CN107691221A - A kind of iris tissue culture method - Google Patents
A kind of iris tissue culture method Download PDFInfo
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- CN107691221A CN107691221A CN201711104509.0A CN201711104509A CN107691221A CN 107691221 A CN107691221 A CN 107691221A CN 201711104509 A CN201711104509 A CN 201711104509A CN 107691221 A CN107691221 A CN 107691221A
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- iris
- culture
- stem apex
- illumination
- sterilization
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of iris tissue culture method, including iris stem apex obtains, iris stem apex sterilization and carries out Initial culture, squamous subculture and culture of rootage to the iris stem apex after sterilization.Beneficial effects of the present invention：(1) it is up to 20% by the iris tissue-cultured seedling plant of the present invention, rooting rate.(2) merit of original kind can be kept using tissue-cultured seedling caused by stem apex progress cultured in vitro, is laid a good foundation for stock breeding and industrialization production.(3) iris tissue culture method of the invention, it is simple to operate, cost is cheap, the quick breedings of iris are viewed and admired suitable for majority.
Description
Technical field
The invention belongs to field of plant tissue culture technique, and in particular to a kind of iris tissue culture method.
Background technology
Iris (Phalaenopsis aphrodite Rchb.F.) is orchid family Phalaenopsis, originates in hylaeion hypotropicum
Area, for growing nonparasitically upon another plant property orchid.The thick aerial root of iris white is exposed at around blade, except the work with nutrient in absorption air
With outer, also growth and photosynthesis.In the time in the new year, Phalaenopsis plants extract long bennet out from axil, and output shape
Flower as butterfly dances in the air, the deep favor by flower fans, it is known as the title of " cattleya queen consort ".
In order to meet the needs of flowers city industry, traditional iris planting patterns has been difficult to meet the market demand, because
The most frequently used implantation methods of this current iris be using its tissue carry out vegetative propagation, so can either amount reproduction plant,
It can be that filial generation iris meets maternal phenotypic character to have, and application prospect is good.
At present, the rapid propagation in vitro research of Phalaenopsis plant focuses mostly on tree peony, the state about iris tissue cultures
Inside and outside research is less, existing on iris tissue culture technique, the foundation of sterile system is only discussed, also without formation one
The whole series utilize iris underground bud rapid propagation method, are also not involved with taking root and transplanting for iris tissue-cultured seedling, it is impossible to
Foundation is provided for production.
The content of the invention
A kind of iris tissue culture method provided by the invention, in south of the Five Ridges Guangdong and Guangxi Provinces area, grown for iris is different
It the different culture medium of the Demand Design in stage, can shorten whole iris growth cycle by this method.
A kind of iris tissue culture method provided by the invention, including iris stem apex obtains, the sterilizing of iris stem apex
The step of sterilizing and Initial culture, squamous subculture and culture of rootage carried out to the iris stem apex after sterilization：
(1) iris stem apex obtains：The Lanzhou-Xinjiang budlet sprouted of autumn clip butterfly, after pure water flow wash 35min
It is put on superclean bench；
(2) iris stem apex sterilization：With alcohol-pickled 20~29 seconds of 75%, then 30 are soaked with 2% mercuric chloride
~60 seconds, and blot surface moisture；
(3) Initial culture of iris stem apex：Culture 20-25 days, temperature is 23-27 DEG C, illumination 12H, intensity of illumination
1500-2000Lux；
The culture medium of described Initial culture is MS+0.2mg/L~0.5mg/L GA3+2~3mg/L 6-BA；
(4) squamous subculture of iris：
Budlet base portion is taken, is cut into the segment of lcm length, is inoculated on the MS culture mediums that pH value is 6.3-6.8；
Multiplying culture 4 weeks, the cultivation temperature of Multiplying culture is 23-27 DEG C, illumination 12H, intensity of illumination 1500-2000Lux；
The culture medium of described squamous subculture is MS+1.5mg/L~2mg/L 6-BA+1mg/L~2mg/L TDZ, sucrose
35g/L, agar 5g/L,
(5) culture of rootage of iris：
Root media is adds the MS culture mediums of activated carbon, when root long is 2-4cm, carries out rooting culture, will cultivate
Bottle moves to hardening 15-20 days in greenhouse, washes away culture medium, is transplanted into hole tray, cover film, while is removed after shade 75%, 2 weeks
Film is removed, gives full exposure, carries out rich water quality management.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) it is up to 20% by the iris tissue-cultured seedling plant of the present invention, rooting rate.
(2) merit of original kind can be kept using tissue-cultured seedling caused by stem apex progress cultured in vitro, is that breeding is numerous
Educate and laid a good foundation with industrialization production.
(3) iris tissue culture method of the invention, it is simple to operate, cost is cheap, view and admire iris suitable for majority
Quick breeding.
Embodiment
With reference to specific embodiment, the present invention is described in detail, but should not be construed as the limitation of the present invention.Below
The experimental method of unreceipted actual conditions in embodiment, carried out according to the conventional method and condition of this area.
Embodiment 1
(1) iris stem apex obtains：The Lanzhou-Xinjiang budlet sprouted of autumn clip butterfly, after pure water flow wash 35min
It is put on superclean bench；
(2) iris stem apex sterilization：With alcohol-pickled 20~29 seconds of 75%, then 30 are soaked with 2% mercuric chloride
~60 seconds, and blot surface moisture；
(3) Initial culture of iris stem apex：Culture 20-25 days, temperature is 23-27 DEG C, illumination 12H, intensity of illumination
1500-2000Lux；
The culture medium of described Initial culture is MS+0.2mg/L~0.5mg/L GA3+2~3mg/L 6-BA；
(4) squamous subculture of iris：
Budlet base portion is taken, is cut into the segment of lcm length, is inoculated on the MS culture mediums that pH value is 6.3-6.8；
Multiplying culture 4 weeks, the cultivation temperature of Multiplying culture is 23-27 DEG C, illumination 12H, intensity of illumination 1500-2000Lux；
The culture medium of described squamous subculture is MS+1.5mg/L~2mg/L 6-BA+1mg/L~2mg/L TDZ, sucrose
35g/L, agar 5g/L,
(5) culture of rootage of iris：
Root media is adds the MS culture mediums of activated carbon, when root long is 2-4cm, carries out rooting culture, will cultivate
Bottle moves to hardening 15-20 days in greenhouse, washes away culture medium, is transplanted into hole tray, cover film, while is removed after shade 75%, 2 weeks
Film is removed, gives full exposure, carries out rich water quality management.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included in the interest field that the present invention is limited within protection scope of the present invention.
Claims (2)
1. a kind of iris tissue culture method, it is characterised in that comprise the following steps：
S1. the acquisition of iris stem apex and sterilization
Iris stem apex obtains：The Lanzhou-Xinjiang budlet sprouted of autumn clip butterfly, is put in ultra-clean after pure water flow wash 35min
On workbench；
Iris stem apex sterilization：With alcohol-pickled 20~29 seconds of 75%, then soaked 30~60 seconds with 2% mercuric chloride,
And blot surface moisture；
S2. the Initial culture of iris stem apex：Culture 20-25 days, temperature is 23-27 DEG C, illumination 12H, intensity of illumination 1500-
2000Lux；
The culture medium of described Initial culture is MS+0.2mg/L~0.5mg/L GA3+2~3mg/L 6-BA；
S3. the squamous subculture of iris：
Budlet base portion is taken, is cut into the segment of lcm length, is inoculated on the MS culture mediums that pH value is 6.3-6.8；
Multiplying culture 4 weeks, the cultivation temperature of Multiplying culture is 23-27 DEG C, illumination 12H, intensity of illumination 1500-2000Lux；
The culture medium of described squamous subculture is MS+1.5mg/L~2mg/L 6-BA+1mg/L~2mg/L TDZ, sucrose 35g/
L, agar 5g/L.
2. a kind of iris tissue culture method according to claim 1, it is characterised in that further comprising the steps of：
The culture of rootage of iris, root media, when root long is 2-4cm, are tamed and dociled to add the MS culture mediums of activated carbon
Change transplanting, blake bottle is moved into hardening 15-20 days in greenhouse, culture medium is washed away, is transplanted into hole tray, cover film, hide simultaneously
Film is removed after shade 75%, 2 weeks, gives full exposure, carries out rich water quality management.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711104509.0A CN107691221A (en) | 2017-11-10 | 2017-11-10 | A kind of iris tissue culture method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711104509.0A CN107691221A (en) | 2017-11-10 | 2017-11-10 | A kind of iris tissue culture method |
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| Publication Number | Publication Date |
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| CN107691221A true CN107691221A (en) | 2018-02-16 |
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| CN201711104509.0A Pending CN107691221A (en) | 2017-11-10 | 2017-11-10 | A kind of iris tissue culture method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618919A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | A kind of cultural method improving exhibition shape cherry iris flowering rate |
| CN109618918A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | Open up the breeding method of shape cherry iris |
| CN110250002A (en) * | 2019-07-15 | 2019-09-20 | 上海师范大学 | The induction of iris protocorm and transgenic method |
| CN114532225A (en) * | 2022-02-28 | 2022-05-27 | 广西壮族自治区农业科学院 | Rapid propagation and cultivation method for paphiopedilum delavayi tissue culture |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4684612A (en) * | 1984-07-27 | 1987-08-04 | Sungene Technologies Corporation | Process for regenerating soybeans |
| CN104429950A (en) * | 2014-11-14 | 2015-03-25 | 佛山市顺德区今日景艺生物科技有限公司 | Phalaenopsis blume culture medium and culture method |
| CN104686362A (en) * | 2015-03-24 | 2015-06-10 | 芜湖东源新农村开发股份有限公司 | Butterfly orchid tissue culturing method |
| CN106106163A (en) * | 2016-07-12 | 2016-11-16 | 成都东山兰韵农业有限公司 | A kind of iris cultivates propagation method |
-
2017
- 2017-11-10 CN CN201711104509.0A patent/CN107691221A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4684612A (en) * | 1984-07-27 | 1987-08-04 | Sungene Technologies Corporation | Process for regenerating soybeans |
| CN104429950A (en) * | 2014-11-14 | 2015-03-25 | 佛山市顺德区今日景艺生物科技有限公司 | Phalaenopsis blume culture medium and culture method |
| CN104686362A (en) * | 2015-03-24 | 2015-06-10 | 芜湖东源新农村开发股份有限公司 | Butterfly orchid tissue culturing method |
| CN106106163A (en) * | 2016-07-12 | 2016-11-16 | 成都东山兰韵农业有限公司 | A kind of iris cultivates propagation method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618919A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | A kind of cultural method improving exhibition shape cherry iris flowering rate |
| CN109618918A (en) * | 2019-02-14 | 2019-04-16 | 芜湖东源新农村开发股份有限公司 | Open up the breeding method of shape cherry iris |
| CN110250002A (en) * | 2019-07-15 | 2019-09-20 | 上海师范大学 | The induction of iris protocorm and transgenic method |
| CN114532225A (en) * | 2022-02-28 | 2022-05-27 | 广西壮族自治区农业科学院 | Rapid propagation and cultivation method for paphiopedilum delavayi tissue culture |
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