CN107668460A - A kind of selenium-enriched probioticses solid beverage and preparation method thereof - Google Patents
A kind of selenium-enriched probioticses solid beverage and preparation method thereof Download PDFInfo
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- CN107668460A CN107668460A CN201711026473.9A CN201711026473A CN107668460A CN 107668460 A CN107668460 A CN 107668460A CN 201711026473 A CN201711026473 A CN 201711026473A CN 107668460 A CN107668460 A CN 107668460A
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- selenium
- enriched
- probioticses
- cranberry
- solid beverage
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- 239000011669 selenium Substances 0.000 title claims abstract description 169
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 166
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 162
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 136
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- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 1
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- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 1
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 1
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- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
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- 125000003748 selenium group Chemical group *[Se]* 0.000 description 1
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/145—Gasseri
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Inorganic Chemistry (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of selenium-enriched probioticses solid beverage and preparation method thereof, belong to microbial food processing technique field.This selenium-enriched probiotics solid beverage is made up of selenium-enriched probioticses viable bacteria freeze-dried powder, microcapsules Cranberry extract, astragalus polyose, LBP-X, FOS；Its preparation method comprises the following steps：(1) Cranberry Feedstock treating；(2) prepared by Cranberry extract；(3) Cranberry extract microencapsulation and dry powder processed；(4) selenium-enriched probioticses culture；(5) selenium-enriched probioticses microencapsulation and dry powder processed；(6) prepared by polysaccharide；(7) each component compounds.This selenium-enriched probiotics solid beverage can adjust gut flora, improve immune system, and organic selenium content is high, can reach the effect of safe selenium-complement, and with the specific function protected female genitourinary system, safeguard vaginal health and skin health.
Description
Technical field
The invention belongs to microbial food processing technique field, and in particular to a kind of selenium-enriched probioticses solid beverage and its system
Preparation Method.
Background technology
Selenium (Se) is a kind of the essential trace elements of the human body, has extensive biological effect.Selenium has pole to life and health
Its important effect, selenium deficiency can cause a variety of diseases, such as Keshan disease and Kaschin-Beck disease.Selenium mainly have inorganic selenium and Organic Selenium and
Elemental selenium is several to be primarily present form, and Organic Selenium, elemental selenium, the bioavilability of inorganic selenium decline successively, and toxicity improves successively.
The source of inorganic selenium is wider, easily obtains, but inorganic selenium is less desirable as the absorption and utilization of selenium supplement hardening agent, its
Biological effectiveness is low, and toxicity is larger, and the scope between dosis toxica and requirement is small, thus is strictly limited its usage amount.It is prebiotic
Bacterium (such as lactic acid bacteria, lactobacillus, Bifidobacterium) can during the fermentation, by biotransformation by inorganic selenium or element
Selenium is converted into the selenium composition with more high bioavilability, such as selenocysteine, selenomethionine and the Guang ammonia of selenium methyl half
Acid.Thus, selenium-enriched probioticses are a kind of unique fermented products with extra nutritional value, can be used as human body supplement selenium element
Important selenium source.Modern medicine proves, selenium-enriched probioticses have regulation gut flora, antibacterial, anti-oxidant, anti-mutagenesis, anticancer,
Anti-inflammatory, anti-Kaschin-Beck disease, regulation fecundity, reducing blood lipid and other effects.
But inorganic selenium or elemental selenium are converted into the technology of Organic Selenium by biotransformation at present, conversion be present
The problem of rate is not high.Also there is the document that inorganic selenium is converted into Organic Selenium to record at present, it is special such as the patent No. 2006100972884
Sharp file, a kind of production method of the probiotic yogurt rich in selenoaminoacid is described, it is used adds in probiotic bacteria culture medium
Enter sodium selenite solution, be to utilize a certain amount of Bifidobacterium, Bulgarian Lactobacillus, lactobacillus acidophilus and streptococcus thermophilus
Inorganic selenium is converted into selenoaminoacid, but its conversion ratio is relatively low, only 50%, due to still suffered from finished product do not convert it is inorganic
Selenium, and content is higher, has certain toxicity, quality is difficult to be guaranteed.The and for example patent text of the patent No. 201310290985
Part, describes a kind of production method of the probiotic active milk beverage rich in Organic Selenium, and this method is first with saccharomyces cerevisiae by nothing
Machine selenium is converted into organism organic selenium, adds Bifidobacterium, Bulgarian Lactobacillus, lactobacillus acidophilus, thermophilus bacillus
And the probiotics such as bacillus cereus carries out secondary fermentation, inorganic selenium conversion ratio is up to 90%, but operating procedure step is more
It is more complicated.
Data are shown according to investigations, and crowd of the China with gynecological disease accounts for more than the 87% of female population at present, especially
The white collar women of one's late 30s, the people close to ninety percent have made a definite diagnosis gynecological disease.Gynecological disease makes women physiological health go from bad to worse, cloudy
Road inflammation, urethral infection, irregular menstruation and the face work and life that drastically influence women such as obscure.In traditional treatment
In scheme, treated for female reproductive health problem typically by antibiotic etc is taken, though effect is good, and it is secondary
Effect is big.Lack a product that is safe and reliable, convenient to take, can effectively safeguarding female reproductive health at present.
Cranberry (Cranberry), also known as mossberry, cranberry, european cranberry, it is a kind of higher fruit of nutritive value.
Cranberry has the characteristics of high-moisture, low in calories, high microsteping, more mineral matters, in its fruit rich in have vitamin C, OPC,
The composition such as phenolic acid and flavonols.Modern medicine is that preventing and treating women is daily it was demonstrated that Cranberry is a kind of natural antibacterial health fruit
The various bacterium infections of urinary system, urethritis, cystitis, chronic pyelonephritis, the optimal natural dietetic food of gastric ulcer；Together
When, Cranberry is also equipped with skin maintenance, anti-aging, anti-oxidant, anticancer, prevention of cardiovascular disease, protection oral cavity and dental health
Etc. a variety of specific functions.
The content of the invention
The invention aims to solve above mentioned problem existing for prior art, there is provided a kind of selenium-enriched probioticses solid drink
Product and preparation method thereof, not only sweet and sour taste, taste and color are all good for this selenium-enriched probiotics drink, and good health care effect, include
Selenium-enriched probioticses can adjust gut flora, improve immune system, its Organic Selenium high conversion rate, the existence of selenium essentially has
Machine selenium, the effect of safe selenium-complement is can reach, and cooperateed with Cranberry extract, complex polysaccharide, there is protection female urogenital
System, the specific function for safeguarding vaginal health and skin health.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of selenium-enriched probioticses solid beverage, it is 3 in mass ratio by compound selenium-enriched probioticses, complex polysaccharide, FOS：
1-1.2：1-1.2 composition；The compound selenium-enriched probioticses are extracted by selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry
Thing is 1 in mass ratio：4-5 is formed；It is 1 in mass ratio by astragalus polyose and LBP-X that the complex polysaccharide, which is,：1-1.5 group
Into.
Above-mentioned selenium-enriched probioticses drink, preferably, pressing matter by compound selenium-enriched probioticses, complex polysaccharide, FOS
Amount is than being 3：1：1 composition；The compound selenium-enriched probioticses are extracted by selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry
Thing is 1 in mass ratio：5 compositions；It is 1 in mass ratio by astragalus polyose and LBP-X that the complex polysaccharide, which is,：1 composition.
The preparation method of the selenium-enriched probioticses solid beverage, comprises the following steps：
(1) Cranberry Feedstock treating：By Cranberry fresh fruit cleaning removal of impurities after, crushed, collect Cranberry slurries and
Residue, it is well mixed, obtains Cranberry mixture；
(2) prepared by Cranberry extract：Cranberry mixture is sufficiently mixed with mass fraction for 75% ethanol solution,
Ultrasonic wave extraction is carried out again, and Extracting temperature is 45-50 DEG C, extraction time 3-5h, obtains Cranberry extract；
(3) Cranberry extract microencapsulation and dry powder processed：Cranberry extract obtained by step (2) is subjected to vacuum
Filtering, takes filtrate to be positioned over sterile ventilation 20-24h, then filtrate is prepared into microencapsulation form, obtains microcapsules Cranberry and carries
Take thing；
(4) selenium-enriched probioticses culture：Add Mixed Microbes in YEPD fluid nutrient mediums to be cultivated, Mixed Microbes are by plant breast
Bacillus, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus gasseri, lactobacillus acidophilus, streptococcus thermophilus composition, Mixed Microbes add
Add weight accounts for the 4-5% of YEPD fluid nutrient medium weight；In logarithmic phase addition early stage sodium selenite, sodium selenite addition concentration
For 10-15 μ g/ml, then carry out continuing to cultivate, cultivation temperature is 36-37 DEG C, incubation time 28-30h, it is prebiotic to obtain selenium-rich
The first nutrient solution of bacterium；By nutrient solution at the beginning of selenium-enriched probioticses and Bifidobacterium using weight ratio as 2-3：1 ratio mixing, obtains selenium-rich benefit
Raw bacteria culture fluid；
(5) selenium-enriched probioticses microencapsulation and dry powder processed：Selenium-enriched probioticses nutrient solution is subjected to ceramic membrane filter, obtained
Concentrated probiotics nutrient solution, by concentrated probiotics nutrient solution, the sodium alginate (microencapsulation) that mass concentration is 2%, maltose
Dextrin (cryoprotection) is sufficiently mixed, and is then freeze-dried, you can obtains selenium-enriched probioticses viable bacteria freeze-dried powder；
(6) prepared by polysaccharide：
A, prepared by astragalus polyose：The Radix Astragali is ground into Ultramicro-powder, addition cellulase digests to Radix Astragali Ultramicro-powder, enzyme
The solution time is 2-3h, and the addition weight of cellulase is the 0.6-0.8% of Radix Astragali Ultramicro-powder；It is yellow using the extraction of homogenate extraction method again
Astragalus polysaccharides, extraction twice, will filtering, concentration after extract solution mixes twice, astragalus polyose concentrate is obtained, with alcohol sedimentation by Huang
Astragalus polysaccharides concentrate is settled, separated, and precipitation is dried, you can obtain astragalus polyose；
B, prepared by LBP-X：After matrimony vine is ground into powder, the same astragalus polyose of other steps, you can it is more to obtain matrimony vine
Sugar；
(7) each component compounds：Selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract is fully mixed in proportion
Close, form compound selenium-enriched probioticses；Astragalus polyose and LBP-X are mixed in proportion, form complex polysaccharide；By compound selenium-rich
Probiotics, complex polysaccharide and FOS mix in proportion, you can obtain selenium-enriched probioticses solid beverage.
Further, in step (1), the Cranberry is that the method crushed with liquid nitrogen cryogenics is crushed, and is specially：
- 196 DEG C of liquid nitrogen are poured into Cranberry so that Cranberry quick freeze, be then placed in high speed disintegrator and crushed.
Further, in step (2), the weight ratio for the ethanol solution that Cranberry mixture is 75% with mass fraction is 1：
7-8, Extracting temperature are 48 DEG C, extraction time 4h.
Further, in step (3), the microcapsules Cranberry extract is to be extracted Cranberry using spray drying process
Thing filtrate is prepared into microencapsulation form, and concrete operations parameter is as follows：Wall material is maltodextrin, and core wall ratio is v：W=5：3, enter
170 DEG C, stream pressure 170KPa, feed rate 400mL/h of air temperature, 50 DEG C of feeding temperature.
Further, in step (4), the Mixed Microbes by etc. weight Lactobacillus plantarum, Lactobacillus rhamnosus, Luo Yishi
Lactobacillus, lactobacillus gasseri, lactobacillus acidophilus, streptococcus thermophilus composition.
Further, in step (5), the concentrated probiotics nutrient solution, the sodium alginate that mass concentration is 2%, malt
The mass ratio of magma essence is 5：3：2.
Further, in step (6), the extraction voltage of the homogenate extraction is the mass ratio of 80V, the Radix Astragali or matrimony vine and water
For 1：15, extraction time 2min.
Further, in step (6), the alcohol sedimentation is to use mass concentration to be settled for 95% ethanol, is carried
It is 1 to take the mass ratio of thing and ethanol：2.
Beneficial effect：
1st, the present invention passes through Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus gasseri, acidophilus breast bar
The mutual cooperation of bacterium, streptococcus thermophilus and Bifidobacterium, and special cultural method, sodium selenite can be converted into organic
Selenium, high conversion rate can guarantee that the addition of sodium selenite does not influence the growth of beneficial bacterium up to more than 95%, and each strain is by training
Cfu can keep higher level after supporting, and the cultural method of the selenium-enriched probioticses in the present invention is simpler, easy to operate.
Although the 2, Cranberry has good maintenance function to women's health, if directly edible Cranberry, then
The effect of it is played is not obvious enough.The present invention is first to be extracted to be answered with other raw materials again after obtaining extract by Cranberry
Match somebody with somebody, by the extraction to Cranberry, the materials such as phenolic compound more beneficial to product of the present invention, vitamin C can be obtained, made most
The effect of finally finished is more preferable.
3rd, the present invention either in storage or is eating obtained Cranberry extract and selenium-enriched probioticses microencapsulation
With when, all cause the reservation Du Genggao of contained active principle, effect is more notable.
4th, the present invention is first cultivated selenium-enriched probioticses compared with probiotic health food produces and is fabricated to microcapsules
Change selenium-enriched probioticses, obtain being rich in Organic Selenium probiotics, be a kind of safely and effectively selenium-supply food；Astragalus polyose, Chinese holly are added again
Qi polysaccharide, FOS, Cranberry extract are compounded so that this selenium-enriched probiotics drink not only sweet and sour taste, flavor color
Pool is all good, and good health care effect, and selenium-enriched probioticses can adjust gut flora, improve immune system, its Organic Selenium high conversion rate,
The existence of selenium is essentially Organic Selenium, can reach the effect of safe selenium-complement, is compounded with homemade astragalus polyose and LBP-X,
Can tonifying Qi and strengthen immunity；Organic Selenium also plays with the vitamin C being rich in Cranberry and cooperates with antioxidation, plays protection
Urogenital system, the specific function for safeguarding vaginal health and skin health.
Embodiment
With reference to specific embodiment, the present invention is described further.
Embodiment 1
A kind of selenium-enriched probioticses solid beverage, it is 3 in mass ratio by compound selenium-enriched probioticses, complex polysaccharide, FOS：
1：1 composition；The compound selenium-enriched probioticses are by quality by selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract
Than for 1：5 compositions；It is 1 in mass ratio by astragalus polyose and LBP-X that the complex polysaccharide, which is,：1 composition.
The preparation method of above-mentioned selenium-enriched probioticses solid beverage, comprises the following steps：
(1) Cranberry Feedstock treating：After the cleaning removal of impurities of Cranberry fresh fruit, the method crushed with liquid nitrogen cryogenics carries out powder
It is broken, comprise the following steps that：- 196 DEG C of liquid nitrogen are poured into Cranberry so that Cranberry quick freeze, be then placed in and crush at a high speed
Crushed in machine, the slurries and residue of Cranberry are collected after the completion of crushing, be well mixed, obtain Cranberry mixture；
(2) prepared by Cranberry extract：Using ultrasonic assistant ethanol extraction method, by Cranberry mixture and mass fraction
For 75% ethanol solution using weight ratio as 1：8 ratio is sufficiently mixed, and is placed into progress ultrasonic wave in ultrasonic extractor and is carried
Take, Extracting temperature is 48 DEG C, extraction time 4h, obtains Cranberry extract；
(3) Cranberry extract microencapsulation and dry powder processed：Cranberry extract obtained by step (2) is passed through into vacuum
Filtering, filtrate is taken to be positioned over sterile ventilation 24h, volatilize ethanol；Cranberry extract is prepared into using spray drying process again
Microencapsulation form, during preparation, wall material is maltodextrin, core wall ratio (v:w)5:3,170 DEG C of EAT, stream pressure
170kPa, feed rate 400mL/h, 50 DEG C of feeding temperature；The materials such as moisture are removed, obtain microcapsules Cranberry extract；
(4) selenium-enriched probioticses culture：In YEPD fluid nutrient mediums add Mixed Microbes cultivated, Mixed Microbes by etc. weight
Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus gasseri, lactobacillus acidophilus, streptococcus thermophilus composition,
Mixed Microbes addition weight accounts for the 5% of fluid nutrient medium weight；In logarithmic phase addition sodium selenite early stage (Na2SeO3, as inorganic
Selenium source), sodium selenite addition concentration is 12 μ g/ml, then carries out continuing to cultivate, and cultivation temperature is 36-37 DEG C, and incubation time is
30h, obtain selenium-enriched probioticses just nutrient solution；Bifidobacterium, cultivation temperature and time and selenium-rich benefit are cultivated in another incubator
Just nutrient solution is identical for raw bacterium；The Bifidobacterium that nutrient solution at the beginning of selenium-enriched probioticses is obtained with culture is using weight ratio as 2：1 ratio
Mixing, obtains selenium-enriched probioticses nutrient solution；
(5) selenium-enriched probioticses microencapsulation and dry powder processed：, will using ceramic membrane filter technology concentrated probiotics nutrient solution
Selenium-enriched probioticses nutrient solution carries out ceramic membrane filter, obtains concentrated probiotics nutrient solution, is 5 by mass ratio：3：2 concentration is prebiotic
Bacteria culture fluid, the sodium alginate that mass concentration is 2%, maltodextrin are sufficiently mixed, and are then freeze-dried, you can obtain selenium-rich
Probiotics viable bacteria freeze-dried powder；
(6) prepared by polysaccharide：
A, prepared by astragalus polyose：The Radix Astragali is ground into Ultramicro-powder with micronizer, addition cellulase is to Radix Astragali ultra micro
Powder is digested, enzymolysis time 2h, and the addition weight of cellulase is the 0.7% of Radix Astragali Ultramicro-powder weight；Again using flash
Extraction method extracts astragalus polyose, and twice, it be 80V to extract voltage every time for extraction, and the weight ratio of Radix Astragali Ultramicro-powder and water is 1：15, carry
It is 2min to take the time；It will filter, be concentrated in vacuo after extract solution mixes twice, and obtain astragalus polyose concentrate, will with alcohol sedimentation
Astragalus polyose concentrate is settled, separated, and the alcohol sedimentation is to use mass concentration to be settled for 95% ethanol, yellow
The mass ratio of astragalus polysaccharides concentrate and ethanol is 1：2,95% ethanol is poured into astragalus polyose concentrate, at the uniform velocity stirred clockwise
Mix, polysaccharide can be deposited, and be dried precipitation after sedimentation separation, as astragalus polyose；
B, prepared by LBP-X：After matrimony vine is ground into powder with high speed disintegrator, addition cellulase is to matrimony vine powder
Digested, enzymolysis time 2h, the addition weight of cellulase is the 0.7% of matrimony vine powder；Carried again using homogenate extraction method
Take LBP-X, twice, it be 80V to extract voltage every time for extraction, and the mass ratio of matrimony vine powder and water is 1：15, extraction time is
2min；Will filtering after extract solution mixes twice, be concentrated in vacuo, LBP-X concentrate is obtained, with alcohol sedimentation by LBP-X
Concentrate is settled, separated, and the alcohol sedimentation is to use mass concentration to be settled for 95% ethanol, and LBP-X is dense
The mass ratio of contracting liquid and ethanol is 1：2, precipitation is dried after sedimentation separation, you can obtain LBP-X；
(7) each component compounds：Selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract is fully mixed in proportion
Close, form compound selenium-enriched probioticses；Astragalus polyose and LBP-X are mixed in proportion, form complex polysaccharide；By compound selenium-rich
Probiotics, complex polysaccharide and FOS mix in proportion, you can obtain selenium-enriched probioticses solid beverage.
Embodiment 2
A kind of selenium-enriched probioticses solid beverage, it is 3 in mass ratio by compound selenium-enriched probioticses, complex polysaccharide, FOS：
1.2：1.2 composition；The compound selenium-enriched probioticses be by selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract by
Mass ratio is 1：4 compositions；It is 1 in mass ratio by astragalus polyose and LBP-X that the complex polysaccharide, which is,：1.5 composition.
The preparation method of above-mentioned selenium-enriched probioticses solid beverage, comprises the following steps：
(1) Cranberry Feedstock treating：After the cleaning removal of impurities of Cranberry fresh fruit, the method crushed with liquid nitrogen cryogenics carries out powder
It is broken, the slurries and residue of Cranberry are collected after the completion of crushing, is well mixed, obtains Cranberry mixture；
(2) prepared by Cranberry extract：Using ultrasonic assistant ethanol extraction method, by Cranberry mixture and mass fraction
For 75% ethanol solution using weight ratio as 1：7 ratio is sufficiently mixed, and is placed into progress ultrasonic wave in ultrasonic extractor and is carried
Take, Extracting temperature is 45 DEG C, extraction time 5h, obtains Cranberry extract；
(3) Cranberry extract microencapsulation and dry powder processed：Cranberry extract obtained by step (2) is passed through into vacuum
Filtering, takes filtrate to be positioned over sterile ventilation 20h, then Cranberry extract is prepared into microcapsules shape using spray drying process
Formula, wall material are maltodextrin, core wall ratio (v:w)5:3,170 DEG C of EAT, stream pressure 170kPa, feed rate
50 DEG C of 400mL/h, feeding temperature, the materials such as moisture are removed, obtain microcapsules Cranberry extract；
(4) selenium-enriched probioticses culture：In YEPD fluid nutrient mediums add Mixed Microbes cultivated, Mixed Microbes by etc. weight
Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus gasseri, lactobacillus acidophilus, streptococcus thermophilus composition,
Mixed Microbes addition accounts for the 4% of fluid nutrient medium weight；In logarithmic phase addition sodium selenite early stage (Na2SeO3, as inorganic selenium
Source), sodium selenite addition concentration is 10 μ g/ml, then carries out continuing to cultivate, and cultivation temperature is 36-37 DEG C, and incubation time is
28h, obtain selenium-enriched probioticses just nutrient solution；Bifidobacterium, cultivation temperature and time and selenium-rich benefit are cultivated in another incubator
Just nutrient solution is identical for raw bacterium；The Bifidobacterium that nutrient solution at the beginning of selenium-enriched probioticses is obtained with culture is using weight ratio as 3：1 is mixed
Close, obtain selenium-enriched probioticses nutrient solution；
(5) selenium-enriched probioticses microencapsulation and dry powder processed：, will using ceramic membrane filter technology concentrated probiotics nutrient solution
Selenium-enriched probioticses nutrient solution carries out ceramic membrane filter, obtains concentrated probiotics nutrient solution, is 5 by mass ratio：3：2 concentration is prebiotic
Bacteria culture fluid, the sodium alginate that mass concentration is 2%, maltodextrin are sufficiently mixed, and are then freeze-dried, you can obtain selenium-rich
Probiotics viable bacteria freeze-dried powder；
(6) prepared by polysaccharide：
A, prepared by astragalus polyose：The Radix Astragali is ground into Ultramicro-powder with micronizer, addition cellulase is to Radix Astragali ultra micro
Powder is digested, enzymolysis time 2h, and the addition weight of cellulase is the 0.6% of Radix Astragali Ultramicro-powder；Homogenate extraction is used again
Method extracts astragalus polyose, and twice, extracting parameter is the same as embodiment 1 for extraction；It will filter, be concentrated in vacuo after extract solution mixes twice, obtain
To astragalus polyose concentrate, astragalus polyose concentrate is settled with alcohol sedimentation, separated, the alcohol sedimentation is to use matter
The ethanol that amount concentration is 95% is settled, and the mass ratio of astragalus polyose concentrate and ethanol is 1：2, will precipitation after sedimentation separation
Drying, as astragalus polyose；
B, prepared by LBP-X：After matrimony vine is ground into powder with high speed disintegrator, addition cellulase is to matrimony vine powder
Digested, enzymolysis time 3h, the addition weight of cellulase is the 0.6% of matrimony vine powder；Carried again using homogenate extraction method
LBP-X is taken, twice, extracting parameter is the same as embodiment 1 for extraction；It will filter, be concentrated in vacuo after extract solution mixes twice, and obtain Chinese holly
Qi polysaccharide concentrate, LBP-X concentrate is settled with alcohol sedimentation, separated, the alcohol sedimentation is dense using quality
Spend and settled for 95% ethanol, the mass ratio of LBP-X concentrate and ethanol is 1：2, precipitation is dried after sedimentation separation
It is dry, you can to obtain LBP-X；
(7) each component compounds：Selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract is fully mixed in proportion
Close, form compound selenium-enriched probioticses；Astragalus polyose and LBP-X are mixed in proportion, form complex polysaccharide；By compound selenium-rich
Probiotics, complex polysaccharide and FOS mix in proportion, you can obtain selenium-enriched probioticses solid beverage.
Embodiment 3
A kind of selenium-enriched probioticses solid beverage, it is 3 in mass ratio by compound selenium-enriched probioticses, complex polysaccharide, FOS：
1.1：1.1 composition；The compound selenium-enriched probioticses be by selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract by
Mass ratio is 1：4.5 composition；It is 1 in mass ratio by astragalus polyose and LBP-X that the complex polysaccharide, which is,：1.2 composition.
The preparation method of above-mentioned selenium-enriched probioticses solid beverage, comprises the following steps：
(1) Cranberry Feedstock treating：After the cleaning removal of impurities of Cranberry fresh fruit, the method crushed with liquid nitrogen cryogenics carries out powder
It is broken, the slurries and residue of Cranberry are collected after the completion of crushing, is well mixed, obtains Cranberry mixture；
(2) prepared by Cranberry extract：Using ultrasonic assistant ethanol extraction method, by Cranberry mixture and mass fraction
For 75% ethanol solution using weight ratio as 1：8 ratio is sufficiently mixed, and is placed into progress ultrasonic wave in ultrasonic extractor and is carried
Take, Extracting temperature is 50 DEG C, extraction time 3h, obtains Cranberry extract；
(3) Cranberry extract microencapsulation and dry powder processed：Cranberry extract obtained by step (2) is passed through into vacuum
Filtering, filtrate is taken to be positioned over sterile ventilation 22h, volatilize ethanol；Cranberry extract is prepared into using spray drying process again
Microencapsulation form, wall material are maltodextrin, core wall ratio (v:w)5:3,170 DEG C of EAT, stream pressure 170kPa, charging
50 DEG C of flow 400mL/h, feeding temperature, the materials such as moisture are removed, obtain microcapsules Cranberry extract；
(4) selenium-enriched probioticses culture：In YEPD fluid nutrient mediums add Mixed Microbes cultivated, Mixed Microbes by etc. weight
Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus gasseri, lactobacillus acidophilus, streptococcus thermophilus composition,
Mixed Microbes addition accounts for the 4.5% of fluid nutrient medium weight；In logarithmic phase addition sodium selenite early stage (Na2SeO3, as inorganic
Selenium source), sodium selenite addition concentration is 15 μ g/ml, then carries out continuing to cultivate, and cultivation temperature is 36-37 DEG C, and incubation time is
29h, obtain selenium-enriched probioticses just nutrient solution；Bifidobacterium, cultivation temperature and time and selenium-rich benefit are cultivated in another incubator
Just nutrient solution is identical for raw bacterium；The Bifidobacterium that nutrient solution at the beginning of selenium-enriched probioticses is obtained with culture is using weight ratio as 2：1 ratio
Mixing, obtains selenium-enriched probioticses nutrient solution；
(5) selenium-enriched probioticses microencapsulation and dry powder processed：, will using ceramic membrane filter technology concentrated probiotics nutrient solution
Selenium-enriched probioticses nutrient solution carries out ceramic membrane filter, obtains concentrated probiotics nutrient solution, is 5 by mass ratio：3：2 concentration is prebiotic
Bacteria culture fluid, the sodium alginate that mass concentration is 2%, maltodextrin are sufficiently mixed, and are then freeze-dried, you can obtain selenium-rich
Probiotics viable bacteria freeze-dried powder；
(6) prepared by polysaccharide：
A, prepared by astragalus polyose：The Radix Astragali is ground into Ultramicro-powder with micronizer, addition cellulase is to Radix Astragali ultra micro
Powder is digested, enzymolysis time 2h, and the addition weight of cellulase is the 0.8% of Radix Astragali Ultramicro-powder；Homogenate extraction is used again
Method extracts astragalus polyose, and twice, extracting parameter is the same as embodiment 1 for extraction；It will filter, be concentrated in vacuo after extract solution mixes twice, obtain
To astragalus polyose concentrate, astragalus polyose concentrate is settled with alcohol sedimentation, separated, the alcohol sedimentation is to use matter
The ethanol that amount concentration is 95% is settled, and the mass ratio of astragalus polyose concentrate and ethanol is 1：2, will precipitation after sedimentation separation
Drying, as astragalus polyose；
B, prepared by LBP-X：After matrimony vine is ground into powder with high speed disintegrator, addition cellulase is to matrimony vine powder
Digested, enzymolysis time 2h, the addition weight of cellulase is the 0.8% of matrimony vine powder；Carried again using homogenate extraction method
LBP-X is taken, twice, extracting parameter is the same as embodiment 1 for extraction；It will filter, be concentrated in vacuo after extract solution mixes twice, and obtain Chinese holly
Qi polysaccharide concentrate, LBP-X concentrate is settled with alcohol sedimentation, separated, the alcohol sedimentation is dense using quality
Spend and settled for 95% ethanol, the mass ratio of LBP-X concentrate and ethanol is 1：2, precipitation is dried after sedimentation separation
It is dry, you can to obtain LBP-X；
(7) each component compounds：Selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract is fully mixed in proportion
Close, form compound selenium-enriched probioticses；Astragalus polyose and LBP-X are mixed in proportion, form complex polysaccharide；By compound selenium-rich
Probiotics, complex polysaccharide and FOS mix in proportion, you can obtain selenium-enriched probioticses solid beverage.
Embodiment 4
A kind of probiotics solid beverage, substantially the same manner as Example 1, difference is：Step in preparation process
(4) sodium selenite, is not added.
The finished product of solid beverage made from embodiment 1-4 is tested and analyzed, specific data are shown in Table 1.
Table 1
As it can be seen from table 1 the total Se content for the selenium-enriched probioticses solid beverage that embodiment 1-3 is prepared is very high, have
18-21mg/Kg, the conversion ratio that inorganic selenium changes into Organic Selenium is very high, up to more than 95%, and make the document of probiotic yogurt
The total Se content of product is 0.1-0.3mg/L, and conversion ratio only has 50%, it can be seen that, selenium-enriched probioticses are consolidated made of the present invention
Body drink is selenium-rich product, and inorganic selenium high conversion rate, and small-scale inorganic selenium can be neglected existing for final products, safely may be used
Lean on.
Each group of data is more or less the same it can be seen from cfu data comparisons of the embodiment 1-3 with each strain of embodiment 4, can
See, the present invention with the addition of sodium selenite and be cultivated, and not interfere with the growth of beneficial bacterium, each strain cfu after culture
Higher level can be kept.
Selenium-enriched probioticses solid beverage made from embodiment 1 is used for the compliance test result of women's health, it is specific as follows：
Trier is women, and suffers from disease of the genitourinary system, colpitis, the unsound one or more of skin,
Totally 50 people, age are 30-50 year to trier, average age 38 years old；Trial period is 3 months, and daily embodiment 1 prepares gained
Selenium-enriched probioticses solid beverage, daily 2 times, each 2g.After 3 months, the symptom of 48 people takes an evident turn for the better (wherein 15 people
Symptom disappear), 2 people are without significant change.As can be seen here, the selenium-enriched probioticses solid beverage pair of gained is prepared using embodiment 1
The treated effect of the problems such as female genitourinary system disease, colpitis, unhealthy skin is up to 96%.It is in addition, all
Trier represents that this product is in good taste, and convenient to take, and the effect of this product is satisfied with.The product of the present invention is tried
User's likes and approves.
It is below several typical cases example on probation in 50 triers：
Example 1 on probation：Mrs Wang, 35 years old, urinary system infection contamination, there are the symptoms such as urgent urination, frequent micturition, odynuria, mood anxiety, sleep
It is bad；After on probation 1 month, above symptom is relieved, and is fully recovered after 3 months, and all symptoms disappear, and sleep quality improves, spirit
Condition improvement is a lot.
Example 2 on probation：Ms Zhang, 32 years old, with vaginitis, leukorrhea had a peculiar smell, and complexion is obscure；After two weeks on probation
Above symptom is reduced, and is fully recovered after 3 months, and complexion recovers gloss.
Example 3 on probation：Miss Wu, 50 years old, there is a leukorrhea, the scorching hot itch of private parts, the symptom such as vagina pain during urine；It is on probation
After 20 days, above symptom is clearly better, and after 3 months, above symptom is wholly absent, and is got well, gather colour.
Although the present invention is disclosed as above with preferred embodiment, so it is not intended to limiting the invention, any this area skill
Art personnel, without departing from the spirit and scope of the present invention, when a little modification and perfect, therefore the protection model of the present invention can be made
Enclose to work as and be defined by what claims were defined.
Claims (10)
- A kind of 1. selenium-enriched probioticses solid beverage, it is characterised in that：Pressed by compound selenium-enriched probioticses, complex polysaccharide, FOS Mass ratio is 3：1-1.2：1-1.2 composition；The compound selenium-enriched probioticses are to be in mass ratio by selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract 1：4-5 is formed；It is 1 in mass ratio by astragalus polyose and LBP-X that the complex polysaccharide, which is,：1-1.5 composition.
- 2. selenium-enriched probioticses solid beverage as claimed in claim 1, it is characterised in that：By compound selenium-enriched probioticses, compound more Sugar, FOS are 3 in mass ratio：1：1 composition；The compound selenium-enriched probioticses are to be in mass ratio by selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract 1：5 compositions；It is 1 in mass ratio by astragalus polyose and LBP-X that the complex polysaccharide, which is,：1 composition.
- 3. the preparation method of selenium-enriched probioticses solid beverage as claimed in claim 1 or 2, it is characterised in that comprise the following steps：(1) Cranberry Feedstock treating：After the cleaning removal of impurities of Cranberry fresh fruit, crushed, collect the slurries and residue of Cranberry, It is well mixed, obtain Cranberry mixture；(2) prepared by Cranberry extract：Cranberry mixture is sufficiently mixed with mass fraction for 75% ethanol solution, then entered Row ultrasonic wave extraction, Extracting temperature are 45-50 DEG C, extraction time 3-5h, obtain Cranberry extract；(3) Cranberry extract microencapsulation and dry powder processed：Cranberry extract obtained by step (2) is subjected to vacuum mistake Filter, takes filtrate to be positioned over sterile ventilation 20-24h, then filtrate is prepared into microencapsulation form, obtains the extraction of microcapsules Cranberry Thing；(4) selenium-enriched probioticses culture：Add Mixed Microbes in YEPD fluid nutrient mediums to be cultivated, Mixed Microbes are by plant breast bar Bacterium, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus gasseri, lactobacillus acidophilus, streptococcus thermophilus composition, Mixed Microbes addition Weight accounts for the 4-5% of YEPD fluid nutrient medium weight；In logarithmic phase addition early stage sodium selenite, sodium selenite addition concentration is 10-15 μ g/ml, then carry out continuing to cultivate, cultivation temperature is 36-37 DEG C, incubation time 28-30h, obtains selenium-enriched probioticses First nutrient solution；By nutrient solution at the beginning of selenium-enriched probioticses and Bifidobacterium using weight ratio as 2-3：1 ratio mixing, it is prebiotic to obtain selenium-rich Bacteria culture fluid；(5) selenium-enriched probioticses microencapsulation and dry powder processed：Selenium-enriched probioticses nutrient solution is subjected to ceramic membrane filter, concentrated Probiotic's culture liquid, concentrated probiotics nutrient solution, the sodium alginate that mass concentration is 2%, maltodextrin are sufficiently mixed, so After be freeze-dried, you can obtain selenium-enriched probioticses viable bacteria freeze-dried powder；(6) prepared by polysaccharide：A, prepared by astragalus polyose：The Radix Astragali is ground into Ultramicro-powder, addition cellulase digests to Radix Astragali Ultramicro-powder, during enzymolysis Between be 2-3h, the addition weight of cellulase is the 0.6-0.8% of Radix Astragali Ultramicro-powder；It is more using the homogenate extraction method extraction Radix Astragali again Sugar, extraction twice, will filter, concentration after extract solution mixes twice, and obtain astragalus polyose concentrate, with alcohol sedimentation that the Radix Astragali is more Sugared concentrate is settled, separated, and precipitation is dried, you can obtain astragalus polyose；B, prepared by LBP-X：After matrimony vine is ground into powder, the same astragalus polyose of other steps, you can obtain LBP-X；(7) each component compounds：Selenium-enriched probioticses viable bacteria freeze-dried powder and microcapsules Cranberry extract are sufficiently mixed in proportion, shape Into compound selenium-enriched probioticses；Astragalus polyose and LBP-X are mixed in proportion, form complex polysaccharide；Compound selenium-rich is prebiotic Bacterium, complex polysaccharide and FOS mix in proportion, you can obtain selenium-enriched probioticses solid beverage.
- 4. the preparation method of selenium-enriched probioticses solid beverage as claimed in claim 3, it is characterised in that：It is described in step (1) Cranberry is that the method crushed with liquid nitrogen cryogenics is crushed, and is specially：- 196 DEG C of liquid nitrogen are poured into Cranberry so that climing to get over Certain kind of berries quick freeze, is then placed in high speed disintegrator and is crushed.
- 5. the preparation method of selenium-enriched probioticses solid beverage as claimed in claim 3, it is characterised in that：It is climing to get in step (2) The weight ratio for the ethanol solution that certain kind of berries mixture is 75% with mass fraction is 1：7-8, Extracting temperature are 48 DEG C, and extraction time is 4h。
- 6. the preparation method of selenium-enriched probioticses solid beverage as claimed in claim 3, it is characterised in that：It is described in step (3) Microcapsules Cranberry extract is that Cranberry extract filtrate is prepared into microencapsulation form, concrete operations ginseng using spray drying Number is as follows：Wall material is maltodextrin, and core wall ratio is v：W=5：3,170 DEG C of EAT, stream pressure 170KPa, enter stream Measure 400mL/h, 50 DEG C of feeding temperature.
- 7. the preparation method of selenium-enriched probioticses solid beverage as claimed in claim 3, it is characterised in that：It is described in step (4) Mixed Microbes by etc. the Lactobacillus plantarum of weight, Lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus gasseri, lactobacillus acidophilus, thermophilic Hot streptococcus composition.
- 8. the preparation method of selenium-enriched probioticses solid beverage as claimed in claim 3, it is characterised in that：It is described in step (5) Sodium alginate that concentrated probiotics nutrient solution, mass concentration are 2%, the mass ratio of maltodextrin are 5：3：2.
- 9. the preparation method of selenium-enriched probioticses solid beverage as claimed in claim 3, it is characterised in that：It is described in step (6) The extraction voltage of homogenate extraction is that the mass ratio of 80V, the Radix Astragali or matrimony vine and water is 1：15, extraction time 2min.
- 10. the preparation method of selenium-enriched probioticses solid beverage as claimed in claim 3, it is characterised in that：In step (6), institute It is to use mass concentration to be settled for 95% ethanol to state alcohol sedimentation, and the mass ratio of extract and ethanol is 1：2.
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Cited By (1)
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| CN109123295A (en) * | 2018-07-24 | 2019-01-04 | 北京奥维森基因健康科技有限公司 | A kind of probiotics solid beverage and preparation method thereof |
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