CN107736489A - A kind of preparation method of composite piglet feed additive - Google Patents
A kind of preparation method of composite piglet feed additive Download PDFInfo
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- CN107736489A CN107736489A CN201711104646.4A CN201711104646A CN107736489A CN 107736489 A CN107736489 A CN 107736489A CN 201711104646 A CN201711104646 A CN 201711104646A CN 107736489 A CN107736489 A CN 107736489A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
Abstract
The invention discloses a kind of preparation method of composite piglet feed additive, belong to field of fodder.For the present invention by extracting the active component in pumpkin seeds, wherein main component can be converted into 5 hydroxytryptamines in suckling piglet body, can stimulate the factor for carrying pig feeding, maintain intestinal submucosa tissue structural integrity, and feeding makes cD4 in weanling pig blood+/ CD8+Ratio liter is resistance to, is added to the growth and development state that can improve jejunum stage casing intestinal villus in early-weaned pigletses diet, slows down the generation of diarrhoea.The present invention solves the laundering period after current weaned piglet because without breast milk fragrance and the problem of feed intake reduces, and edible feed for weaned can cause to suffer from diarrhoea, and ablactation stress causes immunity degradation.
Description
Technical field
The invention belongs to field of fodder, and in particular to a kind of preparation method of composite piglet feed additive.
Background technology
China is the first in the world pig big country, is the second in the world Feed Manufacturing big country, wherein pig feed accounts for the 40% of total amount
More than.Sucking pig Phase-feeding is the important stage that pig industry ensures the output value, and sucking pig wean simultaneously is puzzlement pig industry hair
One of subject matter of exhibition, and one of focus of sucking pig nutrient research.Pig-breeding is the important set of China's fowl poultry kind cultivation
Into part, and the quality of sucking pig quality, the speed of growth are directly connected to the marketing time of pig and go out meat amount.In recent years, with
The improvement of animal varieties and the progress of cultural technique, most of pig farms have used the breeding way of early weaning.Although early stage
Wean has the advantages that to promote to open food as early as possible, improves weight of weaning litter, reduces feedstuff-meat ratio, but because wean sucking pig is in strong growth
Stage of development, each histoorgan are also needed further to develop, and function is still needed further perfect, particularly digestive organs, is weaned in addition
The features such as sucking pig has fast, shortage congenital immunity of growing, and digestive function is immature, plus ablactation stress, causes to feed
Feed intake is not high, and digestibility reduces, slow-growing or even negative growth occur, easily diarrhoea, and the death rate is high, therefore to wean breast
The requirement of pig feed is very high.
At present the laundering period after weaned piglet because without breast milk fragrance and feed intake reduces, feed can cause the abdomen of piglet again
Rush down, gastric acid secretion deficiency after weaned piglet, pH value rise, pepsin, which is formed, to be reduced, and the digestibility of protein reduces, and is small intestine
The breeding of interior enteropathogenic E. Coli and other harmful pathogens provides favourable condition, and lactobacter growth is suppressed,
Cause to suffer from diarrhoea.Therefore, we are necessary to prepare a kind of feed addictive, solve the problems, such as nowadays to exist with this, have
Help the growth of sucking pig, promote the development of pig industry.
The content of the invention
The technical problems to be solved by the invention：Searched for food for the laundering period after current weaned piglet because without breast milk fragrance
The problem of amount reduces, and edible feed for weaned can cause to suffer from diarrhoea, and ablactation stress causes immunity degradation, there is provided a kind of compound piglet is raised
The preparation method of feed additives.
In order to solve the above technical problems, the present invention is using technical scheme as described below：
A kind of preparation method of composite piglet feed additive, it is characterised in that the preparation method comprises the following steps：
（1）Take anhydrous butter oil to add sterilized water, stirring, pasteurize, cooling, adjust pH to 6 ~ 7, add feeding grape glycosyloxy
Change enzyme and the mixing of feeding lipase, enzymolysis, dry, pulverize sieving, collect sieving particle, obtain enzymolysis product particle, it is standby；
（2）Take to crack rice and pulverize and sieve, collect sieving particle and add distilled water and Thermostable α-Amylase mixing, liquefy, cooling, then
Beta amylase and Pullulanase are added, saccharification, enzyme deactivation, filtering, takes filtrate, is cooled down, centrifugation, takes supernatant to be filtered by vacuum, is heated up,
Activated carbon stirring is added, is filtered, takes smoke filtrate first to cross resin cation and is swapped after resin anion (R.A.), 30 ~ 40 DEG C keep 1
~ 2h, rotary evaporation concentration, obtains concentrated extract, standby；
（3）Take pumpkin seeds to add hexane solution, ultrasound, filtering, take filter residue and drying, obtain pumpkin seeds skimmed milk, take pumpkin seeds to take off
Cosmetics is added in the sodium hydroxide solution that mass fraction is 60%, is hydrolyzed, cooling, by hydrolysate distillation water washing, is dried, is obtained
Dried extract, it is standby；
（4）Take and Eleutherococcus Extract root be dry, pulverize into sieving, collect sieving particle and add distilled water, be heated to 95 ~ 100 DEG C keep 1 ~
2h, centrifugation, takes supernatant A, rotary evaporation, is concentrated under reduced pressure, and takes concentrate to add the solution of trichloroacetic acid that mass fraction is 10% and stirs
Mix uniformly, place 18 ~ 24h, centrifugation, take supernatant B to add the ethanol solution that mass fraction is 80% and mix, centrifugation, it is heavy to take
Starch, washed, be freeze-dried with acetone, dialysis, obtain purified extract；
（5）According to the mass fraction, step is taken（1）50 ~ 80 parts of standby enzymolysis product particle, step（2）Standby concentration extraction
40 ~ 50 parts of thing, step（3）30 ~ 50 parts of standby dried extract, 20 ~ 25 parts of purified extract is well mixed, must compound piglet
Feed addictive.
The step（1）The mass ratio of middle anhydrous butter oil and sterilized water is 1：10；Adjust again in mass ratio 10 after pH：2：3
Add feeding glucose oxidase and feeding lipase；Enzymatic hydrolysis condition is：55 ~ 60 DEG C of 5 ~ 7h of enzymolysis；It crushed 100 mesh sieves.
The step（2）In crack rice and crushed 80 mesh sieves；The quality of sieving particle and distilled water and Thermostable α-Amylase
Than for 10：50：3；Liquefaction condition：90 ~ 95 DEG C of 10 ~ 15min of liquefying time；Again in mass ratio 9：1：3 add beta amylases and general
Shandong orchid enzyme；Saccharification condition：55 ~ 60 DEG C of 36 ~ 40h of saccharification；Enzyme deactivation condition：90 ~ 100 DEG C of 5 ~ 8min of enzyme deactivation；After heating in mass ratio
1：5 add activated carbon；Resins exchange condition：30 ~ 40 DEG C of 1 ~ 2h of holding.
The step（3）The mass ratio of middle pumpkin seeds and hexane solution is 1：20；Ultrasound condition：180 ~ 200w ultrasounds
1.5~2h；The mass ratio for the sodium hydroxide solution that pumpkin seeds skimmed milk is 60% with mass fraction is 1：8；Hydrolysising condition：105~
The h of vacuum hydrolysis 18 ~ 20 under the conditions of 110 DEG C.
The step（4）Middle Eleutherococcus Extract root dry, pulverize 100 mesh sieves in 50 ~ 55 DEG C；The matter of sieving particle and distilled water
Amount is than being 1：50, it is heated to 95 ~ 100 DEG C of 1 ~ 2h of holding；Mass ratio of the concentrate with mass fraction for 10% solution of trichloroacetic acid
For 1：1；The mass ratio for the ethanol solution that supernatant B is 80% with mass fraction is 1：3.
Compared with other method, advantageous effects are the present invention：
（1）The present invention prepares natural milk fragrance Feed flavours by the way of lipase enzymolysis, made an addition to using cream as base-material
The laundering period after weaned piglet can be shortened in pig starter feed, with avoid as far as possible piglet because without breast milk fragrance and feed intake is small asks
Topic, lipase enzymolysis product nonhazardous action component, milk taste flavouring agent can significantly improve feed intake after making an addition to feed, and milk is savory
Nutriment is abundant particularly contained by natural milk-taste flavouring agent can improve sucking pig nutrition intake for taste agent；
（2）The present invention is from middle extraction active material of cracking rice, and sweet taste is gentle, and tasty and refreshing no pleasant impression, mouthfeel is fabulous, piglet can be induced to enter
Food, increase pig weight, while maltitol is decomposed by the microorganisms in big enteral and declined for short-chain fat, intestinal pH, shows acid
Property, this can promote Calcium-ion absorption, increase bone amount and lifting bone strength performance, and it is steady to be provided simultaneously with moisture absorption, moisture releasing, be not easy to crystallize
Function, will not cause addition after feed it is hardened；
（3）For the present invention by extracting the active component in pumpkin seeds, wherein main component can be converted into 5- hydroxyls in suckling piglet body
Tryptamines, the factor for carrying pig feeding can be stimulated, maintain intestinal submucosa tissue structural integrity, feeding makes cD4 in weanling pig blood+/
CD8+Ratio liter is resistance to, is added to the growth and development state that can improve jejunum stage casing intestinal villus in early-weaned pigletses diet, subtracts
The generation of slow diarrhoea；
（4）The present invention extracts a kind of active polysaccharide from wilsonii root, can strengthen the invasion energy that body resists pathogenic microorganism
Power, polysaccharide component can promote piglet lymphopoiesis, improve the immunity of piglet body, adjust and exempt from caused by ablactation stress
Epidemic disease power declines；
（5）The present invention, by extracting milk fragrance material and sweet substance, it is first to add pig starter feed by cream and the extraction cracked rice
By attracting inducing action, attract the increase of piglet food-intake, then enter enteron aisle by the way that piglet is edible, intestine of young pigs is formed and protected
Shield, prevent diarrhoea from producing, promote Calcium-ion absorption, improve pigling immunity, feed after weaned piglet is played it is certain promote and
Protective effect.
Embodiment
Mass fraction is 80% anhydrous butter oil：New Zealand's import.
Feeding glucose oxidase and feeding lipase：It is purchased from Henan Ai Limu bio tech ltd.
Resin cation, resin anion (R.A.)：It is purchased from Langfang Ding Yuan chemical building materials Co., Ltd.
A kind of preparation method of composite piglet feed additive, comprises the following steps：
（1）Take anhydrous butter oil in mass ratio 1：10 add sterilized water, and 20 ~ 30min, 65 ~ 80 DEG C of pasteurizes are stirred in 50 ~ 60 DEG C
20min, 40 ~ 50 DEG C being cooled to, the sodium hydroxide solution for being 0.01mol/L with concentration adjusts pH to 6 ~ 7, then in mass ratio 10：
2：3 add feeding glucose oxidase and the mixing of feeding lipase, digest 5 ~ 7h at 55 ~ 60 DEG C, dry, pulverize 100 mesh sieves,
Sieving particle is collected, obtains enzymolysis product particle, it is standby；
（2）Take to crack rice and crushed 80 mesh sieves, collection sieving particle, in mass ratio 10：50：3 add distilled water and high temperature resistant α-shallow lake
Powder enzyme mixes, and liquefy 10 ~ 15min in 90 ~ 95 DEG C, is cooled to room temperature, then in mass ratio 9：1：3 add beta amylase and Propiram
Enzyme, be saccharified 36 ~ 40h, 90 ~ 100 DEG C of 5 ~ 8min of enzyme deactivation in 55 ~ 60 DEG C, filtering, takes filtrate, cools down, under the conditions of 6000r/min
10 ~ 15min is centrifuged, takes supernatant to be filtered by vacuum, is warming up to 80 ~ 85 DEG C, in mass ratio 1：5 addition activated carbons, stirring 30 ~
35min, filter, take smoke filtrate to take smoke filtrate first to cross resin cation and swapped after resin anion (R.A.), 30 ~ 40 DEG C of holdings 1 ~
2h, rotary evaporation concentrates under the conditions of 50 ~ 60 DEG C, obtains concentrated extract, standby；
（3）Take pumpkin seeds in mass ratio 1：20 add hexane solution, 180 ~ 200w, 1.5 ~ 2h of ultrasound, filtering, take filter residue and drying,
Pumpkin seeds skimmed milk is obtained, takes pumpkin seeds skimmed milk in mass ratio 1：8 add in the sodium hydroxide solution that mass fraction is 60%,
18 ~ 20h of vacuum hydrolysis, is cooled to room temperature under the conditions of 105 ~ 110 DEG C, by hydrolysate with water washing is distilled 2 ~ 3 times, dries, obtains dry
Dry extract, it is standby；
（4）Take Eleutherococcus Extract root to dry, pulverize 100 mesh sieves in 50 ~ 55 DEG C, collect sieving particle in mass ratio 1：50 add distillation
Water, 95 ~ 100 DEG C of 1 ~ 2h of holding are heated to, centrifugation, supernatant is taken, rotary evaporation, is concentrated under reduced pressure, take concentrate in mass ratio 1：1
The solution of trichloroacetic acid that mass fraction is 10% is added, 5 ~ 10 DEG C stir, and place 18 ~ 24h, centrifugation, take supernatant by quality
Than 1：3, which add the ethanol solution that mass fraction is 80%, mixes, and is heated to 50 ~ 60 DEG C of 20 ~ 30min of stirring, centrifuges, taking precipitate,
Washed with acetone, -4 DEG C of freeze-dryings, dialysis, obtain purified extract；
（5）According to the mass fraction, step is taken（1）50 ~ 80 parts of standby enzymolysis product particle, step（2）Standby concentration extraction
40 ~ 50 parts of thing, step（3）30 ~ 50 parts of standby dried extract, 20 ~ 25 parts of purified extract is well mixed, must compound piglet
Feed addictive.
Example 1
Mass fraction is 80% anhydrous butter oil：New Zealand's import.
Feeding glucose oxidase and feeding lipase：It is purchased from Henan Ai Limu bio tech ltd.
Resin cation, resin anion (R.A.)：It is purchased from Langfang Ding Yuan chemical building materials Co., Ltd.
A kind of preparation method of composite piglet feed additive, comprises the following steps：
（1）Take anhydrous butter oil in mass ratio 1：10 add sterilized water, and 20min, 65 DEG C of pasteurize 20min, drop are stirred in 50 DEG C
For temperature to 40 DEG C, the sodium hydroxide solution for being 0.01mol/L with concentration adjusts pH to 6, then in mass ratio 10：2：3 add feeding Portugal
Grape carbohydrate oxidase and the mixing of feeding lipase, digest 5h at 55 DEG C, dry, pulverize 100 mesh sieves, collect sieving particle, obtain enzyme
Product grain is solved, it is standby；
（2）Take to crack rice and crushed 80 mesh sieves, collection sieving particle, in mass ratio 10：50：3 add distilled water and high temperature resistant α-shallow lake
Powder enzyme mixes, and liquefy 10min in 90 DEG C, is cooled to room temperature, then in mass ratio 9：1：3 add beta amylase and Pullulanase, in
55 DEG C of saccharification 36h, 90 DEG C of enzyme deactivation 5min, filtering, take filtrate, cool down, 10min is centrifuged under the conditions of 6000r/min, takes supernatant
Vacuum filtration, is warming up to 80 DEG C, in mass ratio 1：5 add activated carbon, stir 30min, filter, take smoke filtrate to take smoke filtrate first mistake
Resin cation swaps after resin anion (R.A.), and 30 DEG C of holding 1h, rotary evaporation concentrates under the conditions of 50 DEG C, obtains concentration and carries
Thing is taken, it is standby；
（3）Take pumpkin seeds in mass ratio 1：20 add hexane solution, 180w ultrasound 1.5h, filtering, take filter residue and drying, obtain pumpkin
Sub- skimmed milk, take pumpkin seeds skimmed milk in mass ratio 1：8 add in the sodium hydroxide solution that mass fraction is 60%, in 105 DEG C of bars
Vacuum hydrolysis 18h under part, is cooled to room temperature, by hydrolysate with water washing is distilled 2 times, dries, obtains dried extract, standby；
（4）Take Eleutherococcus Extract root to dry, pulverize 100 mesh sieves in 50 DEG C, collect sieving particle in mass ratio 1：50 add distilled water,
95 DEG C of holding 1h are heated to, centrifugation, supernatant is taken, rotary evaporation, is concentrated under reduced pressure, take concentrate in mass ratio 1：1 adds quality
Fraction is 10% solution of trichloroacetic acid, and 5 DEG C stir, and places 18h, centrifugation, takes supernatant in mass ratio 1：3 add quality
The ethanol solution that fraction is 80% mixes, and is heated to 50 DEG C of stirring 20min, centrifugation, taking precipitate, is washed with acetone, -4 DEG C of freezings
Dry, dialysis, obtain purified extract；
（5）According to the mass fraction, step is taken（1）50 parts of standby enzymolysis product particle, step（2）Standby concentrated extract 40
Part, step（3）30 parts of standby dried extract, 20 parts of purified extract is well mixed, obtains compounding additive for feed for piglets.
Example 2
Mass fraction is 80% anhydrous butter oil：New Zealand's import.
Feeding glucose oxidase and feeding lipase：It is purchased from Henan Ai Limu bio tech ltd.
Resin cation, resin anion (R.A.)：It is purchased from Langfang Ding Yuan chemical building materials Co., Ltd.
A kind of preparation method of composite piglet feed additive, comprises the following steps：
（1）Take anhydrous butter oil in mass ratio 1：10 add sterilized water, and 30min, 80 DEG C of pasteurize 20min, drop are stirred in 60 DEG C
For temperature to 50 DEG C, the sodium hydroxide solution for being 0.01mol/L with concentration adjusts pH to 7, then in mass ratio 10：2：3 add feeding Portugal
Grape carbohydrate oxidase and the mixing of feeding lipase, digest 7h at 60 DEG C, dry, pulverize 100 mesh sieves, collect sieving particle, obtain enzyme
Product grain is solved, it is standby；
（2）Take to crack rice and crushed 80 mesh sieves, collection sieving particle, in mass ratio 10：50：3 add distilled water and high temperature resistant α-shallow lake
Powder enzyme mixes, and liquefy 15min in 95 DEG C, is cooled to room temperature, then in mass ratio 9：1：3 add beta amylase and Pullulanase, in
60 DEG C of saccharification 40h, 100 DEG C of enzyme deactivation 8min, filtering, take filtrate, cool down, 15min is centrifuged under the conditions of 6000r/min, takes supernatant
Liquid is filtered by vacuum, and is warming up to 85 DEG C, in mass ratio 1：5 add activated carbon, stir 35min, filter, and take smoke filtrate to take smoke filtrate first
Cross resin cation to swap after resin anion (R.A.), 40 DEG C of holding 2h, rotary evaporation concentrates under the conditions of 60 DEG C, must concentrate
Extract, it is standby；
（3）Take pumpkin seeds in mass ratio 1：20 add hexane solution, 200w ultrasound 2h, filtering, take filter residue and drying, obtain pumpkin seeds
Skimmed milk, take pumpkin seeds skimmed milk in mass ratio 1：8 add in the sodium hydroxide solution that mass fraction is 60%, in 110 DEG C of conditions
Lower vacuum hydrolysis 20h, is cooled to room temperature, by hydrolysate with water washing is distilled 3 times, dries, obtains dried extract, standby；
（4）Take Eleutherococcus Extract root to dry, pulverize 100 mesh sieves in 55 DEG C, collect sieving particle in mass ratio 1：50 add distilled water,
100 DEG C of holding 2h are heated to, centrifugation, supernatant is taken, rotary evaporation, is concentrated under reduced pressure, take concentrate in mass ratio 1：1 adds quality
Fraction is 10% solution of trichloroacetic acid, and 10 DEG C stir, and places 124h, centrifugation, takes supernatant in mass ratio 1：3 add matter
Measure the ethanol solution that fraction is 80% to mix, be heated to 60 DEG C of stirring 30min, centrifugation, taking precipitate, washed with acetone, -4 DEG C cold
It is lyophilized dry, dialysis, obtain purified extract；
（5）According to the mass fraction, step is taken（1）80 parts of standby enzymolysis product particle, step（2）Standby concentrated extract 50
Part, step（3）50 parts of standby dried extract, 25 parts of purified extract is well mixed, obtains compounding additive for feed for piglets.
Example 3
Mass fraction is 80% anhydrous butter oil：New Zealand's import.
Feeding glucose oxidase and feeding lipase：It is purchased from Henan Ai Limu bio tech ltd.
Resin cation, resin anion (R.A.)：It is purchased from Langfang Ding Yuan chemical building materials Co., Ltd.
A kind of preparation method of composite piglet feed additive, comprises the following steps：
（1）Take anhydrous butter oil in mass ratio 1：10 add sterilized water, and 25min, 73 DEG C of pasteurize 20min, drop are stirred in 55 DEG C
For temperature to 45 DEG C, the sodium hydroxide solution for being 0.01mol/L with concentration adjusts pH to 6.5, then in mass ratio 10：2：3 additions are feeding
Glucose oxidase and the mixing of feeding lipase, digest 6h at 57 DEG C, dry, pulverize 100 mesh sieves, collect sieving particle, obtain
Enzymolysis product particle, it is standby；
（2）Take to crack rice and crushed 80 mesh sieves, collection sieving particle, in mass ratio 10：50：3 add distilled water and high temperature resistant α-shallow lake
Powder enzyme mixes, and liquefy 12.5min in 93 DEG C, is cooled to room temperature, then in mass ratio 9：1：3 add beta amylase and Pullulanase,
Be saccharified 38h, 95 DEG C of enzyme deactivation 6.5min in 57 DEG C, filtering, takes filtrate, cools down, 12.5min is centrifuged under the conditions of 6000r/min, is taken
Supernatant is filtered by vacuum, and is warming up to 83 DEG C, in mass ratio 1：5 add activated carbon, stir 32.5min, filter, take smoke filtrate to take and take out
Filtrate is first crossed resin cation and swapped after resin anion (R.A.), and 35 DEG C of holding 1.5h, rotary evaporation is dense under the conditions of 55 DEG C
Contracting, obtains concentrated extract, standby；
（3）Take pumpkin seeds in mass ratio 1：20 add hexane solution, 190w ultrasound 1.75h, filtering, take filter residue and drying, obtain southern
Melon seeds skimmed milk, take pumpkin seeds skimmed milk in mass ratio 1：8 add in the sodium hydroxide solution that mass fraction is 60%, at 107 DEG C
Under the conditions of vacuum hydrolysis 19h, be cooled to room temperature, by hydrolysate distillation water washing 2 times, dry, obtain dried extract, it is standby；
（4）Take Eleutherococcus Extract root to dry, pulverize 100 mesh sieves in 53 DEG C, collect sieving particle in mass ratio 1：50 add distilled water,
98 DEG C of holding 1.5h are heated to, centrifugation, supernatant is taken, rotary evaporation, is concentrated under reduced pressure, take concentrate in mass ratio 1：1 adds matter
The solution of trichloroacetic acid that fraction is 10% is measured, 7 DEG C stir, and place 21h, centrifugation, take supernatant in mass ratio 1：3 add matter
Measure the ethanol solution that fraction is 80% to mix, be heated to 55 DEG C of stirring 25min, centrifugation, taking precipitate, washed with acetone, -4 DEG C cold
It is lyophilized dry, dialysis, obtain purified extract；
（5）According to the mass fraction, step is taken（1）65 parts of standby enzymolysis product particle, step（2）Standby concentrated extract 45
Part, step（3）40 parts of standby dried extract, 22.5 parts of purified extract is well mixed, obtains compounding additive for feed for piglets.
Reference examples：The feed addictive of Yichun City company production.
The additive of feed addictive obtained by examples detailed above and reference examples is separately added into equal pig starter feed, used
Feed and the feed of reference examples prepared by above-described embodiment carries out feeding piglet contrast test.Selective body heavy phase is near, 40 ages in days
Piggy 200, is randomly divided into control group, experimental group, carries out the contrast such as 100 daily gain, feedstuff-meat ratio and death rate by a definite date altogether
Experiment, experimental period, use conventional barrier, and every morning, each feeding in afternoon once, ensure that all pigs being capable of free choice feeding.Raised
Cheng Zhong, 6 points of every morning weigh to empty stomach pig, record data.By the experiment of 100 days by a definite date, as a result count such as
Table 1：
| Test event | Example 1 | Example 2 | Example 3 | Reference examples |
| Daily gain kg | 1.31 | 1.55 | 1.26 | 0.58~0.7 |
| Diarrhea rate % | 0 | 1 | 1 | 8~10 |
| Death rate % | 0 | 0 | 0 | 2~3 |
Summary, feed addictive of the invention add feed in it is more preferable to swine rearing effect, be worth of widely use.
Claims (5)
1. a kind of preparation method of composite piglet feed additive, it is characterised in that the preparation method comprises the following steps：
Take anhydrous butter oil to add sterilized water, stirring, pasteurize, cooling, adjust pH to 6 ~ 7, it is glycoxidative to add feeding grape
Enzyme and the mixing of feeding lipase, enzymolysis, dry, pulverize sieving, collect sieving particle, obtain enzymolysis product particle, standby；
Take to crack rice and pulverize and sieve, collect sieving particle and add distilled water and Thermostable α-Amylase mixing, liquefy, cooling, then add
Enter beta amylase and Pullulanase, be saccharified, enzyme deactivation, filtering, take filtrate, cool down, centrifugation, take supernatant to be filtered by vacuum, heat up, add
Enter activated carbon stirring, filter, take smoke filtrate first to cross resin cation and swapped after resin anion (R.A.), 30 ~ 40 DEG C of holdings 1 ~
2h, rotary evaporation concentration, obtains concentrated extract, standby；
Take pumpkin seeds to add hexane solution, ultrasound, filtering, take filter residue and drying, obtain pumpkin seeds skimmed milk, take pumpkin seeds skimmed milk
Add in the sodium hydroxide solution that mass fraction is 60%, hydrolyze, cooling, by hydrolysate distillation water washing, dry, obtain drying
Extract, it is standby；
Take Eleutherococcus Extract root to dry, pulverize sieving, collect sieving particle and add distilled water, be heated to 95 ~ 100 DEG C of 1 ~ 2h of holding, from
The heart, supernatant A is taken, rotary evaporation, is concentrated under reduced pressure, take concentrate to add the solution of trichloroacetic acid stirring that mass fraction is 10% equal
It is even, 18 ~ 24h is placed, centrifugation, takes supernatant B to add the ethanol solution that mass fraction is 80% and mixes, centrifugation, take precipitation
Thing, washed, be freeze-dried with acetone, dialysis, obtain purified extract；
According to the mass fraction, step is taken（1）50 ~ 80 parts of standby enzymolysis product particle, step（2）Standby concentrated extract 40
~ 50 parts, step（3）30 ~ 50 parts of standby dried extract, 20 ~ 25 parts of purified extract is well mixed, obtains compounding pig starter feed
Additive.
2. the preparation method of composite piglet feed additive according to claim 1, it is characterised in that the step（1）
The mass ratio of middle anhydrous butter oil and sterilized water is 1：10；Adjust again in mass ratio 10 after pH：2：3 add feeding glucose oxidase
With feeding lipase；Enzymatic hydrolysis condition is：55 ~ 60 DEG C of 5 ~ 7h of enzymolysis；It crushed 100 mesh sieves.
3. the preparation method of composite piglet feed additive according to claim 1, it is characterised in that the step（2）
In crack rice and crushed 80 mesh sieves；Sieving particle is 10 with the mass ratio of distilled water and Thermostable α-Amylase：50：3；Liquefy bar
Part：90 ~ 95 DEG C of 10 ~ 15min of liquefying time；Again in mass ratio 9：1：3 add beta amylase and Pullulanase；Saccharification condition：55~
60 DEG C of 36 ~ 40h of saccharification；Enzyme deactivation condition：90 ~ 100 DEG C of 5 ~ 8min of enzyme deactivation；In mass ratio 1 after heating：5 add activated carbon；Resin is handed over
Change condition：30 ~ 40 DEG C of 1 ~ 2h of holding.
4. the preparation method of composite piglet feed additive according to claim 1, it is characterised in that the step（3）
The mass ratio of middle pumpkin seeds and hexane solution is 1：20；Ultrasound condition：180 ~ 200w ultrasounds, 1.5 ~ 2h；Pumpkin seeds skimmed milk with
The mass ratio for the sodium hydroxide solution that mass fraction is 60% is 1：8；Hydrolysising condition：Vacuum hydrolysis 18 under the conditions of 105 ~ 110 DEG C ~
20 h。
5. the preparation method of composite piglet feed additive according to claim 1, it is characterised in that the step（4）
Middle Eleutherococcus Extract root dry, pulverize 100 mesh sieves in 50 ~ 55 DEG C；The mass ratio of sieving particle and distilled water is 1：50, it is heated to 95
~ 100 DEG C of 1 ~ 2h of holding；The mass ratio for the solution of trichloroacetic acid that concentrate is 10% with mass fraction is 1：1；Supernatant B and quality
The mass ratio for the ethanol solution that fraction is 80% is 1：3.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110137023A (en) * | 2010-06-16 | 2011-12-22 | 제주특별자치도(농업기술원) | Functional feed composition comprising acanthopanax koreanum nakai for pig breeding |
| CN105410395A (en) * | 2015-11-15 | 2016-03-23 | 珙县金翼牧业科技有限公司 | Non-antibiotics traditional Chinese medicine type piglet feed production method |
| CN107095000A (en) * | 2017-05-12 | 2017-08-29 | 麦仑(漳州)生物科技有限公司 | A kind of sweet tea milk flavoured type piglet feed flavouring agent and its preparation technology |
-
2017
- 2017-11-10 CN CN201711104646.4A patent/CN107736489A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110137023A (en) * | 2010-06-16 | 2011-12-22 | 제주특별자치도(농업기술원) | Functional feed composition comprising acanthopanax koreanum nakai for pig breeding |
| CN105410395A (en) * | 2015-11-15 | 2016-03-23 | 珙县金翼牧业科技有限公司 | Non-antibiotics traditional Chinese medicine type piglet feed production method |
| CN107095000A (en) * | 2017-05-12 | 2017-08-29 | 麦仑(漳州)生物科技有限公司 | A kind of sweet tea milk flavoured type piglet feed flavouring agent and its preparation technology |
Non-Patent Citations (4)
| Title |
|---|
| 何洋: ""天然奶香味饲料调味剂的研究"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 郭俊珍 等: ""碎米酶法制备高麦芽糖浆的工艺研究"", 《食品科学》 * |
| 韩杰: ""刺五加多糖对断奶仔猪免疫功能的调控作用及机理研究"", 《中国博士学位论文全文数据库 农业科技辑》 * |
| 马勤超: ""南瓜子中营养成分的分析研究"", 《中国优秀硕士学位论文 工程科技Ⅰ辑》 * |
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Application publication date: 20180227 |