CN107794225B - 一种微藻培养方法 - Google Patents
一种微藻培养方法 Download PDFInfo
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- CN107794225B CN107794225B CN201610791128.3A CN201610791128A CN107794225B CN 107794225 B CN107794225 B CN 107794225B CN 201610791128 A CN201610791128 A CN 201610791128A CN 107794225 B CN107794225 B CN 107794225B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Description
组成成分 | 基础培养基(g/L) | 补料培养基(g/L) |
葡萄糖 | 5~20 | 750 |
尿素 | 0.25~1.0 | 37.5 |
KH<sub>2</sub>PO<sub>4</sub> | 0.3~1.2 | 30 |
MgSO<sub>4</sub>·7H<sub>2</sub>O | 0.3~1.2 | 30 |
柠檬酸三钠 | 0.05~0.2 | 5 |
CaCl<sub>2</sub>母液 | 1mL | 12.5mL |
FeSO<sub>4</sub>与EDTA母液 | 1mL | 12.5mL |
微量元素母液 | 1mL | 12.5mL |
消泡剂 | 0.08mL | 750 |
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CN201610791128.3A CN107794225B (zh) | 2016-08-31 | 2016-08-31 | 一种微藻培养方法 |
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CN107794225A CN107794225A (zh) | 2018-03-13 |
CN107794225B true CN107794225B (zh) | 2022-09-13 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110195019A (zh) * | 2019-05-10 | 2019-09-03 | 中国科学院水生生物研究所 | 一种高产蛋白质小球藻的培养方法 |
CN113046247A (zh) * | 2019-12-26 | 2021-06-29 | 云南爱尔发生物技术股份有限公司 | 采用连续补料方式的微藻培养方法 |
CN111394253A (zh) * | 2020-04-03 | 2020-07-10 | 湛江国联水产开发股份有限公司 | 一种微拟球藻高密度连续培养方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101186880A (zh) * | 2007-11-29 | 2008-05-28 | 上海交通大学 | 异养培养小球藻的补料优化方法 |
CN105378055A (zh) * | 2013-07-26 | 2016-03-02 | 罗盖特兄弟公司 | 用于通过顺序、自动提供葡萄糖分批补料发酵所供养的小球藻属物种的方法 |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101186880A (zh) * | 2007-11-29 | 2008-05-28 | 上海交通大学 | 异养培养小球藻的补料优化方法 |
CN105378055A (zh) * | 2013-07-26 | 2016-03-02 | 罗盖特兄弟公司 | 用于通过顺序、自动提供葡萄糖分批补料发酵所供养的小球藻属物种的方法 |
Non-Patent Citations (1)
Title |
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"异养-胁迫"分段培养对原始小球藻生物量和油脂含量影响研究;汪桂林等;《中国生物工程杂志》;20130331;第33卷(第03期);第1.2.2节、2.1.1节 * |
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