained static; This set bolt is removed time-out, but the rotation of deflecting roller any direction, thus realize arbitrarily moving of hardening device.
Embodiment 2
Except hardening window open system 7 is made up of support ring 77, support bar 78, set bolt 79 and handle 10, shown in Figure 11,12.Wherein, described support ring 71 is fixed on hardening window 2 sides, is semi-annular shape; One end of support bar 78 is a hook-type, the other end is an annular, set bolt 79 passes the annulus end of support bar 78, support bar 78 is fixed on the column 41 of hardening framework 4 sides, and support bar 78 can serve as that axle rotates with set bolt 79, shown in Figure 13 a, 13b, when an end that is hook-type when support bar 78 is connected with support ring 77, when hook-type one end that is about to support bar 78 is socketed in the semicircular ring of support ring 77, hardening window 2 is opened; When hook-type one end with support bar 78 took out in the annulus of support ring 77, hardening window 2 was closed.
Some (3-5) circular support ring 77 is set in the side of hardening window 2, when opening hardening window 2, support bar 78 is socket-connected with different support ring 77, form different louver angles, ventilation, temperature adjustment, damping situation in the adjusting seeding room.
Except installing the shading screen 8 on the hardening window 2, the shading screen 8 that can change all is installed at the side and the top thereof of hardening framework 4.
Except above-mentioned difference, all the other are identical with embodiment 1.
It is as follows to adopt the step of utilizing hardening device of the present invention to carry out hardening:
1) the hardening device is shifted onto the tissue cultivating seedling raising chamber of taking root, turn is fixed on the fixed knob 75 on the base for supporting 73 on the column 41 of framework 4 sides, the support slide bar 72 that passes base for supporting 73 through holes can be moved freely, the handle 10 of pulling hardening window 2 bottoms is opened hardening window 2, when treating that hardening window 2 struts the degree of suitable placement test-tube plantlet, tighten fixed knob 75, will support that slide bar 72 compresses and fix by anti-slide plate 76, fixedly the hardening window 2;
2) tissue cultivating seedling that will take root is put on the glass partition 3 of seeding room 1 of hardening device, puts one deck rooting tube plantlet on every layer of dividing plate, does not interspace between bottle and the bottle, unclamps fixed knob 75, and hardening window 2 is closed, and seals seeding room 1;
3) the hardening device that the tissue cultivating seedling of taking root will be housed moves in the place of sunlight, carrying out hardening handles, wherein, adjust the unlatching of hardening window 2 or close or opening degree according to conditions such as hardening season, temperature, weather, if weather temperature height, sunny, intensity of illumination is big, the symmetrical hardening window 2 on both sides all need be opened, be ventilated, lower the temperature; If weather temperature is low, a little less than the sunlight, intensity of illumination is poor, then needs hardening window 2 is closed;
Generally speaking, it is opening that the hardening window 2 that need close seeding room 1 at night in early spring (March) or late fall (October), other times (April-September) can keep the hardening window evening, helps the hardening and the growth of tissue cultivating seedling.
4) after tissue cultivating seedling is handled 7-10 days through above-mentioned hardening,, continue then to carry out hardening by original condition with hardening device Rotate 180 degree;
5) after tissue cultivating seedling is handled 15-20 days through hardening, close hardening window 2, the hardening device is shifted onto in intermediate house or the booth, open hardening window 2, from seeding room 1, take out the seedling of taking root, carry out bottle outlet and transplant.

Claims (10)

1. the hardening device of a plant tissue culture seedlings-raising comprises:
Hardening framework (4), by cuboid or square that four root posts (41) are formed, its top, bottom and side are made up of the glass plate of light-permeable;
Dividing plate (3) is arranged on the hardening framework (4) from top to bottom, and hardening framework (4) is divided into the seeding room (1) that several place tissue cultivating seedling;
Hardening window (2) is installed in parallel in the front and back of described hardening framework (4).
2. hardening device as claimed in claim 1 is characterized in that also comprising shading screen (8), is fixed on hardening window (2) top.
3. hardening device as claimed in claim 1 or 2, it is characterized in that with the position that hardening framework (4) is connected with dividing plate (3) rotating shaft (6) being set at the top of hardening framework (4), hardening window (2) make described hardening window (2) be fixed on the outside of hardening framework (4), so that can be opened laterally by rotating shaft (6).
4. hardening device as claimed in claim 1 or 2 is characterized in that also comprising hardening window open system (7).
5. hardening device as claimed in claim 4 is characterized in that described hardening window open system comprises:
Support footstock (71), support slide bar (72) and have 2 base for supporting (73) of vertical and crossing through hole (74A, 74B) mutually, wherein
Support footstock (71) and be fixed on hardening window (2) side, be connected with the top of supporting slide bar (72);
Base for supporting (73) is fixed on the column (41) of hardening framework (4) side, and support litter (72) passes the through hole (74A) on the base for supporting (73), can move freely in base for supporting (73).
6. hardening device as claimed in claim 5, the through hole (74B) that it is characterized in that described base for supporting (73) is for cylindrical, and the diameter at its two ends is inequality.
7. hardening device as claimed in claim 6 is characterized in that setting gradually in the through hole (74B) of described base for supporting (73) slide bar fixed knob (75) from top to bottom and supports slide bar (72) and bolt (11), wherein,
Fixed knob (75) be pressed in support slide bar (72) above; Bolt (11) is fixed on base for supporting (73) on the column (41) of hardening framework.
8. hardening device as claimed in claim 4, it is characterized in that described hardening window open system comprises on the support ring that is semi-annular shape (77), support bar (78) that is fixed on hardening window (2) side and the column (41) that is fixed on hardening framework (4) side is circular support bar set bolt (79), wherein, support bar (78) is fixedlyed connected with set bolt (79).
9. hardening device as claimed in claim 1 or 2 is characterized in that also comprising the shading screen (8) that is installed in hardening framework (4) side.
10. hardening device as claimed in claim 1 or 2 is characterized in that also comprising the deflecting roller (5) of the bottom that is arranged on hardening framework (4).
CN2010206809939U 2010-12-24 2010-12-24 Plant tissue culture seedling exercising device Expired - Fee Related CN202014494U (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105072769A (en) * 2015-08-25 2015-11-18 华南理工大学 LED lighting device for simulating daylight movement in plant factory and LED lighting control method thereof
CN109775258A (en) * 2018-12-29 2019-05-21 铜陵天奇蓝天机械设备有限公司 A kind of driving tension vehicle having ceiling able to turn on or off
CN111109060A (en) * 2019-11-29 2020-05-08 南京农业大学 Method for increasing indoor domestication survival rate of strawberry virus-free seedlings
CN111727771A (en) * 2020-06-17 2020-10-02 安徽皖斛堂生物科技有限公司 Dendrobium officinale is planted with hardening seedling device
CN112493132A (en) * 2020-12-17 2021-03-16 刘泽洋 Method for improving gas microenvironment of plant tissue culture bottle seedlings and special culture medium

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105072769A (en) * 2015-08-25 2015-11-18 华南理工大学 LED lighting device for simulating daylight movement in plant factory and LED lighting control method thereof
CN105072769B (en) * 2015-08-25 2017-10-20 华南理工大学 The LED light device and its control method of simulating sun photomovement in plant factor
CN109775258A (en) * 2018-12-29 2019-05-21 铜陵天奇蓝天机械设备有限公司 A kind of driving tension vehicle having ceiling able to turn on or off
CN111109060A (en) * 2019-11-29 2020-05-08 南京农业大学 Method for increasing indoor domestication survival rate of strawberry virus-free seedlings
CN111727771A (en) * 2020-06-17 2020-10-02 安徽皖斛堂生物科技有限公司 Dendrobium officinale is planted with hardening seedling device
CN112493132A (en) * 2020-12-17 2021-03-16 刘泽洋 Method for improving gas microenvironment of plant tissue culture bottle seedlings and special culture medium

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