CN107955756B - Handmade soap and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of washing and caring articles, and particularly relates to handmade soap and a preparation method thereof. The handmade soap comprises the following components in parts by weight: 6-9 parts of sodium hydroxide, 20-40 parts of grease, 0.5-2 parts of citronella essential oil, 4-6 parts of emollient, 5-8 parts of ant extract and 30-42 parts of deionized water. The handmade soap provided by the invention is simple in preparation method, good in cleaning effect, small in skin irritation, not tight after washing, and capable of relieving itching, resisting inflammation, whitening and removing scars, and can be stored for a long time.

Description

Handmade soap and preparation method thereof

Technical Field

The invention belongs to the technical field of washing and caring articles, and particularly relates to handmade soap and a preparation method thereof.

Background

in recent years, a handmade soap is gradually created, which is prepared by saponification of natural oil and alkali, a large amount of glycerin is generated in the saponification process, the glycerin is the best natural humectant for skin, a layer of natural protective film is formed on the skin, and in addition, various additives such as cow milk, breast milk, soybean milk, essential oil, essence, flowers and plants, traditional Chinese medicinal materials, bamboo charcoal powder, preservatives, dyes and the like can be added into the handmade soap according to personal preference and purposes, so that different effects are achieved.

chinese patent application CN102433228A discloses a red wine soap and a preparation method thereof, wherein the red wine soap is prepared from red wine, edible oil, plant care oil, purified water, honey, transparent soap base and grape seed essential oil, and the red wine soap has the effects of decontamination, deep oxidation resistance, whitening and moisture retention, but the red wine itself has certain irritation to the skin and damages the skin, and the preparation process of the raw material soap base usually needs to add alcohol or other substances to make the soap base transparent, but the irritation of the alcohol to the skin is also well known.

Chinese patent application CN106085661A discloses an anti-mosquito handmade soap and a manufacturing method thereof, the handmade soap is added with raw materials such as lavender petals and michelia mangiflora petals, when the handmade soap is used for cleaning skin, various nutritional ingredients such as vegetable oil and fat beneficial to skin can be left on skin, and drugs with an anti-mosquito effect can be kept, however, free alkali which does not completely react often exists in the finished handmade soap, the added petals can be corroded by alkali, and even the added petals can change color or become black after being placed for a long time, on the contrary, the soap is polluted, and the storage life is short.

The Chinese patent application CN104263560A discloses a scar-removing perfumed soap which has the scar-removing effect on the basis of basic functions of decontamination and whitening, is suitable for the skin hurt people, but if the perfumed soap is used for a long time, the contained essence component can cause certain damage to the nervous system and the hematopoietic system.

With the development of society, the requirements of people on daily necessities are higher and higher, the requirements of people on handmade soaps are not only in the aspect of cleaning, but also the soap is expected to have the functions of whitening, removing scars, resisting aging and the like so as to improve the quality of life, and meanwhile, the irritation to the skin, the storage life and the like are problems which need to be avoided, so that the handmade soap which has good cleaning effect, has the effects of whitening, moisturizing, removing scars and the like, and is small in irritation to the skin and stable in property is needed to be developed.

Disclosure of Invention

in order to solve the problems in the prior art, the invention aims to provide a handmade soap and a preparation method thereof. The handmade soap provided by the invention is simple in preparation method, good in cleaning effect, mild in property, free of irritation and not tight after washing, has the effects of relieving itching, resisting inflammation, removing scars and whitening, and can be stored for a long time.

The invention provides handmade soap which comprises the following components in parts by weight:

6-9 parts of sodium hydroxide, 20-40 parts of grease, 0.5-2 parts of citronella essential oil, 4-6 parts of emollient, 5-8 parts of ant extract and 30-42 parts of deionized water.

Preferably, the handmade soap consists of the following components in parts by weight:

8 parts of sodium hydroxide, 30 parts of grease, 1.5 parts of citronella essential oil, 5 parts of emollient, 7 parts of ant extract and 36 parts of deionized water.

preferably, the grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1 (1-5) to (4-8).

The skin lotion comprises squalane, the squalane is animal oil with high chemical stability, has certain affinity to the skin, helps to maintain the balance between sebum of the skin, has obvious effects of delaying skin aging, improving and eliminating chloasma, and although the squalane can accelerate the permeation of active ingredients into the skin, the active ingredients with the functions of whitening, resisting oxidation and the like can be absorbed by the horny layer and then permeate other layers of the epidermis to play a role only when the skin has sufficient moisture and can soften the horny layer.

the chitosan oligosaccharide can inhibit bacteria on the surface of the skin, activate epidermal cells and enhance the elasticity of the skin, in addition, the molecules of the chitosan oligosaccharide contain hydroxyl, amino and other groups with good affinity with water molecules, partial water molecules can be retained, the chitosan oligosaccharide has moisturizing performance, and can be used as a moisturizing agent in cosmetics, but the chitosan oligosaccharide cannot achieve ideal moisturizing effect when being used in a small amount or used alone.

The essential oil contains polar substances as main ingredients, has small molecular weight and high volatility, has natural fragrance, contains no waxy component, and has antibacterial, hemostatic and antiallergic effects.

The inventor unexpectedly finds that the combination of squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil achieves unexpected technical effects.

The combination of the components not only greatly improves the moisturizing performance of the handmade soap, improves the absorption rate of skin to active ingredients, but also has a certain scar removing effect, preferably, the emollient is prepared from squalane, chitosan oligosaccharide and argy wormwood leaf essential oil in a weight ratio of 1: (2-4) and (3-6).

The squalane, the chitosan oligosaccharide and the folium artemisiae argyi essential oil have a synergistic effect, the squalane can remarkably increase the skin permeation amount of the chitosan oligosaccharide and the folium artemisiae argyi essential oil, the transdermal permeation rate is increased, the bioavailability and the effectiveness of the chitosan oligosaccharide are remarkably improved, meanwhile, the good moisturizing performance of the chitosan oligosaccharide remarkably improves the absorption promoting effect of the squalane on active ingredients in the formula, and the squalane, the chitosan oligosaccharide and the folium artemisiae argyi essential oil complement each other to jointly achieve the effects of moisturizing and protecting the skin. In addition, the combination use of squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil also enables the handmade soap to have excellent scar removing effect, wherein the total scar removing effective rate is as high as 93%.

but not all proportions of squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil can achieve synergistic effect, and preferably, the emollient is composed of squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil according to the weight ratio of 1:3: 5.

Preferably, the preparation method of the ant extract comprises the following steps:

(1) Drying red ants, grinding in a ball mill, and sieving to obtain ant powder;

(2) adding the ant powder obtained in the step (1) into a sodium bicarbonate solution with the mass concentration of 3-5%, stirring for 20-30min, filtering, and drying to obtain pre-treated ant powder;

(3) Adding rice wine 5-8 times the weight of the pretreated ant powder obtained in step (2), performing ultrasonic extraction at 40-52 deg.C for 40-90min, filtering, concentrating, and drying.

Preferably, the adding ratio of the ant powder and the sodium bicarbonate solution in the step (2) is 1 g: 5-10 mL.

Preferably, the alcohol content of the rice wine in the step (3) is 15-20 degrees.

in addition, the invention also provides a preparation method of the handmade soap, which comprises the following steps:

S1, dissolving sodium hydroxide in deionized water, and cooling to 45-50 ℃ to obtain alkali liquor;

S2, uniformly mixing the grease, and heating to 45-53 ℃ to obtain mixed grease;

S3, slowly adding the alkali liquor obtained in the step S1 into the mixed oil obtained in the step S2, stirring while adding, obtaining a viscous soap solution after 1-2 hours, then adding the citronella essential oil, the emollient and the ant extract into the soap solution, stirring uniformly, pouring into a mold, standing at normal temperature for 24-40 hours, and demolding to obtain a molded solid;

s4, placing the molded solid obtained in the step S3 in a cool and ventilated place for curing for 30 days, and obtaining the product.

The invention adopts pure natural vegetable oil, the vegetable oil contains a large amount of nutrient substances which do not participate in saponification, and the components can permeate into skin through pores when in use, thereby achieving the effect of cleaning and maintaining simultaneously. The ant powder is pretreated to remove formic acid which is corrosive to skin, pollution of organic solvents in the traditional method is avoided by adopting rice wine ultrasonic extraction, the obtained ant extract simultaneously contains amino acids, mineral substances, vitamins and other components in ants and rice wine, and the inventor also finds that the ant powder can inhibit the activity of tyrosinase, has a certain whitening effect, can reduce the generation of active oxygen and has a certain antioxidation effect.

Compared with the prior art, the invention has the technical advantages that:

(1) The handmade soap does not contain components which have irritation to skin such as surfactant and the like, has mild property and is not tight after washing; (2) the handmade soap has reasonable and scientific compatibility of all components, has the effects of relieving itching and resisting inflammation, has better affinity to skin, good moisturizing performance and scar removing effect by using squalane, chitosan oligosaccharide and argy wormwood leaf essential oil in the skin moisturizer in a combined way, and has good whitening effect by using the ant extract; (3) the handmade soap can be used for a long time, and has stable property and long storage life.

Detailed Description

The present invention is further illustrated by the following specific examples, which are provided for illustrative purposes only and do not limit the scope of the present invention.

in the invention, the coconut oil, shea butter and cocoa butter are all purchased from Jiangxi Anlun natural spice, Inc.; rice wine was purchased from Yangchunxin de wine industry Co., Ltd; citronella essential oil CAS no: 73049-78-2; folium artemisiae argyi essential oil CAS no: 8008-93-3; chitosan oligosaccharide CAS No.: 148411-57-8; squalane CAS number: 111-01-3.

example 1A handmade soap

The handmade soap comprises the following components in parts by weight:

6 parts of sodium hydroxide, 20 parts of grease, 2 parts of citronella essential oil, 4 parts of emollient, 8 parts of ant extract and 30 parts of deionized water; the grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1:1: 8; the skin lotion is prepared from squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil according to the weight ratio of 1:2: 3.

The preparation method of the ant extract comprises the following steps:

(1) Drying red ants, grinding in a ball mill, and sieving to obtain ant powder;

(2) Adding the ant powder obtained in the step (1) into a sodium bicarbonate solution with the mass concentration of 5%, wherein the adding ratio of the ant powder to the sodium bicarbonate solution is 1 g: 5mL, stirring for 20min, filtering and drying to obtain pretreated ant powder;

(3) Adding 5 times of rice wine with alcohol content of 15% into the pretreated ant powder obtained in step (2), ultrasonically extracting at 52 deg.C for 90min, filtering, concentrating, and drying.

The preparation method comprises the following steps:

S1, dissolving sodium hydroxide in deionized water, and cooling to 45 ℃ to obtain alkali liquor;

s2, uniformly mixing the grease, and heating to 45 ℃ to obtain mixed grease;

S3, slowly adding the alkali liquor obtained in the step S1 into the mixed oil obtained in the step S2, stirring while adding, obtaining a viscous soap solution after 1 hour, adding the citronella essential oil, the emollient and the ant extract into the soap solution, uniformly stirring, pouring into a mold, standing at normal temperature for 24 hours, and demolding to obtain a molded solid;

S4, placing the molded solid obtained in the step S3 in a cool and ventilated place for 30 days to obtain the product.

Example 2A handmade soap

The handmade soap comprises the following components in parts by weight:

8 parts of sodium hydroxide, 30 parts of grease, 1.5 parts of citronella essential oil, 5 parts of emollient, 7 parts of ant extract and 36 parts of deionized water; the grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1:3: 6; the skin lotion is prepared from squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil according to the weight ratio of 1:3: 5.

The preparation method of the ant extract comprises the following steps:

(1) Drying red ants, grinding in a ball mill, and sieving to obtain ant powder;

(2) Adding the ant powder obtained in the step (1) into a sodium bicarbonate solution with the mass concentration of 4%, wherein the adding ratio of the ant powder to the sodium bicarbonate solution is 1 g: stirring for 25min by 8mL, filtering, and drying to obtain pretreated ant powder;

(3) Adding 7 times of rice wine with alcohol content of 18% into the pretreated ant powder obtained in step (2), ultrasonic extracting at 46 deg.C for 70min, filtering, concentrating, and drying.

The preparation method comprises the following steps:

S1, dissolving sodium hydroxide in deionized water, and cooling to 45 ℃ to obtain alkali liquor;

S2, uniformly mixing the grease, and heating to 48 ℃ to obtain mixed grease;

S3, slowly adding the alkali liquor obtained in the step S1 into the mixed oil obtained in the step S2, stirring while adding, obtaining a viscous soap solution after 1.5h, then adding the citronella essential oil, the emollient and the ant extract into the soap solution, stirring uniformly, pouring into a mold, standing at normal temperature for 32h, and demolding to obtain a molded solid;

S4, placing the molded solid obtained in the step S3 in a cool and ventilated place for 30 days to obtain the product.

Example 3A handmade soap

the handmade soap comprises the following components in parts by weight:

9 parts of sodium hydroxide, 40 parts of grease, 0.5 part of citronella essential oil, 6 parts of emollient, 5 parts of ant extract and 42 parts of deionized water; the grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1:5: 4; the skin lotion is prepared from squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil according to the weight ratio of 1:4: 6.

the preparation method of the ant extract comprises the following steps:

(1) drying red ants, grinding in a ball mill, and sieving to obtain ant powder;

(2) Adding the ant powder obtained in the step (1) into a sodium bicarbonate solution with the mass concentration of 3%, wherein the adding ratio of the ant powder to the sodium bicarbonate solution is 1 g: stirring for 30min by 10mL, filtering, and drying to obtain pretreated ant powder;

(3) Adding 8 times of rice wine with alcohol content of 20% into the pretreated ant powder obtained in step (2), performing ultrasonic extraction at 40 deg.C for 40min, filtering, concentrating, and drying.

The preparation method comprises the following steps:

S1, dissolving sodium hydroxide in deionized water, and cooling to 50 ℃ to obtain alkali liquor;

s2, uniformly mixing the grease, and heating to 53 ℃ to obtain mixed grease;

s3, slowly adding the alkali liquor obtained in the step S1 into the mixed oil obtained in the step S2, stirring while adding, obtaining a viscous soap solution after 2 hours, adding citronella essential oil, an emollient and an ant extract into the soap solution, stirring uniformly, pouring into a mold, standing at normal temperature for 40 hours, and demolding to obtain a molded solid;

S4, placing the molded solid obtained in the step S3 in a cool and ventilated place for 30 days to obtain the product.

comparative example 1, a handmade soap

The handmade soap comprises the following components in parts by weight:

8 parts of sodium hydroxide, 30 parts of grease, 1.5 parts of citronella essential oil, 5 parts of emollient, 7 parts of ant extract and 36 parts of deionized water; the grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1:3: 6; the skin lotion is prepared from squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil according to the weight ratio of 1:1: 1.

The preparation method of ant extract is similar to that of example 2.

the preparation method is similar to example 2.

The difference from example 2 is that the emollient consists of squalane, chitosan oligosaccharide and mugwort leaf essential oil in a weight ratio of 1:1: 1.

Comparative example 2 a handmade soap

The handmade soap comprises the following components in parts by weight:

8 parts of sodium hydroxide, 30 parts of grease, 1.5 parts of citronella essential oil, 5 parts of emollient, 7 parts of ant extract and 36 parts of deionized water; the grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1:3: 6; the emollient is composed of squalane, propylene glycol and folium artemisiae argyi essential oil according to the weight ratio of 1:3: 5.

the preparation method of ant extract is similar to that of example 2.

the preparation method is similar to example 2.

The difference from example 2 is that the chitosan oligosaccharide was replaced by propylene glycol, i.e. the emollient consisted of squalane, propylene glycol and essential oil of argy wormwood leaf in a weight ratio of 1:3: 5.

Comparative example 3 a handmade soap

The handmade soap comprises the following components in parts by weight:

8 parts of sodium hydroxide, 30 parts of grease, 1.5 parts of citronella essential oil, 5 parts of emollient, 7 parts of ant extract and 36 parts of deionized water; the grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1:3: 6; the emollient is composed of squalane and chitosan oligosaccharide in a weight ratio of 1: 8.

The preparation method of ant extract is similar to that of example 2.

The preparation method is similar to example 2.

The difference from example 2 is that no essential oil of argy wormwood leaves was added, i.e. the emollient consists of squalane and chitosan oligosaccharide in a weight ratio of 1: 8.

Comparative example 4 a handmade soap

The handmade soap comprises the following components in parts by weight:

8 parts of sodium hydroxide, 30 parts of grease, 1.5 parts of citronella essential oil, 5 parts of emollient, 7 parts of ant extract and 36 parts of deionized water; the grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1:3: 6; the skin lotion is prepared from squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil according to the weight ratio of 1:3: 5.

The preparation method of the ant extract comprises the steps of replacing the rice wine in the step (3) with 70% ethanol by volume, and the rest steps are the same as the steps in the example 2.

The preparation method is similar to example 2.

The difference from example 2 is that the rice wine was replaced with 70 vol% ethanol in the preparation method of ant extract.

Test example I, user experience testing

1. Test subjects: 70 healthy volunteers (35 each for male and female) aged 20-30 years.

2. test materials: handmade soaps prepared according to examples 1-3 of the present invention and comparative examples 1-4.

3. The test method comprises the following steps:

70 subjects were randomly divided into 7 groups, and the medial side of the arm to which the BB cream was applied was washed once every 8 hours for 2 times per day for four weeks using the handmade soaps prepared in examples 1 to 3 and comparative examples 1 to 4. The tested person scores the cleanness, the irritation and the foam performance of the skin after use (0-10 points, the higher the score is, the better the cleaning effect is, the less irritation to the skin, the rich foam and the better fineness are).

4. and (3) test results: the test results are shown in table 1.

TABLE 1 user experience test results

Group of	example 1	Example 2	Example 3	Comparative example 1	Comparative example 2	comparative example3	Comparative example 4
								Cleanliness	8.2	8.6	8.3	7.4	7.1	7.3	7.5
Irritation property	9.3	9.8	9.6	8.4	8.5	8.1	8.2
								Foam performance	8.8	9.5	8.7	8.5	8.2	7.9	8.1

As can be seen from Table 1, the handmade soap prepared by the invention has better using effect and does not cause adverse skin reactions such as edema and allergy of a subject. Compared with comparative examples 1-4, the handmade soaps prepared in examples 1-3 of the present invention have strong cleansing effect, fine and rich foam, and small irritation to skin, wherein the user experience effect of example 2 is the best example of the present invention.

test example two, antipruritic and anti-inflammatory test

1. Test subjects: SPF KM mice 160, half male and female, weight 18-22 g.

2. Test materials: handmade soaps prepared according to examples 1-3 of the present invention and comparative examples 1-4.

3. The test method comprises the following steps:

(1) Itching relieving test: 80 mice were randomly divided into 8 groups, i.e., examples 1-3 groups, comparative examples 1-4 groups, and a blank control group. The mice were cleaned after their backs had been depilated, the blank group was cleaned with normal saline, the other groups were cleaned with handmade soap prepared from the corresponding group, and the ratio of the body weight of the mice to the amount of handmade soap/normal saline was 1:0.1, 2 times/day, for 7 consecutive days. After 30min of the last washing, dextran (mw ═ 40000) injected into tail vein of each group of mice at 1.25mg/kg (0.025%, 0.05ml/10g) resulted in an animal model of paroxysmal skin itch. The head of the mouse is grabbed by the front paw, the trunk of the mouse is scratched by the back paw, and all parts of the whole body are bitten by the mouth to serve as pruritus indications. The number of itching episodic episodes and the duration of the episodes were recorded in the mice over 30 min.

(2) Anti-inflammatory assay: 80 mice were randomly divided into 8 groups, i.e., examples 1-3 groups, comparative examples 1-4 groups, and a blank control group. The mouse was cleaned after depilating the back, the blank group was cleaned with normal saline, the other groups were cleaned with handmade soap prepared from the corresponding group, the weight of the mouse and the handmade soap/normal saline mass ratio were 1:0.1, 2 times/day, and 7 consecutive days. After 30min of the last washing, the left ear of each mouse was coated with 0.02ml of 100% xylene on both sides to cause inflammation, and the right ear was used as a control. The animals were sacrificed 2h after the inflammation, both ears were cut along the auricle base line, a wafer of the same part of both ears was punched out with a puncher with a diameter of 9mm, weighed with an electronic analytical balance, and the ear weight difference was calculated: the difference of the ear weight is the weight of the left ear piece to the weight of the right ear piece; the swelling inhibition (%) was ═ weight difference between blank group and handmade soap group/weight difference between blank group x 100%.

4. And (3) test results: the test results are shown in tables 2 and 3.

TABLE 2 mouse antipruritic test results

Group of	N (only)	Number of itching (n)	Duration of pruritus(s)
				Blank control	10	23.7±1.9	402.6±25.2
Example 1	10	12.1±2.3	234.7±41.3
				Example 2	10	10.6±3.7	208.9±32.7
Example 3	10	13.3±2.5	210.5±45.4
				Comparative example 1	10	16.5±4.6	294.4±61.2
Comparative example 2	10	17.2±3.9	272.3±49.5
				Comparative example 3	10	16.3±4.1	293.9±54.8
Comparative example 4	10	15.7±2.3	324.6±43.6

TABLE 3 anti-inflammatory test results in mice

As can be seen from tables 2 and 3, the handmade soap prepared by the invention has good itching relieving and anti-inflammatory effects, can reduce the frequency and duration of paroxysmal pruritus of mice, and can inhibit the degree of ear swelling of the mice, wherein the itching relieving and anti-inflammatory effects of the example 2 are best, the swelling inhibition rate is 62.9%, and the best example of the invention shows that the handmade soap has reasonable and scientific compatibility of components and good itching relieving and anti-inflammatory effects.

Test example III inhibition of tyrosinase Activity test

the color of the skin is related to the type and the amount of pigments in the skin, wherein the influence of melanin on the color of the skin is the largest, and tyrosinase plays the most important role in the formation process of the melanin, so that the effect of whitening can be achieved by inhibiting the activity of the tyrosinase.

1. Test materials: handmade soaps prepared according to examples 1-3 of the present invention and comparative examples 1-4.

2. The test method comprises the following steps:

taking four 10mL volumetric flasks, marked as A1, A2, B1 and B2 respectively, adding 5mL of phosphoric acid buffer solution with the pH value of 6.8 into the four volumetric flasks, adding 2g of the handmade soap chips prepared by the method into the flasks B1 and B2, and placing the flasks in a water bath at 37 ℃ for keeping the temperature for 10 minutes; then 0.5mL of tyrosinase solution was added to each of A2 and B2, and finally a phosphate buffer solution was added to the solution to a constant volume, and after 5 minutes of reaction, the reaction mixture was quickly transferred to a cuvette and absorbance at 475nm was measured using an ultraviolet-spectrophotometer.

The inhibition rate was [1- (B2-B1)/(a2-a1) ] × 100%.

3. and (3) test results: the test results are shown in table 4.

TABLE 4 results of tyrosinase activity inhibition assay

As can be seen from Table 4, the handmade soaps prepared in examples 1-3 and comparative examples 1-4 each had various degrees of inhibitory effect on tyrosinase. The inhibition rates of the handmade soaps in the examples 1 to 3 are all above 70%, wherein the inhibition effect of the handmade soap in the example 2 on tyrosinase is strongest and reaches 76.2%, the whitening effect is best, and the inhibition rate of the comparative example 4 is lowest, which shows that the ant extract obtained by ultrasonic extraction of the rice wine has a better whitening effect on skin.

Test example four, moisture retention Performance test

1. test subjects: 70 healthy women aged 20-45 years, dry skin, and the subjects excluded any factors that may affect the experimental results, such as certain diseases, allergy to certain cosmetics or skin care products and other external preparations.

2. Test materials: handmade soaps prepared according to examples 1-3 of the present invention and comparative examples 1-3.

3. The test method comprises the following steps:

The skin moisture content was measured using a skin moisture meter. The higher the value, the higher the skin moisture content.

the subjects were divided into 7 groups at random, and 2 areas of 5cm × 5cm on the forearm extension of the subjects were selected as the test sites and the control sites. The test subject washed the test site and the control site with water, and the skin moisture meter was used to measure the basal moisture content of the skin. After 5h, the control site was washed with water, the test site was washed with a hand soap, and the skin moisture content was again measured after 1, 2, 4, and 6 h. 4. And (3) test results: the test results are shown in table 5.

Table 5 skin moisture test results

Categories	Foundation	1 hour	2 hours	4 hours	6 hours
						Blank control	35.37	34.72	32.59	32.08	30.92
Example 1	34.62	58.25	56.14	52.39	48.73
						example 2	35.30	65.53	63.89	60.42	57.69
Example 3	36.41	62.36	60.18	55.71	51.38
						comparative example 1	35.46	54.13	49.26	46.59	42.08
comparative example 2	35.53	53.72	48.56	43.28	39.57
						Comparative example 3	36.28	57.31	50.14	45.62	40.35

as can be seen from Table 5, the handmade soaps prepared in examples 1-3 of the present invention had better moisturizing properties, wherein the best results of example 2 are shown, and the skin moisture content after 6 hours of washing is 1.9 times that of the blank control group and 1.5 times that of comparative example 2, which indicates that squalane, chitosan oligosaccharide and argy wormwood leaf essential oil in the formulation of the present invention have better moisturizing effects.

Test example five, scar removal effect test

1. test subjects: 180 volunteers aged 20-50 years, all of which had scars.

2. Test materials: handmade soaps prepared according to examples 1-3 of the present invention and comparative examples 1-3.

3. the test method comprises the following steps:

The volunteers were randomly divided into 6 groups, each of which was washed twice in the morning and in the evening with the handmade soap prepared in examples 1-3 and comparative examples 1-3, respectively, and used continuously for two months. The scar-removing effect is evaluated by observing the red and swollen condition of the scar with naked eyes and consulting the improvement condition of the itching feeling of the volunteers.

4. And (3) test results: the test results are shown in table 6.

TABLE 6 scar-removing Effect test results

Categories	Effective (human)	slightly effective (human)	Invalid (human)	total effective rate (%)
					Example 1	20	7	3	90
example 2	24	4	2	93
					Example 3	21	6	3	90
Comparative example 1	13	10	7	77
					Comparative example 2	10	11	9	70
comparative example 3	7	12	11	63

As can be seen from Table 6, the handmade soap prepared by the invention has a good scar removing effect, and does not cause adverse reactions such as allergic phenomena of subjects. The effect of the example 2 is best, the total effective rate reaches 93%, and the total effective rate of removing scars of the comparative examples 1-3 is only 77%, 70% and 63%, so that the skin moisturizer formed by compounding squalane, chitosan oligosaccharide and argy wormwood leaf essential oil in the handmade soap has a better scar removing effect.

Test example six, stability test

1. Test materials: handmade soaps prepared according to examples 1-3 of the present invention and comparative examples 1-4.

2. The test method comprises the following steps:

After the soap is wrapped by a preservative film, the soap is placed indoors for ten months at normal temperature, and the change of the soap body is observed in the period.

3. and (3) test results: the test results are shown in table 7.

Table 7 stability test results

as can be seen from Table 7, the handmade soaps prepared in examples 1-3 of the present invention did not change color after being left for ten months at room temperature, while the handmade soaps prepared in comparative examples 1-4 changed color to different degrees, indicating that the handmade soaps prepared in the present invention have better stability.

While the invention has been described in further detail with reference to specific preferred embodiments thereof, it should be understood that the invention is not limited to the specific embodiments thereof. For those skilled in the art to which the invention pertains, several simple deductions or substitutions may be made without departing from the spirit of the invention, and these deductions or substitutions should be considered as the protection scope of the invention.

Claims (4)

1. the handmade soap is characterized by being prepared from the following raw materials in parts by weight:

6-9 parts of sodium hydroxide, 20-40 parts of grease, 0.5-2 parts of citronella essential oil, 4-6 parts of emollient, 5-8 parts of ant extract and 30-42 parts of deionized water;

The grease consists of coconut oil, shea butter and cocoa butter according to the weight ratio of 1 (1-5) to 4-8;

The emollient is composed of squalane, chitosan oligosaccharide and folium artemisiae argyi essential oil according to the weight ratio of 1 (2-4) to (3-6);

the preparation method of the ant extract comprises the following steps:

(1) drying red ants, grinding in a ball mill, and sieving to obtain ant powder;

(2) Adding the ant powder obtained in the step (1) into a sodium bicarbonate solution with the mass concentration of 3-5%, stirring for 20-30min, filtering, and drying to obtain pre-treated ant powder;

(3) Adding rice wine 5-8 times the weight of the pretreated ant powder obtained in step (2), performing ultrasonic extraction at 40-52 deg.C for 40-90min, filtering, concentrating, and drying to obtain the final product;

In the step (2), the adding proportion of the ant powder to the sodium bicarbonate solution is 1 g: 5-10 mL;

The alcohol content of the rice wine in the step (3) is 15-20 degrees.

2. the handmade soap of claim 1, which is prepared from the following raw materials in parts by weight: 8 parts of sodium hydroxide, 30 parts of grease, 1.5 parts of citronella essential oil, 5 parts of emollient, 7 parts of ant extract and 36 parts of deionized water.

3. The handmade soap of claim 1, wherein the emollient is comprised of squalane, chitosan oligosaccharide and argyi leaf essential oil in a weight ratio of 1:3: 5.

4. A method of making handmade soap according to any of claims 1 to 3, comprising the steps of:

S1, dissolving sodium hydroxide in deionized water, and cooling to 40-50 ℃ to obtain alkali liquor;

s2, uniformly mixing the grease, and heating to 45-53 ℃ to obtain mixed grease;

S3, slowly adding the alkali liquor obtained in the step S1 into the mixed oil obtained in the step S2, stirring while adding, obtaining a viscous soap solution after 1-2 hours, then adding the citronella essential oil, the emollient and the ant extract into the soap solution, stirring uniformly, pouring into a mold, standing at normal temperature for 24-40 hours, and demolding to obtain a molded solid;

s4, placing the molded solid obtained in the step S3 in a cool and ventilated place for curing for 30 days, and obtaining the product.