CN107637524A - A kind of iris root media - Google Patents
A kind of iris root media Download PDFInfo
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- CN107637524A CN107637524A CN201711103652.8A CN201711103652A CN107637524A CN 107637524 A CN107637524 A CN 107637524A CN 201711103652 A CN201711103652 A CN 201711103652A CN 107637524 A CN107637524 A CN 107637524A
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- iris
- root media
- iris root
- plant
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Abstract
The present invention relates to a kind of iris root media, including the BA+ activated carbons of MS+0.5mg/L~1mg/L 6+sucrose+35g/L+ agar 5g/L.Compared with prior art, the beneficial effects of the invention are as follows：(1) it is up to 30% by the iris tissue-cultured seedling plant of the present invention, rooting rate.(2) iris root media of the invention, it is simple to operate, cost is cheap, the quick breedings of iris are viewed and admired suitable for majority.
Description
Technical field
The invention belongs to field of plant tissue culture technique, and in particular to a kind of iris root media.
Background technology
Iris (Phalaenopsis aphrodite Rchb.F.) is orchid family Phalaenopsis, originates in hylaeion hypotropicum
Area, for growing nonparasitically upon another plant property orchid.The thick aerial root of iris white is exposed at around blade, except the work with nutrient in absorption air
With outer, also growth and photosynthesis.In the time in the new year, Phalaenopsis plants extract long bennet out from axil, and output shape
Flower as butterfly dances in the air, the deep favor by flower fans, it is known as the title of " cattleya queen consort ".
In order to meet the needs of flowers city industry, traditional iris planting patterns has been difficult to meet the market demand, because
The most frequently used implantation methods of this current iris be using its tissue carry out vegetative propagation, so can either amount reproduction plant,
And can enough makes filial generation iris meet maternal phenotypic character, and application prospect is good.
At present, in the rapid propagation in vitro research of iris, taking root for sterile system is a big difficult point.
The content of the invention
A kind of iris root media provided by the invention, in south of the Five Ridges Guangdong and Guangxi Provinces area, for iris difference growth step
It the different culture medium of Demand Design of section, can shorten whole iris growth cycle by this method.
A kind of iris root media provided by the invention, including：
MS+0.5mg/L~1mg/L 6-BA+ activated carbons+sucrose+35g/L+ agar 5g/L.
Further, in addition to：
3mg/L~5mg/L TDZ.
Further, in addition to：
1mg/L~5mg/L PPP333.
Further, the activated carbon is 1-5g/L.
Further, the sucrose is 20-35g/L.
Thidiazuron TDZ is colorless and odorless crystal.TDZ is a kind of new plant growth regulator, has very strong cell point
Split plain active (CTK), tens times higher than general CTK of its CTK activity to hundred times, research shows：It can promote plant
The regeneration and breeding of bud, that breaks bud stops eye, promotes seed to sprout, promotes callus growth, delay plant senescence etc..And
The growth and development process of plant can be adjusted to the effect of other plant hormones and physiological activator, be an active force
Very strong plant growth regulator.Agriculturally it is being widely used and promotional value.
PPP333 paclobutrazols, it is the triazole type plant growth regulator succeeded in developing the eighties, is endogenous gibberellins synthesis
Inhibitor.Also the activity of rice indoleacetic acid oxidase can be improved, reduces the level of rice seedling Endogenous IAA.It is obvious to weaken rice seedling
Apical growth advantage, lateral bud (tiller) is promoted to grow.Rice shoot appearance is short to strengthen more tillers, and leaf color is dark green.Well developed root system.Anatomy
Research shows that paclobutrazol can make the cell of rice seedling root, leaf sheath, leaf diminish, the cell number of plies increase of each organ.Tracer analysis table
Bright, rice paddy seed, leaf, root can absorb paclobutrazol.Blade absorb paclobutrazol be largely trapped in absorb part, seldom to
Outer transport.Paclobutrazol low concentration promotes the photosynthetic efficiency of rice seedling blade；High concentration suppresses photosynthetic efficiency, and it is strong to improve root system respiration
Degree, aerial part respiratory intensity is reduced, improve Stoma of Leaves resistance, reduce leaf transpiration effect.
The agriculture application value of paclobutrazol is its control effect to plant growth.With plant growth is delayed, suppress
Cane extends, and shortens internode, promotes plant tillering, promotes bud differentiation, increases plant stress-resistance performance, improve yield and other effects.
This product is applied to water paddy and wheat class, peanut, fruit tree, tobacco, rape, soybean, flowers, lawn etc. and makees (plant) thing, and using effect shows
Write.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) it is up to 30% by the iris tissue-cultured seedling plant of the present invention, rooting rate.
(2) iris root media of the invention, it is simple to operate, cost is cheap, view and admire the fast of iris suitable for majority
Speed breeding.
Embodiment
With reference to specific embodiment, the present invention is described in detail, but should not be construed as the limitation of the present invention.Below
The experimental method of unreceipted actual conditions in embodiment, carried out according to the conventional method and condition of this area.
A kind of iris root media provided by the invention, including：
MS+0.5mg/L~1mg/L 6-BA+ activated carbons+sucrose+35g/L+ agar 5g/L.
As the improvement of specific embodiment, in addition to：
3mg/L~5mg/L TDZ.
As the improvement of specific embodiment, in addition to：
1mg/L~5mg/L PPP333.
As the improvement of specific embodiment, the activated carbon is 1-5g/L.
As the improvement of specific embodiment, the sucrose is 20-35g/L.
It is above-mentioned standby after the autoclave sterilization of routine.
When root long is 2-4cm, rooting culture is carried out, blake bottle is moved into hardening 15-20 days in greenhouse, washes away culture
Base, it is transplanted into hole tray, cover film, while film is removed after shade 75%, 2 weeks, give full exposure, carries out rich water quality management.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included in the interest field that the present invention is limited within protection scope of the present invention.
Claims (5)
- A kind of 1. iris root media, it is characterised in that including：MS+0.5mg/L~1mg/L 6-BA+ activated carbons+sucrose+35g/L+ agar 5g/L.
- 2. a kind of iris root media according to claim 1, it is characterised in that also include：3mg/L~5mg/L TDZ.
- 3. a kind of iris root media according to claim 1, it is characterised in that also include：1mg/L~5mg/L PPP333.
- 4. a kind of iris root media according to claim 1, it is characterised in that the activated carbon is 1-5g/L.
- 5. a kind of iris root media according to claim 1, it is characterised in that the sucrose is 20-35g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711103652.8A CN107637524A (en) | 2017-11-10 | 2017-11-10 | A kind of iris root media |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711103652.8A CN107637524A (en) | 2017-11-10 | 2017-11-10 | A kind of iris root media |
Publications (1)
| Publication Number | Publication Date |
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| CN107637524A true CN107637524A (en) | 2018-01-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711103652.8A Pending CN107637524A (en) | 2017-11-10 | 2017-11-10 | A kind of iris root media |
Country Status (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109089883A (en) * | 2018-08-31 | 2018-12-28 | 贵州民族大学人文科技学院 | The in-vitro conservation method that a kind of river east lamp stand is heralded spring |
| CN109089880A (en) * | 2018-07-18 | 2018-12-28 | 云南省农业科学院生物技术与种质资源研究所 | A method of regeneration plant is obtained using Oryza eichingeri leaf sheath for explant |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4684612A (en) * | 1984-07-27 | 1987-08-04 | Sungene Technologies Corporation | Process for regenerating soybeans |
| CN102845311A (en) * | 2012-10-11 | 2013-01-02 | 山东鑫秋种业科技有限公司 | Method for preparing phalaenopsis plant tissue medium |
| CN104429950A (en) * | 2014-11-14 | 2015-03-25 | 佛山市顺德区今日景艺生物科技有限公司 | Phalaenopsis blume culture medium and culture method |
| CN106106163A (en) * | 2016-07-12 | 2016-11-16 | 成都东山兰韵农业有限公司 | A kind of iris cultivates propagation method |
-
2017
- 2017-11-10 CN CN201711103652.8A patent/CN107637524A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4684612A (en) * | 1984-07-27 | 1987-08-04 | Sungene Technologies Corporation | Process for regenerating soybeans |
| CN102845311A (en) * | 2012-10-11 | 2013-01-02 | 山东鑫秋种业科技有限公司 | Method for preparing phalaenopsis plant tissue medium |
| CN104429950A (en) * | 2014-11-14 | 2015-03-25 | 佛山市顺德区今日景艺生物科技有限公司 | Phalaenopsis blume culture medium and culture method |
| CN106106163A (en) * | 2016-07-12 | 2016-11-16 | 成都东山兰韵农业有限公司 | A kind of iris cultivates propagation method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109089880A (en) * | 2018-07-18 | 2018-12-28 | 云南省农业科学院生物技术与种质资源研究所 | A method of regeneration plant is obtained using Oryza eichingeri leaf sheath for explant |
| CN109089883A (en) * | 2018-08-31 | 2018-12-28 | 贵州民族大学人文科技学院 | The in-vitro conservation method that a kind of river east lamp stand is heralded spring |
| CN109089883B (en) * | 2018-08-31 | 2021-07-27 | 贵州民族大学人文科技学院 | In-vitro preservation method of primula sikkmensis franch |
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Application publication date: 20180130 |