CN107711287B - Method for producing black fungus by using liquid strain and quantitative inoculation device - Google Patents

Method for producing black fungus by using liquid strain and quantitative inoculation device Download PDF

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CN107711287B
CN107711287B CN201711013766.3A CN201711013766A CN107711287B CN 107711287 B CN107711287 B CN 107711287B CN 201711013766 A CN201711013766 A CN 201711013766A CN 107711287 B CN107711287 B CN 107711287B
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CN107711287A (en
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王恒
吴乃国
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Rizhao City Economic Crop Station
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00—Calcareous fertilisers
    • C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
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Abstract

The invention relates to the technical field of black fungus production, in particular to a method for producing black fungus by using liquid strains and a quantitative inoculation device. The device comprises a strain storage tank, wherein the strain storage tank is connected with a spray head through a pipeline, a flow pump and a flow metering valve are sequentially arranged between the strain storage tank and the spray head, and the flow pump and the flow metering valve are both connected with a controller; the front end of the spray head is an umbrella-shaped nozzle, and a plurality of through holes are formed in the nozzle; the rear end of the spray head is provided with a compressed air inlet which is connected with a compressed air storage tank through a pipeline, the compressed air storage tank is connected with an air compression pump, and an electromagnetic valve is arranged between the compressed air storage tank and the compressed air inlet and is connected with a controller; the spray head is provided with a switch, and the switch is connected with the controller. The method has the advantages of good effect of improving efficiency on the production of the black fungus, shortening production period, saving inoculation time, simple operation and good inoculation effect.

Description

Method for producing black fungus by using liquid strain and quantitative inoculation device
Technical Field
The invention relates to the technical field of black fungus production, in particular to a method for producing black fungus by using liquid strains and a quantitative inoculation device.
Background
Auricularia auricula, also called black vegetable, mulberry, indigowoad root and rhizome, tree chicken, wood moth and wood antler are named because the shape is similar to that of an ear, and the Auricularia auricula is obtained by adding the black brown color, is a plant of the family Auriculariaceae, has moderate and sweet property and human stomach and large intestine channels. Has effects in nourishing, moistening dryness, nourishing blood, benefiting stomach, promoting blood circulation, stopping bleeding, moistening lung, and moisturizing intestine.
Black fungus is a kind of edible fungus with rich nutrition, and is a traditional health food and export commodity in China. According to modern scientific analysis, each 100g of dried product of the black fungus contains 10.6 g of protein, 0.2 g of fat, 65 g of carbohydrate, 7 g of crude fiber, 375 mg of calcium, 201 mg of phosphorus and 185 mg of iron, and also contains 10.15 mg of vitamin B, 20.55 mg of vitamin B and 2.7 mg of nicotinic acid. Therefore, the black fungus is popular with the people all the time, is often used as an ingredient for cooking various famous dishes in China and the West, and has the functions of increasing appetite, nourishing and strengthening the body. The black fungus has certain adsorption capacity; has effects of cleaning stomach and intestine and digesting cellulose. Auricularia auricula can also be used as medicine.
The artificial cultivation mainly comprises cut-log and bag materials, the bag material cultivation is more and more trend, liquid strains are mostly adopted for inoculation, and in the process of producing black fungus by adopting the liquid strains, the most important is whether the formula of the used liquid culture medium is excellent or not, and whether the culture conditions are proper or not, the formula directly determines the growth of black fungus mycelium and the formation of metabolites, and in the bag material cultivation process, the formula composition of the bag materials influences the quality, the production efficiency and the growth cycle of the black fungus.
At present, the research on the interaction among all factors in the culture of the black fungus liquid strains is few, the raw material screening is not optimized, and the problems of low efficiency, high cost and long hypha culture period of the black fungus production method cannot be fundamentally solved.
On the other hand, the inoculation method is also diversified, the material surface inoculation method is the most commonly adopted method due to the ultralow pollution rate, the bacteria balls grow well under the condition of sufficient oxygen, but the inoculation gun is mostly adopted for some small-sized production users at present, the use amount of the bacteria is often not easy to control, the cost is high due to excessive use amount, the growth period is long due to excessive use amount, the yield is low, the uniformity is not high, and the production and the like are influenced slightly.
Disclosure of Invention
The invention aims at the problems and provides a method for producing black fungus by using liquid strains and a quantitative inoculation device, wherein the device comprises a strain storage tank, the strain storage tank is connected with a spray head through a pipeline, a flow pump and a flow metering valve are sequentially arranged between the strain storage tank and the spray head, and the flow pump and the flow metering valve are both connected with a controller; the front end of the spray head is an umbrella-shaped nozzle, and a plurality of through holes are formed in the nozzle; the rear end of the spray head is provided with a compressed air inlet which is connected with a compressed air storage tank through a pipeline, the compressed air storage tank is connected with an air compression pump, and an electromagnetic valve is arranged between the compressed air storage tank and the compressed air inlet and is connected with a controller; the spray head is provided with a switch, and the switch is connected with the controller. The method has the advantages of good effect of improving efficiency in black fungus production, production cycle shortening, inoculation time saving, simple operation and good inoculation effect.
The technical scheme of the invention is as follows:
a method for culturing Auricularia auricula with liquid strain bag comprises the following steps:
(1) Preparing a liquid strain: the culture medium of the liquid strain comprises the following components: 450-550g of lotus root starch, 1000-1300g of potato powder, 400-600g of red date powder, 30-70g of peptone, 200-350g of bran, 100g of glucose, 20g of magnesium sulfate, 100g of monopotassium phosphate, 10g of vitamin B and 100L of water; weighing the raw materials according to the composition ratio of the raw materials, adding the raw materials into a fermentation tank, sterilizing, cooling, and injecting 1000mL of black fungus rock-flask liquid mother seeds under an aseptic condition, wherein the inoculation amount is 2-3% by volume; the fermentation culture temperature is 24-26 deg.C, and the ventilation rate is 80-90m 3 H, fermenting for 7 days with the initial pH value of 7.0 to obtain black fungus liquid strains for later use;
(2) Preparing a black fungus culture medium, bagging and sterilizing: the black fungus culture medium is prepared from the following components in parts by weight: 50-60 parts of jujube sawdust, 10-20 parts of corncobs, 10-20 parts of cottonseed hulls, 5-10 parts of cotton straws, 10-20 parts of lotus leaf powder, 3-6 parts of sweet potato powder and 1.0-1.2 parts of quick lime; weighing the above raw materials in proportion, adding water, and stacking for 3-5 days to make water content at 50-60%; then bagging, forming a central cavity at one end, wherein the diameter of the cavity is 2-3cm, and the depth of the cavity is 3-5cm, and then sterilizing and cooling to obtain a black fungus culture bag material;
(3) Inoculating and culturing: opening one end of the bag material with a central cavity, adopting a quantitative inoculation device, placing a spray head of the inoculation device on the central cavity, starting a switch, spraying 8-12ml of liquid strains, uniformly spraying the liquid strains in a spray manner in the central cavity of the bag material, on the surface of the bag material and on the inner wall of the plastic bag close to the surface of the bag material, standing for 3-5 minutes, sealing the opening of the plastic bag, after the inoculation is finished, placing the bag into a sterilized culture chamber for spawn running culture, and during the culture period, keeping the temperature at 26-30 ℃, keeping the relative humidity at 60-70%, and growing hypha in the bag after 40-45 days to complete the hypha culture;
(4) Ear egress management
Indoor cultivation management is carried out on the bag material after hypha culture is finished, the temperature is controlled to be 20-24 ℃, and the growth time is 50-70 days.
The invention is also characterized in that:
in the step (3), the strains on the inner wall of the plastic bag permeate between the bag material and the wall of the plastic bag along the inner wall, and the inoculation area is large.
In the step (1), the liquid strain culture medium adopts lotus root starch, potato powder and red date powder as main raw materials which are matched with each other, the red date powder can provide a large amount of saccharides, and after components contained in the red date powder are compatible with black fungus, the effect of the black fungus is increased, and the edible quality is increased.
In the step (2), the jujube wood chips are waste branches and leaves of the pruned jujube trees, and are crushed into 100-120 meshes with fine granularity, so that the decomposition and absorption of strains are facilitated, and the growth efficiency is improved.
In the step (1), the culture medium of the liquid strain comprises the following components: 500g of lotus root starch, 1200g of potato powder, 500g of red date powder, 50g of peptone, 300g of bran, 100g of glucose, 20g of magnesium sulfate, 100g of monopotassium phosphate, 10g of vitamin B1 and 100L of water.
In the step (2), the black fungus culture medium is prepared from the following components in parts by weight: 55 parts of jujube sawdust, 15 parts of corncobs, 15 parts of cottonseed hulls, 8 parts of cotton straws, 20 parts of lotus leaf powder, 6 parts of sweet potato powder and 1.0 part of quick lime; weighing the above raw materials in proportion, adding water, and stacking for 3-5 days to make water content at 55%; then bagging, forming a central cavity at one end, wherein the diameter of the cavity is 2-3cm, and the depth of the cavity is 3-5cm, and then sterilizing and cooling to obtain the black fungus culture bag material.
The invention also provides a quantitative inoculation device which is characterized by comprising a strain storage tank, wherein the strain storage tank is connected with the spray head through a pipeline; the front end of the spray head is an umbrella-shaped nozzle, and a plurality of through holes are formed in the nozzle; the rear end of the spray head is provided with a compressed air inlet which is connected with a compressed air storage tank through a pipeline, the compressed air storage tank is connected with an air compression pump, and an electromagnetic valve is arranged between the compressed air storage tank and the compressed air inlet and is connected with a controller; the spray head is provided with a switch, and the switch is connected with the controller.
The quantitative inoculation device is characterized in that:
the spray head is also provided with a handle.
The switch setting on handle, when pressing the switch, controller control flow pump, flow metering valve, solenoid valve start simultaneously, when the flow reaches the setting value, controller control flow pump, flow metering valve, solenoid valve close simultaneously, the switch resets, when needs spray the bacterial next time, open the switch can, so the repeated work.
A baffle plate is arranged above the nozzle on the spray head, and the diameter of the baffle plate is 10-20cm.
The diameter of the nozzle is 3-5cm.
The invention has the beneficial effects that:
according to the bag culture method of the black fungus, the culture medium of the liquid strain and the bag culture medium are improved, and the lotus root starch (lotus leaf powder) and the red date powder (jujube wood chips) are added, so that homology of the two culture media is achieved, production of the strain is facilitated, the growth index of the strain is improved, and the growth period is shortened; on the other hand, the black fungus is matched with the red dates and the lotus roots, so that the quality of the black fungus is improved, the nutrient content is increased, and the medicinal value is increased.
In addition, the culture conditions of the liquid strains are properly adjusted, the bag culture conditions are properly adjusted, the culture time is shortened, the production efficiency is improved, and the income is increased.
Moreover, the structure of the bag material is adjusted, and the quantitative inoculation device is matched, so that the quantitative, rapid and uniform inoculation can be realized, the inoculation area is increased, the strain consumption is reduced, the strain growth speed is greatly increased, and the cost is reduced.
In a word, the invention provides a bag culture method and a quantitative inoculation device for black fungus, which have the advantages of good effect of improving the efficiency of the production of the black fungus, shortened production period, saved inoculation time, simple operation and good inoculation effect.
Drawings
FIG. 1 is a schematic view of the quantitative inoculating device of the present invention;
FIG. 2 is a cross-sectional view of the nozzle of FIG. 1;
fig. 3 is a schematic circuit diagram.
The device comprises a strain storage tank, a flow pump 2, a flow metering valve 3, an electromagnetic valve 4, an air compression pump 5, a compressed air storage tank 6, a nozzle 7, a through hole 71, a baffle 8, a spray head 9, a compressed air inlet 91, a handle 10, a switch 11 and a controller 13.
Detailed Description
The technical solution of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1
A method for culturing Auricularia auricula with liquid strain bag comprises the following steps:
(1) Preparing a liquid strain: the culture medium of the liquid strain comprises the following components: 500g of lotus root starch, 1200g of potato powder, 500g of red date powder, 50g of peptone, 300g of bran, 100g of glucose, 20g of magnesium sulfate, 100g of monopotassium phosphate, 10g of vitamin B and 100L of water.
Weighing the raw materials according to the raw material composition ratio, adding the raw materials into a fermentation tank, sterilizing, cooling, and injecting 1000mL of black fungus shake bottle liquid mother seeds under an aseptic condition, wherein the inoculation amount is 2% by volume; the fermentation culture temperature is 24 ℃, and the ventilation rate is 80m 3 H, fermenting for 7 days with the initial pH value of 7.0 to obtain black fungus liquid strains for later use;
(2) Preparing a black fungus culture medium, bagging and sterilizing: the black fungus culture medium is prepared from the following components in parts by weight: 55 parts of jujube sawdust, 15 parts of corncobs, 15 parts of cottonseed hulls, 8 parts of cotton straws, 20 parts of lotus leaf powder, 6 parts of sweet potato powder and 1.0 part of quick lime.
Weighing the above raw materials in proportion, adding water, mixing, and stacking for 4 days to make water content at 55%; then bagging, forming a central cavity at one end, wherein the diameter of the cavity is 3cm, and the depth of the cavity is 5cm, and then sterilizing and cooling to obtain a black fungus culture bag material;
(3) Inoculating and culturing: opening one end of the bag material with a central cavity, adopting a quantitative inoculation device, placing a spray head of the inoculation device on the central cavity, starting a switch, spraying 8-12ml of liquid strains, uniformly spraying the liquid strains in a spray manner in the central cavity of the bag material, on the surface of the bag material and on the inner wall of the plastic bag close to the surface of the bag material, standing for 3-5 minutes, allowing the strains on the inner wall of the plastic bag to penetrate between the bag material and the wall of the plastic bag along the inner wall, increasing the inoculation area, closing the opening of the plastic bag, completing inoculation, placing the bag in a sterile culture chamber for spawn running culture, keeping the temperature at 30 ℃, keeping the relative humidity at 65% during the culture period, and completing the hypha culture after hypha grows to fill the bag for 40 days;
(4) Ear egress management
And (4) cultivating and managing the bag material after mycelium culture in the room, controlling the temperature to be 20 ℃ and the growth time to be 65 days.
In the step (2), the jujube wood chips are waste branches and leaves of the pruned jujube trees, and are crushed into 100-120 meshes with fine granularity, so that the decomposition and absorption of strains are facilitated, and the growth efficiency is improved.
Example 2
In the step (1), the culture medium of the liquid strain comprises the following components: 450g of lotus root starch, 1000g of potato powder, 600g of red date powder, 70g of peptone, 200g of bran, 100g of glucose, 20g of magnesium sulfate, 100g of monopotassium phosphate, 10g of vitamin B1 and 100L of water.
Weighing the raw materials according to the raw material composition ratio, adding the raw materials into a fermentation tank, sterilizing, cooling, and injecting 1000mL of black fungus shake bottle liquid mother seeds under an aseptic condition, wherein the inoculation amount is 3% by volume; the fermentation culture temperature is 26 ℃, and the ventilation rate is 90m 3 And h, fermenting for 7 days at the initial pH value of 7.0 to obtain the black fungus liquid strain for later use.
The rest of the technical solution is given in example 1.
Example 3
In the step (1), the culture medium of the liquid strain comprises the following components: 550g of lotus root starch, 1300g of potato powder, 400g of red date powder, 30g of peptone, 350g of bran, 100g of glucose, 20g of magnesium sulfate, 100g of monopotassium phosphate, 10g of vitamin B and 100L of water.
Weighing the raw materials according to the composition ratio of the raw materials, adding the raw materials into a fermentation tank, sterilizing, cooling, and injecting 1000mL of black fungus rock-bottle liquid mother seeds under an aseptic condition, wherein the inoculation amount is 2% by volume; the fermentation culture temperature is 25 ℃, and the ventilation rate is 85m 3 H, fermenting for 7 days with the initial pH value of 7.0 to obtain black fungus liquid strains for later use;
the rest of the technical scheme is implemented by the embodiment 1.
Example 4
The black fungus culture medium is prepared from the following components in parts by weight: 50 parts of jujube sawdust, 20 parts of corncobs, 10 parts of cottonseed hulls, 10 parts of cotton straws, 20 parts of lotus leaf powder, 6 parts of sweet potato powder and 1.0 part of quick lime.
The rest of the technical scheme is implemented by the embodiment 1.
Example 5
The black fungus culture medium is prepared from the following components in parts by weight: 60 parts of jujube sawdust, 10 parts of corncobs, 20 parts of cottonseed hulls, 5 parts of cotton straws, 20 parts of lotus leaf powder, 6 parts of sweet potato powder and 1.0 part of quick lime.
The rest of the technical scheme is implemented by the embodiment 1.
Example 6
With reference to fig. 1-3, the invention also provides a quantitative inoculation device, which comprises a strain storage tank 1, wherein the strain storage tank 1 is connected with a spray head 9 through a pipeline, a flow pump 2 and a flow metering valve 3 are sequentially arranged between the strain storage tank 1 and the spray head 9, and the flow pump 2 and the flow metering valve 3 are both connected with a controller 13; the front end of the spray head is an umbrella-shaped nozzle 7, and a plurality of through holes 71 are arranged on the nozzle 7; a compressed air inlet 91 is arranged at the rear end of the spray head 9, the compressed air inlet 91 is connected with a compressed air storage tank 6 through a pipeline, the compressed air storage tank 6 is connected with an air compression pump 5, an electromagnetic valve 4 is arranged between the compressed air storage tank 6 and the compressed air inlet 91, and the electromagnetic valve 4 is connected with a controller 13; a switch 11 is arranged on the spray head 9, and the switch 11 is connected with a controller 13; a baffle plate 8 is arranged above the nozzle on the spray head 9, and the diameter of the baffle plate 8 is 10-20cm; the diameter of the nozzle 7 is 3-5cm and is adapted to the central hollow space of the bag material.
The spray head 9 is also provided with a handle 10, and a switch 11 is arranged on the handle 10. When the switch is pressed down, the controller 13 controls the flow pump 2, the flow metering valve 3 and the electromagnetic valve 4 to be started simultaneously, when the flow reaches a set value, the controller 13 controls the flow pump 2, the flow metering valve 3 and the electromagnetic valve 4 to be closed simultaneously, the switch 11 is reset, when the next spraying of the strains is needed, the switch 11 is opened, and the operation is repeated.

Claims (9)

1. A method for culturing Auricularia auricula with liquid strain bag comprises the following steps:
(1) Preparing a liquid strain: the culture medium of the liquid strain comprises the following components: 450-550g of lotus root starch, 1000-1300g of potato powder, 400-600g of red date powder, 30-70g of peptone, 200-350g of bran, 100g of glucose, 20g of magnesium sulfate, 100g of monopotassium phosphate, 110g of vitamin B and 100L of water; weighing the raw materials according to the raw material composition ratio, adding the raw materials into a fermentation tank, sterilizing, cooling, and injecting 1000mL of black fungus shake bottle liquid mother seeds under an aseptic condition, wherein the inoculation amount is 2-3% by volume percentage; the fermentation culture temperature is 24-26 deg.C, and the ventilation rate is 80-90m 3 H, fermenting for 7 days at the initial pH value of 7.0 to obtain black fungus liquid strains for later use;
(2) Preparing a black fungus culture medium, bagging and sterilizing: the black fungus culture medium is prepared from the following components in parts by weight: 50-60 parts of jujube sawdust, 10-20 parts of corncobs, 10-20 parts of cottonseed hulls, 5-10 parts of cotton straws, 10-20 parts of lotus leaf powder, 3-6 parts of sweet potato powder and 1.0-1.2 parts of quick lime; weighing the raw materials in proportion, adding water, preparing and stacking for 3-5 days to ensure that the water content is 50% -60%; then bagging, forming a central cavity at one end, wherein the diameter of the cavity is 2-3cm, and the depth of the cavity is 3-5cm, and then sterilizing and cooling to obtain a black fungus culture bag material;
(3) Inoculating and culturing: opening one end of the bag material with a central cavity, adopting a quantitative inoculation device, placing a spray head of the inoculation device on the central cavity, starting a switch, spraying 8-12mL of liquid strains, uniformly spraying the liquid strains in a spray manner in the central cavity of the bag material, on the surface of the bag material and on the inner wall of the plastic bag close to the surface of the bag material, standing for 3-5 minutes, sealing the opening of the plastic bag, after the inoculation is finished, placing the bag into a sterilized culture chamber for spawn running culture, and during the culture period, keeping the temperature at 26-30 ℃, keeping the relative humidity at 60% -70%, and growing hypha in the bag after 40-45 days to complete hypha culture;
(4) Ear egress management
Indoor cultivation management is carried out on the bag material after hypha culture is finished, the temperature is controlled to be 20-24 ℃, and the growth time is 50-70 days.
2. The method for cultivating Auricularia auricula-judae with liquid strain bags according to claim 1, wherein in the step (2), the jujube wood dust is the waste branches and leaves of the pruned jujube tree, and is crushed to 100-120 meshes.
3. The method for culturing Auricularia auricula with liquid strain bags according to claim 1, wherein the culture medium of the liquid strain in step (1) comprises: 500g of lotus root starch, 1200g of potato powder, 500g of red date powder, 50g of peptone, 300g of bran, 100g of glucose, 20g of magnesium sulfate, 100g of monopotassium phosphate, 110g of vitamin B and 100L of water.
4. The method for culturing Auricularia auricula with liquid strain bags according to claim 1, wherein in the step (2), the Auricularia auricula culture medium is prepared from the following components in parts by weight: 55 parts of jujube sawdust, 15 parts of corncobs, 15 parts of cottonseed hulls, 8 parts of cotton straws, 20 parts of lotus leaf powder, 6 parts of sweet potato powder and 1.0 part of quick lime; weighing the above raw materials in proportion, adding water, and stacking for 3-5 days to make water content at 55%; then bagging, forming a central cavity at one end, wherein the diameter of the cavity is 2-3cm, and the depth of the cavity is 3-5cm, and then sterilizing and cooling to obtain the black fungus culture bag material.
5. A quantitative inoculation device for bag-cultured black fungus by the method according to claim 1, which comprises a strain storage tank, wherein the strain storage tank is connected with a spray head through a pipeline, a flow pump and a flow metering valve are sequentially arranged between the strain storage tank and the spray head, and the flow pump and the flow metering valve are both connected with a controller; the front end of the spray head is an umbrella-shaped nozzle, and a plurality of through holes are formed in the nozzle; the rear end of the spray head is provided with a compressed air inlet which is connected with a compressed air storage tank through a pipeline, the compressed air storage tank is connected with an air compression pump, and an electromagnetic valve is arranged between the compressed air storage tank and the compressed air inlet and is connected with a controller; the spray head is provided with a switch, and the switch is connected with the controller.
6. The quantitative inoculation device of claim 5, wherein a handle is further provided on the spray head.
7. A dosing device as claimed in claim 5, characterized in that a baffle is provided on the spray head above the spray nozzle, the baffle having a diameter of 10-20cm.
8. A dosing device according to claim 5, wherein the nozzle has a diameter of 3-5cm.
9. The metered dose inoculating device of claim 5, wherein the switch is provided on the handle.
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