CN107582419B - A kind of mouthwash containing biological lysozyme - Google Patents
A kind of mouthwash containing biological lysozyme Download PDFInfo
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- CN107582419B CN107582419B CN201710922534.3A CN201710922534A CN107582419B CN 107582419 B CN107582419 B CN 107582419B CN 201710922534 A CN201710922534 A CN 201710922534A CN 107582419 B CN107582419 B CN 107582419B
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- lysozyme
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- 235000010335 lysozyme Nutrition 0.000 title claims abstract description 91
- 102000016943 Muramidase Human genes 0.000 title claims abstract description 90
- 108010014251 Muramidase Proteins 0.000 title claims abstract description 90
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 title claims abstract description 90
- 229960000274 lysozyme Drugs 0.000 title claims abstract description 89
- 239000004325 lysozyme Substances 0.000 title claims abstract description 89
- 239000002324 mouth wash Substances 0.000 title claims abstract description 50
- 229940051866 mouthwash Drugs 0.000 title claims abstract description 49
- 239000000203 mixture Substances 0.000 claims abstract description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000000654 additive Substances 0.000 claims abstract description 32
- 239000008367 deionised water Substances 0.000 claims abstract description 23
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 23
- 235000003599 food sweetener Nutrition 0.000 claims abstract description 9
- 239000003765 sweetening agent Substances 0.000 claims abstract description 9
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims abstract description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 239000002826 coolant Substances 0.000 claims description 9
- 239000004909 Moisturizer Substances 0.000 claims description 6
- 239000003995 emulsifying agent Substances 0.000 claims description 6
- 230000001333 moisturizer Effects 0.000 claims description 6
- 229920000161 Locust bean gum Polymers 0.000 claims description 5
- 235000010420 locust bean gum Nutrition 0.000 claims description 5
- 239000000711 locust bean gum Substances 0.000 claims description 5
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 4
- 235000010413 sodium alginate Nutrition 0.000 claims description 4
- 239000000661 sodium alginate Substances 0.000 claims description 4
- 229940005550 sodium alginate Drugs 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 23
- 241000894006 Bacteria Species 0.000 abstract description 13
- 238000009472 formulation Methods 0.000 abstract description 6
- 239000003755 preservative agent Substances 0.000 abstract description 5
- 206010006326 Breath odour Diseases 0.000 abstract description 4
- 208000032139 Halitosis Diseases 0.000 abstract description 4
- 208000007565 gingivitis Diseases 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 239000011230 binding agent Substances 0.000 abstract description 3
- 208000020670 canker sore Diseases 0.000 abstract description 3
- 238000004925 denaturation Methods 0.000 abstract description 3
- 230000036425 denaturation Effects 0.000 abstract description 3
- 239000004088 foaming agent Substances 0.000 abstract description 3
- 210000000214 mouth Anatomy 0.000 description 18
- 241000196324 Embryophyta Species 0.000 description 12
- 238000000034 method Methods 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 8
- 102000002322 Egg Proteins Human genes 0.000 description 8
- 108010000912 Egg Proteins Proteins 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 8
- 235000014103 egg white Nutrition 0.000 description 8
- 210000000969 egg white Anatomy 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 5
- 241001478240 Coccus Species 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- 241000219095 Vitis Species 0.000 description 5
- 235000009754 Vitis X bourquina Nutrition 0.000 description 5
- 235000012333 Vitis X labruscana Nutrition 0.000 description 5
- 235000014787 Vitis vinifera Nutrition 0.000 description 5
- 230000001629 suppression Effects 0.000 description 5
- 238000010998 test method Methods 0.000 description 5
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 4
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 4
- 241000605862 Porphyromonas gingivalis Species 0.000 description 4
- 241000194019 Streptococcus mutans Species 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 230000001360 synchronised effect Effects 0.000 description 4
- CTKXFMQHOOWWEB-UHFFFAOYSA-N Ethylene oxide/propylene oxide copolymer Chemical compound CCCOC(C)COCCO CTKXFMQHOOWWEB-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 235000010418 carrageenan Nutrition 0.000 description 3
- 239000000679 carrageenan Substances 0.000 description 3
- 229920001525 carrageenan Polymers 0.000 description 3
- 229940113118 carrageenan Drugs 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000005548 health behavior Effects 0.000 description 3
- 229940044519 poloxamer 188 Drugs 0.000 description 3
- 229920001993 poloxamer 188 Polymers 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical group [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 3
- FTLYMKDSHNWQKD-UHFFFAOYSA-N (2,4,5-trichlorophenyl)boronic acid Chemical compound OB(O)C1=CC(Cl)=C(Cl)C=C1Cl FTLYMKDSHNWQKD-UHFFFAOYSA-N 0.000 description 2
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 2
- 239000000022 bacteriostatic agent Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 2
- 230000000116 mitigating effect Effects 0.000 description 2
- 208000030194 mouth disease Diseases 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 229940085605 saccharin sodium Drugs 0.000 description 2
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000811 xylitol Substances 0.000 description 2
- 235000010447 xylitol Nutrition 0.000 description 2
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 2
- 229960002675 xylitol Drugs 0.000 description 2
- 241001474374 Blennius Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- OYWXRWURAJWYMS-UHFFFAOYSA-N N1C=CC=C1.[Cl].N Chemical compound N1C=CC=C1.[Cl].N OYWXRWURAJWYMS-UHFFFAOYSA-N 0.000 description 1
- XEFQLINVKFYRCS-UHFFFAOYSA-N Triclosan Chemical compound OC1=CC(Cl)=CC=C1OC1=CC=C(Cl)C=C1Cl XEFQLINVKFYRCS-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000002272 anti-calculus Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000013368 commensalism Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000012969 defense response to bacterium Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 210000003298 dental enamel Anatomy 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940124274 edetate disodium Drugs 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 229940051921 muramidase Drugs 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000003239 periodontal effect Effects 0.000 description 1
- 201000001245 periodontitis Diseases 0.000 description 1
- 210000004261 periodontium Anatomy 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- -1 phosphoric acid hydrogen Chemical class 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 235000013024 sodium fluoride Nutrition 0.000 description 1
- 239000011775 sodium fluoride Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 235000011008 sodium phosphates Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
- 229960003500 triclosan Drugs 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
Abstract
The invention discloses a kind of mouthwashes containing biological lysozyme, the mouthwash contains 0.1~15%(wt) biological lysozyme compositions of additives, the biology lysozyme compositions of additives is grouped as by each group of following weight percent: biological lysozyme 10~40%, modified plant gum 0.01~2.0%, EDETATE SODIUM 0.01~0.3%, deionized water surplus.Mouthwash provided by the invention uses a kind of biological lysozyme compositions of additives of special formulation, the use of the composition can be such that the biological lysozyme being added in mouthwash influences from substances such as foaming agent, binder, sweetener, preservatives on the denaturation of lysozyme, so that biological lysozyme activity be prevented not to be destroyed or reduce.Therefore, mouthwash provided by the invention can effectively press down the harmful bacteria for killing and causing a variety of oral problems such as canker sore, gingivitis, implication halitosis.
Description
Technical field
The present invention relates to wash shield product technical field, and in particular, to a kind of mouthwash containing biological lysozyme.
Background technique
The oral cavity of people is an intact ecocystem, is provided to the growth and breeding and colonization of microorganisms various in oral cavity
The environment and condition being particularly suitable for.In oral cavity the microorganism of field planting include the bacterium of type complexity, fungi, mycoplasma, protozoon and
Virus, these microorganisms constitute oral microecosystem in oral cavity different parts commensalism competition and antagonism, strong with host buccal
Health and disease close relation.Oral health behaviours imbalance factor be it is complicated, can be age, diet, health, health status
And use of antibiotic etc., it is also possible to the influence to interact between microorganism, and eventually lead to microbial state treatment.Oral cavity
Disease causes Oral health behaviours to be lacked of proper care, otherwise Oral health behaviours imbalance also can produce mouth disease.Plaque is to cause periodontal
One of most important factor of the odontopathies such as disease, saprodontia.The growth, breeding of bacterium, production acid, make enamel decalcification in bacterial plaque,
Lead to the formation of saprodontia.Plaque has very strong adhesiveness, is not easy to thoroughly remove.Bacterial plaque control is exactly to inhibit, or even remove
Bacterial plaque, and prevent bacterial plaque from being formed again on facing.The key of bacterial plaque control is to control oral cavity harmful bacteria regularly, protect
Bacteriostatic agent used in holding is effective for a long time.Since the growth of bacterial plaque is continuously, so the oral cavity of effective bacteriostatic agent is cooperated to protect
Managing liquid is a kind of easy, effective, practical bacterial plaque control method.Oral cavity cleaning and nursing liquid includes mouthwash, mouth spraying agent etc., master
It acts on and is to clean oral cavity, cover oral cavity bad smell, and make in mouth that there are the feelings of fresh and comfortable.On this basis, increase
It is added in water constituent of gargling and the ingredient with bacteriostasis is added, increase book bacteriostasis efficacy of gargling, plaque in oral cavity can be inhibited
It is formed, the risk that various mouth diseases generate is reduced, to preferably take good care of oral health.At present on the market it is common it is antibacterial at
Dividing is the ingredients such as antibiotic, chlorohexidene, western pyrrole chlorine ammonia, triclosan, Chinese herbal medicine, preservative.These ingredient one kind are antibiotic, long
Phase use is also easy to produce drug resistance；Second is that chemical reagent class, mouthfeel is puckery, bitter, can also want dental stain, and existing document report is deposited
In suspicious carcinogenesis；Third is that Chinese herbal medicine or plant extracts, this substance source raw material active constituent is few, and cost is expensive, face
Color depth, use is constant, is easily oxidized, and especially the mechanism of action is unknown, and how unknown effect is.
Biology lysozyme of the present invention is a kind of natural antibacterial albumen, is widely present in water, animal point
In bleeding, tear, saliva, milk, birds, beasts and eggs and part bacterium.Lysozyme not only has muramidase activity, can hydrolyze peptide glycan
In -acetylmuramic acid and N-Acetyl-D-glucosamine between β-Isosorbide-5-Nitrae glycosidic bond, but also have antibacterial peptide activity, this with it is molten
The structure of bacterium enzyme, distribution of charges and surface hydrophobic are related.Egg white lysozyme accounts for the 3.4%~3.5% of egg white total protein, is mesh
It is preceding to study and apply most lysozymes.White, slightly sweet, odorless, soluble easily in water characteristic is presented in egg white lysozyme freeze-dried powder,
Thermal stability is very strong under acid condition.Lysozyme is widely used in the anti-corrosion of food, safety mainly as preservative at present
It is good.Mouthwash is carrier to the present invention, and biological lysozyme is applied in oral care product, has and inhibits plaque in oral cavity
Formation effect, can effectively inhibit the harmful bacterial growth for causing the oral problems such as canker sore, gingivitis, implication halitosis,
A variety of oral problems can be improved, mitigate gingivitis bleeding gums, prevention and mitigation periodontitis, promote the health of periodontium, have
There are antibacterial, anti-calculus dentalis, preventing decayed tooth, mitigation halitosis and other effects.
In summary, the developing target market that lysozyme is applied to oral-cavity article has a high potential.However, in conventional oral-cavity article
Essential foaming agent, binder, sweetener, chemical preservative etc. can cause bacteriolyze enzyme denaturation in various degree, thus
Cause its active reduction or loss.Therefore, effective protection lysozyme activity is its key points and difficulties for being applied to oral-cavity article
One of.
Summary of the invention
The purpose of the invention is to overcome the deficiencies of the prior art and provide a kind of mouthwash containing biological lysozyme.
To achieve the goals above, the present invention is achieved by the following technical programs:
A kind of mouthwash containing biological lysozyme contains the biological lysozyme compositions of additives of 0.1~15% (wt),
The biology lysozyme compositions of additives is grouped as by each group of following weight percent: biological lysozyme 10~40% changes
Property natural plant gum 0.01~2.0%, EDETATE SODIUM 0.01~0.3%, deionized water surplus.
Preferably, the biological lysozyme compositions of additives is grouped as by each group of following weight percent: biology is molten
Bacterium enzyme 20~30%, modified plant gum 0.1~1.0%, EDETATE SODIUM 0.1~0.3%, deionized water surplus.
Preferably, the mouthwash contains each component of following weight percent, biological lysozyme compositions of additives 0.1
~15%, moisturizer 1~30%, emulsifier 0.01~2%, sweetener 0.001~1%, essence 0.01~1%, coolant agent
0.001~0.2%.
It is highly preferred that the mouthwash contains each component of following weight percent, biological lysozyme compositions of additives
3.75~15%, moisturizer 19~25%, emulsifier 1%, sweetener 0.05~1%, essence 0.2%, coolant agent 0.05%.
Most preferably, the mouthwash contains each component of following weight percent, biological lysozyme compositions of additives
5%, moisturizer 1~30%, emulsifier 0.01~2%, sweetener 0.001~1%, essence 0.01~1%, coolant agent 0.001
~0.2%；The biology lysozyme compositions of additives is grouped as by each group of following weight percent: biological lysozyme
20%, locust bean gum or sodium alginate 0.1%, EDETATE SODIUM 0.2%, deionized water surplus.
Most preferably, the mouthwash contains each component of following weight percent, biological lysozyme compositions of additives
5%, moisturizer 19~25%, emulsifier 1%, sweetener 0.05~1%, essence 0.2%, coolant agent 0.05%；The biology
Lysozyme compositions of additives is grouped as by each group of following weight percent: biological lysozyme 20%, locust bean gum or seaweed
Sour sodium 0.1%, EDETATE SODIUM 0.2%, deionized water surplus.
Most preferably, the mouthwash is grouped as by each group of following weight percent: biological bacteriolyze enzyme additive combines
Object 5%, sorbierite 15%, PEG4005%, propylene glycol 5%, rilanit special 1%, xylitol 0.5%, sodium phosphate 0.05%,
Essence 0.2%, coolant agent 0.05%, deionized water surplus.
Most preferably, the mouthwash is grouped as by each group of following weight percent: biological bacteriolyze enzyme additive combines
Object 3.75~15%, sorbierite 5%, glycerol 10%, propylene glycol 5%, poloxamer188 1%, sodium fluoride 0.1%, saccharin sodium
0.05%, disodium hydrogen phosphate 0.02%, citric acid 0.01%, essence 0.2%, coolant agent 0.05%, deionized water surplus.
Most preferably, the mouthwash is grouped as by each group of following weight percent: biological bacteriolyze enzyme additive combines
Object 5%, sorbierite 8%, glycerol 6%, propylene glycol 5%, poloxamer188 1%, xylitol 1%, disodium hydrogen phosphate 0.02%,
Sodium dihydrogen phosphate 0.02%, essence 0.2%, coolant agent 0.05%, deionized water surplus.
Most preferably, the mouthwash is grouped as by each group of following weight percent: biological bacteriolyze enzyme additive combines
Object 3.75~15%, sorbierite 12%, glycerol 6%, PEG4003%, poloxamer188 1%, saccharin sodium 0.05%, phosphoric acid hydrogen
Disodium 0.03%, citric acid 0.01%, essence 0.2%, coolant agent 0.05%, deionized water surplus.
Compared with prior art, the invention has the following beneficial effects:
Mouthwash provided by the invention uses a kind of biological lysozyme compositions of additives of special formulation, the composition
Use, the biological lysozyme being added in mouthwash can be made from substances such as foaming agent, binder, sweetener, preservatives
The denaturation of lysozyme is influenced, so that biological lysozyme activity be prevented not to be destroyed or reduce.Therefore, provided by the invention to gargle
The Escherichia coli for causing the oral problems such as canker sore, gingivitis, implication halitosis and golden yellow grape are killed in the enough efficient suppressions of water energy
Coccus etc..
Specific embodiment
The present invention is made combined with specific embodiments below and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method as used in the following examples is normal unless otherwise specified
Rule method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
Embodiment 1
A kind of compositions of additives containing biological lysozyme, formula are matched for any one in the formula 1~14 in table 1
Side.
Compositions of additives containing biological lysozyme the preparation method comprises the following steps: first EDETATE SODIUM, modified plant gum are dissolved in
In deionized water, it is dispersed with stirring dissolution, improved plant glue solution is made；Above-mentioned improved plant glue is added in biological lysozyme
In solution, be slowly stirred dispersing and dissolving to get.
Later referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, tests and be formulated 1~14 in table 1
Composition lysozyme activity.Before test, first by the compositions of additives containing biological lysozyme of formula 1~14 in table 1
First with deionized water in 1:100 ratio wiring solution-forming, 500 times are diluted with deionized water later, is then detected again, and is same
Walk the contrast test lysozyme activity of corresponding content biology lysozyme.As a result following Tables 1 and 2.
Table 1 is the composition for being formulated 1~14
The lysozyme activity of the corresponding content biology lysozyme of table 2
| Ingredient | Formula 1 | Formula 2 | Formula 3 | Formula 4 | Formula 5 | Formula 6 |
| Biological lysozyme | 10 | 15 | 20 | 25 | 30 | 40 |
| Deionized water | 90 | 85 | 80 | 75 | 70 | 60 |
| It is total | 100% | 100% | 100% | 100% | 100% | 100% |
| Lysozyme activity | 6501 | 9912 | 12815 | 20900 | 24000 | 29789 |
By the result of Tables 1 and 2 it is found that compositions of additives does not have negatively influencing to lysozyme enzymatic activity.
Application examples 1
7~14 corresponding compositions of additives containing biological lysozyme will be formulated in 1 table 1 of embodiment to be added to gargling
In water.The formula of each mouthwash is shown in Table the application examples 1~8 in 3.
Referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, the measurement correspondence of application examples 1~8 is gargled
The lysozyme activity of water.10 times of deionized water of mouthwash is diluted, centrifuging and taking supernatant dilutes 500 with deionized water later
It is detected after times.
Referring to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB 15979-2002C4
Stripping property resists (suppression) bacterium product bacteriostasis property test method, measures the mouthwash of application examples 1~8 to Escherichia coli, golden yellow Portugal
Grape coccus, porphyromonas gingivalis, Streptococcus mutans inhibiting effect.And synchronous setting control 1, control 1 is according to tradition side
Biological lysozyme is directly added into mouthwash by method, measurement result such as table 3.
Table 3
Note: application examples 1~8 and the content of the biological lysozyme of control 1 are identical in table 3.
It can be seen that being applied to containing biological lysozyme compositions of additives by different ratio using result according to table 3
In mouthwash, enzymatic activity and bacteriostasis rate are mentioned than individually adding enzymatic activity and bacteriostasis rate of the biological lysozyme raw material in mouthwash
Height, and there is significant difference.The present invention is by biological lysozyme with modified plant gum, EDETATE SODIUM, deionized water according to specific
Proportion combine, can protect biological lysozyme from the influence of other compositions in mouthwash, protect biological lysozyme
Activity.It should be noted that when, the amount addition of modified plant gum is excessive, can have a negative impact to mouthwash mouthfeel.
In addition, can also be seen that lysozyme content is consistent in control application examples from the result of application examples in table 3, addition is not
With the composition of proportion, it is found that the composition degree that lysozyme activity changes in application examples of different ratio is inconsistent.Therefore,
It can be seen that in composition modified plant gum type and the content of EDETATE DISODIUM will affect composition be applied to it is molten in product
Bacterium enzymatic activity.This illustrates different types of modified plant gum and different amounts of EDETATE SODIUM to biological lysozyme activity degree of protection
It is different.For example, although the Comparative result of application examples 1 and 4 can be seen that the hydroxyethyl cellulose that application examples 1 uses from table 3
Amount be greater than the amount of the sodium alginate that application examples 4 uses, but from the point of view of antalzyme activity changes situation, application examples 4 it is molten
Bacterium enzyme activity improves degree and is better than application examples 1, therefore, it can be seen that sodium alginate is better than the protective effect of biological lysozyme
Hydroxyethyl cellulose.Application examples 1 and application examples 2 use same amount of modified plant gum, but the antalzyme activity of application examples 1 mentions
High level is better than application examples 2, and reason is that the modified plant gum that application examples 1 uses is hydroxyethyl cellulose, what application examples 2 used
Modified plant gum is carrageenan.Although the carrageenan that the amount and application examples 2 of the hydroxyethyl cellulose that application examples 1 uses use
Amount be greater than the amount of locust bean gum that application examples 3 uses, but from the point of view of antalzyme activity changes situation, the bacteriolyze of application examples 3
Enzyme activity improves degree and is significantly better than application examples 1 and 2, this illustrates that locust bean gum is better than hydroxyl to the protective effect of biological lysozyme
Ethyl cellulose, carrageenan.
Application examples 2
1~6 corresponding compositions of additives containing biological lysozyme will be formulated in 1 table 1 of embodiment to be added to gargling
In water.The formula of each mouthwash is shown in Table the application examples 1~6 in 4.
Referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, the measurement correspondence of application examples 1~6 is gargled
The lysozyme activity of water.10 times of deionized water of mouthwash is diluted, centrifuging and taking supernatant dilutes 500 with deionized water later
It is detected after times.
Referring to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB 15979-2002C4
Stripping property resists (suppression) bacterium product bacteriostasis property test method, measures the mouthwash of application examples 1~6 to Escherichia coli, golden yellow Portugal
Grape coccus, porphyromonas gingivalis, Streptococcus mutans inhibiting effect.And synchronous setting control 1, control 1 is according to tradition side
Biological lysozyme is directly added into mouthwash by method, measurement result such as table 4.
Table 4
Note: application examples 1~6 and the content of the biological lysozyme in control 1 are identical in table 4.
Application examples 3
7~14 corresponding compositions of additives containing biological lysozyme will be formulated in 1 table 1 of embodiment to be added to gargling
In water.The formula of each mouthwash is shown in Table the application examples 1~8 in 5.
Referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, the measurement correspondence of application examples 1~8 is gargled
The lysozyme activity of water.10 times of deionized water of mouthwash is diluted, centrifuging and taking supernatant dilutes 500 with deionized water later
It is detected after times.
Referring to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB 15979-2002C4
Stripping property resists (suppression) bacterium product bacteriostasis property test method, measures the mouthwash of application examples 1~8 to Escherichia coli, golden yellow Portugal
Grape coccus, porphyromonas gingivalis, Streptococcus mutans inhibiting effect.And synchronous setting control 1, control 1 is according to tradition side
Biological lysozyme is directly added into mouthwash by method, measurement result such as table 5.
Table 5
Note: application examples 1~8 and the content of the biological lysozyme of control 1 are identical in table 5.
Application examples 4
1~6 corresponding compositions of additives containing biological lysozyme will be formulated in 1 table 1 of embodiment to be added to gargling
In water.The formula of each mouthwash is shown in Table the application examples 1~6 in 6.
Referring to the measuring method of lysozyme in GB/T 25879-2010 egg white, the measurement correspondence of application examples 1~6 is gargled
The lysozyme activity of water.10 times of deionized water of mouthwash is diluted, centrifuging and taking supernatant dilutes 500 with deionized water later
It is detected after times.
Referring to National Standard of the People's Republic of China's Disposable Sanitary Accessory sanitary standard GB 15979-2002C4
Stripping property resists (suppression) bacterium product bacteriostasis property test method, measures the mouthwash of application examples 1~6 to Escherichia coli, golden yellow Portugal
Grape coccus, porphyromonas gingivalis, Streptococcus mutans inhibiting effect.And synchronous setting control 1, control 1 is according to tradition side
Biological lysozyme is directly added into mouthwash by method, measurement result such as table 6.
Table 6
Note: application examples 1~6 and the content of the biological lysozyme in control 1 are identical in table 6.
The present invention is gargled by the biological lysozyme compositions of additives of addition special formulation in the mouthwash to different formulations
The activity of mouth organism in water lysozyme does not reduce, this illustrates that biological lysozyme compositions of additives can be added to any match
In the mouthwash of side, therefore, although the present invention is only illustrated with the mouthwash of four kinds of different formulations, this is not represented
Biological lysozyme compositions of additives can be only used in the mouthwash of above-mentioned four kinds of different formulations.
Claims (2)
1. a kind of mouthwash containing biological lysozyme, which is characterized in that the mouthwash contains each of following weight percent
Component: biological lysozyme compositions of additives 5%, moisturizer 1~30%, emulsifier 0.01~2%, sweetener 0.001~1% are fragrant
Essence 0.01~1%, coolant agent 0.001~0.2%；It is described biology lysozyme compositions of additives by following weight percent each group
It is grouped as: biological lysozyme 20%, locust bean gum or sodium alginate 0.1%, EDETATE SODIUM 0.2%, deionized water surplus.
2. mouthwash according to claim 1, which is characterized in that the mouthwash contains each group of following weight percent
Divide, biological lysozyme compositions of additives 5%, moisturizer 19~25%, emulsifier 1%, sweetener 0.05~1%, essence 0.2% is cool
Taste agent 0.05%.
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