CN104872067A - Method for increasing egg laying amount of egg-laying female tachypleus tridutatus - Google Patents
Method for increasing egg laying amount of egg-laying female tachypleus tridutatus Download PDFInfo
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- CN104872067A CN104872067A CN201510220322.1A CN201510220322A CN104872067A CN 104872067 A CN104872067 A CN 104872067A CN 201510220322 A CN201510220322 A CN 201510220322A CN 104872067 A CN104872067 A CN 104872067A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention relates to a method for increasing egg laying amount of egg-laying female tachypleus tridutatus. The method includes that tachypleus tridutatus egg laying pools are arranged to be 2mx2mx(1-4)m, parent tachypleus tridutatus which are bought are placed into the egg laying pools at two ends in a male-female pairing manner, depth of water in each pool is 70-80cm, constant convection is performed, air stone is placed in each pool for inflation, and water which is used is fresh seawater after sand filtering and sedimentation; each other day, fresh oyster 150-200g is fed to each pair of parent tachypleus tridutatus at 10pm, water is changed in the morning of the next day, sand is laid at the bottom of each pool, water temperature is 27.5-31 DEG C, pH value is 7.8-8.0, dissolved oxygen is 6.3-7.0, illuminance is 60-3000lx, and salinity is 3.2-3.5%. Female tachypleus tridutatus of 6-8.5kg is selected for breeding, and egg laying amount can reach more than 14000. By correctly selecting female tachypleus tridutatus with proper weight, breeding cost can be lowered, and egg laying efficiency can be improved.
Description
Technical field
The invention belongs to technical field of aquaculture, relate to a kind of raising and to lay eggs the method for female Tachypleus tridentatus egg laying amount, particularly relate to a kind of method being improved female Tachypleus tridentatus egg laying amount of laying eggs by body weight screening.
Background technology
Tachypleus tridentatus (Tachypleus tridentatus), be subordinate to Arthropoda (Arthropoda), have huge legendary turtle limb subphylum (Chelieerta), Merostomata (Merostomata), Xiphosura (Xiphosura), king crab section (Limulidae).In the embryo of king crab and early stage larval development, have the trilobita stage, it is close relative that it and early Cambrian period (before about 5.7 hundred million years) occur to the trilobita that the late Permian Period (before 2.5 hundred million years) dies out.Present king crab is the animal that Merostomata is only deposited, and Merostomata starts from the Cambrian, is tending towards flouring when the Silurian Period, the devonian period (4.35-3.6 is before 100000000 years), starts decline Mesozoic Era (before about 2.4 hundred million years), only deposits king crab so far.Class king crab (Merostomata xiphosuran) fossil the earliest sees the late Ordovician period (before about 4.5 hundred million years), and the form of king crab is at that time just very similar to modern king crab.Existing king crab has experienced the very long course of about several hundred million years, and morphosis does not have large change, so king crab is genuine " living fossil ".
King crab is the important materials of research organic evolution, animal physiological and bionic, so it is a member indispensable in the Nature, is also the important component part of ecological chain.King crab plays an important role in the scientific research fields such as zoology, organic evolution, ecology, modern medicine and bionics, for the mankind have made outstanding contributions, is precious, irreplaceable natural resources.In the history of evolution of the earth, once there is more than 100 kind of king crab.But now, the earth only deposits four kinds: a kind of limulus polyphemus is distributed in East Coast of North America; Other three kinds of king crabs are distributed in east Asia and Southeast Asia one with narrow marine site.King crab is a kind of precious marine medicinal animals resource, can not only TAL processed, also can be separated the antibacterial peptide of AntiHIV1 RT activity, fungi, Gram-negative bacteria from king crab.
Tachypleus tridentatus is individual the maximum in present four kinds of king crabs, and its meat and ovum can eat, and its meat, shell and tail are the medicines controlling traumatic injury, high heat, can also chitin extraction.In view of this Tachypleus tridentatus has important economy and researching value.In recent years, the population quantity of Tachypleus tridentatus is fewer and feweri, has now taken turns as animals on the brink of extinction.Do be the sharply minimizing of Tachypleus tridentatus quantity that human behavior causes, or whole world change causes? people also have a lot of arguement.After all, why Tachypleus tridentatus is only distributed in north to north, Japanese Kyushu Island bank, reaches the west and south, Indonesia Su Mendala island in the south, to the east of Philippine, is still a fan in the narrow marine site of South East Asia Mainland concerning the mankind.
Tachypleus tridentatus individuality is comparatively large, general more than 4.4 jin, a traditional unit of weight maturations.From 4.4 jin, a traditional unit of weight to 6 jin, a traditional unit of weight, egg laying amount increases gradually.Choosing ripe Tachypleus tridentatus is close king crab, and this is the prerequisite of laying eggs.Tachypleus tridentatus is individual the maximum in present four kinds of king crabs, and female king crab body weight can reach more than 10 jin, a traditional unit of weight.In general, the female king crab of Tachypleus tridentatus is more ripe, body weight is heavier, and egg laying amount is larger; But Tachypleus tridentatus body weight is heavier, and price is higher.In prior art, lack a kind of by selecting the Tachypleus tridentatus of Suitable Body Weight, to improve the method for Tachypleus tridentatus egg laying amount.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of method being improved female Tachypleus tridentatus egg laying amount of laying eggs by body weight screening.
Raising is laid eggs the method for female Tachypleus tridentatus egg laying amount, adopts following technical scheme:
(1) the female king crab of Tachypleus tridentatus choosing 3.0-4.25kg is cooked close king crab and carries out nursery;
(2) close king crab male and female are put into spawning pond in pairs, spawning pond is between 2m × 2m × (1-4) m, pond depth of water 70-80cm, carry out constant convection current, with water be through sand filter after reppd fresh seawater, water temperature 27.5-31 DEG C, pH value 7.8-8.0, dissolved oxygen 6.3-7.0, illumination 60-3000lx, salinity 32-35 ‰, put into the inflation of gas stone in pond, at the bottom of pond, paving is husky;
(3) every other day, often pair of close king crab is thrown something and fed fresh oyster 150-200g, changes water.
Further preferably, at the bottom of described pond, paving is husky, and sand bed thick 15-20cm, Sha Jing are 0.4-2mm.
Further preferably, evening, 10:00 threw something and fed, and the next morning, 8:00 changed water.
Compared with prior art, beneficial effect of the present invention:
The present invention screens by body weight the method that female Tachypleus tridentatus of laying eggs improves egg laying amount, the female king crab of Tachypleus tridentatus choosing 3.0-4.25kg carries out nursery, and its egg laying amount can reach more than 14000, correctly chooses the female king crab of Suitable Body Weight, both can reduce seedling cost, can spawning efficiency be improved again.
Accompanying drawing explanation
Fig. 1 is 7 to the weight of female king crab and the linear relation of egg laying amount.
Fig. 2 is the linear relation between the weight of king crab between 5 jin to 8 jin and egg laying amount.
Embodiment:
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment:
Material
Parent king crab: experiment with Tachypleus tridentatus parent king crab, purchased from Zhanjiang Naozhou Island of Zhanjiang.Parent king crab length and width, heavily in table 1
Table 1 seven is to length and width, the weight of close king crab
Tab.2-1 The length，width and mass of 7pairs of female and male of Tachypleus tridentatus
The bait of parent king crab and the hatching young:
The bait of parent king crab is oyster; The bait of the young is prior cultured chlorella, flat algae, wheel animalcule, and benthic diatom, chrysophyceae, small animal and organic debris etc.
Instrument
Experiment salinometer (Japanese ATAGO, S-28E), dissolved oxygen meter (U.S. YSI, Pro-ODO), pH meter (Italian HANNA, HI8424), illumination meter (Taiwan ROC, TES1330A) is (education department) key lab of Guangdong Province---South Sea economic animal aquaculture laboratory equipment.
Experimental site equipment
Between seedling culturing vehicle, size is 32m × 24m × 2.7m.King crab spawning pond totally 4, pond size is 8m × 2m × 1m.2, king crab embryo breeding pond, size is consistent with spawning pond, is used for fertilized egg or embryo breeding to become young king crab.Support inside king crab embryo breeding pond and have Holothuria atra, in order to purify water, king crab embryo breeding basket floats on water surface.There is the glass sunroof 30 of 1.8m × 1.5m on top, workshop, and intensity of illumination fine day is 3780lx, and the cloudy day is 160lx.Special seawater, fresh water supply and charger is provided with in workshop.
Method
Parent king crab is supported temporarily and lays eggs:
King crab spawning pond is separated into four parts by plastic deeping, every part size 2m × 2m × 1m.The close king crab male and female bought paired (♀ ♂) are put into spawning pond.Pond depth of water 70-80cm, carries out constant convection current, puts into the inflation of gas stone in each little Chi.Reppd fresh seawater after twice husky filter with water.Every other day, often pair of close king crab is thrown something and fed fresh oyster 150-200g.10:00 bait throwing in evening, the next morning, 8:00 changed water.1-4, to paving afternoon on the 17th husky (the thick 15-20cm of sand bed, Sha Jing are 0.4-2mm), is at paving sand afternoon on the 18th in the 5th pair of pond.6th, 7 to being newly buy July 17 and 18 days, supporting temporarily, putting into the spawning pond that paving is husky on August 19 in not spreading in husky pond.Water temperature 27.5-31 DEG C, pH value 7.8-8.0, dissolved oxygen 6.3-7.0, illumination 60-3000lx, salinity 32-35 ‰.After parent king crab lays eggs, individual hour of 6-10, pulls out fertilized egg after cleaning, and puts into the hatching of king crab embryo breeding pond.
Data processing method
Embryonic development is to the time in each period, and body weight and diameter Relationship adopt Excel 2003 to process.Carry out the one-way analysis of variance (One-Way ANOVA) of other data with SPSS17.0, carry out Multiple range test with Duncan, if p < 0.05 is significant difference.
Result:
The situation of laying eggs of parent king crab:
It is right that parent king crab is all formed before laying eggs, and namely male king crab keeps a firm hand on both sides, female king crab plastron rear end with half chela of the 2nd, 3 pair of thorax appendage.Female king crab creeps forward exploration environment, when environment is suitable, just burrows with the 6th pair of thorax appendage and lays eggs.Ovum is faint yellow spherical in shape, ovum footpath 3-3.4mm.After female king crab lays eggs, sperm is just sprinkled upon on ovum and makes it be fertilized by male king crab.Successively to lay eggs test to close king crab with 7, situation of laying eggs in detail is in table 2.
The situation of laying eggs of table 2 seven pairs of Tachypleus tridentatus parent king crabs
Tab.2-2 The Spawning situation of 7pairs of female and male Tachypleus tridentatus
From table 2, front 5 pairs of Tachypleus tridentatus number of times of laying eggs is more, general 5-6 time (average 5 times), and general more than 11000 of single king crab egg laying amount, single king crab egg laying amount can reach 16310 at most.Rear 2 pairs of Tachypleus tridentatus lay eggs number of times reduce (3-5 time, average 4 times), egg laying amount minimizing, general less than 7954.Maximum single egg laying amount can reach 7320.The often pair of king crab eggs on average 4.71 times, average single egg laying amount 2370.92/time, on average single king crab egg laying amount 11184.29; Average shell rate 92%; Average percentage hatch rate 90%.This nursery, 78290,7 king crab common property ovum, bring out Tachypleus tridentatus seedling 70461.
Tachypleus tridentatus egg laying amount and dam king crab weight relation:
The weight of 7 pairs of female king crabs and the linear relation of egg laying amount are shown in Fig. 1.
7 is y=2582.7x-7868.9, R to the linear equation between the weight of female king crab and their egg laying amounts as seen from Figure 1
2=0.5828.R
2be worth on the low side, illustrate that linear relation is not obvious.
If by more than 8 jin 3 female king crab removings, the linear relation between the weight of the king crab between only analyzing 5 jin to 8 jin and their egg laying amounts, result is as Fig. 2.
As seen from Figure 2, the linear equation between the weight of 4 female king crabs and egg laying amount is y=4968.3x-22009, R
2=0.984.R
2be worth high, close to 1, linear relation clearly.
This illustrates, linear between the female king crab weight of 5-8 jin and egg laying amount, and between the weight of female king crab more than 8 jin and egg laying amount, linear relation is not obvious.
The relation of female king crab egg laying amount king crab body weight female with it
As seen from Figure 2, linear between the female king crab weight of 5-8 jin and egg laying amount, and between the weight of female king crab more than 8 jin and egg laying amount, linear relation is not obvious, this may be older with the female king crab more than 8 jin, belongs to old king crab, can affect egg laying amount.
King crab from development of fertilized ova to adult about 14 years, so just current, carry out artificial feeding and is worth little.But king crab " living fossil " animal that to be a kind of preciousness rare, its economic worth is increasing, and quantity of the catch is also larger, and its growth cycle is long, and the quantity of king crab sharply reduces.In order to protect this distinctive precious aquatic/marine animals resource, a large amount of artificial breeding from now on, raise after 2 years when it has an adaptive capacity to natural enemy, put and return sea, supplementary marine resources, should be the method for an effective maintenance Tachypleus tridentatus resource.Meanwhile, artificial breeding, also for study its grow and various biological phenomena material is provided.
The above, be only best mode for carrying out the invention, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses, and the simple change of the technical scheme that can obtain apparently or equivalence are replaced and all fallen within the scope of protection of the present invention.
Claims (3)
1. raising is laid eggs a method for female Tachypleus tridentatus egg laying amount, it is characterized in that: adopt following technical scheme:
(1) the female king crab of Tachypleus tridentatus choosing 3.0-4.25kg is cooked close king crab and carries out nursery;
(2) close king crab male and female are put into spawning pond in pairs, spawning pond is between 2m × 2m × (1-4) m, pond depth of water 70-80cm, carry out constant convection current, with water be through sand filter after reppd fresh seawater, water temperature 27.5-31 DEG C, pH value 7.8-8.0, dissolved oxygen 6.3-7.0, illumination 60-3000lx, salinity 32-35 ‰, put into the inflation of gas stone in pond, at the bottom of pond, paving is husky;
(3) every other day, often pair of close king crab is thrown something and fed fresh oyster 150-200g.
2. a kind of raising according to claim 1 is laid eggs the method for female Tachypleus tridentatus egg laying amount, it is characterized in that: at the bottom of described pond, paving is husky, and sand bed thick 15-20cm, Sha Jing are 0.4-2mm.
3. a kind of raising according to claim 1 is laid eggs the method for female Tachypleus tridentatus egg laying amount, and it is characterized in that: evening, 10:00 threw something and fed, the next morning, 8:00 changed water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510220322.1A CN104872067A (en) | 2015-05-04 | 2015-05-04 | Method for increasing egg laying amount of egg-laying female tachypleus tridutatus |
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| CN201510220322.1A CN104872067A (en) | 2015-05-04 | 2015-05-04 | Method for increasing egg laying amount of egg-laying female tachypleus tridutatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926779A (en) * | 2017-11-10 | 2018-04-20 | 广西壮族自治区海洋研究所 | A kind of Tachypleus tridentatus suspension artificial incubation method |
| CN110036954B (en) * | 2019-05-29 | 2021-03-09 | 上海海洋大学 | Method for accelerating sexual maturation of adult horseshoe crabs of Tachypleus tridentatus and culture system using same |
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| WO2000001400A1 (en) * | 1998-07-02 | 2000-01-13 | Associates Of Cape Cod, Inc. | Compositions comprising endotoxin neutralizing protein and derivatives and uses thereof |
| JP2004016234A (en) * | 2003-05-14 | 2004-01-22 | Tamao Noguchi | Method for cultivating globefish and method for detoxifying globefish using the method |
| CN1644043A (en) * | 2005-01-28 | 2005-07-27 | 洪水根 | Culture of king crab |
| CN102487853A (en) * | 2011-11-18 | 2012-06-13 | 洪水根 | Artificial breeding and culturing method for horseshoe crabs |
-
2015
- 2015-05-04 CN CN201510220322.1A patent/CN104872067A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000001400A1 (en) * | 1998-07-02 | 2000-01-13 | Associates Of Cape Cod, Inc. | Compositions comprising endotoxin neutralizing protein and derivatives and uses thereof |
| JP2004016234A (en) * | 2003-05-14 | 2004-01-22 | Tamao Noguchi | Method for cultivating globefish and method for detoxifying globefish using the method |
| CN1644043A (en) * | 2005-01-28 | 2005-07-27 | 洪水根 | Culture of king crab |
| CN102487853A (en) * | 2011-11-18 | 2012-06-13 | 洪水根 | Artificial breeding and culturing method for horseshoe crabs |
Non-Patent Citations (1)
| Title |
|---|
| 谢叶: "中国鲎工厂化育苗及关键生态与鲎河豚毒素研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926779A (en) * | 2017-11-10 | 2018-04-20 | 广西壮族自治区海洋研究所 | A kind of Tachypleus tridentatus suspension artificial incubation method |
| CN110036954B (en) * | 2019-05-29 | 2021-03-09 | 上海海洋大学 | Method for accelerating sexual maturation of adult horseshoe crabs of Tachypleus tridentatus and culture system using same |
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Application publication date: 20150902 |