CN107810927A - A kind of method for promoting black soldier flies mate and oviposit - Google Patents
A kind of method for promoting black soldier flies mate and oviposit Download PDFInfo
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- CN107810927A CN107810927A CN201711106501.8A CN201711106501A CN107810927A CN 107810927 A CN107810927 A CN 107810927A CN 201711106501 A CN201711106501 A CN 201711106501A CN 107810927 A CN107810927 A CN 107810927A
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- black soldier
- soldier flies
- adult
- ratio
- oviposit
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- 241001481656 Stratiomyidae Species 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000001737 promoting effect Effects 0.000 title claims abstract description 14
- 238000012258 culturing Methods 0.000 claims abstract description 18
- 238000005286 illumination Methods 0.000 claims abstract description 12
- 230000032669 eclosion Effects 0.000 claims abstract description 8
- 235000013601 eggs Nutrition 0.000 description 13
- 230000013011 mating Effects 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000012447 hatching Effects 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000709785 Hermetia illucens Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000255632 Tabanus atratus Species 0.000 description 1
- AEEAZFQPYUMBPY-UHFFFAOYSA-N [I].[W] Chemical compound [I].[W] AEEAZFQPYUMBPY-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000009318 large scale farming Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of method for promoting black soldier flies mate and oviposit, comprise the following steps:(1) black soldier flies adult is launched according to 2,000 8000 pairs every cubic metre of ratio in culturing chamber;(2) after adult eclosion, daytime is cultivating indoor green glow and gold-tinted mixing light source progress light filling, and intensity of illumination is 200 μm of ol.m at the 60cm of light source 40‑2.s‑1‑320μmol.m‑2.s‑1, 25 days light filling time.Because black soldier flies adult has extremely strong Preference to green glow and gold-tinted, black soldier flies adult is extremely active under the irradiation of both color of light, ceaselessly dance in the air, black soldier flies adult mate and oviposit can be promoted by using green glow and gold-tinted mixing light source progress light filling and improve the ratio of oosperm block;Additionally by the stocking density in adjustment intensity of illumination and culturing chamber, further promote black soldier flies mate and oviposit, improve black soldier flies female adult spawning ratio and oosperm block ratio.
Description
Technical field
The invention belongs to cultured insect field, and in particular to a kind of method for promoting black soldier flies adult mate and oviposit.
Background technology
Black soldier flies (Hermetia illucens L.) are a kind of worldwide widely distributed Diptera Stratiomyidae insects.It is black
4 development experience ovum, larva, pupa and adult worm states, fertility are strong from generation to generation for stratiomyiid.After adult eclosion, female adult pest starts for 1-2 days
Mating, starts to lay eggs for 3-4 days, about 9 days life-spans.Its larva saprophagous character, available for organic wastes such as bioconversion excrement, kitchen garbages
Gurry, and harmful microbe diversity reduces after processing.Contain abundant protein, ammonia inside the larva and prepupa of harvest
Base acid, fat etc., can be as the good feed of poultry, domestic animal and fish culture.As a kind of developable resource insect and ring
Border insect, the large-scale farming technology of black soldier flies still suffer from defect, and especially reproduction technique urgently improves.
Black soldier flies are day row caste, and under conditions of humiture is suitable, illumination especially sunlight is to limit its mating
The important factor of activity, current cultivation place is all to obtain sunlight, so as to promote adult to hand over using transparent warmhouse booth
With spawning.Light intensity can influence the mating of black soldier flies adult, and in the sun, Sheppard et al. is had found when light intensity is more than 200 μ
mol.m-2.s-1When, mating rate rise;And light intensity is less than 63 μm of ol.m-2.s-1When, mating activity does not occur, and think Heisui River
Horsefly only can just mate in the sun.In spite of sunlight, the spawning rate of female adult pest is less than 40%, and only 39.5% ovum
Block is oosperm block.Although iodine-tungsten lamp can overcome the disadvantages that the problem of sunlight deficiency rainy days, mating rate only has 61% under full sunlight,
And more than 110 μm ol.m of light intensity-2.s-1When, mating activity reduces.When having tried to cultivate black soldier flies in recent years indoors simultaneously
Using more fluorescent lamps and LED, to promote adult to mate, but result finds that the spawning rate of female adult only has 16%, and embryonated egg
The ratio of block only 11.2%.
The content of the invention
It is an object of the invention to overcome above-mentioned technical deficiency, a kind of method for promoting black soldier flies mate and oviposit, solution are proposed
The certainly low technical problem low with oosperm block ratio of black soldier flies female adult spawning ratio in the prior art.
To reach above-mentioned technical purpose, technical scheme provides a kind of method for promoting black soldier flies mate and oviposit,
It is as follows including step:
S1 launches black soldier flies adult in culturing chamber according to every cubic metre 2000-8000 pairs of ratio;
S2 is after adult eclosion, and daytime is cultivating indoor green glow and gold-tinted mixing light source progress light filling, apart from light source 40-
Intensity of illumination is 200 μm of ol.m at 60cm-2.s-1-μmol.m-2.s-1, 2-5 days light filling time.
Compared with prior art, beneficial effects of the present invention include:
The method for the promotion black soldier flies mate and oviposit that the technical program provides, is existed by using green glow and gold-tinted mixing light source
Light filling is carried out in culturing chamber, black soldier flies adult has extremely strong Preference to green glow and gold-tinted, in the irradiation of both color of light
Lower black soldier flies adult is extremely active, ceaselessly dances in the air, and can promote black soldier flies adult mate and oviposit and improve the ratio of oosperm block
Example;Intensity of illumination is to influence a key factor of black soldier flies adult mating ratio, and intensity of illumination is in 200 μm of ol.m-2.s-1-
320μmol.m-2.s-1In the range of, black soldier flies adult mating ratio can be improved;If the stocking density in culturing chamber is too small, support
Growing room cannot sufficiently utilize, and can cause the waste of resource, if the stocking density in culturing chamber is too big, can cause indoor lack
Oxygen and black soldier flies adult activity space are limited, so black according to every cubic metre 2000-8000 pairs of quantity dispensing into culturing chamber
Stratiomyiid adult, female adult spawning ratio can be improved.The method of the promotion black soldier flies mate and oviposit provided using the technical program, can
To promote black soldier flies mate and oviposit, black soldier flies female adult spawning ratio and oosperm block ratio are improved.
Brief description of the drawings
Fig. 1 is the embodiment of the present invention 1 and comparative example 1 ratio that female adult is laid eggs under different stocking densities;
Fig. 2 is the ratio of the embodiment of the present invention 1 and comparative example 1 oosperm block under different stocking densities.
Embodiment
The present embodiment provides a kind of method for promoting black soldier flies mate and oviposit, specifically, comprises the following steps:
S1 launches black soldier flies adult in culturing chamber according to every cubic metre 2000-8000 pairs of ratio;
S2 is after adult eclosion, and daytime is cultivating indoor green glow and gold-tinted mixing light source progress light filling, apart from light source 40-
Intensity of illumination is 200 μm of ol.m at 60cm-2.s-1-320μmol.m-2.s-1, 2-5 days light filling time;Preferably, green glow and Huang
The ratio of green glow and gold-tinted is 1 in light mixing light source:1.
Specifically, the green wavelength in the step S2 is 530-560nm, and peak value is 545 ± 10nm.
Specifically, the yellow wavelengths scope in the step S2 is 580-620nm, and peak value is 610 ± 10nm.
Specifically, the daytime in the step S2 is 7:00-20:00, with 220v alternating currents driving power and power switch
Using timing control apparatus, in daily 7:00 switches on power, and 20:00 deenergization.
The method of black soldier flies mate and oviposit is promoted to be further described the present invention below in conjunction with specific embodiment.Under
The embodiment of face description is exemplary, is only used for explaining the present invention, and is not considered as limiting the invention.
Light source preferably carries out light filling using green LED and yellow light-emitting diode in following embodiments, and wherein
Green wavelength is 530-560nm, and peak value is 545 ± 10nm, and yellow wavelengths scope is 580-620nm, peak value is 610 ±
10nm;Because part black soldier flies adult can fly to light source, in order to prevent that fluorescent tube is dirty, by green LED and Yellow luminous
Diode is placed in lampshade and used.
Embodiment 1
The present embodiment provides a kind of method for promoting black soldier flies mate and oviposit, specifically comprises the following steps:
1. the light emitting diode of green glow and gold-tinted can be simultaneously emitted by cultivation indoor location, electric drive green glow is exchanged with 220v
With the light emitting diode of gold-tinted, and with timing control apparatus control power supply switch, be arranged on 7:00 switches on power, and 20:00 is disconnected
Switch power supply;Then intensity of illumination is 300 μm of ol.m at adjustable range light source 50cm-2.s-1, deenergization after setting.
2. launch black soldier flies adult according to 4000 pairs every cubic metre of ratio in culturing chamber.
3. after adult eclosion, switch on power, daytime indoor can be simultaneously emitted by luminous the two of green glow and gold-tinted cultivating
Pole pipe carries out light filling, is irradiated 5 days with light emitting diode.
Collect pieces of an egg and carry out hatching experiment, the ratio for obtaining female adult spawning is 59.8% and the ratio of oosperm block is
83%.
Embodiment 2
The present embodiment provides a kind of method for promoting black soldier flies mate and oviposit, specifically comprises the following steps:
1. according to 1 in culturing chamber:1 ratio installation green LED and yellow light-emitting diode, is handed over 220v
Electric drive green LED and yellow light-emitting diode are flowed, and the switch of power supply is controlled with timing control apparatus, is arranged on
7:00 switches on power, and 20:00 deenergization;Then intensity of illumination is 320 μm of ol.m at adjustable range light source 40cm-2.s-1If
Deenergization after putting.
2. launch black soldier flies adult according to 2000 pairs every cubic metre of ratio in culturing chamber.
3. after adult eclosion, switch on power, daytime is cultivating indoor green glow and gold-tinted mixing light source progress light filling, uses
Green glow and gold-tinted mixing light source irradiate 3 days.
Collect pieces of an egg and carry out hatching experiment, the ratio for obtaining female adult spawning is 79% and the ratio of oosperm block is 82%.
Embodiment 3
The present embodiment provides a kind of method for promoting black soldier flies mate and oviposit, specifically comprises the following steps:
1. according to 1 in culturing chamber:1 ratio installation green LED and yellow light-emitting diode, is handed over 220v
Electric drive green LED and yellow light-emitting diode are flowed, and the switch of power supply is controlled with timing control apparatus, is arranged on
7:00 switches on power, and 20:00 deenergization;Then intensity of illumination is 200 μm of ol.m at adjustable range light source 60cm-2.s-1If
Deenergization after putting.
2. launch black soldier flies adult according to 8000 pairs every cubic metre of ratio in culturing chamber.
3. after adult eclosion, switch on power, daytime is cultivating indoor green glow and gold-tinted mixing light source progress light filling, uses
Green glow and gold-tinted mixing light source irradiate 2 days.
Collect pieces of an egg and carry out hatching experiment, the ratio for obtaining female adult spawning is 50% and the ratio of oosperm block is
80.5%.
In order to illustrate the effect of the present invention, it is as follows that inventor provides comparative experiments:
Comparative example 1
The quantity for the black soldier flies adult launched into culturing chamber is respectively according to every cubic metre 2000 pairs, 8000 pairs, 12000 pairs
Ratio with 16000 pairs is launched, and for remaining parameter with identical in example 1, the cultural method of use is also identical.
Collect the pieces of an egg in embodiment 1 and comparative example 1 under different stocking densities and carry out hatching experiment female adult production for confirmation
The ratio of ovum and the ratio for checking oosperm block.The ratiometric result that female adult is laid eggs under different stocking densities, as shown in Figure 1;It is different
The ratiometric result of oosperm block under stocking density, as shown in Figure 2.
As seen from Figure 1, when every cubic metre of interior is put into 2000 pairs of black soldier flies adults, its mating is promoted using this method
Spawning, the ratio of female adult spawning reach 80%;When indoor every cubic metre of adult quantity being put into reaches 8000 pairs, this method is used
Promote its mate and oviposit, the ratio of female adult spawning still has 51%.And indoor black soldier flies imago breeding density is bigger, female adult spawning
Ratio is smaller, and when launching 2000-8000 to black soldier flies adult according to every cubic metre in culturing chamber, the ratio of female adult spawning is higher than
50%;But when being launched in culturing chamber according to every cubic metre more than 12000 pairs of black soldier flies adults, the ratio of female adult spawning is less than
40%.As seen from Figure 2, under all stocking densities, the ratio of oosperm block is more than 80%.Illustrate to control in culturing chamber
Black soldier flies imago breeding density can significantly improve female adult spawning ratio.
Comparative example 2
The diode of the diode and a Yellow light-emitting low temperature that only send green glow is chosen respectively in the supplementary lighting sources of cultivation indoor location,
For remaining parameter with identical in example 1, the cultural method of use is also identical.
Collect the pieces of an egg after different colored light sources light filling is used in embodiment 1 and comparative example 2 and carry out hatching experiment so as to true
Recognize the ratio of female adult spawning and check the ratio of oosperm block.The ratio and fertilization that different colored light sources are laid eggs to black soldier flies female adult
The influence result of pieces of an egg, as shown in table 1.
The ratio and the influence of oosperm block that the different colored light sources of table 1 are laid eggs to black soldier flies female adult
Light source colour | The ratio of female adult spawning | The ratio of oosperm block |
Green+yellow | 59.8% | 83% |
Green | 46% | 41.6% |
Yellow | 45% | 42.3% |
As can be seen from Table 1, light filling is carried out using the light emitting diode that can be simultaneously emitted by green glow and gold-tinted, compared to green
Light monochromatic light or yellow color light carry out light filling, can improve the ratio of female adult spawning and the ratio of oosperm block, especially may be used
Significantly to improve the ratio of oosperm block.
The ratio that can draw every cubic metre 2000-8000 pairs of control by embodiment 1-3, comparative example 1 and comparative example 2 is launched
Black soldier flies adult, adjustment intensity of illumination are 200 μm of ol.m-2.s-1-320μmol.m-2.s-1, and with green glow and gold-tinted mixing light source
Carry out light filling 2-5 days, the spawning ratio of female adult can be caused to be not less than 50%, and the ratio of oosperm block is higher than 80%;Compare
The spawning rate of female adult pest is less than 40% under sunlight, and the ratio of oosperm block increases significantly for 39.5%.
The inventive method can promote black soldier flies adult mate and oviposit, and make the spawning ratio of female adult not less than 50%, and by
The ratio of smart pieces of an egg reaches more than 80%.The inventive method can provide a large amount of oosperm blocks in raising black soldier flies on a large scale,
Possess the potentiality promoted the use of a large area, and to being widely popularized the black soldier flies converting animal-poultry faeces utilized and kitchen garbage at present
Pinch technology is that the improvement of black soldier flies raising technology has important directive significance.
Finally, method of the invention is only preferable embodiment, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements made etc., the protection of the present invention should be included in
Within the scope of.
Claims (4)
- A kind of 1. method for promoting black soldier flies mate and oviposit, it is characterised in that comprise the following steps:S1 launches black soldier flies adult in culturing chamber according to every cubic metre 2000-8000 pairs of ratio;S2 is after adult eclosion, and daytime is cultivating indoor green glow and gold-tinted mixing light source progress light filling, apart from light source 40-60cm Place's intensity of illumination is 200 μm of ol.m-2.s-1-320μmol.m-2.s-1, 2-5 days light filling time.
- 2. a kind of method for promoting black soldier flies mate and oviposit according to claim 1, it is characterised in that in the step S2 Green wavelength is 530-560nm, and peak value is 545 ± 10nm.
- 3. a kind of method for promoting black soldier flies mate and oviposit according to claim 1, it is characterised in that in the step S2 Yellow wavelengths scope is 580-620nm, and peak value is 610 ± 10nm.
- 4. a kind of method for promoting black soldier flies mate and oviposit according to claim 1, it is characterised in that in the step S2 Daytime is 7:00-20:00.
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CN111066729A (en) * | 2018-10-19 | 2020-04-28 | 广州安芮洁环保科技有限公司 | Method for improving egg laying efficiency of hermetia illucens adults |
CN115076623A (en) * | 2022-06-28 | 2022-09-20 | 江苏省农业科学院 | LED light source for breeding adult hermetia illucens and breeding method of adult hermetia illucens |
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CN115076623A (en) * | 2022-06-28 | 2022-09-20 | 江苏省农业科学院 | LED light source for breeding adult hermetia illucens and breeding method of adult hermetia illucens |
CN115076623B (en) * | 2022-06-28 | 2023-11-14 | 江苏省农业科学院 | LED light source for adult black soldier flies and adult black soldier flies breeding method |
WO2024000611A1 (en) * | 2022-06-28 | 2024-01-04 | 江苏省农业科学院 | Led light source for adult hermetia illucens breeding and adult hermetia illucens breeding method |
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