CN107827613A - A kind of microbial-bacterial fertilizer for promoting cucumber to take root and preparation method thereof - Google Patents
A kind of microbial-bacterial fertilizer for promoting cucumber to take root and preparation method thereof Download PDFInfo
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- CN107827613A CN107827613A CN201711339088.XA CN201711339088A CN107827613A CN 107827613 A CN107827613 A CN 107827613A CN 201711339088 A CN201711339088 A CN 201711339088A CN 107827613 A CN107827613 A CN 107827613A
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- cucumber
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- bacterial fertilizer
- bacterium solution
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 45
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 title claims abstract description 39
- 230000001737 promoting effect Effects 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 244000299906 Cucumis sativus var. sativus Species 0.000 title 1
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 43
- 239000000203 mixture Substances 0.000 claims abstract description 32
- 241000589156 Agrobacterium rhizogenes Species 0.000 claims abstract description 29
- 239000002028 Biomass Substances 0.000 claims abstract description 23
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 23
- 239000005416 organic matter Substances 0.000 claims abstract description 20
- 241000589158 Agrobacterium Species 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 235000009973 maize Nutrition 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 5
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims description 3
- 241000219793 Trifolium Species 0.000 claims description 3
- 239000002154 agricultural waste Substances 0.000 claims description 3
- 239000010828 animal waste Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000010902 straw Substances 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims description 2
- 241001037822 Bacillus bacterium Species 0.000 claims 2
- 238000003763 carbonization Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 15
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 235000015816 nutrient absorption Nutrition 0.000 abstract description 4
- 230000002786 root growth Effects 0.000 abstract description 4
- 235000013399 edible fruits Nutrition 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 230000008635 plant growth Effects 0.000 abstract 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 10
- 239000002689 soil Substances 0.000 description 5
- 210000003608 fece Anatomy 0.000 description 4
- 239000010871 livestock manure Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000004913 activation Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000008092 positive effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000305550 Streptopus amplexifolius Species 0.000 description 1
- 235000001231 Streptopus amplexifolius Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000008638 plant developmental process Effects 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 239000004016 soil organic matter Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to a kind of microbial-bacterial fertilizer for promoting cucumber to take root and preparation method thereof, the microbial-bacterial fertilizer is using the one or more in Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 as made of primary raw material.Agrobacterium rhizogenes (Agrobacterium rhizogenes) has the function that to promote plant establishment, and then the nutrient absorption efficiency to improving plant has important influence.Therefore, OD values are used to be mixed and made into microbial-bacterial fertilizer with organic matter composition and biomass carbon for 0.4 0.6 agrobacterium rhizogenes bacterium solution, topdress tri-leaf period cucumber seedling, because biological bacteria and cucumber form a kind of symbiosis, stimulate the root growth of cucumber and increase the absorption area of root, improve the nutrient absorption efficiency of cucumber, promote Plant Growth of Cucumber, overcome that fruit quality in cucumber production is low, the problem of yielding poorly, it agriculturally can be used for improving the quality of cucumber and increasing its yield, there is huge economic benefit.
Description
Technical field
The present invention relates to a kind of microbial-bacterial fertilizer for promoting cucumber to take root and preparation method thereof, belong to agricultural technology field.
Background technology
Cucumber (Cucumis sativus L.) is commonly encountered that the rate of rooting is low, and survival rate is low during growth, root
The problems such as system is fragile, and plant strain growth lacks nutrient, such as cucumber cucumber in growth course usually can in growing
The discoloration of generation blade, leaf roll, or even the physiological phenomenon such as withered, these problems directly results in the final growth quality of cucumber and production
The problem of amount reduces, in order to improve the growth quality of crop, increase crop yield, in 20 end of the centurys, the development of microbial-bacterial fertilizer should
Transport and give birth to.
Microbial-bacterial fertilizer is according to soil micro-ecosystem principle, Plant Nutrition principle and modern times " organic agriculture "
Basic conception and develop.Microbial manure is to cause crop to obtain required nutrient with the vital movement of active microorganism
A kind of new-type fertilizer biological products.Microbial-bacterial fertilizer, which contains up to more than ten, plants high-efficiency activated beneficial microbe bacterium, suitable for various
Crop uses, and can activate nutrient, improves nutrient utilization rate, has universal, has broken " selectivity " " limitation of common bio-fertilizer
The intrinsic weakness of property " " special fertilizer ", this is that other bio-feritlizers are incomparable.Microbial-bacterial fertilizer is the new of 21st century
Fertilizer, it is a kind of low charcoal, pure natural, nontoxic, harmless, free of contamination biological organic fertilizer, can increase soil fertility, improve soil
Character is activated, is kept soil from packing together；Soil fertilizer, water conservation are improved, cold tolerance, strengthens crop disease-resistant ability, is increased in soil
Organic matter, prevent pathogen invade, reduce plant pest and disease damage growth.
Agrobacterium rhizogenes, which infects rear plant, can produce many hairy roots, consolidate root system of plant, promote growth and development of plants, because
This prepares microbial-bacterial fertilizer using agrobacterium rhizogenes bacterium solution, and growing for plant is had great significance, raw in agricultural
In production, there is potential economic benefit.
The content of the invention
For above-mentioned phenomenon, it is an object of the invention to provide a kind of microbial-bacterial fertilizer for promoting cucumber to take root, micro- life
Thing bacterial manure solves cucumber in life using the distinctive effect for promoting plant root growth and strengthening rootability of agrobacterium rhizogenes
In growth process due to survival rate is low, fruit quality is low caused by root system is undeveloped, yield poorly the problem of, present invention also offers
A kind of preparation method for the microbial-bacterial fertilizer for promoting cucumber to take root.
The present invention is realized by following technical scheme：
A kind of microbial-bacterial fertilizer for promoting cucumber to take root, the raw material system that described microbial-bacterial fertilizer is matched by following component
Into：
Agrobacterium rhizogenes bacterium solution 15ml
Organic matter composition 400g
Biomass carbon 100g
Strain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more in 15834, strain derive from Chinese microorganism strain collection；
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind in animal wastes
It is or several；
The biomass carbon refers to that timber, grass, maize straw or other agricultural waste materials pass through height under low-oxygen environment
Anneal crack solution is carbonized the material to be formed.
Described agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
This method comprises the following steps：
1) preparation of agrobacterium rhizogenes bacterium solution, by Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
One or more of activation to OD values be 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.
2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon
4:1 is well mixed, obtains the mixture of organic matter composition and biomass carbon.
3) hair that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2)
Root Agrobacterium bacterium solution uniform stirring, the mixture of every 500g organic matters composition and biomass carbon add bacterium solution 15ml, obtain micro- life
Thing bacterial manure.
The present invention has following positive effect：
1st, the microorganism fungus kind that microbial-bacterial fertilizer of the present invention uses for agrobacterium rhizogenes strain A4, R1205, R1000,
One or more in R1601, R1022,15834, agrobacterium rhizogenes can induce cucumber and produce hairy root, promote cucumber root
Growth, play the role of to the nutrient absorption efficiency for improving cucumber important.
2nd, microbial-bacterial fertilizer of the present invention uses agrobacterium rhizogenes bacterium solution, and organic matter composition and biomass carbon are former material
Material, there is the advantages of cost is relatively low, easy to obtain, preparation method is simple, be readily put into production.
3rd, the preparation method for the microbial-bacterial fertilizer that a kind of promotion cucumber of the invention takes root is applied to the production of cucumber, solves
Plant in growth course due to root system it is undeveloped caused by survival rate is low, fruit quality is low, yield poorly the problem of, have huge
Big economic benefit.
Brief description of the drawings
Fig. 1 is the cucumber plant root system life that 15d is grown after applying microbial-bacterial fertilizer of the present invention based on agrobacterium rhizogenes
Long condition diagram.
Fig. 2 grows 15d cucumber plant root growth condition diagram not apply microbial-bacterial fertilizer of the present invention.
Embodiment
With reference to case study on implementation, the invention will be further described：
A kind of microbial-bacterial fertilizer for promoting cucumber to take root, the raw material system that described microbial-bacterial fertilizer is matched by following component
Into：
Agrobacterium rhizogenes bacterium solution 15ml
Organic matter composition 400g
Biomass carbon 100g
Strain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more in 15834, strain derive from Chinese microorganism strain collection；
The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind in animal wastes
It is or several；
The biomass carbon refers to that timber, grass, maize straw or other agricultural waste materials pass through height under low-oxygen environment
Anneal crack solution is carbonized the material to be formed.
Described agrobacterium rhizogenes bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
A kind of preparation method for the microbial-bacterial fertilizer for promoting cucumber to take root, this method comprise the following steps：
1) preparation of agrobacterium rhizogenes bacterium solution, by Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834
One or more of activation to OD values be 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.
2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon
4:1 is well mixed, obtains the mixture of organic matter composition and biomass carbon.
3) hair that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2)
Root Agrobacterium bacterium solution uniform stirring, the mixture of every 500g organic matters composition and biomass carbon add bacterium solution 15ml, obtain micro- life
Thing bacterial manure.
Effective viable bacteria >=3 × 10 of described microbial-bacterial fertilizer8cuf/g。
The application process for promoting cucumber to take root
Tri-leaf period cucumber seedling is planted, fertilizer is uniformly distributed in by soil plough horizon using whole layer placement of fertilizer method, about
500g/m2, per 15-20 days, fertilising once, had obvious well developed root system and eugonic spy in cucumber plant developmental process
Point, substantially increase the quality and yield of cucumber.
Microbial-bacterial fertilizer based on agrobacterium rhizogenes is in cucumber production application
In 60 plants of cucumber seedling for buying tri-leaf period on the market, every 30 plants are one group of plantation, are divided into control group and experimental group,
Control group is not applied fertilizer, and experimental group fertilising, is about applied fertilizer 500g using every ten plants of seedling of whole layer placement of fertilizer method, and fertilising in every 15 days once, is applied
Fertilizer 3 times, observes and records Reducing sugar.It is as shown in the table to record result：
The cucumber plant growing state of different number of days
As a result show, the cucumber for the microbial-bacterial fertilizer being applied with based on agrobacterium rhizogenes has substantially in developmental process
Survival rate it is high, well developed root system, eugonic feature, the plant less applied fertilizer compares, and cucumber quality and yield have carried
It is high.The result illustrates that a kind of preparation method for the microbial-bacterial fertilizer for promoting cucumber to take root of the present invention makes the well developed root system of cucumber, enters
And the nutrient absorption efficiency of cucumber is improved, the growth quality of cucumber is improved, there is important positive effect to agricultural production.
Claims (4)
- A kind of 1. microbial-bacterial fertilizer for promoting cucumber to take root, it is characterised in that：Described microbial-bacterial fertilizer is matched by following component Raw material be made：Agrobacterium rhizogenes bacterium solution 15mlOrganic matter composition 400gBiomass carbon 100gStrain contained by the agrobacterium rhizogenes liquid is conventional Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022, One or more in 15834, strain derive from Chinese microorganism strain collection；The organic matter composition is fermentation clover slag, fermentation mushroom residue, fermented maize stalk, one kind or several in animal wastes Kind；The biomass carbon refers to timber, grass, maize straw or other agricultural waste materials under low-oxygen environment by high anneal crack The material that solution carbonization is formed.
- A kind of 2. microbial-bacterial fertilizer for promoting cucumber to take root according to claim 1, it is characterised in that：Described root of hair agriculture Bacillus bacterium solution is the agrobacterium rhizogenes bacterium solution that OD values are 0.4-0.6.
- A kind of 3. preparation method of microbial-bacterial fertilizer for promoting cucumber to take root according to claim 1, it is characterised in that：Should Method comprises the following steps：1) preparation of agrobacterium rhizogenes bacterium solution, by one in Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 Kind or several activate to OD values are 0.4-0.6, obtain agrobacterium rhizogenes bacterium solution.2) organic matter composition and biomass carbon are crushed to 40-60 mesh respectively, by organic matter composition and biomass carbon 4:1 is mixed Close the mixture for uniformly, obtaining organic matter composition and biomass carbon.3) the root of hair agriculture that step 1) prepares is added in organic matter composition and the mixture of biomass carbon prepared by step 2) Bacillus bacterium solution uniform stirring, the mixture of every 500g organic matters composition and biomass carbon add bacterium solution 15ml, obtain microbial bacteria Fertilizer.
- A kind of 4. preparation method of microbial-bacterial fertilizer for promoting cucumber to take root according to claim 3, it is characterised in that institute Effective viable bacteria >=3 × 10 of microbial-bacterial fertilizer stated8cuf/g。
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711339088.XA CN107827613A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer for promoting cucumber to take root and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711339088.XA CN107827613A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer for promoting cucumber to take root and preparation method thereof |
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| Publication Number | Publication Date |
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| CN107827613A true CN107827613A (en) | 2018-03-23 |
Family
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| CN201711339088.XA Pending CN107827613A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial-bacterial fertilizer for promoting cucumber to take root and preparation method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106431569A (en) * | 2016-09-28 | 2017-02-22 | 合肥沁牧生态农业有限公司 | Preparation method of water-cultivated flower seedling culture solution |
| CN107011050A (en) * | 2017-04-20 | 2017-08-04 | 内蒙古民族大学 | A kind of charcoal base dissolving phosphor and dissolving potassium bacterial manure and preparation method thereof |
| CN107278590A (en) * | 2017-07-28 | 2017-10-24 | 周运德 | Improve ginsenoside Rb in cultivation field seven1The method of content |
-
2017
- 2017-12-14 CN CN201711339088.XA patent/CN107827613A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106431569A (en) * | 2016-09-28 | 2017-02-22 | 合肥沁牧生态农业有限公司 | Preparation method of water-cultivated flower seedling culture solution |
| CN107011050A (en) * | 2017-04-20 | 2017-08-04 | 内蒙古民族大学 | A kind of charcoal base dissolving phosphor and dissolving potassium bacterial manure and preparation method thereof |
| CN107278590A (en) * | 2017-07-28 | 2017-10-24 | 周运德 | Improve ginsenoside Rb in cultivation field seven1The method of content |
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Application publication date: 20180323 |