CN107778100A - A kind of biological organic fertilizer of rod containing cigarette and bacillus amyloliquefaciens and preparation method thereof - Google Patents
A kind of biological organic fertilizer of rod containing cigarette and bacillus amyloliquefaciens and preparation method thereof Download PDFInfo
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- CN107778100A CN107778100A CN201711076249.0A CN201711076249A CN107778100A CN 107778100 A CN107778100 A CN 107778100A CN 201711076249 A CN201711076249 A CN 201711076249A CN 107778100 A CN107778100 A CN 107778100A
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- organic fertilizer
- bacillus amyloliquefaciens
- biological organic
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to a kind of rod containing cigarette and the biological organic fertilizer of bacillus amyloliquefaciens, and it includes the component of following parts by weight：0.1 0.5 parts of decomposed 70 ~ 130 parts of material, 2.5 ~ 10 parts of cigarette rod, 5 ~ 10 parts of plant ash and bacillus amyloliquefaciens.There is provided a kind of preparation method of above-mentioned biological organic fertilizer simultaneously.The present invention is a kind of with diseases prevention, the biological organic fertilizer of anthelmintic action.
Description
Technical field
The present invention relates to a kind of biological organic fertilizer, more precisely, is related to a kind of rod containing cigarette and bacillus amyloliquefaciens
Biological organic fertilizer and preparation method thereof.
Background technology
With China's economy and the improvement of people's living standards, people are increasing to the demand of vegetables, facilities vegetable
As the major way of China's vegetable cultivation, controlled because facilities vegetable is warm and humid, photo-thermal is sufficient and the growth of wrong season, facilities vegetable
Plantation economic benefit is far longer than outdoor cropping vegetables, turns into the mainstay of current rural economy.However, due to planting facility
Investment is big, can not suitable crop rotation, cause greenhouse vegetable soil salination is serious, soil disease aggravates, root-knot nematode increase,
Organic matter is reduced, soil granular structure is destroyed, therefore have and improved the soil, recover soil micro-ecosystem balance, drive away nematode work
Sustainable development of the biological organic fertilizer to facilities vegetable has the function that important.
At present, with going deep into vegetable production in green house obstacle understanding, the research to biological organic fertilizer development gradually increases,
The bio-organic fertilizer product with good effects is obtained.In recent years, domestic scholars are carried out to biological organic fertilizer and its formula
Numerous studies, develop using the agricultural wastes such as plant ash, mushroom slag, coconut husk, feces of livestock and poultry composition organic fertilizer and suitable
Applied on area and vegetables.But these bio-organic fertilizer products are little to the prevention effect of nematode, and pass through chemistry
Measure carry out nematode preventing and treating again bring serious environmental pollution.Therefore, carry out with expelling parasite, diseases prevention, improved soil effect
Biological organic fertilizer research and development to ensure China's facilities vegetable sustainable development have great importance.
The content of the invention
It is an object of the invention to provide a kind of biological organic fertilizer with diseases prevention, anthelmintic action, while provide in one kind
State the preparation method of biological organic fertilizer.
The present invention adopts the following technical scheme that：
A kind of biological organic fertilizer of rod containing cigarette and bacillus amyloliquefaciens, it includes the component of following parts by weight：Decomposed material 70 ~
130 parts, 2.5 ~ 10 parts of cigarette rod, 5 ~ 10 parts of plant ash and bacillus amyloliquefaciens 0.1-0.5 parts.
Further, the decomposed material obtains through microorganism-decomposing agent to raw material progress high-temperature aerobic fermentation to completely decomposed
Arrive；The raw material includes the component of following parts by weight：15 ~ 30 parts of chicken manure, 10 ~ 30 parts of cow dung, 10 ~ 20 parts of sheep manure, mushroom residue 15 ~
30 parts and 10 ~ 20 parts of stalk；The addition of the microorganism-decomposing agent is the 0.1-0.5% of material quality.
Further, biological organic fertilizer includes the component of following parts by weight：Decomposed 90 parts of material, 6 parts of cigarette rod, 8 parts of plant ash
With 0.25 part of bacillus amyloliquefaciens.
Further, the decomposed material obtains through microorganism-decomposing agent to raw material progress high-temperature aerobic fermentation to completely decomposed
Arrive；The raw material includes the component of following parts by weight：15 parts of chicken manure, 20 parts of cow dung, 15 parts of sheep manure, 25 parts of mushroom residue and stalk 15
Part；The addition of the microorganism-decomposing agent is the 0.3% of material quality.
Further, the leftover bits and pieces that the cigarette rod produces for tobacco factory's cigarette, including offal, offal etc..
Further, the bacillus amyloliquefaciens be bacillus amyloliquefaciens after liquid fermentation, using calcium carbonate as
The spray-dried gained of adsorbent.The specific method being spray-dried after calcium carbonate absorption bacillus amyloliquefaciens is known skill
Art, the dosage of calcium carbonate are suitable effectively to adsorb bacillus amyloliquefaciens bacterium solution.
Further, the bacillus amyloliquefaciens are the solution starch gemma bar that deposit number is CGMCC No. 12190
Bacterium（Bacillus amyloliquefaciens）.
Further, the viable count of the bacillus amyloliquefaciens is 10 × 108cfu/g~100×108cfu/g。
Further, the viable count of the bacillus amyloliquefaciens is 10 × 108cfu/g~50×108cfu/g。
A kind of preparation method of above-mentioned biological organic fertilizer, it comprises the following steps：
（a）By bacillus amyloliquefaciens Liquid Culture to gemma is produced, spray drying is carried out as adsorbent using calcium carbonate and solved
Bacillus amyloliquefacienses bacterium powder, the bacterium number in the bacterium powder are 10 × 108cfu/g~100×108cfu/g；
（b）Chicken manure, cow dung, sheep manure, mushroom residue and stalk are mixed by the parts by weight, add the 0.1-0.5% of mixture quality
Microorganism-decomposing agent carry out high-temperature aerobic fermentation to completely it is decomposed, seed germination index is more than 85%；
（c）Cigarette rod, plant ash and the bacillus amyloliquefaciens bacterium powder added in decomposed material after crushing.
The beneficial effects of the present invention are：
The cigarette rod that the present invention is directed to caused offal in cigarette production process, the compression of offal the like waste forms carries out its application
The exploitation in footpath, and it is current, and above-mentioned offal and offal there is no very effective purposes.The present invention is exactly using special caused by tobacco
Different smell, the biological organic fertilizer with unique effects with other solid waste and antagonistic microbe formation.
The present invention, to deinsectization and the shortcoming of diseases prevention effect, is carried out containing cigarette rod and the micro- life of function for current biological organic fertilizer
The exploitation of the biological organic fertilizer of thing, make biological organic fertilizer that there is the multi-efficiency of preventing and treating nematode, soil-borne disease and improved soil.
Embodiment
Technical scheme is set forth in below in conjunction with preferred embodiment.Following examples are used only for
The description and interpretation present invention, without forming the limitation to technical solution of the present invention.
The influence that the cigarette rod of embodiment 1 grows to function stem
Cigarette rod is dipped to clear water soft, is added to by 1%, 3%, 5%, 7%, 9% amount in LB culture mediums, plate is down flat after sterilizing,
It is 5 × 10 by concentration8Cfu/g bacillus amyloliquefaciens（Deposit number is CGMCC No. 12190）, it is bacillus subtilis, huge
Bacterium anthracoides and rhizobium bacterium powder are diluted and apply flat board step by step, to be not added with the LB culture mediums of cigarette rod as control, as a result
Show, wherein its corresponding control bacterium number of the bacterium number on the cigarette rod culture medium of each concentration of culture bacillus amyloliquefaciens is compared
It is slightly higher, therebetween in the absence of significant difference.And the Time transfer receiver that bacillus amyloliquefaciens bacterium colony occurs on cigarette rod culture medium
According to early, colony diameter illustrates that cigarette rod can promote bacillus amyloliquefaciens to grow also greater than control flat board on flat board.Analyze withered grass
The bacterium number and growth conditions of bacillus, bacillus megaterium, rhizobium on cigarette rod LB culture mediums and LB culture mediums, the suppression of cigarette rod
Make the growth of these three bacterium.Show, bacillus amyloliquefaciens can be compounded with cigarette rod.
Embodiment 2
A kind of facilities vegetable biological organic fertilizer, the matrix include cigarette rod, chicken manure, cow dung, cow dung, sheep manure, plant ash, mushroom
Slag, stalk and bacillus amyloliquefaciens.
The weight ratio of the biological organic fertilizer composition of raw materials is：10 parts of cigarette rod, 23 parts of chicken manure, 30 parts of cow dung, 10 parts of sheep manure,
30 parts of mushroom residue, 10 parts of stalk.
Add 0.1% decomposed microbial bacterial agent and above-mentioned raw material carry out high-temperature aerobic fermentation to completely decomposed, measure kind
Sub- germination index is 88%.
Bacillus amyloliquefaciens are subjected to conventional liq fermentation, acquisition concentration be spray-dried as 100 after obtaining gemma
×108Cfu/g bacterium powder
10 parts of cigarette rod, 10 parts of plant ash, 0.1 part of bacillus amyloliquefaciens bacterium powder are added into decomposed material.
The biological organic fertilizer water content 22%, it is 55% that organic matter, which contains, functional microorganism content >=0.2 hundred million/g.
Embodiment 3
A kind of facilities vegetable biological organic fertilizer, the matrix include cigarette rod, chicken manure, cow dung, cow dung, sheep manure, plant ash, mushroom
Slag, stalk and bacillus amyloliquefaciens.
The weight ratio of the biological organic fertilizer composition of raw materials is：30 parts of chicken manure, 10 parts of cow dung, 20 parts of sheep manure, 15 parts of mushroom residue,
20 parts of stalk.
Add 0.3% decomposed microbial bacterial agent and above-mentioned raw material carry out high-temperature aerobic fermentation to completely decomposed, measure kind
Sub- germination index is 90%.
Bacillus amyloliquefaciens are subjected to conventional liq fermentation, after obtaining gemma, calcium carbonate is that carrier is spray-dried,
It is 100 × 10 to obtain concentration8Cfu/g bacterium powder.
2.5 parts of cigarette rod, 6 parts of plant ash, 0.5 part of bacillus amyloliquefaciens bacterium powder are added into decomposed material.
The biological organic fertilizer water content 20%, it is 48% that organic matter, which contains, functional microorganism content >=0.2 hundred million/g.
Embodiment 4
A kind of facilities vegetable biological organic fertilizer, the matrix include cigarette rod, chicken manure, cow dung, cow dung, sheep manure, plant ash, mushroom
Slag, stalk and bacillus amyloliquefaciens.
The parts by weight of the biological organic fertilizer composition of raw materials are：15 parts of chicken manure, 20 parts of cow dung, 15 parts of sheep manure, mushroom residue 25
Part, 15 parts of stalk.
Add 0.5% decomposed microbial bacterial agent and above-mentioned raw material carry out high-temperature aerobic fermentation to completely decomposed, measure kind
Sub- germination index is 95%.
Bacillus amyloliquefaciens are subjected to conventional liq fermentation, after obtaining gemma, calcium carbonate is that carrier is spray-dried,
It is 100 × 10 to obtain concentration8Cfu/g bacterium powder.
6 parts of cigarette rod, 8 parts of plant ash, 0.25 part of bacillus amyloliquefaciens bacterium powder are added into decomposed material.
The biological organic fertilizer water content 16%, it is 52% that organic matter, which contains, functional microorganism content >=0.2 hundred million/g.
The contrast test of fertilizer efficiency of different biological organic fertilizers
Biological organic fertilizer prepared by embodiment 2 ~ 4 can be applied in the various vegetables kind such as cucumber, tomato, eggplant, and this is sent out
1 kind of biological organic fertilizer CK1 of facilities vegetable biological organic fertilizer and the in the market purchase of bright preparation, separately prepare a kind of life of smokeless rod
Organic fertilizer is used for cucumber base fertilizer, and contrast test effect is as follows：
The fertilizer application phase is on January 8th, 2016, and broadcast application 300 kgs/acre, carries out soil rotation in soil surface before site preparation
Cultivated and site preparation.By the uniform transplantation of seedlings of growing way, to 5 years, continuous plantation, the preceding first quarter occurred in the greenhouse of root-knot nematode, and statistics is yellow
A situation arises for melon product, root-knot nematode.0 grade：Without root knot；1 grade：Old complaint knot is seldom, has root knot radical to account for the 5% of whole root system； 2
Level：Old complaint root knot is less, there is the 6%-20% that root knot radical accounts for whole root system；3 grades：There is more root knot in old complaint, there is root knot radical
Account for the 21%-40% of whole root system；4 grades：There is more root knot in old complaint, there is the 41%-70% that root knot radical accounts for whole root system；5 grades：Disease
Root root knot interlinks into fibrous root group, has root knot radical to account for more than the 71% of whole root system.
Cucumber root sense insect pest situation condition, with root knot exponential representation, calculation formula is：
Root knot index（%）=∑（Each sick level plant number × level value）/（Investigate total strain number × most grave illness level value）×100.
Statistical result showed, having described in the facilities vegetable biological organic fertilizer described in embodiment 2 ~ 4, particularly embodiment 4
Machine fertilizer, its leaf color is glossy dark green, plant droop, root-knot nematode incidence reduce, yield higher than other processing, with embodiment 2 ~
What 4 fertilizer were compared lacks the biological organic fertilizer of cigarette rod, and its root-knot nematode incidence illustrates cigarette rod composition apparently higher than embodiment
Prevent and treat the effect of root-knot nematode.
In addition, the biological organic fertilizer of non-smoke adding rod to the preventive effect of cucumber fusarium axysporum also below the fertilizer of embodiment 1 ~ 4, indicate
Growth promoting and synergistic effect of the cigarette rod to bacillus amyloliquefaciens.
In a word, the fertilizer of embodiment 2 ~ 4 has good diseases prevention, deinsectization, growth promoting effect.
The fertilizer efficiency and drug effect of 1 different biological organic fertilizers of table processing
。
Embodiment described above is only that the preferred embodiment of the present invention is described, but is not limited to this, this
The technical staff in field is easy to understand according to above-described embodiment the spirit of the present invention, and makes different amplification and change, but
Without departing from the spirit of the present invention, all within protection scope of the present invention.
Claims (9)
1. the biological organic fertilizer of a kind of rod containing cigarette and bacillus amyloliquefaciens, it is characterised in that it includes the group of following parts by weight
Point：Decomposed 70 ~ 130 parts of material, 2.5 ~ 10 parts of cigarette rod, 5 ~ 10 parts of plant ash and bacillus amyloliquefaciens 0.1-0.5 parts.
2. biological organic fertilizer according to claim 1, it is characterised in that the decomposed material is through microorganism-decomposing agent to raw material
High-temperature aerobic fermentation is carried out to obtain to completely decomposed；The raw material includes the component of following parts by weight：15 ~ 30 parts of chicken manure, cow dung
10 ~ 20 parts of 10 ~ 30 parts, 10 ~ 20 parts of sheep manure, 15 ~ 30 parts of mushroom residue and stalk；The addition of the microorganism-decomposing agent is raw material
The 0.1-0.5% of quality.
3. biological organic fertilizer according to claim 1, it is characterised in that it includes the component of following parts by weight：Decomposed material
0.25 part of 90 parts, 6 parts of cigarette rod, 8 parts of plant ash and bacillus amyloliquefaciens.
4. biological organic fertilizer according to claim 3, it is characterised in that the decomposed material is through microorganism-decomposing agent to raw material
High-temperature aerobic fermentation is carried out to obtain to completely decomposed；The raw material includes the component of following parts by weight：15 parts of chicken manure, cow dung 20
Part, 15 parts of sheep manure, 25 parts of mushroom residue and 15 parts of stalk；The addition of the microorganism-decomposing agent is the 0.3% of material quality.
5. according to the biological organic fertilizer described in any one of claim 1 ~ 4, it is characterised in that the bacillus amyloliquefaciens are solution
Bacillus amyloliquefacienses are used as the spray-dried gained of adsorbent after liquid fermentation, using calcium carbonate.
6. biological organic fertilizer according to claim 5, it is characterised in that the bacillus amyloliquefaciens are that deposit number is
CGMCC No. 12190 bacillus amyloliquefaciens.
7. biological organic fertilizer according to claim 6, it is characterised in that the viable count of the bacillus amyloliquefaciens is 10
×108cfu/g~100×108cfu/g。
8. biological organic fertilizer according to claim 7, it is characterised in that the viable count of the bacillus amyloliquefaciens is 10
×108cfu/g~50×108cfu/g。
It is 9. a kind of such as the preparation method of any one of claim 1 ~ 8 biological organic fertilizer, it is characterised in that it includes following step
Suddenly：
（a）By bacillus amyloliquefaciens Liquid Culture to gemma is produced, spray drying is carried out as adsorbent using calcium carbonate and solved
Bacillus amyloliquefacienses bacterium powder, the bacterium number in the bacterium powder are 10 × 108cfu/g~100×108cfu/g；
（b）Chicken manure, cow dung, sheep manure, mushroom residue and stalk are mixed by the parts by weight, add the 0.1-0.5% of mixture quality
Microorganism-decomposing agent carry out high-temperature aerobic fermentation to completely it is decomposed, seed germination index is more than 85%；
（c）Cigarette rod, plant ash and the bacillus amyloliquefaciens bacterium powder added in decomposed material after crushing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711076249.0A CN107778100A (en) | 2017-11-06 | 2017-11-06 | A kind of biological organic fertilizer of rod containing cigarette and bacillus amyloliquefaciens and preparation method thereof |
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| CN201711076249.0A CN107778100A (en) | 2017-11-06 | 2017-11-06 | A kind of biological organic fertilizer of rod containing cigarette and bacillus amyloliquefaciens and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108409507A (en) * | 2018-06-13 | 2018-08-17 | 东营市昶盛农业科技有限公司 | A kind of preparation method of the applicable Lenlinus edodes residue organic fertilizer of round eggplant |
| CN114478136A (en) * | 2022-02-11 | 2022-05-13 | 河北馨仙养殖有限公司 | Disease and pest prevention full-nutrition bio-organic fertilizer and preparation method and application thereof |
| CN114890848A (en) * | 2022-06-09 | 2022-08-12 | 河北省畜牧兽医研究所 | Disease and pest prevention full-nutrition bio-organic fertilizer, preparation method and application |
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| CN114890848A (en) * | 2022-06-09 | 2022-08-12 | 河北省畜牧兽医研究所 | Disease and pest prevention full-nutrition bio-organic fertilizer, preparation method and application |
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