CN107569400B - A kind of toothpaste containing biological lysozyme - Google Patents
A kind of toothpaste containing biological lysozyme Download PDFInfo
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- CN107569400B CN107569400B CN201710940410.8A CN201710940410A CN107569400B CN 107569400 B CN107569400 B CN 107569400B CN 201710940410 A CN201710940410 A CN 201710940410A CN 107569400 B CN107569400 B CN 107569400B
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- toothpaste
- lysozyme
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- 102000016943 Muramidase Human genes 0.000 title claims abstract description 112
- 108010014251 Muramidase Proteins 0.000 title claims abstract description 112
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 title claims abstract description 112
- 235000010335 lysozyme Nutrition 0.000 title claims abstract description 112
- 229960000274 lysozyme Drugs 0.000 title claims abstract description 111
- 239000004325 lysozyme Substances 0.000 title claims abstract description 111
- 239000000606 toothpaste Substances 0.000 title claims abstract description 80
- 229940034610 toothpaste Drugs 0.000 title claims abstract description 80
- 239000000203 mixture Substances 0.000 claims abstract description 61
- 239000000654 additive Substances 0.000 claims abstract description 51
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000008367 deionised water Substances 0.000 claims abstract description 31
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 31
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims abstract description 18
- 235000003599 food sweetener Nutrition 0.000 claims abstract description 17
- 239000003765 sweetening agent Substances 0.000 claims abstract description 17
- 239000004088 foaming agent Substances 0.000 claims abstract description 11
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 25
- 239000003795 chemical substances by application Substances 0.000 claims description 17
- 241000196324 Embryophyta Species 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000004909 Moisturizer Substances 0.000 claims description 8
- 230000001333 moisturizer Effects 0.000 claims description 8
- RRLOOYQHUHGIRJ-UHFFFAOYSA-M sodium;ethyl sulfate Chemical compound [Na+].CCOS([O-])(=O)=O RRLOOYQHUHGIRJ-UHFFFAOYSA-M 0.000 claims description 8
- 239000002562 thickening agent Substances 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 7
- 230000000996 additive effect Effects 0.000 claims description 7
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 7
- 235000010418 carrageenan Nutrition 0.000 claims description 7
- 239000000679 carrageenan Substances 0.000 claims description 7
- 229920001525 carrageenan Polymers 0.000 claims description 7
- 229940113118 carrageenan Drugs 0.000 claims description 7
- 229940088598 enzyme Drugs 0.000 claims description 7
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 claims description 7
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 6
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 6
- 229920002907 Guar gum Polymers 0.000 claims description 5
- 235000010417 guar gum Nutrition 0.000 claims description 5
- 239000000665 guar gum Substances 0.000 claims description 5
- 229960002154 guar gum Drugs 0.000 claims description 5
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 4
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 claims description 4
- 235000010493 xanthan gum Nutrition 0.000 claims description 4
- 239000000230 xanthan gum Substances 0.000 claims description 4
- 229920001285 xanthan gum Polymers 0.000 claims description 4
- 229940082509 xanthan gum Drugs 0.000 claims description 4
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 2
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims description 2
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 229920000161 Locust bean gum Polymers 0.000 claims description 2
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 claims description 2
- FENRSEGZMITUEF-ATTCVCFYSA-E [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] FENRSEGZMITUEF-ATTCVCFYSA-E 0.000 claims description 2
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 claims description 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 2
- 235000010420 locust bean gum Nutrition 0.000 claims description 2
- 239000000711 locust bean gum Substances 0.000 claims description 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 2
- 229940068918 polyethylene glycol 400 Drugs 0.000 claims description 2
- 235000010333 potassium nitrate Nutrition 0.000 claims description 2
- 239000004323 potassium nitrate Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 235000010413 sodium alginate Nutrition 0.000 claims description 2
- 239000000661 sodium alginate Substances 0.000 claims description 2
- 229940005550 sodium alginate Drugs 0.000 claims description 2
- 235000013024 sodium fluoride Nutrition 0.000 claims description 2
- 239000011775 sodium fluoride Substances 0.000 claims description 2
- 229940083982 sodium phytate Drugs 0.000 claims description 2
- 229910001631 strontium chloride Inorganic materials 0.000 claims description 2
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 claims description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims 1
- 229940040145 liniment Drugs 0.000 claims 1
- 239000000865 liniment Substances 0.000 claims 1
- 229940057847 polyethylene glycol 600 Drugs 0.000 claims 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 claims 1
- 235000019605 sweet taste sensations Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 25
- 241000894006 Bacteria Species 0.000 abstract description 16
- 238000009472 formulation Methods 0.000 abstract description 6
- 230000036541 health Effects 0.000 abstract description 4
- 239000003755 preservative agent Substances 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 206010006326 Breath odour Diseases 0.000 abstract description 3
- 208000032139 Halitosis Diseases 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 3
- 239000011230 binding agent Substances 0.000 abstract description 3
- 238000004925 denaturation Methods 0.000 abstract description 3
- 230000036425 denaturation Effects 0.000 abstract description 3
- 208000007565 gingivitis Diseases 0.000 abstract description 3
- 208000020670 canker sore Diseases 0.000 abstract description 2
- 230000000116 mitigating effect Effects 0.000 abstract 2
- 230000002272 anti-calculus Effects 0.000 abstract 1
- 230000000740 bleeding effect Effects 0.000 abstract 1
- 230000001151 other effect Effects 0.000 abstract 1
- 201000001245 periodontitis Diseases 0.000 abstract 1
- 210000004261 periodontium Anatomy 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 210000000214 mouth Anatomy 0.000 description 13
- 238000005259 measurement Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 8
- 102000002322 Egg Proteins Human genes 0.000 description 8
- 108010000912 Egg Proteins Proteins 0.000 description 8
- 235000014103 egg white Nutrition 0.000 description 8
- 210000000969 egg white Anatomy 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- 230000001629 suppression Effects 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 238000010998 test method Methods 0.000 description 5
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 4
- 241001478240 Coccus Species 0.000 description 4
- 241000605862 Porphyromonas gingivalis Species 0.000 description 4
- 241000194019 Streptococcus mutans Species 0.000 description 4
- 235000009754 Vitis X bourquina Nutrition 0.000 description 4
- 235000012333 Vitis X labruscana Nutrition 0.000 description 4
- 240000006365 Vitis vinifera Species 0.000 description 4
- 235000014787 Vitis vinifera Nutrition 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000007865 diluting Methods 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 230000005548 health behavior Effects 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- 235000019082 Osmanthus Nutrition 0.000 description 1
- 241000333181 Osmanthus Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229920003123 carboxymethyl cellulose sodium Polymers 0.000 description 1
- 229940063834 carboxymethylcellulose sodium Drugs 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000013368 commensalism Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000012969 defense response to bacterium Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940124274 edetate disodium Drugs 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000009740 moulding (composite fabrication) Methods 0.000 description 1
- 208000030194 mouth disease Diseases 0.000 description 1
- 229940051921 muramidase Drugs 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
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- Cosmetics (AREA)
Abstract
The invention discloses a kind of toothpaste containing biological lysozyme, the toothpaste contains 0.1%~15%（wt）Biological lysozyme compositions of additives, it is described biology lysozyme compositions of additives be grouped as by each group of following weight percent：Biological lysozyme 10~40%, modified plant gum 0.01~2.0%, EDETATE SODIUM 0.01~0.3%, deionized water surplus.Toothpaste provided by the invention uses a kind of biological lysozyme compositions of additives of special formulation, the use of the composition, the biological lysozyme being added in toothpaste can be made to influence from substances such as foaming agent, binder, sweetener, preservatives on the denaturation of lysozyme, so that biological lysozyme activity be prevented not to be destroyed or reduce.Toothpaste provided by the invention can effectively press down the harmful bacteria for killing and causing the oral problems such as canker sore, gingivitis, implication halitosis, a variety of oral problems can be improved, mitigate gingivitis bleeding gums, prevention and mitigation periodontitis, promote the health of periodontium, there is antibacterial, anti-calculus dentalis, preventing decayed tooth, mitigation halitosis and other effects.
Description
Technical field
The present invention relates to wash shield product technical field, and in particular, to a kind of toothpaste containing biological lysozyme.
Background technique
The oral cavity of people is an intact ecocystem, is provided to the growth and breeding and colonization of microorganisms various in oral cavity
The environment and condition being particularly suitable for.In oral cavity the microorganism of field planting include the bacterium of type complexity, fungi, mycoplasma, protozoon and
Virus, these microorganisms constitute oral microecosystem in oral cavity different parts commensalism competition and antagonism, strong with host buccal
Health and disease close relation.Oral health behaviours imbalance factor be it is complicated, can be age, diet, health, health status
And use of antibiotic etc., it is also possible to the influence to interact between microorganism, and eventually lead to microbial state treatment.Oral cavity
Disease causes Oral health behaviours to be lacked of proper care, otherwise Oral health behaviours imbalance also can produce mouth disease.External antibacterial material can press down
The undue growth of bacterium processed is but difficult rightly to maintain the microecological balance in oral cavity.Unsuitable medicament selection can not only
Inhibit or kill oral cavity pathogen, while also killing oral cavity and residing commensal bacteria, thus is likely to form influence Oral health behaviours
The stimulus of the long-term chronic of environment, it is also possible to make the conditioned pathogen in oral cavity generate drug resistance, and reduce oral cavity
Self-healing ability of each tissue to damage.
Toothpaste is that common cleaning supplies in daily life by single clean type toothpaste develop into great variety of goods, function
Can be various, the Mobyneb toothpaste of a brands up to a hundred meets the needs of the different levels level of consumption.Toothpaste quality quality, directly
Connect the health for affecting people.There is some cannot be neglected in use in current market sales of toothpaste
Problem, such as fluoride toothpaste cause that fluorine poisoning, chemical bacteriostatic agent causes oral bacteria drug resistance in toothpaste.Therefore, Yi Zhonggeng
Safely and effectively the research and development of dental products become the hot spot in the field.
Lysozyme is a kind of natural antibacterial albumen, is widely present in water, animal juice, tear, saliva, cream
In juice, birds, beasts and eggs and part bacterium.Lysozyme not only has muramidase activity, can hydrolyze -acetylmuramic acid in peptide glycan and
β-Isosorbide-5-Nitrae glycosidic bond between N-Acetyl-D-glucosamine, but also there is antibacterial peptide activity, this divides with the structure of lysozyme, charge
Cloth and surface hydrophobic are related.Egg white lysozyme accounts for the 3.4%~3.5% of egg white total protein, is that current research and application are most
Lysozyme.White, slightly sweet, odorless, soluble easily in water characteristic, in acid condition thermostabilization is presented in egg white lysozyme freeze-dried powder
Property is very strong.Lysozyme is widely used in the anti-corrosion of food mainly as preservative at present, and safety is good.
In summary, the developing target market that lysozyme is applied to oral-cavity article has a high potential.However, in conventional oral-cavity article
Essential foaming agent, binder, sweetener, chemical preservative etc. can cause bacteriolyze enzyme denaturation in various degree, thus
Cause its active reduction or loss.Therefore, effective protection lysozyme activity is its key points and difficulties for being applied to oral-cavity article
One of.
Summary of the invention
The purpose of the invention is to overcome the deficiencies of the prior art and provide a kind of toothpaste containing biological lysozyme.
To achieve the goals above, the present invention is achieved by the following technical programs：
A kind of toothpaste containing biological lysozyme contains the biological lysozyme compositions of additives of 0.1~15% (wt), institute
Biological lysozyme compositions of additives is stated to be grouped as by each group of following weight percent：Biological lysozyme 10~40%, it is modified
Natural plant gum 0.01~2.0%, EDETATE SODIUM 0.01~0.3%, deionized water surplus.
Preferably, the biological lysozyme compositions of additives is grouped as by each group of following weight percent：Biology is molten
Bacterium enzyme 20~30%, modified plant gum 0.1~1.0%, EDETATE SODIUM 0.1~0.3%, deionized water surplus.
Preferably, the toothpaste contains each component of following weight percent：Biological lysozyme compositions of additives 0.1~
15%, moisturizer 10~70%, rubbing agent 10~50%, thickener 0.01~3%, foaming agent 1~3%, sweetener 0.01~
1%, essence 0.1~2%.
It is highly preferred that the toothpaste contains each component of following weight percent：Biological lysozyme compositions of additives
3.75~15%, moisturizer 40~65%, rubbing agent 15~20%, thickener 0.7~1.2%, foaming agent 1.5~2.5%, sweet tea
Taste agent 0.25%, essence 1%.
Most preferably, the toothpaste contains each component of following weight percent：Biological lysozyme compositions of additives
6%, moisturizer 10~70%, rubbing agent 10~50%, thickener 0.01~3%, foaming agent 1~3%, sweetener 0.01~
1%, essence 0.1~2%；The biology lysozyme compositions of additives is grouped as by each group of following weight percent：Biology
Lysozyme 25%, carrageenan 0.5%, EDETATE SODIUM 0.2%, deionized water surplus.
Most preferably, the toothpaste contains each component of following weight percent：Biological lysozyme compositions of additives
6%, moisturizer 40~65%, rubbing agent 15~20%, thickener 0.7~1.2%, foaming agent 1.5~2.5%, sweetener
0.25%, essence 1%；The biology lysozyme compositions of additives is grouped as by each group of following weight percent：Biology is molten
Bacterium enzyme 25%, carrageenan 0.5%, EDETATE SODIUM 0.2%, deionized water surplus.
Most preferably, the toothpaste contains each component of following weight percent：Biological lysozyme compositions of additives
3.75%, moisturizer 10~70%, rubbing agent 10~50%, thickener 0.01~3%, foaming agent 1~3%, sweetener 0.01
~1%, essence 0.1~2%；The biology lysozyme compositions of additives is grouped as by each group of following weight percent：It is raw
Object lysozyme 40%, guar gum 0.1%, carrageenan 0.7%, EDETATE SODIUM 0.3%, deionized water surplus.
Most preferably, the toothpaste contains each component of following weight percent：Biological lysozyme compositions of additives
3.75, moisturizer 40~65%, rubbing agent 15~20%, thickener 0.7~1.2%, foaming agent 1.5~2.5%, sweetener
0.25%, essence 1%；The biology lysozyme compositions of additives is grouped as by each group of following weight percent：Biology is molten
Bacterium enzyme 40%, guar gum 0.1%, carrageenan 0.7%, EDETATE SODIUM 0.3%, deionized water surplus.
Most preferably, the toothpaste is grouped as by each group of following weight percent：Biological lysozyme compositions of additives
3.75~15%, sorbierite 25%, glycerol 20%, rubbing agent 20%, sodium carboxymethylcellulose 0.8%, laruyl alcohol sodium sulfovinate
2%, sweetener 0.25%, essence 1%, deionized water surplus.
Most preferably, the toothpaste is grouped as by each group of following weight percent：Biological lysozyme compositions of additives
5%, sorbierite 60%, glycerol 5%, rubbing agent 15%, sodium carboxymethylcellulose 0.7%, laruyl alcohol sodium sulfovinate 1.5%, the moon
Osmanthus acylsarcosine sodium 1.0%, sodium phytate 1.0%, sweetener 0.25%, essence 1%, deionized water surplus.
Most preferably, the toothpaste is grouped as by each group of following weight percent：Biological lysozyme compositions of additives
3.75~15%, sorbierite 20%, glycerol 10%, polyethylene glycol-400 10%, rubbing agent 20%, sodium carboxymethylcellulose
0.8%, xanthan gum 0.3%, laruyl alcohol sodium sulfovinate 2%, sodium fluoride 0.32%, sweetener 0.25%, essence 1%, deionization
Water surplus.
Most preferably, the toothpaste is grouped as by each group of following weight percent：Biological lysozyme compositions of additives
5%, sorbierite 30%, glycerol 10%, polyethylene glycol -6005%, rubbing agent 15%, hydroxyapatite 5%, carboxymethyl cellulose
Sodium 0.7%, xanthan gum 0.5%, laruyl alcohol sodium sulfovinate 1.5%, potassium nitrate 3.0%, strontium chloride 0.3%, sweetener 0.25%,
Essence 1%, deionized water surplus.
Compared with prior art, the present invention has the advantages that：
Toothpaste provided by the invention uses a kind of biological lysozyme compositions of additives of special formulation, the composition
It uses, can make the biological lysozyme being added in toothpaste from substances such as foaming agent, binder, sweetener, preservatives to molten
The denaturation of bacterium enzyme influences, so that biological lysozyme activity be prevented not to be destroyed or reduce.Therefore, toothpaste provided by the invention can
The Escherichia coli for causing the oral problems such as canker sore, gingivitis, implication halitosis and staphylococcus aureus etc. are killed in efficient suppression.
Specific embodiment
The present invention is further elaborated combined with specific embodiments below, the embodiment is served only for explaining this hair
It is bright, it is not intended to limit the scope of the present invention.Test method as used in the following examples is routine unless otherwise specified
Method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
Embodiment 1
A kind of compositions of additives containing biological lysozyme, formula are matched for any one in the formula 1~14 in table 1
Side.
The preparation method of compositions of additives containing biological lysozyme is：First EDETATE SODIUM, modified plant gum are dissolved in
In deionized water, it is dispersed with stirring dissolution, improved plant glue solution is made；Above-mentioned improved plant glue is added in biological lysozyme
In solution, be slowly stirred dispersing and dissolving to get.
Later referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, tests and be formulated 1~14 in table 1
Composition lysozyme activity.Before test, first by the compositions of additives containing biological lysozyme of formula 1~14 in table 1
First 1 is pressed with deionized water：100 ratio wiring solution-formings dilute 500 times with deionized water later, are then detected again, and same
Walk the contrast test lysozyme activity of corresponding content biology lysozyme.As a result following Tables 1 and 2.
Table 1 is the composition for being formulated 1~14
The lysozyme activity of the corresponding content biology lysozyme of table 2
| Ingredient | Formula 1 | Formula 2 | Formula 3 | Formula 4 | Formula 5 | Formula 6 |
| Biological lysozyme | 10 | 15 | 20 | 25 | 30 | 40 |
| Deionized water | 90 | 85 | 80 | 75 | 70 | 60 |
| It is total | 100% | 100% | 100% | 100% | 100% | 100% |
| Lysozyme activity | 6501 | 9912 | 12815 | 20900 | 24000 | 29789 |
By the result of Tables 1 and 2 it is found that compositions of additives does not have negatively influencing to lysozyme enzymatic activity.
Application examples 1
It is applied to 1~6 corresponding compositions of additives containing biological lysozyme is formulated in 1 table 1 of embodiment in toothpaste,
The specific formula of toothpaste is shown in Table 3.
Referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, the corresponding toothpaste of measurement application examples 1~6
Lysozyme activity.10 times of deionized water of toothpaste is diluted, is dispersed with stirring uniformly about 30 minutes, centrifuging and taking supernatant.It will
Supernatant is detected after diluting 500 times with deionized water.
Referring to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB 15979-2002C4
Stripping property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding toothpaste of measurement application examples 1~6 is to Escherichia coli, golden yellow Portugal
Grape coccus, porphyromonas gingivalis, streptococcus mutans inhibiting effect.And synchronism detection control 1, control 1 is i.e. according to tradition side
Biological lysozyme is directly added into toothpaste by method, measurement result such as table 3.
Table 3
Note：Application examples 1~6 and the content of the biological lysozyme in control 1 are identical in table 3.
It can be seen that being applied to containing biological lysozyme compositions of additives by different ratio using result according to table 3
In toothpaste, the enzymatic activity and bacteriostasis rate of biological lysozyme are than individually adding enzymatic activity and suppression of the biological lysozyme raw material in toothpaste
Bacterium rate improves, and has significant difference.The present invention is by biological lysozyme and modified plant gum, EDETATE SODIUM, deionized water
It is combined according to specific proportion, can protect biological lysozyme from the influence of other compositions in toothpaste, protection biology
The activity of lysozyme.
In addition, can also be seen that lysozyme content is consistent in control application examples from the result of application examples in table 3, addition is not
With the composition of proportion, it is found that the composition degree that lysozyme activity changes in application examples of different ratio is inconsistent.Therefore,
It can be seen that modified plant gum type and dosage, the content of EDETATE DISODIUM will affect composition and be applied in product in composition
Lysozyme activity.This illustrates that different types of modified plant gum and different amounts of EDETATE SODIUM protect biological lysozyme activity
Degree is different.Identical using EDETATE SODIUM additive amount such as application examples 1 and 3, the modified plant gum content of application examples 1, which is higher than, answers
The content of modified adhesive in use-case 3, and the antalzyme activity of application examples 3 changes degree and is slightly above the change of 1 antalzyme activity of application examples
Degree, it is preferable than hydroxyethyl cellulose effect is used alone that this explanation is used in compounding sodium alginate and guar gum modified plant gum.
Application examples 2 and 5 is compared, it is identical with modified plant gum type using EDETATE SODIUM additive amount, and the antalzyme activity of application examples 5 changes
Change degree is higher than 2 antalzyme activity of application examples and changes degree, this is because 5 locust bean gum additive amount of application examples is higher than application examples 2.
This illustrates that the increase of improved plant glue amount has better protection to lysozyme activity.Application examples 3 and 4 is compared, application examples 3 uses modification
Plant glue amount is higher than application examples 4, and 3 lysozyme activity of application examples is far below application examples 4, this illustrates EDETATE SODIUM additive amount to molten
Bacterium enzymatic activity retentivity influence degree is big, and lysozyme activity increases with EDETATE SODIUM additive amount and enhanced.Compare 1 He of application examples
4 can also illustrate this point.
Application examples 2
Toothpaste is applied to by 7~14 corresponding compositions of additives containing biological lysozyme are formulated in 1 table 1 of embodiment
In, the specific formula of toothpaste is shown in Table 4.
Referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, the corresponding toothpaste of measurement application examples 1~8
Lysozyme activity.10 times of deionized water of toothpaste is diluted, is dispersed with stirring uniformly about 30 minutes, centrifuging and taking supernatant.It will
Supernatant is detected after diluting 500 times with deionized water.
Referring to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB 15979-2002C4
Stripping property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding toothpaste of measurement application examples 1~8 is to Escherichia coli, golden yellow Portugal
Grape coccus, porphyromonas gingivalis, streptococcus mutans inhibiting effect.And synchronism detection control 1, control 1 is i.e. according to tradition side
Biological lysozyme is directly added into toothpaste by method, measurement result such as table 4.
Table 4
Note：Application examples 1~8 and the content of the biological lysozyme in control 1 are identical in table 4.
Application examples 3
It is applied to 1~6 corresponding compositions of additives containing biological lysozyme is formulated in 1 table 1 of embodiment in toothpaste,
The specific formula of toothpaste is shown in Table 5.
Referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, the corresponding toothpaste of measurement application examples 1~6
Lysozyme activity.10 times of deionized water of toothpaste is diluted, is dispersed with stirring uniformly about 30 minutes, centrifuging and taking supernatant.It will
Supernatant is detected after diluting 500 times with deionized water.
Referring to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB 15979-2002C4
Stripping property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding toothpaste of measurement application examples 1~6 is to Escherichia coli, golden yellow Portugal
Grape coccus, porphyromonas gingivalis, streptococcus mutans inhibiting effect.And synchronism detection control 1, control 1 is i.e. according to tradition side
Biological lysozyme is directly added into toothpaste by method, measurement result such as table 5.
Table 5
Note：Application examples 1~6 and the content of the biological lysozyme in control 1 are identical in table 5.
Application examples 4
Toothpaste is applied to by 7~14 corresponding compositions of additives containing biological lysozyme are formulated in 1 table 1 of embodiment
In, the specific formula of toothpaste is shown in Table 6.
Referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, the corresponding toothpaste of measurement application examples 1~8
Lysozyme activity.10 times of deionized water of toothpaste is diluted, is dispersed with stirring uniformly about 30 minutes, centrifuging and taking supernatant.It will
Supernatant is detected after diluting 500 times with deionized water.
Referring to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB 15979-2002C4
Stripping property resists (suppression) bacterium product bacteriostasis property test method, and the corresponding toothpaste of measurement application examples 1~8 is to Escherichia coli, golden yellow Portugal
Grape coccus, porphyromonas gingivalis, streptococcus mutans inhibiting effect.And synchronism detection control 1, control 1 is i.e. according to tradition side
Biological lysozyme is directly added into toothpaste by method, measurement result such as table 6.
Table 6
Note：Application examples 1~8 and the content of the biological lysozyme in control 1 are identical in table 6.
Biological lysozyme compositions of additives of the present invention by addition special formulation in the toothpaste to different formulations, toothpaste
The activity of middle biology lysozyme does not reduce, this illustrates that biological lysozyme compositions of additives can be added to any formula
In toothpaste, therefore, although the present invention is only illustrated with the toothpaste of four kinds of different formulations, this does not represent biological bacteriolyze
Enzyme additive composition can be only used in the toothpaste of above-mentioned four kinds of different formulations.
Claims (10)
1. a kind of toothpaste containing biological lysozyme, which is characterized in that contain the biological bacteriolyze enzyme additive of 0.1~15% (wt)
Composition, the biology lysozyme compositions of additives are grouped as by each group of following weight percent：Biological lysozyme 10~
40%, modified plant gum 0.01~2.0%, EDETATE SODIUM 0.01~0.3%, deionized water surplus；The modified plant gum is
Guar gum, locust bean gum, carrageenan, hydroxyethyl cellulose, any one or two kinds in sodium alginate.
2. toothpaste according to claim 1, which is characterized in that the biology lysozyme compositions of additives is by following weight
The each group of percentage is grouped as：Biological lysozyme 20~30%, modified plant gum 0.1~1.0%, EDETATE SODIUM 0.1~
0.3%, deionized water surplus.
3. toothpaste according to claim 1, which is characterized in that the toothpaste contains each component of following weight percent：
Biological lysozyme compositions of additives 0.1~15%, moisturizer 10~70%, rubbing agent 10~50%, thickener 0.01~
3%, foaming agent 1~3%, sweetener 0.01~1%, essence 0.1~2%.
4. toothpaste according to claim 1, which is characterized in that the toothpaste contains each component of following weight percent：
Biological lysozyme compositions of additives 3.75~15%, moisturizer 40~65%, rubbing agent 15~20%, thickener 0.7~
1.2%, foaming agent 1.5~2.5%, sweetener 0.25%, essence 1%.
5. toothpaste according to claim 3 or 4, which is characterized in that the biology lysozyme compositions of additives is by following
The each group of weight percent is grouped as：Biological lysozyme 25%, carrageenan 0.5%, EDETATE SODIUM 0.2%, more than deionized water
Amount；Dosage of the biological lysozyme compositions of additives in toothpaste is 6%.
6. toothpaste according to claim 3 or 4, which is characterized in that the biology lysozyme compositions of additives is by following
The each group of weight percent is grouped as：Biological lysozyme 40%, guar gum 0.1%, carrageenan 0.7%, EDETATE SODIUM
0.3%, deionized water surplus；Dosage of the biological lysozyme compositions of additives in toothpaste is 3.75%.
7. toothpaste according to claim 1, which is characterized in that the toothpaste is grouped by each group of following weight percent
At：Biological lysozyme compositions of additives 3.75~15%, sorbierite 25%, glycerol 20%, rubbing agent 20%, carboxymethyl cellulose
Plain sodium 0.8%, laruyl alcohol sodium sulfovinate 2%, sweetener 0.25%, essence 1%, deionized water surplus.
8. toothpaste according to claim 1, which is characterized in that the toothpaste is grouped by each group of following weight percent
At：Biological lysozyme compositions of additives 5%, sorbierite 60%, glycerol 5%, rubbing agent 15%, sodium carboxymethylcellulose
0.7%, laruyl alcohol sodium sulfovinate 1.5%, sodium lauroyl sarcosine 1.0%, sodium phytate 1.0%, sweetener 0.25%, essence
1%, deionized water surplus.
9. toothpaste according to claim 1, which is characterized in that the toothpaste is grouped by each group of following weight percent
At：Biological lysozyme compositions of additives 3.75~15%, sorbierite 20%, glycerol 10%, polyethylene glycol-400 10% are rubbed
Liniment 20%, sodium carboxymethylcellulose 0.8%, xanthan gum 0.3%, laruyl alcohol sodium sulfovinate 2%, sodium fluoride 0.32%, sweet taste
Agent 0.25%, essence 1%, deionized water surplus.
10. toothpaste according to claim 1, which is characterized in that the toothpaste is grouped by each group of following weight percent
At：Biological lysozyme compositions of additives 5%, sorbierite 30%, glycerol 10%, polyethylene glycol -600 5%, rubbing agent 15%,
Hydroxyapatite 5%, sodium carboxymethylcellulose 0.7%, xanthan gum 0.5%, laruyl alcohol sodium sulfovinate 1.5%, potassium nitrate
3.0%, strontium chloride 0.3%, sweetener 0.25%, essence 1%, deionized water surplus.
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| 溶菌酶与EDTA二钠的协同抑菌作用;李小曼;《北京大学学报（医学版）》;20150228;第47卷(第1期);全文 * |
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