CN207581826U - The accurate packing of a kind of DNA primer or probe and mixing arrangement - Google Patents

Abstract

Accurate packing and mixing arrangement the utility model discloses a kind of DNA primer or probe, this is precisely dispensed and mixing arrangement is mainly made of switch board, executing agency, working region component, test cell, pipette tips frame, working plate and stationary platen；Executing agency is made of X-axis slide unit, Y-axis slide unit, Z axis slide unit and rifle axis slide unit；Working region component includes 8 pieces of packing Target Boards, 1 block of pipette tips plate, 1 block of stoste plate, 1 piece of drain Target Board and 1 pipette tips separator, working plate and uses the distribution form centered on stoste plate.The utility model provides a kind of accurate, reliable, efficient engineered solution, is precisely dispensed so as to fulfill the automation of DNA primer or probe with mixing.The utility model can be saved and manually, can save cumbersome manual imbibition and beat liquid process, and operator only needs that required operation is imported software using mobile USB flash disk, and the preparation before eliminating realizes full-automatic operation.

Description

The accurate packing of a kind of DNA primer or probe and mixing arrangement

Technical field

The utility model belongs to technical field of molecular biology, particularly relates to the essence of a kind of DNA primer or probe Quasi- packing and mixing arrangement.

Background technology

In molecular biosciences research, according to specific purpose, need to synthesize the primer of different bases sequence and length.DNA draws Object in post synthesis, is generally in the orifice plate of standard, such as 48 orifice plates or 96 orifice plates, can be one different in each hole Primer.Purpose according to analysis and research is different, it is sometimes desirable to will be synthetic same（Referred to as one）Primer or probe, It is dispensed into several different centrifuge tubes or screwed pipe, needs different primer or probe being mixed into same pipe again sometimes In son, the usually work of these packing or mixing is had been manually done by operating personnel by liquid-transfering gun.Complete different primers in 96 holes The packing of unequal number amount mixes different primers according to different quantity, an inherently very complicated work Make.Since it is desired that packing or mixing primer or probe with microlitre measure, it is very high to the skill set requirements of operating personnel, be often difficult to By measurement deviation or undulated control in smaller range.Meanwhile manual operations can neither avoid human error completely, and poor in appearance Stagger the time is difficult to be found.

Laboratory liquor removing workstation or instrument for extracting nucleic acid of the prior art are only capable of realizing certain liquid from a kind of container Amount is transferred to the purpose of another container, can not realize primer or probe packing, mixing complexity and precision requirement.It is counted Amount unit is usually milliliter magnitude, and measuring accuracy is 1% or so, and the packing or mixing of primer or probe, measurement unit are micro- Rising amount grade, measuring accuracy requirement higher, laboratory liquor removing workstation or instrument for extracting nucleic acid can not meet the height of this micro-measurement Precision and high-stability requirement.And laboratory liquor removing workstation or instrument for extracting nucleic acid can not carry out realizing the automatic of data module Acquisition, can not realize the compatible and connection with DNA primer or probe synthesis system.

Invention content

For the utility model in order to overcome the shortcomings of the prior art, it is big with mixing two that the utility model aims at packing The device of function provides accurate packing and the mixing arrangement of a kind of DNA primer or probe.

The utility model is achieved through the following technical solutions：The accurate packing and mixing of a kind of DNA primer or probe Device, mainly by switch board, executing agency, working region component, test cell, pipette tips frame, working plate and stationary platen Composition；Executing agency is made of X-axis slide unit, Y-axis slide unit, Z axis slide unit and rifle axis slide unit；In the negative direction upper surface of stationary platen Switch board is fixedly connected with, X-axis slide unit is fixed at the positive direction upper surface of stationary platen and is fixedly connected with switch board； Be fixedly connected with working region component on working plate, working region component include 8 pieces of packing Target Boards, 1 block of pipette tips plate, 1 piece Stoste plate, 1 piece of drain Target Board and 1 pipette tips separator, working plate use the distribution form centered on stoste plate, 8 pieces Packing Target Board is uniformly distributed in the both sides of stoste plate X-direction, 4 pieces of packing Target Boards of positive direction, 4 pieces of packing targets of negative direction Plate, pipette tips frame are arranged in the Y-axis positive direction of stoste plate, and drain Target Board and pipette tips separator are respectively arranged in the Y of stoste plate In axis negative direction.

X-axis slide unit is connect with Y-axis slide unit using planer type structure, and the upper surface of X-axis slide unit is fixedly installed X-axis planker, It is bolted two root posts on X-axis planker to hold up Y-axis slide unit, Z axis slide unit uses the structure of side lifting with Y-axis slide unit Form connects, and is fixedly installed Y-axis planker in the upper surface of Y-axis slide unit, Z axis slide unit, rifle axis are fixedly installed on Y-axis planker Slide unit is arranged on the side of Z axis slide unit.

Internal controller, DC power supply, driver and device driving motor, device driving motor are provided in switch board Drive X-axis slide plate, Y-axis slide plate, Z axis slide plate and rifle axis skateboarding.The surface of packing target version is provided with 12 × 5 holes position, The half elliptic step protrusion for fixing centrifuge tube pipe lid is provided in the upper right corner of each hole position.Test cell is arranged on The upper surface of working plate is simultaneously located in the Y-axis positive direction of stoste plate.

In medicine and biology research and production, according to the purpose of certain medical treatment detection, diagnosis and research and development, there is difference The primer or probe of base sequence and length are devised, and after the processes such as molecule synthesis, ammonolysis and purifying, finished product is deposited It is stored in orifice plate or centrifuge tube.Then it and according to specific demand and is dispensed or is mixed.

1st, it dispenses：

General dna primer finished product, is generally in the orifice plate of standard, such as 48 orifice plates or 96 orifice plates, each Kong Zhongke It can be a special primer of the different demands from different clients.It is collected according to the specific requirement of client, in each hole or temporary The every primer deposited needs the several pieces for being divided into determining dosage, packs and be stored in centrifuge tube, referred to as " plate is to pipe " point Dress process.

DNA probe or certain primer finished products by special purifying process are usually collected and are temporarily stored into larger centrifuge tube, According to the specific requirement of client, the several pieces for being divided into determining dosage are needed, packs and is stored in smaller centrifuge tube, referred to as Process is dispensed for " pipe is to pipe ".

In order to subsequently mix purpose, the primer being temporarily stored into orifice plate or the primer being temporarily stored into larger centrifuge tube or spy Needle is needed to be divided into several pieces according to certain dosage and share, is temporarily stored into the designation hole of 96 orifice plate of standard.Both were dispensed Journey, the former is referred to as " plate is to plate " packing process, and the latter is referred to as " pipe is to plate " packing process.

2nd, it mixes：

Based on medicine and the purpose of biological study, it is sometimes desirable to by the different primers in 96 orifice plates or centrifuge tube or Probe is mixed according to certain dosage.Similar with packing process, the process of mixing can also be " plate is to plate ", " pipe pair The mixed process of plate ", " plate is to pipe " or " pipe is to pipe ".In general, if primary need to complete the mixing compared with multiple types, and mixing It dispenses again afterwards or continues to test, then it is final to mix more in 96 orifice plates；If only has a few, and after mixing no longer It needs subsequently to dispense, then can directly mix into centrifuge tube.

DNA primer --- primer（primer）Also known as introduction.It is a bit of single stranded DNA or RNA, as rising for DNA replication dna Initial point, in nucleic acid synthetic reaction, the polynucleotide chain that works as the starting point that each polynucleotide chain is extended, On 3 '-OH of primer, nucleotide is synthesized in the form of diester chain, therefore 3 '-OH of primer must be free.

DNA probe --- gene probe, i.e. nucleic acid probe（probe）, it is one section and carries known to detection label and sequence , with the nucleic acid sequence (DNA or RNA) of target gene complementation.

48 orifice plates or 96 orifice plates --- in scientific researches such as biologic medicals with being widely used in production field, as cell culture apparatus Ware, quantitative fluorescent PCR platform consumptive material or nucleic acid collect temporary ware etc..

Centrifuge tube --- tubulose sample container, can with closure or gland, for storing synthetic primer or probe.

Accurate packing and mixing arrangement and the liquor removing workstation or nucleic acid of medical laboratory in the utility model carry Take instrument more similar.With reference to primer or probe material performance characteristics in itself, packing or the particular/special requirement of mixing, control accuracy etc. Aspect, the device in the utility model provide the designing scheme of a variety of novelties, realize laboratory liquor removing workstation or The function and solution that instrument for extracting nucleic acid can not be realized.

The beneficial effects of the utility model are：The utility model DNA primer or the mesh of the accurate packing of probe and mixing arrangement Be just to provide a kind of accurate, reliable, efficient engineered solution, precisely divided with the automation for realizing DNA primer or probe Dress is with mixing.Using the device in the utility model, can save manually, which can save cumbersome manual imbibition and beat Liquid process, operator only need that required operation is imported software, the beam worker before eliminating using mobile USB flash disk Make.Device complete work safety and stability it is significantly larger than artificial, will not because of it is artificial the reason of there is mistake.The present apparatus Precision can reach within 2%, and can 30min or so complete one piece packing Target Board packing operation, realize high-precision The packing of wide range and liquid relief work.The present apparatus can realize full-automatic operation, by the integration of device, can also conveniently after The integration of the whole process automation of phase.

The present apparatus employs the template setting of eight pieces of Target Boards of brand-new design, can realize the big portion of DNA primer packing The division of labor is required, and the operations such as plate are changed without midway pause.One-time pad can disposably complete the packing work of 96 primers, greatly The big accuracy and speed for improving primer packing liquid relief, improves and purchases efficiency, ensure that the consistency and reliability purchased.This Utility model can complete primer or probe from extracting to dispensing or the accurate measurement that mixes.Motion process is stable, reliable, positioning Accurately.Precisely the advantage of packing and mixing arrangement is the utility model：Slotting pipette tips, imbibition are realized, beat liquid and converts Kong Weizai It is secondary to beat the functions such as liquid, it is at best able to five pipe of packing.

Description of the drawings

Fig. 1 is the structure diagram using the utility model device Extraction medium step from specified pipe/plate；

Fig. 2 is the dimensional structure diagram of the utility model packing and mixing arrangement；

In Fig. 2：1- switch boards；2-X axis slide units；3-Y axis slide units；4-Z axis slide units；5- rifle axis slide units；6- test cells；7- Dispense Target Board；8- pipette tips framves；9- stoste plates；10- drain Target Boards；11- pipette tips separators；12- working plates；13- is fixed Platen；14- holes position；15- half elliptics step protrusion.

Fig. 3 is the control system hardware architecture diagram of the utility model packing and mixing arrangement；

Fig. 4 is the connection mode of the utility model executing agency and control principle schematic diagram；

Fig. 5 is the dimensional structure diagram that Target Board is dispensed in the utility model packing and mixing arrangement；

Wherein：Switch board installs all control sections, and test cell is used for realizing the test for beating liquid measure to device, packing Target Board is used for installing the primer after packing, and pipette tips frame is used for installing liquid transfer gun head for pending trial, and stoste plate, which is used for installing, to be treated point The original primers of dress.

Specific embodiment

The utility model is described in detail below in conjunction with the drawings and specific embodiments.

As shown in Figures 1 to 5, the accurate packing of a kind of DNA primer or probe and mixing arrangement, mainly by switch board 1, Executing agency, working region component, test cell 6, pipette tips frame 8, working plate 12 and stationary platen 13 form；Executing agency by X-axis slide unit 2, Y-axis slide unit 3, Z axis slide unit 4 and rifle axis slide unit 5 form；It is fixedly connected in the negative direction upper surface of stationary platen 13 There is switch board 1, X-axis slide unit 2 is fixed at the positive direction upper surface of stationary platen 13 and is fixedly connected with switch board 1；In work Make to be fixedly connected with working region component on platen 12, working region component include 8 blocks of packing Target Board 7,1 blocks of pipette tips plates, 1 piece 9,1 pieces of drain Target Boards 10 of stoste plate and 1 pipette tips separator 11, working plate 12 use the layout centered on stoste plate 9 Form, 8 pieces of packing Target Boards 7 are uniformly distributed in the both sides of stoste plate 9X axis directions, 4 pieces of packing Target Boards 7 of positive direction, negative direction 4 pieces of packing Target Boards 7, pipette tips frame 8 are arranged in the Y-axis positive direction of stoste plate 9, and drain Target Board 10 and pipette tips separator 11 divide It is not arranged in the Y-axis negative direction of stoste plate 9.This distribution form centered on stoste plate 9 can improve packing and drain Speed, shorten packing and drain time.

X-axis slide unit 2 is connect with Y-axis slide unit 3 using planer type structure, and the upper surface of X-axis slide unit 2 is fixedly installed X-axis and drags Plate is bolted two root posts on X-axis planker and holds up Y-axis slide unit 3, and Z axis slide unit 4 is lifted with Y-axis slide unit 3 using side Structure type connection, be fixedly installed Y-axis planker in the upper surface of Y-axis slide unit 3, be fixedly installed on Y-axis planker Z axis cunning Platform 4, rifle axis slide unit 5 are arranged on the side of Z axis slide unit 4.Thereby realize the linear motion of three degree of freedom.

Internal controller, DC power supply, driver and device driving motor, device driving electricity are provided in switch board 1 Machine driving X-axis slide plate, Y-axis slide plate, Z axis slide plate and rifle axis skateboarding.The surface of packing target version is provided with 12 × 5 holes position 14, the upper right corner of each hole position 14 is provided with the half elliptic step protrusion 15 for fixing centrifuge tube pipe lid.Test is single Member 6 is arranged on the upper surface of working plate 12 and in the Y-axis positive direction of stoste plate 9.

The host header piston rod unit of the utility model device is driven by a stepper motor, and silk is driven by stepper motor The convert rotational motion of bar is the straight reciprocating motion of piston rod, to realize that drawing liquid beats the function of liquid.The helical pitch of screw is 2mm, The straight reciprocating motion precision of piston rod is up to 0.1mm.Entire moving cell is made of three ball screw straight line slide units.It repeats Positioning accuracy is in 0.02mm hereinafter, work reliable and stable under can fully ensuring that the high-precision state of device.

The utility model dispenses and the structure of mixing arrangement is as shown in Figure 2.First, X-axis slide unit 2 uses dragon with Y-axis slide unit 3 Door type structure form, the structure type do not have fixed upper beam, can be greatly saved device space, facilitate upper liquid and take liquid work Make.Z axis slide unit 4, using the structure type of side lifting, facilitates the installation maintenance of Z axis slide unit 4 with Y-axis slide unit 3.Rifle axis slide unit 5 It is arranged on the side of Z axis slide unit 4, rifle axis slide unit 5 uses Open architecture, is convenient for changing the liquid getting device and its rifle of not co-content Head.Device uses two layers of platen form, and lower floor is the stationary platen 13 for installing X-axis slide unit 2, and pipette tips frame 8, stoste is are placed in upper strata The working plate 12 of plate 9, packing Target Board 7 and drain Target Board 10 etc., working plate 12 are fixed, the liquid of required operation Can remains stationary state, ensure liquid picks and places quality.

The utility model dispenses and the control system hardware of mixing arrangement is as shown in figure 3, control system hardware installation is filling It puts in switch board 1, mainly includes device internal controller, DC power supply, driver and device driving motor.Device internal control Device is attached by RJ45 network interfaces with computer, the packing drain data and instruction that reception computer transmission comes, in device Portion's control parses packing drain data and instruction, and be converted to actuator voltage drive signal, is carried out by driver After drive signal amplification, X-axis slide plate, Y-axis slide plate, Z axis slide plate and rifle axis skateboarding are driven by device driving motor.Pass through Pipette tips frame 8, stoste plate 9, the location data for dispensing Target Board 7 and drain Target Board 10 realize pipette tips frame 8, stoste plate 9, packing The target interpolation and movement of Target Board 7 and drain Target Board 10, driving pipette tips move to pipette tips frame 8, stoste plate 9, packing target The designated position of plate 7 and drain Target Board 10 carries out imbibition and tapping, so as to fulfill packing and the apparatus function of drain.

Executing agency is made of X-axis slide unit 2, Y-axis slide unit 3, Z axis slide unit 4 and rifle axis slide unit 5.The controlling party of each slide unit Formula is identical, and is all independent control unit.The control principle of executing agency is with operation logic：

1）Operator makes packing drain data file first, and data are saved as excel data files；

2）Running gear operates software, will dispense drain data import operation software, and clear and definite specified device is packing work Operation mode or drain operating mode；

3）Controller inside computer and device is connected by RJ45 network connections, device operation software is read automatically to be divided Fill drain data file；

4）Packing drain data conversion is instructed and is sent to inside device by procotol by device operation software in order to control Controller；

5）Control instruction is converted to X-axis, Y-axis, the moving interpolation voltage drive signals of Z axis by device internal controller, hair It send to driver；

6）Driver will voltage signal amplification and distribution after, be sent to X-axis, Y-axis, Z axis driving motor；

7）X-axis, Y-axis, the motor driving pipette tips of Z axis move to pipette tips frame 8, stoste plate 9, packing Target Board 7 and drain mesh The designated position of target 10；

8）The liquid of specified liquid measure is drawn and placed to rifle spindle motor according to liquid measure order-driven rifle axis skateboarding；

9）X-axis, Y-axis, Z axis and rifle axis motor receive halt instruction after, stop motor operation, complete packing drain work Make.

Fig. 4 is the control principle drawing of slide unit：Motor is linked by shaft coupling and screw, and planker is fixed on the nut on screw On.The rotary motion of oneself can be passed to planker by motor, it be made to be finally completed straight reciprocating motion, the slide unit in each direction Principle is identical.

As shown in Fig. 2, the present apparatus has been independently arranged calibrating position, a right hole is calibrating position pipette tips position, and interstitial hole is original Fluid apertures, the first from left hole are calibration target hole.In Calibration interface drop-down menu, calibrating position can be corrected, operated herein Person need to only calibrate pipette tips position.

As shown in figure 5, new design of the packing Target Board 7 using centrifuge tube frame plate, this centrifuge tube shelf are added by aluminum material Work forms, and 12 × 5 holes position 14 is contained on surface, and 14 upper right corner of each hole position are provided with raised half elliptic step, for solid Determine centrifuge tube pipe lid.

It dispenses or mixed process, the application method of the utility model device can be divided into packing/mixing demand ginseng Number obtains, the Extraction medium from specified pipe or plate（Primer or probe）, liquid relief and quantitatively to get medium etc. into specified pipe/plate several A step, the specific operation process of each step are：

Step 1：Packing or the acquisition of mixing demand parameter：

The purpose of this step is the data module of compatible universal, and the need for needing to dispense or mix are obtained in a manner of easily Parameter is sought, and is converted to the operation and control instruction of device.

1.1st, in the selection of computer control interface and DNA synthesizer on-line operation or off-line operation；

1.2nd, packing pattern or mixed mode are selected；

1.3rd, demand data, that is, the specific requirement for dispensing or mixing are read；

When 1.3.1, with DNA synthesizer on-line operation, in DNA synthesis digital management systems, according to the need of customer order It asks and has inputted, the utility model device is using digital module acquisition compatible and management system, it can be achieved that demand data Be introduced directly into；

When 1.3.2, with DNA synthesizer off-line operation, directly treat that operating file folder is established in the control computer of the present apparatus Packing or mixing demand information, including the position for intending dispensing or blending agent is initial（Hole, pipe）, volume, the position that will be transferred to It puts（Hole, pipe）Etc. information；

The 1.4th, combined operation that the demand parameter for dispensing or mixing is converted to each mechanism of device instructs.

Step 2：The Extraction medium from specified pipe/plate：

The purpose of this step is according to the demand parameter for intending packing or mixing, is joined by the three-dimensional of each motion It is dynamic, realize that fixed liquid relief pipette tips from specified plate hole or centrifuge tube, precisely extract the primer or probe of certain volume.

2.1st, the 1st step in Fig. 1：Host head unit links with the slipway mechanism of X-direction and Y-direction, is moved on pipette tips frame 8 Side；2nd step in Fig. 1：It is moved downward by host header Z axis, host header is made to fix a liquid transfer gun head, then rises to appropriate height Degree；3rd step in Fig. 1：The host head unit of pipette tips has been plugged, has been linked again with the slipway mechanism of X-direction and Y-direction, movement To the top of stoste plate 9 or pipe, by moving downward for Z axis, the liquid that pipette tips is made to be inserted into 9/ pipe corresponding hole site 14 of stoste plate In；4th step in Fig. 1：Unload used pipette tips.

2.2nd, the motor driving piston rod on host header, will be in the DNA liquid extractions sucking liquid transfer gun head of given dose.

2.3rd, host header Z axis moves upwards, and pipette tips is made to be detached from stoste, and is promoted to appropriate height so that motion process Middle pipette tips will not be in contact with the plate tube in operating table surface.

Step 3：Liquid relief simultaneously quantitatively gets medium into specified pipe/plate：

The main purpose of this step is that the DNA primer that will have been extracted or probe liquid are moved to the plate hole specified or centrifugation Pipe position, and realize precisely getting for medium requirements dosage.

3.1st, it is linked by the slipway mechanism of X-direction and Y-direction, the host header for being loaded with DNA liquid is moved to accordingly Above centrifuge tube or centrifugal hole.

3.2nd, it is moved downward by host header Z axis, liquid transfer gun head is inserted into corresponding plate hole or centrifuge tube.

3.3rd, the motor driving piston rod on host header, by the push of the DNA liquid of given dose get corresponding plate hole or In centrifuge tube.

3.4th, host header Z axis moves upwards, and is promoted to appropriate height, is ready for subsequent motion.

It, then need to be according to the phase of step 3.1 to 3.4 if necessary to which DNA primer or probe liquid are assigned in several pipe/holes Same principle, the DNA liquid liquid relief and distribution for completing each specified pipe/hole are got, so as to fulfill primary extracting, the mesh of porous distribution 's.

3.5th, as shown in Figure 1, host header links with the slipway mechanism of X-direction and Y-direction, it is moved to pipette tips separator 11 It puts, pipette tips separator 11 blocks pipette tips, and host header is moved upwards along Z axis.As shown in the 4th step in Fig. 1, used rifle is unloaded Head, each primer or probe extracting, liquid relief and get can only a disposable pipette tips, to avoid there is cross contamination.

3.6th, host header moves to initial position, completes the packing of a primer or probe or mixing liquid relief operation.

According to the requirement for intending packing or mixing, there is primer/probe in several source plate hole/pipes to need to dispense or mix, just Repeat above step two and step 3 several times.The present apparatus can be automatic to be connected according to the complete parameter obtained in step 1 All operationss are completed, centre is not paused, unless cause specific needs human intervention, can be suspended by button, then use When recovery button continue to run with.

Finally it should be noted that more than content is only to illustrate the technical solution of the utility model rather than to this practicality The limitation of novel protected range, simple modification that those of ordinary skill in the art carry out the technical solution of the utility model or Person's equivalent replacement, all without departing from the spirit and scope of technical solutions of the utility model.

Claims (5)

1. accurate packing and the mixing arrangement of a kind of DNA primer or probe, it is characterised in that：The accurate packing and mixing arrangement Mainly it is made of switch board, executing agency, working region component, test cell, pipette tips frame, working plate and stationary platen；Institute Executing agency is stated to be made of X-axis slide unit, Y-axis slide unit, Z axis slide unit and rifle axis slide unit；In the negative direction upper table of the stationary platen Face is fixedly connected with the switch board, the X-axis slide unit be fixed at the positive direction upper surface of the stationary platen and with institute Switch board is stated to be fixedly connected；The working region component, the working region component are fixedly connected on the working plate It is adopted including 8 pieces of packing Target Boards, 1 block of pipette tips plate, 1 block of stoste plate, 1 piece of drain Target Board and 1 pipette tips separator, working plate To the distribution form centered on stoste plate, 8 pieces of packing Target Boards are uniformly distributed in the both sides of stoste plate X-direction, positive direction 4 Block dispenses Target Board, and 4 pieces of packing Target Boards of negative direction, the pipette tips frame is arranged in the Y-axis positive direction of the stoste plate, described Drain Target Board and the pipette tips separator are respectively arranged in the Y-axis negative direction of the stoste plate.

2. accurate packing and the mixing arrangement of a kind of DNA primer according to claim 1 or probe, it is characterised in that：Institute It states X-axis slide unit to connect using planer type structure with Y-axis slide unit, the upper surface of X-axis slide unit is fixedly installed X-axis planker, drags in X-axis It is bolted two root posts on plate to hold up Y-axis slide unit, the Z axis slide unit uses the structure shape of side lifting with Y-axis slide unit Formula connects, and is fixedly installed Y-axis planker in the upper surface of Y-axis slide unit, the Z axis slide unit, institute are fixedly installed on Y-axis planker State the side that rifle axis slide unit is arranged on the Z axis slide unit.

3. accurate packing and the mixing arrangement of a kind of DNA primer according to claim 1 or 2 or probe, it is characterised in that： Internal controller, DC power supply, driver and device driving motor, described device driving motor are provided in the switch board Drive the X-axis slide plate, Y-axis slide plate, Z axis slide plate and rifle axis skateboarding.

4. accurate packing and the mixing arrangement of a kind of DNA primer according to claim 1 or 2 or probe, it is characterised in that： The surface of the packing target version is provided with 12 × 5 holes position, is provided in the upper right corner of each hole position for fixed centrifugation The half elliptic step protrusion of pipe pipe lid.

5. accurate packing and the mixing arrangement of a kind of DNA primer according to claim 1 or 2 or probe, it is characterised in that： The test cell is arranged on the upper surface of the working plate and in the Y-axis positive direction of the stoste plate.