CN110809938B - Device and method for rapid germination of polygonatum sibiricum seeds - Google Patents
Device and method for rapid germination of polygonatum sibiricum seeds Download PDFInfo
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- CN110809938B CN110809938B CN201911128058.3A CN201911128058A CN110809938B CN 110809938 B CN110809938 B CN 110809938B CN 201911128058 A CN201911128058 A CN 201911128058A CN 110809938 B CN110809938 B CN 110809938B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a device and a method for rapid germination of polygonatum sibiricum seeds, wherein the device comprises a box body, a seed box and a seed box, wherein the box body is provided with a containing space, and a taking and placing opening is formed in the upper end or one side of the box body; the first driving plate is positioned in the accommodating space of the box body and is in sliding connection with the inner wall of the box body; the driving device is connected with the first driving plate and enables the first driving plate to move along the horizontal direction; the rotating assembly is positioned in the accommodating space of the box body and is fixedly connected with the box body, and the rotating assembly is rotationally connected with the first driving plate; the first drive plate drives the rotating assembly to continuously switch between positive rotation and reverse rotation, the rotating assembly comprises a mounting column fixedly connected with the bottom of the box body and a rotating shaft rotatably connected with the mounting column, a gear is fixedly arranged on the rotating shaft, one end, far away from the mounting column, of the rotating shaft is provided with a placing table, a cavity is formed in the drive plate, and a rack matched with the gear is arranged on the side wall of the cavity of the drive plate. The invention accelerates the germination efficiency of the polygonatum sibiricum seeds.
Description
Technical Field
The invention relates to the technical field of rhizoma polygonati seed germination, in particular to a device and a method for rapidly germinating rhizoma polygonati seeds.
Background
Rhizoma Polygonati is rhizome of Polygonatum kingianum Coll. et Hemsl, Polygonatum sibiricum Red. or Polygonatum cyrtonema Hua of Polygonatum of Liliaceae, and has effects of resisting aging, enhancing immunity, learning and memory reproduction ability, resisting inflammation, resisting bacteria, resisting virus, resisting tumor, reducing blood sugar and reducing blood lipid. The polygonatum kingianum is mainly produced in provinces such as Hebei, inner Mongolia and Shaanxi provinces, the polygonatum kingianum is mainly produced in provinces such as Guizhou, Yunnan and Guangxi, and the polygonatum cyrtonema is mainly produced in provinces such as Guizhou, Hunan and Yunnan. In recent years, with the gradual understanding of the medicinal value and the health care value of polygonatum by people, the increase of the market demand of polygonatum, the mass exploitation of wild polygonatum and the damage of the growth environment, the artificial planting is imperative, the propagation of polygonatum comprises sexual propagation and asexual propagation, and the polygonatum is mainly produced by the asexual propagation for a long time, so that the progeny degradation of polygonatum is extremely serious, and the yield and the quality of polygonatum medicinal materials are directly influenced. By using the sealwort seedling seed propagation, not only heterosis can be exerted, disease resistance and propagation coefficient are improved, but also quality degradation can be prevented, and the method is an effective way for solving the asexual virus propagation and preventing seed degradation of sealwort. However, the existing Polygonatum sibiricum seed germination method has the defects of low efficiency, complex operation and the like.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a device and a method for rapidly germinating polygonatum sibiricum seeds, and the germination efficiency of the polygonatum sibiricum seeds is accelerated.
The invention provides a device for quick germination of polygonatum sibiricum seeds, which comprises:
the box body is provided with an accommodating space, a taking and placing opening is formed in the upper end or one side of the box body, and a switch door is arranged at the taking and placing opening;
the first driving plate is positioned in the accommodating space of the box body and is in sliding connection with the inner wall of the box body;
the driving device is connected with the first driving plate and enables the first driving plate to move along the horizontal direction;
the rotating assembly is positioned in the accommodating space of the box body and is fixedly connected with the box body, and the rotating assembly is rotationally connected with the first driving plate;
the first driving plate drives the rotating assembly to continuously switch between forward rotation and reverse rotation.
Preferably, the runner assembly include with bottom half fixed connection's erection column, with the rotation axis of rotation that the erection column rotates to be connected, the fixed gear that is equipped with in the rotation axis, the rotation axis is kept away from the one end of erection column is equipped with places the platform, places the bench and is equipped with the buckle subassembly that is used for fixed blake bottle, this buckle subassembly adopt current technique can, only need the runner assembly when rotating the blake bottle can not the landing at will, be equipped with the cavity on the drive plate, the runner assembly is located the cavity, and be equipped with on the cavity lateral wall of drive plate with the rack that the gear matches.
Preferably, the bottom of the accommodation space of box is equipped with the reference column that is used for the fixed mounting post, reference column upper end opening and one side are equipped with the extension pipe, the extension pipe is equipped with the through-hole that communicates with the opening of reference column along the center pin direction, be equipped with the card strip in the through-hole, the cover is equipped with the spring on the card strip, the card strip is kept away from a pot head of reference column is equipped with the end cover, the end cover with card strip sliding connection, just the end cover with extension pipe threaded connection, the card strip is located the end cover is kept away from the one end detachably of reference column is equipped with the handle, be equipped with the draw-in groove that matches with the one end that the handle was kept away from to the card strip on the erection column, the shape of reference column is non-circular, can reduce the.
Preferably, the bottom of the accommodating space of the box body is provided with a positioning column for fixing the mounting column, and the positioning column and the mounting column are fixed through screws.
Preferably, a horizontal partition plate is further arranged in the accommodating space of the box body, a first drive plate and a plurality of rotating assemblies connected with the first drive plate are arranged in the accommodating space at the lower end of the partition plate, a second drive plate and a plurality of rotating assemblies connected with the second drive plate are arranged in the accommodating space at the upper end of the partition plate, a connecting rod is connected between the second drive plate and the first drive plate, the drive device is fixedly connected with the connecting rod, a sliding groove for sliding the connecting rod is formed in the partition plate, a taking and placing opening is formed in one side of each of the two accommodating spaces at the upper end and the lower end of the partition plate, and a switch door is arranged at the taking and.
The method for rapidly germinating the sealwort seeds by using the device provided by the invention comprises the following steps:
s1: sterilizing rhizoma Polygonati seeds, and placing into a culture flask containing liquid culture medium;
s2: fixing the culture bottle on the rotating assembly;
s3: the driving device is started to drive the first driving plate to move along the horizontal direction, the first driving plate drives the rotating assembly to continuously switch between positive rotation and reverse rotation, the driving device can be closed after seeds germinate, and the culture bottle can be taken down.
Preferably, the sterilization method in S1 is: washing seeds to remove epicarp, sterilizing with 75% alcohol for 30-60s, sterilizing with 8% NaClO for 10-15min, washing with sterile water for 4-5 times, sterilizing with 0.1% HgCl2 for 10-15min, washing with sterile water for 4-5 times, and air drying.
Preferably, the composition of the culture medium in S1 is: MS +1.0 mg.L-16-BA +0.5 mg.L-1 NAA +1.0 mg.L-1 GA 3.
Preferably, the rotation speed of the rotating assembly in the S3 is 130-170 r/min.
Compared with the prior art, the invention has the beneficial technical effects that: by adopting the device, the rotating direction of the culture medium is continuously switched in the process of rhizoma polygonati seed germination, so that the seed germination efficiency is improved; the invention can realize the rotating speeds of different culture media through one driving device, the rack arranged in the invention is detachably connected with the cavity, and the rotating speed of the rotating device can be changed by adjusting the size of the teeth on the rack and the gear, thereby realizing the purpose that one motor achieves a plurality of rotating speeds.
Drawings
FIG. 1 is a schematic structural view of a device for rapid germination of Polygonatum sibiricum seeds provided by the present invention;
FIG. 2 is a top view of a driving plate of the apparatus for rapid germination of Polygonatum sibiricum seeds provided by the present invention;
FIG. 3 is a cross-sectional view of a positioning column of the apparatus for rapid germination of Polygonatum sibiricum seeds provided by the present invention;
FIG. 4 is a schematic structural diagram of a device for rapid germination of Polygonatum sibiricum seeds according to another embodiment of the present invention;
FIG. 5 is a side sectional view of another embodiment of the apparatus for rapid germination of Polygonatum sibiricum seeds provided by the present invention.
In the figure: the device comprises a box body 1, a mounting column 2, a gear 3, a positioning column 4, a driving device 5, a cavity 6, a placing table 7, a rotating shaft 8, a first driving plate 9, a clamping groove 10, a through hole 11, an end cover 12, a handle 13, a spring 14, an extension tube 15, a clamping strip 16, a taking and placing opening 17, a rack 18, a partition plate 19, a connecting rod 20, a second driving plate 21 and a rotating assembly 22.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Referring to fig. 1-3, the device for rapid germination of polygonatum sibiricum seeds provided by the invention comprises:
the box body is provided with an accommodating space, a taking and placing opening is formed in the upper end or one side of the box body, and a switch door is arranged at the taking and placing opening;
the first driving plate is positioned in the accommodating space of the box body and is in sliding connection with the inner wall of the box body;
the driving device is connected with the first driving plate and enables the first driving plate to move along the horizontal direction;
the rotating assembly is positioned in the accommodating space of the box body and is fixedly connected with the box body, and the rotating assembly is rotationally connected with the first driving plate;
the rotating assembly comprises a mounting column fixedly connected with the bottom of the box body and a rotating shaft connected with the mounting column in a rotating mode, a gear is fixedly arranged on the rotating shaft, the rotating shaft is far away from one end of the mounting column and is provided with a placing table, a buckle assembly used for fixing a culture bottle is arranged on the placing table, the buckle assembly adopts the prior art, the culture bottle can not shake randomly when the rotating assembly rotates, a cavity is formed in the drive plate, the rotating assembly is located in the cavity, and a rack matched with the gear is arranged on the side wall of the cavity of the drive plate.
The axis of rotation with place detachably between the platform and be connected, only need take off the axis of rotation from placing the bench when the size of tooth on the needs adjustment gear, change can, need not to adjust placing the platform, reduce the cost.
The driving device drives the first driving plate to reciprocate along the horizontal direction, so that the first driving plate drives the rotating assembly to continuously switch between forward rotation and reverse rotation, and the purpose can be achieved through forward and reverse rotation of the motor.
The bottom of the accommodation space of box is equipped with the reference column that is used for the fixed mounting post, reference column upper end opening and one side are equipped with the extension pipe, the extension pipe is equipped with the through-hole that communicates with the opening of reference column along the center pin direction, be equipped with the card strip in the through-hole, the cover is equipped with the spring on the card strip, the card strip is kept away from a pot head of reference column is equipped with the end cover, the end cover with card strip sliding connection, just the end cover with extension pipe threaded connection, the card strip is located the end cover is kept away from the one end detachably of reference column is equipped with the handle, be equipped with the draw-in groove that matches with the one end that the handle was kept away from to the card strip on the erection column, the shape of reference column is non-circular, can.
Through the setting of the aforesaid to calorie strip structure, the installation of card strip has been made things convenient for, when the installation, lift the handle off from calorie strip earlier, then will block the strip and pass the end cover, then with handle and calorie strip fixed connection, and with the spring housing at the opposite side of calorie strip, then insert in the extension pipe with the card strip that the cover is equipped with the spring, and fix it on the extension pipe with the end cover can, the card strip is when initial condition, its one end of keeping away from the handle stretches into in the opening of reference column, when the erection column inserts in the reference column, the card strip is crowded outwards, draw-in groove and extension pipe on the erection column are when being right, the card strip stretches into in the draw-in groove under the effect of spring, thereby block the erection column, when needs lift the erection column off, only need manual outside pulling handle, make the card strip break away from the draw-in groove, can take off rotating assembly this moment.
The bottom of the accommodating space of the box body is provided with a positioning column for fixing the mounting column, and the positioning column is fixed with the mounting column through a screw.
Referring to fig. 4-5, for another embodiment of the present invention, a horizontally disposed partition board is further disposed in the accommodating space of the box body, a first driving board and a plurality of rotating assemblies connected to the first driving board are disposed in the accommodating space at the lower end of the partition board, a second driving board and a plurality of rotating assemblies connected to the second driving board are disposed in the accommodating space at the upper end of the partition board, a connecting rod is connected between the second driving board and the first driving board, the driving device is fixedly connected to the connecting rod, a sliding groove for sliding the connecting rod is disposed on the partition board, a taking and placing opening is disposed at one side of each of the two accommodating spaces at the upper end and the lower end of the partition board, and a switch door is disposed.
According to the invention, each driving plate can be provided with a plurality of cavities, and a plurality of rotating assemblies and positioning columns which are matched with the racks are arranged in the cavities, so that simultaneous culture of a plurality of culture mediums is realized, and the efficiency is improved.
Example 1
The method for rapidly germinating the sealwort seeds by using the device provided by the invention comprises the following steps:
s1: after washing to remove epicarp, sterilizing with 75% alcohol for 30s on a clean bench, sterilizing with 8% NaClO for 10min, washing with sterile water for 4 times, sterilizing with 0.1% HgCl2 for 10min, washing with sterile water for 4 times, air drying in the clean bench, and placing into a culture medium composed of MS +1.0 mg.L-16-BA +0.5 mg.L-1 NAA +1.0 mg.L-1 GA 3;
s2: fixing the culture medium on a rotating assembly;
s3: and starting the driving device to drive the first driving plate to move along the horizontal direction, driving the rotating assembly to continuously switch between positive rotation and reverse rotation by the first driving plate, wherein the rotating speed of the rotating assembly is 130r/min, and closing the driving device after the seeds germinate, and taking down the culture medium. The starting time of seed germination of the scheme is 8 days, and the germination rate is 78% after 20 days.
Example 2
The method for rapidly germinating the sealwort seeds by using the device provided by the invention comprises the following steps:
s1: after being washed clean and the epicarp is removed, the cell is firstly disinfected by 75 percent alcohol for 60s on a clean bench, then disinfected by 8 percent NaClO for 15min, washed by sterile water for 5 times, and finally disinfected by 0.1 percent HgCl2 for 15min, washed by sterile water for 5 times, and placed into a culture medium consisting of MS +1.0 mg.L-16-BA +0.5 mg.L-1 NAA +1.0 mg.L-1 GA3 after being dried in the air;
s2: fixing the culture medium on a rotating assembly;
s3: and starting the driving device to drive the first driving plate to move along the horizontal direction, driving the rotating assembly to continuously switch between positive rotation and reverse rotation by the first driving plate, wherein the rotating speed of the rotating assembly is 170r/min, and closing the driving device after the seeds germinate, and taking down the culture medium. The starting time of seed germination of the scheme is 7 days, and the germination rate is 84% after 20 days.
Example 3
The method for rapidly germinating the sealwort seeds by using the device provided by the invention comprises the following steps:
s1: after being washed clean and the epicarp is removed, the cell is firstly disinfected by 75 percent alcohol for 45s on a clean bench, then disinfected by 8 percent NaClO for 12min, washed by sterile water for 4 times, and finally disinfected by 0.1 percent HgCl2 for 12min, washed by sterile water for 4 times, and placed into a culture medium consisting of MS +1.0 mg.L-16-BA +0.5 mg.L-1 NAA +1.0 mg.L-1 GA3 after being dried in the air;
s2: fixing the culture medium on a rotating assembly;
s3: and starting the driving device to drive the first driving plate to move along the horizontal direction, driving the rotating assembly to continuously switch between positive rotation and reverse rotation by the first driving plate, wherein the rotating speed of the rotating assembly is 150r/min, and closing the driving device after the seeds germinate, and taking down the culture medium. The starting time of seed germination of the scheme is 5 days, and the germination rate is 89% after 20 days.
Comparative example 1
S1: after washing to remove epicarp, sterilizing with 75% alcohol for 30s on a clean bench, sterilizing with 8% NaClO for 10min, washing with sterile water for 4 times, sterilizing with 0.1% HgCl2 for 10min, washing with sterile water for 4 times, air drying in the clean bench, and placing into a culture medium composed of MS +1.0 mg.L-16-BA +0.5 mg.L-1 NAA +1.0 mg.L-1 GA 3;
s2: fixing the culture medium on a shaking table with model ZQPL-200, setting the rotation speed at 130r/min, closing the shaking table after the seeds germinate, and taking down the culture medium. The starting time of seed germination of the scheme is 15 days, and the germination rate is 44% after 20 days.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. Device that sealwort seed was sprouted fast, its characterized in that includes:
the box body is provided with an accommodating space, and the upper end or one side of the box body is provided with a taking and placing opening;
the first driving plate is positioned in the accommodating space of the box body and is in sliding connection with the inner wall of the box body;
the driving device is connected with the first driving plate and enables the first driving plate to move along the horizontal direction;
the rotating assembly is positioned in the accommodating space of the box body and is fixedly connected with the box body, and the rotating assembly is rotationally connected with the first driving plate;
the first driving plate drives the rotating assembly to continuously switch between positive rotation and negative rotation;
the rotating assembly comprises a mounting column fixedly connected with the bottom of the box body and a rotating shaft rotatably connected with the mounting column, a gear is fixedly arranged on the rotating shaft, a placing table is arranged at one end, away from the mounting column, of the rotating shaft, a cavity is formed in the driving plate, the rotating assembly is located in the cavity, and a rack matched with the gear is arranged on the side wall of the cavity of the driving plate;
the bottom of the accommodation space of box is equipped with the reference column that is used for the fixed mounting post, reference column upper end opening and one side are equipped with the extension pipe, the extension pipe is equipped with the through-hole that communicates with the opening of reference column along the center pin direction, be equipped with the card strip in the through-hole, the cover is equipped with the spring on the card strip, the card strip is kept away from a pot head of reference column is equipped with the end cover, the end cover with card strip sliding connection, just the end cover with extension pipe threaded connection, the card strip is located the end cover is kept away from the one end detachably of reference column is equipped with the handle, be equipped with the draw-in groove that matches with the one end that.
2. The device for rapidly germinating rhizoma polygonati seeds according to claim 1, wherein a positioning column for fixing the mounting column is arranged at the bottom of the accommodating space of the box body, and the positioning column and the mounting column are fixed through screws.
3. The device for rapidly germinating rhizoma polygonati seeds according to claim 1, wherein a horizontally arranged partition plate is further arranged in the accommodating space of the box body, a first drive plate and a plurality of rotating assemblies connected with the first drive plate are arranged in the accommodating space at the lower end of the partition plate, a second drive plate and a plurality of rotating assemblies connected with the second drive plate are arranged in the accommodating space at the upper end of the partition plate, a connecting rod is connected between the second drive plate and the first drive plate, the drive device is fixedly connected with the connecting rod, and a sliding groove for sliding of the connecting rod is formed in the partition plate.
4. A method for rapidly germinating sealwort seeds by adopting the device of any one of claims 1 to 3, which is characterized by comprising the following steps:
s1: sterilizing rhizoma Polygonati seeds, and placing into a culture flask containing liquid culture medium;
s2: fixing the culture bottle on the rotating assembly;
s3: the driving device is started to drive the first driving plate to move along the horizontal direction, the first driving plate drives the rotating assembly to continuously switch between positive rotation and reverse rotation, the driving device can be closed after seeds germinate, and the culture bottle can be taken down.
5. The method for rapidly germinating the sealwort seeds according to claim 4, wherein the sterilization method in S1 is as follows: cleaning seeds, removing epicarp, sterilizing with 75% ethanol for 30-60s, sterilizing with 8% NaClO for 10-15min, washing with sterile water for 4-5 times, sterilizing with 0.1% HgCl2 for 10-15min, washing with sterile water for 4-5 times, and air drying.
6. The method for rapid germination of polygonatum sibiricum seeds according to claim 4, wherein the composition of the culture medium in S1 is as follows: MS +1.0 mg.L-16-BA +0.5 mg.L-1 NAA +1.0 mg.L-1 GA 3.
7. The method for rapidly germinating rhizoma Polygonati seeds as claimed in claim 4, wherein the rotation speed of the rotation assembly in S3 is 130-170 r/min.
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| CN201911128058.3A CN110809938B (en) | 2019-11-18 | 2019-11-18 | Device and method for rapid germination of polygonatum sibiricum seeds |
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| CN201911128058.3A CN110809938B (en) | 2019-11-18 | 2019-11-18 | Device and method for rapid germination of polygonatum sibiricum seeds |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114223345A (en) * | 2020-09-09 | 2022-03-25 | 胡士艳 | Efficient seed soaking and germination accelerating device for agricultural planting |
| CN115589949A (en) * | 2022-11-24 | 2023-01-13 | 江西师范大学（Cn） | Tissue culture method for one-step seedling formation and propagation of polygonatum cyrtonema seeds |
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