CN107836545A - A kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof - Google Patents

A kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof Download PDF

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Publication number
CN107836545A
CN107836545A CN201711088894.4A CN201711088894A CN107836545A CN 107836545 A CN107836545 A CN 107836545A CN 201711088894 A CN201711088894 A CN 201711088894A CN 107836545 A CN107836545 A CN 107836545A
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CN
China
Prior art keywords
jerusalem artichoke
tea bag
tender shoots
life
nourishing tea
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CN201711088894.4A
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Chinese (zh)
Inventor
左兆河
张秀波
杨诚
杨慧敏
史建伟
刘兴岭
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Shenzhen Reed Health Technology Co Ltd
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Shenzhen Reed Health Technology Co Ltd
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Priority to CN201711088894.4A priority Critical patent/CN107836545A/en
Priority to PCT/CN2018/073554 priority patent/WO2019090975A1/en
Publication of CN107836545A publication Critical patent/CN107836545A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/34Tea substitutes, e.g. matè; Extracts or infusions thereof

Abstract

The present invention provides a kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof, and the jerusalem artichoke leaves life-nourishing tea bag is made up of the component of following mass fraction:10~20 parts of 50~100 parts of jerusalem artichoke tender shoots, 20~50 parts of jerusalem artichoke flower, 10~25 parts of grape pip and Mount Taishan Cordyceps militaris.Jerusalem artichoke is fermented in the jerusalem artichoke tea bag preparation process, it is set to carry out biological oxidation, a series of oxidizing process is carried out to catechin and Tea Polyphenols using the oxidases that blade itself contains, generate intermediate oxidation product theaflavin and thearubigin, to reduce the convergence of Tea Polyphenols and catechin, the stimulation to gastric mucosa is reduced, while there is the effect of reducing blood lipid, prevention cardiovascular and cerebrovascular disease.

Description

A kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof
Technical field
The present invention relates to food processing technology field, more particularly to a kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof.
Background technology
Jerusalem artichoke (Helianthus tuberosus L), also known as ground ginger, Jerusalem artichoke and Jerusalem artichoke, be composite family Helianthus for many years Raw herbaceous plant, originate in North America, the incoming Europe of 17th century, rear incoming China.Contain abundant nutrients in leaf of canada potato Matter, and contain natural biological antibiotic-chlorogenic acid, according to measurement, dry leaf of canada potato Content of Chlorogenic Acid is up to 3% or so.Green original Acid has extensive bioactivity, and research of the modern science to chlorogenic acid bioactivity has been deep into food, health care, medicine and day With multiple fields such as chemical industry.Chlorogenic acid is a kind of important bioactive substance, have antibacterial, it is antiviral, increase white blood cell, protect Liver cholagogic, antitumor, lowering blood pressure and blood fat, remove the effect such as free radical and stimulating central nervous system system.
How fully the Land use systems of jerusalem artichoke are relatively single at present, and the research that its blade utilizes also is rarely reported, profit With the abundant nutriment of leaf of canada potato and its health functions is developed, turns into and studies urgent problem from now on.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of by way of jerusalem artichoke tender shoots is fabricated into tea bag, carry Its high portability, while other beneficiating ingredients are added, drunk by way of nutriment is dissolved in into water by human body so as to be subject to Absorb, improve the utilization ratio of jerusalem artichoke leaves nutritional ingredient, reach health-care efficacy.
The present invention is achieved through the following technical solutions, there is provided a kind of jerusalem artichoke leaves life-nourishing tea bag, by following parts by weight Several components are made:
10~20 parts of 50~100 parts of jerusalem artichoke tender shoots, 10~25 parts of jerusalem artichoke flower, 10~25 parts of grape pip and Mount Taishan Cordyceps militaris.
The present invention also provides a kind of preparation method of jerusalem artichoke leaves life-nourishing tea bag, and methods described is carried out in accordance with the following steps:
S01:Ready fresh jerusalem artichoke flower is subjected to homogeneous, jerusalem artichoke homogenate is obtained after filtering, then by ready chrysanthemum Taro tender shoots stirs with jerusalem artichoke homogenate, kneads repeatedly to jerusalem artichoke leaves uniformly into bar.Preferably, ready fresh jerusalem artichoke flower Homogeneous is carried out using high pressure homogenizer.
S02:The obtained products of the step S01 are uniformly spread, is covered and is fermented with ventilative cotton.
Contain Tea Polyphenols and catechin in jerusalem artichoke tender shoots, Tea Polyphenols and catechin have convergence, and thorn is produced to gastric mucosa Swash, therefore, jerusalem artichoke tender shoots is underutilized always in diet domain for a long time.The present invention is by jerusalem artichoke tender shoots and jerusalem artichoke flower Mixed fermentation is homogenized, carries out biological oxidation, one system is carried out to catechin and Tea Polyphenols using the oxidases that blade itself contains The oxidizing process of row, intermediate oxidation product theaflavin and thearubigin are generated, to reduce the convergence of Tea Polyphenols and catechin, reduced Stimulation to gastric mucosa, meanwhile, make the product after fermentation that there is the effect of reducing blood lipid, prevention cardiovascular and cerebrovascular disease.Jerusalem artichoke tender shoots Mixing after fermentation is homogenized with jerusalem artichoke flower, can also be promoted main in main active chlorogenic acid and the jerusalem artichoke flower in jerusalem artichoke tender shoots The abundant fusion of active component flavonoids, while decompose tannin etc. in jerusalem artichoke flower using the polyphenol oxidase in jerusalem artichoke tender shoots and carry The material of bad smell, its main component is promoted to play synergistic function, increase product drinks mouthfeel.
S03:Product low-temperature passivation after the step S02 is fermented, then dries, dried product is pulverized End, obtain jerusalem artichoke tender shoots powder.
Preferably, the product after fermentation carries out low-temperature passivation and drying using vacuum freeze drier, is on the one hand passivation The activity of remaining polyphenol oxidase enzyme after fermentation, prevents it from continuing to aoxidize in process, influences finished product mouthfeel and nutriture value Value, on the other hand, it can continue to reduce the moisture in leaf of canada potato during vacuum refrigeration, extend its shelf-life.
S04:Sieved after ready grape pip and Mount Taishan Cordyceps militaris are ground into powder respectively, be then respectively placed in water Middle progress ultrasonic disruption extraction, dries after extract solution is concentrated, obtains auxiliary material powder.Preferably, grape pip and Mount Taishan pupa Cordyceps sinensis powder, which is respectively placed in water, is repeated ultrasonic disruption extraction, until residue is canescence, to improve recovery rate;It will carry Liquid is taken to be dried after concentrating by the way of spray drying.
Main component is respectively cyanine oligomer and cordycepin in auxiliary material powder, is water-soluble active ingredient, leaching Easy dissolution during bubble.Anthocyanidin oligomer has very strong radical scavenging activity and activity in vivo, has beautifying face and moistering lotion, changes Kind sleep and mitigation menopausal syndrome and other effects;Cordycepin belongs to ucleotides active material, have antitumor, anti-aging, Remove interior free yl and other effects.
S05:The auxiliary material powder that the obtained jerusalem artichoke tender shoots powder of the step S03 and the step S04 are obtained mixes equal It is even, it is fitted into non-woven fabrics tea bag, obtains jerusalem artichoke leaves life-nourishing tea bag.
Preferably, in the step S03, dried product is pulverized last, using air blast counter-current separation Method separates the blade epidermal hair in powder with blade powder, obtains jerusalem artichoke tender shoots powder.
Remove blade surface fine hair purpose be because of glandular secretion alkaloids substance in blade epidermal hair, as nicotine, Terpenoid etc., can reduce influence of such material to product quality after its epidermal hair is removed, acquisition preferably drinks mouthfeel.
Preferably, the jerusalem artichoke tender shoots is tender shoots at the top of the jerusalem artichoke plant for being in growth period.Jerusalem artichoke after harvesting is tender Bud cleaning, uniform sprinkling ascorbic acid solution after drying, sprinkling is kept in dark place standby after terminating.It is preferably, described anti-bad The concentration of hematic acid solution is 0.01~0.1%.Sprinkling ascorbic acid solution is to carry out color protection to jerusalem artichoke tender shoots.Except spraying Vitamin C Outside acid solution, other liquid can also be used to carry out color protection, such as water containing lemon juice etc..
Preferably, the jerusalem artichoke flower is:The jerusalem artichoke flower not yet to be bursted forth in bud stage.Various trace elements in bud Content is higher than in jerusalem artichoke flower in full bloom, and micronutrient levels is as follows in jerusalem artichoke bud:Mg:1462mg/kg, Ca:5621mg/kg, Fe:77mg/kg, Zn:16mg/kg, Mn:16mg/kg;Micronutrient levels is as follows in jerusalem artichoke flower in full bloom, Mg:709mg/kg, Ca:2218mg/kg, Fe:51mg/kg, Zn:10mg/kg, Mn:6.1mg/kg.Using jerusalem artichoke bud prepare jerusalem artichoke flower homogenate with The mixing of jerusalem artichoke tender shoots is fermented, and relative to jerusalem artichoke flower in full bloom, the trace element and active material in bud are in fermentation process In be easier to play a role, promote the synergistic function between each active component of jerusalem artichoke tender shoots, increase the edible mouth of product Sense and nutrition content.
Fake preferably, in the step S04, parameter is as follows when sonicator works, ultrasonic time:Interval time= 5s:5s, 30~45min of total duration, about 4 DEG C or so of ice bath temperature;Rotary Evaporators operating parameter is when extract solution concentrates:Rotating speed 100~150r/min, 40~50 DEG C of temperature, 30~45min of time.
Preferably, methods described also includes step S06:The step S05 tea bags dispensed are sterilized, gone out Room temperature is cooled to after the completion of bacterium, obtains jerusalem artichoke leaves life-nourishing tea bag finished product.
Preferably, the sterilizing is carried out in high-pressure steam sterilizing pan, sterilising temp is preferably 121 DEG C, and the time is 20min。
Preferably, in the step S01, fresh jerusalem artichoke flower carries out homogeneous using high pressure homogenizer, described high-pressure homogeneous The operating pressure of machine is 150~200bar, and 50~100 mesh sieves are crossed after homogeneous and obtain jerusalem artichoke homogenate;The jerusalem artichoke tender shoots and jerusalem artichoke Homogenate mixing, time of kneading is 40~90min.
Preferably, in the step S02, it is 10~20cm to spread thickness, and relative humidity is 60~80% in heap, fermentation Temperature is 25~35 DEG C, and fermentation time is 12~36h.
Fermentation condition is highly important to fermentation process, and the present invention is learnt by multiple Optimal Experimental, using above-mentioned hair When ferment condition is fermented, ferment effect is best, and obtained product is in eating mouth feel, nutrition content etc. Optimal.
Each step of jerusalem artichoke leaf teabag preparation method of the present invention complements each other, is indispensable, is one organically whole Body, for preparing raw material used in tea bag, the present invention from the materials (such as selection of jerusalem artichoke flower materials) of single raw material, from Manage (such as raw material is to add in what manner), how to be coordinated between different material, selection is optimized, final system It is standby to have obtained all good jerusalem artichoke leaves life-nourishing tea bag of flavor, mouthfeel, nutrition.
Technical scheme provided in an embodiment of the present invention can include following beneficial effect:
(1) jerusalem artichoke tender shoots, jerusalem artichoke flower, grape pip and Mount Taishan pupa have been merged in the jerusalem artichoke leaves life-nourishing tea bag that the present invention obtains The various active composition of cordyceps sinensis, each composition, which is respectively provided with, removes free radical, the function of anti-lipid peroxidation process, also, a variety of Antioxidation activity is higher than single constituent when combinations of substances uses.
(2) present invention gained jerusalem artichoke tea bag is obtained with zymotechnique, and catechin and Tea Polyphenols are aoxidizing in fermentation process Theaflavin and thearubigin are generated in the presence of enzyme, reduces the excitant to gastric mucosa, meanwhile, the product after fermentation has drop blood The effect of fat and prevention cardiovascular and cerebrovascular disease.
(3) leaf of canada potato epidermal hair is eliminated in the present invention, reduces in body of gland alkaloids substance to product quality Influence, can obtain and preferably drink mouthfeel.
(4) present invention with the addition of grape pip and the extract of Mount Taishan Cordyceps militaris, enrich active component in tea bag leachate Species and content, improve jerusalem artichoke leaves life-nourishing tea bag nutrition and healthcare function it is comprehensive so that gained jerusalem artichoke leaves are supported Raw tea bag is suitable for people of all ages.
Embodiment
In order that those skilled in the art more fully understand the technical scheme in the present invention, the present invention will be implemented below Technical scheme in example is clearly and completely described, it is clear that described embodiment is only that a present invention part is implemented Example, rather than whole embodiments.Based on the embodiment in the present invention, those skilled in the art are not making creative work Under the premise of the every other embodiment that is obtained, should all belong to protection scope of the present invention.
Embodiment 1
The present embodiment provides a kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof, the jerusalem artichoke leaves life-nourishing tea bag by with The component of lower parts by weight is made:
60 parts of jerusalem artichoke tender shoots, 25 parts of jerusalem artichoke flowers, 25 parts of grape pips and 15 parts of Mount Taishan Cordyceps militaris.
The jerusalem artichoke leaves life-nourishing tea bag of the present embodiment is prepared in accordance with the following steps:
S01:The top tender shoots for the jerusalem artichoke plant for being in growth period is chosen as raw material;After tender shoots is cleaned, drying, uniformly Sprinkling mass fraction be 0.05% ascorbic acid solution, carry out color protection, sprinkling terminate after be kept in dark place;Treat that jerusalem artichoke enters flower During flower bud phase, in 9 points to 10 points fine of the morning, it is acquired after the dewdrop evaporation drying on jerusalem artichoke flower, obtains jerusalem artichoke Flower raw material;Fresh jerusalem artichoke flower is placed in high pressure homogenizer and carries out homogeneous, homogenization pressure 150bar, chrysanthemum is obtained after crossing 60 mesh sieves Taro is homogenized;Then by the jerusalem artichoke tender shoots after color protection and jerusalem artichoke homogenate in mass ratio 1:After 0.3 ratio is mixed thoroughly, carry out repeatedly Knead until jerusalem artichoke leaves are uniformly into bar, time of kneading is about 60min.
S02:Jerusalem artichoke leaves bar after kneading uniformly is spread, and it is 15cm to spread thickness, and indoor temperature is 30 DEG C, relative in heap Humidity is 75%, is fermented after being covered with ventilative cotton to it, fermentation time 24h.
S03:Jerusalem artichoke tender shoots after fermentation is placed in freeze drier polyphenol oxidase activity in low-temperature passivation blade, very Reciprocal of duty cycle is 120mbar, while reduces in blade moisture to 13%;The jerusalem artichoke tender shoots obtained after drying is ground in grinding machine Clay into power shape, grind size is 60 mesh;Then using the method for air blast counter-current separation by blade epidermal hair and blade in powder Powder separates, and collection obtains jerusalem artichoke tender shoots powder.
S04:Sieved after grape pip and Mount Taishan Cordyceps militaris are ground into powder respectively, it is ultrasonic respectively at being carried out in water repeatedly The broken extraction of ripple, extraction total time are 30min, ultrasonic time:Interval time=5s:5s, by extract solution in Rotary Evaporators It is spray-dried after concentration, Rotary Evaporators rotating speed 110r/min, bath temperature is 40 DEG C, time 40min.
S05:The auxiliary material powder that the obtained jerusalem artichoke tender shoots powder of the step S04 and the step 05 are obtained is by above-mentioned matter Amount part fraction is well mixed, and is fitted into the specification of 2g/ bags in heat-sealing type non-woven fabrics tea bag, is obtained jerusalem artichoke leaves life-nourishing tea bag.
S06:The tea bag dispensed in the step S05 is sterilized in high-pressure steam sterilizing pan, sterilising temp For 121 DEG C, time 20min, room temperature is cooled to after the completion of sterilizing and obtains jerusalem artichoke leaves life-nourishing tea bag finished product.
Embodiment 2
The present embodiment provides a kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof, the jerusalem artichoke leaves life-nourishing tea bag by with The component of lower parts by weight is made:
100 parts of jerusalem artichoke tender shoots, 20 parts of jerusalem artichoke flowers, 15 parts of grape pips and 15 parts of Mount Taishan Cordyceps militaris.
The jerusalem artichoke leaves life-nourishing tea bag of the present embodiment is prepared in accordance with the following steps:
S01:The top tender shoots for the jerusalem artichoke plant for being in growth period is chosen as raw material;After tender shoots is cleaned, drying, uniformly Sprinkling mass fraction be 0.01% ascorbic acid solution, carry out color protection, sprinkling terminate after be kept in dark place;Treat that jerusalem artichoke enters flower During flower bud phase, in 9 points to 10 points fine of the morning, it is acquired after the dewdrop evaporation drying on jerusalem artichoke flower, obtains jerusalem artichoke Flower raw material;Fresh jerusalem artichoke flower is placed in high pressure homogenizer and carries out homogeneous, homogenization pressure 180bar, chrysanthemum is obtained after crossing 80 mesh sieves Taro is homogenized;Then by the jerusalem artichoke tender shoots after color protection and jerusalem artichoke homogenate in mass ratio 1:After 0.4 ratio is mixed thoroughly, carry out repeatedly Knead until jerusalem artichoke leaves are uniformly into bar, time of kneading is about 70min.
S02:Jerusalem artichoke leaves bar after kneading uniformly is spread, and it is 10cm to spread thickness, and indoor temperature is 28 DEG C, relative in heap Humidity is 70%, is fermented after being covered with ventilative cotton to it, fermentation time 12h.
S03:Jerusalem artichoke tender shoots after fermentation is placed in freeze drier polyphenol oxidase activity in low-temperature passivation blade, very Reciprocal of duty cycle is 150mbar, while reduces in blade moisture to 10%;The jerusalem artichoke tender shoots obtained after drying is ground in grinding machine Clay into power shape, grind size is 80 mesh;Then using the method for air blast counter-current separation by blade epidermal hair and blade in powder Powder separates, and collection obtains jerusalem artichoke tender shoots powder.
S04:Sieved after grape pip and Mount Taishan Cordyceps militaris are ground into powder respectively, it is ultrasonic respectively at being carried out in water repeatedly The broken extraction of ripple, extraction total time are 40min, ultrasonic time:Interval time=5s:5s, by extract solution in Rotary Evaporators It is spray-dried after concentration, Rotary Evaporators rotating speed 100r/min, bath temperature is 40 DEG C, time 45min.
S05:The auxiliary material powder that the obtained jerusalem artichoke tender shoots powder of the step S04 and the step 05 are obtained is by above-mentioned matter Amount part fraction is well mixed, and is fitted into the specification of 2g/ bags in heat-sealing type non-woven fabrics tea bag, is obtained jerusalem artichoke leaves life-nourishing tea bag.
S06:The tea bag dispensed in the step S05 is sterilized in high-pressure steam sterilizing pan, sterilising temp For 121 DEG C, time 20min, room temperature is cooled to after the completion of sterilizing and obtains jerusalem artichoke leaves life-nourishing tea bag finished product.
Embodiment 3
The present embodiment provides a kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof, the jerusalem artichoke leaves life-nourishing tea bag by with The component of lower parts by weight is made:
80 parts of jerusalem artichoke tender shoots, 25 parts of jerusalem artichoke flowers, 25 parts of grape pips and 15 parts of Mount Taishan Cordyceps militaris.
The jerusalem artichoke leaves life-nourishing tea bag of the present embodiment is prepared in accordance with the following steps:
S01:The top tender shoots for the jerusalem artichoke plant for being in growth period is chosen as raw material;After tender shoots is cleaned, drying, uniformly Sprinkling mass fraction be 0.01% ascorbic acid solution, carry out color protection, sprinkling terminate after be kept in dark place;Treat that jerusalem artichoke enters flower During flower bud phase, in 9 points to 10 points fine of the morning, it is acquired after the dewdrop evaporation drying on jerusalem artichoke flower, obtains jerusalem artichoke Flower raw material;Fresh jerusalem artichoke flower is placed in high pressure homogenizer and carries out homogeneous, homogenization pressure 200bar, is obtained after crossing 100 mesh sieves Jerusalem artichoke is homogenized;Then by the jerusalem artichoke tender shoots after color protection and jerusalem artichoke homogenate in mass ratio 1:After 0.5 ratio is mixed thoroughly, carry out anti- Knead again until jerusalem artichoke leaves are uniformly into bar, time of kneading is about 90min.
S02:Jerusalem artichoke leaves bar after kneading uniformly is spread, and it is 20cm to spread thickness, and indoor temperature is 35 DEG C, relative in heap Humidity is 80%, is fermented after being covered with ventilative cotton to it, fermentation time 36h.
S03:Jerusalem artichoke tender shoots after fermentation is placed in freeze drier polyphenol oxidase activity in low-temperature passivation blade, very Reciprocal of duty cycle is 150mbar, while reduces in blade moisture to 10%;The jerusalem artichoke tender shoots obtained after drying is ground in grinding machine Clay into power shape, grind size is 100 mesh;Then using the method for air blast counter-current separation by blade epidermal hair and blade in powder Powder separates, and collection obtains jerusalem artichoke tender shoots powder.
S04:Sieved after grape pip and Mount Taishan Cordyceps militaris are ground into powder respectively, it is ultrasonic respectively at being carried out in water repeatedly The broken extraction of ripple, extraction total time are 35min, ultrasonic time:Interval time=5s:5s, by extract solution in Rotary Evaporators It is spray-dried after concentration, Rotary Evaporators rotating speed 120r/min, bath temperature is 50 DEG C, time 30min.
S05:The auxiliary material powder that the obtained jerusalem artichoke tender shoots powder of the step S04 and the step 05 are obtained is by above-mentioned matter Amount part fraction is well mixed, and is fitted into the specification of 2g/ bags in heat-sealing type non-woven fabrics tea bag, is obtained jerusalem artichoke leaves life-nourishing tea bag.
S06:The tea bag dispensed in the step S05 is sterilized in high-pressure steam sterilizing pan, sterilising temp For 121 DEG C, time 20min, room temperature is cooled to after the completion of sterilizing and obtains jerusalem artichoke leaves life-nourishing tea bag finished product.
The present embodiment provides a kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof, the jerusalem artichoke leaves life-nourishing tea bag by with The component of lower parts by weight is made:
50 parts of jerusalem artichoke tender shoots, 25 parts of jerusalem artichoke flowers, 10 parts of grape pips and 20 parts of Mount Taishan Cordyceps militaris.
The jerusalem artichoke leaves life-nourishing tea bag of the present embodiment is prepared in accordance with the following steps:
S01:The top tender shoots for the jerusalem artichoke plant for being in growth period is chosen as raw material;After tender shoots is cleaned, drying, uniformly Sprinkling mass fraction be 0.05% ascorbic acid solution, carry out color protection, sprinkling terminate after be kept in dark place;Treat that jerusalem artichoke enters flower During flower bud phase, in 9 points to 10 points fine of the morning, it is acquired after the dewdrop evaporation drying on jerusalem artichoke flower, obtains jerusalem artichoke Flower raw material;Fresh jerusalem artichoke flower is placed in high pressure homogenizer and carries out homogeneous, homogenization pressure 150bar, chrysanthemum is obtained after crossing 50 mesh sieves Taro is homogenized;Then by the jerusalem artichoke tender shoots after color protection and jerusalem artichoke homogenate in mass ratio 1:After 0.1 ratio is mixed thoroughly, carry out repeatedly Knead until jerusalem artichoke leaves are uniformly into bar, time of kneading is about 40min.
S02:Jerusalem artichoke leaves bar after kneading uniformly is spread, and it is 15cm to spread thickness, and indoor temperature is 25 DEG C, relative in heap Humidity is 60%, is fermented after being covered with ventilative cotton to it, fermentation time 24h.
S03:Jerusalem artichoke tender shoots after fermentation is placed in freeze drier polyphenol oxidase activity in low-temperature passivation blade, very Reciprocal of duty cycle is 100mbar, while reduces in blade moisture to 15%;The jerusalem artichoke tender shoots obtained after drying is ground in grinding machine Clay into power shape, grind size is 50 mesh;Then using the method for air blast counter-current separation by blade epidermal hair and blade in powder Powder separates, and collection obtains jerusalem artichoke tender shoots powder.
S04:Sieved after grape pip and Mount Taishan Cordyceps militaris are ground into powder respectively, it is ultrasonic respectively at being carried out in water repeatedly The broken extraction of ripple, extraction total time are 45min, ultrasonic time:Interval time=5s:5s, by extract solution in Rotary Evaporators It is spray-dried after concentration, Rotary Evaporators rotating speed 130r/min, bath temperature is 40 DEG C, time 45min..
S05:By the jerusalem artichoke tender shoots powder that the step 04 obtains and the auxiliary material powder that the step 05 obtains by above-mentioned matter Amount part fraction is well mixed, and is fitted into the specification of 2g/ bags in heat-sealing type non-woven fabrics tea bag, is obtained jerusalem artichoke leaves life-nourishing tea bag.
S06:The tea bag dispensed in the step S05 is sterilized in high-pressure steam sterilizing pan, sterilising temp For 121 DEG C, time 20min, room temperature is cooled to after the completion of sterilizing and obtains jerusalem artichoke leaves life-nourishing tea bag finished product.
Embodiment 5
The present embodiment provides a kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof, the jerusalem artichoke leaves life-nourishing tea bag by with The component of lower parts by weight is made:
70 parts of jerusalem artichoke tender shoots, 15 parts of jerusalem artichoke flowers, 20 parts of grape pips and 10 parts of Mount Taishan Cordyceps militaris.
The jerusalem artichoke leaves life-nourishing tea bag of the present embodiment is prepared in accordance with the following steps:
S01:The top tender shoots for the jerusalem artichoke plant for being in growth period is chosen as raw material;After tender shoots is cleaned, drying, uniformly Sprinkling mass fraction be 0.05% ascorbic acid solution, carry out color protection, sprinkling terminate after be kept in dark place;Treat that jerusalem artichoke enters flower During 20 days phases, in 9 points to 10 points fine of the morning, it is acquired after the dewdrop evaporation drying on jerusalem artichoke flower, obtains chrysanthemum Taro raw material;Fresh jerusalem artichoke flower is placed in high pressure homogenizer and carries out homogeneous, homogenization pressure 150bar, is obtained after crossing 60 mesh sieves Jerusalem artichoke is homogenized;Then by the jerusalem artichoke tender shoots after color protection and jerusalem artichoke homogenate in mass ratio 1:After 0.3 ratio is mixed thoroughly, carry out anti- Knead again until jerusalem artichoke leaves are uniformly into bar, time of kneading is about 60min.
S02:Jerusalem artichoke leaves bar after kneading uniformly is spread, and it is 15cm to spread thickness, and indoor temperature is 30 DEG C, relative in heap Humidity is 75%, is fermented after being covered with ventilative cotton to it, fermentation time 24h.
S03:Jerusalem artichoke tender shoots after fermentation is placed in freeze drier polyphenol oxidase activity in low-temperature passivation blade, very Reciprocal of duty cycle is 120mbar, while reduces in blade moisture to 13%;The jerusalem artichoke tender shoots obtained after drying is ground in grinding machine Clay into power shape, grind size is 60 mesh;Then using the method for air blast counter-current separation by blade epidermal hair and blade in powder Powder separates, and collection obtains jerusalem artichoke tender shoots powder.
S04:Sieved after grape pip and Mount Taishan Cordyceps militaris are ground into powder respectively, it is ultrasonic respectively at being carried out in water repeatedly The broken extraction of ripple, extraction total time are 45min, ultrasonic time:Interval time=5s:5s, by extract solution in Rotary Evaporators It is spray-dried after concentration, Rotary Evaporators rotating speed 150r/min, bath temperature is 45 DEG C, time 40min..
S05:By the jerusalem artichoke tender shoots powder that the step 04 obtains and the auxiliary material powder that the step 05 obtains by above-mentioned matter Amount part fraction is well mixed, and is fitted into the specification of 2g/ bags in heat-sealing type non-woven fabrics tea bag, is obtained jerusalem artichoke leaves life-nourishing tea bag.
S06:The tea bag dispensed in the step S05 is sterilized in high-pressure steam sterilizing pan, sterilising temp For 121 DEG C, time 20min, room temperature is cooled to after the completion of sterilizing and obtains jerusalem artichoke leaves life-nourishing tea bag finished product.
Comparative example 1
The present embodiment differs only in embodiment 5, without step S02 fermentation process, after directly kneading Jerusalem artichoke leaves bar is placed in freeze drier, carry out step S03 the step of, except without fermentation in addition to, other conditions and embodiment 5 are complete Exactly the same, here is omitted.
Comparative example 2
The present embodiment differs only in embodiment 5, uses jerusalem artichoke flower in full bloom to prepare jerusalem artichoke for raw material in step S01 Flower homogenate, in addition other conditions are identical with embodiment 5, and here is omitted.
To further illustrate the application value of jerusalem artichoke leaves life-nourishing tea bag provided in an embodiment of the present invention, inventor carries out Sensory evaluation, physical and chemical index analysis, external Scavenging ability and animal experiment, it tests process and result is as follows:
1st, jerusalem artichoke leaves life-nourishing tea bag sensory test
Take the jerusalem artichoke leaves life-nourishing tea bag that the embodiment of the present invention 1~5 obtains green as test group, commercially available Lipton pleasure series living Tea is control group 1, and seven bright cloud south tea bags are control group 2, according to tealeaves sensory method (GB/T 23776-2009, GB/T 14487), evaluated by 30 reviewers with qualification of sampling tea, provide the description of tea sample feature and score, full marks 100 divide, Results averaged, fragrance of the evaluation item including profile and endoplasm, soup look, flavour etc., term is evaluated with reference to tealeaves sensory Term GB/T14487-2008.
Sensory evaluation analysis is carried out in terms of millet paste color and luster, fragrance and flavour three, evaluation criterion is as shown in table 1.
The tea bag sensory evaluation standard of table 1
Sensory evaluation is carried out to the tea bag of test group and control group according to the standard of table 1, as a result as shown in table 2.
The Analyses Methods for Sensory Evaluation Results of table 2
Wherein, comparative example 3 is commercially available Lipton pleasure serial green tea living;Comparative example 4 is seven bright cloud south tea bags.
The Analyses Methods for Sensory Evaluation Results of above-mentioned table 2 shows that the jerusalem artichoke leaves life-nourishing tea bag that the embodiment of the present invention obtains is in soup look, taste Commercially available tea bag is superior in terms of taste and fragrance, there is larger market value.
2nd, jerusalem artichoke leaves life-nourishing tea bag physical and chemical index is analyzed
For the nutritive value of jerusalem artichoke leaves life-nourishing tea bag clearly provided in an embodiment of the present invention, it is real that the present invention is chosen respectively Apply tea bag that example obtains and commercially available Lipton pleasure serial green tea living, seven bright cloud south tea bags brew 5min in 100mL boiling water, take Millet paste determines water extraction (GB/T 8305), total amino acid content (GB/T 8314-2002), moisture (GB/T respectively And chlorogenic acid content (GB/T 22250-2008) 8304).Assay is as shown in table 3.
The jerusalem artichoke leaves life-nourishing tea bag physical and chemical index of table 3
In above-mentioned table 3, comparative example 3 is commercially available Lipton pleasure serial green tea living;Comparative example 4 is seven bright cloud south tea bags.By upper State table 3 and understand that the chlorogenic acid and amino acid content of jerusalem artichoke leaves life-nourishing tea bag provided in an embodiment of the present invention are above existing city Sell tea bag.
3rd, jerusalem artichoke leaves life-nourishing tea bag animal experiment
3.1 jerusalem artichoke leaves life-nourishing tea bag hypotensives are tested
Contain jerusalem artichoke leaves, jerusalem artichoke flower, grape pip and Mount Taishan pupa in jerusalem artichoke leaves life-nourishing tea bag provided in an embodiment of the present invention The compositions such as cordyceps sinensis, containing abundant chlorogenic acid and OPC isoreactivity material, so it has well in terms of controlling blood pressure Effect.In order to further illustrate the effect of present invention control blood pressure, healthy rat 180 is chosen in this experiment, male and female dual-purpose, with Machine is divided into 9 groups, wherein 8 groups are periodically subcutaneously injected desoxycortone 50mg/kg daily, continuous three weeks, induced rat produced high blood Symptom is pressed, the 9th group is tested as blank control group.The jerusalem artichoke that first five set feeding embodiment of the present invention 1 is prepared to embodiment 5 Leaf life-nourishing tea bag water, the 6th group and the 7th group feeds jerusalem artichoke leaves bag bubble prepared by comparative example 1 and comparative example 2 of the present invention respectively Tea, the 8th group is hypertension control group, refers to after induction produces stable hypertension and feeds normal water;7th group of blank control group is not Induction produces hypertension symptom and normal water.The blood pressure level of periodic detection rat, as a result as shown in table 4.
The rat blood pressure value of table 4 tests table
3.2 jerusalem artichoke leaves life-nourishing tea bag antioxidation activities are tested
The embodiment of the present invention prepare jerusalem artichoke leaves life-nourishing tea bag composition in containing chlorogenic acid, flavonoids, OPC and Cordycepic acid isoreactivity composition, mentioned component have the ability of stronger removing free radical, and this experiment is with experiment in vitro and animal reality The mode for testing combination removes free radical to it and antioxidation activity is tested, and concrete mode is as follows:
In vitro test:1. take the one or more in the extract solution of jerusalem artichoke tender shoots, jerusalem artichoke flower, grape pip and Mount Taishan Cordyceps militaris Combination carry out antioxidation in vitro experiment, calculate its hydroxy radical (OH) clearance rate, lipid oxidation inhibiting rate and DPPH are removed Rate, as a result as shown in table 5.
Wherein, hydroxy radical (OH) clearance rate is calculated using ferrous sulfate-hydrogen peroxide determination method;Lipid oxidation presses down Rate processed and DPPH clearance rates are measured using colorimetric method.
The external antioxidation activity table of jerusalem artichoke leaves life-nourishing tea bag active component of table 5
As shown in Table 5, the present invention takes contained active material in jerusalem artichoke tender shoots, jerusalem artichoke flower, grape pip and Mount Taishan Cordyceps militaris It is respectively provided with and removes free radical, the function of anti-lipid peroxidation process, wherein several antioxidation activities when being applied in combination is higher than single One constituent, illustrate that each active component quality inspection has a synergistic function, it is anti-oxidant caused by each active component interaction Ability is noticeably greater than single component.
2. take respectively the jerusalem artichoke leaves life-nourishing tea bag that is obtained in the embodiment of the present invention 1~5 and comparative example 1 and comparative example 2 in 10min is soaked in 100mL, 80 DEG C of hot water, takes its millet paste to carry out hydroxy radical (OH) clearance rate, lipid oxidation suppression respectively The experiment of rate and DPPH clearance rates, for test method with 1., result of the test is as shown in table 6.
Wherein, comparative example 3 is commercially available Lipton pleasure serial green tea living;Comparative example 4 is seven bright cloud south tea bags
The jerusalem artichoke leaves life-nourishing tea bag antioxidation activity in vitro table of table 6
As shown in Table 6, the jerusalem artichoke leaves life-nourishing tea bag that various embodiments of the present invention obtain is respectively provided with stronger removing free radical And suppressing the effect of lipid oxidation, its action effect is significantly higher than commercially available tea bag in comparative example.
Zoopery:The rat 200 of health is chosen, male and female dual-purpose is randomly divided into 10 groups, every group 20, first five component The jerusalem artichoke leaves life-nourishing tea bag millet paste and chow diet obtained in other feeding embodiment 1~5, the 6th group is blank control group, is fed Food normal water, the 7th group and the 8th group millet paste for feeding the tea bag that comparative example 1 and comparative example 2 of the present invention obtain respectively, the Nine groups and the tenth group feed commercially available Lipton pleasure serial green tea living and seven bright cloud south tea bags respectively.10 are measured respectively after feeding 30d Superoxide dismutase (SOD) activity, the change of MDA value (MDA), TAC (T-AOC) in group rat blood serum, Experimental result is as shown in table 7.
Note:Superoxide dismutase (SOD) activity uses nitroblue tetrazolium (NBT) photochemical reduction colorimetric method for determining;MDA Value (MDA) uses 2- thiobarbituricacidα-s (TBA) colorimetric method for determining;TAC (T-AOC) is determined using FRAP methods.
The rat blood serum oxidation activity index of table 7 changes
As shown in Table 7, the jerusalem artichoke leaves life-nourishing tea bag that various embodiments of the present invention obtain is in increase rat blood serum SOD and T- AOC activity aspect significant effects, while also there is stronger reduction MDA values.
It should be understood by a person of ordinary skill in the art that the discussion of any of the above embodiment is exemplary only, not It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples;Under the thinking of the present invention, above example Or can also be combined between the technical characteristic in different embodiments, step can be realized with random order, and exist such as Many other changes of upper described different aspect of the invention, for simplicity, they are not provided in details.Therefore, it is all Within the spirit and principles in the present invention, any omission for being made, modification, equivalent substitution, improvement etc., it should be included in the present invention's Within protection domain.
Certainly, described above is also not limited to the example above, the technical characteristic of the invention without description can by or Realized, will not be repeated here using prior art;It is not to this that above example, which is merely to illustrate technical scheme, The limitation of invention, the present invention is described in detail with reference to preferred embodiment, one of ordinary skill in the art should Understand, the variations, modifications, additions or substitutions that those skilled in the art are made in the essential scope of the present invention Without departure from spirit of the invention, it should also belong to claims of the invention.

Claims (10)

1. a kind of jerusalem artichoke leaves life-nourishing tea bag, it is characterised in that be made up of the component of following parts by weight:
10~20 parts of 50~100 parts of jerusalem artichoke tender shoots, 10~25 parts of jerusalem artichoke flower, 10~25 parts of grape pip and Mount Taishan Cordyceps militaris.
2. a kind of preparation method of jerusalem artichoke leaves life-nourishing tea bag, it is characterised in that methods described is carried out in accordance with the following steps:
S01:Ready fresh jerusalem artichoke flower is subjected to homogeneous, jerusalem artichoke flower homogenate is obtained after filtering, then by ready jerusalem artichoke Tender shoots stirs with jerusalem artichoke homogenate, kneads repeatedly to jerusalem artichoke leaves uniformly into bar;
S02:The obtained products of the step S01 are uniformly spread, is covered and is fermented with ventilative cotton;
S03:Product cryogenic vacuum after the step S02 is fermented is passivated, and then dries, dried product is pulverized End, obtain jerusalem artichoke tender shoots powder;
S04:Sieved after ready grape pip and Mount Taishan Cordyceps militaris are ground into powder respectively, then be respectively placed in water Row ultrasonic disruption extracts, and is dried after extract solution is concentrated, obtains auxiliary material powder;
S05:The obtained auxiliary material powder of the obtained jerusalem artichoke tender shoots powder of the step S03 and the step S04 is well mixed, filled Enter in non-woven fabrics tea bag, obtain jerusalem artichoke leaves life-nourishing tea bag.
A kind of 3. preparation method of jerusalem artichoke leaves life-nourishing tea bag according to claim 2, it is characterised in that the step In S03, dried product is pulverized last, using air blast counter-current separation method by the blade epidermal hair in powder with Blade powder separates, and obtains jerusalem artichoke tender shoots powder.
A kind of 4. preparation method of jerusalem artichoke leaves life-nourishing tea bag according to claim 2, it is characterised in that the step In S01, ready jerusalem artichoke tender shoots is:Cleaning after tender shoots harvesting will be at the top of the jerusalem artichoke plant in growth period, dries, is uniform Ascorbic acid solution is sprayed, sprinkling is kept in dark place to obtain after terminating.
A kind of 5. preparation method of jerusalem artichoke leaves life-nourishing tea bag according to claim 4, it is characterised in that the ascorbic acid The concentration of solution is 0.01~0.1%.
A kind of 6. preparation method of jerusalem artichoke leaves life-nourishing tea bag according to claim 2, it is characterised in that the jerusalem artichoke flower For the jerusalem artichoke flower of bud stage harvesting.
7. the preparation method of a kind of jerusalem artichoke leaves life-nourishing tea bag according to claim 2, it is characterised in that methods described is also Including step S06:
The step S05 tea bags dispensed are sterilized, room temperature is cooled to after the completion of sterilizing, obtain jerusalem artichoke leaves health bag Make tea finished product.
A kind of 8. preparation method of jerusalem artichoke leaves life-nourishing tea bag according to claim 2, it is characterised in that the step In S01, the fresh jerusalem artichoke flower carries out homogeneous using high pressure homogenizer, the operating pressure of the high pressure homogenizer for 150~ 200bar, 50~100 mesh sieves are crossed after homogeneous and obtain jerusalem artichoke homogenate;The jerusalem artichoke tender shoots mixes with jerusalem artichoke flower homogenate, time of kneading For 40~90min.
A kind of 9. preparation method of jerusalem artichoke leaves life-nourishing tea bag according to claim 2, it is characterised in that the step In S02, it is 10~20cm to spread thickness, and relative humidity is 60~80% in heap, and fermentation temperature is 25~35 DEG C, and fermentation time is 12~36h.
A kind of 10. preparation method of jerusalem artichoke leaves life-nourishing tea bag according to claim 2, it is characterised in that the step In S04, parameter is as follows when sonicator works, ultrasonic time:Interval time=5s:5s, 30~45min of total duration, ice Bath temperature is 4 DEG C;Extract solution uses Rotary Evaporators when concentrating, and Rotary Evaporators operating parameter is:100~150r/ of rotating speed Min, 40~50 DEG C of temperature, 30~45min of time.
CN201711088894.4A 2017-11-08 2017-11-08 A kind of jerusalem artichoke leaves life-nourishing tea bag and preparation method thereof Pending CN107836545A (en)

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PCT/CN2018/073554 WO2019090975A1 (en) 2017-11-08 2018-01-20 Helianthus tuberosus leaf health-preserving tea bag and preparation method therefor

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104054874A (en) * 2014-05-29 2014-09-24 邹峰 Instant grape-seed coffee powder and preparation method thereof
CN104256017A (en) * 2014-10-21 2015-01-07 叶青芝 Healthcare mulberry leaf and apple peel tea and preparation method thereof
CN105724693A (en) * 2016-03-03 2016-07-06 山东沾化亿元宝农业种植有限公司 Jerusalem artichoke tea and making method thereof
CN105941761A (en) * 2016-06-17 2016-09-21 江苏省农业科学院 Mixed culture fermentation tea bag and preparation method thereof
CN106173048A (en) * 2016-07-01 2016-12-07 廊坊市思科农业技术有限公司 Jerusalem artichoke scented tea and preparation method thereof
CN106615431A (en) * 2016-12-08 2017-05-10 山东新糖源农业科技有限公司 Sliced helianthus tuberosus tea and making method thereof
CN106798061A (en) * 2016-12-21 2017-06-06 山东新糖源农业科技有限公司 Jerusalem artichoke leaf tea and preparation method thereof
CN106900942A (en) * 2017-01-20 2017-06-30 陆川县米场镇合美种养专业合作社联合社 A kind of Mulberry-leaf Tea and preparation method thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104054874A (en) * 2014-05-29 2014-09-24 邹峰 Instant grape-seed coffee powder and preparation method thereof
CN104256017A (en) * 2014-10-21 2015-01-07 叶青芝 Healthcare mulberry leaf and apple peel tea and preparation method thereof
CN105724693A (en) * 2016-03-03 2016-07-06 山东沾化亿元宝农业种植有限公司 Jerusalem artichoke tea and making method thereof
CN105941761A (en) * 2016-06-17 2016-09-21 江苏省农业科学院 Mixed culture fermentation tea bag and preparation method thereof
CN106173048A (en) * 2016-07-01 2016-12-07 廊坊市思科农业技术有限公司 Jerusalem artichoke scented tea and preparation method thereof
CN106615431A (en) * 2016-12-08 2017-05-10 山东新糖源农业科技有限公司 Sliced helianthus tuberosus tea and making method thereof
CN106798061A (en) * 2016-12-21 2017-06-06 山东新糖源农业科技有限公司 Jerusalem artichoke leaf tea and preparation method thereof
CN106900942A (en) * 2017-01-20 2017-06-30 陆川县米场镇合美种养专业合作社联合社 A kind of Mulberry-leaf Tea and preparation method thereof

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Application publication date: 20180327