CN107823112A - A kind of anti-oxidant skin care item containing pot marigold stem cell extract - Google Patents
A kind of anti-oxidant skin care item containing pot marigold stem cell extract Download PDFInfo
- Publication number
- CN107823112A CN107823112A CN201711280731.6A CN201711280731A CN107823112A CN 107823112 A CN107823112 A CN 107823112A CN 201711280731 A CN201711280731 A CN 201711280731A CN 107823112 A CN107823112 A CN 107823112A
- Authority
- CN
- China
- Prior art keywords
- extract
- stem cell
- pot marigold
- parts
- skin care
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of antioxidant composition, the composition contains the composition of following parts by weight：0.8~8.7 part of 0.06~4.1 part of pot marigold stem cell extract, 0.7~6.4 part of Radix seu Caulis Parabarii extract, 1.1~8.8 parts of ginkgo biloba p.e, 0.9~9.3 part of Cedarwood extract and Chinese toon extract.Meanwhile the invention also discloses the skin care item containing above-mentioned composition.The skin care item of the present invention contain pot marigold stem cell extract, Radix seu Caulis Parabarii extract, ginkgo biloba p.e, Cedarwood extract and Chinese toon extract, have good oxidation resistant effect；In addition, the skin care item of the present invention are free of preservative, nontoxic to skin safe, fungistatic effect is good.
Description
Technical field
The present invention relates to technical field of skin care, shield is nourished in especially a kind of conditioning containing pot marigold stem cell extract
Skin product.
Background technology
Skin oxidative is due to that skin is chronically exposed to sunlight, foul atmosphere and the lower oxidation reaction occurred of computer radiation,
The skin that it can allow original year light-water tender becomes coarse and relaxation.Exposure to Sunlight ultraviolet is the number one killer for making skin oxidative, ultraviolet
Line UVA and UVB can produce a kind of hazardous compound after skin is infiltrated through --- free radical, destroy skin collagen and
Elastomer；After skin oxidative, its strong oxidizing property can damage the tissue and cell of body, cause collagen and elastomer pine
Relaxation and embrittlement, wrinkle is produced, makes cholesteroderma blackening.Nucleus is injured by free radical, just can not divide the thin of health
Born of the same parents.Infringement moment of the free radical to skin is occurring, and this is the root of our skin agings, and why skin wants antioxygen
The reason for change.
On October 25th, 2010, Nature Biotechnology have delivered the research institute of the Unhwa Corp companies of South Korea
It is successfully separated first and has cultivated the paper of plant stem cell, and certification has been obtained to the identity of plant stem cell, plant does
The research of cell enters the stage of high speed development.
In simple terms, there are two " group " cells for being located at plant tip in plant, they have powerful self-renewing
Ability, it can not only be divided into autogenous cell, moreover it is possible to other any plant cells are divided into, are qualified " general-purpose cells ",
Moreover, they, which also have, continues splitting ability so that many large-scale plant existences more than thousand.
2012, " plant stem cell " technology was studied in the effect of beauty and health care field and has been achieved for certain progress,
Metabolism of the plant stem cell to raising skin is proved, improves microcirculation, tightening up and recovering skin elasticity has major application
Value.
The content of the invention
Based on this, providing one kind it is an object of the invention to overcome in place of above-mentioned the deficiencies in the prior art can effective antioxygen
The skin care item of change, the skin care item are safe and non-toxic to skin, will not cause allergic.
To achieve the above object, this invention takes following technical scheme：
As the first aspect of the invention, the invention provides a kind of antioxidant composition, the composition contain with
The composition of lower parts by weight：0.06~4.1 part of pot marigold stem cell extract, 0.7~6.4 part of Radix seu Caulis Parabarii extract, ginkgo leaf carry
Take 0.8~8.7 part of 1.1~8.8 parts of thing, 0.9~9.3 part of Cedarwood extract and Chinese toon extract.
Wherein, pot marigold is rich in multivitamin, especially vitamin A and vitamin C, can prevent pigementation, promote
Skin gloss and elasticity, delaying senility, avoid the raw wrinkle of skin relaxation；It can also stimulate circulation；Also alcoholism can be relaxed, therefore
Also beneficial to tonifying liver the effect of；Pot marigold stem cell can play interference effect by epigenetic factor pair human skin cell,
The power of regeneration of skin progenitor cell can be effectively encouraged, hyperplasia fibrillar structure albumen, accelerates self more raw and reparation of skin；
Red bark of eucommia complete stool can hyoscine, Guangxi is among the people to be had for controlling the diseases such as polio, traumatic injury, and its emulsion can control wind
Wet pelvic bone pain；
Edible ginkgo nut can be with restraining and sterilizing bacteria, disease of dispelling cough-relieving, waterlogging-resistant suppression worm, check with turbidity and reduction serum cholesterol；In addition,
Ginkgo can reduce level of lipid, reduce freckle, skin of moistening, beautiful appearance.Glycosides displayed and flavones in ginkgo leaf
Alcohol is all the street cleaner of free radical, can protect corium confluent monolayer cells, improves blood circulation, prevents cell is oxidized from producing wrinkle；Silver
Apricot is one of herbal medicine with resistant activity gene ability, and ginkgo hinders in protection lipid (part of cell membrane) from free radical
Evil aspect is very effective；
Deodar sterilization, it is anti-grease leakage and gentle astriction is their ability to effective treatment of dandruff and scalp itch disease of releiving
The reason for shape；Convergent effect can help to treat acne and oiliness fash；Deodar also can effectively eliminating the phlegm and resolving sputum, in tradition
On be used to treat mucous membrane problem always, particularly bronchial infection and obstruction-it can clear up the excessive phlegm of respiratory system and
Mucus, it is also stated that highly effective；Deodar can be used for the symptoms such as arthritis and rheumatism, can nourish whole body；For psychology with
And the symptom such as stress, anxiety, obsession and fear also has the excellent effect of releiving；
Contain vitamin E and sex hormone material in Chinese toon, there is anti-aging and yang-tonifying nourishing yin effect, have one to infertility
Constant current modulation is imitated, therefore has the laudatory title of " gestogen ".Chinese toon is seasonal famous-object, can invigorating the spleen containing the volatile aromatic organic matter such as toonin
Appetizing, increase appetite；The Volatilized smell of Chinese toon can pass through the epidermis of roundworm, roundworm is discharged body from being attached in intestinal wall
Outside, can be with controlling roundworm disease；Chinese toon contains abundant vitamin C, carrotene etc., helps to strengthen body's immunity, and have profit
The effect of sliding skin, it is the good food of health and beauty.
Preferably, the mass ratio of the pot marigold stem cell extract, Radix seu Caulis Parabarii extract and ginkgo biloba p.e is
0.13~1.5：3.2：2.7~5.9.It is highly preferred that pot marigold stem cell extract, Radix seu Caulis Parabarii extract and the ginkgo leaf carry
The mass ratio for taking thing is 0.3：3.2：3.5.Present inventor has found through test of many times, when pot marigold stem cell in skin care item
The mass ratio of extract, Radix seu Caulis Parabarii extract and ginkgo biloba p.e is 0.13~1.5：3.2：When 2.7~5.9, skin care item
Oxidation resistance is preferable；When the quality of pot marigold stem cell extract, Radix seu Caulis Parabarii extract and ginkgo biloba p.e in skin care item
Than for 0.3：3.2：When 3.5, the oxidation resistance of skin care item is more preferably.
Preferably, the mass ratio of the pot marigold stem cell extract and Chinese toon extract is 0.11~2.1：4.2.It is more excellent
Selection of land, the mass ratio of the pot marigold stem cell extract and Chinese toon extract is 0.25：4.2.Present inventor's warp is more
Secondary experiment is found, when the mass ratio of pot marigold stem cell extract and Chinese toon extract is 0.11~2.1 in skin care item：When 4.2,
The fungistatic effect of skin care item is preferable；And work as the mass ratio of pot marigold stem cell extract and Chinese toon extract in skin care item and be
0.25：When 4.2, the fungistatic effect of skin care item is more preferably.
Preferably, the composition contains the composition of following parts by weight：0.3 part of pot marigold stem cell extract, red bark of eucommia
5.04 parts of 3.2 parts of extract, 3.5 parts of ginkgo biloba p.e, 5.2 parts of Cedarwood extract and Chinese toon extract.Present invention
People has found through test of many times, when containing 0.3 part of pot marigold stem cell extract, 3.2 parts of Radix seu Caulis Parabarii extract, ginkgo in skin care item
When 5.04 parts of 3.5 parts of leaf extract, 5.2 parts of Cedarwood extract and Chinese toon extract, the anti-oxidant and fungistatic effect of skin care item is all
It is good.
As the second aspect of the invention, the invention provides a kind of anti-oxidant skin care item, the skin care item include upper
The composition stated.
Preferably, pot marigold stem cell extract, Radix seu Caulis Parabarii extract, ginkgo biloba p.e, deodar in the skin care item
The quality percentage total amount of extract and Chinese toon extract is 8.6~10.9%.Present inventor has found through test of many times, when
Pot marigold stem cell extract in skin care item, Radix seu Caulis Parabarii extract, ginkgo biloba p.e, Cedarwood extract and Chinese toon extract
When quality percentage total amount is 8.6~10.9%, the oxidation resistance of skin care item is more preferably.
Preferably, the pot marigold stem cell extract is prepared by the following：
S1, take pot marigold new life root or branch to sterilize, after removal xylem and marrow, be inoculated in inducing culture, induce
Culture obtains cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained pot marigold stem cell obtain described pot marigold stem cell extract through the micro-nano method broken wall of low temperature.
Wherein, the micro-nano method broken wall of low temperature refers to make its freezing in a low temperature of pot marigold stem cell is placed on into -170 DEG C, with
Melt at normal temperatures afterwards, so repeatedly so that the cell membrane of pot marigold stem cell crushes；This method can live the life of stem cell
Property with power of regeneration assign skin care item in, absorbed via skin, can remarkably promote nutrition penetrate into and absorb, can effectively lift skin
Initiative, improve skin quality, the oxidation of delaying skin from cell aspect.
Preferably, the Radix seu Caulis Parabarii extract is prepared by the following：Red bark of eucommia leaf is adopted after high speed bead mill method broken wall
Radix seu Caulis Parabarii extract is obtained with water extraction；
The ginkgo biloba p.e is prepared by the following：Ginkgo leaf is taken to be obtained after ultrasonication using alcohol extracting method
Ginkgo biloba p.e；
The Cedarwood extract is prepared by the following：Cedar leaf is taken after the micro-nano method broken wall of low temperature, using alcohol extracting
Method obtains Cedarwood extract；
The Chinese toon extract is prepared by the following：Chinese Toon Leaves are taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains Chinese toon extract.Wherein, alcohol extracting method is preferably extracted using ethanol or methanol.
Relative to prior art, beneficial effects of the present invention are：
The skin care item of the present invention contain pot marigold stem cell extract, Radix seu Caulis Parabarii extract, ginkgo biloba p.e, deodar and carried
Thing and Chinese toon extract are taken, there is good oxidation resistant effect；In addition, the skin care item of the present invention are free of preservative, to skin
Safe and non-toxic, fungistatic effect is good.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of a kind of embodiment of antioxidant composition in the present invention, the composition contain following parts by weight into
Point：0.06 part of pot marigold stem cell extract, 6.4 parts of Radix seu Caulis Parabarii extract, 1.1 parts of ginkgo biloba p.e, Cedarwood extract 9.3
0.8 part of part and Chinese toon extract.
A kind of embodiment of anti-oxidant skin care item in the present invention, the skin care item include above-mentioned composition, 45 parts of saualane,
122 parts of 23 parts of butanediol, 55 parts of Arabic gum and water, wherein, pot marigold stem cell extract, red bark of eucommia in the skin care item
Extract, ginkgo biloba p.e, the quality percentage total amount of Cedarwood extract and Chinese toon extract are 7.9%.
The specific preparation method of the above-mentioned anti-oxidant skin care item of tune comprises the following steps：
(1) pot marigold stem cell extract is prepared by the following：
S1, after taking the sterilization of pot marigold new life branch, removal xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained pot marigold stem cell obtain described pot marigold stem cell extract through the micro-nano method broken wall of low temperature.
(2) Radix seu Caulis Parabarii extract is prepared by the following：Red bark of eucommia leaf is after high speed bead mill method broken wall, using water extraction
Obtain Radix seu Caulis Parabarii extract；
Ginkgo biloba p.e is prepared by the following：Ginkgo leaf is taken to be obtained after ultrasonication using methanol extraction
Ginkgo biloba p.e；
Cedarwood extract is prepared by the following：Cedar leaf is taken to be extracted after the micro-nano method broken wall of low temperature using ethanol
Method obtains Cedarwood extract；
Chinese toon extract is prepared by the following：Chinese Toon Leaves are taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To Chinese toon extract.
(3) by Radix seu Caulis Parabarii extract, ginkgo biloba p.e, Cedarwood extract and Chinese toon extract and 45 parts of saualane, fourth
122 parts of 23 parts of glycol, 55 parts of Arabic gum and water mixing, after 36 degrees Celsius stirs, are filtered to remove excessive moisture, obtain
To lotion, then addition pot marigold stem cell extract, which is uniformly dispersed, produces a kind of anti-oxidant face cream.
Embodiment 2
A kind of a kind of embodiment of antioxidant composition in the present invention, the composition contain following parts by weight into
Point：0.06 part of pot marigold stem cell extract, 6.4 parts of Radix seu Caulis Parabarii extract, 1.1 parts of ginkgo biloba p.e, Cedarwood extract 9.3
0.8 part of part and Chinese toon extract.
A kind of embodiment of anti-oxidant skin care item in the present invention, the skin care item include above-mentioned composition, 48 parts of saualane,
101 parts of 27 parts of butanediol, 51 parts of Arabic gum and water, wherein, pot marigold stem cell extract, red bark of eucommia in the skin care item
Extract, ginkgo biloba p.e, the quality percentage total amount of Cedarwood extract and Chinese toon extract are 8.6%.
The specific preparation method of above-mentioned anti-oxidant skin care item comprises the following steps：
(1) pot marigold stem cell extract is prepared by the following：
S1, after taking the sterilization of pot marigold new life root, removal xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained pot marigold stem cell obtain described pot marigold stem cell extract through the micro-nano method broken wall of low temperature.
(2) Radix seu Caulis Parabarii extract is prepared by the following：Red bark of eucommia leaf is after high speed bead mill method broken wall, using water extraction
Obtain Radix seu Caulis Parabarii extract；
Ginkgo biloba p.e is prepared by the following：Ginkgo leaf is taken to be obtained after ultrasonication using ethanol extraction method
Ginkgo biloba p.e；
Cedarwood extract is prepared by the following：Cedar leaf is taken to be extracted after the micro-nano method broken wall of low temperature using methanol
Method obtains Cedarwood extract；
Chinese toon extract is prepared by the following：Chinese Toon Leaves are taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To Chinese toon extract.
(3) by Radix seu Caulis Parabarii extract, ginkgo biloba p.e, Cedarwood extract and Chinese toon extract and 48 parts of saualane, fourth
101 parts of 27 parts of glycol, 51 parts of Arabic gum and water mixing, after 36.5 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition pot marigold stem cell extract, which is uniformly dispersed, produces a kind of anti-oxidant face cream.
Embodiment 3
A kind of a kind of embodiment of antioxidant composition in the present invention, the composition contain following parts by weight into
Point：0.06 part of pot marigold stem cell extract, 6.4 parts of Radix seu Caulis Parabarii extract, 1.1 parts of ginkgo biloba p.e, Cedarwood extract 9.3
0.8 part of part and Chinese toon extract.
A kind of embodiment of anti-oxidant skin care item in the present invention, the skin care item include above-mentioned composition, 36 parts of saualane,
88 parts of 18 parts of butanediol, 42 parts of Arabic gum and water, wherein, pot marigold stem cell extract, red bark of eucommia in the skin care item
Extract, ginkgo biloba p.e, the quality percentage total amount of Cedarwood extract and Chinese toon extract are 9.4%.
The specific preparation method of above-mentioned anti-oxidant skin care item comprises the following steps：
(1) pot marigold stem cell extract is prepared by the following：
S1, after taking the sterilization of pot marigold new life branch, removal xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained pot marigold stem cell obtain described pot marigold stem cell extract through the micro-nano method broken wall of low temperature.
(2) Radix seu Caulis Parabarii extract is prepared by the following：Red bark of eucommia leaf is after high speed bead mill method broken wall, using water extraction
Obtain Radix seu Caulis Parabarii extract；
Ginkgo biloba p.e is prepared by the following：Ginkgo leaf is taken to be obtained after ultrasonication using ethanol extraction method
Ginkgo biloba p.e；
Cedarwood extract is prepared by the following：Cedar leaf is taken to be extracted after the micro-nano method broken wall of low temperature using ethanol
Method obtains Cedarwood extract；
Chinese toon extract is prepared by the following：Chinese Toon Leaves are taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To Chinese toon extract.
(3) by Radix seu Caulis Parabarii extract, ginkgo biloba p.e, Cedarwood extract and Chinese toon extract and 36 parts of saualane, fourth
88 parts of 18 parts of glycol, 42 parts of Arabic gum and water mixing, after 36.8 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition pot marigold stem cell extract, which is uniformly dispersed, produces a kind of anti-oxidant face cream.
Embodiment 4
A kind of a kind of embodiment of antioxidant composition in the present invention, the composition contain following parts by weight into
Point：0.06 part of pot marigold stem cell extract, 6.4 parts of Radix seu Caulis Parabarii extract, 1.1 parts of ginkgo biloba p.e, Cedarwood extract 9.3
0.8 part of part and Chinese toon extract.
A kind of embodiment of anti-oxidant skin care item in the present invention, the skin care item include above-mentioned composition, 52 parts of saualane,
111 parts of 31 parts of butanediol, 54 parts of Arabic gum and water, wherein, pot marigold stem cell extract, red bark of eucommia in the skin care item
Extract, ginkgo biloba p.e, the quality percentage total amount of Cedarwood extract and Chinese toon extract are 10.3%.
The specific preparation method of above-mentioned anti-oxidant skin care item comprises the following steps：
(1) pot marigold stem cell extract is prepared by the following：
S1, after taking the sterilization of pot marigold new life root, removal xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained pot marigold stem cell obtain described pot marigold stem cell extract through the micro-nano method broken wall of low temperature.
(2) Radix seu Caulis Parabarii extract is prepared by the following：Red bark of eucommia leaf is after high speed bead mill method broken wall, using water extraction
Obtain Radix seu Caulis Parabarii extract；
Ginkgo biloba p.e is prepared by the following：Ginkgo leaf is taken to be obtained after ultrasonication using methanol extraction
Ginkgo biloba p.e；
Cedarwood extract is prepared by the following：Cedar leaf is taken to be extracted after the micro-nano method broken wall of low temperature using methanol
Method obtains Cedarwood extract；
Chinese toon extract is prepared by the following：Chinese Toon Leaves are taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To Chinese toon extract.
(3) by Radix seu Caulis Parabarii extract, ginkgo biloba p.e, Cedarwood extract and Chinese toon extract and 52 parts of saualane, fourth
111 parts of 31 parts of glycol, 54 parts of Arabic gum and water mixing, after 37.1 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition pot marigold stem cell extract, which is uniformly dispersed, produces a kind of anti-oxidant face cream.
Embodiment 5
A kind of a kind of embodiment of antioxidant composition in the present invention, the composition contain following parts by weight into
Point：0.06 part of pot marigold stem cell extract, 6.4 parts of Radix seu Caulis Parabarii extract, 1.1 parts of ginkgo biloba p.e, Cedarwood extract 9.3
0.8 part of part and Chinese toon extract.
A kind of embodiment of anti-oxidant skin care item in the present invention, the skin care item include above-mentioned composition, 46 parts of saualane,
107 parts of 25 parts of butanediol, 55 parts of Arabic gum and water, wherein, pot marigold stem cell extract, red bark of eucommia in the skin care item
Extract, ginkgo biloba p.e, the quality percentage total amount of Cedarwood extract and Chinese toon extract are 10.9%.
The specific preparation method of above-mentioned anti-oxidant skin care item comprises the following steps：
(1) pot marigold stem cell extract is prepared by the following：
S1, after taking the sterilization of pot marigold new life branch, removal xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained pot marigold stem cell obtain described pot marigold stem cell extract through the micro-nano method broken wall of low temperature.
(2) Radix seu Caulis Parabarii extract is prepared by the following：Red bark of eucommia leaf is after high speed bead mill method broken wall, using water extraction
Obtain Radix seu Caulis Parabarii extract；
Ginkgo biloba p.e is prepared by the following：Ginkgo leaf is taken to be obtained after ultrasonication using ethanol extraction method
Ginkgo biloba p.e；
Cedarwood extract is prepared by the following：Cedar leaf is taken to be extracted after the micro-nano method broken wall of low temperature using ethanol
Method obtains Cedarwood extract；
Chinese toon extract is prepared by the following：Chinese Toon Leaves are taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To Chinese toon extract.
(3) by Radix seu Caulis Parabarii extract, ginkgo biloba p.e, Cedarwood extract and Chinese toon extract and 46 parts of saualane, fourth
107 parts of 25 parts of glycol, 55 parts of Arabic gum and water mixing, after 36.4 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition pot marigold stem cell extract, which is uniformly dispersed, produces a kind of anti-oxidant face cream.
The antioxidant effect test of the skin care item of the present invention of embodiment 6
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, the shield of control group 1~4
Differ only in pot marigold stem cell extract, Radix seu Caulis Parabarii extract and the ginkgo leaf of skin frost and the face cream of embodiment 3 extract
The mass ratio of thing is different, and its preparation method is same as Example 3；The pot marigold stem cell extraction of the face cream of control group 1~4
The mass ratio of thing, Radix seu Caulis Parabarii extract and ginkgo biloba p.e is followed successively by：0.13：3.2：2.7；0.7：3.2：4.2；1.1：3.2：
5.1；1.5：3.2：5.9；
Method of testing：ORAC methods；Specifically, the face cream of Example 1~5 and 1~4 skin care as a control group respectively
Frost, it is configured to the aqueous solution, it is desirable to which extract total concentration is identical in the face cream aqueous solution, such as 1.4g/L, is used respectively using preceding
83mmol/L phosphate buffers (pH=7.2) dilute 1000 times and are used as test sample solution.
Test result：It is as shown in table 1 below；
The oxidation resistance test result of the face cream of the present invention of table 1
| Face cream | ORAC values | Face cream | ORAC values |
| Embodiment 1 | 668 | Control group 1 | 945 |
| Embodiment 2 | 973 | Control group 2 | 1162 |
| Embodiment 3 | 1443 | Control group 3 | 1093 |
| Embodiment 4 | 870 | Control group 4 | 1028 |
| Embodiment 5 | 834 |
Interpretation of result：It was found from upper table 1, the ORAC values of the face cream of embodiment 2,3 and control group 1~4 are higher, represent
The oxidation resistance of the face cream of embodiment 2 and 3 and control group 1~4 is preferable；Wherein, with the antioxygen of the face cream of embodiment 3
Change ability is optimal.
The fungistatic effect test of the skin care item of the present invention of embodiment 7
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, the shield of control group 1~4
Skin frost is different from the mass ratio for differing only in pot marigold stem cell extract and Chinese toon extract of the face cream of embodiment 3,
Its preparation method is same as Example 3；Pot marigold stem cell extract and Chinese toon extract in the face cream of control group 1~4
Mass ratio is 0.11 successively：4.2；0.83：4.2；1.45：4.2；2.1：4.2；
Method of testing：Choose staphylococcus, streptococcus, bacterium acidi propionici and corynebacteria, and LB solid mediums (i.e. LB
Flat board), face cream is dissolved into the aqueous solution that mass percent is 3.1% with water, by staphylococcus, streptococcus, bacterium acidi propionici
With corynebacteria according to quantity 1：1：1：Added after 1 mixing in the aqueous solution of face cream and form mixed solution, bacterium in mixed solution
Concentration be 1.1 × 106It is individual, drip 7 drop mixed solutions on LB flat boards with dropper respectively, the straight of bacterial plaque is measured after 52 hours
Footpath, and calculate bacterial plaque average diameter.
Test result：It is as shown in table 2 below；
The fungistatic effect of the face cream of the present invention of table 2
| Face cream | Bacterial plaque diameter | Face cream | Bacterial plaque diameter |
| Embodiment 1 | 0.22cm | Control group 1 | 0.19cm |
| Embodiment 2 | 0.52cm | Control group 2 | 0.15cm |
| Embodiment 3 | 0cm | Control group 3 | 0.26cm |
| Embodiment 4 | 0.33cm | Control group 4 | 0.45cm |
| Embodiment 5 | 0.41cm |
Interpretation of result：As shown in upper table 2, it is known that the face cream of embodiment 1~5 and control group 1~4 all has one
Fixed fungistatic effect, wherein, using the antibacterial ability of the face cream of embodiment 3 to be best.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of antioxidant composition, it is characterised in that the composition contains the composition of following parts by weight：Pot marigold stem cell
0.06~4.1 part of extract, 0.7~6.4 part of Radix seu Caulis Parabarii extract, 1.1~8.8 parts of ginkgo biloba p.e, Cedarwood extract 0.9
~9.3 parts and 0.8~8.7 part of Chinese toon extract.
2. composition according to claim 1, it is characterised in that the pot marigold stem cell extract, red bark of eucommia extraction
The mass ratio of thing and ginkgo biloba p.e is 0.13~1.5：3.2：2.7~5.9.
3. composition according to claim 2, it is characterised in that the pot marigold stem cell extract, red bark of eucommia extraction
The mass ratio of thing and ginkgo biloba p.e is 0.3：3.2：3.5.
4. composition according to claim 1, it is characterised in that the pot marigold stem cell extract and Chinese toon extract
Mass ratio be 0.11~2.1：4.2.
5. composition according to claim 4, it is characterised in that the pot marigold stem cell extract and Chinese toon extract
Mass ratio be 0.25：4.2.
6. composition according to claim 1, it is characterised in that the composition contains the composition of following parts by weight：Gold
0.3 part of small cup flower stem cell extract, 3.2 parts of Radix seu Caulis Parabarii extract, 3.5 parts of ginkgo biloba p.e, 5.2 parts of Cedarwood extract and perfume
5.04 parts of Chinese toon extract.
7. a kind of anti-oxidant skin care item, it is characterised in that the skin care item include the group as described in any one of claim 1~6
Compound.
8. skin care item according to claim 7, it is characterised in that pot marigold stem cell extract in the skin care item, red
Eucommia ulmoides extracts, ginkgo biloba p.e, the quality percentage total amount of Cedarwood extract and Chinese toon extract are 8.6~10.9%.
9. skin care item according to claim 7, it is characterised in that the pot marigold stem cell extract is by the following method
Obtain：
S1, take pot marigold new life root or branch to sterilize, after removal xylem and marrow, be inoculated in inducing culture, Fiber differentiation
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained pot marigold stem cell obtain described pot marigold stem cell extract through the micro-nano method broken wall of low temperature.
10. skin care item according to claim 7, it is characterised in that the Radix seu Caulis Parabarii extract is prepared by the following：
Red bark of eucommia leaf obtains Radix seu Caulis Parabarii extract after high speed bead mill method broken wall using water extraction；
The ginkgo biloba p.e is prepared by the following：Ginkgo leaf is taken after ultrasonication, ginkgo is obtained using alcohol extracting method
Leaf extract；
The Cedarwood extract is prepared by the following：Cedar leaf is taken to be obtained after the micro-nano method broken wall of low temperature using alcohol extracting method
To Cedarwood extract；
The Chinese toon extract is prepared by the following：Chinese Toon Leaves are taken to be obtained after the micro-nano method broken wall of low temperature using water extraction
To Chinese toon extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711280731.6A CN107823112A (en) | 2017-12-06 | 2017-12-06 | A kind of anti-oxidant skin care item containing pot marigold stem cell extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711280731.6A CN107823112A (en) | 2017-12-06 | 2017-12-06 | A kind of anti-oxidant skin care item containing pot marigold stem cell extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107823112A true CN107823112A (en) | 2018-03-23 |
Family
ID=61641711
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711280731.6A Withdrawn CN107823112A (en) | 2017-12-06 | 2017-12-06 | A kind of anti-oxidant skin care item containing pot marigold stem cell extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107823112A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110652473A (en) * | 2018-06-29 | 2020-01-07 | 伽蓝（集团）股份有限公司 | Application of cedar extract |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099768A (en) * | 2007-07-25 | 2008-01-09 | 天津科技大学 | Method for extracting anti-oxidation active substance from overgrown leaves of Chinese toon |
| CN104711215A (en) * | 2015-04-09 | 2015-06-17 | 广州赛莱拉干细胞科技股份有限公司 | Apple stem cell culture method and apple stem cells cultured by method |
| CN104738635A (en) * | 2015-04-13 | 2015-07-01 | 广州赛莱拉干细胞科技股份有限公司 | Antioxidative composition and preparation method thereof |
| CN105616273A (en) * | 2015-12-23 | 2016-06-01 | 庄信修 | Application of cortex parabarii and cortex parabarii extract to preparation of anti-aging skin care products |
| CN105616467A (en) * | 2015-12-23 | 2016-06-01 | 庄信修 | Application of cortex parabarii and cortex parabarii extract both serving as whitening effective ingredients |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
| CN105878118A (en) * | 2016-05-21 | 2016-08-24 | 广州丹奇日用化工厂有限公司 | Method for extracting cedrela sinensis extract from cedrela sinensis leaves and application of method |
-
2017
- 2017-12-06 CN CN201711280731.6A patent/CN107823112A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099768A (en) * | 2007-07-25 | 2008-01-09 | 天津科技大学 | Method for extracting anti-oxidation active substance from overgrown leaves of Chinese toon |
| CN104711215A (en) * | 2015-04-09 | 2015-06-17 | 广州赛莱拉干细胞科技股份有限公司 | Apple stem cell culture method and apple stem cells cultured by method |
| CN104738635A (en) * | 2015-04-13 | 2015-07-01 | 广州赛莱拉干细胞科技股份有限公司 | Antioxidative composition and preparation method thereof |
| CN105616273A (en) * | 2015-12-23 | 2016-06-01 | 庄信修 | Application of cortex parabarii and cortex parabarii extract to preparation of anti-aging skin care products |
| CN105616467A (en) * | 2015-12-23 | 2016-06-01 | 庄信修 | Application of cortex parabarii and cortex parabarii extract both serving as whitening effective ingredients |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
| CN105878118A (en) * | 2016-05-21 | 2016-08-24 | 广州丹奇日用化工厂有限公司 | Method for extracting cedrela sinensis extract from cedrela sinensis leaves and application of method |
Non-Patent Citations (1)
| Title |
|---|
| 王建新 等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110652473A (en) * | 2018-06-29 | 2020-01-07 | 伽蓝（集团）股份有限公司 | Application of cedar extract |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107737041A (en) | A kind of novel skin antiallergic maintenance composition, skin repair frost and preparation method thereof | |
| CN108670943A (en) | A kind of moisturizing, whitening, light spot composition of plant extracts | |
| KR101926263B1 (en) | Cosmetic compositions containing fermented extract of Origanum majorana, Corchorus olitorius and Melissa officianalis | |
| CN108309880A (en) | A kind of microspecies rose essence liquid and preparation method thereof | |
| CN108815072A (en) | A kind of plant composition fermentation magma and its preparation method and application | |
| CN111568780A (en) | Herbal gel with myotonin and preparation method thereof | |
| CN104622756A (en) | Extraction method of grape flower cell extract and application of extraction method | |
| CN109303724A (en) | A kind of novel maintenance stoste composition of releiving containing folium artemisiae argyi | |
| CN109381390A (en) | A kind of sodium hyaluronate skin lightening Face-protecting mask and preparation method thereof | |
| CN108186542A (en) | Lip care lipstick with antiallergic effect of releiving | |
| KR20160050527A (en) | Composition of cosmetics for anti-wrinkle containing Ulva pertusa Kjellman extracts | |
| CN107595688A (en) | A kind of sun-screening agent and its application | |
| CN107823112A (en) | A kind of anti-oxidant skin care item containing pot marigold stem cell extract | |
| KR101746277B1 (en) | Manufacturing method for hair cosmetic | |
| CN110664700B (en) | Composition and application thereof in preparation of product for preventing and treating acne | |
| KR102054100B1 (en) | Cosmetic composition containing fermented extract of roots | |
| KR101116447B1 (en) | Cosmetic composition for activation of skin cell dynamics | |
| CN110840799A (en) | Whitening, moisturizing, antibacterial and mosquito-repellent body lotion and preparation method thereof | |
| KR20110116389A (en) | A skin-care agent containing fermented aloe and gastrodia elata | |
| CN107898751A (en) | Skin care item are nourished in a kind of conditioning containing Japanese Stephania Root stem cell extract | |
| CN107303255B (en) | Sorghum fermentation product having anti-inflammatory, anti-allergic and atopic skin improving effects, its preparation method and cosmetic composition | |
| CN116942569B (en) | Essential oil composition containing kuquai oil and application thereof | |
| CN104367539A (en) | Conditioning lotion containing cordyceps militaris ingredients and preparation method thereof | |
| CN107789310A (en) | Skin care item are nourished in a kind of conditioning containing Japanese Stephania Root stem cell extract | |
| KR102137952B1 (en) | Cosmetic composition with skin soothing effect |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180323 |
|
| WW01 | Invention patent application withdrawn after publication |