WO2021174925A1

WO2021174925A1 - Ammonolysis purifier

Info

Publication number: WO2021174925A1
Application number: PCT/CN2020/132643
Authority: WO
Inventors: 张一华; 孙杭桂; 方其; 张艳
Original assignee: 苏州金唯智生物科技有限公司
Priority date: 2020-03-06
Filing date: 2020-11-30
Publication date: 2021-09-10
Also published as: CN212293573U

Abstract

The present application provides an ammonolysis purifier. The ammonolysis purifier comprises: a platform, a liquid separation assembly, a perforated synthetic plate placing table, a positive pressure assembly, and a recycle assembly. The liquid separation assembly is mounted on the platform and provided with a liquid separator having multiple pin holes. The perforated synthetic plate placing table is configured to place a perforated synthetic plate; and the perforated synthetic plate placing table is provided on sliding rails, so that the perforated synthetic plate on the perforated synthetic plate placing table is located below the liquid separator of the liquid separation assembly or located below the positive pressure assembly. The positive pressure assembly is mounted on the platform and comprises: a sealing cover plate capable of being mounted on the perforated synthetic plate placing table in a covering manner, and a pressurizing member capable of applying a positive pressure into a sealed space formed by the sealing cover plate and the perforated synthetic plate placing table. The recycle assembly comprises a horizontal transmission member and a moving table configured to place a material receiving plate and a waste liquid box, wherein the moving table can move along the horizontal transmission member, so that the material receiving plate or the waste liquid box is located below the positive pressure assembly.

Description

Ammonialysis Purification Apparatus

This application claims the priority of the Chinese patent application whose application date is March 6, 2020 and the application number is 202020267225.4. The entire content of this application is incorporated into this application by reference.

Technical field

This application belongs to the field of biomedical testing equipment, for example, it relates to an ammoniolysis purification instrument.

Background technique

In the biological industry, ammoniolysis purification is the most common application experiment. Small batches are completely operated manually. When batches of samples require ammoniolysis and purification, if they still rely on manual labor, it will consume a lot of manpower and efficiency. low. When adding samples manually, problems such as visual fatigue, error operation, etc. that affect the purity of the primers will occur, and the final result cannot be unified. There is no similar equipment in the related technology for the ammonia hydrolysis purifier, and it mainly depends on manual operation.

Summary of the invention

The present application provides an ammoniolysis purification instrument, which can solve the technical problems of low efficiency and large errors in the manual implementation of a large number of ammonification purification experiments in related technologies.

An embodiment provides an ammoniolysis purification instrument, which includes a platform, a liquid separation component, a perforated synthetic plate placing table, a positive pressure component, and a recovery component.

The liquid separating assembly is installed on the platform, and the liquid separating assembly is provided with a liquid separator with a plurality of pinholes.

The perforated composite plate placing table is set to place the perforated composite plate, and the perforated composite plate placing table is set on the slide rail so that the perforated composite plate on the perforated composite plate placing table is located in the liquid separating component Under the device or under the positive pressure component.

The positive pressure component is installed on the platform, and the positive pressure component includes a sealing cover plate capable of being mounted on the perforated composite plate placing table, and a sealing cover plate and the perforated composite plate placing platform can be formed. A pressurizing member that applies positive pressure in the enclosed space.

The recycling assembly includes a horizontal transfer member, and a mobile station configured to place a material receiving plate and a waste liquid box. The mobile station can move along the horizontal transfer member so that the material receiving plate or the waste liquid box is located at the bottom of the positive pressure assembly. Below.

Description of the drawings

The technical solution of the present application will be further described below with reference to the drawings and embodiments.

Fig. 1 is a front view of an ammonolysis purification apparatus according to an embodiment of the present application;

Fig. 2 is a top view of an ammonolysis purification apparatus according to an embodiment of the present application;

Fig. 3 is a partial top view of an ammonolysis purification apparatus according to an embodiment of the present application;

Fig. 4 is a side view of an ammonolysis purification apparatus according to an embodiment of the present application.

1. Frame;

2. Dispensing components;

3. Placement table for composite board with holes;

4. Positive pressure components;

5. Recycling components;

9. Composite board with holes;

11. Platform;

12. Shell;

13. Exhaust port;

14. Reagent barrel;

15. Waste liquid barrel;

16. Display board;

21. Dispenser;

41. Sealing cover plate;

42. Lower airtight cylinder;

43. Upper airtight cylinder;

44. Locking parts;

51. Conveying roller;

52. Material receiving board;

53. Waste liquid box;

81. Operation panel;

82. Electric control box.

Detailed ways

It should be noted that the embodiments in the application and the features in the embodiments can be combined with each other if there is no conflict.

In the description of this application, it should be understood that the terms "center", "vertical", "horizontal", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the application and The description is simplified, rather than indicating or implying that the pointed device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the protection scope of the present application. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Thus, the features defined with "first", "second", etc. may explicitly or implicitly include one or more of these features. In the description of the creation of the present utility model, unless otherwise specified, "multiple" means two or more than two.

In the description of this application, it should be noted that the terms "installation", "connection", and "connection" should be understood in a broad sense, unless otherwise clearly specified and limited. For example, it can be a fixed connection or a detachable connection. Connected or integrally connected; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal communication between two components. For those of ordinary skill in the art, the specific meaning of the above-mentioned terms in this application can be understood through specific circumstances.

The technical solutions of the present application will be described in detail below with reference to the drawings and in conjunction with the embodiments.

Example one

This embodiment provides an aminolysis purification apparatus, as shown in FIG. 1, including: a platform 11, which is usually located in the middle of the frame 1. The frame 1 is enclosed by a shell 12 formed by plates, doors, and windows. The top of 12 has an exhaust port 13, which is connected with an exhaust fan to ensure that the gas inside the housing 12 is discharged in time. The bottom space of the platform 11 is set to place reagent barrels 14 and waste liquid barrels 15. There are usually multiple reagent barrels 14 , The part above the platform 11 is provided with the following components; the liquid dispensing assembly 2, the positive pressure module 4 and the recovery module 5. 21; The perforated composite board placement table 3 is set to place the perforated composite board 9, and the perforated composite board placement platform 3 is set on the X-axis slide rail so that the perforated composite board is placed on the perforated composite board on the platform 3 9 is located below the dispenser of the dispensing assembly 2 or below the positive pressure assembly 4; the positive pressure assembly 4 is installed on the platform 11, and the positive pressure assembly 4 includes a seal that can be covered and mounted on the perforated composite plate placing table 3 The cover plate 41, and a pressurizing member capable of applying positive pressure to the enclosed space formed by the sealing cover plate 41 and the perforated composite plate placing table 3; the recycling assembly 5 includes a horizontal transmission member and a material receiving plate 52 and a waste liquid. The moving table of the box 53, the horizontal transfer member can be set as a transfer roller 51, and the moving table can move along the horizontal transfer member so that the material receiving plate 52 or the waste liquid box 53 is located below the positive pressure assembly 4. When the receiving plate 52 is located under the positive pressure component 4, it can receive the processed reagents flowing down from the perforated synthetic plate 9. The material receiving plate 52 is arranged opposite to the perforated synthetic plate 9 with the same number of holes, but blind holes, perforated synthetic plate The hole on 9 has an opening at the top for reagent injection, and the bottom end can allow reagents to pass under pressure. The waste liquid box 53 can receive waste flowing down from the perforated synthetic plate 9 when it is located under the positive pressure assembly 4 liquid.

The ammoniolysis purification instrument provided in this application, when used, the perforated synthesis plate placing table 3 drives the perforated synthesis plate 9 to the bottom of the separator of the liquid separation assembly 2, so that the perforated synthesis plate 9 receives the output from the liquid separation assembly 2. Liquid reagents are injected into the holes of the synthetic plate 9 with holes. Then the perforated composite plate placing table 3 drives the perforated composite plate 9 to the bottom of the positive pressure component 4, and the mobile recycling component 5 reaches below the perforated composite plate placing table 3, and the positive pressure component 4 places the perforated composite plate 3 together with the perforated The composite plate 9 is sealed together, and the pressure is applied to the perforated composite plate 9 by the pressurizing member. Under the pressure, the processed reagent flows into the material receiving plate 52 below, and is transported away by the material receiving plate 52, and the waste liquid box 53 It reaches the bottom of the perforated synthetic board placement platform 3 to receive the waste liquid, and the waste liquid finally flows into the waste liquid bucket 15 through the pipe and the pump.

Above the platform 11 is also provided a display panel 16 on which a dial device such as a pressure gauge is installed. The perforated synthetic plate placement table 3 drives the perforated synthetic plate 9 to move under the dispensing assembly 2 and the positive pressure assembly 4. When the perforated synthetic plate 9 is under the dispensing assembly 2, the reagents are removed from the reagent tank 14 by the dispensing assembly 2 The medium is drawn out and filled on the synthetic plate 9 with holes. The reagent is liquid. The setting of the liquid pipeline, valve and pump refers to the related technology. How to realize the process of the reagent from the reagent barrel 14 to the dispensing assembly 2 is not repeated here. The recovery component 5 is configured to recover waste liquid or primers flowing out of the perforated synthetic plate 9.

In an embodiment, the horizontal transmission member extends to the outside of the housing 12, and the material receiving plate 52 can follow the horizontal transmission member to the outside of the housing 12 to facilitate loading and unloading of the material receiving plate 52.

In an embodiment, the well-perforated synthetic plate placement table 3 can accommodate two well-equipped synthetic plates 9 arranged side by side, and the two well-perfected synthetic plates 9 are respectively a 96-well synthetic plate and a 384-well synthetic plate. The two kinds of synthetic plates 9 with holes can be adapted to 96-well synthetic plates and 384-well synthetic plates at the same time.

In one embodiment, the recovery component 5 can be moved under the 96-well synthesis plate and the 384-well synthesis plate, so as to realize that one recovery component 5 is adapted to the 96-well synthesis plate and the 384-well synthesis plate at the same time, and is located under the 96-well synthesis plate. At the time, accept the waste liquid from the 96-well synthesis plate, and when it is located under the 384-well synthesis plate, accept the waste liquid from the 384-well synthesis plate.

The waste liquid box 53 is in the shape of a funnel. The waste liquid flowing out of the perforated synthetic plate 9 or the waste liquid after cleaning is first collected by the waste liquid box 53 and then flows into the waste liquid bucket 15 which passes through the pipe, The valve and the pump are connected to the bottom of the waste liquid box 53, and the connection mode can be referred to related technologies, which will not be repeated here.

In an embodiment, the dispenser 21 of the dispensing assembly 2 includes a dispensing plate with a plurality of mounting holes, and a dispensing needle is installed on each mounting hole, and each dispensing needle can pass through reagents and dispense. The arrangement and number of liquid needles are set corresponding to the well-equipped synthesis plate 9, and the reagents are introduced into the corresponding reagent wells on the well-equipped synthesis plate 9. In order to be compatible with the 96-well synthesis plate and the 384-well synthesis plate, the separation plates are arranged in a row 8 wells, a total of 12 rows are a small plate, the small plate directly corresponds to the 96-well synthetic plate, and the combination of 4 small plates can be compatible with the 384-well synthetic plate.

When reagents are added, the liquids of different reagents are separated to avoid cross-contamination between reagents. After the liquid is added, the reagents can be fully combined with the cyanoethyl groups and other impurities on the column by pressurizing them. It can better remove the cyanoethyl group and impurities, avoid manual and repeated taking, and save operation and time costs. Secondly, regarding the reagent squeezing function of the ammoniolysis purifier, the removal of liquid after adding can rely on the squeezing function, while the related technology completely relies on the centrifuge, and each step of the operation must be centrifuged, so the ammoniolysis purifier of this application can get rid of It depends on the centrifuge and ensures that the liquid will not overflow. Since the liquid overflow may damage the centrifuge during the centrifugation process, the ammoniolysis purifier of this embodiment can avoid the influence of the liquid overflow on the equipment.

In one embodiment, the pressurizing member is an inflating air nozzle arranged on the sealing cover 41, the inflating air nozzle is connected with an air pump to apply pressure to the enclosed space, and the sealing cover 41 is also provided with a deflation air nozzle , After opening the vent nozzle, it can break the positive pressure environment in the confined space.

In one embodiment, the first side of the sealing cover 41 is hingedly arranged, the second side of the sealing cover 41 is provided with a locking member 44, and an upper airtight cylinder 43 is used to lock the sealing cover 41 The stop and airtight cover is placed on the perforated composite plate placing table 3, the sealing cover 41 and the perforated composite plate placing table 3 are sealed by rubber, and the lower airtight cylinder 42 can lift up the material receiving plate 52 It is sealed with the perforated composite plate placing table 3, and the airtight space is formed by the action of the upper airtight cylinder 43 and the lower airtight cylinder 42, and then the air is pumped into the airtight space through the inflation nozzle to increase the airtight space pressure. It is pressed onto the material receiving plate 52 from the perforated composite plate placing table 3.

The waste liquid box 53 opens upward and receives waste liquid leaked from the perforated synthetic plate 9. In an embodiment, the recovery assembly 5 further includes a waste fluid channel connecting the waste fluid box 53 and the exterior of the ammonolysis purifier. When the positive pressure assembly 4 squeezes the reagent, the waste fluid is discharged from the waste fluid channel. The recovery assembly 5 can move simultaneously with the perforated composite plate 9. The function is to catch the perforated synthetic plate 9 in the process of ammonolysis or purification, and there may be liquid leakage in the process of liquid separation, and to discharge the waste liquid from the waste liquid channel when the reagent is positively pressured.

In an embodiment, the liquid dispensing assembly 2 is also connected to a washing assembly, and the washing assembly includes a washing solution container, a water pipe, and a valve on the water pipe configured to connect the washing solution container to the washing solution container. The valve adjusts the water flow rate. The water pipe cleaning liquid distribution component 2 is equipped with a liquid level detector in the washing liquid container, and the liquid level detector is connected with a low liquid level alarm. The water washing function added in the auxiliary function ensures that the reagent in the pipeline is cleaned, avoids the blockage of the pipeline, and maintains the good condition of the equipment. In addition to the above functions, the ammonia hydrolysis purification instrument also adds a liquid level detection device. If the liquid level is too low, an alarm function will be activated. Alternatively, the reagent barrel is replaced with a reagent barrel containing cleaning agent, and the cleaning agent flows through the liquid separating assembly 2 to clean the liquid separating assembly 2.

In one embodiment, sensors are added to several places of the ammonia hydrolysis purification instrument. When the ammonia synthesis purification instrument detects an abnormality, it will automatically alarm and give a prompt, which greatly guarantees the safety of the ammonia synthesis purification instrument.

The added additional functions can effectively protect the equipment and personnel, the cleaning of the pipeline and the detection of the liquid level can effectively protect the equipment, and the prompt and even emergency stop function after an error can effectively protect the personnel.

In one embodiment, a reagent barrel 14, a reagent barrel 14 and a waste liquid barrel 15 are placed under the platform 11. The reagent barrel 14 is often contained in a box, and the waste liquid barrel 15 is in communication with the waste liquid box 53 of the recycling assembly 5. The reagent barrels 14 contain reagents to be purified. Each reagent barrel 14 contains one type of reagent. As many reagent barrels 14 are needed as there are reagents. The reagent barrels 14 are connected to the dispenser of the liquid separation assembly 2 through a pipe. The waste liquid bucket 15 is configured to store waste liquid, and other accessory parts and other accessories can also be stored under the platform 11 to ensure beauty while ensuring safety.

Considering factors such as safety, in one embodiment, the platform 11 and its upper and lower components are arranged in a housing 12. External and control components such as an electric control box 82 can be provided on the housing 12. An exhaust port 13 is also provided on the top of the housing 12, and the exhaust port 13 is used to keep the inside of the device clean. An electric control box 82 is also provided on the housing 12, and the electric control box 82 is in electrical communication with and controls the dispenser and the pressing tool to evenly divide and squeeze the reagent to be purified.

In order to facilitate human-computer interaction, in one embodiment, the ammonia hydrolysis purification instrument further includes an operation panel 81 on the housing 12, and the operation panel 81 is provided with management authority that can set the operating parameters of the ammonia purification purification instrument and cannot set the ammonia purification purification instrument. Operation authority for operating parameters. This allows managers to make different settings for equipment and independently design operating procedures based on experiments. The operator can only operate according to the previous settings to ensure that various parameters are not modified, which ensures the safety of the equipment.

The beneficial effects of this application are: the ammonolysis purifier uses a variety of mechanical devices to perform all operations by the equipment, reducing manpower and improving efficiency; the separation of multiple channels for different liquids can avoid cross-contamination between reagents and liquids. After adding, the reagent can be fully combined with the cyanoethyl group and other impurities on the column by shifting and adding a small pressure, which can better remove the cyanoethyl group and impurities, avoiding repeated manual picking and saving Operation and time costs; the removal of liquid after adding can rely on the squeezing function, get rid of the dependence on the centrifuge, and ensure that the liquid will not overflow.

Claims

An ammoniolysis purification instrument, including:

Platform (11);

The liquid distributing component (2) is installed on the platform (11), and the liquid distributing component (2) is provided with a liquid distributor with a plurality of pinholes;

The perforated composite plate placing table (3) is set to place the perforated composite plate (9), and the perforated composite plate placing table (3) is set on the slide rail so that the perforated composite plate placing table (3) is The perforated composite plate (9) is located below the dispenser of the dispensing assembly (2) or under the positive pressure assembly (4);

The positive pressure component (4) is installed on the platform (11), and the positive pressure component (4) includes a sealing cover plate (41) that can be installed on the perforated composite plate placing table (3), And a pressurizing member capable of applying positive pressure to the enclosed space formed by the sealing cover plate (41) and the perforated composite plate placing table (3);

The recycling assembly (5) includes a horizontal transfer member, and a mobile station arranged to place the material receiving plate (52) and the waste liquid box (53), and the mobile station can move along the horizontal transfer member to make the material receiving plate ( 52) Or the waste liquid box (53) is located under the positive pressure component (4).
The ammoniolysis purification apparatus according to claim 1, wherein the perforated synthetic plate placing table (3) can accommodate two perforated synthetic plates (9) arranged side by side, and the two perforated synthetic plates (9) are respectively There are two 96-well synthetic plates, two 384-well synthetic plates or one 96-well synthetic plate and one 384-well synthetic plate.
The ammoniolysis purification instrument according to claim 2, wherein a material receiving plate (52) corresponding to a 96-well synthetic plate or a 384-well synthetic plate is provided on the mobile platform.
The ammoniolysis purification instrument according to claim 1, wherein the dispenser (21) of the fluid separating assembly (2) comprises a fluid separating plate having a plurality of mounting holes, and a fluid dispensing needle is installed on each mounting hole , Each dispensing needle can pass the reagent and introduce the reagent into the corresponding reagent hole on the perforated synthetic plate (9).
The ammoniolysis purification apparatus according to claim 4, wherein the horizontal transmission member can be extended to the outside of the housing (12), and the material receiving plate (52) is arranged to follow the horizontal transmission member to the outside of the housing (12).
The ammonolysis purifier according to claim 1, wherein the pressurizing member is an inflatable gas nozzle arranged on the sealing cover plate (41), and the inflatable gas nozzle is connected with the air pump to apply pressure to the enclosed space, and the sealing cover The plate (41) is provided with a deflation nozzle. After the deflation nozzle is set to open, the positive pressure environment in the enclosed space is broken.
The ammoniolysis purification apparatus according to claim 1, wherein the first side of the sealing cover (41) is hingedly arranged, and the second side of the sealing cover (41) is provided with a locking member to prevent The sealing cover plate (4) is locked on the perforated composite plate placing table (3).
The ammoniolysis purification apparatus according to claim 1, wherein the platform (11) is located in the middle of the frame (1), and the frame (1) is enclosed by a shell (12) formed by plates, doors and windows.
The ammonolysis purifier according to claim 8, further comprising at least one reagent barrel (14) and at least one waste liquid barrel (15), the bottom of the platform (11) has a bottom space, the reagent barrel (14) and The waste liquid bucket (15) is arranged in the bottom space.
The ammoniolysis purification apparatus according to claim 7, wherein the horizontal transmission member can be extended to the outside of the housing (12), and the material receiving plate (52) is arranged to follow the horizontal transmission member to the outside of the housing (12), so that Load and unload materials on the material receiving board (52).