CN107810851A - A kind of iris Initial culture base - Google Patents
A kind of iris Initial culture base Download PDFInfo
- Publication number
- CN107810851A CN107810851A CN201711104091.3A CN201711104091A CN107810851A CN 107810851 A CN107810851 A CN 107810851A CN 201711104091 A CN201711104091 A CN 201711104091A CN 107810851 A CN107810851 A CN 107810851A
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- China
- Prior art keywords
- iris
- initial culture
- culture base
- present
- iris initial
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of iris Initial culture base, including：BA+ sucrose+35g/L+ agar the 5g/L of MS+0.2mg/L~0.5mg/L GA3+2~3mg/L 6.Compared with prior art, the beneficial effects of the invention are as follows：The iris Initial culture base of the present invention, it is simple to operate, cost is cheap, the quick breedings of iris are viewed and admired suitable for majority.
Description
Technical field
The invention belongs to field of plant tissue culture technique, and in particular to a kind of iris Initial culture base.
Background technology
Iris (Phalaenopsis aphrodite Rchb.F.) is orchid family Phalaenopsis, originates in hylaeion hypotropicum
Area, for growing nonparasitically upon another plant property orchid.The thick aerial root of iris white is exposed at around blade, except the work with nutrient in absorption air
With outer, also growth and photosynthesis.In the time in the new year, Phalaenopsis plants extract long bennet out from axil, and output shape
Flower as butterfly dances in the air, the deep favor by flower fans, it is known as the title of " cattleya queen consort ".
In order to meet the needs of flowers city industry, traditional iris planting patterns has been difficult to meet the market demand, because
The most frequently used implantation methods of this current iris be using its tissue carry out vegetative propagation, so can either amount reproduction plant,
And can enough makes filial generation iris meet maternal phenotypic character, and application prospect is good.
At present, in the rapid propagation in vitro research of iris, the Initial culture of sterile system is a big difficult point.
The content of the invention
A kind of iris Initial culture base provided by the invention, in south of the Five Ridges Guangdong and Guangxi Provinces area, for iris difference growth step
It the different culture medium of Demand Design of section, can shorten whole iris growth cycle by this method.
A kind of iris Initial culture base provided by the invention, including：
MS+0.2mg/L~0.5mg/L GA3+2~3mg/L 6-BA+ sucrose+35g/L+ agar 5g/L.
Further, in addition to：
1mg/L~3mg/L TDZ.
Further, the sucrose is 20-35g/L.
Thidiazuron TDZ is colorless and odorless crystal.TDZ is a kind of new plant growth regulator, has very strong cell point
Split plain active (CTK), tens times higher than general CTK of its CTK activity to hundred times, research shows：It can promote plant
The regeneration and breeding of bud, that breaks bud stops eye, promotes seed to sprout, promotes callus growth, delay plant senescence etc..And
The growth and development process of plant can be adjusted to the effect of other plant hormones and physiological activator, be an active force
Very strong plant growth regulator.Agriculturally it is being widely used and promotional value.
Compared with prior art, the beneficial effects of the invention are as follows：
The iris Initial culture base of the present invention, it is simple to operate, cost is cheap, view and admire the quick numerous of iris suitable for majority
Grow.
Embodiment
With reference to specific embodiment, the present invention is described in detail, but should not be construed as the limitation of the present invention.Below
The experimental method of unreceipted actual conditions in embodiment, carried out according to the conventional method and condition of this area.
A kind of iris Initial culture base provided by the invention, including：
MS+0.2mg/L~0.5mg/L GA3+2~3mg/L 6-BA+ sucrose+35g/L+ agar 5g/L.
As the improvement of specific embodiment, in addition to：
1mg/L~3mg/L TDZ.
As the improvement of specific embodiment, the sucrose is 20-35g/L.
It is above-mentioned standby after the autoclave sterilization of routine.
Original cuiture is that the part that tissue or organ are directly obtained from organism is cultivated, also referred to as Initial culture.Sternly
Say i.e. from internal tissue inoculated and cultured of taking out to passage stage first time, but in fact, generally the first generation to the tenth generation lattice
Within culture cell be referred to as primitive cell culture.Typically continue 1 one 4 weeks.This phase cell is in active movement, it is seen that cell
Division, but it is not vigorous.The similitude on morphosis and functional activity is big with internal basic stitch for primary cultured cell.Due to culture
Cell just separated from biological tissue, therefore closer to the animation in organism.This method can be postgraduate
Growth, metabolism, the breeding of object cell provide strong means.Also created conditions simultaneously for later Secondary Culture.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included in the interest field that the present invention is limited within protection scope of the present invention.
Claims (3)
- A kind of 1. iris Initial culture base, it is characterised in that including：MS+0.2mg/L~0.5mg/L GA3+2~3mg/L 6-BA+ sucrose+35g/L+ agar 5g/L.
- 2. a kind of iris Initial culture base according to claim 1, it is characterised in that also include：1mg/L~3mg/L TDZ.
- 3. a kind of iris Initial culture base according to claim 1, it is characterised in that the sucrose is 20-35g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711104091.3A CN107810851A (en) | 2017-11-10 | 2017-11-10 | A kind of iris Initial culture base |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711104091.3A CN107810851A (en) | 2017-11-10 | 2017-11-10 | A kind of iris Initial culture base |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107810851A true CN107810851A (en) | 2018-03-20 |
Family
ID=61608888
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711104091.3A Pending CN107810851A (en) | 2017-11-10 | 2017-11-10 | A kind of iris Initial culture base |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107810851A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4684612A (en) * | 1984-07-27 | 1987-08-04 | Sungene Technologies Corporation | Process for regenerating soybeans |
| CN104429950A (en) * | 2014-11-14 | 2015-03-25 | 佛山市顺德区今日景艺生物科技有限公司 | Phalaenopsis blume culture medium and culture method |
| CN104686362A (en) * | 2015-03-24 | 2015-06-10 | 芜湖东源新农村开发股份有限公司 | Butterfly orchid tissue culturing method |
| CN106106163A (en) * | 2016-07-12 | 2016-11-16 | 成都东山兰韵农业有限公司 | A kind of iris cultivates propagation method |
-
2017
- 2017-11-10 CN CN201711104091.3A patent/CN107810851A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4684612A (en) * | 1984-07-27 | 1987-08-04 | Sungene Technologies Corporation | Process for regenerating soybeans |
| CN104429950A (en) * | 2014-11-14 | 2015-03-25 | 佛山市顺德区今日景艺生物科技有限公司 | Phalaenopsis blume culture medium and culture method |
| CN104686362A (en) * | 2015-03-24 | 2015-06-10 | 芜湖东源新农村开发股份有限公司 | Butterfly orchid tissue culturing method |
| CN106106163A (en) * | 2016-07-12 | 2016-11-16 | 成都东山兰韵农业有限公司 | A kind of iris cultivates propagation method |
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Application publication date: 20180320 |
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