CN107789310A - Skin care item are nourished in a kind of conditioning containing Japanese Stephania Root stem cell extract - Google Patents
Skin care item are nourished in a kind of conditioning containing Japanese Stephania Root stem cell extract Download PDFInfo
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A61Q17/02—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings containing insect repellants
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The invention discloses one kind to nurse one's health tonic composition, and the composition contains the composition of following parts by weight：1.3~9.5 parts of 0.05~3.1 part of Japanese Stephania Root stem cell extract, 0.6~7.3 part of pot marigold extract, 0.9~7.8 part of oriental cherry extract, 1.1~8.4 parts of Western peony extract and dandelion extract.Meanwhile the invention also discloses the skin care item containing above-mentioned composition.The skin care item of the present invention contain Japanese Stephania Root stem cell extract, pot marigold extract, oriental cherry extract, Western peony extract and dandelion extract, have good oxidation resistant effect；In addition, the skin care item of the present invention are free of preservative, nontoxic to skin safe, fungistatic effect is good.
Description
Technical field
The present invention relates to technical field of skin care, shield is nourished in especially a kind of conditioning containing Japanese Stephania Root stem cell extract
Skin product.
Background technology
Skin oxidative is due to that skin is chronically exposed to sunlight, foul atmosphere and the lower oxidation reaction occurred of computer radiation,
The skin that it can allow original year light-water tender becomes coarse and relaxation.Exposure to Sunlight ultraviolet is the number one killer for making skin oxidative, ultraviolet
Line UVA and UVB can produce a kind of hazardous compound after skin is infiltrated through --- free radical, destroy skin collagen and
Elastomer；After skin oxidative, its strong oxidizing property can damage the tissue and cell of body, cause collagen and elastomer pine
Relaxation and embrittlement, wrinkle is produced, makes cholesteroderma blackening.Nucleus is injured by free radical, just can not divide the thin of health
Born of the same parents.Infringement moment of the free radical to skin is occurring, and this is the root of our skin agings, and why skin wants antioxygen
The reason for change.
Japanese Stephania Root, alias Radix Stephaniae Cepharanthae, public mouse rattan, wild peach grass, firecracker disappear, towards day ointment, close cymbal grass, spun gold lotus
Leaf, day plaster, it is the root or cauline leaf of Menispermaceae stephania plant Japanese Stephania Root；With clearing heat and detoxicating, wind-expelling pain-stopping, inducing diuresis for removing edema
The effect of；It is usually used in abscess of throat, carbuncle swells boil, venomous snake bite, arthralgia pain due to rheumatism, stomachache, tinea pedis oedema；Its pharmacological action bag
Include：1) Muscle relaxation；2) ganglionic blocking action；3) foreign study proves that Japanese Stephania Root plays the role of to prevent and treat leukopenia.
On October 25th, 2010, Nature Biotechnology have delivered the research institute of the Unhwa Corp companies of South Korea
It is successfully separated first and has cultivated the paper of plant stem cell, and certification has been obtained to the identity of plant stem cell, plant does
The research of cell enters the stage of high speed development.
In simple terms, there are two " group " cells for being located at plant tip in plant, they have powerful self-renewing
Ability, it can not only be divided into autogenous cell, moreover it is possible to other any plant cells are divided into, are qualified " general-purpose cells ",
Moreover, they, which also have, continues splitting ability so that many large-scale plant existences more than thousand.
2012, " plant stem cell " technology was studied in the effect of beauty and health care field and has been achieved for certain progress,
Metabolism of the plant stem cell to raising skin is proved, improves microcirculation, tightening up and recovering skin elasticity has major application
Value.
The content of the invention
Based on this, providing one kind it is an object of the invention to overcome in place of above-mentioned the deficiencies in the prior art can effective expelling parasite
Skin care item, the skin care item are safe and non-toxic to skin, will not cause allergic.
To achieve the above object, this invention takes following technical scheme：
As the first aspect of the invention, the invention provides one kind to nurse one's health tonic composition, and the composition contains
The composition of following parts by weight：0.05~3.1 part of Japanese Stephania Root stem cell extract, 0.6~7.3 part of pot marigold extract, oriental cherry carry
Take 1.3~9.5 parts of 0.9~7.8 part of thing, 1.1~8.4 parts of Western peony extract and dandelion extract.
Wherein, Japanese Stephania Root stem cell can play interference effect by epigenetic factor pair human skin cell, can have
The power of regeneration of effect excitation skin progenitor cell, hyperplasia fibrillar structure albumen, accelerate self more raw and reparation of skin；Pot marigold has anti-
Bacterium, antiinflammation, it is particularly preferable to staphylococcus, streptococcus effect；Oriental cherry has good pore refining and Sage Extract
The effect of, there is tender skin, lightening skin color, containing abundant natural vitamin A, B, E, cherry leaf flavones is also supported with beauty
Face, strengthen mucous membrane, promote the drug effect of sugar metabolism；Drink peony congee can with nourishing blood for regulating menstruation, control irritability is uncomfortable, weak constitution, debility and
See the diseases such as hypochondriac pain agitation, bellyache in menstrual period；Contain taraxol, taraxacin, choline, organic acid, synanthrin in dandelion plant
It is sweet etc. a variety of health-nutrition compositions, its nature and flavor, slight bitter, tremble with fear；There are diuresis, laxative, removing jaundice subcutaneous ulcer, cholagogic and other effects.
Preferably, the mass ratio of the Japanese Stephania Root stem cell extract, oriental cherry extract and Western peony extract is
0.08~1.4：2.3：3.6~5.9.It is highly preferred that Japanese Stephania Root stem cell extract, oriental cherry extract and the Western Chinese herbaceous peony carry
The mass ratio for taking thing is 0.4：2.3：4.5.Present inventor has found through test of many times, when Japanese Stephania Root stem cell in skin care item
The mass ratio of extract, oriental cherry extract and Western peony extract is 0.08~1.4：2.3：When 3.6~5.9, skin care item
Antioxidant effect is preferable；And when the matter of Japanese Stephania Root stem cell extract, oriental cherry extract and Western peony extract in skin care item
Amount is than being 0.4：2.3：When 4.5, the antioxidant effect of skin care item is more preferably.
Preferably, the mass ratio of the Japanese Stephania Root stem cell extract and pot marigold extract is 0.12~1.1：3.7.More
Preferably, the mass ratio of the Japanese Stephania Root stem cell extract and pot marigold extract is 0.33：3.7.Present inventor
Found through test of many times, when the mass ratio of Japanese Stephania Root stem cell extract and pot marigold extract is 0.12~1.1 in skin care item：
When 3.7, the fungistatic effect of skin care item is preferable；And when the quality of Japanese Stephania Root stem cell extract and pot marigold extract in skin care item
Than for 0.33：When 3.7, the fungistatic effect of skin care item is more preferably.
Preferably, the composition contains the composition of following parts by weight：0.4 part of Japanese Stephania Root stem cell extract, pot marigold
7.2 parts of 4.5 parts of extract, 2.3 parts of oriental cherry extract, 4.5 parts of Western peony extract and dandelion extract.The hair of the application
A person of good sense has found through test of many times, when containing 0.4 part of Japanese Stephania Root stem cell extract, 4.5 parts of pot marigold extract, cherry in skin care item
When 7.2 parts of 2.3 parts of flower extract, 4.5 parts of Western peony extract and dandelion extract, the anti-oxidant and antibacterial effect of skin care item
Fruit is all good.
As the second aspect of the invention, nursed one's health the invention provides one kind and nourish skin care item, the skin care item include
Combinations of the above thing.
Preferably, Japanese Stephania Root stem cell extract, pot marigold extract, oriental cherry extract, Western Chinese herbaceous peony in the skin care item
The quality percentage total amount of medicament extract and dandelion extract is 8.55~11.2%.Present inventor sends out through test of many times
It is existing, when Japanese Stephania Root stem cell extract, pot marigold extract, oriental cherry extract, Western peony extract and Pu are public in skin care item
When the quality percentage total amount of English extract is 8.55~11.2%, the oxidation resistance of skin care item is more preferably.
Preferably, the Japanese Stephania Root stem cell extract is prepared by the following：
S1, Japanese Stephania Root new life root or branch sterilization, after removing xylem and marrow, it is inoculated in inducing culture, induction training
Support and obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.Its
In, the micro-nano method broken wall of low temperature refers to make its freezing in a low temperature of Japanese Stephania Root stem cell is placed on into -170 DEG C, then at normal temperatures
Melt, so repeatedly so that the cell membrane of Japanese Stephania Root stem cell crushes；This method can be by the vital activity and Regenerated energy of stem cell
Power is assigned in skin care item, is absorbed via skin, can be remarkably promoted nutrition and be penetrated into and absorb, can effectively lift skin initiative, from
Cell aspect improves skin quality, the aging of delaying skin.
Preferably, the pot marigold extract is prepared by the following：Pot marigold uses after high speed bead mill method broken wall
Water extraction obtains pot marigold extract；
The oriental cherry extract is prepared by the following：Oriental cherry is taken after ultrasonication, oriental cherry is obtained using alcohol extracting method
Extract；
The Western peony extract is prepared by the following：Western peony is taken after the micro-nano method broken wall of low temperature,
Western peony extract is obtained using alcohol extracting method；
The dandelion extract is prepared by the following：Dandelion stem is taken after the micro-nano method broken wall of low temperature, is used
Water extraction obtains dandelion extract.Wherein, alcohol extracting method is preferably extracted using ethanol or methanol.
Relative to prior art, beneficial effects of the present invention are：
The skin care item of the present invention contain Japanese Stephania Root stem cell extract, pot marigold extract, oriental cherry extract, Western Chinese herbaceous peony
Extract and dandelion extract, there is good oxidation resistant effect；In addition, the skin care item of the present invention are free of preservative, it is right
Skin safe is nontoxic, and fungistatic effect is good.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of a kind of embodiment of conditioning tonic composition in the present invention, the composition contain following parts by weight into
Point：0.05 part of Japanese Stephania Root stem cell extract, 7.3 parts of pot marigold extract, 0.9 part of oriental cherry extract, Western peony extract
8.4 parts and 1.3 parts of dandelion extract.
A kind of embodiment of skin care item is nourished in conditioning in the present invention, and the skin care item include above-mentioned composition, saualane 35
Part, 21 parts of glycerine, 63 parts of Arabic gum and 101 parts of mixing of water, wherein, Japanese Stephania Root stem cell extract, pot marigold extraction
The percentage that thing, oriental cherry extract, the gross mass of Western peony extract and dandelion extract account for skin care quality is
8.4%.
The specific preparation method that skin care item are nourished in above-mentioned conditioning comprises the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
(2) pot marigold extract is prepared by the following：Pot marigold is obtained after high speed bead mill method broken wall using water extraction
To pot marigold extract；
Oriental cherry extract is prepared by the following：Oriental cherry is taken after ultrasonication, oriental cherry is obtained using methanol extraction
Extract；
Western peony extract is prepared by the following：Western peony is taken after the micro-nano method broken wall of low temperature, is used
Ethanol extraction method obtains Western peony extract；
Dandelion extract is prepared by the following：Dandelion stem is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains dandelion extract.
(3) by pot marigold extract, oriental cherry extract, Western peony extract and dandelion extract and saualane 35
Part, 21 parts of glycerine, 63 parts of Arabic gum and 101 parts of mixing of water, after 36 degrees Celsius stirs, are filtered to remove superfluous water
Point, lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of conditioning nourishing face cream.
Embodiment 2
A kind of a kind of embodiment of conditioning tonic composition in the present invention, the composition contain following parts by weight into
Point：1.1 parts of Japanese Stephania Root stem cell extract, 6.1 parts of pot marigold extract, 4.1 parts of oriental cherry extract, Western peony extract
6.6 parts and 3.5 parts of dandelion extract.
A kind of embodiment of skin care item is nourished in conditioning in the present invention, and the skin care item include above-mentioned composition, saualane 43
Part, 25 parts of glycerine, 55 parts of Arabic gum and 96 parts of mixing of water, wherein, Japanese Stephania Root stem cell extract, pot marigold extraction
The percentage that thing, oriental cherry extract, the gross mass of Western peony extract and dandelion extract account for skin care quality is
9.98%.
The specific preparation method that skin care item are nourished in above-mentioned conditioning comprises the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
(2) pot marigold extract is prepared by the following：Pot marigold is obtained after high speed bead mill method broken wall using water extraction
To pot marigold extract；
Oriental cherry extract is prepared by the following：Oriental cherry is taken after ultrasonication, oriental cherry is obtained using ethanol extraction method
Extract；
Western peony extract is prepared by the following：Western peony is taken after the micro-nano method broken wall of low temperature, is used
Methanol extraction obtains Western peony extract；
Dandelion extract is prepared by the following：Dandelion stem is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains dandelion extract.
(3) by pot marigold extract, oriental cherry extract, Western peony extract and dandelion extract and saualane 43
Part, 25 parts of glycerine, 55 parts of Arabic gum and 96 parts of mixing of water, after 38 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of conditioning nourishing face cream.
Embodiment 3
A kind of a kind of embodiment of conditioning tonic composition in the present invention, the composition contain following parts by weight into
Point：0.4 part of Japanese Stephania Root stem cell extract, 4.5 parts of pot marigold extract, 2.3 parts of oriental cherry extract, Western peony extract
4.5 parts and 7.2 parts of dandelion extract.
A kind of embodiment of skin care item is nourished in conditioning in the present invention, and the skin care item include above-mentioned composition, saualane 35
Part, 20 parts of glycerine, 47 parts of Arabic gum and 88 parts of mixing of water, wherein, Japanese Stephania Root stem cell extract, pot marigold extraction
The percentage that thing, oriental cherry extract, the gross mass of Western peony extract and dandelion extract account for skin care quality is 10%.
The specific preparation method that skin care item are nourished in above-mentioned conditioning comprises the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
(2) pot marigold extract is prepared by the following：Pot marigold is obtained after high speed bead mill method broken wall using water extraction
To pot marigold extract；
Oriental cherry extract is prepared by the following：Oriental cherry is taken after ultrasonication, oriental cherry is obtained using ethanol extraction method
Extract；
Western peony extract is prepared by the following：Western peony is taken after the micro-nano method broken wall of low temperature, is used
Ethanol extraction method obtains Western peony extract；
Dandelion extract is prepared by the following：Dandelion stem is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains dandelion extract.
(3) by pot marigold extract, oriental cherry extract, Western peony extract and dandelion extract and saualane 35
Part, 20 parts of glycerine, 47 parts of Arabic gum and 88 parts of mixing of water, after 36.5 degrees Celsius stir, it is unnecessary to be filtered to remove
Moisture, lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of conditioning nourishing face cream.
Embodiment 4
A kind of a kind of embodiment of conditioning tonic composition in the present invention, the composition contain following parts by weight into
Point：2.5 parts of Japanese Stephania Root stem cell extract, 2.2 parts of pot marigold extract, 5.9 parts of oriental cherry extract, Western peony extract
2.3 parts and 5.8 parts of dandelion extract.
A kind of embodiment of skin care item is nourished in conditioning in the present invention, and the skin care item include above-mentioned composition, saualane 40
Part, 25 parts of glycerine, 54 parts of Arabic gum and 110 parts of mixing of water, wherein, Japanese Stephania Root stem cell extract, pot marigold extraction
The percentage that thing, oriental cherry extract, the gross mass of Western peony extract and dandelion extract account for skin care quality is
8.55%.
The specific preparation method that skin care item are nourished in above-mentioned conditioning comprises the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
(2) pot marigold extract is prepared by the following：Pot marigold is obtained after high speed bead mill method broken wall using water extraction
To pot marigold extract；
Oriental cherry extract is prepared by the following：Oriental cherry is taken after ultrasonication, oriental cherry is obtained using ethanol extraction method
Extract；
Western peony extract is prepared by the following：Western peony is taken after the micro-nano method broken wall of low temperature, is used
Ethanol extraction method obtains Western peony extract；
Dandelion extract is prepared by the following：Dandelion stem is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains dandelion extract.
(3) by pot marigold extract, oriental cherry extract, Western peony extract and dandelion extract and saualane 40
Part, 25 parts of glycerine, 54 parts of Arabic gum and 110 parts of mixing of water, after 36.8 degrees Celsius stir, it is unnecessary to be filtered to remove
Moisture, lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of conditioning nourishing face cream.
Embodiment 5
A kind of a kind of embodiment of conditioning tonic composition in the present invention, the composition contain following parts by weight into
Point：3.1 parts of Japanese Stephania Root stem cell extract, 0.6 part of pot marigold extract, 7.8 parts of oriental cherry extract, Western peony extract
1.1 parts and 9.5 parts of dandelion extract.
A kind of embodiment of skin care item is nourished in conditioning in the present invention, and the skin care item include above-mentioned composition, saualane 41
Part, 19 parts of glycerine, 45 parts of Arabic gum and 91 parts of mixing of water, wherein, Japanese Stephania Root stem cell extract, pot marigold extraction
The percentage that thing, oriental cherry extract, the gross mass of Western peony extract and dandelion extract account for skin care quality is
11.2%.
The specific preparation method that skin care item are nourished in above-mentioned conditioning comprises the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
(2) pot marigold extract is prepared by the following：Pot marigold is obtained after high speed bead mill method broken wall using water extraction
To pot marigold extract；
Oriental cherry extract is prepared by the following：Oriental cherry is taken after ultrasonication, oriental cherry is obtained using ethanol extraction method
Extract；
Western peony extract is prepared by the following：Western peony is taken after the micro-nano method broken wall of low temperature, is used
Methanol extraction obtains Western peony extract；
Dandelion extract is prepared by the following：Dandelion stem is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains dandelion extract.
(3) by pot marigold extract, oriental cherry extract, Western peony extract and dandelion extract and saualane 41
Part, 19 parts of glycerine, 45 parts of Arabic gum and 91 parts of mixing of water, after 37 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition Japanese Stephania Root stem cell extract, which is uniformly dispersed, produces a kind of conditioning nourishing face cream.
The antioxidant effect test of the skin care item of the present invention of embodiment 6
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, the shield of control group 1~4
Differ only in Japanese Stephania Root stem cell extract, oriental cherry extract and the Western Chinese herbaceous peony of skin frost and the face cream of embodiment 3 are extracted
The mass ratio of thing is different, and its preparation method is same as Example 3；The Japanese Stephania Root stem cell extraction of the face cream of control group 1~4
The mass ratio of thing, oriental cherry extract and Western peony extract is followed successively by：0.08：2.3：3.6；0.17：2.3：3.9；0.9：
2.3：5.2；1.4：2.3：5.9；
Method of testing：ORAC methods；Specifically, the face cream of Example 1~5 and 1~4 skin care as a control group respectively
Frost, it is configured to the aqueous solution, it is desirable to which extract total concentration is identical in the face cream aqueous solution, such as 1.6g/L, is used respectively using preceding
85mmol/L phosphate buffers (pH=7.2) dilute 1000 times and are used as test sample solution.
Test result：It is as shown in table 1 below；
The oxidation resistance test result of the face cream of the present invention of table 1
| Face cream | ORAC values | Face cream | ORAC values |
| Embodiment 1 | 698 | Control group 1 | 922 |
| Embodiment 2 | 1152 | Control group 2 | 1174 |
| Embodiment 3 | 1485 | Control group 3 | 1063 |
| Embodiment 4 | 877 | Control group 4 | 971 |
| Embodiment 5 | 854 |
Interpretation of result：It was found from upper table 1, the ORAC values of the face cream of embodiment 2,3 and control group 1~4 are higher, represent
The oxidation resistance of the face cream of embodiment 2 and 3 and control group 1~4 is preferable；Wherein, with the antioxygen of the face cream of embodiment 3
Change ability is optimal.
The fungistatic effect test of the skin care item of the present invention of embodiment 7
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, the shield of control group 1~4
The mass ratio for differing only in Japanese Stephania Root stem cell extract and pot marigold extract of skin frost and the face cream of embodiment 3 is not
Together, its preparation method is same as Example 3；Japanese Stephania Root stem cell extract and pot marigold extraction in the face cream of control group 1~4
The mass ratio of thing is 0.12 successively：3.7；0.56：3.7；0.79：3.7；0.79：3.7；1.1：3.7；
Method of testing：Choose staphylococcus, streptococcus, bacterium acidi propionici and corynebacteria, and LB solid mediums (i.e. LB
Flat board), face cream is dissolved into the aqueous solution that mass percent is 2.8% with water, by staphylococcus, streptococcus, bacterium acidi propionici
With corynebacteria according to quantity 1：1：1：Added after 1 mixing in the aqueous solution of face cream and form mixed solution, bacterium in mixed solution
Concentration be 1.2 × 106It is individual, drip 6 drop mixed solutions on LB flat boards with dropper respectively, the straight of bacterial plaque is measured after 60 hours
Footpath, and calculate bacterial plaque average diameter.
Test result：It is as shown in table 2 below；
The fungistatic effect of the face cream of the present invention of table 2
| Face cream | Bacterial plaque diameter | Face cream | Bacterial plaque diameter |
| Embodiment 1 | 0.42cm | Control group 1 | 0.33cm |
| Embodiment 2 | 0.22cm | Control group 2 | 0.19cm |
| Embodiment 3 | 0.01cm | Control group 3 | 0.26cm |
| Embodiment 4 | 0.39cm | Control group 4 | 0.35cm |
| Embodiment 5 | 0.65cm |
Interpretation of result：As shown in upper table 2, it is known that the face cream of embodiment 3, control group 1~4 and embodiment 2
Antibacterial effect is preferable, wherein, using the antibacterial ability of the face cream of embodiment 3 to be best.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. one kind conditioning tonic composition, it is characterised in that the composition contains the composition of following parts by weight：Japanese Stephania Root is dry thin
0.05~3.1 part of born of the same parents' extract, 0.6~7.3 part of pot marigold extract, 0.9~7.8 part of oriental cherry extract, the extraction of Western Chinese herbaceous peony
1.3~9.5 parts of 1.1~8.4 parts of thing and dandelion extract.
2. composition according to claim 1, it is characterised in that the Japanese Stephania Root stem cell extract, oriental cherry extract
Mass ratio with Western peony extract is 0.08~1.4：2.3：3.6~5.9.
3. composition according to claim 2, it is characterised in that the Japanese Stephania Root stem cell extract, oriental cherry extract
Mass ratio with Western peony extract is 0.4：2.3：4.5.
4. composition according to claim 1, it is characterised in that the Japanese Stephania Root stem cell extract and pot marigold extraction
The mass ratio of thing is 0.12~1.1：3.7.
5. composition according to claim 4, it is characterised in that the Japanese Stephania Root stem cell extract and pot marigold extraction
The mass ratio of thing is 0.33：3.7.
6. composition according to claim 1, it is characterised in that the composition contains the composition of following parts by weight：Thousand
Golden 0.4 part of rattan stem cell extract, 4.5 parts of pot marigold extract, 2.3 parts of oriental cherry extract, 4.5 parts of Western peony extract and
7.2 parts of dandelion extract.
7. skin care item are nourished in one kind conditioning, it is characterised in that the skin care item are included as described in any one of claim 1~6
Composition.
8. skin care item according to claim 7, it is characterised in that Japanese Stephania Root stem cell extract, gold in the skin care item
Small cup flower extract, oriental cherry extract, the quality percentage total amount of Western peony extract and dandelion extract for 8.55~
11.2%.
9. skin care item according to claim 7, it is characterised in that the Japanese Stephania Root stem cell extract is by the following method
Obtain：
S1, Japanese Stephania Root new life root or branch sterilization, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain described Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
10. skin care item according to claim 7, it is characterised in that the pot marigold extract is prepared by the following：
Pot marigold obtains pot marigold extract after high speed bead mill method broken wall using water extraction；
The oriental cherry extract is prepared by the following：Oriental cherry is taken after ultrasonication, oriental cherry extraction is obtained using alcohol extracting method
Thing；
The Western peony extract is prepared by the following：Western peony is taken after the micro-nano method broken wall of low temperature, is used
Alcohol extracting method obtains Western peony extract；
The dandelion extract is prepared by the following：Dandelion stem is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains dandelion extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711263850.0A CN107789310A (en) | 2017-12-04 | 2017-12-04 | Skin care item are nourished in a kind of conditioning containing Japanese Stephania Root stem cell extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711263850.0A CN107789310A (en) | 2017-12-04 | 2017-12-04 | Skin care item are nourished in a kind of conditioning containing Japanese Stephania Root stem cell extract |
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|---|---|---|---|
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1352640A1 (en) * | 2000-12-15 | 2003-10-15 | Kabushiki Kaisha Yakult Honsha | Compositions for retarding skin aging |
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
-
2017
- 2017-12-04 CN CN201711263850.0A patent/CN107789310A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1352640A1 (en) * | 2000-12-15 | 2003-10-15 | Kabushiki Kaisha Yakult Honsha | Compositions for retarding skin aging |
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (1)
| Title |
|---|
| 王建新 等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
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