CN107926779A - A kind of Tachypleus tridentatus suspension artificial incubation method - Google Patents
A kind of Tachypleus tridentatus suspension artificial incubation method Download PDFInfo
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- CN107926779A CN107926779A CN201711102233.2A CN201711102233A CN107926779A CN 107926779 A CN107926779 A CN 107926779A CN 201711102233 A CN201711102233 A CN 201711102233A CN 107926779 A CN107926779 A CN 107926779A
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- Prior art keywords
- basket
- hatching
- horseshoe crab
- ovum
- water
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of Tachypleus tridentatus suspension artificial incubation method.The invention mainly includes steps：The first, hatchery is set；2nd, hatching basket is prepared；3rd, selection and disinfection horseshoe crab ovum；4th, place horseshoe crab ovum and enter basket；5th, daily management.The hatching basket is up-narrow and down-wide conical plastic basket, and there is hole at basket wall and basket bottom.Basket mouth is provided with light material foam.Horseshoe crab ovum is placed in the hatching basket to suspend in water by the present invention hatches, environment residing for horseshoe crab ovum has substantial amounts of oxygen and water, compared to the hatching method being placed in horseshoe crab ovum in sand, eliminate the disinfection to sand and water and do dew cleaning process, avoid the transfer operation in hatching process.Hatching basket of the present invention is up-narrow and down-wide, and basket mouth is smaller, and basket limits horseshoe crab ovum and taken out of by substantial amounts of water outside basket in cone, inclined basket wall.Pass through above-mentioned improved hatching method, it is possible to increase incubation rate, shortens brooding time, avoid horseshoe crab ovum from dropping out outside incubation vessel.
Description
Technical field
The present invention relates to the artificial breeding technology of Tachypleus tridentatus, and in particular to a kind of Tachypleus tridentatus suspension artificial incubation method.
Background technology
In the horseshoe crab existing latter stage devonian period in the Paleozoic Era before 400,000,000 years, be a small number of ancient species only deposited on the earth.Tachypleus tridentatus
(Tachypleus tridentatus) is subordinate to the Merostomata of species rareness, also known as east horseshoe crab, three thorn horseshoe crabs, horse crab, Chinese horseshoe crab.
Tachypleus tridentatus appearance is in blue or green brown or crineous, is coated with hard crust, body is made of cephalothorax, belly and sword tail three parts.In
State horseshoe crab is in biological evolution, ocean is bionical, medicine, food etc. all have very high researching value.Amoeba in limulus blood
Cell extract is exactly usually described reagents, and examination is used as by the use of the amebocyte lysate (LAL) in the blue blood of horseshoe crab
Agent, can Sensitive Detection trace of bacteria endotoxin（LPS）, whether inside of human body tissue can be accurately and rapidly detected because of bacterium sense
Contaminate and cause a disease, in pharmacy and food industry, can be polluted and be monitored with its contratoxin.Demand of the existing market to Tachypleus tridentatus
Very big, the investigation and utilization of horseshoe crab resource are all attached great importance in countries in the world.The maturity period of horseshoe crab is up to 13 years, it is ripe after
Lay eggs on the sandy beach of coastal waters intertidal zone, hatch therewith.The artificial cultivating method of traditional Chinese horseshoe crab is that sand is laid with aquaculture pond
Nature environment is simulated, keeps water quality and sand quality, allows horseshoe crab natural spawning in sand to hatch.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of Tachypleus tridentatus suspension artificial incubation method, overcome current China
Horseshoe crab ovum artificial incubation operational sequence is numerous and diverse, and water quality keeps of high cost, and hatching efficiency is not high, horseshoe crab ovum easily holds from cultivation in breeding process
The shortcomings that being dropped out in device.
Foregoing invention technical problem is to employ following technical scheme, a kind of Tachypleus tridentatus suspension artificial incubation method, mainly
Comprise the following steps：
The first, hatchery is set：Horseshoe crab egg hatching pond specification is long 3.0~5.0m, wide 3.0~5.0m, high 1.0~1.5m, away from pond
Gap is set at 0.8~1.3m of bottom, and it is 0.8~1.3m to keep height of water level in hatchery；
2nd, hatching basket is prepared：Hatch the frame of plastic that basket is upper opening, air-blowing oxygenation head is placed in basket, along fixation on basket
There is light material, input hatchery is spare on the day of horseshoe crab ovum enters pond after hatching basket is sterilized；
3rd, selection and disinfection horseshoe crab ovum：Batch collection horseshoe crab ovum, the ovum of rejecting abnormalities adhesion, hardening and discoloration, uniformly carries out disinfection,
Washed with water after disinfection；
4th, place horseshoe crab ovum and enter basket：Horseshoe crab ovum is dipped with sieve spoon and is put into hatching basket, and it is 10~20/c ㎡ that horseshoe crab ovum, which places density,；
5th, daily management：Lotic hatching mode is taken, water 100% is being changed in streamflow control daily, keeps condition of water quality as hatching
20~33 DEG C of water temperature, salinity 20 ‰~35 ‰, pH value 7.4~8.5, by 45~69 days, horseshoe crab ovum can incubation of membrane.
The hatching basket is up-narrow and down-wide conical plastic basket, and there is hole at basket wall and basket bottom.
15~45cm of the hatching basket basket mouth diameter, basket bottom 20~50cm of diameter.
The mesh number of the hatching basket basket bottom outlet is 14 mesh.
The mesh number of the hatching basket basket cinclides is 12 mesh.
The light material is foam.
Traditional horseshoe crab egg hatching method to be sanded in aquaculture pond, simulation horseshoe crab coastal waters intertidal zone in nature lay eggs from
Right environment, ovum is hatched in sand after horseshoe crab oviposition, and horseshoe crab ovum is placed in sand by this hatching method, water quality and sandy easily change,
Often carry out disinfection to aquaculture pond, do dew, clean, complex procedures, high labor cost, and since aforesaid operations can be to horseshoe crab egg hatching
Impact, causing incubation rate, brooding time is slower less than 50%.The present invention sets hatching basket in aquaculture pond, hangs hatching basket
Floating expose the surface 2-3cm on basket top, neither contact chamber bottom in water, only to expose the surface more, places air-blowing in basket and fills
Oxygen head supplemental oxygen, horseshoe crab ovum is placed in basket and is hatched, and the existing water of the environment residing for horseshoe crab ovum has substantial amounts of oxygen again, compared to traditional
Hatch in sand, incubation rate higher.Because horseshoe crab ovum is placed in hatching basket, sand no longer is laid with aquaculture pond bottom, is eliminated a large amount of
Sterilize, the process of dry dew and cleaning, save manpower and materials, it also avoid the transfer to horseshoe crab ovum in the hatching process of horseshoe crab ovum
Operation.Hatching basket in the present invention is the conical plastic charging basket of up-narrow and down-wide shape, by barrel-shaped with diameter with square basket, upper and lower opening
Basket contrast test, because placing air-blowing oxygenation head in frame, can cause water in acutely boiling because being constantly filled with a large amount of oxygen in basket
Shape, using square basket or upper and lower opening with the barrel-shaped basket of diameter, basket mouth is larger or upper and lower diameter is identical, and the water that horseshoe crab ovum can be filled with is taken out of
Basket is outer, is unable to reach the effect for being placed in hatching in basket, hatching basket of the present invention is up-narrow and down-wide, and basket mouth is smaller, and basket is in
Cone, inclined basket wall limit horseshoe crab ovum and are taken out of by water.The mesh number of hatching basket basket bottom and basket cinclides is limited to 12-20 mesh, greatly
In 12 mesh, horseshoe crab ovum is easily dropped out or gone out from hole, is deteriorated less than 20 mesh, and extraneous water coke slurry effect.Pass through above-mentioned improved hatching
Method, it is possible to increase incubation rate, shortens brooding time.
Embodiment
Embodiment 1
The first, hatchery is set：Choose 3 mouthfuls of horseshoe crab ovum suspension hatcheries, a height of 5.0m*5.0m*1.2m of length and width, at away from bottom of pond 1.0m
Gap is set, ensures water level 1.0m in hatchery.
2nd, hatching basket is prepared：Choose the conical flat-bottomed plastics circle basket of basket mouth diameter 20cm, basket bottom diameter 40cm, basket wall and
There is a hole at basket bottom, and the mesh number of hatching basket basket cinclides is 12 mesh, 14 mesh of mesh number of basket bottom outlet.Along foam floating block is set on basket, keep
Basket upper oral part exposes the surface 2.5cm.Soaked 30 minutes and sterilized with 50ppm liquor potassic permanganates, ready hatching basket is through disappearing
Input hatchery is spare on the day of horseshoe crab ovum enters pond after poison, and every basket of placement air-blowing, which is oxygenated first, makes water in basket keep boiling-like.
3rd, place horseshoe crab ovum and enter basket：Horseshoe crab ovum 1.2kg is collected from Tachypleus tridentatus spawning pond, rejecting abnormalities adhesion, hardening and discoloration
Ovum, is soaked 15 minutes with 20ppm Compound New Nomin solution and carries out surface sterilization, and hatching basket is put into after careful cleaning.
4th, hatching density is controlled：Horseshoe crab ovum is dipped with stainless steel mesh spoon and is put into hatching basket hatching, and each basket of hatching places horseshoe crab
5024, ovum, density domination is in 4/c ㎡.
5th, daily management：Lotic hatching mode is taken during the suspension hatching of horseshoe crab ovum, the every water quality physics and chemistry of detection daily refers to
Mark.Keep inflation in hatching basket enough uniformly, in 20~30 DEG C of water temperature of hatching, salinity 25 ‰~33 ‰, pH value 7.6~8.5
Pass through the artificial culture horseshoe crab egg hatching membrane of 69 days, horseshoe crab egg hatching rate 93% under part.
Embodiment 2
The first, hatchery is set：Choose 5 mouthfuls of horseshoe crab ovum suspension hatcheries, a height of 3.0m*3.0m*1.0m of length and width, at according to bottom of pond 0.8m
Gap is set, ensures water level 0.8m in hatchery.
2nd, hatching basket is prepared：Choose the conical flat-bottomed plastics circle basket of basket mouth diameter 15cm, basket bottom diameter 20cm, basket wall and
There is a hole at basket bottom, and the mesh number of hatching basket basket cinclides is 20 mesh, 12 mesh of mesh number of basket bottom outlet.Along foam floating block is set on basket, keep
Basket upper oral part exposes the surface 3cm.Soaked 30 minutes and sterilized with 50ppm liquor potassic permanganates, ready hatching basket is sterilized
It is spare that hatchery is put on the day of horseshoe crab ovum enters pond afterwards, every basket of placement air-blowing, which is oxygenated first, makes water in basket keep boiling-like.
3rd, selection and disinfection horseshoe crab ovum：Horseshoe crab ovum 0.9kg, rejecting abnormalities adhesion, hardening and discoloration are collected from Tachypleus tridentatus spawning pond
Ovum, it is unified to carry out surface sterilization, be put into hatching frame after careful cleaning.
4th, hatching density is controlled：Horseshoe crab ovum is quantitatively dipped with sieve spoon and is put into hatching frame hatching, and each frame of hatching places horseshoe crab ovum
3768, density domination is in 3/c ㎡.
5th, daily management：Lotic hatching mode is taken during the suspension hatching of horseshoe crab ovum, the every water quality physics and chemistry of detection daily refers to
Mark.Keep suspend hatching frame in inflation it is enough uniformly, hatching 30~33 DEG C of water temperature, salinity 20 ‰~32 ‰, pH value 7.4~
Pass through the artificial culture horseshoe crab egg hatching membrane of 45 days, horseshoe crab egg hatching rate 96% under the conditions of 8.3.
Embodiment 3
The first, hatchery is set：Choose 6 mouthfuls of horseshoe crab ovum suspension hatcheries, specification 3.8m*5m*1.5m, away from setting spilling water at bottom of pond 1.3m
Mouthful, ensure water level 1.3m in hatchery.
2nd, hatching basket is prepared：Choose the conical flat-bottomed plastics circle basket of basket mouth diameter 45cm, basket bottom diameter 50cm, basket wall and
There is a hole at basket bottom, and the mesh number of hatching basket basket cinclides is 20 mesh, 20 mesh of mesh number of basket bottom outlet.Along foam floating block is set on basket, keep
Basket upper oral part exposes the surface 3cm.Soaked 30 minutes and sterilized with 50ppm liquor potassic permanganates, ready hatching basket is sterilized
It is spare that hatchery is put on the day of horseshoe crab ovum enters pond afterwards, every basket of placement air-blowing, which is oxygenated first, makes water in basket keep boiling-like.
3rd, selection and disinfection horseshoe crab ovum：Horseshoe crab ovum 9kg is collected from Tachypleus tridentatus spawning pond, rejecting abnormalities adhesion, hardening and discoloration
Ovum, it is unified to carry out surface sterilization, it is put into hatching frame after careful cleaning
4th, hatching density is controlled：Horseshoe crab ovum is quantitatively dipped with stainless steel mesh spoon and is put into hatching frame hatching, and each frame of hatching places horseshoe crab
39250, ovum, density domination is in 5/c ㎡.
5th, daily management
Lotic hatching mode is taken during the suspension hatching of horseshoe crab ovum, the every water quality physical and chemical index of detection daily.Keep the hatching frame that suspends
Interior inflation is enough uniformly, in 28~33 DEG C of water temperature of hatching, salinity 25 ‰~35 ‰, by 58 days under the conditions of pH value 7.4~8.5
Artificial culture horseshoe crab egg hatching membrane, horseshoe crab egg hatching rate 92%.
Claims (5)
1. a kind of Tachypleus tridentatus suspension artificial incubation method, it is characterised in that the method mainly includes the following steps that：
The first, hatchery is set：Horseshoe crab egg hatching pond specification is long 3.0~5.0m, wide 3.0~5.0m, high 1.0~1.5m, away from pond
Gap is set at the 0.8-1.3m of bottom, and it is 0.8~1.3m to keep height of water level in hatchery；
2nd, hatching basket is prepared：Hatch the plastic crate that basket is upper opening, air-blowing oxygenation head is placed in basket, along fixation on basket
There is light material, input hatchery is spare on the day of horseshoe crab ovum enters pond after hatching frame is sterilized；
3rd, selection and disinfection horseshoe crab ovum：Batch collection horseshoe crab ovum, the ovum of rejecting abnormalities adhesion, hardening and discoloration, the unified surface that carries out disappear
Poison, washes with water after disinfection；
4th, place horseshoe crab ovum and enter basket：Horseshoe crab ovum is dipped with sieve spoon and is put into hatching basket, and it is 3~5/c ㎡ that horseshoe crab ovum, which places density,；
5th, daily management：Lotic hatching mode is taken, water 100% is being changed in streamflow control daily, keeps condition of water quality as hatching
20~33 DEG C of water temperature, salinity 20 ‰~35 ‰, pH value 7.4~8.5, by 45~69 days, horseshoe crab ovum can incubation of membrane.
A kind of 2. hatching method as claimed in claim 1, it is characterised in that：The hatching basket shapes for up-narrow and down-wide circular cone
There is hole at charging basket, basket wall and basket bottom.
A kind of 3. hatching method as claimed in claim 2, it is characterised in that：15~45cm of the hatching basket basket mouth diameter, basket
Bottom 20~50cm of diameter, hatches a diameter of 12-20 mesh in the hole at basket basket wall and basket bottom.
A kind of 4. hatching method as claimed in claim 3, it is characterised in that：The mesh number of the hatching basket basket bottom outlet is 14 mesh.
A kind of 5. hatching method as described in claim 1-4, it is characterised in that：The light material is foam.
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| CN201711102233.2A CN107926779A (en) | 2017-11-10 | 2017-11-10 | A kind of Tachypleus tridentatus suspension artificial incubation method |
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| CN106719167A (en) * | 2016-12-12 | 2017-05-31 | 中国水产科学研究院东海水产研究所 | A kind of six spot pierces the incubation equipment and hatching method of Puffer embryonated egg |
| CN107182848A (en) * | 2016-12-28 | 2017-09-22 | 安徽农业大学 | A kind of Spinibarbus sinensis artificial fecundation method |
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2017
- 2017-11-10 CN CN201711102233.2A patent/CN107926779A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3008993A1 (en) * | 2013-06-15 | 2016-04-20 | Googol Holdings Co., Ltd | Method for inducing artificial ovulation and spawning of fresh-water eels |
| CN103621440A (en) * | 2013-11-18 | 2014-03-12 | 常州日美水产科技有限公司 | Incubation system and incubation method of adhesive roe |
| CN104170783A (en) * | 2014-09-05 | 2014-12-03 | 广西壮族自治区水产科学研究院 | Efficient breeding method suitable for limuli from germ cells to 2-age phase |
| CN104872067A (en) * | 2015-05-04 | 2015-09-02 | 钦州学院 | Method for increasing egg laying amount of egg-laying female tachypleus tridutatus |
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Application publication date: 20180420 |