CN107753308A - A kind of oral care implement containing biological lysozyme - Google Patents
A kind of oral care implement containing biological lysozyme Download PDFInfo
- Publication number
- CN107753308A CN107753308A CN201710922542.8A CN201710922542A CN107753308A CN 107753308 A CN107753308 A CN 107753308A CN 201710922542 A CN201710922542 A CN 201710922542A CN 107753308 A CN107753308 A CN 107753308A
- Authority
- CN
- China
- Prior art keywords
- lysozyme
- oral care
- care implement
- biological
- additives
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
Abstract
The invention discloses a kind of oral care implement containing biological lysozyme, the oral care implement contains 0.1%~15%（wt）Biological lysozyme compositions of additives, the biological lysozyme compositions of additives is made up of each component of following percentage by weight：Biological lysozyme 10~40%, modified plant gum 0.01~2.0%, EDETATE SODIUM 0.01~0.3%, deionized water surplus.Oral care product provided by the invention uses a kind of biological lysozyme compositions of additives of special formulation, the use of said composition, the biological lysozyme in dental products can be made to be added to be influenceed from denaturation of the materials such as foaming agent, binding agent, sweetener, preservative on lysozyme, so as to prevent that biological lysozyme activity is not destroyed or reduces.Therefore, oral care implement provided by the invention can efficiently press down the harmful bacteria for killing and causing the oral problems such as canker sore, gingivitis, implication halitosis.
Description
Technical field
The present invention relates to shield product technical field is washed, in particular it relates to which a kind of oral care containing biological lysozyme is used
Product.
Background technology
The oral cavity of people is an intact ecocystem, is provided to the growth and breeding of various microorganisms and colonization in oral cavity
The environment and condition being particularly suitable for.In oral cavity the microorganism of field planting include the complicated bacterium of species, fungi, mycoplasma, protozoon and
Virus, these microorganisms constitute oral microecosystem in oral cavity different parts commensalism competition and antagonism, strong with host buccal
Health and disease close relation.Oral health behaviours imbalance factor is complicated, can be age, diet, health, health status
And use of antibiotic etc., it is also possible to the influence of the interphase interaction of microorganism, and ultimately result in microbial state treatment.Oral cavity
Disease causes Oral health behaviours to be lacked of proper care, otherwise Oral health behaviours imbalance can also produce mouth disease.Using effective gargle,
The measures such as the treatment of toothpaste and mouth disease can substantially reduce the quantity of pathogenic bacteria in oral cavity and improve or even eliminate oral cavity
Disease.But there is the problem of some can not be ignored in use in current market sales of a variety of dental products,
Such as fluoride toothpaste causes chemical bacteriostatic agent in fluorine poisoning, toothpaste to cause oral bacteria resistance.Therefore, one kind is safer has
The research and development of the dental products of effect turn into the focus in the field.
Lysozyme is a kind of natural antibacterial albumen, is widely present in water, animal juice, tear, saliva, breast
In juice, birds, beasts and eggs and part bacterium.Lysozyme not only has muramidase activity, can hydrolyze -acetylmuramic acid in peptide glycan and
β-Isosorbide-5-Nitrae glycosidic bond between N-Acetyl-D-glucosamine, but also there is antibacterial peptide activity, this structure with lysozyme, electric charge point
Cloth and surface hydrophobic are relevant.Egg white lysozyme accounts for the 3.4%~3.5% of egg white total protein, is that current research and application are most
Lysozyme.White, micro- sweet tea, characteristic odorless, soluble in water, in acid condition thermostabilization is presented in egg white lysozyme freeze-dried powder
Property is very strong.Lysozyme is widely used in the anti-corrosion of food mainly as preservative at present, and security is good.
In summary, lysozyme has a high potential applied to the developing target market of oral-cavity article.However, in conventional oral-cavity article
Essential foaming agent, binding agent, sweetener, chemical preservative etc. can cause bacteriolyze enzyme denaturation in various degree, so as to
Cause its active reduction or loss.Therefore, effectively protection lysozyme activity is that it is applied to the emphasis and difficult point of oral-cavity article
One of.
The content of the invention
The invention aims to overcome the deficiencies in the prior art, there is provided a kind of oral care containing biological lysozyme
Articles for use.
To achieve these goals, the present invention is achieved by the following technical programs：
A kind of oral care implement containing biological lysozyme, the biological bacteriolyze enzyme additive containing 0.1~15% (wt)
Composition, the biological lysozyme compositions of additives are made up of each component of following percentage by weight：Biological lysozyme 10~
40%, modified plant gum 0.01~2.0%, EDETATE SODIUM 0.01~0.3%, deionized water surplus.
Preferably, the biological lysozyme compositions of additives is made up of each component of following percentage by weight：Biology is molten
Bacterium enzyme 20~30%, modified plant gum 0.1~1.0%, EDETATE SODIUM 0.1~0.3%, deionized water surplus.
Preferably, dosage of the biological lysozyme compositions of additives in oral-cavity article is 1~10%.
Preferably, the oral care implement is toothpaste, tooth powder, mouthwash, mouth spray or oral cleaning gel.
Preferably, the toothpaste contains each component of following percetage by weight：Biological lysozyme compositions of additives 0.1~
15%, NMF 10~70%, rubbing agent 10~50%, thickener 0.01~3%, foaming agent 1~3%, sweetener 0.01~
1%, essence 0.1~2%.
It is highly preferred that the toothpaste contains each component of following percetage by weight：Biological lysozyme compositions of additives
3.75~15%, NMF 40~65%, rubbing agent 15~20%, thickener 0.7~1.2%, foaming agent 1.5~2.5%, sweet tea
Taste agent 0.25%, essence 1%.
Most preferably, the toothpaste contains each component of following percetage by weight：Biological lysozyme compositions of additives
6%, NMF 10~70%, rubbing agent 10~50%, thickener 0.01~3%, foaming agent 1~3%, sweetener 0.01~
1%, essence 0.1~2%；The biological lysozyme compositions of additives is made up of each component of following percentage by weight：Biology
Lysozyme 25%, carrageenan 0.5%, EDETATE SODIUM 0.2%, deionized water surplus.
Most preferably, the toothpaste contains each component of following percetage by weight：Biological lysozyme compositions of additives
6%, NMF 40~65%, rubbing agent 15~20%, thickener 0.7~1.2%, foaming agent 1.5~2.5%, sweetener
0.25%, essence 1%；The biological lysozyme compositions of additives is made up of each component of following percentage by weight：Biology is molten
Bacterium enzyme 25%, carrageenan 0.5%, EDETATE SODIUM 0.2%, deionized water surplus.
Most preferably, the toothpaste contains each component of following percetage by weight：Biological lysozyme compositions of additives
3.75%, NMF 10~70%, rubbing agent 10~50%, thickener 0.01~3%, foaming agent 1~3%, sweetener 0.01
~1%, essence 0.1~2%；The biological lysozyme compositions of additives is made up of each component of following percentage by weight：It is raw
Thing lysozyme 40%, guar gum 0.1%, carrageenan 0.7%, EDETATE SODIUM 0.3%, deionized water surplus.
Most preferably, the toothpaste contains each component of following percetage by weight：Biological lysozyme compositions of additives
3.75, NMF 40~65%, rubbing agent 15~20%, thickener 0.7~1.2%, foaming agent 1.5~2.5%, sweetener
0.25%, essence 1%；The biological lysozyme compositions of additives is made up of each component of following percentage by weight：Biology is molten
Bacterium enzyme 40%, guar gum 0.1%, carrageenan 0.7%, EDETATE SODIUM 0.3%, deionized water surplus.
Most preferably, the toothpaste is made up of each component of following percetage by weight：Biological lysozyme compositions of additives
3.75~15%, sorbierite 25%, glycerine 20%, rubbing agent 20%, sodium carboxymethylcellulose 0.8%, laruyl alcohol sodium sulfovinate
2%, sweetener 0.25%, essence 1%, deionized water surplus.
Most preferably, the toothpaste is made up of each component of following percetage by weight：Biological lysozyme compositions of additives
5%, sorbierite 60%, glycerine 5%, rubbing agent 15%, sodium carboxymethylcellulose 0.7%, laruyl alcohol sodium sulfovinate 1.5%, the moon
Osmanthus acylsarcosine sodium 1.0%, sodium phytate 1.0%, sweetener 0.25%, essence 1%, deionized water surplus.
Most preferably, the toothpaste is made up of each component of following percetage by weight：Biological lysozyme compositions of additives
3.75~15%, sorbierite 20%, glycerine 10%, PEG-4000 10%, rubbing agent 20%, sodium carboxymethylcellulose
0.8%, xanthans 0.3%, laruyl alcohol sodium sulfovinate 2%, sodium fluoride 0.32%, sweetener 0.25%, essence 1%, deionization
Water surplus.
Most preferably, the toothpaste is made up of each component of following percetage by weight：Biological lysozyme compositions of additives
5%, sorbierite 30%, glycerine 10%, polyethylene glycol -6005%, rubbing agent 15%, hydroxyapatite 5%, carboxymethyl cellulose
Sodium 0.7%, xanthans 0.5%, laruyl alcohol sodium sulfovinate 1.5%, potassium nitrate 3.0%, strontium chloride 0.3%, sweetener 0.25%,
Essence 1%, deionized water surplus.
Preferably, the mouthwash contains each component of following percetage by weight, biological lysozyme compositions of additives 0.1
~15%, NMF 1~30%, emulsifying agent 0.01~2%, sweetener 0.001~1%, essence 0.01~1%, coolant agent
0.001~0.2%.
It is highly preferred that the mouthwash contains each component of following percetage by weight, biological lysozyme compositions of additives
3.75~15%, NMF 19~25%, emulsifying agent 1%, sweetener 0.05~1%, essence 0.2%, coolant agent 0.05%.
Most preferably, the mouthwash contains each component of following percetage by weight, biological lysozyme compositions of additives
5%, NMF 1~30%, emulsifying agent 0.01~2%, sweetener 0.001~1%, essence 0.01~1%, coolant agent 0.001
~0.2%；The biological lysozyme compositions of additives is made up of each component of following percentage by weight：Biological lysozyme
20%, locust bean gum or sodium alginate 0.1%, EDETATE SODIUM 0.2%, deionized water surplus.
Most preferably, the mouthwash contains each component of following percetage by weight, biological lysozyme compositions of additives
5%, NMF 19~25%, emulsifying agent 1%, sweetener 0.05~1%, essence 0.2%, coolant agent 0.05%；The biology
Lysozyme compositions of additives is made up of each component of following percentage by weight：Biological lysozyme 20%, locust bean gum or marine alga
Sour sodium 0.1%, EDETATE SODIUM 0.2%, deionized water surplus.
Most preferably, the mouthwash is made up of each component of following percetage by weight：Biological bacteriolyze enzyme additive combination
Thing 5%, sorbierite 15%, PEG4005%, propane diols 5%, rilanit special 1%, xylitol 0.5%, sodium phosphate 0.05%,
Essence 0.2%, coolant agent 0.05%, deionized water surplus.
Most preferably, the mouthwash is made up of each component of following percetage by weight：Biological bacteriolyze enzyme additive combination
Thing 3.75~15%, sorbierite 5%, glycerine 10%, propane diols 5%, poloxamer188 1%, sodium fluoride 0.1%, saccharin sodium
0.05%, disodium hydrogen phosphate 0.02%, citric acid 0.01%, essence 0.2%, coolant agent 0.05%, deionized water surplus.
Most preferably, the mouthwash is made up of each component of following percetage by weight：Biological bacteriolyze enzyme additive combination
Thing 5%, sorbierite 8%, glycerine 6%, propane diols 5%, poloxamer188 1%, xylitol 1%, disodium hydrogen phosphate 0.02%,
Sodium dihydrogen phosphate 0.02%, essence 0.2%, coolant agent 0.05%, deionized water surplus.
Most preferably, the mouthwash is made up of each component of following percetage by weight：Biological bacteriolyze enzyme additive combination
Thing 3.75~15%, sorbierite 12%, glycerine 6%, PEG4003%, poloxamer188 1%, saccharin sodium 0.05%, phosphoric acid hydrogen
Disodium 0.03%, citric acid 0.01%, essence 0.2%, coolant agent 0.05%, deionized water surplus.
Preferably, the oral care implement sprays for mouth, each component of the mouth spray containing following percetage by weight, biological bacteriolyze
Enzyme additive composition 0.1~15%, NMF 1~20%, emulsifying agent 0.01~2%, sweetener 0.001~1%, essence
0.01~1%, coolant agent 0.001~0.3%.
It is highly preferred that the formula of the mouth spray is biological lysozyme compositions of additives 1.25~5%, and glycerine 15%, mountain
Pears alcohol 5%, rilanit special 1%, xylitol 0.5%, essence 0.3%, freshener 0.1%, deionized water surplus.
Preferably, the oral care implement is tooth powder, and tooth powder contains each component of following percetage by weight, biological bacteriolyze
Enzyme additive composition 0.1~15%, NMF 1~10%, rubbing agent 30~95%, thickener 0.01~1%, foaming agent 1
~3%, sweetener 0.01~5%, essence 0.1~2%, menthol 0.01~0.5%.
It is highly preferred that the formula of the tooth powder is biological lysozyme compositions of additives 5%, and sorbierite 10%, rubbing agent
70%, foaming agent 2.2%, sweetener 0.35%, essence 1.5%, deionized water surplus.
Preferably, the oral care implement is oral cleaning gel, and oral cleaning gel contains following percetage by weight
Each component, biological lysozyme compositions of additives 0.1~15%, NMF 1~70%, hydrated silica 1~20%, thickener
0.01~1%, foaming agent 0.01~2%, sweetener 0.01~5%, essence 0.1~1%.
It is highly preferred that the formula of the oral cleaning gel is biological lysozyme compositions of additives 10%, sorbierite
58%, glycerine 10%, hydrated silica 10%, carbomer 0.3%, 50% sodium hydroxide 0.2%, xylitol 1%, essence 1%, go
Ion water surplus.
Compared with prior art, the present invention has the advantages that：
Oral care product provided by the invention uses a kind of biological lysozyme compositions of additives of special formulation, should
The use of composition, it can make to be added to the biological lysozyme in dental products from foaming agent, binding agent, sweetener, anti-corrosion
Denaturation of the materials such as agent on lysozyme influences, so as to prevent that biological lysozyme activity is not destroyed or reduces.Therefore, the present invention carries
The oral care implement of confession can efficiently press down the large intestine bar for killing and causing the oral problems such as canker sore, gingivitis, implication halitosis
Bacterium and staphylococcus aureus etc..
Embodiment
The present invention is made with reference to specific embodiment and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is normal unless otherwise specified
Rule method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
Embodiment 1
A kind of compositions of additives containing biological lysozyme, it is formulated matches somebody with somebody for any one in the formula 1~14 in table 1
Side.
The preparation method of compositions of additives containing biological lysozyme is：First EDETATE SODIUM, modified plant gum are dissolved in
In deionized water, dissolving is dispersed with stirring, improved plant glue solution is made；Biological lysozyme is added into above-mentioned improved plant glue
In solution, dispersing and dissolving is slowly stirred, is produced.
Afterwards with reference to the assay method of lysozyme in GB/T25879-2010 egg whites, formula 1~14 in table 1 is tested
The lysozyme activity of composition.It is first that the compositions of additives containing biological lysozyme of formula 1~14 in table 1 is first before test
1 is pressed with deionized water：100 ratio wiring solution-formings, 500 times are diluted with deionized water afterwards, is then detected again, and synchronously
The contrast test lysozyme activity of corresponding content biology lysozyme.As a result following Tables 1 and 2.
Table 1 is the composition of formula 1~14
The lysozyme activity of the corresponding content biology lysozyme of table 2
| Composition | Formula 1 | Formula 2 | Formula 3 | Formula 4 | Formula 5 | Formula 6 |
| Biological lysozyme | 10 | 15 | 20 | 25 | 30 | 40 |
| Deionized water | 90 | 85 | 80 | 75 | 70 | 60 |
| It is total | 100% | 100% | 100% | 100% | 100% | 100% |
| Lysozyme activity | 6501 | 9912 | 12815 | 20900 | 24000 | 29789 |
From the result of Tables 1 and 2, compositions of additives does not have negatively influencing to lysozyme enzymatic activity.
Application examples 1
Compositions of additives containing biological lysozyme corresponding to formula 1~6 in the table 1 of embodiment 1 is applied in toothpaste,
The specific formula of toothpaste is shown in Table 3.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, the corresponding toothpaste of measure application examples 1~6
Lysozyme activity.Toothpaste is diluted with 10 times of deionized water, is dispersed with stirring uniformly about 30 minutes, centrifuging and taking supernatant.Will be upper
Clear liquid is detected after diluting 500 times with deionized water.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding toothpaste of measure application examples 1~6 is to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of streptococcus mutans.And synchronism detection control 1, control 1 is i.e. conventionally
Biological lysozyme is directly added into toothpaste, measurement result such as table 3.
Table 3
Note：Application examples 1~6 and the content of the biological lysozyme in control 1 are identicals in table 3.
Being applied to different ratio containing biological lysozyme compositions of additives can be seen that using result according to table 3
In toothpaste, the enzymatic activity and bacteriostasis rate of enzymatic activity and the independent biological lysozyme raw material of addition of bacteriostasis rate ratio in toothpaste improve,
And there is significant difference.The present invention is by biological lysozyme and modified plant gum, EDETATE SODIUM, deionized water according to specifically matching somebody with somebody
Than combining, biological lysozyme can be protected from the influence of other compositions in toothpaste, protect the activity of biological lysozyme.
In addition, the result of application examples can also be seen that lysozyme content is consistent in control application examples from table 3, addition is not
With the composition of proportioning, it is found that the composition degree that lysozyme activity changes in application examples of different ratio is inconsistent.Therefore,
It can be seen that in composition modified plant gum species and the content of EDETATE DISODIUM can influence composition be applied to it is molten in product
Bacterium enzymatic activity.This illustrates different types of modified plant gum and different amounts of EDETATE SODIUM to biological lysozyme activity degree of protection
It is different.Identical using EDETATE SODIUM addition such as application examples 1 and 3, the modified plant gum content of application examples 1 is higher than application examples 3
The content of middle modified adhesive, and the antalzyme activity of application examples 3 changes degree and is slightly above the antalzyme activity of application examples 1 change degree,
This explanation compounding use sodium alginate and guar gum modified plant gum are better than a kind of hydroxyethyl cellulose glue effect is used alone.It is right
It is identical with modified plant gum species using EDETATE SODIUM addition than application examples 2 and 5, and the antalzyme activity of application examples 5 changes
Degree changes degree higher than the antalzyme activity of application examples 2, because the locust bean gum addition of application examples 5 is higher than application examples 2.This
Illustrate that the increase of modified plant gum amount has more preferable protection to lysozyme activity.Application examples 3 and 4 is contrasted, application examples 3 is planted using modified
Thing glue amount is higher than application examples 4, and the lysozyme activity of application examples 3 is far below application examples 4, and this explanation EDETATE SODIUM addition is to bacteriolyze
Enzymatic activity confining force influence degree is big, and lysozyme activity is raised and strengthened with EDETATE SODIUM addition.Contrast application examples 1 and 4
Also this point can be illustrated.
Application examples 2
Compositions of additives containing biological lysozyme corresponding to formula 7~14 in the table 1 of embodiment 1 is applied to toothpaste
In, the specific formula of toothpaste is shown in Table 4.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, the corresponding toothpaste of measure application examples 1~8
Lysozyme activity.Toothpaste is diluted with 10 times of deionized water, is dispersed with stirring uniformly about 30 minutes, centrifuging and taking supernatant.Will be upper
Clear liquid is detected after diluting 500 times with deionized water.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding toothpaste of measure application examples 1~8 is to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of streptococcus mutans.And synchronism detection control 1, control 1 is i.e. conventionally
Biological lysozyme is directly added into toothpaste, measurement result such as table 4.
Table 4
Note：Application examples 1~8 and the content of the biological lysozyme in control 1 are identicals in table 4.
Application examples 3
Compositions of additives containing biological lysozyme corresponding to formula 1~6 in the table 1 of embodiment 1 is applied in toothpaste,
The specific formula of toothpaste is shown in Table 5.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, the corresponding toothpaste of measure application examples 1~6
Lysozyme activity.Toothpaste is diluted with 10 times of deionized water, is dispersed with stirring uniformly about 30 minutes, centrifuging and taking supernatant.Will be upper
Clear liquid is detected after diluting 500 times with deionized water.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding toothpaste of measure application examples 1~6 is to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of streptococcus mutans.And synchronism detection control 1, control 1 is i.e. conventionally
Biological lysozyme is directly added into toothpaste, measurement result such as table 5.
Table 5
Note：Application examples 1~6 and the content of the biological lysozyme in control 1 are identicals in table 5.
Application examples 4
Compositions of additives containing biological lysozyme corresponding to formula 7~14 in the table 1 of embodiment 1 is applied to toothpaste
In, the specific formula of toothpaste is shown in Table 6.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, the corresponding toothpaste of measure application examples 1~8
Lysozyme activity.Toothpaste is diluted with 10 times of deionized water, is dispersed with stirring uniformly about 30 minutes, centrifuging and taking supernatant.Will be upper
Clear liquid is detected after diluting 500 times with deionized water.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding toothpaste of measure application examples 1~8 is to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of streptococcus mutans.And synchronism detection control 1, control 1 is i.e. conventionally
Biological lysozyme is directly added into toothpaste, measurement result such as table 6.
Table 6
Note：Application examples 1~8 and the content of the biological lysozyme in control 1 are identicals in table 6.
The present invention passes through the biological lysozyme compositions of additives of addition special formulation in the toothpaste to different formulations, toothpaste
The activity of middle biological lysozyme does not reduce, and this illustrates that biological lysozyme compositions of additives can be added to any formula
In toothpaste, therefore, although the present invention is only illustrated with the toothpaste of four kinds of different formulations, this does not represent biological bacteriolyze
Enzyme additive composition can be only used in the toothpaste of above-mentioned four kinds of different formulations.
Application examples 5
Will the compositions of additives containing biological lysozyme be added to gargling corresponding to formula 7~14 in the table 1 of embodiment 1
In water.The formula of each mouthwash is shown in Table the application examples 1~8 in 7.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, 1~8 corresponding mouthwash of application examples is determined
Lysozyme activity.Mouthwash is diluted with 10 times of deionized water, centrifuging and taking supernatant dilutes 500 times with deionized water afterwards
After detected.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, determines the mouthwash of application examples 1~8 to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of Streptococcus mutans.And control 1 is synchronously set, control 1 is conventionally
Biological lysozyme is directly added into mouthwash, measurement result such as table 7.
Table 7
Note：Application examples 1~8 and the content of the biological lysozyme of control 1 are identicals in table 7.
Being applied to different ratio containing biological lysozyme compositions of additives can be seen that using result according to table 7
In mouthwash, the enzymatic activity and bacteriostasis rate of enzymatic activity and the independent biological lysozyme raw material of addition of bacteriostasis rate ratio in mouthwash carry
Height, and there is significant difference.The present invention is by biological lysozyme with modified plant gum, EDETATE SODIUM, deionized water according to specific
Proportioning combine, biological lysozyme can be protected from the influence of other compositions in mouthwash, protect biological lysozyme
Activity.It should be noted that when, the amount addition of modified plant gum is excessive, and mouthwash mouthfeel can be had a negative impact.
In addition, the result of application examples can also be seen that lysozyme content is consistent in control application examples from table 7, addition is not
With the composition of proportioning, it is found that the composition degree that lysozyme activity changes in application examples of different ratio is inconsistent.Therefore,
It can be seen that in composition modified plant gum species and the content of EDETATE DISODIUM can influence composition be applied to it is molten in product
Bacterium enzymatic activity.This illustrates different types of modified plant gum and different amounts of EDETATE SODIUM to biological lysozyme activity degree of protection
It is different.For example, although the Comparative result of application examples 1 and 4 can be seen that the hydroxyethyl cellulose that application examples 1 uses from table 7
Amount be greater than the amount of the sodium alginate that application examples 4 uses, but from the point of view of antalzyme activity changes situation, application examples 4 it is molten
Bacterium enzyme activity improves degree and is better than application examples 1, therefore, it can be seen that protective effect of the sodium alginate to biological lysozyme is better than
Hydroxyethyl cellulose.Application examples 1 and application examples 2 use same amount of modified plant gum, but the antalzyme activity of application examples 1 carries
High level is better than application examples 2, and reason is that the modified plant gum that application examples 1 uses is hydroxyethyl cellulose, what application examples 2 used
Modified plant gum is carrageenan.Although the carrageenan that the amount and application examples 2 of the hydroxyethyl cellulose that application examples 1 uses use
Amount be more than the amount of locust bean gum that application examples 3 uses, but from the point of view of antalzyme activity changes situation, the bacteriolyze of application examples 3
Enzyme activity improves degree and is significantly better than application examples 1 and 2, and this protective effect of explanation locust bean gum to biological lysozyme is better than hydroxyl
Ethyl cellulose, carrageenan.
Application examples 6
Will the compositions of additives containing biological lysozyme be added to gargling corresponding to formula 1~6 in the table 1 of embodiment 1
In water.The formula of each mouthwash is shown in Table the application examples 1~6 in 8.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, 1~6 corresponding mouthwash of application examples is determined
Lysozyme activity.Mouthwash is diluted with 10 times of deionized water, centrifuging and taking supernatant dilutes 500 times with deionized water afterwards
After detected.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, determines the mouthwash of application examples 1~6 to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of Streptococcus mutans.And control 1 is synchronously set, control 1 is conventionally
Biological lysozyme is directly added into mouthwash, measurement result such as table 8.
Table 8
Note：Application examples 1~6 and the content of the biological lysozyme in control 1 are identicals in table 8.
Application examples 7
Will the compositions of additives containing biological lysozyme be added to gargling corresponding to formula 7~14 in the table 1 of embodiment 1
In water.The formula of each mouthwash is shown in Table the application examples 1~8 in 9.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, 1~8 corresponding mouthwash of application examples is determined
Lysozyme activity.Mouthwash is diluted with 10 times of deionized water, centrifuging and taking supernatant dilutes 500 times with deionized water afterwards
After detected.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, determines the mouthwash of application examples 1~8 to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of Streptococcus mutans.And control 1 is synchronously set, control 1 is conventionally
Biological lysozyme is directly added into mouthwash, measurement result such as table 9.
Table 9
Note：Application examples 1~8 and the content of the biological lysozyme of control 1 are identicals in table 9.
Application examples 8
Will the compositions of additives containing biological lysozyme be added to gargling corresponding to formula 1~6 in the table 1 of embodiment 1
In water.The formula of each mouthwash is shown in Table the application examples 1~6 in 10.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, 1~6 corresponding mouthwash of application examples is determined
Lysozyme activity.Mouthwash is diluted with 10 times of deionized water, centrifuging and taking supernatant dilutes 500 times with deionized water afterwards
After detected.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, determines the mouthwash of application examples 1~6 to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of Streptococcus mutans.And control 1 is synchronously set, control 1 is conventionally
Biological lysozyme is directly added into mouthwash, measurement result such as table 10.
Table 10
Note：Application examples 1~6 and the content of the biological lysozyme in control 1 are identicals in table 10.
The present invention is gargled by the biological lysozyme compositions of additives of addition special formulation in the mouthwash to different formulations
The activity of mouth organism in water lysozyme does not reduce, and this illustrates that biological lysozyme compositions of additives can be added to any match somebody with somebody
In the mouthwash of side, therefore, although the present invention is only illustrated with the mouthwash of four kinds of different formulations, this is not represented
Biological lysozyme compositions of additives can be only used in the mouthwash of above-mentioned four kinds of different formulations.
Application examples 9
Compositions of additives containing biological lysozyme corresponding to formula 1~6 in the table 1 of embodiment 1 is added to mouth and sprayed
In.The formula of each mouth spray is shown in Table the application examples 1~6 in 11.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, the corresponding mouth spray of measure application examples 1~6
Lysozyme activity.Mouth spray is diluted with 10 times of deionized water, centrifuging and taking supernatant is examined after diluting 500 times with deionized water
Survey.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding mouth spray of measure application examples 1~6 is to Escherichia coli, golden yellow grape
Coccus, porphyromonas gingivalis, the inhibitory action of Streptococcus mutans.And control 1 is synchronously set, control 1 is conventionally
Biological lysozyme is directly added into mouth spray, measurement result such as table 11.
Table 11
Note：Application examples 1~6 and the content of the biological lysozyme in control 1 are identicals in table 11.
Be can be seen that according to table 11 using result should containing biological lysozyme compositions of additives by different composition and ratios
Use in mouth spray, the enzymatic activity and bacteriostasis rate of enzymatic activity and the independent biological lysozyme raw material of addition of bacteriostasis rate ratio in mouth spray are equal
Height, and there is significant difference.The present invention is by biological lysozyme with modified plant gum, EDETATE SODIUM, deionized water according to specific
Proportioning combine, can protect biological lysozyme from mouth spray in other compositions influence, protect biological lysozyme
Activity.
Combined in addition, can be seen that according to the Comparative result of table 9 and table 11 by same amount of containing biological bacteriolyze enzyme additive
Thing is respectively added in toothpaste and mouth spray, and the activity change degree of biological lysozyme is different.Lysozyme activity improves in toothpaste
Degree is more than the active raising degree in mouth spray.In toothpaste, compositions of additives embodies higher, guarantor to lysozyme protecting effect
It is more preferable to protect effect.On the other hand also illustrate individually lysozyme powder is added in toothpaste, other compositions are to lysozyme in toothpaste
Activity influence is very big.So as to can more embody what biological lysozyme compositions of additives of the present invention rose to lysozyme
Protective effect.
Application examples 10
Compositions of additives containing biological lysozyme corresponding to formula 7~14 in the table 1 of embodiment 1 is added to tooth powder
In.The formula of each tooth powder is shown in Table the application examples 1~8 in 12.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, the corresponding tooth powder of measure application examples 1~8
Lysozyme activity.And control 1 is synchronously set, control 1 determines for conventionally biological lysozyme is directly added into tooth powder
As a result such as table 12.Tooth powder is diluted with 10 times of deionized water, is dispersed with stirring about 30 minutes, centrifuging and taking supernatant.By supernatant
Detected after diluting 500 times with deionized water.
Table 12
Note：Application examples 1~8 and the content of the biological lysozyme in control 1 are identicals in table 12.
According to the application result of table 12, different composition and ratios are applied to tooth powder containing biological lysozyme compositions of additives
In, activity ratio individually adds enzymatic activity height of the biological lysozyme raw material in tooth powder, and has significant difference.The present invention will
Biological lysozyme is combined with modified plant gum, EDETATE SODIUM, deionized water according to specific proportioning, can protect biology
Lysozyme protects the activity of biological lysozyme from the influence of other compositions in tooth powder.
It is can be seen that according to the Comparative result of table 12 and table 10 by same amount of containing biological lysozyme compositions of additives point
It is not added in mouthwash and tooth powder, the activity change degree of biological lysozyme is different, will contain biological bacteriolyze enzyme additive combination
Thing is added in mouthwash, and the vigor of biological lysozyme, which improves degree and is noticeably greater than, to be added in tooth powder, the possibility of this species diversity
Reason lysozyme powder is added in tooth powder, and due to the mixing between solid powder, the intermolecular forces and influence power of molecule compare liquid
Weak, influence of the other compositions to lysozyme reacts fierce not in liquid condition.Thus lysozyme powder is added in tooth powder
Activity reduces little.Lysozyme activity change in mouthwash degree ratio change in tooth powder it is high.
Application examples 11
Compositions of additives containing biological lysozyme corresponding to formula 7~14 in the table 1 of embodiment 1 is added to oral cavity
Clean in gel product.The formula of each oral cleaning gel product is shown in Table the application examples 1~8 in 13.
With reference to the assay method of lysozyme in GB/T25879-2010 egg whites, the corresponding oral cavity of measure application examples 1~8 is clear
The lysozyme activity of clean gel product.Gel is diluted with 10 times of deionized water, is dispersed with stirring about 30 minutes, centrifuging and taking supernatant
Liquid.Detected after supernatant is diluted into 500 times with deionized water.
It is molten with reference to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB15979-2002C4
Going out property resists (suppression) bacterium product bacteriostasis property test method, and the measure corresponding oral cleaning gel product of application examples 1~8 is to large intestine bar
Bacterium, staphylococcus aureus, porphyromonas gingivalis, the inhibitory action of Streptococcus mutans.And control 1 is synchronously set, control 1 is
Conventionally biological lysozyme is directly added into oral cleaning gel product, measurement result such as table 13.
Table 13
Note：Application examples 1~8 and the content of the biological lysozyme in control 1 are identicals in table 13.
According to the application result of table 13, different composition and ratios are applied to oral cavity containing biological lysozyme compositions of additives
Clean in gel, activity ratio individually adds enzymatic activity height of the biological lysozyme raw material in tooth powder, and has significant difference.
The present invention combines biological lysozyme and modified plant gum, EDETATE SODIUM, deionized water according to specifically matching, can be with
Protect biological lysozyme from the influence of other compositions in oral cleaning gel, protect the activity of biological lysozyme.
In addition, activity ratio's application examples 5 of application examples 6,7,8 is slightly higher in table 13, may be with guar gum dosage in composition
Increase relevant.Guar gum dosage increase in composition, formation tridimensional network is closer, and coating function is more preferable, and lysozyme exists
Activity can be protected more preferably in product.Activity influence effect of the variety classes modified plant gum to lysozyme is more or less the same.
It is effective in described protection domain.
It can be seen that according to the Comparative result of table 13, table 12 and table 10 and contain biological bacteriolyze enzyme additive group by same amount of
Compound is respectively added in mouthwash, tooth powder, oral cleaning gel, and the activity change degree of biological lysozyme is different.
Claims (9)
1. a kind of oral care implement containing biological lysozyme, it is characterised in that contain 0.1~15%（wt）Biological bacteriolyze
Enzyme additive composition, the biological lysozyme compositions of additives are made up of each component of following percentage by weight：Biology is molten
Bacterium enzyme 10~40%, modified plant gum 0.01~2.0%, EDETATE SODIUM 0.01~0.3%, deionized water surplus.
2. oral care implement according to claim 1, it is characterised in that the biological lysozyme compositions of additives by
The each component composition of following percentage by weight：Biological lysozyme 20~30%, modified plant gum 0.1~1.0%, EDETATE SODIUM 0.1
~0.3%, deionized water surplus.
3. oral care implement according to claim 1, it is characterised in that the biological lysozyme compositions of additives exists
Dosage in oral-cavity article is 1~10%.
4. oral care implement according to claim 1, it is characterised in that the oral care implement be toothpaste, tooth powder,
Mouthwash, mouth spray or oral cleaning gel.
5. oral care implement according to claim 4, it is characterised in that the oral care implement is toothpaste, toothpaste
Each component containing following percetage by weight：Biological lysozyme compositions of additives 0.1~15%, NMF 10~70%, friction
Agent 10~50%, thickener 0.01~3%, foaming agent 1~3%, sweetener 0.01~1%, essence 0.1~2%.
6. oral care implement according to claim 4, it is characterised in that the oral care implement is mouthwash, is gargled
Saliva contains each component of following percetage by weight, biological lysozyme compositions of additives 0.1~15%, NMF 1~30%, breast
Agent 0.01~2%, sweetener 0.001~1%, essence 0.01~1%, coolant agent 0.001~0.2%.
7. oral care implement according to claim 4, it is characterised in that the oral care implement sprays for mouth, mouth spray
Each component containing following percetage by weight, biological lysozyme compositions of additives 0.1~15%, NMF 1~20%, emulsifying agent
0.01~2%, sweetener 0.001~1%, essence 0.01~1%, coolant agent 0.001~0.3%.
8. oral care implement according to claim 4, it is characterised in that the oral care implement is tooth powder, tooth powder
Each component containing following percetage by weight, biological lysozyme compositions of additives 0.1~15%, NMF 1~10%, rubbing agent
30~95%, thickener 0.01~1%, foaming agent 1~3%, sweetener 0.01~5%, essence 0.1~2%, menthol 0.01~
0.5%。
9. oral care implement according to claim 4, it is characterised in that the oral care implement is oral cleaning Gel
Curry, oral cleaning gel contain each component of following percetage by weight, biological lysozyme compositions of additives 0.1~15%, moisturizing
Agent 1~70%, hydrated silica 1~20%, thickener 0.01~1%, foaming agent 0.01~2%, sweetener 0.01~5%, essence 0.1
~1%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710922542.8A CN107753308B (en) | 2017-09-30 | 2017-09-30 | A kind of oral care implement containing biological lysozyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710922542.8A CN107753308B (en) | 2017-09-30 | 2017-09-30 | A kind of oral care implement containing biological lysozyme |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107753308A true CN107753308A (en) | 2018-03-06 |
| CN107753308B CN107753308B (en) | 2019-03-22 |
Family
ID=61266323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710922542.8A Active CN107753308B (en) | 2017-09-30 | 2017-09-30 | A kind of oral care implement containing biological lysozyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107753308B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108498401A (en) * | 2018-05-25 | 2018-09-07 | 钱兴 | A kind of preparation method of mouthwash |
| WO2020029352A1 (en) * | 2018-08-10 | 2020-02-13 | 阚向阳 | Processed cheese containing lysozyme and preparation method thereof |
| CN111053718A (en) * | 2019-12-31 | 2020-04-24 | 合肥赛为智慧医疗有限公司 | Mouthwash with function of resisting helicobacter pylori in oral cavity |
| CN111840126A (en) * | 2020-07-16 | 2020-10-30 | 上海中华药业有限公司 | Oral care agent with antibacterial effect and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434315A (en) * | 2016-01-08 | 2016-03-30 | 青岛康尔生物工程有限公司 | Gum-protecting whitening toothpaste and preparation method thereof |
| CN106265423A (en) * | 2016-08-23 | 2017-01-04 | 广州立白企业集团有限公司 | Having relieves inflammation or internal heat disappear pain effect oral care composition |
-
2017
- 2017-09-30 CN CN201710922542.8A patent/CN107753308B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434315A (en) * | 2016-01-08 | 2016-03-30 | 青岛康尔生物工程有限公司 | Gum-protecting whitening toothpaste and preparation method thereof |
| CN106265423A (en) * | 2016-08-23 | 2017-01-04 | 广州立白企业集团有限公司 | Having relieves inflammation or internal heat disappear pain effect oral care composition |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108498401A (en) * | 2018-05-25 | 2018-09-07 | 钱兴 | A kind of preparation method of mouthwash |
| WO2020029352A1 (en) * | 2018-08-10 | 2020-02-13 | 阚向阳 | Processed cheese containing lysozyme and preparation method thereof |
| CN111053718A (en) * | 2019-12-31 | 2020-04-24 | 合肥赛为智慧医疗有限公司 | Mouthwash with function of resisting helicobacter pylori in oral cavity |
| CN111840126A (en) * | 2020-07-16 | 2020-10-30 | 上海中华药业有限公司 | Oral care agent with antibacterial effect and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107753308B (en) | 2019-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107753308B (en) | A kind of oral care implement containing biological lysozyme | |
| JP5508005B2 (en) | Oral compositions and their manufacture and use | |
| CN106619193B (en) | A kind of antibacterial whitening toothpaste and preparation method thereof | |
| CN105326656A (en) | Oral antibacterial composition containing nisin and polylysine and preparation method thereof | |
| AU2003228954B8 (en) | Antibacterial dentifrice exhibiting enhanced antiplaque and breath freshening properties | |
| CN105902400B (en) | A kind of particle and its preparation method and application of wrapping biological enzyme | |
| CN110292045B (en) | Preparation method and application of antimicrobial composition containing epsilon-polylysine | |
| CN104936576A (en) | Oral care composition comprising an Amadori compound | |
| CN109908025A (en) | Application of the glycine betaine in protection lysozyme activity | |
| JP7003186B2 (en) | Composition containing meso-2,3-butanediol | |
| KR102272901B1 (en) | Composition for external application to the skin containing meso-2,3-butanediol as a preservative | |
| CN108553336A (en) | One kind is gargled pearl and its preparation method and application | |
| CN107693392B (en) | Toothpaste containing enzyme preparation and preparation method thereof | |
| CN111346124A (en) | Formula and preparation method of skin disinfection cleaning solution | |
| CN107582419B (en) | A kind of mouthwash containing biological lysozyme | |
| EP4164383A1 (en) | Novel use | |
| CN108652996A (en) | A kind of oral care health promoting liquid containing lysozyme | |
| CN107569400B (en) | A kind of toothpaste containing biological lysozyme | |
| KR20080060924A (en) | Low-irritative powder composition for washing | |
| CN100335028C (en) | Oral composition providing enhanced oral hygiene properties | |
| JPH09249541A (en) | Dentifrice | |
| CN112587421A (en) | Washing-free hand disinfection gel containing plant extracts and preparation method and application thereof | |
| CN107737024B (en) | A kind of compositions of additives containing biological lysozyme | |
| JP5140426B2 (en) | A disinfectant selective for caries-causing bacteria, a method of disinfecting caries-causing bacteria | |
| CN113813197B (en) | Composition for improving antibacterial activity and application of composition in oral product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180823 Address after: 511400 Room 601, development building, Panyu District energy conservation technology park, Guangzhou, Guangdong. Applicant after: Guangzhou Kang Yun Biotechnology Co., Ltd. Address before: 510000 No. five mountain road, Tianhe District, Guangzhou, Guangdong 381 Applicant before: Zhang Lei |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder |
Address after: Unit 306, Building 5 (2), Spiral Fourth Road, Guangzhou International Biological Island, Huangpu District, Guangzhou, Guangdong 510000 Patentee after: Guangzhou Kang Yun Biotechnology Co., Ltd. Address before: 511400 Room 601, development building, Panyu District energy conservation technology park, Guangzhou, Guangdong. Patentee before: Guangzhou Kang Yun Biotechnology Co., Ltd. |
|
| CP02 | Change in the address of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Unit 306, Building 5 (2), Spiral Fourth Road, Guangzhou International Biological Island, Huangpu District, Guangzhou, Guangdong 510000 Patentee after: Haoyikang Biotechnology (Guangzhou) Co., Ltd Address before: Unit 306, Building 5 (2), Spiral Fourth Road, Guangzhou International Biological Island, Huangpu District, Guangzhou, Guangdong 510000 Patentee before: GUANGZHOU KANGYUN BIOLOGICAL TECHNOLOGY Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |