CN107916222A - The accurate packing of a kind of DNA primer or probe and mixing arrangement and its application method - Google Patents
The accurate packing of a kind of DNA primer or probe and mixing arrangement and its application method Download PDFInfo
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- CN107916222A CN107916222A CN201711270989.8A CN201711270989A CN107916222A CN 107916222 A CN107916222 A CN 107916222A CN 201711270989 A CN201711270989 A CN 201711270989A CN 107916222 A CN107916222 A CN 107916222A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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Abstract
The invention discloses a kind of DNA primer or the accurate packing of probe and mixing arrangement and its application method, this is precisely dispensed and mixing arrangement is mainly made of switch board, executing agency, working region component, test cell, pipette tips frame, working plate and stationary platen；Executing agency is made of X-axis slide unit, Y-axis slide unit, Z axis slide unit and rifle axis slide unit；Working region component includes 8 pieces of packing Target Boards, 1 block of pipette tips plate, 1 block of stoste plate, 1 piece of drain Target Board and 1 pipette tips separator, working plate and uses the distribution form centered on stoste plate.The present invention provides a kind of accurate, reliable, efficient engineered solution, is precisely dispensed so as to fulfill the automation of DNA primer or probe with mixing.The present invention can save and manually, can save cumbersome manual imbibition and beat liquid process, and operator only needs that required operation is imported software using mobile USB flash disk, and the preparation before eliminating, realizes full-automatic operation.
Description
Technical field
The invention belongs to technical field of molecular biology, particularly relates to precisely dividing for a kind of DNA primer or probe
Dress and mixing arrangement and its application method.
Background technology
In molecular biosciences research, according to specific purpose, it is necessary to synthesize the primer of different bases sequence and length.DNA draws
Thing in post synthesis, is generally in the orifice plate of standard, such as 48 orifice plates or 96 orifice plates, can be one different in each hole
Primer.Purpose according to analysis and research is different, it is sometimes desirable to will be synthetic same（Referred to as one）Primer or probe,
It is dispensed into several different centrifuge tubes or screwed pipe, needs different primer or probe being mixed into same pipe again sometimes
In son, the usually work of these packing or mixing is had been manually done by operating personnel by liquid-transfering gun.Complete different primers in 96 holes
The packing of unequal number amount, or different primers are mixed according to different quantity, an inherently very complicated work
Make.Since it is desired that packing or mixing primer or probe with microlitre measure, it is very high to the skill set requirements of operating personnel, be often difficult to
By measurement deviation or undulated control in smaller range.Meanwhile manual operations can neither avoid human error completely, but it is poor in appearance
Stagger the time is difficult to be found.
Laboratory liquor removing workstation or instrument for extracting nucleic acid of the prior art, are only capable of realizing certain liquid from a kind of container
Amount is transferred to the purpose of another container, can not realize primer or probe packing, the complicated and accurate requirement of mixing.It is counted
Amount unit is usually milliliter magnitude, and measuring accuracy is 1% or so, and the packing or mixing of primer or probe, measurement unit are micro-
Rising amount level, measuring accuracy require higher, and laboratory liquor removing workstation or instrument for extracting nucleic acid can not meet the height of this micro-measurement
Precision and high-stability requirement.And laboratory liquor removing workstation or instrument for extracting nucleic acid can not carry out realizing the automatic of data module
Collection, can not realize the compatible and connection with DNA primer or probe synthesis system.
The content of the invention
The present invention is in order to overcome the shortcomings of the prior art, it is contemplated that realizing the dress dispensed with mixing two big functions
Put and method, including realize the computer system control of these functions, there is provided the accurate packing of a kind of DNA primer or probe and mixed
Attach together and put and its application method.
The present invention is achieved by the following technical solutions：The accurate packing of a kind of DNA primer or probe and mixing arrangement,
It is mainly made of switch board, executing agency, working region component, test cell, pipette tips frame, working plate and stationary platen；
Executing agency is made of X-axis slide unit, Y-axis slide unit, Z axis slide unit and rifle axis slide unit；Fixed in the negative direction upper surface of stationary platen
Switch board is connected with, X-axis slide unit is fixed at the positive direction upper surface of stationary platen and is fixedly connected with switch board；Working
Working region component is fixedly connected with platen, working region component includes 8 pieces of packing Target Boards, 1 block of pipette tips plate, 1 piece of stoste
Plate, 1 piece of drain Target Board and 1 pipette tips separator, working plate use the distribution form centered on stoste plate, 8 pieces of packing
Target Board is uniformly distributed in the both sides of stoste plate X-direction, 4 pieces of packing Target Boards of positive direction, and 4 pieces of negative direction dispenses Target Boards,
Pipette tips frame is arranged in the Y-axis positive direction of stoste plate, and the Y-axis that drain Target Board and pipette tips separator are respectively arranged in stoste plate is born
On direction.
X-axis slide unit is connected with Y-axis slide unit using planer type structure, and the upper surface of X-axis slide unit is fixedly installed X-axis planker,
It is bolted two root posts on X-axis planker to hold up Y-axis slide unit, Z axis slide unit uses the structure of side lifting with Y-axis slide unit
Form connects, and is fixedly installed Y-axis planker in the upper surface of Y-axis slide unit, Z axis slide unit, rifle axis are fixedly installed on Y-axis planker
Slide unit is arranged on the side of Z axis slide unit.
Internal controller, DC power supply, driver and dress centrally-mounted driving are provided with switch board, fills centrally-mounted driving
Drive X-axis slide plate, Y-axis slide plate, Z axis slide plate and rifle axis skateboarding.The surface of packing target version is provided with 12 × 5 holes position,
The half elliptic step protrusion for fixing centrifuge tube tube cover is provided with the upper right corner of each hole position.Test cell is arranged on
The upper surface of working plate is simultaneously located in the Y-axis positive direction of stoste plate.
The invention also discloses a kind of DNA primer or the accurate packing of probe and the application method of mixing arrangement, including point
Dress or mixing demand parameter obtain step, Extraction medium step and liquid relief and quantitative into specified pipe or plate from specified pipe or plate
Medium step totally three steps are got, the specific operation process of each step is：
Step 1: packing or the acquisition of mixing demand parameter：
1）In the selection of computer control interface and DNA synthesizer on-line operation or off-line operation；
2）Select packing pattern or mixed mode；
3）Read demand data, that is, the specific requirement for dispensing or mixing；
When precisely packing and mixing arrangement are with DNA synthesizer on-line operation, in DNA synthesizes digital management system, according to visitor
The demand input of family order, device realize the direct of demand data using digital module collection compatible and management system
Import；
When precisely packing and mixing arrangement are with DNA synthesizer off-line operation, directly operating file is treated in the control computer of device
Press from both sides and establish packing or mixing demand information, including intend position, volume and the position that will be transferred to dispensed or blending agent is initial
Confidence ceases；
4）The combined operation that the demand parameter for dispensing or mixing is converted to each mechanism of device instructs；
Step 2: the Extraction medium from specified pipe or plate：
1）Host head unit links with the slipway mechanism of X-direction and Y-direction, is moved to above pipette tips frame；
2）Moved downward by host header Z axis, host header is fixed a liquid transfer gun head, then rise to appropriate height；
3）The host head unit of pipette tips has been plugged, has linked again with the slipway mechanism of X-direction and Y-direction, moves to stoste plate
Top, by moving downward for Z axis, make in the liquid in pipette tips insertion stoste plate corresponding hole site；
4）Motor driving piston rod on host header, by the DNA liquid extractions suction liquid transfer gun head of given dose；
5）Host header Z axis moves upwards, pipette tips is departed from stoste, and is lifted to appropriate height so that pipette tips in motion process
It will not be in contact with the plate in operating table surface or pipe；
Step 3: liquid relief and quantitatively getting medium into specified pipe or plate：
1）Linked by the slipway mechanism of X-direction and Y-direction, the host header for being loaded with DNA liquid is moved to corresponding centrifuge tube
Or above centrifugal hole；
2）Moved downward by host header Z axis, liquid transfer gun head is inserted into corresponding plate hole or centrifuge tube；
3）Motor driving piston rod on host header, corresponding plate hole or centrifuge tube are got by the DNA liquid push of given dose
It is interior；
4）Host header Z axis moves upwards, and is lifted to appropriate height, is ready for subsequent motion；
5）Host header links with the slipway mechanism of X-direction and Y-direction, is moved to pipette tips separator position, pipette tips separator blocks
Pipette tips, host header move upwards along Z axis, unload used pipette tips；
6）Host header moves to initial position, completes the packing or mixing liquid relief operation of a primer or probe.
According to the requirement for intending packing or mixing, there is the medium in several source plate holes to need to dispense or mix, with regard to repeat step
Two and step 3 several times.
In medicine and biology research and production, according to the purpose of certain medical treatment detection, diagnosis and research and development, there is difference
The primer or probe of base sequence and length are devised, and after the processes such as molecule synthesis, ammonolysis and purifying, finished product is deposited
It is stored in orifice plate or centrifuge tube.Then dispensed or mixed according to specific demand and needs again.
1st, dispense：
General dna primer finished product, is generally in the orifice plate of standard, such as 48 orifice plates or 96 orifice plates, is probably in each hole
One special primer of the different demands from different clients.Collect or keep according to the specific requirement of client, in each hole
Every primer is packed and is stored in centrifuge tube, it is necessary to be divided into the several pieces of definite dosage, is referred to as " plate is to pipe " and was dispensed
Journey.
DNA probe or some primer finished products by special purifying process, usually collect and are temporarily stored into larger centrifuge tube,
According to the specific requirement of client, it is necessary to be divided into the several pieces of definite dosage, pack and be stored in less centrifuge tube, be referred to as
Process is dispensed for " pipe is to pipe ".
The primer being temporarily stored into subsequently mix purpose in orifice plate or the primer being temporarily stored into larger centrifuge tube or spy
Pin is temporarily stored into the designation hole of 96 orifice plate of standard, it is necessary to be divided into several pieces according to certain dosage and share.Both were dispensed
Journey, the former is referred to as " plate is to plate " packing process, and the latter is referred to as " pipe is to plate " packing process.
2nd, mix：
Based on medicine and the purpose of biological study, it is sometimes desirable to by the different primers in 96 orifice plates or centrifuge tube or spy
Pin, is mixed according to certain dosage.It is similar with packing process, the process of mixing can also be " plate is to plate ", " pipe is to plate ",
The mixed process of " plate is to pipe " or " pipe is to pipe ".If in general, once need to complete the mixing compared with multiple types, and upon mixing again
Secondary packing continues to test, then final to mix more in 96 orifice plates；If only having a few, and no longer needed after mixing
Follow-up packing, then can directly mix into centrifuge tube.
DNA primer --- primer（primer）, also known as introduction.It is a bit of single stranded DNA or RNA, as rising for DNA replication dna
Initial point, in nucleic acid synthetic reaction, the polynucleotide chain that works as the starting point that each polynucleotide chain is extended,
On 3 '-OH of primer, nucleotide is synthesized in the form of diester chain, therefore 3 '-OH of primer must be free.
DNA probe --- gene probe, i.e. nucleic acid probe（probe）, it is one section and carries known to detection mark and order
, with the nucleotide sequence (DNA or RNA) of target gene complementation.
48 orifice plates or 96 orifice plates --- in scientific researches such as biologic medicals with being widely used in production field, as cell culture apparatus
Ware, quantitative fluorescent PCR platform consumptive material or nucleic acid collect temporary ware etc..
Centrifuge tube --- tubulose sample container, can with closure or gland, for storing synthetic primer or probe.
Accurate packing and mixing arrangement and the liquor removing workstation or instrument for extracting nucleic acid of medical laboratory in the present invention
It is more similar.With reference to the side such as primer or probe material performance characteristics in itself, packing or the particular/special requirement of mixing, control accuracy
Face, the present invention in apparatus and method provide a variety of innovative designing schemes, realize laboratory liquor removing workstation or
The function and solution that instrument for extracting nucleic acid can not be realized.
The beneficial effects of the invention are as follows：Precisely the purpose of packing and mixing arrangement is exactly to carry for DNA primer or probe of the present invention
For a kind of accurate, reliable, efficient engineered solution, including device is in itself and its application method, to realize DNA primer or spy
The automation of pin is precisely dispensed with mixing.Using the device and application method in the present invention, it can save manually, which can be with
Save cumbersome manual imbibition and beat liquid process, operator only needs that required operation is imported software i.e. using mobile USB flash disk
Can, the preparation before eliminating.The safety and stability that device completes work is significantly larger than artificial, will not be because of artificial
The reason for there is mistake.The precision of the present apparatus can reach within 2%, and can complete one piece in 30min or so and dispense Target Board
Operation is dispensed, realizes the packing and liquid relief work of high precision and large measuring range.The present apparatus can realize full-automatic operation, pass through dress
The integration put, the integration that the whole process in later stage can also be facilitated to automate.
The template that the present apparatus employs eight pieces of Target Boards of brand-new design is set, it is possible to achieve the big portion of DNA primer packing
The division of labor is required, and the operation such as plate is changed without midway pause.One-time pad can disposably complete the packing work of 96 primers, greatly
The big accuracy and speed for improving primer packing liquid relief, improves and purchases efficiency, ensure that the uniformity and reliability purchased.This
Invention can complete primer or probe from extracting to dispensing or the accurate measurement that mixes.Motion process is stable, reliable, positioning accurate
Really.Precisely the advantage of packing and mixing arrangement is the present invention：Slotting pipette tips, imbibition are realized, beat liquid and converts hole position and beats liquid again
Etc. function, the pipe of packing five is at best able to.
Brief description of the drawings
Fig. 1 is the structure diagram using apparatus of the present invention Extraction medium step from specified pipe/plate；
Fig. 2 is the dimensional structure diagram of present invention packing and mixing arrangement；
In Fig. 2：1- switch boards；2-X axis slide units；3-Y axis slide units；4-Z axis slide units；5- rifle axis slide units；6- test cells；7- is dispensed
Target Board；8- pipette tips framves；9- stoste plates；10- drain Target Boards；11- pipette tips separators；12- working plates；13- stationary platens；
14- holes position；15- half elliptics step is raised.
Fig. 3 is the control system hardware architecture diagram of present invention packing and mixing arrangement；
Fig. 4 is the connection mode and control principle schematic diagram of executing agency of the present invention；
Fig. 5 is the dimensional structure diagram that Target Board is dispensed in present invention packing and mixing arrangement；
Wherein：Switch board takes up all control sections, and test cell is used for realizing the test for beating liquid measure to device, dispenses target
Plate is used for taking up the primer after packing, and pipette tips frame is used for taking up liquid transfer gun head for pending trial, and stoste plate is used for taking up to be packed
Original primers.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
As shown in Figures 1 to 5, the accurate packing of a kind of DNA primer or probe and mixing arrangement, its mainly by switch board 1,
Executing agency, working region component, test cell 6, pipette tips frame 8, working plate 12 and stationary platen 13 form；Executing agency by
X-axis slide unit 2, Y-axis slide unit 3, Z axis slide unit 4 and rifle axis slide unit 5 form；It is fixedly connected in the negative direction upper surface of stationary platen 13
There is switch board 1, X-axis slide unit 2 is fixed at the positive direction upper surface of stationary platen 13 and is fixedly connected with switch board 1；In work
Make to be fixedly connected with working region component on platen 12, working region component include 8 blocks of packing Target Board 7,1 blocks of pipette tips plates, 1 piece
9,1 pieces of drain Target Boards 10 of stoste plate and 1 pipette tips separator 11, working plate 12 use the layout centered on stoste plate 9
Form, 8 pieces of packing Target Boards 7 are uniformly distributed in the both sides of stoste plate 9X direction of principal axis, 4 pieces of packing Target Boards 7 of positive direction, negative direction
4 pieces of packing Target Boards 7, pipette tips frame 8 are arranged in the Y-axis positive direction of stoste plate 9, and drain Target Board 10 and pipette tips separator 11 divide
It is not arranged in the Y-axis negative direction of stoste plate 9.This distribution form centered on stoste plate 9 can improve packing and drain
Speed, shorten packing and drain time.
X-axis slide unit 2 is connected with Y-axis slide unit 3 using planer type structure, and the upper surface of X-axis slide unit 2 is fixedly installed X-axis and drags
Plate, two root posts are bolted on X-axis planker and hold up Y-axis slide unit 3, and Z axis slide unit 4 is lifted with Y-axis slide unit 3 using side
Structure type connection, be fixedly installed Y-axis planker in the upper surface of Y-axis slide unit 3, be fixedly installed on Y-axis planker Z axis cunning
Platform 4, rifle axis slide unit 5 are arranged on the side of Z axis slide unit 4.Thereby realize the linear motion of three degree of freedom.
Internal controller, DC power supply, driver and dress centrally-mounted driving, device driving electricity are provided with switch board 1
Machine driving X-axis slide plate, Y-axis slide plate, Z axis slide plate and rifle axis skateboarding.The surface of packing target version is provided with 12 × 5 holes position
14, the upper right corner of each hole position 14 is provided with the half elliptic step protrusion 15 for fixing centrifuge tube tube cover.Test is single
Member 6 is arranged on the upper surface of working plate 12 and in the Y-axis positive direction of stoste plate 9.
The host header piston rod unit of apparatus of the present invention drives screw by a driving stepper motor by stepper motor
Convert rotational motion is the straight reciprocating motion of piston rod, to realize that drawing liquid beats the function of liquid.The helical pitch of screw is 2mm, piston
The straight reciprocating motion precision of bar is up to 0.1mm.Whole moving cell is made of three ball screw straight line slide units.Resetting
Precision can fully ensure that work reliable and stable under the high-precision state of device in below 0.02mm.
The structure of present invention packing and mixing arrangement is as shown in Figure 2.First, X-axis slide unit 2 uses planer-type with Y-axis slide unit 3
Structure type, the structure type can be greatly saved device space, facilitate upper liquid and take liquid to work without fixed upper beam.Z axis
Structure type of the slide unit 4 with Y-axis slide unit 3 using side lifting, facilitates the installation of Z axis slide unit 4 to safeguard.Rifle axis slide unit 5 is arranged on Z
On the side of axis slide unit 4, rifle axis slide unit 5 uses Open architecture, is convenient for changing the liquid getting device and its pipette tips of not co-content.Device
Using two layers of platen form, lower floor is the stationary platen 13 of installation X-axis slide unit 2, and upper strata is to place pipette tips frame 8, stoste plate 9, divide
Target Board 7 and the working plate 12 of the grade of drain Target Board 10 are filled, working plate 12 is fixed, and the liquid of required operation can be protected
Inactive state is held, ensure liquid picks and places quality.
The present invention packing and mixing arrangement control system hardware as shown in figure 3, control system hardware installation in device control
In cabinet 1 processed, mainly comprising device internal controller, DC power supply, driver and dress centrally-mounted driving.Device internal controller leads to
Cross RJ45 network interfaces to be attached with computer, receive packing drain data and instruction that computer transmission comes, controlled inside device
System is parsed packing drain data and instruction, and is converted to actuator voltage drive signal, is driven by driver
After signal amplification, X-axis slide plate, Y-axis slide plate, Z axis slide plate and rifle axis skateboarding are driven by filling centrally-mounted driving.Pass through pipette tips
Frame 8, stoste plate 9, the location data for dispensing Target Board 7 and drain Target Board 10, realize pipette tips frame 8, stoste plate 9, packing target
The target interpolation and movement of plate 7 and drain Target Board 10, driving pipette tips move to pipette tips frame 8, stoste plate 9,7 and of packing Target Board
The designated position of drain Target Board 10 carries out imbibition and tapping, so as to fulfill packing and the apparatus function of drain.
Executing agency is made of X-axis slide unit 2, Y-axis slide unit 3, Z axis slide unit 4 and rifle axis slide unit 5.The controlling party of each slide unit
Formula is identical, and is all independent control unit.The control principle of executing agency is with operation logic：
1）Operator makes packing drain data file first, and data are saved as excel data files；
2）Running gear operates software, will dispense drain data import operation software, and clear and definite specified device is packing Working mould
Formula or drain operating mode；
3）Controller inside computer and device is connected by RJ45 network connections, device operation software reads packing and leads automatically
Liquid data file；
4）Packing drain data conversion is instructed and sent by procotol to device internal control by device operation software in order to control
Device；
5）Control instruction is converted to X-axis, Y-axis, the moving interpolation voltage drive signals of Z axis by device internal controller, is sent extremely
Driver；
6）Driver will voltage signal amplification and distribution after, send to X-axis, Y-axis, Z axis driving motor；
7）X-axis, Y-axis, the motor driving pipette tips of Z axis move to pipette tips frame 8, stoste plate 9, packing Target Board 7 and drain Target Board
10 designated position；
8）The liquid for specifying liquid measure is drawn and placed to rifle spindle motor according to liquid measure order-driven rifle axis skateboarding；
9）After X-axis, Y-axis, the motor of Z axis and rifle axis receive halt instruction, stop motor operation, complete packing drain work.
Fig. 4 is the control principle drawing of slide unit：Motor is linked by shaft coupling and screw, and planker is fixed on the nut on screw
On.The rotary motion of oneself can be passed to planker by motor, it is finally completed straight reciprocating motion, the slide unit in each direction
Principle is identical.
As shown in Fig. 2, the present apparatus has been independently arranged calibrating position, a right hole is calibrating position pipette tips position, and interstitial hole is original
Fluid apertures, the first from left hole are calibration target hole.In Calibration interface drop-down menu, calibrating position can be corrected, operated herein
Person need to only calibrate pipette tips position.
As shown in figure 5, new design of the packing Target Board 7 using centrifuge tube frame plate, this centrifuge tube shelf are added by aluminum material
Work forms, and 12 × 5 holes position 14 is contained on surface, and 14 upper right corner of each hole position are provided with raised half elliptic step, for solid
Determine centrifuge tube tube cover.
No matter dispense or mixed process, the application method of apparatus of the present invention can be divided into packing/mixing demand parameter and obtain
Obtain, the Extraction medium from specified pipe or plate（Primer or probe）, liquid relief and several steps such as medium are quantitatively got into specified pipe/plate
Suddenly, the specific operation process of each step is：
Step 1：Packing or the acquisition of mixing demand parameter：
The purpose of this step is the data module of compatible universal, and the demand ginseng for needing to dispense or mixing is obtained in a manner of easily
Number, and be converted to the operation control instruction of device.
1.1st, in the selection of computer control interface and DNA synthesizer on-line operation or off-line operation；
1.2nd, packing pattern or mixed mode are selected；
1.3rd, demand data, that is, the specific requirement for dispensing or mixing are read；
When 1.3.1, with DNA synthesizer on-line operation, in DNA synthesis digital management systems, according to the demand of customer order
Through input, apparatus of the present invention are using digital module collection compatible and management system, it can be achieved that demand data is directly led
Enter；
When 1.3.2, with DNA synthesizer off-line operation, directly treat that operating file folder establishes packing in the control computer of the present apparatus
Or mixing demand information, including intend the position dispensed or blending agent is initial（Hole, pipe）, volume, the position that will be transferred to
（Hole, pipe）Etc. information；
The 1.4th, combined operation that the demand parameter for dispensing or mixing is converted to each mechanism of device instructs.
Step 2：The Extraction medium from specified pipe/plate：
The purpose of this step is according to the demand parameter for intending packing or mixing, real by the three-dimensional linkage of each motion
Liquid relief pipette tips are now fixed from specified plate hole or centrifuge tube, precisely extract the primer or probe of certain volume.
2.1st, the 1st step in Fig. 1：Host head unit links with the slipway mechanism of X-direction and Y-direction, is moved on pipette tips frame 8
Side；2nd step in Fig. 1：Moved downward by host header Z axis, host header is fixed a liquid transfer gun head, then rise to appropriate height
Degree；3rd step in Fig. 1：The host head unit of pipette tips has been plugged, has been linked again with the slipway mechanism of X-direction and Y-direction, movement
To the top of stoste plate 9 or pipe, by moving downward for Z axis, make the liquid in 9/ pipe corresponding hole site 14 of pipette tips insertion stoste plate
In；4th step in Fig. 1：Unload used pipette tips.
2.2nd, the motor driving piston rod on host header, by the DNA liquid extractions suction liquid transfer gun head of given dose.
2.3rd, host header Z axis moves upwards, pipette tips is departed from stoste, and is lifted to appropriate height so that motion process
Middle pipette tips will not be in contact with the plate tube in operating table surface.
Step 3：Liquid relief simultaneously quantitatively gets medium into specified pipe/plate：
The main purpose of this step is the DNA primer that will have been extracted or probe liquid is moved to the plate hole specified or centrifuge tube position
Put, and realize precisely getting for medium requirements dosage.
3.1st, linked by the slipway mechanism of X-direction and Y-direction, the host header for being loaded with DNA liquid is moved to accordingly
Above centrifuge tube or centrifugal hole.
3.2nd, moved downward by host header Z axis, liquid transfer gun head is inserted into corresponding plate hole or centrifuge tube.
3.3rd, the motor driving piston rod on host header, by the push of the DNA liquid of given dose get corresponding plate hole or
In centrifuge tube.
3.4th, host header Z axis moves upwards, and is lifted to appropriate height, is ready for subsequent motion.
, then need to be according to the phase of step 3.1 to 3.4 if necessary to which DNA primer or probe liquid are assigned in several pipe/holes
Same principle, the DNA liquid liquid relief and distribution for completing each specified pipe/hole are got, so as to fulfill once extracting, the mesh of porous distribution
's.
3.5th, as shown in Figure 1, host header links with the slipway mechanism of X-direction and Y-direction, it is moved to pipette tips separator 11
Put, pipette tips separator 11 blocks pipette tips, and host header moves upwards along Z axis.As shown in the 4th step in Fig. 1, used rifle is unloaded
Head, each primer or probe extracting, liquid relief and get can only a disposable pipette tips, to avoid there is cross contamination.
3.6th, host header moves to initial position, completes the packing or mixing liquid relief operation of a primer or probe.
According to the requirement for intending packing or mixing, there is primer/probe in several source plate hole/pipes to need to dispense or mix, just
Repeat above step two and step 3 several times.The present apparatus can be according to the complete parameter obtained in step 1, automatic linking
All operationss are completed, centre simultaneously occurs without pause, unless cause specific needs human intervention, can suspend by button, then use
When recovery button continue to run with.
Finally it should be noted that above content is merely illustrative of the technical solution of the present invention, rather than the present invention is protected
The limitation of scope, the simple modification or equivalent substitution that those of ordinary skill in the art carry out technical scheme,
All without departing from the spirit and scope of technical solution of the present invention.
Claims (9)
1. a kind of DNA primer or the accurate packing of probe and mixing arrangement, it is characterised in that：Precisely packing and the mixing arrangement
Mainly it is made of switch board, executing agency, working region component, test cell, pipette tips frame, working plate and stationary platen；Institute
Executing agency is stated to be made of X-axis slide unit, Y-axis slide unit, Z axis slide unit and rifle axis slide unit；In the negative direction upper table of the stationary platen
Face is fixedly connected with the switch board, the X-axis slide unit be fixed at the positive direction upper surface of the stationary platen and with institute
Switch board is stated to be fixedly connected；The working region component, the working region component are fixedly connected with the working plate
Adopted including 8 pieces of packing Target Boards, 1 block of pipette tips plate, 1 block of stoste plate, 1 piece of drain Target Board and 1 pipette tips separator, working plate
To the distribution form centered on stoste plate, 8 pieces of packing Target Boards are uniformly distributed in the both sides of stoste plate X-direction, positive direction 4
Block dispenses Target Board, and 4 pieces of packing Target Boards of negative direction, the pipette tips frame is arranged in the Y-axis positive direction of the stoste plate, described
Drain Target Board and the pipette tips separator are respectively arranged in the Y-axis negative direction of the stoste plate.
2. a kind of DNA primer according to claim 1 or the accurate packing of probe and mixing arrangement, it is characterised in that：Institute
State X-axis slide unit to be connected using planer type structure with Y-axis slide unit, the upper surface of X-axis slide unit is fixedly installed X-axis planker, drags in X-axis
It is bolted two root posts on plate to hold up Y-axis slide unit, the Z axis slide unit uses the structure shape of side lifting with Y-axis slide unit
Formula connects, and is fixedly installed Y-axis planker in the upper surface of Y-axis slide unit, the Z axis slide unit, institute are fixedly installed on Y-axis planker
State the side that rifle axis slide unit is arranged on the Z axis slide unit.
3. a kind of DNA primer according to claim 1 or 2 or the accurate packing of probe and mixing arrangement, it is characterised in that：
Internal controller, DC power supply, driver and dress centrally-mounted driving, described device driving motor are provided with the switch board
Drive the X-axis slide plate, Y-axis slide plate, Z axis slide plate and rifle axis skateboarding.
4. a kind of DNA primer according to claim 1 or 2 or the accurate packing of probe and mixing arrangement, it is characterised in that：
The surface of the packing target version is provided with 12 × 5 holes position, is provided with the upper right corner of each hole position for fixed centrifugation
The half elliptic step of pipe tube cover is raised.
5. a kind of DNA primer according to claim 1 or 2 or the accurate packing of probe and mixing arrangement, it is characterised in that：
The test cell is arranged on the upper surface of the working plate and in the Y-axis positive direction of the stoste plate.
6. a kind of DNA primer or the accurate packing of probe and the application method of mixing arrangement, it is characterised in that：Including dispensing or mixing
Demand parameter is closed to obtain step, Extraction medium step and liquid relief and quantitatively get Jie into specified pipe or plate from specified pipe or plate
Totally three steps, the specific operation process of each step are matter step：
Step 1: packing or the acquisition of mixing demand parameter：
1）In the selection of computer control interface and DNA synthesizer on-line operation or off-line operation；
2）Select packing pattern or mixed mode；
3）Read demand data, that is, the specific requirement for dispensing or mixing；
4）The combined operation that the demand parameter for dispensing or mixing is converted to each mechanism of device instructs；
Step 2: the Extraction medium from specified pipe or plate：
1）Host head unit links with the slipway mechanism of X-direction and Y-direction, is moved to above pipette tips frame；
2）Moved downward by host header Z axis, host header is fixed a liquid transfer gun head, then rise to appropriate height；
3）The host head unit of pipette tips has been plugged, has linked again with the slipway mechanism of X-direction and Y-direction, moves to stoste plate
Top, by moving downward for Z axis, make in the liquid in pipette tips insertion stoste plate corresponding hole site；
4）Motor driving piston rod on host header, by the DNA liquid extractions suction liquid transfer gun head of given dose；
5）Host header Z axis moves upwards, pipette tips is departed from stoste, and is lifted to appropriate height so that pipette tips in motion process
It will not be in contact with the plate in operating table surface or pipe；
Step 3: liquid relief and quantitatively getting medium into specified pipe or plate：
1）Linked by the slipway mechanism of X-direction and Y-direction, the host header for being loaded with DNA liquid is moved to corresponding centrifuge tube
Or above centrifugal hole；
2）Moved downward by host header Z axis, liquid transfer gun head is inserted into corresponding plate hole or centrifuge tube；
3）Motor driving piston rod on host header, corresponding plate hole or centrifuge tube are got by the DNA liquid push of given dose
It is interior；
4）Host header Z axis moves upwards, and is lifted to appropriate height, is ready for subsequent motion；
5）Host header links with the slipway mechanism of X-direction and Y-direction, is moved to pipette tips separator position, pipette tips separator blocks
Pipette tips, host header move upwards along Z axis, unload used pipette tips；
6）Host header moves to initial position, completes the packing or mixing liquid relief operation of a primer or probe.
7. a kind of DNA primer according to claim 6 or the accurate packing of probe and the application method of mixing arrangement, it is special
Sign is：In the step 1, when precisely packing and mixing arrangement are with DNA synthesizer on-line operation, synthesize and digitize in DNA
In management system, inputted according to the demand of customer order, device is real using digital module collection compatible and management system
Existing demand data is introduced directly into.
8. a kind of DNA primer according to claim 6 or the accurate packing of probe and the application method of mixing arrangement, it is special
Sign is：In the step 1, when precisely packing and mixing arrangement are with DNA synthesizer off-line operation, the directly control in device
Computer processed treats that operating file presss from both sides and establishes packing or mixing demand information, including intends position, the body dispensed or blending agent is initial
Product and the positional information that will be transferred to.
9. a kind of DNA primer according to claim 6 or the accurate packing of probe and the application method of mixing arrangement, it is special
Sign is：According to the requirement for intending packing or mixing, there is the medium in several source plate holes to need to dispense or mix, just repeat the step
Rapid two and step 3 several times.
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| CN201711270989.8A CN107916222B (en) | 2017-12-05 | 2017-12-05 | Accurate split charging and mixing device for DNA primers or probes and application method thereof |
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| CN201711270989.8A CN107916222B (en) | 2017-12-05 | 2017-12-05 | Accurate split charging and mixing device for DNA primers or probes and application method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109321429A (en) * | 2018-09-28 | 2019-02-12 | 农业部沼气科学研究所 | A kind of automatical bacterium instrument and microbe to screen method |
| CN109433094A (en) * | 2018-11-07 | 2019-03-08 | 深圳大学 | Liquid dispensing device |
| WO2021174925A1 (en) * | 2020-03-06 | 2021-09-10 | 苏州金唯智生物科技有限公司 | Ammonolysis purifier |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101529246A (en) * | 2005-12-21 | 2009-09-09 | 梅索斯卡莱科技公司 | Assay apparatuses, methods and reagents |
| WO2011140071A1 (en) * | 2010-05-03 | 2011-11-10 | Integra Biosciences Corp. (Formerly Viaflo Corporation) | Pipette tip positioning for manually-directed, multi-channel electronic pipettor |
| CN202845059U (en) * | 2012-10-16 | 2013-04-03 | 杭州爱菩高特生物技术有限公司 | Multifunctional centrifuge tube box with cover |
| CN103484363A (en) * | 2013-10-10 | 2014-01-01 | 北京中纳海润生物科技有限公司 | Automatic purification and separation system |
| CN204298409U (en) * | 2014-12-03 | 2015-04-29 | 深圳华大基因研究院 | For the sample pretreatment equipment of gene sequencing system |
| CN106085817A (en) * | 2016-07-28 | 2016-11-09 | 杭州杰毅麦特医疗器械有限公司 | The device of a kind of subpackage micro liquid and method for filling thereof |
| CN106367311A (en) * | 2016-12-08 | 2017-02-01 | 湖南圣湘生物科技有限公司 | Nucleic acid extraction system and using method thereof |
| CN106840797A (en) * | 2017-01-24 | 2017-06-13 | 上海析维医疗科技有限公司 | The automatic preparation work station and compound method of a kind of standard liquid |
| CN207581826U (en) * | 2017-12-05 | 2018-07-06 | 上海百力格生物技术有限公司 | The accurate packing of a kind of DNA primer or probe and mixing arrangement |
-
2017
- 2017-12-05 CN CN201711270989.8A patent/CN107916222B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101529246A (en) * | 2005-12-21 | 2009-09-09 | 梅索斯卡莱科技公司 | Assay apparatuses, methods and reagents |
| WO2011140071A1 (en) * | 2010-05-03 | 2011-11-10 | Integra Biosciences Corp. (Formerly Viaflo Corporation) | Pipette tip positioning for manually-directed, multi-channel electronic pipettor |
| CN202845059U (en) * | 2012-10-16 | 2013-04-03 | 杭州爱菩高特生物技术有限公司 | Multifunctional centrifuge tube box with cover |
| CN103484363A (en) * | 2013-10-10 | 2014-01-01 | 北京中纳海润生物科技有限公司 | Automatic purification and separation system |
| CN204298409U (en) * | 2014-12-03 | 2015-04-29 | 深圳华大基因研究院 | For the sample pretreatment equipment of gene sequencing system |
| CN106085817A (en) * | 2016-07-28 | 2016-11-09 | 杭州杰毅麦特医疗器械有限公司 | The device of a kind of subpackage micro liquid and method for filling thereof |
| CN106367311A (en) * | 2016-12-08 | 2017-02-01 | 湖南圣湘生物科技有限公司 | Nucleic acid extraction system and using method thereof |
| CN106840797A (en) * | 2017-01-24 | 2017-06-13 | 上海析维医疗科技有限公司 | The automatic preparation work station and compound method of a kind of standard liquid |
| CN207581826U (en) * | 2017-12-05 | 2018-07-06 | 上海百力格生物技术有限公司 | The accurate packing of a kind of DNA primer or probe and mixing arrangement |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109321429A (en) * | 2018-09-28 | 2019-02-12 | 农业部沼气科学研究所 | A kind of automatical bacterium instrument and microbe to screen method |
| CN109321429B (en) * | 2018-09-28 | 2020-09-08 | 农业部沼气科学研究所 | Automatic bacterium picking instrument and microorganism screening method |
| CN109433094A (en) * | 2018-11-07 | 2019-03-08 | 深圳大学 | Liquid dispensing device |
| CN109433094B (en) * | 2018-11-07 | 2024-03-29 | 深圳大学 | Liquid dispensing device |
| WO2021174925A1 (en) * | 2020-03-06 | 2021-09-10 | 苏州金唯智生物科技有限公司 | Ammonolysis purifier |
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| Publication number | Publication date |
|---|---|
| CN107916222B (en) | 2023-10-10 |
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