CN106883011A - A kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus and preparation method thereof - Google Patents
A kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus and preparation method thereof Download PDFInfo
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The present invention provides a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus and preparation method thereof, and the preparation method of the biological organic fertilizer is：Biomass material is ground into powder, is placed in resistant to elevated temperatures closed container, carbonization treatment is carried out under 300 700 DEG C, anoxia condition, crushed after being cooled to room temperature, obtain biomass charcoal powder；The rotten fermentation of agricultural crop straw powder heap, sieving are formed into the rotten material of agricultural crop straw；Methylotrophic bacillus is formed into Methylotrophic fermentation of bacillus liquid through inoculation, culture, fermentation；Biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and turf are poured into mixer and is adsorbed, after well mixed, the rotten material of agricultural crop straw, mineral matter and desulfurated plaster are progressively added toward mixer, material is crushed after stirring, sieving, adjustment material moisture and pH, obtain final product the bio-organic fertilizer based on charcoal and Methylotrophic bacillus.
Description
Technical field
The invention belongs to technical field of biological organic fertilizer, and in particular to one kind is based on charcoal and Methylotrophic gemma bar
Biological organic fertilizer of bacterium and preparation method thereof.
Background technology
Charcoal is the biomass organic substance rich in carbon that slow pyrolytic is obtained under the conditions of oxygen-free environment, biological
Charcoal in soil can stable for extended periods of time, can improve soil physico-chemical property and improve edaphon activity, increase soil
The earth content of organic matter, and the hole of charcoal is micro/nano level, can store moisture and nutrient, can improve Soil Microorganism
Quantity and activity, therefore charcoal can be as soil conditioner, fertilizer slow release carrier and carbon source.
Methylotrophic bacillus is one plant of marine bacteria, the material pair produced after the culture of Methylotrophic bacillus
Plant epiphyte class disease has stronger antibacterial activity, and does not have harmful effect, no pathogenicity, environmental sound to people and animals.
One plant of Methylotrophic bacillus ZY3A disclosed in Chinese patent CN 105925504A and its promote Lettuce growth on
Using, a small amount of Methylotrophic bacillus ZY3A strains are lined into LB flat boards, incubated overnight obtains pre-culture bacterium colony,
Pre-culture bacterium colony is placed in beef-protein medium, after 30 DEG C of culture 12h in fermentation tank, nutrient solution is obtained, will
The growth and disease and insect resistance that Lettuce can be promoted after nutrient solution dilution are acted on.It is a kind of disclosed in Chinese patent CN 105384572A
Straw biological fertilizer and its preparation method and application, straw biological is made with straw and bacillus subtilis as feasible material
Fertilizer, bacillus subtilis makes straw be fermented in soil, through granulating, screening and being dried to obtain straw biological fertilizer.By above-mentioned
Prior art understands that bacillus compound with the other materials such as stalk can prepare bio-fertilizer, to heighten the comprehensive of bio-fertilizer
Can, but the report being currently based in terms of the biological organic fertilizer of charcoal and Methylotrophic bacillus is actually rare.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of biology based on charcoal and Methylotrophic bacillus
Organic fertilizer and preparation method thereof, using stalk as primary raw material, obtains biomass carbon and stalk through carbonization and corruption treatment respectively
Auxiliary material, then mix with Methylotrophic fermentation of bacillus liquid, turf, desulfurated plaster and mineral matter auxiliary obtain biological organic
Fertilizer, the biological organic fertilizer makes full use of stalk to recycle resource, and wide material sources are with low cost, and can improve soil environment,
Effectively prevention soil-borne disease, improves the quality and yield of crop.
In order to solve the above technical problems, the technical scheme is that：
A kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus, the biological organic fertilizer includes biology
The rotten material of matter powdered carbon, microbial function bacterium, turf, agricultural crop straw, mineral matter and desulfurated plaster, the raw material of the biomass charcoal powder
It is one or more in wheat stalk, maize straw, rice straw, cotton stalk, peanut shell, fruit tree pruning, shrub branch
Mixture, the microbial function bacterium is Methylotrophic fermentation of bacillus liquid, the raw material of the rotten material of the agricultural crop straw
It is the mixing of one or more in cassava stalk, sugarcane cauline leaf, mango branches and leaves, longan branches and leaves, maize straw, pineapple stem and leaf
Thing, the mineral matter is the mixture of one or more in zeolite powder, vermiculite power, diatomite, white carbon, precipitated calcium carbonate.
As the preferred of above-mentioned technical proposal, the biological organic fertilizer based on charcoal and Methylotrophic bacillus
In component, by weight percentage, including biomass charcoal powder 3-5%, microbial function bacterium 5-10%, turf 5-10%, ore deposit
Material 13-20%, desulfurated plaster 10-15%, remaining is the rotten material of agricultural crop straw, and the total amount of above mass percentage content is
100%.
The present invention also provides a kind of preparation method of the biological organic fertilizer based on charcoal and Methylotrophic bacillus,
Comprise the following steps：
(1) biomass material is ground into powder, is placed in resistant to elevated temperatures closed container, in 300-700 DEG C, anoxia condition
Under carry out carbonization treatment 1-3h, be cooled to after room temperature and crush, obtain biomass charcoal powder；
(2) by the rotten fermentation of agricultural crop straw powder heap, 60-80 mesh sieves are crossed, forms the rotten material of agricultural crop straw；
(3) Methylotrophic bacillus is formed into Methylotrophic fermentation of bacillus liquid through inoculation, culture, fermentation；
(4) biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and the turf for preparing step (1) pour into mixer
In adsorbed, after being well mixed, progressively added toward mixer step (2) preparation agricultural crop straw it is rotten expect, mineral matter and
Desulfurated plaster, is crushed material after stirring, and crosses 100 mesh sieves, adjusts material moisture and pH, is obtained final product based on charcoal
With the bio-organic fertilizer of Methylotrophic bacillus.
Used as the preferred of above-mentioned technical proposal, in the step (1), the particle diameter of biomass charcoal powder is less than 1mm.
Used as the preferred of above-mentioned technical proposal, in the step (2), the condition of the rotten fermentation of heap is：Water content is 55-
60%, temperature is 40-65 DEG C, and the time is 7-15d.
As the preferred of above-mentioned technical proposal, in the step (3), the specific system of Methylotrophic fermentation of bacillus liquid
Preparation Method is：The Methylotrophic Bacillus strain that will be stored under -4 DEG C of environment is seeded on beef peptone flat board, and 30
DEG C culture 48h, jump go single bacterium colony to be seeded in beef extract-peptone fluid nutrient medium, 30 DEG C culture 24h after obtain inoculation liquid, will
Inoculation liquid is inoculated in the 300L seeding tanks equipped with seed culture medium, and it is 10% to connect bacterium amount, is fermented after connecing bacterium, initial pH value
6.9-7.1,28-35 DEG C of temperature, rotating speed 180-220r/min, as seeding tank fermentation 9-10h, thalli growth is in logarithmic phase, this
When the zymotic fluid in seeding tank be added to 3000L fermentation tanks equipped with fermentation medium relay supervention ferment, control condition is of the same race
Sub- tank, cultivates 28-30h, as thalline sporulation 90-95%, terminates fermentation, obtains Methylotrophic gemma zymotic fluid.
Used as the preferred of above-mentioned technical proposal, the parameter of the beef extract-peptone fluid nutrient medium is：Beef extract 5g, egg
White peptone 10g, agar 15-20g, pH 7.2-7.6, the parameter of the seed culture medium is：Glucose 10g/L, peptone 2g/L,
MgSO4 0.5g/L, yeast extract 2g/L, beef extract 2g/L, the parameter of the fermentation medium is：Corn flour 30g/L, beancake powder
20g/L, MgSO40.5g/L, NaH2PO40.5g/L, CaCl22H2O 1g/L, K2HPO4 0.5g/L。
As the preferred of above-mentioned technical proposal, the beef extract-peptone fluid nutrient medium, seed culture medium and fermentation training
The sterilising conditions for supporting base are 0.1-0.15MPa, 121 DEG C of sterilizing 30min.
As the preferred of above-mentioned technical proposal, in the step (3), the moisture of Methylotrophic gemma bacillus agent
≤ 29%, pH value is 6.9-7.5.
As the preferred of above-mentioned technical proposal, in the step (4), based on charcoal and Methylotrophic bacillus
Component in bio-organic fertilizer, by weight percentage, including biomass charcoal powder 3-5%, microbial function bacterium 5-10%, grass
Charcoal 5-10%, mineral matter 13-20%, desulfurated plaster 10-15%, remaining is the rotten material of agricultural crop straw, and above mass percent contains
The total amount of amount is 100%.
Compared with prior art, the invention has the advantages that：
(1) bio-organic fertilizer based on charcoal and Methylotrophic bacillus of preparation of the invention contains biology
Powdered carbon, the rotten material of turf and agricultural crop straw, the abundant organic matter contained in stalk, nitrogen, phosphorus, potassium, calcium, magnesium, silicon, sulphur and other
Trace element, as the organic fertilizer resource of crop, turf is replaced in the present invention using biological powdered carbon and the rotten material part of agricultural crop straw,
Both the utilization rate of turf can have been reduced, energy stalk resource can have again been recycled again, improve the economic worth of stalk resource,
The problems such as environmental pollution brought by crop straw burning can also be alleviated.
(2) bio-organic fertilizer based on charcoal and Methylotrophic bacillus of preparation of the invention contains methyl
Auxotype bacillus, the bacterium can adjust crop microbial ecological area to person poultry harmless, improve crop quality, and withered to plant
Disease, the root rot of withering all show stronger prevention effect.
(3) containing de- in the bio-organic fertilizer based on charcoal and Methylotrophic bacillus of preparation of the invention
Sulphur gypsum has the features such as renewable, granularity is small, stable components, and objectionable impurities content is few, purity is high, and desulfurated plaster can be fast
The saline alkali of velocity modulation section soil, promotes the acid-base balance of soil, then improves the yield and quality of crop.
(4) bio-organic fertilizer based on charcoal and Methylotrophic bacillus of preparation of the invention also has biology
Charcoal and biological organic fertilizer, being combined by both can both adjust the physical property of soil, reduce the unit weight and the degree of packing of soil,
Increase the water retention property of soil, the biomass of increase microorganism, improving the mineralization degree of enzymatic activity and nitrogen, moreover it is possible to promote root of the crop
The growth of system, in addition containing containing health, without fat, mineral matter can not only maintain the existence of Methylotrophic bacillus in product, also
The content of trace element in product can be increased.
(5) the biological organic fertilizer preparation method for material based on charcoal and Methylotrophic bacillus of preparation of the invention
Simply, the biological organic fertilizer instant effect of preparation, low cost, can be used for the cultivation of various soil and crop.
Specific embodiment
Describe the present invention in detail below in conjunction with specific embodiment, herein illustrative examples of the invention and explanation
It is for explaining the present invention but not as a limitation of the invention.
Embodiment 1：
(1) biomass material wheat stalk is broken into the powder of 0.5mm, is placed in resistant to elevated temperatures closed container, 300
DEG C, carry out carbonization treatment 2h under anoxia condition, crushed after being cooled to room temperature, obtain diameter less than 0.25mm, proportion is 0.5-
0.8g/cm3Biomass charcoal powder.
(2) by agricultural crop straw sugarcane cauline leaf and the rotten fermentation of pineapple stem and leaf heap, water content is 55%, and temperature is 40 DEG C, when
Between be 7d, cross 80 mesh sieves, form the rotten material of agricultural crop straw.
(3) the Methylotrophic Bacillus strain that will be stored under -4 DEG C of environment is seeded on beef peptone flat board,
30 DEG C of culture 48h, jump goes single bacterium colony to be seeded in beef extract-peptone fluid nutrient medium, and inoculation liquid is obtained after 30 DEG C of culture 24h,
Inoculation liquid is inoculated in the 300L seeding tanks equipped with seed culture medium, it is 10% to connect bacterium amount, is fermented after connecing bacterium, initial pH
Value 6.9,28 DEG C of temperature, rotating speed 180r/min, as seeding tank fermentation 9h, thalli growth is in logarithmic phase, now by seeding tank
Zymotic fluid be added to equipped with fermentation medium 3000L fermentation tanks relaying supervention ferment, the same seeding tank of control condition, cultivate 28h,
When thalline sporulation 90%, terminate fermentation, it is 25.8% to obtain moisture, and pH value is 7.4 Methylotrophic gemma
Zymotic fluid, the parameter of wherein beef extract-peptone fluid nutrient medium is：Beef extract 5g, peptone 10g, agar 15g, pH 7.2,
The parameter of the seed culture medium is：Glucose 10g/L, peptone 2g/L, MgSO4 0.5g/L, yeast extract 2g/L, beef extract
2g/L, the parameter of fermentation medium is：Corn flour 30g/L, beancake powder 20g/L, MgSO40.5g/L, NaH2PO40.5g/L,
CaCl22H2O 1g/L, K2HPO40.5g/L, beef extract-peptone fluid nutrient medium, seed culture medium and fermentation medium
Sterilising conditions are 0.1MPa, 121 DEG C of sterilizing 30min.
(4) biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and turf are poured into mixer and is adsorbed, mixed
After closing uniformly, the rotten material of agricultural crop straw, zeolite powder mineral matter and desulfurated plaster are progressively added toward mixer, will after stirring
Material is crushed, and crosses 100 mesh sieves, adjusts material moisture and pH, is obtained final product based on charcoal and Methylotrophic bacillus
Bio-organic fertilizer, wherein the component in the bio-organic fertilizer based on charcoal and Methylotrophic bacillus, by weight
Amount percentages, including biomass charcoal powder 5%, microbial function bacterium 5%, turf 5%, mineral matter 13%, desulfurated plaster 15%,
Remaining is the rotten material of agricultural crop straw, and the total amount of above mass percentage content is 100%.
Embodiment 2：
(1) biomass material fruit tree pruning is ground into the powder of 0.5mm sizes, is placed in resistant to elevated temperatures closed container,
Carbonization treatment 3h is carried out under 300 DEG C, anoxia condition, is crushed after being cooled to room temperature, obtain diameter less than 0.25mm, proportion is
1.0g/cm3Biomass charcoal powder.
(2) by agricultural crop straw sugarcane cauline leaf and the rotten fermentation of pineapple stem and leaf heap, water content is 60%, and temperature is 65 DEG C, when
Between be 15d, cross 70 mesh sieves, form the rotten material of agricultural crop straw.
(3) the Methylotrophic Bacillus strain that will be stored under -4 DEG C of environment is seeded on beef peptone flat board,
30 DEG C of culture 48h, jump goes single bacterium colony to be seeded in beef extract-peptone fluid nutrient medium, and inoculation liquid is obtained after 30 DEG C of culture 24h,
Inoculation liquid is inoculated in the 300L seeding tanks equipped with seed culture medium, it is 10% to connect bacterium amount, is fermented after connecing bacterium, initial pH
Value 7.1,35 DEG C of temperature, rotating speed 220r/min, as seeding tank fermentation 10h, thalli growth is in logarithmic phase, now by seeding tank
Zymotic fluid be added to equipped with fermentation medium 3000L fermentation tanks relaying supervention ferment, the same seeding tank of control condition, cultivate 28-
30h, when thalline sporulation 95%, terminates fermentation, and it is 28.2% to obtain moisture, and pH value is 6.9 Methylotrophic
Gemma zymotic fluid, the parameter of wherein beef extract-peptone fluid nutrient medium is：Beef extract 5g, peptone 10g, agar 20g, pH
7.6, the parameter of the seed culture medium is：Glucose 10g/L, peptone 2g/L, MgSO4 0.5g/L, yeast extract 2g/L, ox
Meat extract 2g/L, the parameter of fermentation medium is：Corn flour 30g/L, beancake powder 20g/L, MgSO40.5g/L, NaH2PO4 0.5g/
L, CaCl22H2O 1g/L, K2HPO40.5g/L, beef extract-peptone fluid nutrient medium, seed culture medium and fermentation medium
Sterilising conditions be 0.15MPa, 121 DEG C sterilizing 30min.
(4) biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and turf are poured into mixer and is adsorbed, mixed
After closing uniformly, agricultural crop straw corruption material, zeolite powder, vermiculite power, diatomite, white carbon, lightweight carbon are progressively added toward mixer
Sour calcium mineral matter and desulfurated plaster, are crushed material after stirring, and cross 100 mesh sieves, adjust material moisture and pH, i.e.,
The bio-organic fertilizer of charcoal and Methylotrophic bacillus must be based on, wherein based on charcoal and Methylotrophic gemma
Component in the bio-organic fertilizer of bacillus, by weight percentage, including biomass charcoal powder 3%, microbial function bacterium 10%,
Turf 10%, mineral matter 20%, desulfurated plaster 15%, remaining is the rotten material of agricultural crop straw, above mass percentage content it is total
Measure is 100%.
Embodiment 3：
(1) biomass material corn stalk powder is broken into the powder of 1mm, is placed in resistant to elevated temperatures closed container, 700
DEG C, carry out carbonization treatment 1h under anoxia condition, crushed after being cooled to room temperature, obtain diameter less than 0.5mm, proportion is 1.2g/cm3
Biomass charcoal powder.
(2) by agricultural crop straw sugarcane cauline leaf and the rotten fermentation of pineapple stem and leaf heap, water content is 58%, and temperature is 50 DEG C, when
Between be 10d, cross 60 mesh sieves, form the rotten material of agricultural crop straw.
(3) the Methylotrophic Bacillus strain that will be stored under -4 DEG C of environment is seeded on beef peptone flat board,
30 DEG C of culture 48h, jump goes single bacterium colony to be seeded in beef extract-peptone fluid nutrient medium, and inoculation liquid is obtained after 30 DEG C of culture 24h,
Inoculation liquid is inoculated in the 300L seeding tanks equipped with seed culture medium, it is 10% to connect bacterium amount, is fermented after connecing bacterium, initial pH
Value 7.0,30 DEG C of temperature, rotating speed 200r/min, as seeding tank fermentation 9.5h, thalli growth is in logarithmic phase, now by seeding tank
In zymotic fluid be added to equipped with fermentation medium 3000L fermentation tanks relaying supervention ferment, the same seeding tank of control condition, culture
29h, when thalline sporulation 92%, terminates fermentation, and it is 26.3% to obtain moisture, and pH value is 7.2 Methylotrophic
Gemma zymotic fluid, the parameter of wherein beef extract-peptone fluid nutrient medium is：Beef extract 5g, peptone 10g, agar 18g, pH
7.5, the parameter of the seed culture medium is：Glucose 10g/L, peptone 2g/L, MgSO40.5g/L, yeast extract 2g/L, ox
Meat extract 2g/L, the parameter of fermentation medium is：Corn flour 30g/L, beancake powder 20g/L, MgSO40.5g/L, NaH2PO4 0.5g/
L, CaCl22H2O 1g/L, K2HPO40.5g/L, beef extract-peptone fluid nutrient medium, seed culture medium and fermentation medium
Sterilising conditions be 0.12MPa, 121 DEG C sterilizing 30min.
(4) biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and turf are poured into mixer and is adsorbed, mixed
After closing uniformly, the rotten material of agricultural crop straw, vermiculite power and precipitated calcium carbonate mineral matter and desulfurated plaster are progressively added toward mixer,
Material is crushed after stirring, is crossed 100 mesh sieves, adjusted material moisture and pH, obtained final product and sought based on charcoal and methyl
The bio-organic fertilizer of the type of supporting bacillus, wherein in the bio-organic fertilizer based on charcoal and Methylotrophic bacillus
Component, by weight percentage, including biomass charcoal powder 5%, microbial function bacterium 8%, turf 6%, mineral matter 15%, de-
Sulphur gypsum 10%, remaining is the rotten material of agricultural crop straw, and the total amount of above mass percentage content is 100%.
Embodiment 4：
(1) biomass material rice straw and cotton stalk are ground into the powder of 0.1mm, resistant to elevated temperatures closed appearance is placed in
In device, carbonization treatment 1-3h is carried out under 300-700 DEG C, anoxia condition, crushed after being cooled to room temperature, obtained diameter and be less than
0.5mm, proportion is 0.9g/cm3Biomass charcoal powder.
(2) by agricultural crop straw cassava stalk and the rotten fermentation of mango branches and leaves heap, water content is 56%, and temperature is 55 DEG C, when
Between be 12d, cross 70 mesh sieves, form the rotten material of agricultural crop straw.
(3) the Methylotrophic Bacillus strain that will be stored under -4 DEG C of environment is seeded on beef peptone flat board,
30 DEG C of culture 48h, jump goes single bacterium colony to be seeded in beef extract-peptone fluid nutrient medium, and inoculation liquid is obtained after 30 DEG C of culture 24h,
Inoculation liquid is inoculated in the 300L seeding tanks equipped with seed culture medium, it is 10% to connect bacterium amount, is fermented after connecing bacterium, initial pH
Value 6.9,35 DEG C of temperature, rotating speed 180r/min, as seeding tank fermentation 10h, thalli growth is in logarithmic phase, now by seeding tank
Zymotic fluid be added to equipped with fermentation medium 3000L fermentation tanks relaying supervention ferment, the same seeding tank of control condition, cultivate 28h,
When thalline sporulation 93%, terminate fermentation, obtain moisture≤29%, pH value is 7.2 Methylotrophic gemma hair
Zymotic fluid, the parameter of wherein beef extract-peptone fluid nutrient medium is：Beef extract 5g, peptone 10g, agar 15-20g, pH 7.5,
The parameter of the seed culture medium is：Glucose 10g/L, peptone 2g/L, MgSO4 0.5g/L, yeast extract 2g/L, beef extract
2g/L, the parameter of fermentation medium is：Corn flour 30g/L, beancake powder 20g/L, MgSO40.5g/L, NaH2PO40.5g/L,
CaCl22H2O 1g/L, K2HPO40.5g/L, beef extract-peptone fluid nutrient medium, seed culture medium and fermentation medium
Sterilising conditions are 0.15MPa, 121 DEG C of sterilizing 30min.
(4) biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and turf are poured into mixer and is adsorbed, mixed
After closing uniformly, the rotten material of agricultural crop straw, diatomite and white carbon mineral matter and desulfurated plaster, stirring are progressively added toward mixer
Material is crushed after uniform, crossed 100 mesh sieves, adjusted material moisture and pH, obtained final product based on charcoal and Methylotrophic
The bio-organic fertilizer of bacillus, wherein the group in the bio-organic fertilizer based on charcoal and Methylotrophic bacillus
Point, by weight percentage, including biomass charcoal powder 3%, microbial function bacterium 5%, turf 5%, mineral matter 13%, desulfurization stone
Cream 10%, remaining is the rotten material of agricultural crop straw, and the total amount of above mass percentage content is 100%.
Embodiment 5：
(1) biomass material peanut shell and shrub branch are ground into powder, are placed in resistant to elevated temperatures closed container,
500 DEG C, carry out carbonization treatment 2.5h under anoxia condition, crushed after being cooled to room temperature, obtain diameter less than 0.25mm, proportion is
1.1g/cm3Biomass charcoal powder.
(2) by agricultural crop straw longan branches and leaves and the rotten fermentation of maize straw heap, water content is 59%, and temperature is 45 DEG C, when
Between be 8d, cross 80 mesh sieves, form the rotten material of agricultural crop straw.
(3) the Methylotrophic Bacillus strain that will be stored under -4 DEG C of environment is seeded on beef peptone flat board,
30 DEG C of culture 48h, jump goes single bacterium colony to be seeded in beef extract-peptone fluid nutrient medium, and inoculation liquid is obtained after 30 DEG C of culture 24h,
Inoculation liquid is inoculated in the 300L seeding tanks equipped with seed culture medium, it is 10% to connect bacterium amount, is fermented after connecing bacterium, initial pH
Value 7.1,30 DEG C of temperature, rotating speed 220r/min, as seeding tank fermentation 9h, thalli growth is in logarithmic phase, now by seeding tank
Zymotic fluid be added to equipped with fermentation medium 3000L fermentation tanks relaying supervention ferment, the same seeding tank of control condition, cultivate 30h,
When thalline sporulation 92%, terminate fermentation, it is 26.4% to obtain moisture, and pH value is 7.0 Methylotrophic gemma
Zymotic fluid, the parameter of wherein beef extract-peptone fluid nutrient medium is：Beef extract 5g, peptone 10g, agar 15-20g, pH
7.2-7.6, the parameter of the seed culture medium is：Glucose 10g/L, peptone 2g/L, MgSO4 0.5g/L, yeast extract 2g/
L, beef extract 2g/L, the parameter of fermentation medium is：Corn flour 30g/L, beancake powder 20g/L, MgSO40.5g/L, NaH2PO4
0.5g/L, CaCl22H2O 1g/L, K2HPO40.5g/L, beef extract-peptone fluid nutrient medium, seed culture medium and fermentation training
The sterilising conditions for supporting base are 0.11MPa, 121 DEG C of sterilizing 30min.
(4) biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and turf are poured into mixer and is adsorbed, mixed
After closing uniformly, agricultural crop straw corruption material, zeolite powder, white carbon, precipitated calcium carbonate mineral matter are progressively added toward mixer and is taken off
Sulphur gypsum, is crushed material after stirring, and crosses 100 mesh sieves, adjusts material moisture and pH, obtain final product based on charcoal and
The bio-organic fertilizer of Methylotrophic bacillus, wherein the biological organic based on charcoal and Methylotrophic bacillus
Component in fertilizer, by weight percentage, including biomass charcoal powder 4%, microbial function bacterium 6%, turf 8%, mineral matter
16%th, desulfurated plaster 13%, remaining is the rotten material of agricultural crop straw, and the total amount of above mass percentage content is 100%.
Embodiment 6：
(1) biomass material wheat stalk and peanut shell powder are broken into the powder of 0.5mm, resistant to elevated temperatures closed container is placed in
In, carbonization treatment 1.5h is carried out under 600 DEG C, anoxia condition, crushed after being cooled to room temperature, diameter is obtained less than 0.5mm, proportion
It is 0.8g/cm3Biomass charcoal powder.
(2) by agricultural crop straw cassava stalk and the rotten fermentation of pineapple stem and leaf heap, water content is 59%, and temperature is 45 DEG C, when
Between be 13d, cross 80 mesh sieves, form the rotten material of agricultural crop straw.
(3) the Methylotrophic Bacillus strain that will be stored under -4 DEG C of environment is seeded on beef peptone flat board,
30 DEG C of culture 48h, jump goes single bacterium colony to be seeded in beef extract-peptone fluid nutrient medium, and inoculation liquid is obtained after 30 DEG C of culture 24h,
Inoculation liquid is inoculated in the 300L seeding tanks equipped with seed culture medium, it is 10% to connect bacterium amount, is fermented after connecing bacterium, initial pH
Value 6.9,30 DEG C of temperature, rotating speed 200r/min, as seeding tank fermentation 9.5h, thalli growth is in logarithmic phase, now by seeding tank
In zymotic fluid be added to equipped with fermentation medium 3000L fermentation tanks relaying supervention ferment, the same seeding tank of control condition, culture
28-30h, as thalline sporulation 90-95%, terminates fermentation, and it is 27.4% to obtain moisture, and pH value is 7.3 methyl
Auxotype gemma zymotic fluid, the parameter of wherein beef extract-peptone fluid nutrient medium is：Beef extract 5g, peptone 10g, agar
18g, pH 7.4, the parameter of the seed culture medium is：Glucose 10g/L, peptone 2g/L, MgSO4 0.5g/L, yeast extract
2g/L, beef extract 2g/L, the parameter of fermentation medium is：Corn flour 30g/L, beancake powder 20g/L, MgSO40.5g/L,
NaH2PO40.5g/L, CaCl22H2O1g/L, K2HPO40.5g/L, beef extract-peptone fluid nutrient medium, seed culture medium
It is 0.15MPa, 121 DEG C of sterilizing 30min with the sterilising conditions of fermentation medium.
(4) biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and turf are poured into mixer and is adsorbed, mixed
After closing uniformly, agricultural crop straw corruption material, zeolite powder, vermiculite power, diatomite, white carbon, lightweight carbon are progressively added toward mixer
Sour calcium mineral matter and desulfurated plaster, are crushed material after stirring, and cross 100 mesh sieves, adjust material moisture and pH, i.e.,
The bio-organic fertilizer of charcoal and Methylotrophic bacillus must be based on, wherein based on charcoal and Methylotrophic gemma
Component in the bio-organic fertilizer of bacillus, by weight percentage, including biomass charcoal powder 3%, microbial function bacterium 8%,
Turf 7%, mineral matter 14%, desulfurated plaster 13%, remaining is the rotten material of agricultural crop straw, the total amount of above mass percentage content
It is 100%.
Take 3 consistent leaf Muskmelon Seedlings of growing way to be transplanted in basin alms bowl, after transplanting 7 days, muskmelon seedling is carried out to connect bacterium treatment, first
Apply the bio-organic fertilizer based on charcoal and Methylotrophic bacillus in embodiment 1-6, droop was inoculated with every 1 day
Pathogen, after connecing bacterium 7 days, records muskmelon incidence, calculates its disease index and prevention effect.
Comparative example 1：First be inoculated with Pathogen of Fusarium Wilt, every 1 day apply embodiment 1 in based on charcoal and Methylotrophic
The bio-organic fertilizer of bacillus, after connecing bacterium 7 days, records muskmelon incidence, calculates its disease index and prevention effect.
Comparative example 2：Only it is inoculated with Pathogen of Fusarium Wilt.
Wherein incidence is divided into 5 grades according to standard：0 grade of complete stool is disease-free, and 1 grade of plant blade face below 1/4 shows wilting disease
Shape, 2 grades of blade faces of plant 1/4 to 1/2 show wilting symptom, and 3 grades of blade faces of plant more than 1/2 show wilting symptom, and 4 grades of complete stools are wilted
It is dead.
Disease index=[∑ (state of an illness strain number × disease series)/(representative of the treatment Potted orchard summation × morbidity most heavy duty
Numerical value)] × 100.
With respect to prevention effect=[(control disease index-treatment disease index)/control disease index] × 100%.
After testing, embodiment 1-6 prepare the bio-organic fertilizer based on charcoal and Methylotrophic bacillus with
And prior art to droop to prevent and treat result as follows：
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | Reference examples 1 | Reference examples 2 | |
| Disease index | 37.5 | 32.5 | 36.5 | 35 | 34 | 35.5 | 40 | 85 |
| Prevention effect (%) | 62 | 75 | 68 | 70 | 68 | 69 | 52 | -- |
As seen from the above table, the bio-organic fertilizer pair based on charcoal and Methylotrophic bacillus that prepared by the present invention
The regulation effect of withered grass germ is notable, also with certain prevention effect.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
The personage for knowing this technology all can carry out modifications and changes under without prejudice to spirit and scope of the invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete with institute under technological thought without departing from disclosed spirit such as
Into all equivalent modifications or change, should be covered by claim of the invention.
Claims (10)
1. a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus, it is characterised in that：The biological organic
Fertilizer includes the rotten material of biomass charcoal powder, microbial function bacterium, turf, agricultural crop straw, mineral matter and desulfurated plaster, the biomass
The raw material of powdered carbon is in wheat stalk, maize straw, rice straw, cotton stalk, peanut shell, fruit tree pruning, shrub branch
One or more of mixture, the microbial function bacterium is Methylotrophic fermentation of bacillus liquid, the agricultural crop straw
The raw material of corruption material is the one kind or many in cassava stalk, sugarcane cauline leaf, mango branches and leaves, longan branches and leaves, maize straw, pineapple stem and leaf
The mixture planted, the mineral matter is one or more in zeolite powder, vermiculite power, diatomite, white carbon, precipitated calcium carbonate
Mixture.
2. a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus according to claim 1, it is special
Levy and be：Component in the biological organic fertilizer based on charcoal and Methylotrophic bacillus, by weight percentage,
Including biomass charcoal powder 3-5%, microbial function bacterium 5-10%, turf 5-10%, mineral matter 13-20%, desulfurated plaster 10-
15%, remaining is the rotten material of agricultural crop straw, and the total amount of above mass percentage content is 100%.
3. a kind of preparation method of the biological organic fertilizer based on charcoal and Methylotrophic bacillus, it is characterised in that bag
Include following steps：
(1) biomass material is ground into powder, is placed in resistant to elevated temperatures closed container, entered under 300-700 DEG C, anoxia condition
Row carbonization treatment 1-3h, crushes after being cooled to room temperature, obtains biomass charcoal powder；
(2) by the rotten fermentation of agricultural crop straw powder heap, 60-80 mesh sieves are crossed, forms the rotten material of agricultural crop straw；
(3) Methylotrophic bacillus is formed into Methylotrophic fermentation of bacillus liquid through inoculation, culture, fermentation；
(4) biomass charcoal powder, Methylotrophic fermentation of bacillus liquid and the turf for preparing step (1) pour into mixer
Row absorption, after being well mixed, the rotten material of agricultural crop straw, mineral matter and the desulfurization of step (2) preparation is progressively added toward mixer
Gypsum, is crushed material after stirring, and crosses 100 mesh sieves, adjusts material moisture and pH, is obtained final product based on charcoal and first
The bio-organic fertilizer of base auxotype bacillus.
4. the preparation of a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus according to claim 3
Method, it is characterised in that：In the step (1), the particle diameter of biomass charcoal powder is less than 1mm.
5. the preparation of a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus according to claim 3
Method, it is characterised in that：In the step (2), the condition of the rotten fermentation of heap is：Water content is 55-60%, and temperature is 40-65 DEG C,
Time is 7-15d.
6. the preparation of a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus according to claim 3
Method, it is characterised in that：In the step (3), the specific preparation method of Methylotrophic fermentation of bacillus liquid is：To preserve
Methylotrophic Bacillus strain under -4 DEG C of environment is seeded on beef peptone flat board, and list is removed in 30 DEG C of culture 48h, jump
In colony inoculation to beef extract-peptone fluid nutrient medium, inoculation liquid is obtained after 30 DEG C of culture 24h, inoculation liquid is inoculated in and is equipped with
In the 300L seeding tanks of seed culture medium, it is 10% to connect bacterium amount, is fermented after connecing bacterium, initial pH value 6.9-7.1, temperature 28-
35 DEG C, rotating speed 180-220r/min, as seeding tank fermentation 9-10h, thalli growth is in logarithmic phase, now by the hair in seeding tank
Zymotic fluid is added to the 3000L fermentation tanks relaying supervention ferment equipped with fermentation medium, and the same seeding tank of control condition cultivates 28-30h,
As thalline sporulation 90-95%, terminate fermentation, obtain Methylotrophic gemma zymotic fluid.
7. the preparation of a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus according to claim 6
Method, it is characterised in that：The parameter of the beef extract-peptone fluid nutrient medium is：Beef extract 5g, peptone 10g, agar 15-
20g, pH 7.2-7.6, the parameter of the seed culture medium is：Glucose 10g/L, peptone 2g/L, MgSO4 0.5g/L, ferment
Female cream 2g/L, beef extract 2g/L, the parameter of the fermentation medium is：Corn flour 30g/L, beancake powder 20g/L, MgSO4
0.5g/L, NaH2PO40.5g/L, CaCl22H2O 1g/L, K2HPO4 0.5g/L。
8. the preparation of a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus according to claim 6
Method, it is characterised in that：The sterilising conditions of the beef extract-peptone fluid nutrient medium, seed culture medium and fermentation medium are
0.1-0.15MPa, 121 DEG C of sterilizing 30min.
9. the preparation of a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus according to claim 3
Method, it is characterised in that：In the step (3), moisture≤29% of Methylotrophic gemma bacillus agent, pH value is
6.9-7.5。
10. the system of a kind of biological organic fertilizer based on charcoal and Methylotrophic bacillus according to claim 3
Preparation Method, it is characterised in that：In the step (4), the bio-organic fertilizer based on charcoal and Methylotrophic bacillus
In component, by weight percentage, including biomass charcoal powder 3-5%, microbial function bacterium 5-10%, turf 5-10%, ore deposit
Material 13-20%, desulfurated plaster 10-15%, remaining is the rotten material of agricultural crop straw, and the total amount of above mass percentage content is
100%.
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Application publication date: 20170623 |