CN107669731B - 一种护肤组合物及其应用、保健品 - Google Patents
一种护肤组合物及其应用、保健品 Download PDFInfo
- Publication number
- CN107669731B CN107669731B CN201711163108.2A CN201711163108A CN107669731B CN 107669731 B CN107669731 B CN 107669731B CN 201711163108 A CN201711163108 A CN 201711163108A CN 107669731 B CN107669731 B CN 107669731B
- Authority
- CN
- China
- Prior art keywords
- powder
- parts
- concentrated
- skin care
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 62
- 230000036541 health Effects 0.000 title claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 74
- 240000007817 Olea europaea Species 0.000 claims abstract description 37
- 244000241257 Cucumis melo Species 0.000 claims abstract description 26
- 240000003394 Malpighia glabra Species 0.000 claims abstract description 25
- WVXRAFOPTSTNLL-NKWVEPMBSA-N 2',3'-dideoxyadenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO)O1 WVXRAFOPTSTNLL-NKWVEPMBSA-N 0.000 claims abstract description 24
- 235000014837 Malpighia glabra Nutrition 0.000 claims abstract description 24
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 23
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 claims abstract description 21
- 230000002087 whitening effect Effects 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 15
- 230000003712 anti-aging effect Effects 0.000 claims abstract description 10
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims abstract description 4
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims abstract description 4
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 claims description 18
- JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 claims description 17
- 235000012661 lycopene Nutrition 0.000 claims description 17
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 claims description 17
- 229960004999 lycopene Drugs 0.000 claims description 17
- 239000001751 lycopene Substances 0.000 claims description 17
- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 claims description 17
- 235000011399 aloe vera Nutrition 0.000 claims description 16
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 16
- 239000003826 tablet Substances 0.000 claims description 16
- 244000144927 Aloe barbadensis Species 0.000 claims description 15
- 235000002961 Aloe barbadensis Nutrition 0.000 claims description 15
- 235000002532 grape seed extract Nutrition 0.000 claims description 13
- 229940087603 grape seed extract Drugs 0.000 claims description 13
- 239000001717 vitis vinifera seed extract Substances 0.000 claims description 13
- 239000003814 drug Substances 0.000 claims description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 8
- 235000019359 magnesium stearate Nutrition 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000004375 Dextrin Substances 0.000 claims description 5
- 229920001353 Dextrin Polymers 0.000 claims description 5
- 235000019425 dextrin Nutrition 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000845 maltitol Substances 0.000 claims description 5
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 claims description 5
- 235000010449 maltitol Nutrition 0.000 claims description 5
- 229940035436 maltitol Drugs 0.000 claims description 5
- 235000019202 steviosides Nutrition 0.000 claims description 5
- SERLAGPUMNYUCK-DCUALPFSSA-N 1-O-alpha-D-glucopyranosyl-D-mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SERLAGPUMNYUCK-DCUALPFSSA-N 0.000 claims description 4
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 4
- 229920000881 Modified starch Polymers 0.000 claims description 4
- 239000000905 isomalt Substances 0.000 claims description 4
- 235000010439 isomalt Nutrition 0.000 claims description 4
- HPIGCVXMBGOWTF-UHFFFAOYSA-N isomaltol Natural products CC(=O)C=1OC=CC=1O HPIGCVXMBGOWTF-UHFFFAOYSA-N 0.000 claims description 4
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 4
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 4
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 4
- 239000000377 silicon dioxide Substances 0.000 claims description 4
- 235000012239 silicon dioxide Nutrition 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims description 2
- 239000002552 dosage form Substances 0.000 claims description 2
- 229940057948 magnesium stearate Drugs 0.000 claims description 2
- 239000006187 pill Substances 0.000 claims description 2
- 239000008176 lyophilized powder Substances 0.000 claims 2
- 239000000546 pharmaceutical excipient Substances 0.000 claims 2
- 239000004383 Steviol glycoside Substances 0.000 claims 1
- 235000019411 steviol glycoside Nutrition 0.000 claims 1
- 229930182488 steviol glycoside Natural products 0.000 claims 1
- 150000008144 steviol glycosides Chemical class 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 21
- 230000032683 aging Effects 0.000 abstract description 15
- 230000009758 senescence Effects 0.000 abstract description 7
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 230000008832 photodamage Effects 0.000 abstract description 4
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 abstract description 3
- 210000003491 skin Anatomy 0.000 description 57
- 239000000047 product Substances 0.000 description 23
- 238000002156 mixing Methods 0.000 description 15
- 150000003254 radicals Chemical class 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 13
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 12
- 241000700159 Rattus Species 0.000 description 11
- 235000009508 confectionery Nutrition 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 9
- 102000019197 Superoxide Dismutase Human genes 0.000 description 9
- 108010012715 Superoxide dismutase Proteins 0.000 description 9
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Natural products OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 9
- 229940118019 malondialdehyde Drugs 0.000 description 9
- 230000003078 antioxidant effect Effects 0.000 description 8
- 238000006206 glycosylation reaction Methods 0.000 description 8
- 230000002255 enzymatic effect Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 102000003425 Tyrosinase Human genes 0.000 description 6
- 108060008724 Tyrosinase Proteins 0.000 description 6
- 235000009754 Vitis X bourquina Nutrition 0.000 description 6
- 235000012333 Vitis X labruscana Nutrition 0.000 description 6
- 240000006365 Vitis vinifera Species 0.000 description 6
- 235000014787 Vitis vinifera Nutrition 0.000 description 6
- 206010048245 Yellow skin Diseases 0.000 description 6
- 239000003963 antioxidant agent Substances 0.000 description 6
- 239000011248 coating agent Substances 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- 230000013595 glycosylation Effects 0.000 description 6
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 5
- 108010024636 Glutathione Proteins 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 229930003268 Vitamin C Natural products 0.000 description 5
- 235000006708 antioxidants Nutrition 0.000 description 5
- 238000011049 filling Methods 0.000 description 5
- 229960003180 glutathione Drugs 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 235000019154 vitamin C Nutrition 0.000 description 5
- 239000011718 vitamin C Substances 0.000 description 5
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- JUUBCHWRXWPFFH-UHFFFAOYSA-N Hydroxytyrosol Chemical compound OCCC1=CC=C(O)C(O)=C1 JUUBCHWRXWPFFH-UHFFFAOYSA-N 0.000 description 4
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 description 4
- 229920001436 collagen Polymers 0.000 description 4
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- -1 lipid peroxide Chemical class 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 229940013618 stevioside Drugs 0.000 description 4
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 238000000576 coating method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 230000009759 skin aging Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 230000037303 wrinkles Effects 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000167854 Bourreria succulenta Species 0.000 description 2
- 102000006587 Glutathione peroxidase Human genes 0.000 description 2
- 108700016172 Glutathione peroxidases Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 241000779599 Malpighia Species 0.000 description 2
- 241000208949 Malpighiaceae Species 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 241000219094 Vitaceae Species 0.000 description 2
- 229930003427 Vitamin E Natural products 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 125000003172 aldehyde group Chemical group 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 150000001728 carbonyl compounds Chemical class 0.000 description 2
- 235000021466 carotenoid Nutrition 0.000 description 2
- 150000001747 carotenoids Chemical class 0.000 description 2
- 235000019693 cherries Nutrition 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000035617 depilation Effects 0.000 description 2
- 210000004207 dermis Anatomy 0.000 description 2
- 235000018927 edible plant Nutrition 0.000 description 2
- 230000007760 free radical scavenging Effects 0.000 description 2
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000005469 granulation Methods 0.000 description 2
- 230000003179 granulation Effects 0.000 description 2
- 235000021021 grapes Nutrition 0.000 description 2
- 210000004209 hair Anatomy 0.000 description 2
- 229940095066 hydroxytyrosol Drugs 0.000 description 2
- 235000003248 hydroxytyrosol Nutrition 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 125000000468 ketone group Chemical group 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000008055 phosphate buffer solution Substances 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000171 quenching effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- IKGXIBQEEMLURG-NVPNHPEKSA-N rutin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-NVPNHPEKSA-N 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 235000019165 vitamin E Nutrition 0.000 description 2
- 229940046009 vitamin E Drugs 0.000 description 2
- 239000011709 vitamin E Substances 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- JPFCOVZKLAXXOE-XBNSMERZSA-N (3r)-2-(3,5-dihydroxy-4-methoxyphenyl)-8-[(2r,3r,4r)-3,5,7-trihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2h-chromen-4-yl]-3,4-dihydro-2h-chromene-3,5,7-triol Chemical compound C1=C(O)C(OC)=C(O)C=C1C1[C@H](O)CC(C(O)=CC(O)=C2[C@H]3C4=C(O)C=C(O)C=C4O[C@@H]([C@@H]3O)C=3C=CC(O)=CC=3)=C2O1 JPFCOVZKLAXXOE-XBNSMERZSA-N 0.000 description 1
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 244000298697 Actinidia deliciosa Species 0.000 description 1
- 235000009436 Actinidia deliciosa Nutrition 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241001116389 Aloe Species 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 244000012254 Canarium album Species 0.000 description 1
- 235000009103 Canarium album Nutrition 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 240000000560 Citrus x paradisi Species 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 235000009844 Cucumis melo var conomon Nutrition 0.000 description 1
- 241000219104 Cucurbitaceae Species 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 1
- 206010033546 Pallor Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229920001991 Proanthocyanidin Polymers 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- XDSIUDMMHGFGHK-UHFFFAOYSA-N benzene-1,2-diol;ethanol Chemical compound CCO.OC1=CC=CC=C1O XDSIUDMMHGFGHK-UHFFFAOYSA-N 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 239000010796 biological waste Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000002951 depilatory effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 239000007938 effervescent tablet Substances 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940087559 grape seed Drugs 0.000 description 1
- 239000007902 hard capsule Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000007390 skin biopsy Methods 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 210000004003 subcutaneous fat Anatomy 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/364—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/48—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/01—Hydrocarbons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/32—Burseraceae (Frankincense family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Mycology (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Inorganic Chemistry (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明涉及保健品领域，特别涉及一种具有美白、抗衰老功效的护肤组合物及其保健品、用途。本发明提供的护肤组合物包括：浓缩香瓜粉、针叶樱桃粉和浓缩橄榄汁冻干粉。实验结果表明，本发明选用包括浓缩香瓜粉、针叶樱桃粉和浓缩橄榄汁冻干粉这三种天然药食同源的药材组成的组合物在美白、延缓衰老方面具有协同增效作用，能够有效地抑制酪氨酸形成、清除自由基、改善皮肤光损伤。以上结果表明，本发明提供的护肤组合物具有显著地美白、延缓衰老的作用。
Description
技术领域
本发明涉及保健品技术领域，具体涉及一种护肤组合物及其应用、保健品。
背景技术
现代医学对肤色暗黄生化本质的认识：关于皮肤衰老的机理研究得非常多，先后出现过很多学说，但能够较为合理地揭示肤色暗黄问题的主要有自由基衰老学说、非酶糖基化衰老学说。
肤色暗黄与自由基衰老学说：自由基(Free Radicals)衰老学说最早是由DenhamHarman于1955年提出来的。该学说认为：自由基具有极强的氧化能力，当机体内的自由基代谢处于不平衡状态时，过量的自由基就会引起机体损伤，将不饱和脂肪酸氧化成过氧化脂质(lipid peroxidase，LPO)，其终产物丙二醛(malonic dialdehyde，MDA)是很强的交联剂，与蛋白质、核酸等生物大分子交联，形成难溶性物质-脂褐素。人进入中老年之后，随着年龄的增大，皮肤内脂褐素类物质的量逐渐增多，从而使肤色暗黄现象愈发严重。
肤色暗黄与非酶糖基化衰老学说：非酶糖基化衰老学说(又称美拉德反应衰老学说)，作为分子水平的又一个重要的衰老学说，是近年来衰老机制研究的重点。生物体内非酶糖基化反应是指在无酶催化的条件下，还原性糖的醛基或酮基与蛋白质等大分子中的游离氨基反应生成可逆或不可逆结合物-高级糖基化终末产物(Advanced glycation end-products,AGEs)的过程。Dyer等发现皮肤衰老与非酶糖基化有关，皮肤的真皮层中富含胶原蛋白和弹性蛋白，蛋白质分子中的氨基酸容易与细胞外液中葡萄糖的醛基或酮基发生非酶糖基化反应，且随着年龄的增长，AGEs进行性增加，AGEs发生褐变后形成棕黄色的生物垃圾，堆积在皮肤的真皮层，令肌肤失去剔透感而泛黄、暗沉。此外，皮肤中AGEs增加，使胶原蛋白形成分子间交联，不但降低了皮肤的弹性和通透性，使养料及废物的扩散性能减弱，而且降低了胶原的可溶性使其难以被胶原酶水解，造成皮肤弹性下降，皱纹不易平复且不断加深，从而加快皮肤的衰老过程。
现代医学解决肤色暗黄、延缓衰老问题主要从以上两方面入手：①抑制非酶糖基化反应，消除羰基化合物对肌肤的危害；②清除自由基、避免脂质过氧化，减少羰基化合物的产生和肤色暗黄的形成。
目前市场上具有美白、抗衰老功效的保健品种类繁多、品质参差不齐，能够有效解决肤色暗黄、延缓衰老问题，且效果显著的保健品并不多见。
发明内容
本发明的目的在于提供一种具有美白、延缓衰老功效的护肤组合物及其应用、保健品。
为实现上述发明目的，本发明提供如下技术方案：
本发明提供的护肤组合物，包括浓缩香瓜粉、针叶樱桃粉和浓缩橄榄汁冻干粉。
作为优选，以重量份计，所述护肤组合物包括如下组分：
浓缩香瓜粉10-50份、针叶樱桃粉10-40份和浓缩橄榄汁冻干粉1-15份。
作为优选，所述组合物还包括库拉索芦荟凝胶、葡萄籽提取物和番茄红素。
作为优选，以重量份计，所述护肤组合物包括如下组分：
浓缩香瓜粉10-50份、针叶樱桃粉10-40份、浓缩橄榄汁冻干粉1-15份、库拉索芦荟凝胶1-10份、葡萄籽提取物1-5份和番茄红素0.1-1份。
本发明选用浓缩香瓜粉、针叶樱桃粉和浓缩橄榄汁冻干粉这三种天然药食两用植物提取物组成的组合物在美白、延缓衰老方面表现出协同增效作用，能够有效地抑制酪氨酸形成、清除自由基、改善皮肤光损伤，表明本发明所述组合物美白、延缓衰老的效果显著。在上述几味药材的基础上，添加库拉索芦荟凝胶、葡萄籽提取物和番茄红素，能够更显著的增强美白、延缓衰老的作用。
香瓜又称甘瓜或甜瓜，葫芦科植物的果实肉质，具多室及多种子，和浆果很像。香瓜中抗氧化性物质含量极高，以SOD和维生素C为主，能起到清除体内自由基，缓解衰老，也有一定的抗紫外线的功效，降低色素的形成和沉淀，能起到很好的美容养颜功效。尤其是在阳光充足的季节,能够避免色素沉着,起到防晒、增白、抗皱之保护皮肤的作用。
橄榄(Canarium album(Lour.)Raeusch.)又名山榄，又称谏果，橄榄科橄榄属乔木植物。南京人称它为青果，岭南人称作橄榄，不论生啖或是煮汁，它都能生津止渴、治咽喉瘸、解鱼熬之毒和喉闻骨鲠。橄榄营养丰富，果肉内含蛋白质、碳水化合物、脂肪、维生素C以及钙、磷、铁等矿物质。用现代生物技术分析，橄榄中主要活性物质为多酚类化学成，研究证明橄榄多酚具有较强的自由基清除能力及较高的抗氧化活性，橄榄多酚的自由基清除能力高于人工合成抗氧化剂BHA和抗坏血酸。羟基酪醇(hydroxytyrosol)，又名3，4-二羟基苯乙醇，是一种天然抗氧化剂，广泛存在于橄榄(olive)科橄榄属植物的枝叶及果实中，特别是地中海地区的橄榄树中含量较高，是橄榄油的有效成分。大量的研究表明羟基酪醇具有很强的抗氧化作用(Aeschbach et al.1994；Visioli et al.1998)，可阻止多不饱和脂肪酸的自氧化(Tsinidou et al.1995)。羟基酪醇对自由基的清除能力比其他一些合成的和天然的抗氧剂的抗氧化能力都高，例如，它是维生素E抗氧化性的10倍、二丁基羟基甲苯(BHT)的1000多倍。Goya等研究发现羟基酪醇能够降低由叔丁基过氧化氢所引起HepG2细胞内的ROS水平和脂质过氧化水平增高，阻止了叔丁基过氧化氢所导致的谷胱甘肽下降。谷胱甘肽为细胞所分泌的内生性主要抗氧化物质，能直接中和自由基和活性氧分子。
针叶樱桃，金虎尾科(Malpighiaceae)金虎尾属(Malpighia)凹缘金虎尾(M.emarginata)的果实。主产于巴西，其维生素C、维生素P(芦丁)、超氧化歧化酶(SOD)含量均雄冠世界所有水果，有“三王之果”之称。特别是维生素C，100克鲜果维生素C含量3500毫克，相当于苹果的500倍，柑橘的100倍，葡萄的600倍，梨的200倍，猕猴桃的12倍，被誉为维C之王。
库拉索芦荟凝胶，以库拉索芦荟叶片为原料，经沥醌清洗、去皮、漂烫、杀菌等步骤制成，芦荟凝胶冻干粉在滋润干燥肌肤、祛斑除痘、削除皱纹、恢复皮肤弹性，提高人体免疫力，清除自由基，缓解发炎和受损皮肤的疼痛等方面有着独特神奇的功效。
葡萄籽，葡萄籽是葡萄的种子，是葡萄酒和其他产品的下脚料，经晒干后分离葡萄皮、葡萄梗后所得产物，占整粒葡萄质量的4％～6％。葡萄籽含有丰富的氨基酸、维生素、矿物质和膳食纤维等多种营养物物质以及多种活性成分，如儿茶素类和原花青素类等多酚类物质，具有保健、美容之功效。
番茄红素，是类胡萝卜素的一种,主要存在于番茄、西瓜、葡萄柚和木瓜等食品中。番茄红素是从番茄中提取的天然生物抗氧化剂,有较独特的长链分子结构。研究表明番茄红素的抗氧化能力是维生素E的1 0 0倍。一个番茄红素分子可以清除数千个单线态氧。番茄红素是淬灭单线态氧和清除过氧化氢、自由基最强的类胡萝卜素,抑制、破坏单线态氧能力是胡萝卜素的2倍。因此番茄红素可以通过淬灭单线态氧预防脂类氧化,保护生物膜免受自由基的伤害,达到保护容颜,延缓衰老的作用。
在本发明提供的一些具体实施例中，以重量份计，所述护肤组合物包括如下组分：
浓缩香瓜粉30份、针叶樱桃粉20份、浓缩橄榄汁冻干粉15份、库拉索芦荟凝胶4份、葡萄籽提取物1份和番茄红素0.1份。
在本发明提供的一些具体实施例中，以重量份计，所述护肤组合物包括如下组分：
浓缩香瓜粉50份、针叶樱桃粉10份、浓缩橄榄汁冻干粉1份、库拉索芦荟凝胶4份、葡萄籽提取物1份和番茄红素0.1份。
在本发明提供的一些具体实施例中，以重量份计，所述护肤组合物包括如下组分：
浓缩香瓜粉10份、针叶樱桃粉40份、浓缩橄榄汁冻干粉12份、库拉索芦荟凝胶4份、葡萄籽提取物1份和番茄红素0.1份。
在本发明提供的一些具体实施例中，以重量份计，所述护肤组合物包括如下组分：
在本发明提供的一些具体实施例中，以重量份计，所述护肤组合物包括如下组分：
浓缩香瓜粉50份、针叶樱桃粉10份、浓缩橄榄汁冻干粉1份。
本发明提供的护肤组合物中，浓缩香瓜粉、针叶樱桃粉、浓缩橄榄汁冻干粉、库拉索芦荟凝胶和葡萄籽提取物可以是冻干粉，也可以是浓缩物，所述浓缩物和所述冻干粉的功效量应与所述原提取物成分份量相等。
本发明还提供了所述护肤组合物在制备具有美白、抗衰老功效的药物或保健品中的应用。
本发明还提供了一种具有美白抗衰老作用的药物，包括所述护肤组合物和药学上可接受的辅料。
本发明还提供了一种具有美白、抗衰老作用的保健品，包括所述护肤组合物和保健品中可接受的辅料。
作为优选，所述保健品中可接受的辅料选自异麦芽酮糖醇、微晶纤维素、硬脂酸镁、麦芽糖醇、抗性糊精、甜菊糖苷、预胶化淀粉、二氧化硅中的一种或两种以上。
在本发明提供的一些具体实施例中，以重量份计，所述保健品中可接受的辅料为异麦芽酮糖醇20-40份、微晶纤维素20-40份、硬脂酸镁1-5份。
在本发明提供的一些具体实施例中，以重量份计，所述保健品中可接受的辅料为麦芽糖醇20-40份、抗性糊精20-40份、甜菊糖苷1-5份。
在本发明提供的一些具体实施例中，以重量份计，所述保健品中可接受的辅料为预胶化淀粉20-40份、硬脂酸镁0.5-2份、二氧化硅0.5-2份。
本发明中，保健食品的剂型为片剂、胶囊剂、丸剂、颗粒剂、粉剂剂、口服液剂。
作为优选，所述保健品为压片糖果。压片糖果是粉末、颗粒经制粒或干粉混合(无需制粒)直接压片而成的产品，主要类型有普通压片糖、包衣压片糖、多层压片糖、泡腾压片糖以及咀嚼型压片糖。包装时尚、方便的压片糖果以其产品口味丰富、具清新口气或保健功能等特点，成为近年来糖果市场发展最快的糖果品类之一。
实验结果表明，本发明选用浓缩香瓜粉、针叶樱桃粉和浓缩橄榄汁冻干粉这三种天然药食两用植物提取物组成的组合物在美白、延缓衰老方面表现出协同增效作用，能够有效地抑制酪氨酸形成、清除自由基、改善皮肤光损伤。在上述几味药材的基础上，添加库拉索芦荟凝胶、葡萄籽提取物和番茄红素后能够明显的增强美白、延缓衰老的作用。
具体实施方式
本发明公开了一种护肤组合物及其应用、保健品，本领域技术人员可以借鉴本文内容，适当改进工艺参数实现。特别需要指出的是，所有类似的替换和改动对本领域技术人员来说是显而易见的，它们都被视为包括在本发明。本发明的方法及应用已经通过较佳实施例进行了描述，相关人员明显能在不脱离本发明内容、精神和范围内对本文所述的方法和应用进行改动或适当变更与组合，来实现和应用本发明技术。
对所公开的实施例的说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。
下面结合实施例，进一步阐述本发明：
实施例1本发明护肤组合物
浓缩香瓜粉10份、针叶樱桃粉40份、浓缩橄榄汁冻干粉12份，混合，即得。
实施例2本发明护肤组合物
浓缩香瓜粉50份、针叶樱桃粉10份、浓缩橄榄汁冻干粉1份，混合即得。
实施例3本发明护肤组合物
浓缩香瓜粉30份、针叶樱桃粉20份、浓缩橄榄汁冻干粉15份，混合即得。
实施例4本发明护肤组合物
浓缩香瓜粉30份、针叶樱桃粉20份、浓缩橄榄汁冻干粉15份、库拉索芦荟凝胶4份、葡萄籽提取物1份、番茄红素0.1份，混合即得。
实施例5本发明护肤组合物
浓缩香瓜粉50份、针叶樱桃粉10份、浓缩橄榄汁冻干粉1份、库拉索芦荟凝胶4份、葡萄籽提取物1份和番茄红素0.1份，混合即得。
实施例6本发明护肤组合物
浓缩香瓜粉10份、针叶樱桃粉40份、浓缩橄榄汁冻干粉12份、库拉索芦荟凝胶4份、葡萄籽提取物1份和番茄红素0.1份，混合即得。
比较例1～6的护肤组合物
比较例1～6的护肤组合物，如表1所示。
表1比较例1～6的护肤组合物
实施例7体外试验
将实施例1～6和比较例1～6的组合物，加水稀释，得到终浓度为10mg/mL的样品液。
(1)抑制酪氨酸酶活性的测定：在各试管中分别加入磷酸盐缓冲液(PBS)和样品液、酪氨酸酶溶液，混合均匀，于恒温水浴(37℃)反应10min，分别迅速移入多巴溶液1mL，混匀，放回水浴中反应5min，当反应至5min时马上测各收拾样品在475nm处的吸光度，计算供试药液对酪氨酸酶的抑制率。
(2)DPPH自由基清除率的测定：精密移取DPPH自由基溶液、样品液和无水乙醇各2mL于同一试管中，混匀后避光静置20min，分别测定517nm处的吸光度，计算供试药液对DPPH的清除率。
结果如表2所示。
表2不同配方酪氨酸酶抑制率、DPPH清除率结果
注：综合评分＝酪氨酸酶抑制率×50％+DPPH清除率×50％。
根据表2数据可知，实施例1～6组对酪氨酸酶抑制率及DPPH清除率均明显优于单药各组和比较例1～6组，具有显著的美白作用。
实施例8本发明组合物对大鼠皮肤光损伤的防护作用
动物分组：取Wistar大鼠120只，5周龄，体重122.5±5.5g，雌雄各半，按清洁动物标准条件雌雄分笼喂养。随机分为15组，包括正常对照组、模型对照组、单药组和试验组，每组8只，按照表3所示的给药方式灌胃给药(0.83g/kg)，连续给药4周，试验期间大鼠正常饮水，正常饲喂普通基础颗粒饲料。
表3分组与给药方式
实验方法：
动物脱毛+UVA照射：将大鼠用乙醚吸入法适度麻醉，以眼科剪刀剪短背部鼠毛至1mm，然后用电动剃须刀将剩余鼠毛剃净，脱毛区约5.0×4.5cm。照射前6h脱毛。
标本取材：照射后24h乙醚吸入法麻醉大鼠，以皮肤活检术取下背部脱毛区中央处皮肤，尽量去除皮下组织，生理盐水洗净后分别置入10％中性福尔马林和液氮中保存备用。标本取材后，常规10％福尔马林固定，石蜡包埋4μm连续切片，常规HE染色。
皮肤MDA含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性的检测：末次UVA十UVB照射实验结束后，处死大鼠，取大鼠背部照射区部分皮肤，去除皮下脂肪称重，制成10％组织匀浆，以3000转/分钟的速度离心10分钟，取上清液进行各指标测定。
结果如表4所示。
表4丙二醛(MDA)含量及抗氧化酶活性测定(n＝8，x±s)
| 组别 | MDA(nmol/mg prot) | SOD(U/mg port) | GSH-Px(U/mg port) |
| 对照组 | 13.76±1.11 | 14.28±1.48 | 12.13±1.83 |
| UVA+UVB照射组 | 25.05±1.64<sup>▲▲</sup> | 8.75±1.29<sup>▲▲</sup> | 7.88±1.10<sup>▲▲</sup> |
| UVA+UVB照射+单药组 | |||
| 浓缩香瓜粉 | 24.21±1.11 | 8.30±1.49 | 8.50±1.08 |
| 针叶樱桃粉 | 24.50±1.18 | 8.71±0.78 | 8.88±1.10 |
| 浓缩橄榄汁冻干粉 | 24.98±1.15 | 8.82±1.08 | 8.90±1.29 |
| UVA+UVB照射+试验组 | |||
| 实施例1组 | 23.41±0.78* | 10.38±1.45* | 9.20±1.49* |
| 实施例2组 | 18.10±1.10** | 10.04±1.12* | 9.77±1.83* |
| 实施例3组 | 22.77±1.95* | 9.94±1.09* | 9.70±1.95* |
| 实施例4组 | 14.30±1.25** | 15.76±1.28** | 14.98±1.25** |
| 实施例5组 | 15.10±0.88** | 14.24±1.08** | 13.51±1.87** |
| 实施例6组 | 15.91±1.06** | 13.44±1.56** | 12.08±1.37** |
| 比较例1组 | 24.01±0.83 | 9.77±1.86 | 9.02±1.45 |
| 比较例2组 | 24.15±1.45 | 8.97±1.46 | 8.96±1.41 |
| 比较例3组 | 23.98±1.00 | 9.43±1.44 | 9.03±1.76 |
| 比较例4组 | 23.85±1.04 | 9.61±1.11 | 8.99±1.38 |
| 比较例5组 | 23.99±1.08 | 9.66±0.83 | 9.09±1.47 |
| 比较例6组 | 24.15±1.19 | 9.56±1.02 | 9.01±1.44 |
注：与正常对照组比较：▲P＜0.05，▲▲P＜0.01；与模型对照组比较：*P＜0.05，**P＜0.01
结果显示，与对照组比较，UVA+UVB照射可使大鼠皮肤组织匀浆中MDA含量显著增高，SOD活性和谷胱甘肽过氧化物酶(GSH-Px)活性均显著降低。与UVA+UVB照射组比较，UVA+UVB照射+实施例1～6组能够显著降低皮肤组织匀浆中MDA的含量(P＜0.05)，显著提高SOD活性和GSH-Px活性(P＜0.05)。其中，实施例4的组合物对对紫外线防护效果最好。比较例1～6组的皮肤组织匀浆中MDA的含量、SOD活性和GSH-Px活性与模型组对比，未达显著差异，这表明，比较例组的组合物对对紫外线防护效果不明显。
实施例9美白抗衰功效测试试验
本实验使用荷兰DiagnOptics的AGE Reader(皮肤AGEs荧光光谱仪)，通过无创法测定受试者皮肤自体荧光(skin autofluorescence，AF)参考值判断实施例1是否可以降低皮肤组织中AGEs含量。入选受试者20人，女性，平均年龄28岁。受试期间口服实施例1的组合物，连续服用1个月。检测服用前后皮肤AGEs含量，结果如表5所示：
表5受试者服用前后肝功能变化比较(n＝20，x±s)
| 受试人数 | AF值 | |
| 服用前 | 20 | 1.8±0.3 |
| 服用后 | 20 | 1.5±0.2** |
注：服用前后对比，**P＜0.01
由表5结果可知，与服用前相比，受试者服用本发明实施例1的组合物后皮肤自体荧光值显著下降(P＜0.01)，表明本发明提供的组合物能够显著延缓皮肤衰老。
取实施例2～6所述的组合物进行美白抗衰功效测试试验，结果与实施例1相同或相近，无显著性差异(P>0.05)。
实施例10本发明护肤组合物片剂的制备
(1)称量、备料：称取实施例4所述组合物、异麦芽酮糖醇30份、微晶纤维素30份、硬脂酸镁3份，备用。
(2)混合：将上述除硬脂酸镁外的原料混合25min，然后加入硬脂酸镁再混合5min。
(3)压片：用压片机将上述混合料进行压片，0.5g/片，得到素片。
(4)包衣：称取配方量包衣剂，配置成适宜的固形物含量，在配桨锅中搅拌30～45分钟；搅拌完成后，对素片进行包衣，至片面基本成膜、颜色一致。
实施例11本发明护肤组合物粉剂的制备
(1)称量、称取实施例5所述组合物、麦芽糖醇30份、抗性糊精30份、甜菊糖苷3份，备用。
(2)混合：先将甜菊糖苷与抗性糊精混合10分钟，至颜色均匀后，再加入实施例5所述的组合物和麦芽糖醇，混合30分钟至完全均匀后出料。
(3)分装：按要求调整好设备，再将60mm空白铝箔膜准确安装于颗粒包装机上，待空机调试完毕后下料进行包装，至装量、外型符合要求才正式分装，净重量控制在每袋4克，每袋装量差异±4.5％，每10分钟检查一次装量差异，随时抽检外观和密封性。密封性用密封试验仪进行检测，真空度应小于或等于-60kpa，测试时间应该大于或等于3分钟。外袋上不得有粉粘结，不得有泄漏。
实施例12本发明护肤组合物硬胶囊的制备
(1)备料、混合：称取实施例6所述组合物、预胶化淀粉30份、硬脂酸镁1.5份、二氧化硅1.5份，备用。
(2)填充：混合好的物料在全自动填充剂上填充，装量0.5g/粒。
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。
Claims (8)
1.一种护肤组合物，其特征在于，
以重量份计，由如下组分组成：
浓缩香瓜粉10-50份、针叶樱桃粉10-40份和浓缩橄榄汁冻干粉1-15份。
2.一种护肤组合物，其特征在于，以重量份计，由下组分组成：
浓缩香瓜粉10-50份、针叶樱桃粉10-40份、浓缩橄榄汁冻干粉1-15份、库拉索芦荟凝胶1-10份、葡萄籽提取物1-5份和番茄红素0.1-1份。
3.根据权利要求2所述的护肤组合物，其特征在于，所述浓缩香瓜粉、所述针叶樱桃粉、所述浓缩橄榄汁冻干粉、所述库拉索芦荟凝胶、所述葡萄籽提取物为冻干粉或浓缩物。
4.权利要求1或2所述的护肤组合物在制备具有美白、抗衰老功效的药物或保健品中的应用。
5.一种具有美白抗衰老作用的药物，其特征在于，包括如权利要求1或2所述的组合物和药学上可接受的辅料。
6.一种具有美白、抗衰老作用的保健品，其特征在于，包括如权利要求1至5任一项所述的组合物和保健品中可接受的辅料。
7.根据权利要求6所述的保健品，其特征在于，所述保健品中可接受的辅料选自异麦芽酮糖醇、微晶纤维素、硬脂酸镁、麦芽糖醇、抗性糊精、甜菊糖苷、预胶化淀粉、二氧化硅中的一种或两种以上。
8.根据权利要求6所述的保健品，其特征在于，其剂型为片剂、胶囊剂、丸剂、颗粒剂、粉剂剂、口服液剂。
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711163108.2A CN107669731B (zh) | 2017-11-21 | 2017-11-21 | 一种护肤组合物及其应用、保健品 |
| PCT/CN2018/113403 WO2019100923A1 (zh) | 2017-11-21 | 2018-11-01 | 一种护肤组合物及其应用、保健品 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711163108.2A CN107669731B (zh) | 2017-11-21 | 2017-11-21 | 一种护肤组合物及其应用、保健品 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107669731A CN107669731A (zh) | 2018-02-09 |
| CN107669731B true CN107669731B (zh) | 2020-07-28 |
Family
ID=61149027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711163108.2A Active CN107669731B (zh) | 2017-11-21 | 2017-11-21 | 一种护肤组合物及其应用、保健品 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN107669731B (zh) |
| WO (1) | WO2019100923A1 (zh) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107669731B (zh) * | 2017-11-21 | 2020-07-28 | 汤臣倍健股份有限公司 | 一种护肤组合物及其应用、保健品 |
| CN113521136A (zh) * | 2021-08-04 | 2021-10-22 | 上海楷达生物科技有限公司 | 一种有助于皮肤美白和除皱的组合物 |
| CN114259515A (zh) * | 2021-12-31 | 2022-04-01 | 安琪纽特股份有限公司 | 一种具有抗糖化作用的组合物及应用 |
| CN114601006A (zh) * | 2022-01-28 | 2022-06-10 | 广州市诺品健康科技有限公司 | 一种美白压片糖果及其制备方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104382004A (zh) * | 2013-08-19 | 2015-03-04 | 宣城柏维力生物工程有限公司 | 一种针叶樱桃vc泡腾片 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105395458B (zh) * | 2015-12-15 | 2020-04-10 | 上海相宜本草化妆品股份有限公司 | 一种本草组合物及其应用 |
| CN106954813A (zh) * | 2017-03-16 | 2017-07-18 | 广州瓷肌化妆品有限公司 | 一种美白保健食品及其制备方法 |
| CN107669731B (zh) * | 2017-11-21 | 2020-07-28 | 汤臣倍健股份有限公司 | 一种护肤组合物及其应用、保健品 |
-
2017
- 2017-11-21 CN CN201711163108.2A patent/CN107669731B/zh active Active
-
2018
- 2018-11-01 WO PCT/CN2018/113403 patent/WO2019100923A1/zh active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104382004A (zh) * | 2013-08-19 | 2015-03-04 | 宣城柏维力生物工程有限公司 | 一种针叶樱桃vc泡腾片 |
Non-Patent Citations (1)
| Title |
|---|
| 西印度樱桃冻干粉提取液中Vc稳定性及美容功效初步研究;王领等;《香料香精化妆品》;20110831(第04期);29-32 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107669731A (zh) | 2018-02-09 |
| WO2019100923A1 (zh) | 2019-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107669731B (zh) | 一种护肤组合物及其应用、保健品 | |
| KR101776071B1 (ko) | 붉은 작두콩 추출물을 함유하는 항노화 및 미백용 조성물 | |
| KR102219594B1 (ko) | 죽순 껍질 추출물을 포함하는 화장료 조성물 | |
| EP1667699A1 (en) | Lotus and methyl donors | |
| CN112493480B (zh) | 一种抗氧化组合物及其应用 | |
| JP2003160505A (ja) | 体脂肪減少効果を有する飲食物及び外用組成物 | |
| JP6244594B2 (ja) | 老化抑制剤 | |
| KR101712608B1 (ko) | α-테르피네올을 유효성분으로 함유하는 피부 보습 및 피부 주름 개선용 조성물 | |
| KR101066676B1 (ko) | 경구용 피부 미용 개선용 조성물 | |
| JP2011055837A (ja) | 美容用飲食品 | |
| KR101721022B1 (ko) | 아디프산을 유효성분으로 함유하는 피부주름개선 및 피부탄력증진용 조성물 | |
| KR101111177B1 (ko) | 피부노화 방지용 생약재 추출물 및 이를 포함하는 화장료 조성물 | |
| JP4672269B2 (ja) | 抗老化剤、血小板凝集抑制剤、抗酸化剤、抗アレルギー剤、皮膚化粧料及び飲食品 | |
| JP2014105210A (ja) | 皮膚シワ生成の抑制及びシワ改善活性を有する白茶抽出物を含有する組成物 | |
| KR100937187B1 (ko) | 복분자주 제조 후 잔사로부터 기능성 화장품의 제조방법 | |
| KR20200006883A (ko) | 식물 추출물을 유효성분으로 포함하는 자외선에 의한 피부손상 예방 또는 개선용 조성물 | |
| KR101182059B1 (ko) | 항산화 활성을 갖는 톳 정유 추출물을 유효성분으로 함유하는 조성물 | |
| KR102154139B1 (ko) | 고로쇠나무 수액의 발효액, 카카오닙스 추출물 및 석류피 추출물을 포함하는 조성물 | |
| Marnewick | Antioxidant Properties of Rooibos (Aspalathus linearis)—In Vitro and in Vivo Evidence | |
| KR102212343B1 (ko) | 마리안 플럼 추출물을 유효성분으로 함유하는 피부 항노화 및 보습용 조성물 | |
| KR102032830B1 (ko) | 쌀겨 추출물을 함유하는 피부 개선용 건강기능식품 | |
| KR102573379B1 (ko) | 복합 생약 추출물을 포함하는 피부 개선용 조성물 | |
| JP3935360B2 (ja) | ヒアルロン酸産生促進剤並びに皮膚化粧料及び美容用飲食品 | |
| KR101940052B1 (ko) | 포제 추출물을 포함하는 조성물 | |
| KR102012170B1 (ko) | 바나나, 체리 및 호두의 혼합 추출물을 포함하는 피부 노화 방지 또는 개선용 조성물 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |