CN107955616A - A kind of preparation method suitable for harmful microorganism soil conditioner - Google Patents

A kind of preparation method suitable for harmful microorganism soil conditioner Download PDF

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CN107955616A
CN107955616A CN201711049378.0A CN201711049378A CN107955616A CN 107955616 A CN107955616 A CN 107955616A CN 201711049378 A CN201711049378 A CN 201711049378A CN 107955616 A CN107955616 A CN 107955616A
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preparation
microorganism
soil conditioner
charcoal
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任倩妍
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Pujiang County Hong Hung Horticulture Research And Development Co Ltd
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Pujiang County Hong Hung Horticulture Research And Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • AHUMAN NECESSITIES
    • A62LIFE-SAVING; FIRE-FIGHTING
    • A62DCHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • AHUMAN NECESSITIES
    • A62LIFE-SAVING; FIRE-FIGHTING
    • A62DCHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20Organic substances
    • AHUMAN NECESSITIES
    • A62LIFE-SAVING; FIRE-FIGHTING
    • A62DCHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/40Inorganic substances
    • A62D2101/43Inorganic substances containing heavy metals, in the bonded or free state
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use

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  • Molecular Biology (AREA)
  • Soil Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Business, Economics & Management (AREA)
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  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
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Abstract

The invention discloses a kind of preparation method suitable for harmful microorganism soil conditioner, including:The preparation of charcoal, the modification of charcoal, fermentation, compounding modifying agent, soil conditioner have the material composition of following parts by weight:Low temperature drying after 30 50 parts of modification biological charcoals, 100 150 parts of maize straw fermentates, 60 80 parts of sludge, 15 35 parts of desulfurated plaster mixing, 60 100 mesh sieves are crossed after crushing, 2 10 parts of microorganism formulations, microorganism accelerating agent, the mixture of enzyme preparation are added in outsifting.Have the beneficial effect that:Soil conditioner can effectively suppress soil harmful microbe growth and breeding, enzyme preparation and the secretory substance such as part of enzyme, hormone, ketone etc. of microorganism formulation or its further catabolite have allelopathy at the same time, allelopathy can further suppress harmful microbe procreation growth in soil, fundamentally improve the structure of harmful microorganism contaminated soil.

Description

A kind of preparation method suitable for harmful microorganism soil conditioner
Technical field
The present invention relates to soil conditioner field, more particularly, to a kind of system suitable for harmful microorganism soil conditioner Preparation Method.
Technical background
Edaphon pollution refers to that one or several harmful biotic populations are simultaneously a large amount of numerous from external environment intrusion soil Grow, cause soil quality to decline, not only break up the original ecological balance, can also be to animals and plants and health and the ecosystem Cause harmful effect.
As China is a large agricultural country, fertilizer demand amount is huge, and the traditional concept of people is considered as sanitary sewage and contains There is a rare nutrient, and human and animal excreta is even more containing abundant organic nutrition, besides everybody thinks that crops can be with Purify sewage.Therefore, sewage irrigation and excrement fertilising are always a big feature of China's agricultural.However, sanitary sewage contains greatly Bacterium is measured, industry and hospital wastewater are even more to be rich in various pathogen.In addition data shows, sludge, rubbish and muck all may Carry a large amount of pathogenic microorganisms and parasitic ovum.Many peasants are accustomed to the poultry died of illness to bury, these viral corpse It is a big source for pathogenic bacteria in soil, easily causes geobiont to pollute and expand the propagation of disease.In addition, things is all general All over contact, the microorganism in atmospheric thermodynamics and hydrosphere can also cause biological pollution into soil.In nature, soil is micro- life Thing inhabites the environmental optima of growth.There is species is various, various microorganisms of enormous amount in soil, they constitute one Metastable ecological community.The harmful microbial population of one or more, people's soil and wherein a large amount of procreations are invaded from the external world, Original dynamic equilibrium can be destroyed, harmful effect is produced to the mankind or the ecosystem.Undressed excrement, rubbish, city Sanitary sewage and feed lot and the dirt in slaughterhouse etc., can bring harmful microbial population into, cause soil pollution.Its In, sterile-processed sewage is come with infectious hospital and dirt harm is most strong.They not only endanger human health, also seriously Plant is endangered, causes the agriculture underproduction.Such as certain plants pathogenic bacteria intrusion soil after, can cause tomato, eggplant, capsicum, The bacterial wilt of plant, can also cause the bacillary of fruit tree beyond the over one hundred kind of plant of Solanaceae such as potato, tobacco, belladonna and Solanaceae Ulcer and root knot.Some pathomycetes can then cause the kind of Chinese cabbage, rape, leaf mustard, radish, wild cabbage, shepherd's purse etc. more than 100 to be planted Training and the clubroot of wild brassicaceous vegetable, can also induce the droop of the various plants such as eggplant, cotton, cucumber, watermelon, dish The root rot of beans, cowpea etc., and wheat, barley, oat, jowar, corn, the smut etc. of millet.Prevent the micro- of soil Biological pollution, it is necessary to which harmless processing is carried out to pollution sources.And by studying contaminating microorganisms in soil and the ecosystem Migration, distribution and Fluctuation, take corresponding control measure.
The content of the invention
It is an object of the invention to provide a kind of preparation method suitable for harmful microorganism soil conditioner, this preparation side Method is simple and practicable, of low cost, Environmental Safety, and soil conditioner has harmful microorganism flourish in preferable suppression soil Function, while edaphon structure can also be improved.
Microorganism and its bought enterprise used are in the method for the present invention:Photosynthetic bacteria, the gloomy biotechnology of Cangzhou Lay have Limit company;Bacillus subtilis, actinomyces, saccharomycete, photosynthetic bacteria, lactic acid bacteria, Cangzhou Yi Hong animal health-care products Co., Ltd; Bifidobacterium Bifidum, Beijing Chuan Xiu Science and Technology Ltd.s;Acetic acid bacteria, Jining Yu Yuan bio tech ltd;Penicillium notatum, verdigris are false single Born of the same parents bacterium, Nanjing Bian Zhen bio tech ltd;Micrococcus luteus, Huankai Microbes Tech Co., Ltd., Guangdong.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is:One kind is suitable for harmful microorganism The preparation method of soil conditioner, including:The preparation of charcoal, the modification of charcoal, fermentation, compounding modifying agent, specifically include Following steps:
The preparation of charcoal:Using water plant as raw material, through crushing, sieve, spare after natural air drying;With auto-programming heating furnace Bamboo scraps are carbonized, 580-600 DEG C is warming up to the speed of 5-8 DEG C/min, and keep the temperature 2.5-3.0 it is small when;By natural cooling When charcoal afterwards is small with the sodium hydroxide solution immersion 24-30 of 2-3mol/L, is then heated 8-10 minutes, inclined with high fiery microwave Solution is removed, then adds deionized water microwave heating cleaning 5-10 minutes, it is stand-by with 180-190 DEG C of drying after so washing 2-3; Activation causes biological carbon surface and hole occurs in inside, and specific surface area expands inside charcoal, is the further modified of charcoal More sites are provided;
The modification of charcoal:Weigh dried charcoal to be put into 15-20 times of concentrated hydrochloric acid, be sufficiently mixed, stir 2-2.5 Hour after, be put into reaction kettle at 180-200 DEG C heat 15-18 it is small when, then clean 4-5 times after 100- with deionized water 105 DEG C of drying, that is, obtain modification biological charcoal;The activation of acid causes charcoal inner void further to increase, and specific surface area is into one Step expands, and is conducive to provide more adsorption sites, charcoal is not only acted as the effect of carbon sequestration, water holding, moreover it is possible to increase soil Nutrient withholds, reduces the soil weight, increase porosity;Charcoal Pyrogentisinic Acid, 2,4- chlorophenesic acids, p-nitrophenol, aminobenzene The phenolic comp ' ds pollutions such as phenol are respectively provided with higher adsorption rate;
Fermentation:Maize straw is smashed the stalk section that growth degree is 1-2cm, is watered with water, it is 60-70% to control water content, by straw Stalk leavening is sprinkled into inside stalk, and the usage amount of stover ferment agent is the 5-8 ‰, natural temperature, nature pH of stalk weight, per 2- Turning in 3 days once, ferments 15-18 days up to maize straw fermentate;Maize straw fermentate can be obviously improved soil physical chemistry Matter, effectively improves chemical fertilizer utilization ratio, the amount of application of less chemical fertilizer, simultaneously because maize straw fermentate improves crop rhizosphere soil Earth permeability, can be effectively improved tree characteristics, enhance plant pair harmful microbe antagonism, inhibit soil indirectly Middle harmful microbe growth and breeding;
Compound modifying agent:By 30-50 parts of modification biological charcoals, 100-150 parts of maize straw fermentates, 60-80 parts of sludge, 15-35 Low temperature drying after the mixing of part desulfurated plaster, crosses 60-100 mesh sieves after crushing, 2-10 parts of microorganism formulations, micro- are added in outsifting The mixture of Biostimulation agent, enzyme preparation, is to be suitable for harmful microbe soil conditioner after mixing;Microorganism promotees It is a kind of functional polypeptide into agent, the amino acid sequence of functional polypeptide is: SCASRCCRAGYYKSRCRARRCRARRCGCRARRCGYYVSVFRC;Microorganism accelerating agent can be in chemical pollution soil Indigenous bacterium is stimulated, and stimulates the secretion of indigenous bacterium enzyme and the activity of enzyme, promotes its breeding and growth, to accelerate microbial physiology Activity;Soil conditioner can improve soil texture, improve retentiveness, the porosity of soil, strengthen the fertilizer-preserving ability of soil, adjust The pH value of soil is saved, changes harmful microbe breeding environment, so as to suppress its growth breeding;Microorganism in modifying agent at the same time Preparation can make the concentration of rape endophytic bacterial hyphal cell shorten, and cell wall rupture, plasm is excessive, so as to suppress fungi growth hair Educate and sclerotial germination, enzyme preparation and the secretory substance such as part of enzyme, hormone, ketone etc. of microorganism formulation or its is further Catabolite there is allelopathy, the growth of pathogen can be suppressed, matter and energy is obtained by nitrification to some Germ also have preferable inhibition.
Preferably, the water plant in the preparation process of charcoal is calamus or fishscale bamboo or reed or Wang Lian.
Preferably, the stover ferment agent in fermentation step is bacillus subtilis or lactic acid bacteria or Bifidobacterium Bifidum or yeast Bacterium or actinomyces or zytase or acetic acid bacteria.
Preferably, the weight ratio of microorganism formulation, microorganism accelerating agent, enzyme preparation is 12-15:1:3-5.
Preferably, microorganism formulation is bacillus subtilis, photosynthetic bacteria, Penicillium notatum, pseudomonas aeruginosa, gamboge are micro- The mixture that at least three kinds of coccus.
Preferably, enzyme preparation is hemicellulase, tannase, polyphenol oxidase, lipase, protease, glutathione At least two mixture in S- transferases.
Compared with prior art, the advantage of the invention is that:1)The method of the present invention uses water plant, maize straw, dirt The discarded object production of the industrial and agricultural productions such as mud, desulfurated plaster prepares soil conditioner, not only reduces production cost, and protect Environment, to realize turn waste into wealth;2)Soil conditioner can effectively improve soil texture, strengthen soil water retention and hole Degree, strengthens the fertilizer-preserving ability of soil, while has abundant effect to the microorganism in soil, is effectively improved the Tiny ecosystem knot of soil Structure;3)Soil conditioner not only has the function that to reduce heavy metal in soil content, degradable organic pollutant, in soil conditioner Enzyme preparation and the secretory substance such as part of enzyme, hormone, ketone etc. of microorganism formulation or its further catabolite With allelopathy, allelopathy can further suppress harmful microbe procreation growth in soil, fundamentally improve The structure of harmful microorganism contaminated soil.
Embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
A kind of preparation method suitable for harmful microorganism soil conditioner, comprises the following steps:
1)Using calamus as raw material, through crushing, sieve, spare after natural air drying;Bamboo scraps are carbonized with auto-programming heating furnace, Be warming up to 580 DEG C with the speed of 5 DEG C/min, and keep the temperature 2.5 it is small when;By the hydroxide of the charcoal 2mol/L after natural cooling When sodium solution immersion 24 is small, then heated 8 minutes with high fiery microwave, incline solution, then adds deionized water microwave heating cleaning 5 Minute, it is stand-by with 180 DEG C of drying so after washing 2;2)Weigh dried charcoal to be put into 15 times of concentrated hydrochloric acid, fill Point mixing, when stirring 2 is small after, be put into reaction kettle at 180 DEG C heating 15 it is small when, then with deionized water clean 4 times after 100-105 DEG C of drying, that is, obtain modification biological charcoal;3)Maize straw is smashed the stalk section that growth degree is 1cm, is watered with water, It is 60% to control water content, and the bacillus subtilis of stalk weight 5 ‰ is sprinkled into inside stalk, natural temperature, nature pH, every 2 days Turning once, ferments 15 days up to maize straw fermentate;4)By 30 parts of modification biological charcoals, 100 parts of maize straw fermentates, 60 Low temperature drying after part sludge, 15 parts of desulfurated plasters mixing, crosses 60 mesh sieves, 0.4 part of withered grass gemma is added in outsifting after crushing Bacillus, 0.5 part of photosynthetic bacteria, 0.5 portion of Penicillium notatum, 0.1 part of microorganism accelerating agent, 0.3 part of tannase, 0.2 part of polyphenol oxidase, It is to be suitable for harmful microbe soil conditioner after mixing.
Implement 2:
A kind of preparation method suitable for harmful microorganism soil conditioner, comprises the following steps:
1)The preparation of charcoal:Using fishscale bamboo as raw material, through crushing, sieve, spare after natural air drying;With auto-programming heating furnace pair Bamboo scraps are carbonized, and 600 DEG C are warming up to the speed of 8 DEG C/min, and keep the temperature 3.0 it is small when;Charcoal after natural cooling is used When the sodium hydroxide solution immersion 30 of 3mol/L is small, then heated 10 minutes with high fiery microwave, incline solution, then adds deionized water It is stand-by with 190 DEG C of drying so after washing 3 with microwave heating cleaning 5-10 minutes;Activation causes biological carbon surface and inside There is hole, specific surface area expands inside charcoal, and more sites are provided for the further modification of charcoal;
2)The modification of charcoal:Weigh dried charcoal to be put into 20 times of concentrated hydrochloric acid, be sufficiently mixed, stirring 2.5 is small Shi Hou, be put into reaction kettle at 200 DEG C heating 18 it is small when, then clean 5 times with deionized water and is dried after 105 DEG C, that is, obtain Modification biological charcoal;The activation of acid causes charcoal inner void further to increase, and specific surface area further expands, and is conducive to provide More adsorption sites, make charcoal not only act as the effect of carbon sequestration, water holding, moreover it is possible to increase soil nutrient and withhold, reduce soil Unit weight, increase porosity;The phenolic comp ' ds pollutions such as charcoal Pyrogentisinic Acid, 2,4- chlorophenesic acids, p-nitrophenol, amino-phenol have There is higher adsorption rate;
3)Fermentation:Maize straw is smashed the stalk section that growth degree is 2cm, is watered with water, it is 70% to control water content, adds straw The lactic acid bacteria of stalk weight 3 ‰, 5 ‰ Bifidobacterium Bifidum, natural temperature, nature pH, every turning in 3 days once, ferment 18 days up to beautiful Rice stalk fermentation thing;Maize straw fermentate can be obviously improved soil physico-chemical property, effectively improve chemical fertilizer utilization ratio, less chemical fertilizer Amount of application, simultaneously because maize straw fermentate improves crop rhizosphere soils permeability, tree characteristics can be effectively improved, Plant pair harmful microbe antagonism is enhanced, inhibits harmful microbe growth and breeding in soil indirectly;
4)Compound modifying agent:50 parts of modification biological charcoals, 150 parts of maize straw fermentates, 80 parts of sludge, 35 parts of desulfurated plasters are mixed Low temperature drying after conjunction, crosses 100 mesh sieves after crushing, 5 parts of photosynthetic bacterias, 1.5 portions of Penicillium notatums, 1 part of verdigris vacation are added in outsifting Monad, 0.5 part of microorganism accelerating agent, 1 part of lipase, 0.5 part of protease, the mixture of 0.5 part of glutathione S-transferase, It is to be suitable for harmful microbe soil conditioner after mixing;Microorganism accelerating agent is a kind of functional polypeptide, function The amino acid sequence of property polypeptide is:SCASRCCRAGYYKSRCRARRCRARRCGCRARRCGYYVSVFRC;Microorganism accelerating agent Indigenous bacterium in chemical pollution soil can be stimulated, stimulate the secretion of indigenous bacterium enzyme and the activity of enzyme, promote its breeding With growth, to accelerate microbial physiology movable;Soil conditioner can improve soil texture, improve retentiveness, the hole of soil Degree, strengthens the fertilizer-preserving ability of soil, adjusts the pH value of soil, changes harmful microbe breeding environment, so as to suppress its growth Breeding;The microorganism formulation in modifying agent can make the concentration of rape endophytic bacterial hyphal cell shorten at the same time, and cell wall rupture is primary Matter is excessive, so as to suppress fungal growth and development and sclerotial germination, the secretory substance of enzyme preparation and microorganism formulation for example enzyme, hormone, A part for ketone etc. or its further catabolite have allelopathy, can suppress the growth of pathogen, to some The germ for obtaining matter and energy by nitrification also has preferable inhibition.
Implement 3:
A kind of preparation method suitable for harmful microorganism soil conditioner, including:The preparation of charcoal, the modification of charcoal, Fermentation, compounding modifying agent, specifically include following steps:
The preparation of charcoal:Using reed as raw material, through crushing, sieve, spare after natural air drying;With auto-programming heating furnace to bamboo Bits are carbonized, and 590 DEG C are warming up to the speed of 6 DEG C/min, and keep the temperature 2.5 it is small when;Charcoal after natural cooling is used When the sodium hydroxide solution immersion 24 of 2.5mol/L is small, then heated 8 minutes with high fiery microwave, incline solution, then adds deionization Water microwave heating cleaning 8 minutes, it is stand-by with 180 DEG C of drying so after washing 3;Activation causes biological carbon surface and inside to go out Existing hole, charcoal inside specific surface area expand, and more sites are provided for the further modification of charcoal;
The modification of charcoal:Weigh dried charcoal to be put into 16 times of concentrated hydrochloric acid, be sufficiently mixed, stirring 2 it is small when after, Be put into reaction kettle at 180 DEG C heating 15 it is small when, then clean 4 times after 100 DEG C of drying with deionized water, that is, obtains being modified life Thing charcoal;The activation of acid causes charcoal inner void further to increase, and specific surface area further expands, and is conducive to provide more Adsorption site, makes charcoal not only act as the effect of carbon sequestration, water holding, moreover it is possible to increases soil nutrient and withholds, reduce the soil weight, increase Macroporsity;The phenolic comp ' ds pollutions such as charcoal Pyrogentisinic Acid, 2,4- chlorophenesic acids, p-nitrophenol, amino-phenol are respectively provided with higher Adsorption rate;
Fermentation:Maize straw is smashed the stalk section that growth degree is 1.5cm, is watered with water, it is 65% to control water content, adds straw The saccharomycete of stalk weight 6 ‰, natural temperature, nature pH, every turning in 3 days once, ferment 16 days up to maize straw fermentate;It is beautiful Rice stalk fermentation thing can be obviously improved soil physico-chemical property, effectively improve chemical fertilizer utilization ratio, the amount of application of less chemical fertilizer, while by Crop rhizosphere soils permeability is improved in maize straw fermentate, tree characteristics can be effectively improved, enhancing plant pair has The antagonism of evil microorganism, inhibits harmful microbe growth and breeding in soil indirectly;
Compound modifying agent:40 parts of modification biological charcoals, 120 parts of maize straw fermentates, 70 parts of sludge, 20 parts of desulfurated plasters are mixed Low temperature drying afterwards, crosses 80 mesh sieves after crushing, 2.2 parts of bacillus subtilises, 1 part of photosynthetic bacteria, 1 part of mould are added in outsifting Bacterium, 0.3 part of microorganism accelerating agent, 0.5 part of tannase, the mixture of 1 part of lipase, be after mixing be suitable for it is harmful micro- The soil conditioner of biology;Microorganism accelerating agent is a kind of functional polypeptide, and the amino acid sequence of functional polypeptide is: SCASRCCRAGYYKSRCRARRCRARRCGCRARRCGYYVSVFRC;Microorganism accelerating agent can be in chemical pollution soil Indigenous bacterium is stimulated, and stimulates the secretion of indigenous bacterium enzyme and the activity of enzyme, promotes its breeding and growth, to accelerate microbial physiology Activity;Soil conditioner can improve soil texture, improve retentiveness, the porosity of soil, strengthen the fertilizer-preserving ability of soil, adjust The pH value of soil is saved, changes harmful microbe breeding environment, so as to suppress its growth breeding;Microorganism in modifying agent at the same time Preparation can make the concentration of rape endophytic bacterial hyphal cell shorten, and cell wall rupture, plasm is excessive, so as to suppress fungi growth hair Educate and sclerotial germination, enzyme preparation and the secretory substance such as part of enzyme, hormone, ketone etc. of microorganism formulation or its is further Catabolite there is allelopathy, the growth of pathogen can be suppressed, matter and energy is obtained by nitrification to some Germ also have preferable inhibition.
Routine operation in operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that the above is only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Sequence table
<110>Close big vast gardening research and development Co., Ltd in Pujiang County
<120>A kind of preparation method suitable for harmful microorganism soil conditioner
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 42
<212> PRT
<213>Artificial synthesized (Mytilus coruscus)
<400> 1
Ser Cys Ala Ser Arg Cys Cys Arg Ala Gly Tyr Tyr Lys Ser Arg Cys
1 5 10 15
Arg Ala Arg Arg Cys Arg Ala Arg Arg Cys Gly Cys Arg Ala Arg Arg
20 25 30
Cys Gly Tyr Tyr Val Ser Val Phe Arg Cys
35 40

Claims (9)

1. a kind of preparation method suitable for harmful microorganism soil conditioner, including:The preparation of charcoal, charcoal change Property, fermentation, compounding modifying agent, it is characterised in that:It is described compounding modifying agent step be:By 30-50 parts of modification biological charcoals, 100- Low temperature drying after 150 parts of maize straw fermentates, 60-80 parts of sludge, 15-35 parts of desulfurated plaster mixing, crosses 60-100 after crushing Mesh sieve, 2-10 parts of microorganism formulations, microorganism accelerating agent, the mixture of enzyme preparation are added in outsifting, is after mixing For suitable for harmful microbe soil conditioner.
A kind of 2. preparation method suitable for harmful microorganism soil conditioner according to claim 1, it is characterised in that: Microorganism formulation, microorganism accelerating agent in the compounding modifying agent step, the weight ratio of enzyme preparation are 12-15:1:3-5.
A kind of 3. preparation method suitable for harmful microorganism soil conditioner according to claim 1, it is characterised in that: It is described compounding modifying agent step in microorganism formulation for bacillus subtilis, photosynthetic bacteria, Penicillium notatum, pseudomonas aeruginosa, The mixture that at least three kinds of micrococcus luteus.
A kind of 4. preparation method suitable for harmful microorganism soil conditioner according to claim 1, it is characterised in that: Microorganism accelerating agent in the compounding modifying agent step is a kind of functional polypeptide, and the amino acid sequence of functional polypeptide is: SCASRCCRAGYYKSRCRARRCRARRCGCRARRCGYYVSVFRC。
A kind of 5. preparation method suitable for harmful microorganism soil conditioner according to claim 1, it is characterised in that: It is described compounding modifying agent step in enzyme preparation be:Hemicellulase, tannase, polyphenol oxidase, lipase, protease, paddy At least two mixture in the sweet peptide S- transferases of Guang.
A kind of 6. preparation method suitable for harmful microorganism soil conditioner according to claim 1, it is characterised in that: The preparation process of the charcoal is:Using water plant as raw material, through crushing, sieve, spare after natural air drying;With auto-programming Heating furnace carbonizes bamboo scraps, and 580-600 DEG C is warming up to the speed of 5-8 DEG C/min, and keep the temperature 2.5-3.0 it is small when;Will be certainly When right charcoal after cooling is small with the sodium hydroxide solution immersion 24-30 of 2-3mol/L, then with high fiery microwave heating 8-10 Minute, incline solution, then adds deionized water microwave heating cleaning 5-10 minutes, after so washing 2-3, with 180-190 DEG C of baking It is dry, it is stand-by.
A kind of 7. preparation method suitable for harmful microorganism soil conditioner according to claim 1, it is characterised in that: The modification procedure of the charcoal is:Weigh dried charcoal to be put into 15-20 times of concentrated hydrochloric acid, be sufficiently mixed, stir Mix 2-2.5 it is small when after, be put into reaction kettle at 180-200 DEG C heat 15-18 it is small when, after then being cleaned 4-5 times with deionized water In 100-105 DEG C of drying, that is, obtain modification biological charcoal.
A kind of 8. preparation method suitable for harmful microorganism soil conditioner according to claim 1, it is characterised in that: The fermentation step is:Maize straw is smashed the stalk section that growth degree is 1-2cm, is watered with water, it is 60- to control water content 70%, stover ferment agent is sprinkled into inside stalk, the usage amount of stover ferment agent is the 5-8 ‰ of stalk weight, natural temperature, from Right pH, per turning in 2-3 days once, ferments 15-18 days up to maize straw fermentate.
A kind of 9. preparation method suitable for harmful microorganism soil conditioner according to claim 8, it is characterised in that: Stover ferment agent in the fermentation step for bacillus subtilis or lactic acid bacteria or Bifidobacterium Bifidum or saccharomycete or actinomyces or Zytase or acetic acid bacteria.
CN201711049378.0A 2017-10-31 2017-10-31 A kind of preparation method suitable for harmful microorganism soil conditioner Pending CN107955616A (en)

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CN109593015A (en) * 2018-12-25 2019-04-09 湖南恒凯环保科技投资有限公司 Utilize the water paddy soil modifying agent and preparation method thereof of solid waste preparation
CN109680697A (en) * 2019-02-26 2019-04-26 洛阳智昊工程科技有限公司 Slope protection ecologic blanket for sandstone factory ecological recovery
CN110128221A (en) * 2019-06-27 2019-08-16 沈阳农业大学 A kind of straw biological charcoal base soil phosphorus reactive conditioners and preparation method thereof
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CN108580545A (en) * 2018-04-28 2018-09-28 辽宁大学 A method of cooperate with earthworm and Chinese pennisetum joint to repair and discard chromium slag place using biomass carbon
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CN110128221A (en) * 2019-06-27 2019-08-16 沈阳农业大学 A kind of straw biological charcoal base soil phosphorus reactive conditioners and preparation method thereof
US11124461B2 (en) 2019-07-04 2021-09-21 Incitec Pivot Limited Fertilizer
US11691929B2 (en) 2019-07-04 2023-07-04 Incitec Fertilizers Pty Limited Fertiliser

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